Rh2BG180700

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017455
Physical Location & Seq
Reverse (-)
16073538 .. 16077326
3789 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG180700.1

Sequence Viewer

Length: 789 bp
ATGGAGGTGGATCTGGTGGCCTCTCAGGAGGATGAGCCGGCACAACCGTTAGCAAAGAAAAGGAAAACGGTGGCGCTGGGTGACCGGATTTCAGATGCGACGACGAATCATGATTCTGGGAATGTTTTGAGGGATGTGCCTATGTTCATGGCGCCGAATGTGGAGTCTGCTTTAATTAATTTCCCTATTGGGGGGACTGAGATAGGGAAGACGCGTGAGGTTGGGGTGGAGTTGATTAATGTCCCTTGTCTAGATCTGGATCCCGTGCCTCCACCATCTGTGATTGAGCCTGTCATTGCGTCTTTTTTGAATTTTCCTAGGCGGGACTATGGGGTTTTGAATGTTAATGGGTTGAATGGGTCTTCATCTGCGGCGAGGCCCAAGAGAACAAAAAAGAGTGTTTCTCATTTGGATGATAAGAAAGTCAGCGAGATTGGGCTAGAAGTGGTTAATGATTGTAGGCAGAAGTGTGGGAGCCAGCCCCATGCATCCCATGCATCTTCTGTACGTACTAACAAGGTGGCTAGGAAAGGTTTTCGACCGTTCACTAGACCCCCTGAAGGCTTGGGTGACTTATGTGTTACTGACCTTTTGGACTTGGATATGCACGAGTGGGATATGGATTTGCTTAAGGCTCTCTTTTCCCCATCAGAGGTTGAGGTCATTGGGCGGATCCCTCTTAGTGTGCGTGAGTCGGAGGATAGACTTATTTGGCACTATGATAGGAAGGGCTGCTACACTGCTCGTAATGGGTACCATGTAGCGCGTACAGCTGCTGAACGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

262

Amino Acids

28.9

Weight (kDa)

