Rmu_sc0000071.1_g000003

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000071.1
OG0003710
Physical Location & Seq
Forward (+)
10351 .. 14777
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ctgtggtccttgccggatcgggtcctcgggtccagcggcgagttgaggctgctgaacttgcaatggtttgccgcatttgagaaccagatgaagaagaagctagagaggtctagggtgagcttctccgacgagttggatccgacggagtcggaggggaagaggaagaacacagcgatggagagggggaggaaagcgacggtggtggatatacaaagggaatttctctccaaaacgcgcgaaatacaaacccaccgcctctctctctacacagggattgttctgataatggcggaggcaccgacgagtccggccggcgggagccacgagagcggcggcgagcagagcccgcagaacgggagcagcagcaacgtgagggagcaggacaggtacctcccaatcgcgaacatcagtcggattatgaagaaggcgctgccgcaaaacggcaagatcgccaaggacgctaaggacactgttcaggaatgcgtctccgaattcatcagcttcgtcaccagcgaggccagcgataagtgccagaaggagaagaggaagacgattaacggtgacgatttgctgtgggcgatggctacgttagggtttgaggactatattgagccgctcaggatttacttggctaggtacagggagttggagggtgatgcaaagggttctgcaaggggtggagaagggtctgctaaagggaatcctgttggagctatgactgggcaaaattcacagtttgttcatcagccaccactgaactacgtcaataaccaagaggattattatgtgcctttgaacaatatggggaatggaaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
ko04612 ko05152 ko05166 map04612 map05152 map05166
Pfam Domains
Protein Families
CBFD_NFYB_HMF

Protein Analysis

272

Amino Acids

30.29

Weight (kDa)

