Rmu_sc0000723.1_g000006

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000723.1
OG0005633
Physical Location & Seq
Reverse (-)
32103 .. 33733
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 663 bp
ctggattatgacaacaaaagaatcacaactttcaataacctaatcaacctcctgcaagtgactgagagacataataagattcttttgaataacaatgtcaggcccgttgggacaaagaaaattctcgaggctaattatggcaaagtgaaaggtgggaagaaccccaatgcaatgggggttggacgtgctgatccctacccacatggcaacaatgcaccacgtggaaaggaacatgtgggttgtggtatgggctgtggaggccctcccaatgtatggcgcagaaatggtggtactagccctagtggtcatggaaacaaggtgcaaaaggcacctaagaacccttcggtcaaaaaggaaagagttggcaatgaaccatctataggtatcggagcggatcagagctacaaaatggaagcatcgaggaggctccgggacctgcaatcgcagccgggaaacaagatcaagatctgcatggactgctcacagaagaatccacaatgggcttcagtctcctacggcgtcttcatgtgcctagagtgctccggcaaccaccgccgtctcggcgtccacatctccttcgtcagatctgtgactagttcaaacatttttcagaaattcaatacagaccttagtgatctcggcgctggtgtcttcgtccggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

220

Amino Acids

24.09

Weight (kDa)