6.02

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 753
AccB1I GGYRCC 2 cut(s) 151, 753
AccII CGCG 2 cut(s) 214, 766
AciI CCGC 3 cut(s) 322, 371, 670
AclWI GGATC 5 cut(s) 18, 254, 267, 667, 680
AcsI RAATTY 1 cut(s) 310
AcuI CTGAAG 1 cut(s) 579
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 4 cut(s) 507, 511, 755, 769
AfiI CCNNNNNNNGG 4 cut(s) 190, 191, 560, 652
AflII CTTAAG 1 cut(s) 629
AflIII ACRYGT 1 cut(s) 212
AgsI TTSAA 3 cut(s) 310, 340, 355
AluBI AGCT 1 cut(s) 773
AluI AGCT 1 cut(s) 773
AlwI GGATC 5 cut(s) 18, 254, 267, 667, 680
AlwNI CAGNNNCTG 1 cut(s) 776
AoxI GGCC 2 cut(s) 18, 377
ApeKI GCWGC 2 cut(s) 732, 773
ApoI RAATTY 1 cut(s) 310
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AseI ATTAAT 2 cut(s) 177, 237
Asp718I GGTACC 1 cut(s) 753
AspA2I CCTAGG 1 cut(s) 317
AspLEI GCGC 3 cut(s) 76, 154, 766
AspS9I GGNCC 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 92, 581
AvrII CCTAGG 1 cut(s) 317
BamHI GGATCC 2 cut(s) 259, 672
BanI GGYRCC 2 cut(s) 151, 753
BarI GAAGNNNNNNTAC 2 cut(s) 719, 751
BauI CACGAG 1 cut(s) 608
BbsI GAAGAC 2 cut(s) 215, 354
BbvI GCAGC 2 cut(s) 719, 760
BccI CCATC 2 cut(s) 283, 655
BfaI CTAG 5 cut(s) 251, 318, 440, 525, 549
BfoI RGCGCY 2 cut(s) 77, 155
BfrI CTTAAG 1 cut(s) 629
BglII AGATCT 1 cut(s) 253
BisI GCNGC 3 cut(s) 372, 733, 774
BlnI CCTAGG 1 cut(s) 317
BlsI GCNGC 3 cut(s) 373, 734, 775
BmgT120I GGNCC 1 cut(s) 378
BmiI GGNNCC 5 cut(s) 153, 261, 476, 674, 755
BmsI GCATC 3 cut(s) 85, 497, 506
BpiI GAAGAC 2 cut(s) 215, 354
BplI GAGNNNNNCTC 2 cut(s) 388, 420
BsaAI YACGTR 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 258
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 1 cut(s) 84
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 191, 560, 652
Bse118I RCCGGY 1 cut(s) 37
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 258
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 4 cut(s) 37, 139, 418, 488
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 4 cut(s) 190, 191, 560, 652
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 2 cut(s) 38, 189
BseXI GCAGC 2 cut(s) 719, 760
BseYI CCCAGC 1 cut(s) 76
Bsh1236I CGCG 2 cut(s) 214, 766
Bsh1285I CGRYCG 1 cut(s) 542
BshFI GGCC 2 cut(s) 20, 379
BshNI GGYRCC 2 cut(s) 151, 753
BsiEI CGRYCG 1 cut(s) 542
BsiSI CCGG 2 cut(s) 38, 85
BslFI GGGAC 3 cut(s) 208, 227, 338
BslI CCNNNNNNNGG 4 cut(s) 190, 191, 560, 652
BsmFI GGGAC 3 cut(s) 208, 227, 338
BsnI GGCC 2 cut(s) 20, 379
Bsp143I GATC 4 cut(s) 10, 253, 259, 672
BspACI CCGC 3 cut(s) 322, 371, 670
BspANI GGCC 2 cut(s) 20, 379
BspCNI CTCAG 2 cut(s) 37, 190
BspFNI CGCG 2 cut(s) 214, 766
BspHI TCATGA 1 cut(s) 109
BspLI GGNNCC 5 cut(s) 153, 261, 476, 674, 755
BspPI GGATC 5 cut(s) 18, 254, 267, 667, 680
BspT107I GGYRCC 2 cut(s) 151, 753
BspTI CTTAAG 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 37
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 4 cut(s) 10, 253, 259, 672
BssNI GRCGYC 1 cut(s) 152
BssSI CACGAG 1 cut(s) 608
BssT1I CCWWGG 1 cut(s) 317
Bst2BI CACGAG 1 cut(s) 608
Bst4CI ACNGT 3 cut(s) 48, 70, 543
