Rmu_sc0000596.1_g000019

UPF0496 protein At4g34320-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000596.1
OG0017240
Physical Location & Seq
Forward (+)
100994 .. 106045
5052 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1068 bp
atgacggatctgagtaagttcgtagagtcagctcgtgaaaatcataaatttattcaatctgttgttcaacagtactttgacaacgggctgccaactggagtcggaggaagtcgctgcaggatgattttggagaaactgaagaagctcaagacctctgatgaccactgtgctgaaaggctgctcgaacagatcgaatctttgcgcaatgagtacgaaaatacgctcgaaaattctgaacttgcaaaacacgagattgagatggagcatgaacgtgcatgtgctctggaaaaacgaaaaacaaagaaaaataaacgcactgcgacaactttgctcatttgcggcactgtggcagctactatggttggtcatccattcatgggtgccgctgccgctatggttgttcgtcgacaagtttctaaagcctgtagctcaagaactaaagaggaagaacaacgacatgaaattgctttcaaggatcactacgcaatggaaggaaaaatgattattagcattaaggagctgggcagcattaagtttctcattgataaggttgtaaatgagatcgattcactcttgagaagtgtagagggagcaattaacactggagaagtagatttcgcaatgaagactattaagaataagatggaaacagtcgaagtttttcgactgacccgacccgaacccggcgttccgacccgacttttccggccgactgcggtggtctccggccgtgaaactttccagacaggatcgcctccgccgtctggtagtccctctggtgctctctatcgccgattcttctccacggcggcgctgcaaggtggtgcagccttgtgttttcggacccggccggaaaacacaactccggcgacctcgtggcttcaggcttcttgggttgagttcagaacaagctcactggtcgatccttggtgtttgtttcgaccggtttacgtgaaaactcgatcaactcggattggatcactgttcacgaattgtgaggtagttttcgatcccttaagcttgttttctgacttcgatccagttatgaaagttcacaagcatgcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF677

Protein Analysis

355

Amino Acids

39.95

Weight (kDa)

