Rmu_sc0000539.1_g000094

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0004370
Physical Location & Seq
Forward (+)
427143 .. 430422
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1089 bp
atgattctctccggtcgcgaagtaattcttggggactgcacatgccgttggtttgtcccgacggcgccgtcgtcgcctatgcttggaaacggcagctcgccggccaggccggcgcgtccgcagtggacaggaccaggaaggcagctagaagagtctggagttctgaatgattctctccggtcgcgaagtaattcttggggactgcacatgccgttggtttgtcccgacggcgccgtcgtcgcctatgcttggaaacggcagctcgccggccaggccggcgcgtccgcagtggacaggaccaggttagctctgaaggccttcactgatcagaaaagaagattttttcctcaccttgatgatggatcggacagtcagagtgacgaaccagtttcaaagaaaccgtgtgtttccccatcattgactacacacaatcaagaagagctactagattgtaagacactatcagaggttttaatgagcttggaaaagaaggtgcctaacttgaaagttttcacctacgaacgattggattggttaaaaagagcatcgtcactgccagctaatgagaatccactggaaacagtgaaggaccatagttttcatagttcgagtaagttacgagctactccactggctgatgttgcgatagataaggttgctgtcatcgagttgctgtttccgtctgtcttcagagctgtagtatcattgcatcctgctggttctattgacccagacgcagtagctttcttttctccagaagagggaggcagctacattcatgcaagaggtttttcagtttatcatgtgtttaaacatatcacggaacatgctggtttggccttgcagtactttcttggagttcaagctgaaacagctctatattcccttttgctctggatctgcagctaccaaactctatttacaaaagtttgcagtaagtgcgaacgactgctagctatggaccgacaatcagctttactgttgcctccggttaatcgtccttaccggcagttctccacatcaaatatatcctccagtttaaatcttaccgagacttaccatgtaggttgttttacggaggaaaggtga

Protein Analysis

362

Amino Acids

40.1

Weight (kDa)

7.62

Isoelectric Point (pI)