BstACI GRCGYC 1 cut(s) 152
BstAFI CTTAAG 1 cut(s) 629
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 39, 479
BstDEI CTNAG 3 cut(s) 24, 198, 680
BstEII GGTNACC 1 cut(s) 80
BstF5I GGATG 4 cut(s) 37, 139, 418, 488
BstFNI CGCG 2 cut(s) 214, 766
BstH2I RGCGCY 2 cut(s) 77, 155
BstHHI GCGC 3 cut(s) 76, 154, 766
BstKTI GATC 4 cut(s) 13, 256, 262, 675
BstMBI GATC 4 cut(s) 10, 253, 259, 672
BstMCI CGRYCG 1 cut(s) 542
BstMWI GCNNNNNNNGC 2 cut(s) 494, 770
BstPI GGTNACC 1 cut(s) 80
BstSNI TACGTA 1 cut(s) 509
BstUI CGCG 2 cut(s) 214, 766
BstV1I GCAGC 2 cut(s) 719, 760
BstV2I GAAGAC 2 cut(s) 215, 354
BstX2I RGATCY 4 cut(s) 10, 253, 259, 672
BstYI RGATCY 4 cut(s) 10, 253, 259, 672
BsuRI GGCC 2 cut(s) 20, 379
BtsCI GGATG 4 cut(s) 37, 139, 418, 488
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 1 cut(s) 738
Cac8I GCNNGC 2 cut(s) 39, 479
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 109
CfoI GCGC 3 cut(s) 76, 154, 766
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 1 cut(s) 378
CseI GACGC 2 cut(s) 220, 288
Csp6I GTAC 4 cut(s) 506, 510, 754, 768
CviAII CATG 5 cut(s) 110, 148, 485, 494, 758
CviQI GTAC 4 cut(s) 506, 510, 754, 768
DdeI CTNAG 3 cut(s) 24, 198, 680
DinI GGCGCC 1 cut(s) 153
DpnI GATC 4 cut(s) 12, 255, 261, 674
DpnII GATC 4 cut(s) 10, 253, 259, 672
EciI GGCGGA 1 cut(s) 685
Eco105I TACGTA 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 579
Eco91I GGTNACC 1 cut(s) 80
EcoO65I GGTNACC 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 2 cut(s) 490, 499
EgeI GGCGCC 1 cut(s) 153
EheI GGCGCC 1 cut(s) 153
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 5 cut(s) 113, 151, 488, 497, 761
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 3 cut(s) 208, 227, 338
FatI CATG 5 cut(s) 109, 147, 484, 493, 757
FauI CCCGC 1 cut(s) 315
Fnu4HI GCNGC 3 cut(s) 372, 733, 774
FokI GGATG 4 cut(s) 44, 146, 425, 475
Fsp4HI GCNGC 3 cut(s) 372, 733, 774
FspBI CTAG 5 cut(s) 251, 318, 440, 525, 549
GlaI GCGC 3 cut(s) 75, 153, 765
GluI GCNGC 3 cut(s) 372, 733, 774
GsaI CCCAGC 1 cut(s) 80
HaeII RGCGCY 2 cut(s) 77, 155
HaeIII GGCC 2 cut(s) 20, 379
HapII CCGG 2 cut(s) 38, 85
HgaI GACGC 2 cut(s) 220, 288
HhaI GCGC 3 cut(s) 76, 154, 766
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 5 cut(s) 113, 151, 488, 497, 761
Hin6I GCGC 3 cut(s) 74, 152, 764
HinP1I GCGC 3 cut(s) 74, 152, 764
HinfI GANTC 4 cut(s) 106, 113, 164, 692
HpaII CCGG 2 cut(s) 38, 85
HphI GGTGA 2 cut(s) 92, 581
Hpy166II GTNNAC 1 cut(s) 546
Hpy188I TCNGA 3 cut(s) 94, 652, 697
Hpy188III TCNNGA 4 cut(s) 26, 110, 251, 257
Hpy8I GTNNAC 1 cut(s) 546
Hpy99I CGWCG 2 cut(s) 103, 106
HpyAV CCTTC 2 cut(s) 554, 721
HpyCH4III ACNGT 3 cut(s) 48, 70, 543
HpyCH4IV ACGT 2 cut(s) 508, 781
HpyCH4V TGCA 3 cut(s) 488, 497, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 770
HpyF3I CTNAG 3 cut(s) 24, 198, 680
HpySE526I ACGT 2 cut(s) 508, 781
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 5 cut(s) 113, 151, 488, 497, 761
HspAI GCGC 3 cut(s) 74, 152, 764
KasI GGCGCC 1 cut(s) 151
KpnI GGTACC 1 cut(s) 757
