Rmu_sc0000497.1_g000001

Ammonium transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000497.1
OG0017022
Physical Location & Seq
Reverse (-)
1595 .. 1903
309 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
atgctcggatccggagtcatagacttcaccgactccggtgtcgtctacatggtcggaggatgcatcgttgcactgacgactctcttcgggaaacgaatcttctccggccactggaacgccaccgacgtctacaacggcctcctcaacggcttctctgccatcatcttcggctgctccgtcgtcgagccttgggctgccatcatctgcagcttcatttccaccaccatcttagtctcctgcaacaaattcgccgagaaggtcaggtacgactaccccttggaggcggctcagaaattgggcttgagatga

Protein Analysis

102

Amino Acids

11.02

Weight (kDa)

6.52

Isoelectric Point (pI)

24.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ammonium_transp PF00909 20 - 96 1.8e-12 Ammonium Transporter Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AatII GACGTC 1 cut(s) 129
AccI GTMKAC 2 cut(s) 45, 129
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 1 cut(s) 284
AclWI GGATC 2 cut(s) 3, 16
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 1 cut(s) 245
AcyI GRCGYC 1 cut(s) 126
AfaI GTAC 1 cut(s) 266
AfiI CCNNNNNNNGG 2 cut(s) 111, 280
AluBI AGCT 1 cut(s) 210
AluI AGCT 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 238
AlwI GGATC 2 cut(s) 3, 16
Aor13HI TCCGGA 1 cut(s) 11
AoxI GGCC 2 cut(s) 106, 136
ApeKI GCWGC 3 cut(s) 171, 194, 207
ApoI RAATTY 1 cut(s) 245
AsuHPI GGTGA 1 cut(s) 19
BamHI GGATCC 1 cut(s) 8
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbvI GCAGC 3 cut(s) 158, 181, 219
BccI CCATC 3 cut(s) 167, 206, 233
BceAI ACGGC 2 cut(s) 151, 163
BcgI CGANNNNNNTGC 2 cut(s) 229, 263
BcoDI GTCTC 1 cut(s) 238
BfmI CTRYAG 1 cut(s) 205
BisI GCNGC 4 cut(s) 172, 195, 208, 285
BlsI GCNGC 4 cut(s) 173, 196, 209, 286
BmiI GGNNCC 1 cut(s) 10
BmsI GCATC 2 cut(s) 50, 72
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 2 cut(s) 188, 276
BsaWI WCCGGW 2 cut(s) 11, 35
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 280
Bse1I ACTGG 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 11
BseDI CCNNGG 2 cut(s) 188, 276
BseGI GGATG 1 cut(s) 65
BseLI CCNNNNNNNGG 2 cut(s) 111, 280
BseMII CTCAG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 116
BseRI GAGGAG 1 cut(s) 131
BseXI GCAGC 3 cut(s) 158, 181, 219
BshFI GGCC 2 cut(s) 108, 138
BsiSI CCGG 3 cut(s) 12, 36, 105
BslI CCNNNNNNNGG 2 cut(s) 111, 280
BsmAI GTCTC 1 cut(s) 238
BsnI GGCC 2 cut(s) 108, 138
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 1 cut(s) 8
BspACI CCGC 1 cut(s) 284
BspANI GGCC 2 cut(s) 108, 138
BspCNI CTCAG 1 cut(s) 301
BspEI TCCGGA 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 10
BspMAI CTGCAG 1 cut(s) 209
BspPI GGATC 2 cut(s) 3, 16
BsrI ACTGG 1 cut(s) 116
BssECI CCNNGG 2 cut(s) 188, 276
BssMI GATC 1 cut(s) 8
BssNI GRCGYC 1 cut(s) 126
BssT1I CCWWGG 2 cut(s) 188, 276
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 1 cut(s) 126
BstDEI CTNAG 2 cut(s) 229, 288
BstF5I GGATG 1 cut(s) 65
BstKTI GATC 1 cut(s) 11
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 8
BstSFI CTRYAG 1 cut(s) 205
BstV1I GCAGC 3 cut(s) 158, 181, 219
BstX2I RGATCY 1 cut(s) 8
BstYI RGATCY 1 cut(s) 8
BsuRI GGCC 2 cut(s) 108, 138
