Rmu_sc0000759.1_g000014

synapsis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000759.1
OG0008767
Physical Location & Seq
Forward (+)
67778 .. 70511
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
atgcatggtctctttatagaaagtgctggggacgtggcgacctggtccagtcacgcgatccgcacctcaggccgttactataaattcactgcattccactccatttcctcaccatttcaaacacgtttcgtttctccaaaaatccagacaagcacagagcctgaaaaaaaaatggcgggcactctggctttgataccaagcaatccgccggtgacgtcatcgtctctgagctccatccgagagcggtgggaggtcgagttcgctcgcttcttcggctaccctccgctcacctccacgtgtccagatctcgtccctcttcctatgaaggtcaggaatcgacggccagtcggtagctggatctccgccgcctccgccgccatgcttcaactcatttatgaccactccagctccgacgtcatcctcaccgtctgctccagcggcaagatcatggaggagcactacgtgtcgaagctactcttttcctggcctcaggttgcatgcatgtctggatatcctgccaggggcagtagagccatctttgccagctacagagattctttgggccagatccagaaatttgcttttcgatttttgtcagtcaatgaagcagagaagttcataactcttatgaaggagattttcaaggaagggagggatattcaacctcttaatactgactatggatctgaaatttcatcacaatctgagttcatgtcttccaatagacctctaaacagaacttccaaggacttggttgtcatgactcctgttcacagtcattctcctcaaatatcaccaagcttaatgacggcttatactcctcaaatgtcaccaagcttaatgaaggattatacacctcaaatgtcaccaacctggaaacatgaagcagctcaacacagacaaacggagcacatgtcacctattcagaactttcagagcaactttgctgcctttcctcctagtttcacctcattagtgtccaactgtcaacctctggttgagcaagctccagcacaatcaactgcatcaaaggaagtcgatctcaaggccctaattgcgagatgtatggaagactcttccttccaagatatattgagccaagttgagacagttatgggcgaaatgggggctgatttgaagctgtag

Protein Analysis

381

Amino Acids

42.5

Weight (kDa)

7.7

Isoelectric Point (pI)

