Rmu_sc0000424.1_g000025

ELL-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000424.1
OG0011867
Physical Location & Seq
Forward (+)
106621 .. 109909
3289 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1314 bp
atggccagcaacgccaaggaagagcccaaaaccgctcctcaccccgatcgctggtacaacctaaccctaggcccctccttcaaagacgactcctccagcaagttctgcaccctacgatatgaatttaagccggcctcgattgacaagacgaagccggggtcgctgaagaagacgaaggacaatagggttacggtggagtttcagaacaaccaattggggaagccgaatgtgttgtttaaagggaatagtgaggattataaagacaacgacgccgttttgtttttcgacggccagacgtttcggcttgagaggcttcacagggcggtgaagcagctccggcatgaccgccagcctggggaatccgccgggattcccctggagccgcagctgtctccgccgcccggcaaggtggccaagacgatgcatccttctagaaattcattccctgccgtgccggttgaggtggaacgaattgatattggtgtgccagagaattcaggtgcgaaatctgtgagtaaaggtgtggctgattactcatttgatcaaccaaatgtctctgctgcttcgccagaccataaggatgatgaagttgaggaacatcaagatatagatattgatgacctttttggtagtggatcacctgaagatgggaatgccgctgctgaaaataaatctaatgctgaattcgatatcaatatgcctcatcagaatgagattgatgatgagattgctgatgtagatgttagtggtgatgaagttgataagggcccaaatgctgcagaagcccttcgagcccaggtgaatgcggaggagagtcatgagcagacttcttcttctagtagcagcagtggaagtgggagcagcagtggaagtggtgcaaaatctgtgagtaaaggtgtggctgattactcatttgatcaaccaaatgtctctgctgcttcgccagaccataaggatgatgaagttgaggaacatcaagatatagatattgatgacctttttggtagtggatcacctgaagatgggaatgccgctgctgaaaataaatctaatgctgaatttgatatcaatatgcctcatcagaacgagattgatgatgagattgctgatgtagatgttagtggtgatgaagttgataagggcccaaatgctgcagaagcccttcgagcccaggtgaatgcggaggagagtcatgagcagacttcttcttctagtagtagcagtggaagtgggagcagcagtggaagtggtagcaccagcagcagcagtgacagtgaagccagcgatgatgattctgccgtcaactcaatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EAF

Protein Analysis

437

Amino Acids

46.53

Weight (kDa)

4.36

Isoelectric Point (pI)

