Rmu_sc0000354.1_g000014

synapsis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000354.1
OG0008767
Physical Location & Seq
Reverse (-)
51844 .. 52212
369 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 369 bp
atggcgggcactctggctttgataccaagcaatccgccggtgacgtcatcgtctctgagctccatccgagagcggtgggaggtcgagttcgctcgcttcttcggctaccctccgctcacctccacgtgtccagatctcgtccctcttcctatgaaggtcaggaatcgacggccggtcggtagctggatctccgccgcctccgccgccatgcttcaactcatttatgaccactccagctccgacgtcatcctcaccgtctgctccagcggcaagatcatggtgcctcctcctcctcctctagaaccttctcctttgctttttgaatttttgtcgtcgttgttgttaagctctgacgtttccgactgttga

Protein Analysis

122

Amino Acids

13.19

Weight (kDa)

5.18

Isoelectric Point (pI)

63.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PHS1 PF25349 24 - 93 2e-22 Poor homologous synapsis protein PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 49
AatII GACGTC 2 cut(s) 47, 246
AccB1I GGYRCC 1 cut(s) 280
AccBSI CCGCTC 2 cut(s) 73, 115
AciI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
AclWI GGATC 1 cut(s) 194
AcoI YGGCCR 1 cut(s) 170
AcsI RAATTY 1 cut(s) 323
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 2 cut(s) 44, 243
AflIII ACRYGT 1 cut(s) 125
AgsI TTSAA 2 cut(s) 215, 323
AhdI GACNNNNNGTC 1 cut(s) 173
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 60, 183, 237, 348
AluI AGCT 4 cut(s) 60, 183, 237, 348
Alw21I GWGCWC 1 cut(s) 62
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 1 cut(s) 194
AoxI GGCC 1 cut(s) 170
ApoI RAATTY 1 cut(s) 323
AsuHPI GGTGA 3 cut(s) 52, 109, 244
BaeGI GKGCMC 1 cut(s) 11
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 1 cut(s) 62
BbrPI CACGTG 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 62
BccI CCATC 1 cut(s) 71
BceAI ACGGC 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 299
BglII AGATCT 1 cut(s) 133
BisI GCNGC 3 cut(s) 195, 204, 268
BlsI GCNGC 3 cut(s) 196, 205, 269
BmeRI GACNNNNNGTC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 217, 247
BsaAI YACGTR 1 cut(s) 126
BsaHI GRCGYC 2 cut(s) 44, 243
BsaXI ACNNNNNCTCC 4 cut(s) 71, 101, 221, 251
Bse118I RCCGGY 2 cut(s) 37, 172
BseGI GGATG 2 cut(s) 63, 246
BseMII CTCAG 1 cut(s) 47
BseRI GAGGAG 4 cut(s) 276, 279, 282, 285
BseSI GKGCMC 1 cut(s) 11
BseX3I CGGCCG 1 cut(s) 170
Bsh1285I CGRYCG 2 cut(s) 173, 177
BshFI GGCC 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 280
BsiEI CGRYCG 2 cut(s) 173, 177
BsiHKAI GWGCWC 1 cut(s) 62
BsiSI CCGG 2 cut(s) 38, 173
BslFI GGGAC 1 cut(s) 125
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 1 cut(s) 172
Bsp1286I GDGCHC 2 cut(s) 11, 62
Bsp143I GATC 3 cut(s) 133, 186, 273
BspACI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
BspANI GGCC 1 cut(s) 172
BspCNI CTCAG 1 cut(s) 48
BspLI GGNNCC 1 cut(s) 282
BspPI GGATC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 280
BsrBI CCGCTC 2 cut(s) 73, 115
BsrFI RCCGGY 2 cut(s) 37, 172
BssAI RCCGGY 2 cut(s) 37, 172
BssMI GATC 3 cut(s) 133, 186, 273
BssNI GRCGYC 2 cut(s) 44, 243
Bst4CI ACNGT 2 cut(s) 256, 365
Bst6I CTCTTC 1 cut(s) 150
BstACI GRCGYC 2 cut(s) 44, 243
BstBAI YACGTR 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 7, 94
BstDEI CTNAG 1 cut(s) 56
BstF5I GGATG 2 cut(s) 63, 246
BstKTI GATC 3 cut(s) 136, 189, 276
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 3 cut(s) 133, 186, 273
BstMCI CGRYCG 2 cut(s) 173, 177
BstMWI GCNNNNNNNGC 4 cut(s) 102, 200, 203, 267
BstSLI GKGCMC 1 cut(s) 11
BstX2I RGATCY 2 cut(s) 133, 186
BstYI RGATCY 2 cut(s) 133, 186
BstZI CGGCCG 1 cut(s) 170
BsuRI GGCC 1 cut(s) 172
BtsCI GGATG 2 cut(s) 63, 246