60.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PHS1 PF25349 81 - 223 2.9e-62 Poor homologous synapsis protein PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 220, 755
AatII GACGTC 2 cut(s) 218, 417
AccBSI CCGCTC 2 cut(s) 244, 286
AccII CGCG 1 cut(s) 56
AclWI GGATC 4 cut(s) 52, 365, 562, 691
AcoI YGGCCR 1 cut(s) 341
AcsI RAATTY 3 cut(s) 83, 575, 690
AcvI CACGTG 1 cut(s) 297
AcyI GRCGYC 2 cut(s) 215, 414
AdeI CACNNNGTG 1 cut(s) 463
AfiI CCNNNNNNNGG 1 cut(s) 521
AflIII ACRYGT 4 cut(s) 122, 296, 462, 912
AgsI TTSAA 5 cut(s) 119, 386, 643, 662, 1138
AhdI GACNNNNNGTC 1 cut(s) 344
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 4 cut(s) 41, 482, 518, 872
AloI GAACNNNNNNTCC 2 cut(s) 242, 274
Alw21I GWGCWC 3 cut(s) 233, 459, 912
Alw26I GTCTC 3 cut(s) 14, 228, 1100
AlwI GGATC 4 cut(s) 52, 365, 562, 691
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 5 cut(s) 70, 341, 485, 562, 1047
ApeKI GCWGC 2 cut(s) 887, 947
ApoI RAATTY 3 cut(s) 83, 575, 690
AspS9I GGNCC 3 cut(s) 45, 562, 1048
AsuHPI GGTGA 9 cut(s) 102, 223, 280, 415, 786, 822, 858, 909, 958
AvaII GGWCC 1 cut(s) 45
AxyI CCTNAGG 2 cut(s) 67, 489
BaeGI GKGCMC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 233
BbrPI CACGTG 1 cut(s) 297
BbsI GAAGAC 2 cut(s) 708, 1077
Bbv12I GWGCWC 3 cut(s) 233, 459, 912
BbvI GCAGC 2 cut(s) 899, 934
BccI CCATC 2 cut(s) 242, 542
BceAI ACGGC 3 cut(s) 57, 356, 825
BciT130I CCWGG 4 cut(s) 43, 484, 520, 874
BcoDI GTCTC 3 cut(s) 14, 228, 1100
BfaI CTAG 1 cut(s) 960
BfmI CTRYAG 2 cut(s) 547, 1142
BglII AGATCT 1 cut(s) 304
BisI GCNGC 5 cut(s) 366, 375, 439, 888, 948
BlsI GCNGC 5 cut(s) 367, 376, 440, 889, 949
Bme1390I CCNGG 4 cut(s) 43, 484, 520, 874
Bme18I GGWCC 1 cut(s) 45
BmeRI GACNNNNNGTC 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 3 cut(s) 45, 562, 1048
BmrFI CCNGG 4 cut(s) 43, 484, 520, 874
BmsI GCATC 1 cut(s) 1034
BpiI GAAGAC 2 cut(s) 708, 1077
BpmI CTGGAG 3 cut(s) 388, 418, 993
BpuEI CTTGAG 1 cut(s) 1028
BsaAI YACGTR 2 cut(s) 297, 463
BsaHI GRCGYC 2 cut(s) 215, 414
BsaI GGTCTC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 519, 744
BsaXI ACNNNNNCTCC 6 cut(s) 242, 272, 392, 422, 899, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 521
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 2 cut(s) 48, 344
Bse21I CCTNAGG 2 cut(s) 67, 489
BseBI CCWGG 4 cut(s) 43, 484, 520, 874
BseDI CCNNGG 2 cut(s) 519, 744
BseGI GGATG 2 cut(s) 234, 417
BseLI CCNNNNNNNGG 1 cut(s) 521
BseMII CTCAG 4 cut(s) 81, 218, 503, 696
BseNI ACTGG 2 cut(s) 48, 344
BseRI GAGGAG 3 cut(s) 467, 774, 810
BseSI GKGCMC 1 cut(s) 182
BseXI GCAGC 2 cut(s) 899, 934
BseYI CCCAGC 1 cut(s) 26
Bsh1236I CGCG 1 cut(s) 56
BshFI GGCC 5 cut(s) 72, 343, 487, 564, 1049
BsiHKAI GWGCWC 3 cut(s) 233, 459, 912
