Rmu_sc0000580.1_g000113

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000580.1
OG0017455
Physical Location & Seq
Reverse (-)
483634 .. 484254
621 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 621 bp
atgccttttcccttcagttttaagcccttcactagacccccggatggattagagaatttacaggtggctgacttaattgacagtgatctgcatgagtggaattttgcggtgctacaggagctattttcgccttcagaagttgaaataatagggcgcattcctctaagtacgagtggtccagaggacagattaacatggtactatgacaagaagggctgttatagtgttcgttctggctatcatgtggctcggaatgcagttgatcgagtatctagggcgtctgcatctaatggttcttttggggtcaatagtaaattctggagaagactctggcatgcacgtacacctcctaaggtacggtcctttatgtggagagtcttaaaaggtattctacctactaaagtttcccttgcacgacgtgtcactctcccacatatgaaatgtgttctatgcaatagttcaattgaggatggtattcatctttttaaggattgtgacattgctggcggtacttggttggctagtaacttgggtgtgcatgtgaagaatattcctggacgtcatttggaggaatgggttcttaatgtcatggaattattgcatggaggtcaatttttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

206

Amino Acids

23.44

Weight (kDa)

9.14

Isoelectric Point (pI)

46.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 74 - 172 3.2e-16 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 562
AciI CCGC 2 cut(s) 107, 507
AcsI RAATTY 3 cut(s) 55, 100, 314
AcuI CTGAAG 1 cut(s) 117
AcyI GRCGYC 2 cut(s) 278, 559
AdeI CACNNNGTG 1 cut(s) 419
AfaI GTAC 5 cut(s) 169, 200, 343, 357, 511
AfiI CCNNNNNNNGG 2 cut(s) 44, 369
AflIII ACRYGT 1 cut(s) 418
AgsI TTSAA 2 cut(s) 143, 462
AjiI CACGTC 1 cut(s) 419
AjnI CCWGG 1 cut(s) 553
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
ApoI RAATTY 3 cut(s) 55, 100, 314
Asp700I GAANNNNTTC 1 cut(s) 576
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 176, 360
AsuC2I CCSGG 1 cut(s) 41
AvaII GGWCC 2 cut(s) 176, 360
AxyI CCTNAGG 1 cut(s) 351
BbsI GAAGAC 1 cut(s) 331
BccI CCATC 2 cut(s) 38, 464
BciT130I CCWGG 1 cut(s) 555
BcnI CCSGG 1 cut(s) 41
BfaI CTAG 3 cut(s) 33, 273, 522
BfmI CTRYAG 1 cut(s) 113
Bme1390I CCNGG 2 cut(s) 41, 555
Bme18I GGWCC 2 cut(s) 176, 360
BmgBI CACGTC 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 176, 360
BmrFI CCNGG 2 cut(s) 41, 555
BmsI GCATC 1 cut(s) 293
BpiI GAAGAC 1 cut(s) 331
BpmI CTGGAG 1 cut(s) 340
BpuMI CCSGG 1 cut(s) 41
BsaAI YACGTR 1 cut(s) 341
BsaHI GRCGYC 2 cut(s) 278, 559
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 2 cut(s) 44, 369
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 498
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 2 cut(s) 49, 475
BseLI CCNNNNNNNGG 2 cut(s) 44, 369
BseMI GCAATG 1 cut(s) 498
