Rh5BG309100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017455
Physical Location & Seq
Reverse (-)
40379450 .. 40379833
384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG309100.1

Sequence Viewer

Length: 384 bp
ATGACTGCGCCGGAATTATCAGTTGCTGGTTTCACTCGTACTAGGGCGGAGAGAGACGCCGATATGTCGGAGCATGGGTCTGTTTCCGCTGGAGCAATGGAGGTGGATCTGGTGGCCTCTCAGGAGGATGAGCCGGCACAACCGTTAGCAAAGAAAAGGAAAACGGTGGCGCTGGGTGACCGGATTTCAGATACGACGGCGAATCATGATTCTGGGAATGTTTTGAGGGATGTGCCTGTGTTCATGGCGCCGAATGTGGAGTCTGCTTTAATTAATTTCCCTATTGGGGGGACTGAGATAGGGAAGATGCGTGAGGCTGGGGTGGAGTTGATTAATGTTCCTTGTCTAGATCCGGATCCCCTTGCGTCCACCATTTGTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

13.42

Weight (kDa)

4.5

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 247
AccIII TCCGGA 1 cut(s) 352
AciI CCGC 2 cut(s) 47, 87
AclWI GGATC 4 cut(s) 114, 344, 350, 363
AcyI GRCGYC 2 cut(s) 57, 248
AfaI GTAC 1 cut(s) 40
AfiI CCNNNNNNNGG 2 cut(s) 286, 287
Alw26I GTCTC 1 cut(s) 48
AlwI GGATC 4 cut(s) 114, 344, 350, 363
AlwNI CAGNNNCTG 1 cut(s) 26
Aor13HI TCCGGA 1 cut(s) 352
AoxI GGCC 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 245, 277
AseI ATTAAT 2 cut(s) 273, 333
AspLEI GCGC 3 cut(s) 10, 172, 250
AsuHPI GGTGA 1 cut(s) 188
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 247
BceAI ACGGC 1 cut(s) 213
BcoDI GTCTC 1 cut(s) 48
BfaI CTAG 2 cut(s) 42, 347
BfoI RGCGCY 2 cut(s) 173, 251
BmiI GGNNCC 2 cut(s) 249, 357
BmsI GCATC 1 cut(s) 297
BpmI CTGGAG 1 cut(s) 111
BsaBI GATNNNNATC 1 cut(s) 354
BsaHI GRCGYC 2 cut(s) 57, 248
BsaWI WCCGGW 2 cut(s) 180, 352
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 287
Bse118I RCCGGY 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 102
Bse8I GATNNNNATC 1 cut(s) 354
BseAI TCCGGA 1 cut(s) 352
BseGI GGATG 2 cut(s) 133, 235
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 2 cut(s) 286, 287
BseMI GCAATG 1 cut(s) 102
BseMII CTCAG 2 cut(s) 134, 285
BseYI CCCAGC 2 cut(s) 172, 317
BshFI GGCC 1 cut(s) 116
BshNI GGYRCC 1 cut(s) 247
BsiSI CCGG 4 cut(s) 11, 134, 181, 353
BslFI GGGAC 1 cut(s) 304
BslI CCNNNNNNNGG 2 cut(s) 286, 287
BsmAI GTCTC 1 cut(s) 48
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 1 cut(s) 304
BsnI GGCC 1 cut(s) 116
Bsp13I TCCGGA 1 cut(s) 352
Bsp143I GATC 3 cut(s) 106, 349, 355
BspACI CCGC 2 cut(s) 47, 87
BspANI GGCC 1 cut(s) 116
BspCNI CTCAG 2 cut(s) 133, 286
BspEI TCCGGA 1 cut(s) 352
BspHI TCATGA 1 cut(s) 205
BspLI GGNNCC 2 cut(s) 249, 357
BspPI GGATC 4 cut(s) 114, 344, 350, 363
BspT107I GGYRCC 1 cut(s) 247
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 133
BssAI RCCGGY 1 cut(s) 133
BssMI GATC 3 cut(s) 106, 349, 355
BssNI GRCGYC 2 cut(s) 57, 248
Bst4CI ACNGT 2 cut(s) 144, 166
BstACI GRCGYC 2 cut(s) 57, 248
BstC8I GCNNGC 1 cut(s) 135
BstDEI CTNAG 2 cut(s) 120, 294
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 2 cut(s) 133, 235
BstH2I RGCGCY 2 cut(s) 173, 251
BstHHI GCGC 3 cut(s) 10, 172, 250
BstKTI GATC 3 cut(s) 109, 352, 358
BstMAI GTCTC 1 cut(s) 48
BstMBI GATC 3 cut(s) 106, 349, 355
BstPI GGTNACC 1 cut(s) 176
BstX2I RGATCY 3 cut(s) 106, 349, 355
BstYI RGATCY 3 cut(s) 106, 349, 355
BsuRI GGCC 1 cut(s) 116
BtsCI GGATG 2 cut(s) 133, 235
Cac8I GCNNGC 1 cut(s) 135
CaiI CAGNNNCTG 1 cut(s) 26
CciI TCATGA 1 cut(s) 205
CfoI GCGC 3 cut(s) 10, 172, 250
Cfr10I RCCGGY 1 cut(s) 133
CseI GACGC 2 cut(s) 65, 354
Csp6I GTAC 1 cut(s) 39
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 3 cut(s) 74, 206, 244