9.03

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF677 PF05055 3 - 217 5.5e-15 Protein of unknown function (DUF677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 380
AccI GTMKAC 1 cut(s) 406
AciI CCGC 6 cut(s) 339, 384, 390, 716, 758, 809
AclWI GGATC 7 cut(s) 15, 483, 757, 917, 985, 1004, 1031
AcoI YGGCCR 3 cut(s) 707, 727, 848
AcsI RAATTY 2 cut(s) 47, 229
AcuI CTGAAG 2 cut(s) 158, 866
AfaI GTAC 2 cut(s) 74, 212
AfiI CCNNNNNNNGG 3 cut(s) 377, 683, 764
AflII CTTAAG 1 cut(s) 1015
AgeI ACCGGT 1 cut(s) 943
AgsI TTSAA 3 cut(s) 56, 68, 472
AluBI AGCT 7 cut(s) 32, 145, 353, 429, 520, 912, 1020
AluI AGCT 7 cut(s) 32, 145, 353, 429, 520, 912, 1020
Alw21I GWGCWC 2 cut(s) 283, 784
Alw26I GTCTC 1 cut(s) 727
AlwI GGATC 7 cut(s) 15, 483, 757, 917, 985, 1004, 1031
AoxI GGCC 3 cut(s) 707, 727, 848
ApeKI GCWGC 8 cut(s) 88, 114, 178, 350, 386, 525, 814, 827
ApoI RAATTY 2 cut(s) 47, 229
AsiGI ACCGGT 1 cut(s) 943
Asp700I GAANNNNTTC 1 cut(s) 660
AspLEI GCGC 2 cut(s) 204, 814
AspS9I GGNCC 1 cut(s) 843
AsuC2I CCSGG 2 cut(s) 684, 847
AvaII GGWCC 1 cut(s) 843
BanI GGYRCC 1 cut(s) 380
BauI CACGAG 3 cut(s) 33, 248, 874
BbsI GAAGAC 1 cut(s) 632
Bbv12I GWGCWC 2 cut(s) 283, 784
BbvI GCAGC 8 cut(s) 75, 101, 165, 362, 373, 537, 801, 839
BccI CCATC 2 cut(s) 253, 637
BceAI ACGGC 3 cut(s) 714, 745, 822
BcgI CGANNNNNNTGC 2 cut(s) 310, 344
BcnI CCSGG 2 cut(s) 684, 847
BcoDI GTCTC 1 cut(s) 727
BfmI CTRYAG 2 cut(s) 115, 424
BfoI RGCGCY 1 cut(s) 815
BfrI CTTAAG 1 cut(s) 1015
BmcAI AGTACT 1 cut(s) 74
Bme1390I CCNGG 2 cut(s) 684, 847
Bme18I GGWCC 1 cut(s) 843
BmgT120I GGNCC 1 cut(s) 843
BmiI GGNNCC 2 cut(s) 382, 845
BmrFI CCNGG 2 cut(s) 684, 847
BpiI GAAGAC 1 cut(s) 632
BpmI CTGGAG 2 cut(s) 117, 624
BpuEI CTTGAG 3 cut(s) 131, 415, 595
BpuMI CCSGG 2 cut(s) 684, 847
Bsa29I ATCGAT 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 952
BsaI GGTCTC 1 cut(s) 727
BsaJI CCNNGG 2 cut(s) 804, 926
BsaWI WCCGGW 1 cut(s) 943
Bsc4I CCNNNNNNNGG 3 cut(s) 377, 683, 764
Bse118I RCCGGY 1 cut(s) 943
Bse1I ACTGG 4 cut(s) 100, 607, 921, 1040
Bse3DI GCAATG 3 cut(s) 211, 492, 627
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 2 cut(s) 804, 926
BseGI GGATG 2 cut(s) 126, 367
BseLI CCNNNNNNNGG 3 cut(s) 377, 683, 764
BseMI GCAATG 3 cut(s) 211, 492, 627
BseNI ACTGG 4 cut(s) 100, 607, 921, 1040
BseX3I CGGCCG 3 cut(s) 707, 727, 848
BseXI GCAGC 8 cut(s) 75, 101, 165, 362, 373, 537, 801, 839
BseYI CCCAGC 1 cut(s) 520
BsgI GTGCAG 1 cut(s) 846
Bsh1285I CGRYCG 4 cut(s) 710, 730, 851, 944
BshFI GGCC 3 cut(s) 709, 729, 850
BshNI GGYRCC 1 cut(s) 380
BshTI ACCGGT 1 cut(s) 943
BshVI ATCGAT 1 cut(s) 564
BsiEI CGRYCG 4 cut(s) 710, 730, 851, 944
BsiHKAI GWGCWC 2 cut(s) 283, 784
BsiSI CCGG 7 cut(s) 684, 706, 726, 847, 851, 866, 944
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 3 cut(s) 377, 683, 764
BsmAI GTCTC 1 cut(s) 727
BsmFI GGGAC 1 cut(s) 756
BsnI GGCC 3 cut(s) 709, 729, 850
Bso31I GGTCTC 1 cut(s) 727
Bsp1286I GDGCHC 2 cut(s) 283, 784
BspACI CCGC 6 cut(s) 339, 384, 390, 716, 758, 809
BspANI GGCC 3 cut(s) 709, 729, 850
