Rmu_sc0000882.1_g000037

Endoplasmic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000882.1
OG0017752
Physical Location & Seq
Reverse (-)
189359 .. 193829
4471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
atgttgggcttcggtgaattggtttctcgctgccatagtcggcggagtgagttggtgtcacaagttcagcgtcgtcgtgctatggtcatggcggtgcaacggggttctgatcgggctaacctgatctgggttgggtcctttggatcctatctaggggagagtctgattggtagaggcagagctcacccatgccgtttcagcgatggcgtgttgaatgcagaggtccggcgactctgcatatgtgaatgcccagaaaacgaatttccagaaccttttaaaaggcgcttgcaccgtggccttacatctgacagtttggtttgtcaagaggaaaagcttcagggttctgttgaccgcacgttagacagtagagttttcagaggctggattgatacagataacccttggataattgatgatgagaatggtgagatgacaaatgtgaaccttctactaaaccctgaacgttacactggctacacaggtccatcggctagaaggatatgggatgctgtttactctgaaaattgcccaaaatgtgtgtttaatattctgttagatcactatttgtga

Protein Analysis

189

Amino Acids

21.67

Weight (kDa)

6.89

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERO1 PF04137 63 - 177 4.2e-18 Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 43, 92, 352
AclI AACGTT 1 cut(s) 463
AclWI GGATC 2 cut(s) 138, 151
AcsI RAATTY 1 cut(s) 260
AcuI CTGAAG 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 127, 153
AgsI TTSAA 1 cut(s) 214
AluBI AGCT 2 cut(s) 182, 334
AluI AGCT 2 cut(s) 182, 334
Alw21I GWGCWC 1 cut(s) 184
AlwI GGATC 2 cut(s) 138, 151
AlwNI CAGNNNCTG 1 cut(s) 381
AoxI GGCC 1 cut(s) 295
ApeKI GCWGC 1 cut(s) 30
ApoI RAATTY 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 1 cut(s) 285
AspS9I GGNCC 3 cut(s) 135, 223, 482
AsuHPI GGTGA 3 cut(s) 26, 176, 437
AvaII GGWCC 3 cut(s) 135, 223, 482
BamHI GGATCC 1 cut(s) 143
BanII GRGCYC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 197, 493
BceAI ACGGC 1 cut(s) 177
BfaI CTAG 2 cut(s) 152, 492
BfoI RGCGCY 1 cut(s) 286
BisI GCNGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 32
Bme18I GGWCC 3 cut(s) 135, 223, 482
BmgT120I GGNCC 3 cut(s) 135, 223, 482
BmiI GGNNCC 2 cut(s) 136, 145
BmsI GCATC 1 cut(s) 496
BsaJI CCNNGG 2 cut(s) 292, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 153
Bse1I ACTGG 1 cut(s) 475
BseDI CCNNGG 2 cut(s) 292, 401
BseGI GGATG 1 cut(s) 511
BseLI CCNNNNNNNGG 2 cut(s) 127, 153
BseNI ACTGG 1 cut(s) 475
BseXI GCAGC 1 cut(s) 17
BshFI GGCC 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 184
BsiSI CCGG 1 cut(s) 226
BslI CCNNNNNNNGG 2 cut(s) 127, 153
BsmI GAATGC 2 cut(s) 220, 251
BsnI GGCC 1 cut(s) 297
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 4 cut(s) 109, 123, 143, 556
BspACI CCGC 3 cut(s) 43, 92, 352
BspANI GGCC 1 cut(s) 297
BspLI GGNNCC 2 cut(s) 136, 145
BspPI GGATC 2 cut(s) 138, 151
BsrI ACTGG 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 292, 401
BssMI GATC 4 cut(s) 109, 123, 143, 556
BssT1I CCWWGG 1 cut(s) 401
Bst4CI ACNGT 3 cut(s) 293, 311, 365
BstC8I GCNNGC 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 286
BstHHI GCGC 1 cut(s) 285
BstKTI GATC 4 cut(s) 112, 126, 146, 559
BstMBI GATC 4 cut(s) 109, 123, 143, 556
BstMWI GCNNNNNNNGC 1 cut(s) 198
BstV1I GCAGC 1 cut(s) 17
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 1 cut(s) 297
BtgI CCRYGG 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 216
BtsCI GGATG 1 cut(s) 511
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 287
CaiI CAGNNNCTG 1 cut(s) 381
CfoI GCGC 1 cut(s) 285
Cfr13I GGNCC 3 cut(s) 135, 223, 482
CseI GACGC 1 cut(s) 59
CviAII CATG 2 cut(s) 88, 189
CviJI RGCY 8 cut(s) 9, 116, 182, 297, 334, 381, 474, 491
CviKI_1 RGCY 8 cut(s) 9, 116, 182, 297, 334, 381, 474, 491
DpnI GATC 4 cut(s) 111, 125, 145, 558
DpnII GATC 4 cut(s) 109, 123, 143, 556
