Rmu_sc0000322.1_g000032

Diphthamide biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000322.1
OG0010480
Physical Location & Seq
Forward (+)
127613 .. 129819
2207 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 888 bp
atggtggataccacgaagaaaatctatgctggtcccactgggagtgccaaaatgtttggctccaaaactggttcagctagcaaacagcctcttttgagtgcgcaggcgtgccagcaccgtgggattgagcagagcaatcaccgggaagtaggagaaagtagaatgagcatagcaatcaccgggaagtacgagaaagcacggggtttgctaaagcgtgactttagcttcactgggttgcaagggccttcaaataacagaggaaatacgcttggaagtttggaccccgccgctgctctgaagtttaaacaagaaacccagttttccgacgaccagctcaccgccgatggaggaggcgcaaaagccaagtctcgctcttctcggcgactcagaacagcgaaagccacaagcgccgaagcggttcgtccggagccaaatcccggactcaatactccacgacccggctctcaacgccgccataagcctcctcccctccaactaccacttcgaagtccacaaatgcgtctggcgcgtccgctccacgaacgcctcgcgcgtcgctctccagctgccggagggcctcctgatgtactccctcgtcctctccgatattctctccaccttcggaggcgcctcccagtgcttcgttctcggcgacgccacttacggcgcaccgtccatctcaacctccaaaaccctctccctaaaaccctagtcctcgctggaacaatccagttcgcttcggcgattcgagctgccaagcccgagcttgaagcccggggtttcaaggtcttggtcccacagtctaagcccttgtccgccagcgaggttttgggctgtacagccccgaaaatctcacgaaaccacggtgaaagtactagaatggcctag

Protein Analysis

295

Amino Acids

32.23

Weight (kDa)

11.63

Isoelectric Point (pI)