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 2 cut(s) 44, 296
BslI CCNNNNNNNGG 1 cut(s) 521
BsmAI GTCTC 3 cut(s) 14, 228, 1100
BsmBI CGTCTC 1 cut(s) 228
BsmFI GGGAC 2 cut(s) 44, 296
BsmI GAATGC 1 cut(s) 92
BsnI GGCC 5 cut(s) 72, 343, 487, 564, 1049
Bso31I GGTCTC 1 cut(s) 14
Bsp1286I GDGCHC 4 cut(s) 182, 233, 459, 912
Bsp143I GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
BspANI GGCC 5 cut(s) 72, 343, 487, 564, 1049
BspCNI CTCAG 4 cut(s) 80, 219, 502, 697
BspFNI CGCG 1 cut(s) 56
BspHI TCATGA 1 cut(s) 759
BspPI GGATC 4 cut(s) 52, 365, 562, 691
BspTNI GGTCTC 1 cut(s) 14
BsrBI CCGCTC 2 cut(s) 244, 286
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 2 cut(s) 48, 344
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 519, 744
BssMI GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
BssNI GRCGYC 2 cut(s) 215, 414
BssT1I CCWWGG 1 cut(s) 744
Bst2UI CCWGG 4 cut(s) 43, 484, 520, 874
Bst4CI ACNGT 4 cut(s) 427, 776, 986, 1111
Bst6I CTCTTC 2 cut(s) 321, 1081
BstACI GRCGYC 2 cut(s) 215, 414
BstBAI YACGTR 2 cut(s) 297, 463
BstC8I GCNNGC 5 cut(s) 178, 265, 499, 544, 1005
BstDEI CTNAG 4 cut(s) 67, 227, 489, 705
BstF5I GGATG 2 cut(s) 234, 417
BstFNI CGCG 1 cut(s) 56
BstKTI GATC 7 cut(s) 60, 307, 360, 447, 570, 686, 1042
BstMAI GTCTC 3 cut(s) 14, 228, 1100
BstMBI GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
BstMWI GCNNNNNNNGC 7 cut(s) 69, 273, 371, 374, 438, 539, 1055
BstNI CCWGG 4 cut(s) 43, 484, 520, 874
BstNSI RCATGY 3 cut(s) 501, 505, 916
BstSCI CCNGG 4 cut(s) 41, 482, 518, 872
BstSFI CTRYAG 2 cut(s) 547, 1142
BstSLI GKGCMC 1 cut(s) 182
BstUI CGCG 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 899, 934
BstV2I GAAGAC 2 cut(s) 708, 1077
BstX2I RGATCY 4 cut(s) 304, 357, 567, 683
BstXI CCANNNNNNTGG 1 cut(s) 751
BstYI RGATCY 4 cut(s) 304, 357, 567, 683
Bsu36I CCTNAGG 2 cut(s) 67, 489
BsuRI GGCC 5 cut(s) 72, 343, 487, 564, 1049
BtrI CACGTC 1 cut(s) 34
BtsCI GGATG 2 cut(s) 234, 417
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 5 cut(s) 178, 265, 499, 544, 1005
CaiI CAGNNNCTG 1 cut(s) 161
CciI TCATGA 1 cut(s) 759
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 3 cut(s) 45, 562, 1048
CsiI ACCWGGT 1 cut(s) 41
CviAII CATG 9 cut(s) 5, 379, 448, 498, 502, 712, 760, 881, 913
DdeI CTNAG 4 cut(s) 67, 227, 489, 705
DpnI GATC 7 cut(s) 59, 306, 359, 446, 569, 685, 1041
DpnII GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
DraIII CACNNNGTG 1 cut(s) 463
DrdI GACNNNNNNGTC 2 cut(s) 220, 755
DriI GACNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 2 cut(s) 220, 755
EaeI YGGCCR 1 cut(s) 341
Eam1104I CTCTTC 2 cut(s) 321, 1081
Eam1105I GACNNNNNGTC 1 cut(s) 344
EarI CTCTTC 2 cut(s) 321, 1081
EciI GGCGGA 3 cut(s) 195, 352, 361
Ecl136II GAGCTC 1 cut(s) 231