7.04

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 130 - 194 7e-28 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 387
AccB1I GGYRCC 2 cut(s) 295, 387
AccBSI CCGCTC 2 cut(s) 330, 616
AccII CGCG 3 cut(s) 235, 237, 401
AclWI GGATC 3 cut(s) 24, 131, 144
AcoI YGGCCR 1 cut(s) 309
AcsI RAATTY 3 cut(s) 218, 491, 727
AfaI GTAC 2 cut(s) 389, 638
AfiI CCNNNNNNNGG 3 cut(s) 314, 353, 440
AgsI TTSAA 1 cut(s) 796
AluBI AGCT 4 cut(s) 100, 120, 501, 713
AluI AGCT 4 cut(s) 100, 120, 501, 713
Alw26I GTCTC 1 cut(s) 490
AlwI GGATC 3 cut(s) 24, 131, 144
Ama87I CYCGRG 1 cut(s) 26
AoxI GGCC 2 cut(s) 309, 516
ApeKI GCWGC 4 cut(s) 49, 360, 363, 430
ApoI RAATTY 3 cut(s) 218, 491, 727
Asp718I GGTACC 1 cut(s) 387
AspLEI GCGC 2 cut(s) 237, 430
AspS9I GGNCC 3 cut(s) 6, 22, 30
AsuHPI GGTGA 4 cut(s) 127, 499, 572, 665
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 3 cut(s) 6, 22, 30
BamHI GGATCC 1 cut(s) 136
BanI GGYRCC 2 cut(s) 295, 387
BanII GRGCYC 1 cut(s) 347
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 554
BbvI GCAGC 4 cut(s) 36, 372, 375, 417
BccI CCATC 2 cut(s) 169, 574
BceAI ACGGC 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 490
BfaI CTAG 3 cut(s) 101, 111, 633
BfoI RGCGCY 1 cut(s) 431
Bme18I GGWCC 3 cut(s) 6, 22, 30
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 3 cut(s) 6, 22, 30
BmiI GGNNCC 6 cut(s) 23, 31, 138, 297, 320, 389
BmrI ACTGGG 1 cut(s) 729
BmsI GCATC 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 554
Bpu10I CCTNAGC 2 cut(s) 462, 617
BsaJI CCNNGG 2 cut(s) 25, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 353, 440
Bse118I RCCGGY 1 cut(s) 311
Bse1I ACTGG 1 cut(s) 724
Bse3DI GCAATG 1 cut(s) 68
BseDI CCNNGG 2 cut(s) 25, 453
BseLI CCNNNNNNNGG 3 cut(s) 314, 353, 440
BseMI GCAATG 1 cut(s) 68
BseMII CTCAG 1 cut(s) 631
BseNI ACTGG 1 cut(s) 724
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 4 cut(s) 36, 372, 375, 417
Bsh1236I CGCG 3 cut(s) 235, 237, 401
Bsh1285I CGRYCG 1 cut(s) 312
BshFI GGCC 2 cut(s) 311, 518
BshNI GGYRCC 2 cut(s) 295, 387
BsiEI CGRYCG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 3 cut(s) 14, 308, 312
BslI CCNNNNNNNGG 3 cut(s) 314, 353, 440
BsmAI GTCTC 1 cut(s) 490
BsmBI CGTCTC 1 cut(s) 490
BsmI GAATGC 1 cut(s) 485
BsnI GGCC 2 cut(s) 311, 518
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 347
Bsp143I GATC 3 cut(s) 16, 136, 447
Bsp68I TCGCGA 1 cut(s) 401
BspANI GGCC 2 cut(s) 311, 518
BspCNI CTCAG 1 cut(s) 630
BspFNI CGCG 3 cut(s) 235, 237, 401
BspLI GGNNCC 6 cut(s) 23, 31, 138, 297, 320, 389
BspPI GGATC 3 cut(s) 24, 131, 144
BspT107I GGYRCC 2 cut(s) 295, 387
BsrBI CCGCTC 2 cut(s) 330, 616
BsrDI GCAATG 1 cut(s) 68
BsrFI RCCGGY 1 cut(s) 311
BsrI ACTGG 1 cut(s) 724
BssAI RCCGGY 1 cut(s) 311
BssECI CCNNGG 2 cut(s) 25, 453
BssMI GATC 3 cut(s) 16, 136, 447
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 323
Bst4CI ACNGT 4 cut(s) 199, 472, 560, 735
Bst6I CTCTTC 2 cut(s) 152, 536
BstC8I GCNNGC 4 cut(s) 313, 338, 347, 520
BstDEI CTNAG 2 cut(s) 462, 617
BstFNI CGCG 3 cut(s) 235, 237, 401
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 237, 430
BstKTI GATC 3 cut(s) 19, 139, 450
BstMAI GTCTC 1 cut(s) 490
BstMBI GATC 3 cut(s) 16, 136, 447
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 7 cut(s) 58, 327, 342, 346, 458, 519, 528
BstUI CGCG 3 cut(s) 235, 237, 401
BstV1I GCAGC 4 cut(s) 36, 372, 375, 417
BstV2I GAAGAC 1 cut(s) 554
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 2 cut(s) 311, 518
BtgZI GCGATG 2 cut(s) 188, 593
BtsIMutI CAGTG 2 cut(s) 468, 752
BtuMI TCGCGA 1 cut(s) 401
Cac8I GCNNGC 4 cut(s) 313, 338, 347, 520
CfoI GCGC 2 cut(s) 237, 430
Cfr10I RCCGGY 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 6, 22, 30
CseI GACGC 2 cut(s) 467, 472
Csp6I GTAC 2 cut(s) 388, 637
CviQI GTAC 2 cut(s) 388, 637
DdeI CTNAG 2 cut(s) 462, 617
DpnI GATC 3 cut(s) 18, 138, 449
DpnII GATC 3 cut(s) 16, 136, 447
EaeI YGGCCR 1 cut(s) 309
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 2 cut(s) 152, 536