68.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Diphthamide_syn PF01866 223 - 287 2.8e-09 Putative diphthamide synthesis protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 102
AccB1I GGYRCC 1 cut(s) 627
AccBSI CCGCTC 1 cut(s) 535
AccII CGCG 3 cut(s) 529, 551, 553
AccIII TCCGGA 1 cut(s) 424
AciI CCGC 7 cut(s) 285, 288, 339, 416, 472, 533, 816
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 2 cut(s) 628, 655
AfaI GTAC 4 cut(s) 188, 588, 838, 874
AfiI CCNNNNNNNGG 3 cut(s) 437, 458, 569
AgsI TTSAA 3 cut(s) 249, 770, 784
AjuI GAANNNNNNNTTGG 2 cut(s) 486, 518
AluBI AGCT 6 cut(s) 77, 225, 334, 566, 752, 766
AluI AGCT 6 cut(s) 77, 225, 334, 566, 752, 766
Alw26I GTCTC 1 cut(s) 372
Ama87I CYCGRG 2 cut(s) 761, 774
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 3 cut(s) 242, 575, 882
ApeKI GCWGC 3 cut(s) 290, 566, 752
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
Asp700I GAANNNNTTC 1 cut(s) 417
AspLEI GCGC 7 cut(s) 103, 356, 410, 529, 553, 630, 669
AspS9I GGNCC 5 cut(s) 32, 242, 280, 575, 793
AsuC2I CCSGG 6 cut(s) 143, 181, 438, 459, 775, 776
AsuHPI GGTGA 4 cut(s) 131, 169, 328, 878
AsuII TTCGAA 1 cut(s) 505
AsuNHI GCTAGC 1 cut(s) 77
AvaI CYCGRG 2 cut(s) 761, 774
AvaII GGWCC 3 cut(s) 32, 280, 793
BanI GGYRCC 1 cut(s) 627
BbvI GCAGC 3 cut(s) 277, 553, 739
BccI CCATC 2 cut(s) 338, 684
BceAI ACGGC 1 cut(s) 680
BcnI CCSGG 6 cut(s) 143, 181, 438, 459, 775, 776
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 4 cut(s) 78, 710, 876, 886
BfoI RGCGCY 2 cut(s) 411, 631
BisI GCNGC 5 cut(s) 288, 291, 472, 567, 753
BlsI GCNGC 5 cut(s) 289, 292, 473, 568, 754
BmcAI AGTACT 1 cut(s) 874
Bme1390I CCNGG 6 cut(s) 143, 181, 438, 459, 775, 776
Bme18I GGWCC 3 cut(s) 32, 280, 793
BmeT110I CYCGRG 2 cut(s) 761, 774
BmgT120I GGNCC 5 cut(s) 32, 242, 280, 575, 793
BmiI GGNNCC 6 cut(s) 34, 61, 282, 429, 629, 795
BmrFI CCNGG 6 cut(s) 143, 181, 438, 459, 775, 776
BmrI ACTGGG 4 cut(s) 48, 240, 310, 629
BmtI GCTAGC 1 cut(s) 81
BmuI ACTGGG 4 cut(s) 48, 240, 310, 629
BpmI CTGGAG 1 cut(s) 546
Bpu14I TTCGAA 1 cut(s) 505
BpuMI CCSGG 6 cut(s) 143, 181, 438, 459, 775, 776
BsaHI GRCGYC 2 cut(s) 628, 655
BsaJI CCNNGG 4 cut(s) 118, 774, 775, 862
BsaWI WCCGGW 1 cut(s) 424
Bsc4I CCNNNNNNNGG 3 cut(s) 437, 458, 569
Bse1I ACTGG 6 cut(s) 43, 73, 235, 316, 635, 730
BseAI TCCGGA 1 cut(s) 424
BseDI CCNNGG 4 cut(s) 118, 774, 775, 862
BseLI CCNNNNNNNGG 3 cut(s) 437, 458, 569
BseMII CTCAG 1 cut(s) 400
BseNI ACTGG 6 cut(s) 43, 73, 235, 316, 635, 730
BseRI GAGGAG 2 cut(s) 363, 474
BseXI GCAGC 3 cut(s) 277, 553, 739
Bsh1236I CGCG 3 cut(s) 529, 551, 553
BshFI GGCC 3 cut(s) 244, 577, 884
BshNI GGYRCC 1 cut(s) 627
BsiHKCI CYCGRG 2 cut(s) 761, 774
BsiSI CCGG 7 cut(s) 142, 180, 425, 438, 459, 570, 775
BslFI GGGAC 2 cut(s) 18, 779
BslI CCNNNNNNNGG 3 cut(s) 437, 458, 569
BsmAI GTCTC 1 cut(s) 372
BsmFI GGGAC 2 cut(s) 18, 779
BsnI GGCC 3 cut(s) 244, 577, 884
BsoBI CYCGRG 2 cut(s) 761, 774
Bsp119I TTCGAA 1 cut(s) 505
Bsp13I TCCGGA 1 cut(s) 424