BtsCI GGATG 1 cut(s) 65
BtsIMutI CAGTG 2 cut(s) 71, 109
Csp6I GTAC 1 cut(s) 265
CviAII CATG 1 cut(s) 49
CviJI RGCY 9 cut(s) 108, 138, 150, 171, 187, 194, 210, 287, 300
CviKI_1 RGCY 9 cut(s) 108, 138, 150, 171, 187, 194, 210, 287, 300
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 2 cut(s) 229, 288
DpnI GATC 1 cut(s) 10
DpnII GATC 1 cut(s) 8
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco130I CCWWGG 2 cut(s) 188, 276
EcoT14I CCWWGG 2 cut(s) 188, 276
EcoT22I ATGCAT 1 cut(s) 65
ErhI CCWWGG 2 cut(s) 188, 276
FaeI CATG 1 cut(s) 52
FaiI YATR 2 cut(s) 20, 50
FatI CATG 1 cut(s) 48
FblI GTMKAC 2 cut(s) 45, 129
Fnu4HI GCNGC 4 cut(s) 172, 195, 208, 285
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 172, 195, 208, 285
GluI GCNGC 4 cut(s) 172, 195, 208, 285
HaeIII GGCC 2 cut(s) 108, 138
HapII CCGG 3 cut(s) 12, 36, 105
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 52
HinfI GANTC 4 cut(s) 15, 32, 79, 96
HpaII CCGG 3 cut(s) 12, 36, 105
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 2 cut(s) 46, 130
Hpy188I TCNGA 3 cut(s) 8, 56, 291
Hpy188III TCNNGA 2 cut(s) 12, 88
Hpy8I GTNNAC 2 cut(s) 46, 130
Hpy99I CGWCG 3 cut(s) 128, 182, 185
HpyAV CCTTC 1 cut(s) 250
HpyCH4IV ACGT 1 cut(s) 126
HpyCH4V TGCA 4 cut(s) 63, 71, 207, 240
HpyF3I CTNAG 2 cut(s) 229, 288
HpySE526I ACGT 1 cut(s) 126
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 52
Kpn2I TCCGGA 1 cut(s) 11
Kzo9I GATC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 179
LpnPI CCDG 6 cut(s) 25, 49, 97, 118, 247, 250
Lsp1109I GCAGC 3 cut(s) 158, 181, 219
LweI GCATC 2 cut(s) 50, 72
MaeII ACGT 1 cut(s) 126
MalI GATC 1 cut(s) 10
MboI GATC 1 cut(s) 8
MboII GAAGA 3 cut(s) 76, 91, 157
MflI RGATCY 1 cut(s) 8
MluCI AATT 2 cut(s) 245, 293
MlyI GAGTC 3 cut(s) 24, 26, 73
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 4 cut(s) 50, 149, 152, 274
Mph1103I ATGCAT 1 cut(s) 65
MroI TCCGGA 1 cut(s) 11
MspI CCGG 3 cut(s) 12, 36, 105
NdeII GATC 1 cut(s) 8
NlaIII CATG 1 cut(s) 52
NlaIV GGNNCC 1 cut(s) 10
NmeAIII GCCGAG 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 65
PcsI WCGNNNNNNNCGW 2 cut(s) 123, 174
PfeI GAWTC 1 cut(s) 96
PkrI GCNGC 4 cut(s) 173, 196, 209, 286
PleI GAGTC 3 cut(s) 23, 26, 73
PpsI GAGTC 3 cut(s) 23, 26, 73
PspN4I GGNNCC 1 cut(s) 10
PstI CTGCAG 1 cut(s) 209
PsuI RGATCY 1 cut(s) 8
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SatI GCNGC 4 cut(s) 172, 195, 208, 285
Sau3AI GATC 1 cut(s) 8
SchI GAGTC 3 cut(s) 24, 26, 73
SetI ASST 4 cut(s) 129, 212, 261, 266
SfaNI GCATC 2 cut(s) 50, 72
SfcI CTRYAG 1 cut(s) 205
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 2 cut(s) 245, 293
SsiI CCGC 1 cut(s) 284
StyI CCWWGG 2 cut(s) 188, 276
TaiI ACGT 1 cut(s) 129
TaqI TCGA 1 cut(s) 183
TasI AATT 2 cut(s) 245, 293
TauI GCSGC 1 cut(s) 287
TfiI GAWTC 1 cut(s) 96
TscAI CASTG 2 cut(s) 78, 116
TseI GCWGC 3 cut(s) 171, 194, 207
TspDTI ATGAA 1 cut(s) 202
TspGWI ACGGA 1 cut(s) 166
TspRI CASTG 2 cut(s) 78, 116
XapI RAATTY 1 cut(s) 245
XmiI GTMKAC 2 cut(s) 45, 129
ZraI GACGTC 1 cut(s) 127
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.