BsiSI CCGG 1 cut(s) 41
BslI CCNNNNNNNGG 2 cut(s) 44, 369
BsmI GAATGC 2 cut(s) 156, 259
Bsp143I GATC 2 cut(s) 85, 262
BspACI CCGC 2 cut(s) 107, 507
BsrDI GCAATG 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 2 cut(s) 85, 262
BssNI GRCGYC 2 cut(s) 278, 559
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 2 cut(s) 83, 360
BstACI GRCGYC 2 cut(s) 278, 559
BstBAI YACGTR 1 cut(s) 341
BstC8I GCNNGC 2 cut(s) 336, 505
BstDEI CTNAG 2 cut(s) 164, 351
BstF5I GGATG 2 cut(s) 49, 475
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 2 cut(s) 88, 265
BstMBI GATC 2 cut(s) 85, 262
BstMWI GCNNNNNNNGC 3 cut(s) 118, 127, 254
BstNI CCWGG 1 cut(s) 555
BstNSI RCATGY 2 cut(s) 338, 542
BstSCI CCNGG 2 cut(s) 39, 553
BstSFI CTRYAG 1 cut(s) 113
BstV2I GAAGAC 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 351
BtrI CACGTC 1 cut(s) 419
BtsCI GGATG 2 cut(s) 49, 475
BtsIMutI CAGTG 1 cut(s) 88
Cac8I GCNNGC 2 cut(s) 336, 505
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 176, 360
CseI GACGC 1 cut(s) 267
Csp6I GTAC 5 cut(s) 168, 199, 342, 356, 510
CviAII CATG 7 cut(s) 92, 195, 242, 335, 539, 589, 602
CviJI RGCY 7 cut(s) 25, 68, 121, 216, 237, 248, 521
CviKI_1 RGCY 7 cut(s) 25, 68, 121, 216, 237, 248, 521
CviQI GTAC 5 cut(s) 168, 199, 342, 356, 510
DdeI CTNAG 2 cut(s) 164, 351
DpnI GATC 2 cut(s) 87, 264
DpnII GATC 2 cut(s) 85, 262
DraIII CACNNNGTG 1 cut(s) 419
Eco47I GGWCC 2 cut(s) 176, 360
Eco57I CTGAAG 1 cut(s) 117
Eco81I CCTNAGG 1 cut(s) 351
EcoRII CCWGG 1 cut(s) 553
FaeI CATG 7 cut(s) 95, 198, 245, 338, 542, 592, 605
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 7 cut(s) 91, 194, 241, 334, 538, 588, 601
FauNDI CATATG 1 cut(s) 435
FokI GGATG 2 cut(s) 56, 482
FspBI CTAG 3 cut(s) 33, 273, 522
GlaI GCGC 1 cut(s) 155
GsuI CTGGAG 1 cut(s) 340
HapII CCGG 1 cut(s) 41
HgaI GACGC 1 cut(s) 267
HhaI GCGC 1 cut(s) 156
Hin1I GRCGYC 2 cut(s) 278, 559
Hin1II CATG 7 cut(s) 95, 198, 245, 338, 542, 592, 605
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 2 cut(s) 327, 375
HpaII CCGG 1 cut(s) 41
Hpy166II GTNNAC 1 cut(s) 344
Hpy188I TCNGA 2 cut(s) 136, 252
Hpy188III TCNNGA 2 cut(s) 179, 319
Hpy8I GTNNAC 1 cut(s) 344
Hpy99I CGWCG 1 cut(s) 420
HpyAV CCTTC 4 cut(s) 22, 37, 141, 205
HpyCH4III ACNGT 2 cut(s) 83, 360
HpyCH4IV ACGT 3 cut(s) 340, 418, 559
HpyCH4V TGCA 8 cut(s) 91, 257, 284, 338, 413, 453, 538, 601
HpyF10VI GCNNNNNNNGC 3 cut(s) 118, 127, 254
HpyF3I CTNAG 2 cut(s) 164, 351
HpySE526I ACGT 3 cut(s) 340, 418, 559