Eco130I CCWWGG 1 cut(s) 744
Eco24I GRGCYC 1 cut(s) 233
Eco31I GGTCTC 1 cut(s) 14
Eco32I GATATC 1 cut(s) 512
Eco47I GGWCC 1 cut(s) 45
Eco53kI GAGCTC 1 cut(s) 231
Eco72I CACGTG 1 cut(s) 297
Eco81I CCTNAGG 2 cut(s) 67, 489
EcoICRI GAGCTC 1 cut(s) 231
EcoO109I RGGNCCY 1 cut(s) 1048
EcoRII CCWGG 4 cut(s) 41, 482, 518, 872
EcoRV GATATC 1 cut(s) 512
EcoT14I CCWWGG 1 cut(s) 744
EcoT22I ATGCAT 2 cut(s) 6, 503
EcoT38I GRGCYC 1 cut(s) 233
ErhI CCWWGG 1 cut(s) 744
Esp3I CGTCTC 1 cut(s) 228
FaeI CATG 9 cut(s) 8, 382, 451, 501, 505, 715, 763, 884, 916
FalI AAGNNNNNCTT 2 cut(s) 461, 493
FaqI GGGAC 2 cut(s) 44, 296
FatI CATG 9 cut(s) 4, 378, 447, 497, 501, 711, 759, 880, 912
FauI CCCGC 1 cut(s) 169
Fnu4HI GCNGC 5 cut(s) 366, 375, 439, 888, 948
FokI GGATG 2 cut(s) 221, 404
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 5 cut(s) 366, 375, 439, 888, 948
FspBI CTAG 1 cut(s) 960
GluI GCNGC 5 cut(s) 366, 375, 439, 888, 948
GsaI CCCAGC 1 cut(s) 30
GsuI CTGGAG 3 cut(s) 388, 418, 993
HaeIII GGCC 5 cut(s) 72, 343, 487, 564, 1049
HapII CCGG 1 cut(s) 209
Hin1I GRCGYC 2 cut(s) 215, 414
Hin1II CATG 9 cut(s) 8, 382, 451, 501, 505, 715, 763, 884, 916
HincII GTYRAC 1 cut(s) 989
HindII GTYRAC 1 cut(s) 989
HindIII AAGCTT 2 cut(s) 799, 835
HinfI GANTC 4 cut(s) 334, 554, 763, 1073
HpaII CCGG 1 cut(s) 209
HphI GGTGA 9 cut(s) 102, 223, 280, 415, 786, 822, 858, 909, 958
Hpy166II GTNNAC 2 cut(s) 772, 989
Hpy188I TCNGA 7 cut(s) 228, 239, 412, 688, 706, 927, 936
Hpy188III TCNNGA 6 cut(s) 145, 302, 331, 507, 571, 760
Hpy8I GTNNAC 2 cut(s) 772, 989
Hpy99I CGWCG 2 cut(s) 342, 416
HpyAV CCTTC 5 cut(s) 319, 625, 641, 838, 1090
HpyCH4III ACNGT 4 cut(s) 427, 776, 986, 1111
HpyCH4IV ACGT 6 cut(s) 33, 124, 215, 296, 414, 462
HpyCH4V TGCA 5 cut(s) 4, 92, 497, 501, 1025
HpyF10VI GCNNNNNNNGC 7 cut(s) 69, 273, 371, 374, 438, 539, 1055
HpyF3I CTNAG 4 cut(s) 67, 227, 489, 705
HpySE526I ACGT 6 cut(s) 33, 124, 215, 296, 414, 462
Hsp92I GRCGYC 2 cut(s) 215, 414
Hsp92II CATG 9 cut(s) 8, 382, 451, 501, 505, 715, 763, 884, 916
Kzo9I GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
LmnI GCTCC 6 cut(s) 236, 413, 437, 454, 907, 1012
Lsp1109I GCAGC 2 cut(s) 899, 934
LweI GCATC 1 cut(s) 1034
MabI ACCWGGT 1 cut(s) 41
MaeI CTAG 1 cut(s) 960
MaeII ACGT 6 cut(s) 33, 124, 215, 296, 414, 462
MaeIII GTNAC 6 cut(s) 50, 74, 211, 828, 864, 915
MalI GATC 7 cut(s) 59, 306, 359, 446, 569, 685, 1041
MbiI CCGCTC 2 cut(s) 244, 286
MboI GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
MboII GAAGA 5 cut(s) 262, 308, 708, 1068, 1082
MflI RGATCY 4 cut(s) 304, 357, 567, 683
MhlI GDGCHC 4 cut(s) 182, 233, 459, 912
MluCI AATT 4 cut(s) 83, 575, 690, 1053
MlyI GAGTC 2 cut(s) 757, 1067
MmeI TCCRAC 2 cut(s) 435, 1005
Mph1103I ATGCAT 2 cut(s) 6, 503
MseI TTAA 3 cut(s) 669, 803, 839