9.99

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 141 - 202 6.7e-23 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 273
AccBSI CCGCTC 1 cut(s) 392
AciI CCGC 2 cut(s) 392, 553
AclWI GGATC 2 cut(s) 185, 402
AcsI RAATTY 2 cut(s) 120, 614
AcuI CTGAAG 1 cut(s) 489
AcvI CACGTG 1 cut(s) 221
AcyI GRCGYC 2 cut(s) 519, 564
AdeI CACNNNGTG 1 cut(s) 221
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 2 cut(s) 273, 380
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 4 cut(s) 34, 88, 600, 619
AhlI ACTAGT 1 cut(s) 593
AjiI CACGTC 1 cut(s) 185
AluBI AGCT 1 cut(s) 402
AluI AGCT 1 cut(s) 402
Alw21I GWGCWC 1 cut(s) 542
Alw26I GTCTC 3 cut(s) 61, 514, 563
AlwI GGATC 2 cut(s) 185, 402
Ama87I CYCGRG 1 cut(s) 125
AoxI GGCC 2 cut(s) 101, 259
ApeKI GCWGC 1 cut(s) 445
ApoI RAATTY 2 cut(s) 120, 614
AspLEI GCGC 2 cut(s) 279, 644
AspS9I GGNCC 3 cut(s) 102, 260, 433
AsuC2I CCSGG 2 cut(s) 431, 450
AvaI CYCGRG 1 cut(s) 125
AvaII GGWCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 328
BbrPI CACGTG 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 514, 643
Bbv12I GWGCWC 1 cut(s) 542
BbvI GCAGC 1 cut(s) 457
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 532, 540
BcnI CCSGG 2 cut(s) 431, 450
BcoDI GTCTC 3 cut(s) 61, 514, 563
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 4 cut(s) 294, 300, 533, 594
BfmI CTRYAG 1 cut(s) 378
BfoI RGCGCY 1 cut(s) 645
BfuAI ACCTGC 1 cut(s) 444
BglI GCCNNNNNGGC 1 cut(s) 561
BglII AGATCT 2 cut(s) 465, 584
BisI GCNGC 1 cut(s) 446
BlsI GCNGC 1 cut(s) 447
Bme1390I CCNGG 2 cut(s) 431, 450
Bme18I GGWCC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 125
BmgBI CACGTC 1 cut(s) 185
BmgT120I GGNCC 3 cut(s) 102, 260, 433
BmiI GGNNCC 3 cut(s) 330, 428, 434
BmrFI CCNGG 2 cut(s) 431, 450
BmsI GCATC 1 cut(s) 425
BpiI GAAGAC 2 cut(s) 514, 643
BpuMI CCSGG 2 cut(s) 431, 450
BsaAI YACGTR 1 cut(s) 221
BsaBI GATNNNNATC 1 cut(s) 464
BsaHI GRCGYC 2 cut(s) 519, 564
BsaWI WCCGGW 1 cut(s) 657
Bsc4I CCNNNNNNNGG 2 cut(s) 273, 380
Bse3DI GCAATG 2 cut(s) 177, 373
Bse8I GATNNNNATC 1 cut(s) 464
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 2 cut(s) 273, 380
BseMI GCAATG 2 cut(s) 177, 373
BseMII CTCAG 1 cut(s) 54
BseRI GAGGAG 1 cut(s) 436
BseXI GCAGC 1 cut(s) 457
BshFI GGCC 2 cut(s) 103, 261
BshNI GGYRCC 1 cut(s) 328
BsiHKAI GWGCWC 1 cut(s) 542
BsiHKCI CYCGRG 1 cut(s) 125
BsiSI CCGG 4 cut(s) 430, 449, 543, 658
BslFI GGGAC 2 cut(s) 124, 446
BslI CCNNNNNNNGG 2 cut(s) 273, 380
BsmAI GTCTC 3 cut(s) 61, 514, 563
BsmBI CGTCTC 1 cut(s) 563
BsmFI GGGAC 2 cut(s) 124, 446
BsnI GGCC 2 cut(s) 103, 261
BsoBI CYCGRG 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 542
Bsp143I GATC 6 cut(s) 190, 394, 459, 465, 584, 634
BspACI CCGC 2 cut(s) 392, 553
BspANI GGCC 2 cut(s) 103, 261
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 3 cut(s) 330, 428, 434
BspMI ACCTGC 1 cut(s) 444
BspPI GGATC 2 cut(s) 185, 402
BspT107I GGYRCC 1 cut(s) 328
BsrBI CCGCTC 1 cut(s) 392
BsrDI GCAATG 2 cut(s) 177, 373
BssMI GATC 6 cut(s) 190, 394, 459, 465, 584, 634
BssNI GRCGYC 2 cut(s) 519, 564
BstACI GRCGYC 2 cut(s) 519, 564
BstAPI GCANNNNNTGC 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 221
BstDEI CTNAG 3 cut(s) 63, 333, 629
BstH2I RGCGCY 1 cut(s) 645
BstHHI GCGC 2 cut(s) 279, 644
BstKTI GATC 6 cut(s) 193, 397, 462, 468, 587, 637
BstMAI GTCTC 3 cut(s) 61, 514, 563
BstMBI GATC 6 cut(s) 190, 394, 459, 465, 584, 634
BstMWI GCNNNNNNNGC 6 cut(s) 258, 445, 477, 537, 552, 561
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 429, 448
BstSFI CTRYAG 1 cut(s) 378
BstV1I GCAGC 1 cut(s) 457
BstV2I GAAGAC 2 cut(s) 514, 643
BstX2I RGATCY 2 cut(s) 465, 584
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 2 cut(s) 465, 584
BsuRI GGCC 2 cut(s) 103, 261
BtrI CACGTC 1 cut(s) 185
BveI ACCTGC 1 cut(s) 444
CfoI GCGC 2 cut(s) 279, 644
Cfr13I GGNCC 3 cut(s) 102, 260, 433
CseI GACGC 2 cut(s) 508, 553
Csp6I GTAC 1 cut(s) 291
CviAII CATG 5 cut(s) 203, 233, 308, 472, 526
CviJI RGCY 9 cut(s) 103, 131, 252, 261, 297, 402, 427, 448, 503
CviKI_1 RGCY 9 cut(s) 103, 131, 252, 261, 297, 402, 427, 448, 503