Bsp1407I TGTACA 1 cut(s) 836
BspACI CCGC 7 cut(s) 285, 288, 339, 416, 472, 533, 816
BspANI GGCC 3 cut(s) 244, 577, 884
BspCNI CTCAG 1 cut(s) 399
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 3 cut(s) 529, 551, 553
BspLI GGNNCC 6 cut(s) 34, 61, 282, 429, 629, 795
BspOI GCTAGC 1 cut(s) 81
BspQI GCTCTTC 1 cut(s) 379
BspT104I TTCGAA 1 cut(s) 505
BspT107I GGYRCC 1 cut(s) 627
BsrBI CCGCTC 1 cut(s) 535
BsrGI TGTACA 1 cut(s) 836
BsrI ACTGG 6 cut(s) 43, 73, 235, 316, 635, 730
BssECI CCNNGG 4 cut(s) 118, 774, 775, 862
BssNI GRCGYC 2 cut(s) 628, 655
Bst4CI ACNGT 4 cut(s) 119, 673, 801, 866
Bst6I CTCTTC 1 cut(s) 379
BstACI GRCGYC 2 cut(s) 628, 655
BstAUI TGTACA 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 505
BstC8I GCNNGC 5 cut(s) 79, 105, 109, 113, 820
BstDEI CTNAG 2 cut(s) 386, 804
BstDSI CCRYGG 2 cut(s) 118, 862
BstFNI CGCG 3 cut(s) 529, 551, 553
BstH2I RGCGCY 2 cut(s) 411, 631
BstHHI GCGC 7 cut(s) 103, 356, 410, 529, 553, 630, 669
BstMAI GTCTC 1 cut(s) 372
BstMWI GCNNNNNNNGC 4 cut(s) 407, 468, 526, 749
BstSCI CCNGG 6 cut(s) 141, 179, 436, 457, 773, 774
BstUI CGCG 3 cut(s) 529, 551, 553
BstV1I GCAGC 3 cut(s) 277, 553, 739
BstXI CCANNNNNNTGG 1 cut(s) 119
BsuRI GGCC 3 cut(s) 244, 577, 884
BtgI CCRYGG 2 cut(s) 118, 862
BtsIMutI CAGTG 3 cut(s) 36, 228, 642
Cac8I GCNNGC 5 cut(s) 79, 105, 109, 113, 820
CfoI GCGC 7 cut(s) 103, 356, 410, 529, 553, 630, 669
Cfr13I GGNCC 5 cut(s) 32, 242, 280, 575, 793
Cfr9I CCCGGG 1 cut(s) 774
CseI GACGC 4 cut(s) 509, 518, 542, 663
Csp6I GTAC 4 cut(s) 187, 587, 837, 873
CviQI GTAC 4 cut(s) 187, 587, 837, 873
DdeI CTNAG 2 cut(s) 386, 804
DinI GGCGCC 1 cut(s) 629
DraI TTTAAA 1 cut(s) 304
Eam1104I CTCTTC 1 cut(s) 379
EarI CTCTTC 1 cut(s) 379
EciI GGCGGA 1 cut(s) 805
Eco47I GGWCC 3 cut(s) 32, 280, 793
Eco57I CTGAAG 1 cut(s) 317
Eco88I CYCGRG 2 cut(s) 761, 774
EcoO109I RGGNCCY 2 cut(s) 242, 575
EgeI GGCGCC 1 cut(s) 629
EheI GGCGCC 1 cut(s) 629
FaiI YATR 3 cut(s) 27, 170, 477
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FaqI GGGAC 2 cut(s) 18, 779
FauI CCCGC 1 cut(s) 292
Fnu4HI GCNGC 5 cut(s) 288, 291, 472, 567, 753
Fsp4HI GCNGC 5 cut(s) 288, 291, 472, 567, 753
FspBI CTAG 4 cut(s) 78, 710, 876, 886
FspI TGCGCA 1 cut(s) 102
GlaI GCGC 7 cut(s) 102, 355, 409, 528, 552, 629, 668
GluI GCNGC 5 cut(s) 288, 291, 472, 567, 753
GsuI CTGGAG 1 cut(s) 546
HaeII RGCGCY 2 cut(s) 411, 631
HaeIII GGCC 3 cut(s) 244, 577, 884
HapII CCGG 7 cut(s) 142, 180, 425, 438, 459, 570, 775
HgaI GACGC 4 cut(s) 509, 518, 542, 663
HhaI GCGC 7 cut(s) 103, 356, 410, 529, 553, 630, 669
Hin1I GRCGYC 2 cut(s) 628, 655
Hin6I GCGC 7 cut(s) 101, 354, 408, 527, 551, 628, 667
HinP1I GCGC 7 cut(s) 101, 354, 408, 527, 551, 628, 667
HinfI GANTC 3 cut(s) 384, 441, 745
HpaII CCGG 7 cut(s) 142, 180, 425, 438, 459, 570, 775
HphI GGTGA 4 cut(s) 131, 169, 328, 878
Hpy166II GTNNAC 1 cut(s) 512
Hpy188I TCNGA 5 cut(s) 297, 325, 389, 605, 624
Hpy188III TCNNGA 3 cut(s) 425, 581, 855
Hpy8I GTNNAC 1 cut(s) 512