48.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EAF PF09816 19 - 118 4.7e-24 RNA polymerase II transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011867)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71080
fragaria_vesca FvH4_4g32210 FvH4_4g32210 FvH4_7g01202
malus_domestica MD13G1027200.v1.1 MD16G1030300.v1.1
prunus_persica Prupe.1G322700_v2.0.a1
pyrus_communis pycom13g02590 pycom16g02580
rosa_chinensis RchiOBHm_Chr4g0440951
rosa_laevigata RLG00000006137
rosa_multiflora Rmu_sc0000424.1_g000025
rosa_roxburghii Rroxscaffold_5G00381810
rosa_rugosa Rorug04G0329600
rosa_samantha Rh4AG381400 Rh4BG394600 Rh4CG409500 Rh4DG387900
rosa_wichuraiana Rw4G032850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 258
AccBSI CCGCTC 1 cut(s) 35
AclWI GGATC 2 cut(s) 643, 1018
AcoI YGGCCR 3 cut(s) 3, 289, 411
AcsI RAATTY 5 cut(s) 122, 436, 493, 683, 1058
AcuI CTGAAG 3 cut(s) 185, 663, 1038
AcyI GRCGYC 1 cut(s) 270
AfaI GTAC 1 cut(s) 56
AfiI CCNNNNNNNGG 5 cut(s) 51, 355, 401, 647, 1022
AgsI TTSAA 1 cut(s) 82
AjnI CCWGG 4 cut(s) 352, 375, 795, 1170
AjuI GAANNNNNNNTTGG 2 cut(s) 197, 229
AluBI AGCT 2 cut(s) 334, 388
AluI AGCT 2 cut(s) 334, 388
Alw26I GTCTC 3 cut(s) 396, 559, 934
AlwI GGATC 2 cut(s) 643, 1018
AoxI GGCC 7 cut(s) 3, 70, 132, 289, 411, 766, 1141
ApaI GGGCCC 2 cut(s) 770, 1145
ApoI RAATTY 5 cut(s) 122, 436, 493, 683, 1058
ArsI GACNNNNNNTTYG 2 cut(s) 143, 175
Asp700I GAANNNNTTC 2 cut(s) 786, 1161
AspA2I CCTAGG 1 cut(s) 67
AspS9I GGNCC 5 cut(s) 71, 766, 767, 1141, 1142
AsuC2I CCSGG 3 cut(s) 156, 367, 402
AsuHPI GGTGA 8 cut(s) 32, 337, 630, 761, 811, 1005, 1136, 1186
AvrII CCTAGG 1 cut(s) 67
BaeGI GKGCMC 2 cut(s) 770, 1145
BalI TGGCCA 2 cut(s) 5, 413
BanII GRGCYC 5 cut(s) 27, 770, 796, 1145, 1171
BbsI GAAGAC 1 cut(s) 176
BccI CCATC 2 cut(s) 641, 1016
BceAI ACGGC 4 cut(s) 257, 304, 434, 1283
BciT130I CCWGG 4 cut(s) 354, 377, 797, 1172
BclI TGATCA 2 cut(s) 541, 916
BcnI CCSGG 3 cut(s) 156, 367, 402
BcoDI GTCTC 3 cut(s) 396, 559, 934
BfaI CTAG 4 cut(s) 68, 432, 837, 1212
BfmI CTRYAG 2 cut(s) 777, 1152
BlnI CCTAGG 1 cut(s) 67
Bme1390I CCNGG 7 cut(s) 156, 354, 367, 377, 402, 797, 1172
BmgT120I GGNCC 5 cut(s) 71, 766, 767, 1141, 1142
BmiI GGNNCC 4 cut(s) 73, 381, 768, 1143
BmrFI CCNGG 7 cut(s) 156, 354, 367, 377, 402, 797, 1172
BmsI GCATC 2 cut(s) 411, 433
BpiI GAAGAC 1 cut(s) 176
BpmI CTGGAG 2 cut(s) 79, 398
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 3 cut(s) 156, 367, 402
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 7 cut(s) 15, 67, 155, 353, 375, 795, 1170
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 850, 880, 1225, 1255