Cac8I GCNNGC 2 cut(s) 7, 94
Cfr10I RCCGGY 2 cut(s) 37, 172
CviAII CATG 2 cut(s) 208, 277
CviJI RGCY 7 cut(s) 17, 60, 105, 172, 183, 237, 348
CviKI_1 RGCY 7 cut(s) 17, 60, 105, 172, 183, 237, 348
DdeI CTNAG 1 cut(s) 56
DpnI GATC 3 cut(s) 135, 188, 275
DpnII GATC 3 cut(s) 133, 186, 273
DrdI GACNNNNNNGTC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 173
DseDI GACNNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 170
EagI CGGCCG 1 cut(s) 170
Eam1104I CTCTTC 1 cut(s) 150
Eam1105I GACNNNNNGTC 1 cut(s) 173
EarI CTCTTC 1 cut(s) 150
EciI GGCGGA 3 cut(s) 24, 181, 190
Ecl136II GAGCTC 1 cut(s) 60
EclXI CGGCCG 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 62
Eco52I CGGCCG 1 cut(s) 170
Eco53kI GAGCTC 1 cut(s) 60
Eco72I CACGTG 1 cut(s) 126
EcoICRI GAGCTC 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 62
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 2 cut(s) 211, 280
FaiI YATR 4 cut(s) 152, 209, 225, 278
FaqI GGGAC 1 cut(s) 125
FatI CATG 2 cut(s) 207, 276
Fnu4HI GCNGC 3 cut(s) 195, 204, 268
FokI GGATG 2 cut(s) 50, 233
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 3 cut(s) 195, 204, 268
FspBI CTAG 1 cut(s) 299
GluI GCNGC 3 cut(s) 195, 204, 268
GsuI CTGGAG 2 cut(s) 217, 247
HaeIII GGCC 1 cut(s) 172
HapII CCGG 2 cut(s) 38, 173
Hin1I GRCGYC 2 cut(s) 44, 243
Hin1II CATG 2 cut(s) 211, 280
HinfI GANTC 1 cut(s) 163
HpaII CCGG 2 cut(s) 38, 173
HphI GGTGA 3 cut(s) 52, 109, 244
Hpy188I TCNGA 5 cut(s) 57, 68, 241, 352, 361
Hpy188III TCNNGA 3 cut(s) 131, 160, 299
Hpy99I CGWCG 3 cut(s) 171, 245, 337
HpyAV CCTTC 2 cut(s) 148, 315
HpyCH4III ACNGT 2 cut(s) 256, 365
HpyCH4IV ACGT 4 cut(s) 44, 125, 243, 354
HpyF10VI GCNNNNNNNGC 4 cut(s) 102, 200, 203, 267
HpyF3I CTNAG 1 cut(s) 56
HpySE526I ACGT 4 cut(s) 44, 125, 243, 354
Hsp92I GRCGYC 2 cut(s) 44, 243
Hsp92II CATG 2 cut(s) 211, 280
Kzo9I GATC 3 cut(s) 133, 186, 273
LmnI GCTCC 3 cut(s) 65, 242, 266
LpnPI CCDG 7 cut(s) 51, 144, 145, 169, 186, 247, 277
MaeI CTAG 1 cut(s) 299
MaeII ACGT 4 cut(s) 44, 125, 243, 354
MaeIII GTNAC 1 cut(s) 40
MalI GATC 3 cut(s) 135, 188, 275
MbiI CCGCTC 2 cut(s) 73, 115
MboI GATC 3 cut(s) 133, 186, 273
MboII GAAGA 2 cut(s) 91, 137
MflI RGATCY 2 cut(s) 133, 186
MhlI GDGCHC 2 cut(s) 11, 62
MluCI AATT 1 cut(s) 323
MmeI TCCRAC 1 cut(s) 264
MseI TTAA 1 cut(s) 344
MspA1I CMGCKG 1 cut(s) 267
MspI CCGG 2 cut(s) 38, 173
MwoI GCNNNNNNNGC 4 cut(s) 102, 200, 203, 267
NdeII GATC 3 cut(s) 133, 186, 273
NlaIII CATG 2 cut(s) 211, 280
NlaIV GGNNCC 1 cut(s) 282
NmuCI GTSAC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 3 cut(s) 196, 205, 269
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
Ppu21I YACGTR 1 cut(s) 126
Psp124BI GAGCTC 1 cut(s) 62
PspCI CACGTG 1 cut(s) 126
PspN4I GGNNCC 1 cut(s) 282
PsuI RGATCY 2 cut(s) 133, 186
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 1 cut(s) 344
SatI GCNGC 3 cut(s) 195, 204, 268
Sau3AI GATC 3 cut(s) 133, 186, 273
SduI GDGCHC 2 cut(s) 11, 62
SgrAI CRCCGGYG 1 cut(s) 37
Sse9I AATT 1 cut(s) 323
SsiI CCGC 9 cut(s) 5, 35, 73, 113, 192, 195, 201, 204, 267
SspMI CTAG 1 cut(s) 299
SstI GAGCTC 1 cut(s) 62
TaaI ACNGT 2 cut(s) 256, 365
TaiI ACGT 4 cut(s) 47, 128, 246, 357
TaqI TCGA 2 cut(s) 84, 166
TasI AATT 1 cut(s) 323
TauI GCSGC 3 cut(s) 197, 206, 270
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TseFI GTSAC 1 cut(s) 40
Tsp45I GTSAC 1 cut(s) 40
TspDTI ATGAA 1 cut(s) 167
XapI RAATTY 1 cut(s) 323
XbaI TCTAGA 1 cut(s) 298
XspI CTAG 1 cut(s) 299
ZraI GACGTC 2 cut(s) 45, 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.