Hpy99I CGWCG 3 cut(s) 329, 558, 657
HpyAV CCTTC 2 cut(s) 255, 629
HpyCH4III ACNGT 4 cut(s) 119, 673, 801, 866
HpyCH4V TGCA 1 cut(s) 238
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 468, 526, 749
HpyF3I CTNAG 2 cut(s) 386, 804
Hsp92I GRCGYC 2 cut(s) 628, 655
HspAI GCGC 7 cut(s) 101, 354, 408, 527, 551, 628, 667
KasI GGCGCC 1 cut(s) 627
Kpn2I TCCGGA 1 cut(s) 424
LguI GCTCTTC 1 cut(s) 379
LmnI GCTCC 3 cut(s) 65, 427, 540
Lsp1109I GCAGC 3 cut(s) 277, 553, 739
MaeI CTAG 4 cut(s) 78, 710, 876, 886
MaeIII GTNAC 1 cut(s) 215
MbiI CCGCTC 1 cut(s) 535
MboII GAAGA 2 cut(s) 28, 366
Mly113I GGCGCC 1 cut(s) 628
MlyI GAGTC 2 cut(s) 378, 435
MmeI TCCRAC 2 cut(s) 348, 517
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 417
MseI TTAA 1 cut(s) 303
MspA1I CMGCKG 2 cut(s) 290, 566
MspI CCGG 7 cut(s) 142, 180, 425, 438, 459, 570, 775
MspR9I CCNGG 6 cut(s) 143, 181, 438, 459, 775, 776
MssI GTTTAAAC 1 cut(s) 304
MvnI CGCG 3 cut(s) 529, 551, 553
MwoI GCNNNNNNNGC 4 cut(s) 407, 468, 526, 749
NarI GGCGCC 1 cut(s) 628
NciI CCSGG 6 cut(s) 143, 181, 438, 459, 775, 776
NheI GCTAGC 1 cut(s) 77
NlaIV GGNNCC 6 cut(s) 34, 61, 282, 429, 629, 795
NmeAIII GCCGAG 2 cut(s) 358, 628
NmuCI GTSAC 1 cut(s) 215
NsbI TGCGCA 1 cut(s) 102
NspV TTCGAA 1 cut(s) 505
PciSI GCTCTTC 1 cut(s) 379
PcsI WCGNNNNNNNCGW 3 cut(s) 550, 601, 649
PdmI GAANNNNTTC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 745
PfoI TCCNGGA 1 cut(s) 436
PkrI GCNGC 5 cut(s) 289, 292, 473, 568, 754
PleI GAGTC 2 cut(s) 378, 435
PluTI GGCGCC 1 cut(s) 631
PmeI GTTTAAAC 1 cut(s) 304
PpsI GAGTC 2 cut(s) 378, 435
PspN4I GGNNCC 6 cut(s) 34, 61, 282, 429, 629, 795
PspPI GGNCC 5 cut(s) 32, 242, 280, 575, 793
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 4 cut(s) 188, 588, 838, 874
RsaNI GTAC 4 cut(s) 187, 587, 837, 873
SapI GCTCTTC 1 cut(s) 379
SaqAI TTAA 1 cut(s) 303
SatI GCNGC 5 cut(s) 288, 291, 472, 567, 753
Sau96I GGNCC 5 cut(s) 32, 242, 280, 575, 793
ScaI AGTACT 1 cut(s) 874
SchI GAGTC 2 cut(s) 378, 435
ScrFI CCNGG 6 cut(s) 143, 181, 438, 459, 775, 776
SfoI GGCGCC 1 cut(s) 629
SfuI TTCGAA 1 cut(s) 505
SinI GGWCC 3 cut(s) 32, 280, 793
SmaI CCCGGG 1 cut(s) 776
SsiI CCGC 7 cut(s) 285, 288, 339, 416, 472, 533, 816
SspDI GGCGCC 1 cut(s) 627
SspMI CTAG 4 cut(s) 78, 710, 876, 886
StyD4I CCNGG 6 cut(s) 141, 179, 436, 457, 773, 774
TaaI ACNGT 4 cut(s) 119, 673, 801, 866
TaqI TCGA 2 cut(s) 505, 748
TatI WGTACW 3 cut(s) 586, 836, 872
TauI GCSGC 2 cut(s) 290, 474
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 1 cut(s) 303
Tru9I TTAA 1 cut(s) 303
TscAI CASTG 3 cut(s) 43, 235, 642
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 3 cut(s) 290, 566, 752
Tsp45I GTSAC 1 cut(s) 215
TspMI CCCGGG 1 cut(s) 774
TspRI CASTG 3 cut(s) 43, 235, 642
VpaK11BI GGWCC 3 cut(s) 32, 280, 793
XcmI CCANNNNNNNNNTGG 1 cut(s) 826
XmaI CCCGGG 1 cut(s) 774
XmnI GAANNNNTTC 1 cut(s) 417
XspI CTAG 4 cut(s) 78, 710, 876, 886
ZrmI AGTACT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.