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 355, 401, 647, 1022
Bse118I RCCGGY 2 cut(s) 130, 454
BseBI CCWGG 4 cut(s) 354, 377, 797, 1172
BseDI CCNNGG 7 cut(s) 15, 67, 155, 353, 375, 795, 1170
BseGI GGATG 3 cut(s) 424, 586, 961
BseLI CCNNNNNNNGG 5 cut(s) 51, 355, 401, 647, 1022
BseRI GAGGAG 4 cut(s) 27, 82, 824, 1199
BseSI GKGCMC 2 cut(s) 770, 1145
BsgI GTGCAG 1 cut(s) 91
Bsh1285I CGRYCG 1 cut(s) 49
BshFI GGCC 7 cut(s) 5, 72, 134, 291, 413, 768, 1143
BsiEI CGRYCG 1 cut(s) 49
BsiSI CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
BslI CCNNNNNNNGG 5 cut(s) 51, 355, 401, 647, 1022
BsmAI GTCTC 3 cut(s) 396, 559, 934
BsmI GAATGC 4 cut(s) 658, 808, 1033, 1183
BsnI GGCC 7 cut(s) 5, 72, 134, 291, 413, 768, 1143
Bsp120I GGGCCC 2 cut(s) 766, 1141
Bsp1286I GDGCHC 5 cut(s) 27, 770, 796, 1145, 1171
Bsp143I GATC 5 cut(s) 46, 541, 635, 916, 1010
BspANI GGCC 7 cut(s) 5, 72, 134, 291, 413, 768, 1143
BspHI TCATGA 2 cut(s) 817, 1192
BspLI GGNNCC 4 cut(s) 73, 381, 768, 1143
BspMAI CTGCAG 2 cut(s) 781, 1156
BspPI GGATC 2 cut(s) 643, 1018
BspQI GCTCTTC 1 cut(s) 15
BsrBI CCGCTC 1 cut(s) 35
BsrFI RCCGGY 2 cut(s) 130, 454
BssAI RCCGGY 2 cut(s) 130, 454
BssECI CCNNGG 7 cut(s) 15, 67, 155, 353, 375, 795, 1170
BssMI GATC 5 cut(s) 46, 541, 635, 916, 1010
BssNI GRCGYC 1 cut(s) 270
BssT1I CCWWGG 2 cut(s) 15, 67
Bst2UI CCWGG 4 cut(s) 354, 377, 797, 1172
Bst4CI ACNGT 2 cut(s) 193, 1274
Bst6I CTCTTC 1 cut(s) 15
BstACI GRCGYC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 105
BstC8I GCNNGC 4 cut(s) 7, 132, 350, 1282
BstF5I GGATG 3 cut(s) 424, 586, 961
BstKTI GATC 5 cut(s) 49, 544, 638, 919, 1013
BstMAI GTCTC 3 cut(s) 396, 559, 934
BstMBI GATC 5 cut(s) 46, 541, 635, 916, 1010
BstMCI CGRYCG 1 cut(s) 49
BstNI CCWGG 4 cut(s) 354, 377, 797, 1172
BstSCI CCNGG 7 cut(s) 154, 352, 365, 375, 400, 795, 1170
BstSFI CTRYAG 2 cut(s) 777, 1152
BstSLI GKGCMC 2 cut(s) 770, 1145
BstV2I GAAGAC 1 cut(s) 176
BsuRI GGCC 7 cut(s) 5, 72, 134, 291, 413, 768, 1143
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 3 cut(s) 424, 586, 961
BtsI GCAGTG 5 cut(s) 853, 871, 1228, 1246, 1273
BtsIMutI CAGTG 6 cut(s) 853, 871, 1228, 1246, 1273, 1279
Cac8I GCNNGC 4 cut(s) 7, 132, 350, 1282
CciI TCATGA 2 cut(s) 817, 1192
Cfr10I RCCGGY 2 cut(s) 130, 454
Cfr13I GGNCC 5 cut(s) 71, 766, 767, 1141, 1142
CseI GACGC 1 cut(s) 278
Csp6I GTAC 1 cut(s) 55
CviAII CATG 3 cut(s) 341, 818, 1193
CviQI GTAC 1 cut(s) 55
DpnI GATC 5 cut(s) 48, 543, 637, 918, 1012
DpnII GATC 5 cut(s) 46, 541, 635, 916, 1010
DraI TTTAAA 1 cut(s) 238
EaeI YGGCCR 3 cut(s) 3, 289, 411