57.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med27 PF11571 265 - 357 5.8e-19 Mediator complex subunit 27
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 67, 233
AccB1I GGYRCC 3 cut(s) 64, 230, 493
AccII CGCG 4 cut(s) 18, 115, 184, 281
AciI CCGC 2 cut(s) 119, 285
AclWI GGATC 2 cut(s) 370, 905
AcoI YGGCCR 2 cut(s) 102, 268
AcuI CTGAAG 2 cut(s) 332, 673
AcyI GRCGYC 2 cut(s) 65, 231
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 3 cut(s) 83, 249, 761
AgsI TTSAA 3 cut(s) 393, 505, 863
AjnI CCWGG 4 cut(s) 104, 133, 270, 299
AjuI GAANNNNNNNTTGG 4 cut(s) 12, 44, 178, 210
Alw26I GTCTC 1 cut(s) 1046
AlwI GGATC 2 cut(s) 370, 905
AoxI GGCC 6 cut(s) 102, 107, 268, 273, 315, 837
ApeKI GCWGC 5 cut(s) 93, 142, 259, 768, 903
Asp700I GAANNNNTTC 4 cut(s) 24, 190, 317, 509
AspLEI GCGC 4 cut(s) 67, 115, 233, 281
AspS9I GGNCC 4 cut(s) 131, 297, 589, 961
AsuHPI GGTGA 2 cut(s) 341, 505
AsuNHI GCTAGC 1 cut(s) 952
AvaII GGWCC 4 cut(s) 131, 297, 589, 961
BanI GGYRCC 3 cut(s) 64, 230, 493
BbsI GAAGAC 1 cut(s) 679
BbvI GCAGC 5 cut(s) 105, 154, 271, 780, 915
BccI CCATC 2 cut(s) 353, 421
BceAI ACGGC 8 cut(s) 30, 52, 78, 106, 196, 218, 244, 272
BciT130I CCWGG 4 cut(s) 106, 135, 272, 301
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 1046
BfaI CTAG 3 cut(s) 146, 446, 953
BfmI CTRYAG 2 cut(s) 696, 901
BfoI RGCGCY 2 cut(s) 68, 234
BglI GCCNNNNNGGC 4 cut(s) 106, 110, 272, 276
BisI GCNGC 5 cut(s) 94, 143, 260, 769, 904
BlsI GCNGC 5 cut(s) 95, 144, 261, 770, 905
BmcAI AGTACT 1 cut(s) 848
Bme1390I CCNGG 4 cut(s) 106, 135, 272, 301
Bme18I GGWCC 4 cut(s) 131, 297, 589, 961
BmgT120I GGNCC 4 cut(s) 131, 297, 589, 961
BmiI GGNNCC 3 cut(s) 66, 232, 495
BmrFI CCNGG 4 cut(s) 106, 135, 272, 301
BmsI GCATC 2 cut(s) 554, 718
BmtI GCTAGC 1 cut(s) 956
BpiI GAAGAC 1 cut(s) 679
BpmI CTGGAG 3 cut(s) 177, 738, 1018
BsaHI GRCGYC 2 cut(s) 65, 231
BsaWI WCCGGW 3 cut(s) 11, 177, 988
BsaXI ACNNNNNCTCC 1 cut(s) 1070
Bsc4I CCNNNNNNNGG 3 cut(s) 83, 249, 761
Bse118I RCCGGY 5 cut(s) 100, 109, 266, 275, 1005
Bse1I ACTGG 4 cut(s) 386, 579, 636, 1035
Bse3DI GCAATG 1 cut(s) 704
BseBI CCWGG 4 cut(s) 106, 135, 272, 301
BseGI GGATG 1 cut(s) 709
BseLI CCNNNNNNNGG 3 cut(s) 83, 249, 761
BseMI GCAATG 1 cut(s) 704
BseNI ACTGG 4 cut(s) 386, 579, 636, 1035
BseXI GCAGC 5 cut(s) 105, 154, 271, 780, 915
BsgI GTGCAG 2 cut(s) 22, 188
Bsh1236I CGCG 4 cut(s) 18, 115, 184, 281
Bsh1285I CGRYCG 2 cut(s) 16, 182
BshFI GGCC 6 cut(s) 104, 109, 270, 275, 317, 839
BshNI GGYRCC 3 cut(s) 64, 230, 493
BsiEI CGRYCG 2 cut(s) 16, 182
BsiSI CCGG 8 cut(s) 12, 101, 110, 178, 267, 276, 989, 1006