BspDI ATCGAT 1 cut(s) 564
BspLI GGNNCC 2 cut(s) 382, 845
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 7 cut(s) 15, 483, 757, 917, 985, 1004, 1031
BspT107I GGYRCC 1 cut(s) 380
BspTI CTTAAG 1 cut(s) 1015
BspTNI GGTCTC 1 cut(s) 727
BsrDI GCAATG 3 cut(s) 211, 492, 627
BsrFI RCCGGY 1 cut(s) 943
BsrI ACTGG 4 cut(s) 100, 607, 921, 1040
BssAI RCCGGY 1 cut(s) 943
BssECI CCNNGG 2 cut(s) 804, 926
BssSI CACGAG 3 cut(s) 33, 248, 874
BssT1I CCWWGG 1 cut(s) 926
Bst2BI CACGAG 3 cut(s) 33, 248, 874
Bst4CI ACNGT 5 cut(s) 72, 167, 346, 652, 984
BstAFI CTTAAG 1 cut(s) 1015
BstBAI YACGTR 1 cut(s) 952
BstC8I GCNNGC 1 cut(s) 1062
BstDEI CTNAG 2 cut(s) 11, 1065
BstDSI CCRYGG 1 cut(s) 804
BstF5I GGATG 2 cut(s) 126, 367
BstH2I RGCGCY 1 cut(s) 815
BstHHI GCGC 2 cut(s) 204, 814
BstMAI GTCTC 1 cut(s) 727
BstMCI CGRYCG 4 cut(s) 710, 730, 851, 944
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNSI RCATGY 2 cut(s) 279, 1064
BstSCI CCNGG 2 cut(s) 682, 845
BstSFI CTRYAG 2 cut(s) 115, 424
BstV1I GCAGC 8 cut(s) 75, 101, 165, 362, 373, 537, 801, 839
BstV2I GAAGAC 1 cut(s) 632
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BstZI CGGCCG 3 cut(s) 707, 727, 848
Bsu15I ATCGAT 1 cut(s) 564
BsuRI GGCC 3 cut(s) 709, 729, 850
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 804
BtsCI GGATG 2 cut(s) 126, 367
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 6 cut(s) 163, 315, 342, 600, 914, 980
Cac8I GCNNGC 1 cut(s) 1062
CfoI GCGC 2 cut(s) 204, 814
Cfr10I RCCGGY 1 cut(s) 943
Cfr13I GGNCC 1 cut(s) 843
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 2 cut(s) 73, 211
CspAI ACCGGT 1 cut(s) 943
CviAII CATG 5 cut(s) 266, 276, 376, 458, 1061
CviQI GTAC 2 cut(s) 73, 211
DdeI CTNAG 2 cut(s) 11, 1065
EaeI YGGCCR 3 cut(s) 707, 727, 848
EagI CGGCCG 3 cut(s) 707, 727, 848
EciI GGCGGA 1 cut(s) 747
EclXI CGGCCG 3 cut(s) 707, 727, 848
Eco130I CCWWGG 1 cut(s) 926
Eco31I GGTCTC 1 cut(s) 727
Eco47I GGWCC 1 cut(s) 843
Eco52I CGGCCG 3 cut(s) 707, 727, 848
Eco57I CTGAAG 2 cut(s) 158, 866
EcoT14I CCWWGG 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 926
FaeI CATG 5 cut(s) 269, 279, 379, 461, 1064
FaiI YATR 9 cut(s) 45, 267, 277, 359, 377, 395, 459, 1046, 1062
FaqI GGGAC 1 cut(s) 756
FatI CATG 5 cut(s) 265, 275, 375, 457, 1060
FblI GTMKAC 1 cut(s) 406
FokI GGATG 2 cut(s) 133, 354
FspI TGCGCA 1 cut(s) 203
GlaI GCGC 2 cut(s) 203, 813
GsaI CCCAGC 1 cut(s) 524
GsuI CTGGAG 2 cut(s) 117, 624
HaeII RGCGCY 1 cut(s) 815
HaeIII GGCC 3 cut(s) 709, 729, 850
HapII CCGG 7 cut(s) 684, 706, 726, 847, 851, 866, 944
HhaI GCGC 2 cut(s) 204, 814
Hin1II CATG 5 cut(s) 269, 279, 379, 461, 1064
Hin6I GCGC 2 cut(s) 202, 812
HinP1I GCGC 2 cut(s) 202, 812
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HindIII AAGCTT 1 cut(s) 1018
HinfI GANTC 5 cut(s) 26, 99, 194, 566, 795
HpaII CCGG 7 cut(s) 684, 706, 726, 847, 851, 866, 944
Hpy166II GTNNAC 4 cut(s) 407, 949, 987, 1054
Hpy188I TCNGA 9 cut(s) 12, 104, 157, 235, 693, 843, 905, 972, 1030
Hpy188III TCNNGA 7 cut(s) 35, 148, 284, 432, 574, 742, 988
Hpy8I GTNNAC 4 cut(s) 407, 949, 987, 1054