Hsp92I GRCGYC 2 cut(s) 278, 559
Hsp92II CATG 7 cut(s) 95, 198, 245, 338, 542, 592, 605
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 2 cut(s) 85, 262
LmnI GCTCC 1 cut(s) 118
LweI GCATC 1 cut(s) 293
MaeI CTAG 3 cut(s) 33, 273, 522
MaeII ACGT 3 cut(s) 340, 418, 559
MaeIII GTNAC 3 cut(s) 421, 494, 524
MalI GATC 2 cut(s) 87, 264
MboI GATC 2 cut(s) 85, 262
MboII GAAGA 2 cut(s) 336, 556
MfeI CAATTG 1 cut(s) 462
MluCI AATT 7 cut(s) 55, 75, 100, 314, 462, 593, 611
MlyI GAGTC 2 cut(s) 321, 384
MnlI CCTC 6 cut(s) 171, 175, 357, 460, 562, 599
MroXI GAANNNNTTC 1 cut(s) 576
MseI TTAA 6 cut(s) 21, 74, 191, 380, 486, 582
MspI CCGG 1 cut(s) 41
MspR9I CCNGG 2 cut(s) 41, 555
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 2 cut(s) 156, 259
MvaI CCWGG 1 cut(s) 555
MwoI GCNNNNNNNGC 3 cut(s) 118, 127, 254
NciI CCSGG 1 cut(s) 41
NdeI CATATG 1 cut(s) 435
NdeII GATC 2 cut(s) 85, 262
NlaIII CATG 7 cut(s) 95, 198, 245, 338, 542, 592, 605
NmuCI GTSAC 2 cut(s) 421, 494
NspI RCATGY 2 cut(s) 338, 542
PaeI GCATGC 1 cut(s) 338
PctI GAATGC 2 cut(s) 156, 259
PdmI GAANNNNTTC 1 cut(s) 576
PfoI TCCNGGA 1 cut(s) 553
PleI GAGTC 2 cut(s) 321, 383
PpsI GAGTC 2 cut(s) 321, 383
Ppu21I YACGTR 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 553
PspGI CCWGG 1 cut(s) 553
PspPI GGNCC 2 cut(s) 176, 360
RsaI GTAC 5 cut(s) 169, 200, 343, 357, 511
RsaNI GTAC 5 cut(s) 168, 199, 342, 356, 510
SaqAI TTAA 6 cut(s) 21, 74, 191, 380, 486, 582
Sau3AI GATC 2 cut(s) 85, 262
Sau96I GGNCC 2 cut(s) 176, 360
SchI GAGTC 2 cut(s) 321, 384
ScrFI CCNGG 2 cut(s) 41, 555
SfaNI GCATC 1 cut(s) 293
SfcI CTRYAG 1 cut(s) 113
SinI GGWCC 2 cut(s) 176, 360
SphI GCATGC 1 cut(s) 338
Sse9I AATT 7 cut(s) 55, 75, 100, 314, 462, 593, 611
SsiI CCGC 2 cut(s) 107, 507
SspI AATATT 1 cut(s) 550
SspMI CTAG 3 cut(s) 33, 273, 522
StyD4I CCNGG 2 cut(s) 39, 553
TaaI ACNGT 2 cut(s) 83, 360
TaiI ACGT 3 cut(s) 343, 421, 562
TaqI TCGA 1 cut(s) 265
TasI AATT 7 cut(s) 55, 75, 100, 314, 462, 593, 611
Tru1I TTAA 6 cut(s) 21, 74, 191, 380, 486, 582
Tru9I TTAA 6 cut(s) 21, 74, 191, 380, 486, 582
TscAI CASTG 1 cut(s) 88
TseFI GTSAC 2 cut(s) 421, 494
Tsp45I GTSAC 2 cut(s) 421, 494
TspDTI ATGAA 2 cut(s) 452, 467
TspRI CASTG 1 cut(s) 88
VpaK11BI GGWCC 2 cut(s) 176, 360
XapI RAATTY 3 cut(s) 55, 100, 314
XceI RCATGY 2 cut(s) 338, 542
XmnI GAANNNNTTC 1 cut(s) 576
XspI CTAG 3 cut(s) 33, 273, 522
ZraI GACGTC 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.