BslFI GGGAC 4 cut(s) 41, 47, 207, 213
BslI CCNNNNNNNGG 3 cut(s) 83, 249, 761
BsmAI GTCTC 1 cut(s) 1046
BsmFI GGGAC 4 cut(s) 41, 47, 207, 213
BsnI GGCC 6 cut(s) 104, 109, 270, 275, 317, 839
Bsp143I GATC 3 cut(s) 325, 362, 897
Bsp68I TCGCGA 2 cut(s) 18, 184
BspACI CCGC 2 cut(s) 119, 285
BspANI GGCC 6 cut(s) 104, 109, 270, 275, 317, 839
BspFNI CGCG 4 cut(s) 18, 115, 184, 281
BspLI GGNNCC 3 cut(s) 66, 232, 495
BspMAI CTGCAG 1 cut(s) 905
BspOI GCTAGC 1 cut(s) 956
BspPI GGATC 2 cut(s) 370, 905
BspQI GCTCTTC 1 cut(s) 432
BspT107I GGYRCC 3 cut(s) 64, 230, 493
BsrDI GCAATG 1 cut(s) 704
BsrFI RCCGGY 5 cut(s) 100, 109, 266, 275, 1005
BsrI ACTGG 4 cut(s) 386, 579, 636, 1035
BssAI RCCGGY 5 cut(s) 100, 109, 266, 275, 1005
BssMI GATC 3 cut(s) 325, 362, 897
BssNI GRCGYC 2 cut(s) 65, 231
Bst2UI CCWGG 4 cut(s) 106, 135, 272, 301
Bst4CI ACNGT 4 cut(s) 371, 402, 583, 981
Bst6I CTCTTC 3 cut(s) 144, 432, 753
BstACI GRCGYC 2 cut(s) 65, 231
BstAPI GCANNNNNTGC 1 cut(s) 939
BstC8I GCNNGC 8 cut(s) 98, 102, 111, 264, 268, 277, 558, 954
BstF5I GGATG 1 cut(s) 709
BstFNI CGCG 4 cut(s) 18, 115, 184, 281
BstH2I RGCGCY 2 cut(s) 68, 234
BstHHI GCGC 4 cut(s) 67, 115, 233, 281
BstKTI GATC 3 cut(s) 328, 365, 900
BstMAI GTCTC 1 cut(s) 1046
BstMBI GATC 3 cut(s) 325, 362, 897
BstMCI CGRYCG 2 cut(s) 16, 182
BstNI CCWGG 4 cut(s) 106, 135, 272, 301
BstNSI RCATGY 3 cut(s) 45, 211, 830
BstSCI CCNGG 4 cut(s) 104, 133, 270, 299
BstSFI CTRYAG 2 cut(s) 696, 901
BstUI CGCG 4 cut(s) 18, 115, 184, 281
BstV1I GCAGC 5 cut(s) 105, 154, 271, 780, 915
BstV2I GAAGAC 1 cut(s) 679
BstX2I RGATCY 1 cut(s) 897
BstYI RGATCY 1 cut(s) 897
BsuRI GGCC 6 cut(s) 104, 109, 270, 275, 317, 839
BtsCI GGATG 1 cut(s) 709
BtsI GCAGTG 3 cut(s) 128, 294, 551
BtsIMutI CAGTG 7 cut(s) 128, 294, 321, 551, 572, 588, 629
BtuMI TCGCGA 2 cut(s) 18, 184
Cac8I GCNNGC 8 cut(s) 98, 102, 111, 264, 268, 277, 558, 954
CfoI GCGC 4 cut(s) 67, 115, 233, 281
Cfr10I RCCGGY 5 cut(s) 100, 109, 266, 275, 1005
Cfr13I GGNCC 4 cut(s) 131, 297, 589, 961
CseI GACGC 3 cut(s) 104, 270, 743
CsiI ACCWGGT 1 cut(s) 299
Csp6I GTAC 1 cut(s) 847
CviAII CATG 6 cut(s) 42, 208, 779, 803, 827, 1061
CviQI GTAC 1 cut(s) 847
DinI GGCGCC 2 cut(s) 66, 232
DpnI GATC 3 cut(s) 327, 364, 899
DpnII GATC 3 cut(s) 325, 362, 897
DraI TTTAAA 2 cut(s) 811, 1041
DrdI GACNNNNNNGTC 2 cut(s) 67, 233
DseDI GACNNNNNNGTC 2 cut(s) 67, 233
EaeI YGGCCR 2 cut(s) 102, 268
Eam1104I CTCTTC 3 cut(s) 144, 432, 753
EarI CTCTTC 3 cut(s) 144, 432, 753
Eco147I AGGCCT 1 cut(s) 317
Eco47I GGWCC 4 cut(s) 131, 297, 589, 961
Eco57I CTGAAG 2 cut(s) 332, 673