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 1 cut(s) 485
HpyCH4III ACNGT 5 cut(s) 72, 167, 346, 652, 984
HpyCH4IV ACGT 2 cut(s) 271, 951
HpyCH4V TGCA 5 cut(s) 117, 242, 275, 817, 827
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 2 cut(s) 11, 1065
HpySE526I ACGT 2 cut(s) 271, 951
Hsp92II CATG 5 cut(s) 269, 279, 379, 461, 1064
HspAI GCGC 2 cut(s) 202, 812
LmnI GCTCC 3 cut(s) 262, 517, 590
Lsp1109I GCAGC 8 cut(s) 75, 101, 165, 362, 373, 537, 801, 839
MaeII ACGT 2 cut(s) 271, 951
MboII GAAGA 4 cut(s) 151, 458, 637, 790
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 2 cut(s) 283, 784
MluCI AATT 5 cut(s) 47, 229, 462, 594, 991
MlyI GAGTC 2 cut(s) 35, 108
MmeI TCCRAC 2 cut(s) 82, 716
MnlI CCTC 8 cut(s) 98, 163, 436, 580, 765, 784, 883, 991
MroXI GAANNNNTTC 1 cut(s) 660
MseI TTAA 5 cut(s) 513, 531, 597, 633, 1016
MslI CAYNNNNRTG 2 cut(s) 270, 1059
MspA1I CMGCKG 1 cut(s) 386
MspCI CTTAAG 1 cut(s) 1015
MspI CCGG 7 cut(s) 684, 706, 726, 847, 851, 866, 944
MspR9I CCNGG 2 cut(s) 684, 847
MwoI GCNNNNNNNGC 1 cut(s) 389
NciI CCSGG 2 cut(s) 684, 847
NlaIII CATG 5 cut(s) 269, 279, 379, 461, 1064
NlaIV GGNNCC 2 cut(s) 382, 845
NsbI TGCGCA 1 cut(s) 203
NspI RCATGY 2 cut(s) 279, 1064
PaeI GCATGC 1 cut(s) 1064
PcsI WCGNNNNNNNCGW 3 cut(s) 189, 670, 758
PdmI GAANNNNTTC 1 cut(s) 660
PfeI GAWTC 3 cut(s) 194, 566, 795
PinAI ACCGGT 1 cut(s) 943
PleI GAGTC 2 cut(s) 34, 107
PpsI GAGTC 2 cut(s) 34, 107
Ppu21I YACGTR 1 cut(s) 952
PspFI CCCAGC 1 cut(s) 520
PspN4I GGNNCC 2 cut(s) 382, 845
PspPI GGNCC 1 cut(s) 843
PstI CTGCAG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 2 cut(s) 74, 212
RsaNI GTAC 2 cut(s) 73, 211
RseI CAYNNNNRTG 2 cut(s) 270, 1059
SalI GTCGAC 1 cut(s) 405
SaqAI TTAA 5 cut(s) 513, 531, 597, 633, 1016
Sau96I GGNCC 1 cut(s) 843
ScaI AGTACT 1 cut(s) 74
SchI GAGTC 2 cut(s) 35, 108
ScrFI CCNGG 2 cut(s) 684, 847
SduI GDGCHC 2 cut(s) 283, 784
SfcI CTRYAG 2 cut(s) 115, 424
SinI GGWCC 1 cut(s) 843
SmiMI CAYNNNNRTG 2 cut(s) 270, 1059
SmlI CTYRAG 4 cut(s) 146, 430, 574, 1015
SmoI CTYRAG 4 cut(s) 146, 430, 574, 1015
SphI GCATGC 1 cut(s) 1064
Sse9I AATT 5 cut(s) 47, 229, 462, 594, 991
SsiI CCGC 6 cut(s) 339, 384, 390, 716, 758, 809
StyD4I CCNGG 2 cut(s) 682, 845
StyI CCWWGG 1 cut(s) 926
TaaI ACNGT 5 cut(s) 72, 167, 346, 652, 984
TaiI ACGT 2 cut(s) 274, 954
TasI AATT 5 cut(s) 47, 229, 462, 594, 991
TatI WGTACW 1 cut(s) 72
TauI GCSGC 4 cut(s) 342, 386, 392, 812
TfiI GAWTC 3 cut(s) 194, 566, 795
Tru1I TTAA 5 cut(s) 513, 531, 597, 633, 1016
Tru9I TTAA 5 cut(s) 513, 531, 597, 633, 1016
TscAI CASTG 6 cut(s) 170, 322, 349, 607, 921, 987
TseI GCWGC 8 cut(s) 88, 114, 178, 350, 386, 525, 814, 827
TspDTI ATGAA 5 cut(s) 282, 364, 474, 638, 1061
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 6 cut(s) 170, 322, 349, 607, 921, 987
Vha464I CTTAAG 1 cut(s) 1015
VpaK11BI GGWCC 1 cut(s) 843
XapI RAATTY 2 cut(s) 47, 229
XceI RCATGY 2 cut(s) 279, 1064
XmiI GTMKAC 1 cut(s) 406
XmnI GAANNNNTTC 1 cut(s) 660
ZrmI AGTACT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.