CviJI RGCY 3 cut(s) 116, 133, 317
CviKI_1 RGCY 3 cut(s) 116, 133, 317
CviQI GTAC 1 cut(s) 39
DdeI CTNAG 2 cut(s) 120, 294
DinI GGCGCC 1 cut(s) 249
DpnI GATC 3 cut(s) 108, 351, 357
DpnII GATC 3 cut(s) 106, 349, 355
EciI GGCGGA 1 cut(s) 62
Eco91I GGTNACC 1 cut(s) 176
EcoO65I GGTNACC 1 cut(s) 176
EgeI GGCGCC 1 cut(s) 249
EheI GGCGCC 1 cut(s) 249
Esp3I CGTCTC 1 cut(s) 48
FaeI CATG 3 cut(s) 77, 209, 247
FaiI YATR 4 cut(s) 65, 75, 207, 245
FaqI GGGAC 1 cut(s) 304
FatI CATG 3 cut(s) 73, 205, 243
FokI GGATG 2 cut(s) 140, 242
FspBI CTAG 2 cut(s) 42, 347
GlaI GCGC 3 cut(s) 9, 171, 249
GsaI CCCAGC 2 cut(s) 176, 321
GsuI CTGGAG 1 cut(s) 111
HaeII RGCGCY 2 cut(s) 173, 251
HaeIII GGCC 1 cut(s) 116
HapII CCGG 4 cut(s) 11, 134, 181, 353
HgaI GACGC 2 cut(s) 65, 354
HhaI GCGC 3 cut(s) 10, 172, 250
Hin1I GRCGYC 2 cut(s) 57, 248
Hin1II CATG 3 cut(s) 77, 209, 247
Hin6I GCGC 3 cut(s) 8, 170, 248
HinP1I GCGC 3 cut(s) 8, 170, 248
HinfI GANTC 3 cut(s) 202, 209, 260
HpaII CCGG 4 cut(s) 11, 134, 181, 353
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 1 cut(s) 369
Hpy188I TCNGA 2 cut(s) 70, 190
Hpy188III TCNNGA 4 cut(s) 122, 206, 347, 353
Hpy8I GTNNAC 1 cut(s) 369
Hpy99I CGWCG 1 cut(s) 199
HpyCH4III ACNGT 2 cut(s) 144, 166
HpyF3I CTNAG 2 cut(s) 120, 294
Hsp92I GRCGYC 2 cut(s) 57, 248
Hsp92II CATG 3 cut(s) 77, 209, 247
HspAI GCGC 3 cut(s) 8, 170, 248
KasI GGCGCC 1 cut(s) 247
Kpn2I TCCGGA 1 cut(s) 352
KroI GCCGGC 1 cut(s) 133
KroNI GCCGGC 1 cut(s) 135
Kzo9I GATC 3 cut(s) 106, 349, 355
LmnI GCTCC 2 cut(s) 70, 92
LweI GCATC 1 cut(s) 297
MaeI CTAG 2 cut(s) 42, 347
MaeIII GTNAC 1 cut(s) 176
MalI GATC 3 cut(s) 108, 351, 357
MboI GATC 3 cut(s) 106, 349, 355
MboII GAAGA 1 cut(s) 316
MflI RGATCY 3 cut(s) 106, 349, 355
MluCI AATT 3 cut(s) 14, 270, 274
Mly113I GGCGCC 1 cut(s) 248
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 1 cut(s) 48
MnlI CCTC 5 cut(s) 94, 118, 127, 219, 307
MroI TCCGGA 1 cut(s) 352
MroNI GCCGGC 1 cut(s) 133
MseI TTAA 3 cut(s) 269, 273, 333
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 4 cut(s) 11, 134, 181, 353
NaeI GCCGGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 248
NdeII GATC 3 cut(s) 106, 349, 355
NgoMIV GCCGGC 1 cut(s) 133
NlaIII CATG 3 cut(s) 77, 209, 247
NlaIV GGNNCC 2 cut(s) 249, 357
NmuCI GTSAC 1 cut(s) 176
PacI TTAATTAA 1 cut(s) 273
PagI TCATGA 1 cut(s) 205
PdiI GCCGGC 1 cut(s) 135
PfeI GAWTC 2 cut(s) 202, 209
PleI GAGTC 1 cut(s) 268
PluTI GGCGCC 1 cut(s) 251
PpsI GAGTC 1 cut(s) 268
PshBI ATTAAT 2 cut(s) 273, 333
PspEI GGTNACC 1 cut(s) 176
PspFI CCCAGC 2 cut(s) 172, 317
PspN4I GGNNCC 2 cut(s) 249, 357
PstNI CAGNNNCTG 1 cut(s) 26
PsuI RGATCY 3 cut(s) 106, 349, 355
RsaI GTAC 1 cut(s) 40
RsaNI GTAC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 269, 273, 333
Sau3AI GATC 3 cut(s) 106, 349, 355
SchI GAGTC 1 cut(s) 269
SetI ASST 1 cut(s) 105
SfaNI GCATC 1 cut(s) 297
SfoI GGCGCC 1 cut(s) 249
Sse9I AATT 3 cut(s) 14, 270, 274
SsiI CCGC 2 cut(s) 47, 87
SspDI GGCGCC 1 cut(s) 247
SspMI CTAG 2 cut(s) 42, 347
TaaI ACNGT 2 cut(s) 144, 166
TasI AATT 3 cut(s) 14, 270, 274
TfiI GAWTC 2 cut(s) 202, 209
Tru1I TTAA 3 cut(s) 269, 273, 333
Tru9I TTAA 3 cut(s) 269, 273, 333
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 232
VspI ATTAAT 2 cut(s) 273, 333
XbaI TCTAGA 1 cut(s) 346
XspI CTAG 2 cut(s) 42, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.