EarI CTCTTC 2 cut(s) 152, 536
EciI GGCGGA 1 cut(s) 305
EclXI CGGCCG 1 cut(s) 309
Eco130I CCWWGG 1 cut(s) 453
Eco24I GRGCYC 1 cut(s) 347
Eco47I GGWCC 3 cut(s) 6, 22, 30
Eco52I CGGCCG 1 cut(s) 309
Eco88I CYCGRG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 22
EcoRI GAATTC 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 453
EcoT38I GRGCYC 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 490
FaiI YATR 6 cut(s) 209, 419, 606, 716, 786, 803
FauI CCCGC 2 cut(s) 308, 354
FriOI GRGCYC 1 cut(s) 347
FspBI CTAG 3 cut(s) 101, 111, 633
GlaI GCGC 2 cut(s) 236, 429
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 2 cut(s) 311, 518
HapII CCGG 3 cut(s) 14, 308, 312
HgaI GACGC 2 cut(s) 467, 472
HhaI GCGC 2 cut(s) 237, 430
Hin6I GCGC 2 cut(s) 235, 428
HinP1I GCGC 2 cut(s) 235, 428
HinfI GANTC 3 cut(s) 146, 304, 700
HpaII CCGG 3 cut(s) 14, 308, 312
HphI GGTGA 4 cut(s) 127, 499, 572, 665
Hpy188I TCNGA 6 cut(s) 127, 141, 151, 282, 414, 490
Hpy188III TCNNGA 3 cut(s) 400, 476, 619
Hpy99I CGWCG 4 cut(s) 131, 145, 199, 304
HpyAV CCTTC 3 cut(s) 418, 529, 677
HpyCH4III ACNGT 4 cut(s) 199, 472, 560, 735
HpyCH4IV ACGT 3 cut(s) 369, 587, 762
HpyCH4V TGCA 3 cut(s) 61, 659, 671
HpyF10VI GCNNNNNNNGC 7 cut(s) 58, 327, 342, 346, 458, 519, 528
HpyF3I CTNAG 2 cut(s) 462, 617
HpySE526I ACGT 3 cut(s) 369, 587, 762
HspAI GCGC 2 cut(s) 235, 428
KpnI GGTACC 1 cut(s) 391
KroI GCCGGC 1 cut(s) 311
KroNI GCCGGC 1 cut(s) 313
Kzo9I GATC 3 cut(s) 16, 136, 447
LmnI GCTCC 4 cut(s) 318, 357, 376, 710
Lsp1109I GCAGC 4 cut(s) 36, 372, 375, 417
LweI GCATC 1 cut(s) 646
MaeI CTAG 3 cut(s) 101, 111, 633
MaeII ACGT 3 cut(s) 369, 587, 762
MaeIII GTNAC 2 cut(s) 505, 560
MalI GATC 3 cut(s) 18, 138, 449
MbiI CCGCTC 2 cut(s) 330, 616
MboI GATC 3 cut(s) 16, 136, 447
MboII GAAGA 7 cut(s) 103, 106, 169, 175, 433, 553, 559
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 4 cut(s) 218, 491, 727, 814
MlyI GAGTC 2 cut(s) 155, 313
MmeI TCCRAC 7 cut(s) 114, 129, 150, 164, 392, 627, 688
MroNI GCCGGC 1 cut(s) 311
MseI TTAA 1 cut(s) 555
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 36
MspI CCGG 3 cut(s) 14, 308, 312
Mva1269I GAATGC 1 cut(s) 485
MvnI CGCG 3 cut(s) 235, 237, 401
MwoI GCNNNNNNNGC 7 cut(s) 58, 327, 342, 346, 458, 519, 528
NaeI GCCGGC 1 cut(s) 313
NdeII GATC 3 cut(s) 16, 136, 447
NgoMIV GCCGGC 1 cut(s) 311
NlaIV GGNNCC 6 cut(s) 23, 31, 138, 297, 320, 389
NmuCI GTSAC 2 cut(s) 505, 560
NruI TCGCGA 1 cut(s) 401
PcsI WCGNNNNNNNCGW 1 cut(s) 510
PctI GAATGC 1 cut(s) 485
PdiI GCCGGC 1 cut(s) 313
PfeI GAWTC 1 cut(s) 700
PflFI GACNNNGTC 1 cut(s) 145
PleI GAGTC 2 cut(s) 154, 312
PpsI GAGTC 2 cut(s) 154, 312
PpuMI RGGWCCY 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 22
PspN4I GGNNCC 6 cut(s) 23, 31, 138, 297, 320, 389
PspPI GGNCC 3 cut(s) 6, 22, 30
PspPPI RGGWCCY 1 cut(s) 22
PsuI RGATCY 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 145
RruI TCGCGA 1 cut(s) 401
RsaI GTAC 2 cut(s) 389, 638
RsaNI GTAC 2 cut(s) 388, 637
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 1 cut(s) 555
Sau3AI GATC 3 cut(s) 16, 136, 447
Sau96I GGNCC 3 cut(s) 6, 22, 30
SchI GAGTC 2 cut(s) 155, 313
SduI GDGCHC 1 cut(s) 347
SfaNI GCATC 1 cut(s) 646
SinI GGWCC 3 cut(s) 6, 22, 30
SmiMI CAYNNNNRTG 1 cut(s) 173
Sse9I AATT 4 cut(s) 218, 491, 727, 814
SspMI CTAG 3 cut(s) 101, 111, 633
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 4 cut(s) 199, 472, 560, 735
TaiI ACGT 3 cut(s) 372, 590, 765
TasI AATT 4 cut(s) 218, 491, 727, 814
TauI GCSGC 6 cut(s) 39, 74, 333, 336, 436, 616
TfiI GAWTC 1 cut(s) 700
Tru1I TTAA 1 cut(s) 555
Tru9I TTAA 1 cut(s) 555
TscAI CASTG 2 cut(s) 475, 759
TseFI GTSAC 2 cut(s) 505, 560
TseI GCWGC 4 cut(s) 49, 360, 363, 430
Tsp45I GTSAC 2 cut(s) 505, 560
TspDTI ATGAA 4 cut(s) 104, 434, 484, 731
TspGWI ACGGA 1 cut(s) 158
TspRI CASTG 2 cut(s) 475, 759
Tth111I GACNNNGTC 1 cut(s) 145
VpaK11BI GGWCC 3 cut(s) 6, 22, 30
XapI RAATTY 3 cut(s) 218, 491, 727
XspI CTAG 3 cut(s) 101, 111, 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.