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 3 cut(s) 63, 333, 629
DpnI GATC 6 cut(s) 192, 396, 461, 467, 586, 636
DpnII GATC 6 cut(s) 190, 394, 459, 465, 584, 634
DraIII CACNNNGTG 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 433
Eco57I CTGAAG 1 cut(s) 489
Eco72I CACGTG 1 cut(s) 221
Eco88I CYCGRG 1 cut(s) 125
EcoO109I RGGNCCY 2 cut(s) 260, 433
Esp3I CGTCTC 1 cut(s) 563
FaeI CATG 5 cut(s) 206, 236, 311, 475, 529
FaqI GGGAC 2 cut(s) 124, 446
FatI CATG 5 cut(s) 202, 232, 307, 471, 525
Fnu4HI GCNGC 1 cut(s) 446
Fsp4HI GCNGC 1 cut(s) 446
FspBI CTAG 4 cut(s) 294, 300, 533, 594
GlaI GCGC 2 cut(s) 278, 643
GluI GCNGC 1 cut(s) 446
HaeII RGCGCY 1 cut(s) 645
HaeIII GGCC 2 cut(s) 103, 261
HapII CCGG 4 cut(s) 430, 449, 543, 658
HgaI GACGC 2 cut(s) 508, 553
HhaI GCGC 2 cut(s) 279, 644
Hin1I GRCGYC 2 cut(s) 519, 564
Hin1II CATG 5 cut(s) 206, 236, 311, 475, 529
Hin6I GCGC 2 cut(s) 277, 642
HinP1I GCGC 2 cut(s) 277, 642
HinfI GANTC 3 cut(s) 21, 79, 490
HpaII CCGG 4 cut(s) 430, 449, 543, 658
Hpy166II GTNNAC 1 cut(s) 568
Hpy188I TCNGA 4 cut(s) 389, 399, 584, 612
Hpy188III TCNNGA 2 cut(s) 125, 463
Hpy8I GTNNAC 1 cut(s) 568
HpyAV CCTTC 2 cut(s) 351, 586
HpyCH4IV ACGT 2 cut(s) 184, 220
HpyCH4V TGCA 6 cut(s) 55, 170, 215, 322, 439, 471
HpyF10VI GCNNNNNNNGC 6 cut(s) 258, 445, 477, 537, 552, 561
HpyF3I CTNAG 3 cut(s) 63, 333, 629
HpySE526I ACGT 2 cut(s) 184, 220
Hsp92I GRCGYC 2 cut(s) 519, 564
Hsp92II CATG 5 cut(s) 206, 236, 311, 475, 529
HspAI GCGC 2 cut(s) 277, 642
Kzo9I GATC 6 cut(s) 190, 394, 459, 465, 584, 634
LmnI GCTCC 3 cut(s) 389, 432, 545
LpnPI CCDG 7 cut(s) 65, 85, 443, 449, 462, 556, 630
Lsp1109I GCAGC 1 cut(s) 457
LweI GCATC 1 cut(s) 425
MaeI CTAG 4 cut(s) 294, 300, 533, 594
MaeII ACGT 2 cut(s) 184, 220
MaeIII GTNAC 2 cut(s) 58, 589
MalI GATC 6 cut(s) 192, 396, 461, 467, 586, 636
MbiI CCGCTC 1 cut(s) 392
MboI GATC 6 cut(s) 190, 394, 459, 465, 584, 634
MboII GAAGA 4 cut(s) 169, 499, 514, 643
MflI RGATCY 2 cut(s) 465, 584
MhlI GDGCHC 1 cut(s) 542
MluCI AATT 3 cut(s) 120, 133, 614
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 6 cut(s) 59, 121, 251, 273, 414, 417
MspI CCGG 4 cut(s) 430, 449, 543, 658
MspR9I CCNGG 2 cut(s) 431, 450
MwoI GCNNNNNNNGC 6 cut(s) 258, 445, 477, 537, 552, 561
NciI CCSGG 2 cut(s) 431, 450
NdeII GATC 6 cut(s) 190, 394, 459, 465, 584, 634
NlaIII CATG 5 cut(s) 206, 236, 311, 475, 529
NlaIV GGNNCC 3 cut(s) 330, 428, 434
NmeAIII GCCGAG 2 cut(s) 540, 618
NmuCI GTSAC 2 cut(s) 58, 589
NspI RCATGY 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 125
PciI ACATGT 1 cut(s) 232
PfeI GAWTC 3 cut(s) 21, 79, 490
PflMI CCANNNNNTGG 1 cut(s) 273
PfoI TCCNGGA 1 cut(s) 429
PkrI GCNGC 1 cut(s) 447
PmaCI CACGTG 1 cut(s) 221
PmlI CACGTG 1 cut(s) 221
Ppu21I YACGTR 1 cut(s) 221
PpuMI RGGWCCY 1 cut(s) 433
PscI ACATGT 1 cut(s) 232
Psp5II RGGWCCY 1 cut(s) 433
PspCI CACGTG 1 cut(s) 221
PspN4I GGNNCC 3 cut(s) 330, 428, 434
PspPI GGNCC 3 cut(s) 102, 260, 433
PspPPI RGGWCCY 1 cut(s) 433
PsuI RGATCY 2 cut(s) 465, 584
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
SatI GCNGC 1 cut(s) 446
Sau3AI GATC 6 cut(s) 190, 394, 459, 465, 584, 634
Sau96I GGNCC 3 cut(s) 102, 260, 433
ScrFI CCNGG 2 cut(s) 431, 450
SduI GDGCHC 1 cut(s) 542
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 1 cut(s) 378
Sfr274I CTCGAG 1 cut(s) 125
SinI GGWCC 1 cut(s) 433
SlaI CTCGAG 1 cut(s) 125
SmlI CTYRAG 1 cut(s) 125
SmoI CTYRAG 1 cut(s) 125
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 3 cut(s) 120, 133, 614
SsiI CCGC 2 cut(s) 392, 553
SspMI CTAG 4 cut(s) 294, 300, 533, 594
StyD4I CCNGG 2 cut(s) 429, 448
TaiI ACGT 2 cut(s) 187, 223
TaqI TCGA 2 cut(s) 126, 419
TaqII GACCGA 1 cut(s) 334
TasI AATT 3 cut(s) 120, 133, 614
TfiI GAWTC 3 cut(s) 21, 79, 490
TseFI GTSAC 2 cut(s) 58, 589
TseI GCWGC 1 cut(s) 445
Tsp45I GTSAC 2 cut(s) 58, 589
TspDTI ATGAA 2 cut(s) 384, 514
Van91I CCANNNNNTGG 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 433
XapI RAATTY 2 cut(s) 120, 614
XceI RCATGY 1 cut(s) 236
XhoI CTCGAG 1 cut(s) 125
XspI CTAG 4 cut(s) 294, 300, 533, 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.