EcoRII CCWGG 4 cut(s) 104, 133, 270, 299
EgeI GGCGCC 2 cut(s) 66, 232
EheI GGCGCC 2 cut(s) 66, 232
FaeI CATG 6 cut(s) 45, 211, 782, 806, 830, 1064
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 178, 210
FaqI GGGAC 4 cut(s) 41, 47, 207, 213
FatI CATG 6 cut(s) 41, 207, 778, 802, 826, 1060
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 5 cut(s) 94, 143, 260, 769, 904
FokI GGATG 1 cut(s) 696
Fsp4HI GCNGC 5 cut(s) 94, 143, 260, 769, 904
FspBI CTAG 3 cut(s) 146, 446, 953
GlaI GCGC 4 cut(s) 66, 114, 232, 280
GluI GCNGC 5 cut(s) 94, 143, 260, 769, 904
GsuI CTGGAG 3 cut(s) 177, 738, 1018
HaeII RGCGCY 2 cut(s) 68, 234
HaeIII GGCC 6 cut(s) 104, 109, 270, 275, 317, 839
HapII CCGG 8 cut(s) 12, 101, 110, 178, 267, 276, 989, 1006
HgaI GACGC 3 cut(s) 104, 270, 743
HhaI GCGC 4 cut(s) 67, 115, 233, 281
Hin1I GRCGYC 2 cut(s) 65, 231
Hin1II CATG 6 cut(s) 45, 211, 782, 806, 830, 1064
Hin6I GCGC 4 cut(s) 65, 113, 231, 279
HinP1I GCGC 4 cut(s) 65, 113, 231, 279
HinfI GANTC 4 cut(s) 4, 152, 170, 568
HpaII CCGG 8 cut(s) 12, 101, 110, 178, 267, 276, 989, 1006
HphI GGTGA 2 cut(s) 341, 505
Hpy166II GTNNAC 2 cut(s) 126, 292
Hpy188I TCNGA 7 cut(s) 165, 312, 330, 367, 375, 466, 692
Hpy188III TCNNGA 8 cut(s) 17, 58, 156, 183, 224, 434, 755, 895
Hpy8I GTNNAC 2 cut(s) 126, 292
Hpy99I CGWCG 6 cut(s) 64, 73, 76, 230, 239, 242
HpyAV CCTTC 5 cut(s) 132, 307, 328, 484, 580
HpyCH4III ACNGT 4 cut(s) 371, 402, 583, 981
HpyCH4V TGCA 7 cut(s) 39, 205, 709, 782, 844, 903, 933
Hsp92I GRCGYC 2 cut(s) 65, 231
Hsp92II CATG 6 cut(s) 45, 211, 782, 806, 830, 1064
HspAI GCGC 4 cut(s) 65, 113, 231, 279
KasI GGCGCC 2 cut(s) 64, 230
KroI GCCGGC 4 cut(s) 100, 109, 266, 275
KroNI GCCGGC 4 cut(s) 102, 111, 268, 277
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 3 cut(s) 325, 362, 897
LguI GCTCTTC 1 cut(s) 432
Lsp1109I GCAGC 5 cut(s) 105, 154, 271, 780, 915
LweI GCATC 2 cut(s) 554, 718
MabI ACCWGGT 1 cut(s) 299
MaeI CTAG 3 cut(s) 146, 446, 953
MaeIII GTNAC 3 cut(s) 377, 549, 615
MalI GATC 3 cut(s) 327, 364, 899
MboI GATC 3 cut(s) 325, 362, 897
MboII GAAGA 5 cut(s) 161, 348, 449, 679, 770
MflI RGATCY 1 cut(s) 897
MluCI AATT 2 cut(s) 24, 190
Mly113I GGCGCC 2 cut(s) 65, 231
MlyI GAGTC 1 cut(s) 161
MnlI CCTC 8 cut(s) 357, 460, 754, 758, 779, 996, 1042, 1072
MroNI GCCGGC 4 cut(s) 100, 109, 266, 275
MroXI GAANNNNTTC 4 cut(s) 24, 190, 317, 509
MseI TTAA 5 cut(s) 473, 536, 810, 993, 1040
MslI CAYNNNNRTG 1 cut(s) 354
MspI CCGG 8 cut(s) 12, 101, 110, 178, 267, 276, 989, 1006
MspR9I CCNGG 4 cut(s) 106, 135, 272, 301
MssI GTTTAAAC 1 cut(s) 811