KroI GCCGGC 1 cut(s) 37
KroNI GCCGGC 1 cut(s) 39
Kzo9I GATC 4 cut(s) 10, 253, 259, 672
LmnI GCTCC 1 cut(s) 474
LpnPI CCDG 9 cut(s) 11, 51, 62, 98, 102, 242, 303, 491, 570
Lsp1109I GCAGC 2 cut(s) 719, 760
LweI GCATC 3 cut(s) 85, 497, 506
MaeI CTAG 5 cut(s) 251, 318, 440, 525, 549
MaeII ACGT 2 cut(s) 508, 781
MaeIII GTNAC 3 cut(s) 80, 569, 580
MalI GATC 4 cut(s) 12, 255, 261, 674
MboI GATC 4 cut(s) 10, 253, 259, 672
MboII GAAGA 3 cut(s) 220, 354, 492
MflI RGATCY 4 cut(s) 10, 253, 259, 672
MluCI AATT 3 cut(s) 174, 178, 310
MluI ACGCGT 1 cut(s) 212
Mly113I GGCGCC 1 cut(s) 152
MlyI GAGTC 2 cut(s) 173, 701
MmeI TCCRAC 1 cut(s) 675
Mph1103I ATGCAT 2 cut(s) 490, 499
MroNI GCCGGC 1 cut(s) 37
MseI TTAA 6 cut(s) 173, 177, 237, 345, 450, 630
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 773
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 2 cut(s) 38, 85
MvnI CGCG 2 cut(s) 214, 766
MwoI GCNNNNNNNGC 2 cut(s) 494, 770
NaeI GCCGGC 1 cut(s) 39
NarI GGCGCC 1 cut(s) 152
NdeII GATC 4 cut(s) 10, 253, 259, 672
NgoMIV GCCGGC 1 cut(s) 37
NlaIII CATG 5 cut(s) 113, 151, 488, 497, 761
NlaIV GGNNCC 5 cut(s) 153, 261, 476, 674, 755
NmuCI GTSAC 2 cut(s) 80, 569
NsiI ATGCAT 2 cut(s) 490, 499
PacI TTAATTAA 1 cut(s) 177
PagI TCATGA 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 39
PfeI GAWTC 2 cut(s) 106, 113
PkrI GCNGC 3 cut(s) 373, 734, 775
PleI GAGTC 2 cut(s) 172, 700
PluTI GGCGCC 1 cut(s) 155
PpsI GAGTC 2 cut(s) 172, 700
Ppu21I YACGTR 1 cut(s) 509
PshBI ATTAAT 2 cut(s) 177, 237
PspEI GGTNACC 1 cut(s) 80
PspFI CCCAGC 1 cut(s) 76
PspN4I GGNNCC 5 cut(s) 153, 261, 476, 674, 755
PspPI GGNCC 1 cut(s) 378
PstNI CAGNNNCTG 1 cut(s) 776
PsuI RGATCY 4 cut(s) 10, 253, 259, 672
PvuII CAGCTG 1 cut(s) 773
RsaI GTAC 4 cut(s) 507, 511, 755, 769
RsaNI GTAC 4 cut(s) 506, 510, 754, 768
RseI CAYNNNNRTG 1 cut(s) 411
SaqAI TTAA 6 cut(s) 173, 177, 237, 345, 450, 630
SatI GCNGC 3 cut(s) 372, 733, 774
Sau3AI GATC 4 cut(s) 10, 253, 259, 672
Sau96I GGNCC 1 cut(s) 378
SchI GAGTC 2 cut(s) 173, 701
SfaNI GCATC 3 cut(s) 85, 497, 506
SfoI GGCGCC 1 cut(s) 153
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SnaBI TACGTA 1 cut(s) 509
Sse9I AATT 3 cut(s) 174, 178, 310
SsiI CCGC 3 cut(s) 322, 371, 670
SspDI GGCGCC 1 cut(s) 151
SspMI CTAG 5 cut(s) 251, 318, 440, 525, 549
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 3 cut(s) 48, 70, 543
TaiI ACGT 2 cut(s) 511, 784
TaqI TCGA 1 cut(s) 538
TasI AATT 3 cut(s) 174, 178, 310
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 2 cut(s) 106, 113
Tru1I TTAA 6 cut(s) 173, 177, 237, 345, 450, 630
Tru9I TTAA 6 cut(s) 173, 177, 237, 345, 450, 630
TscAI CASTG 1 cut(s) 745
TseFI GTSAC 2 cut(s) 80, 569
TseI GCWGC 2 cut(s) 732, 773
Tsp45I GTSAC 2 cut(s) 80, 569
TspDTI ATGAA 2 cut(s) 136, 354
TspRI CASTG 1 cut(s) 745
Vha464I CTTAAG 1 cut(s) 629
VspI ATTAAT 2 cut(s) 177, 237
XapI RAATTY 1 cut(s) 310
XbaI TCTAGA 1 cut(s) 250
XmaJI CCTAGG 1 cut(s) 317
XspI CTAG 5 cut(s) 251, 318, 440, 525, 549
Zsp2I ATGCAT 2 cut(s) 490, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.