Eam1104I CTCTTC 1 cut(s) 15
EarI CTCTTC 1 cut(s) 15
EciI GGCGGA 2 cut(s) 352, 384
Eco130I CCWWGG 2 cut(s) 15, 67
Eco24I GRGCYC 5 cut(s) 27, 770, 796, 1145, 1171
Eco32I GATATC 2 cut(s) 691, 1066
Eco57I CTGAAG 3 cut(s) 185, 663, 1038
EcoO109I RGGNCCY 3 cut(s) 71, 766, 1141
EcoRI GAATTC 2 cut(s) 493, 683
EcoRII CCWGG 4 cut(s) 352, 375, 795, 1170
EcoRV GATATC 2 cut(s) 691, 1066
EcoT14I CCWWGG 2 cut(s) 15, 67
EcoT22I ATGCAT 1 cut(s) 426
EcoT38I GRGCYC 5 cut(s) 27, 770, 796, 1145, 1171
ErhI CCWWGG 2 cut(s) 15, 67
FaeI CATG 3 cut(s) 344, 821, 1196
FatI CATG 3 cut(s) 340, 817, 1192
FbaI TGATCA 2 cut(s) 541, 916
FokI GGATG 3 cut(s) 411, 593, 968
FriOI GRGCYC 5 cut(s) 27, 770, 796, 1145, 1171
FspBI CTAG 4 cut(s) 68, 432, 837, 1212
GsuI CTGGAG 2 cut(s) 79, 398
HaeIII GGCC 7 cut(s) 5, 72, 134, 291, 413, 768, 1143
HapII CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
HgaI GACGC 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 3 cut(s) 344, 821, 1196
HincII GTYRAC 1 cut(s) 1303
HindII GTYRAC 1 cut(s) 1303
HinfI GANTC 6 cut(s) 89, 359, 370, 814, 1189, 1292
HpaII CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
HphI GGTGA 8 cut(s) 32, 337, 630, 761, 811, 1005, 1136, 1186
Hpy166II GTNNAC 1 cut(s) 1303
Hpy188I TCNGA 4 cut(s) 204, 708, 1083, 1313
Hpy188III TCNNGA 5 cut(s) 432, 602, 818, 977, 1193
Hpy8I GTNNAC 1 cut(s) 1303
Hpy99I CGWCG 2 cut(s) 272, 290
HpyAV CCTTC 5 cut(s) 88, 169, 438, 797, 1172
HpyCH4III ACNGT 2 cut(s) 193, 1274
HpyCH4IV ACGT 1 cut(s) 296
HpyCH4V TGCA 5 cut(s) 108, 424, 779, 878, 1154
HpySE526I ACGT 1 cut(s) 296
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 3 cut(s) 344, 821, 1196
KroI GCCGGC 1 cut(s) 130
KroNI GCCGGC 1 cut(s) 132
Ksp22I TGATCA 2 cut(s) 541, 916
Kzo9I GATC 5 cut(s) 46, 541, 635, 916, 1010
LguI GCTCTTC 1 cut(s) 15
LmnI GCTCC 5 cut(s) 40, 339, 379, 858, 1233
LweI GCATC 2 cut(s) 411, 433
MaeI CTAG 4 cut(s) 68, 432, 837, 1212
MaeII ACGT 1 cut(s) 296
MaeIII GTNAC 2 cut(s) 187, 1268
MalI GATC 5 cut(s) 48, 543, 637, 918, 1012
MbiI CCGCTC 1 cut(s) 35
MboI GATC 5 cut(s) 46, 541, 635, 916, 1010
MboII GAAGA 9 cut(s) 32, 178, 181, 656, 822, 825, 1031, 1197, 1200
MfeI CAATTG 1 cut(s) 212
MhlI GDGCHC 5 cut(s) 27, 770, 796, 1145, 1171
MlsI TGGCCA 2 cut(s) 5, 413
MluCI AATT 7 cut(s) 122, 212, 436, 471, 493, 683, 1058
MluNI TGGCCA 2 cut(s) 5, 413
MlyI GAGTC 3 cut(s) 83, 823, 1198
Mox20I TGGCCA 2 cut(s) 5, 413
Mph1103I ATGCAT 1 cut(s) 426
MroNI GCCGGC 1 cut(s) 130
MroXI GAANNNNTTC 2 cut(s) 786, 1161
MscI TGGCCA 2 cut(s) 5, 413
MseI TTAA 2 cut(s) 126, 237
MslI CAYNNNNRTG 3 cut(s) 579, 708, 954