MvaI CCWGG 4 cut(s) 106, 135, 272, 301
MvnI CGCG 4 cut(s) 18, 115, 184, 281
NaeI GCCGGC 4 cut(s) 102, 111, 268, 277
NarI GGCGCC 2 cut(s) 65, 231
NdeII GATC 3 cut(s) 325, 362, 897
NgoMIV GCCGGC 4 cut(s) 100, 109, 266, 275
NheI GCTAGC 1 cut(s) 952
NlaIII CATG 6 cut(s) 45, 211, 782, 806, 830, 1064
NlaIV GGNNCC 3 cut(s) 66, 232, 495
NmuCI GTSAC 2 cut(s) 377, 549
NruI TCGCGA 2 cut(s) 18, 184
NspI RCATGY 3 cut(s) 45, 211, 830
PceI AGGCCT 1 cut(s) 317
PciSI GCTCTTC 1 cut(s) 432
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 234
PdiI GCCGGC 4 cut(s) 102, 111, 268, 277
PdmI GAANNNNTTC 4 cut(s) 24, 190, 317, 509
PfeI GAWTC 3 cut(s) 4, 170, 568
PkrI GCNGC 5 cut(s) 95, 144, 261, 770, 905
PleI GAGTC 1 cut(s) 160
PluTI GGCGCC 2 cut(s) 68, 234
PmeI GTTTAAAC 1 cut(s) 811
PpsI GAGTC 1 cut(s) 160
Psp6I CCWGG 4 cut(s) 104, 133, 270, 299
PspGI CCWGG 4 cut(s) 104, 133, 270, 299
PspN4I GGNNCC 3 cut(s) 66, 232, 495
PspPI GGNCC 4 cut(s) 131, 297, 589, 961
PstI CTGCAG 1 cut(s) 905
PsuI RGATCY 1 cut(s) 897
RruI TCGCGA 2 cut(s) 18, 184
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 1 cut(s) 354
SapI GCTCTTC 1 cut(s) 432
SaqAI TTAA 5 cut(s) 473, 536, 810, 993, 1040
SatI GCNGC 5 cut(s) 94, 143, 260, 769, 904
Sau3AI GATC 3 cut(s) 325, 362, 897
Sau96I GGNCC 4 cut(s) 131, 297, 589, 961
ScaI AGTACT 1 cut(s) 848
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 4 cut(s) 106, 135, 272, 301
SexAI ACCWGGT 1 cut(s) 299
SfaNI GCATC 2 cut(s) 554, 718
SfcI CTRYAG 2 cut(s) 696, 901
SfoI GGCGCC 2 cut(s) 66, 232
SinI GGWCC 4 cut(s) 131, 297, 589, 961
SmiMI CAYNNNNRTG 1 cut(s) 354
Sse9I AATT 2 cut(s) 24, 190
SseBI AGGCCT 1 cut(s) 317
SsiI CCGC 2 cut(s) 119, 285
SspDI GGCGCC 2 cut(s) 64, 230
SspMI CTAG 3 cut(s) 146, 446, 953
StuI AGGCCT 1 cut(s) 317
StyD4I CCNGG 4 cut(s) 104, 133, 270, 299
TaaI ACNGT 4 cut(s) 371, 402, 583, 981
TaqI TCGA 2 cut(s) 608, 666
TaqII GACCGA 1 cut(s) 978
TasI AATT 2 cut(s) 24, 190
TatI WGTACW 1 cut(s) 846
TfiI GAWTC 3 cut(s) 4, 170, 568
Tru1I TTAA 5 cut(s) 473, 536, 810, 993, 1040
Tru9I TTAA 5 cut(s) 473, 536, 810, 993, 1040
TscAI CASTG 7 cut(s) 128, 294, 328, 558, 579, 588, 636
TseFI GTSAC 2 cut(s) 377, 549
TseI GCWGC 5 cut(s) 93, 142, 259, 768, 903
Tsp45I GTSAC 2 cut(s) 377, 549
TspDTI ATGAA 2 cut(s) 590, 767
TspGWI ACGGA 2 cut(s) 669, 836
TspRI CASTG 7 cut(s) 128, 294, 328, 558, 579, 588, 636
VpaK11BI GGWCC 4 cut(s) 131, 297, 589, 961
XceI RCATGY 3 cut(s) 45, 211, 830
XmnI GAANNNNTTC 4 cut(s) 24, 190, 317, 509
XspI CTAG 3 cut(s) 146, 446, 953
ZrmI AGTACT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.