MspA1I CMGCKG 1 cut(s) 438
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 4 cut(s) 43, 484, 520, 874
Mva1269I GAATGC 1 cut(s) 92
MvaI CCWGG 4 cut(s) 43, 484, 520, 874
MvnI CGCG 1 cut(s) 56
MwoI GCNNNNNNNGC 7 cut(s) 69, 273, 371, 374, 438, 539, 1055
NdeII GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
NlaIII CATG 9 cut(s) 8, 382, 451, 501, 505, 715, 763, 884, 916
NmuCI GTSAC 5 cut(s) 50, 211, 828, 864, 915
NsiI ATGCAT 2 cut(s) 6, 503
NspI RCATGY 3 cut(s) 501, 505, 916
PaeI GCATGC 1 cut(s) 501
PagI TCATGA 1 cut(s) 759
PciI ACATGT 1 cut(s) 912
PctI GAATGC 1 cut(s) 92
PfeI GAWTC 2 cut(s) 334, 554
PflFI GACNNNGTC 1 cut(s) 43
PkrI GCNGC 5 cut(s) 367, 376, 440, 889, 949
PleI GAGTC 2 cut(s) 757, 1067
PmaCI CACGTG 1 cut(s) 297
PmlI CACGTG 1 cut(s) 297
PpsI GAGTC 2 cut(s) 757, 1067
Ppu21I YACGTR 2 cut(s) 297, 463
PscI ACATGT 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 233
Psp6I CCWGG 4 cut(s) 41, 482, 518, 872
PspCI CACGTG 1 cut(s) 297
PspFI CCCAGC 1 cut(s) 26
PspGI CCWGG 4 cut(s) 41, 482, 518, 872
PspPI GGNCC 3 cut(s) 45, 562, 1048
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 4 cut(s) 304, 357, 567, 683
PsyI GACNNNGTC 1 cut(s) 43
SacI GAGCTC 1 cut(s) 233
SaqAI TTAA 3 cut(s) 669, 803, 839
SatI GCNGC 5 cut(s) 366, 375, 439, 888, 948
Sau3AI GATC 7 cut(s) 57, 304, 357, 444, 567, 683, 1039
Sau96I GGNCC 3 cut(s) 45, 562, 1048
SchI GAGTC 2 cut(s) 757, 1067
ScrFI CCNGG 4 cut(s) 43, 484, 520, 874
SduI GDGCHC 4 cut(s) 182, 233, 459, 912
SexAI ACCWGGT 1 cut(s) 41
SfaNI GCATC 1 cut(s) 1034
SfcI CTRYAG 2 cut(s) 547, 1142
SgrAI CRCCGGYG 1 cut(s) 208
SinI GGWCC 1 cut(s) 45
SmlI CTYRAG 1 cut(s) 1043
SmoI CTYRAG 1 cut(s) 1043
SphI GCATGC 1 cut(s) 501
Sse9I AATT 4 cut(s) 83, 575, 690, 1053
SspMI CTAG 1 cut(s) 960
SstI GAGCTC 1 cut(s) 233
StyD4I CCNGG 4 cut(s) 41, 482, 518, 872
StyI CCWWGG 1 cut(s) 744
TaaI ACNGT 4 cut(s) 427, 776, 986, 1111
TaiI ACGT 6 cut(s) 36, 127, 218, 299, 417, 465
TaqI TCGA 5 cut(s) 255, 337, 467, 586, 1038
TasI AATT 4 cut(s) 83, 575, 690, 1053
TauI GCSGC 3 cut(s) 368, 377, 441
TfiI GAWTC 2 cut(s) 334, 554
Tru1I TTAA 3 cut(s) 669, 803, 839
Tru9I TTAA 3 cut(s) 669, 803, 839
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 5 cut(s) 50, 211, 828, 864, 915
TseI GCWGC 2 cut(s) 887, 947
Tsp45I GTSAC 5 cut(s) 50, 211, 828, 864, 915
TspDTI ATGAA 8 cut(s) 338, 607, 618, 644, 684, 700, 857, 897
TspGWI ACGGA 1 cut(s) 920
TspRI CASTG 1 cut(s) 94
Tth111I GACNNNGTC 1 cut(s) 43
VpaK11BI GGWCC 1 cut(s) 45
XapI RAATTY 3 cut(s) 83, 575, 690
XceI RCATGY 3 cut(s) 501, 505, 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 351
XspI CTAG 1 cut(s) 960
ZraI GACGTC 2 cut(s) 216, 415
Zsp2I ATGCAT 2 cut(s) 6, 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.