Msp20I TGGCCA 2 cut(s) 5, 413
MspA1I CMGCKG 3 cut(s) 388, 659, 1034
MspI CCGG 6 cut(s) 131, 155, 337, 366, 402, 455
MspR9I CCNGG 7 cut(s) 156, 354, 367, 377, 402, 797, 1172
MunI CAATTG 1 cut(s) 212
Mva1269I GAATGC 4 cut(s) 658, 808, 1033, 1183
MvaI CCWGG 4 cut(s) 354, 377, 797, 1172
NaeI GCCGGC 1 cut(s) 132
NciI CCSGG 3 cut(s) 156, 367, 402
NdeII GATC 5 cut(s) 46, 541, 635, 916, 1010
NgoMIV GCCGGC 1 cut(s) 130
NlaIII CATG 3 cut(s) 344, 821, 1196
NlaIV GGNNCC 4 cut(s) 73, 381, 768, 1143
NmuCI GTSAC 1 cut(s) 1268
NsiI ATGCAT 1 cut(s) 426
PagI TCATGA 2 cut(s) 817, 1192
PciSI GCTCTTC 1 cut(s) 15
PctI GAATGC 4 cut(s) 658, 808, 1033, 1183
PdiI GCCGGC 1 cut(s) 132
PdmI GAANNNNTTC 2 cut(s) 786, 1161
PfeI GAWTC 3 cut(s) 359, 370, 1292
Ple19I CGATCG 1 cut(s) 49
PleI GAGTC 3 cut(s) 83, 822, 1197
PpsI GAGTC 3 cut(s) 83, 822, 1197
PsiI TTATAA 1 cut(s) 258
Psp6I CCWGG 4 cut(s) 352, 375, 795, 1170
PspGI CCWGG 4 cut(s) 352, 375, 795, 1170
PspN4I GGNNCC 4 cut(s) 73, 381, 768, 1143
PspOMI GGGCCC 2 cut(s) 766, 1141
PspPI GGNCC 5 cut(s) 71, 766, 767, 1141, 1142
PstI CTGCAG 2 cut(s) 781, 1156
PvuI CGATCG 1 cut(s) 49
PvuII CAGCTG 1 cut(s) 388
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
RseI CAYNNNNRTG 3 cut(s) 579, 708, 954
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 126, 237
Sau3AI GATC 5 cut(s) 46, 541, 635, 916, 1010
Sau96I GGNCC 5 cut(s) 71, 766, 767, 1141, 1142
SchI GAGTC 3 cut(s) 83, 823, 1198
ScrFI CCNGG 7 cut(s) 156, 354, 367, 377, 402, 797, 1172
SduI GDGCHC 5 cut(s) 27, 770, 796, 1145, 1171
SfaNI GCATC 2 cut(s) 411, 433
SfcI CTRYAG 2 cut(s) 777, 1152
SmiMI CAYNNNNRTG 3 cut(s) 579, 708, 954
SmlI CTYRAG 1 cut(s) 305
SmoI CTYRAG 1 cut(s) 305
Sse9I AATT 7 cut(s) 122, 212, 436, 471, 493, 683, 1058
SspMI CTAG 4 cut(s) 68, 432, 837, 1212
StyD4I CCNGG 7 cut(s) 154, 352, 365, 375, 400, 795, 1170
StyI CCWWGG 2 cut(s) 15, 67
TaaI ACNGT 2 cut(s) 193, 1274
TaiI ACGT 1 cut(s) 299
TaqI TCGA 5 cut(s) 137, 285, 687, 790, 1165
TasI AATT 7 cut(s) 122, 212, 436, 471, 493, 683, 1058
TauI GCSGC 4 cut(s) 385, 400, 659, 1034
TfiI GAWTC 3 cut(s) 359, 370, 1292
Tru1I TTAA 2 cut(s) 126, 237
Tru9I TTAA 2 cut(s) 126, 237
TscAI CASTG 6 cut(s) 853, 871, 1228, 1246, 1273, 1279
TseFI GTSAC 1 cut(s) 1268
Tsp45I GTSAC 1 cut(s) 1268
TspDTI ATGAA 6 cut(s) 135, 429, 600, 768, 975, 1143
TspRI CASTG 6 cut(s) 853, 871, 1228, 1246, 1273, 1279
XapI RAATTY 5 cut(s) 122, 436, 493, 683, 1058
XbaI TCTAGA 1 cut(s) 431
XmaJI CCTAGG 1 cut(s) 67
XmnI GAANNNNTTC 2 cut(s) 786, 1161
XspI CTAG 4 cut(s) 68, 432, 837, 1212
Zsp2I ATGCAT 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.