DraI TTTAAA 1 cut(s) 277
EciI GGCGGA 1 cut(s) 58
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 401
Eco24I GRGCYC 1 cut(s) 184
Eco47I GGWCC 3 cut(s) 135, 223, 482
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 401
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 401
FaeI CATG 2 cut(s) 91, 192
FaiI YATR 7 cut(s) 36, 83, 89, 190, 239, 241, 502
FatI CATG 2 cut(s) 87, 188
FauNDI CATATG 1 cut(s) 239
Fnu4HI GCNGC 1 cut(s) 31
FokI GGATG 1 cut(s) 518
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 1 cut(s) 31
FspBI CTAG 2 cut(s) 152, 492
GlaI GCGC 1 cut(s) 284
GluI GCNGC 1 cut(s) 31
HaeII RGCGCY 1 cut(s) 286
HaeIII GGCC 1 cut(s) 297
HapII CCGG 1 cut(s) 226
HgaI GACGC 1 cut(s) 59
HhaI GCGC 1 cut(s) 285
Hin1II CATG 2 cut(s) 91, 192
Hin6I GCGC 1 cut(s) 283
HinP1I GCGC 1 cut(s) 283
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 2 cut(s) 160, 231
HpaII CCGG 1 cut(s) 226
HphI GGTGA 3 cut(s) 26, 176, 437
Hpy166II GTNNAC 3 cut(s) 349, 442, 514
Hpy188I TCNGA 5 cut(s) 109, 165, 307, 377, 520
Hpy188III TCNNGA 2 cut(s) 266, 323
Hpy8I GTNNAC 3 cut(s) 349, 442, 514
Hpy99I CGWCG 2 cut(s) 75, 78
HpyAV CCTTC 2 cut(s) 455, 489
HpyCH4III ACNGT 3 cut(s) 293, 311, 365
HpyCH4IV ACGT 2 cut(s) 356, 463
HpyCH4V TGCA 4 cut(s) 97, 218, 237, 289
HpyF10VI GCNNNNNNNGC 1 cut(s) 198
HpySE526I ACGT 2 cut(s) 356, 463
Hsp92II CATG 2 cut(s) 91, 192
HspAI GCGC 1 cut(s) 283
Kzo9I GATC 4 cut(s) 109, 123, 143, 556
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 496
MaeI CTAG 2 cut(s) 152, 492
MaeII ACGT 2 cut(s) 356, 463
MaeIII GTNAC 2 cut(s) 57, 464
MalI GATC 4 cut(s) 111, 125, 145, 558
MboI GATC 4 cut(s) 109, 123, 143, 556
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 4 cut(s) 17, 260, 408, 523
MlyI GAGTC 2 cut(s) 169, 225
MnlI CCTC 4 cut(s) 167, 214, 319, 371
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 2 cut(s) 276, 543
MslI CAYNNNNRTG 1 cut(s) 92
MspI CCGG 1 cut(s) 226
Mva1269I GAATGC 2 cut(s) 220, 251
MwoI GCNNNNNNNGC 1 cut(s) 198
NdeI CATATG 1 cut(s) 239
NdeII GATC 4 cut(s) 109, 123, 143, 556
NlaIII CATG 2 cut(s) 91, 192
NlaIV GGNNCC 2 cut(s) 136, 145
NmuCI GTSAC 1 cut(s) 57
PctI GAATGC 2 cut(s) 220, 251
PdmI GAANNNNTTC 1 cut(s) 333
PkrI GCNGC 1 cut(s) 32
PleI GAGTC 2 cut(s) 168, 225
PpsI GAGTC 2 cut(s) 168, 225
PpuMI RGGWCCY 1 cut(s) 135
Psp124BI GAGCTC 1 cut(s) 184
Psp1406I AACGTT 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 136, 145
PspPI GGNCC 3 cut(s) 135, 223, 482
PspPPI RGGWCCY 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 381
PsuI RGATCY 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 276, 543
SatI GCNGC 1 cut(s) 31
Sau3AI GATC 4 cut(s) 109, 123, 143, 556
Sau96I GGNCC 3 cut(s) 135, 223, 482
SchI GAGTC 2 cut(s) 169, 225
SduI GDGCHC 1 cut(s) 184
SetI ASST 9 cut(s) 123, 184, 225, 274, 336, 359, 447, 466, 484
SfaNI GCATC 1 cut(s) 496
SinI GGWCC 3 cut(s) 135, 223, 482
SmiMI CAYNNNNRTG 1 cut(s) 92
Sse9I AATT 4 cut(s) 17, 260, 408, 523
SsiI CCGC 3 cut(s) 43, 92, 352
SspI AATATT 1 cut(s) 547
SspMI CTAG 2 cut(s) 152, 492
SstI GAGCTC 1 cut(s) 184
StyI CCWWGG 1 cut(s) 401
TaaI ACNGT 3 cut(s) 293, 311, 365
TaiI ACGT 2 cut(s) 359, 466
TasI AATT 4 cut(s) 17, 260, 408, 523
Tru1I TTAA 2 cut(s) 276, 543
Tru9I TTAA 2 cut(s) 276, 543
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 1 cut(s) 30
Tsp45I GTSAC 1 cut(s) 57
TspRI CASTG 1 cut(s) 475
VpaK11BI GGWCC 3 cut(s) 135, 223, 482
XapI RAATTY 1 cut(s) 260
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 152, 492
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.