Rorug06G0256800

Endoplasmic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0017752
Physical Location & Seq
Forward (+)
40382000 .. 40382479
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0256800.1

Sequence Viewer

Length: 480 bp
ATGAACATGGATATATTGAGTTGGAATTGCAGGGGAATCCGTAATACAACTACCACTAGAGCTTTGAGGGATCTGATCACTCAAAATCACCCTCAAATAGTCTTTCTTTGTGAGACAAAAATCAGTGCGCTAAGAGATTTCAAAGCGCTACATAAAGAGTTAGGTTTTGTTCACTCTGAGGAAGTGCTGAGTGTTGGGCAGGCTGGCGGCTTGGCACTTTTCTGGTCAGAAGATGTTGACCTACACATTCGTACCTTTTCTGCGCATCACATCGATGCAGAAGTTAGGGCAGGACCTGGTGAACCGTGGTGGAGACTAATAGGCTTCTACGGTTATGCATGCACCGGTGAACGTGGGGAGTCTTGGCAACTCCTACGTGATTTGGCTGATTTAGACTCTCTTCTATGGATAATAATCGGCGATTTCAATGAGATATGGAATAATAGTGAGAAGCTGGCTGGGAAGGCCTTTGCGGCCTGA

Protein Analysis

159

Amino Acids

18.03

Weight (kDa)

5.45

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 147 1.1e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 264
AciI CCGC 2 cut(s) 207, 473
AclWI GGATC 1 cut(s) 78
AfaI GTAC 1 cut(s) 253
AfeI AGCGCT 1 cut(s) 147
AgeI ACCGGT 1 cut(s) 344
AgsI TTSAA 2 cut(s) 142, 427
AjnI CCWGG 1 cut(s) 295
AluBI AGCT 2 cut(s) 62, 454
AluI AGCT 2 cut(s) 62, 454
Alw26I GTCTC 2 cut(s) 107, 307
AlwI GGATC 1 cut(s) 78
AlwNI CAGNNNCTG 1 cut(s) 296
Aor51HI AGCGCT 1 cut(s) 147
AoxI GGCC 2 cut(s) 465, 474
AsiGI ACCGGT 1 cut(s) 344
AspLEI GCGC 3 cut(s) 130, 148, 265
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 80, 311, 359
AvaII GGWCC 1 cut(s) 293
BaeI ACNNNNGTAYC 2 cut(s) 235, 268
BciT130I CCWGG 1 cut(s) 297
BclI TGATCA 1 cut(s) 75
BcoDI GTCTC 2 cut(s) 107, 307
BfaI CTAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 2 cut(s) 208, 474
BlsI GCNGC 2 cut(s) 209, 475
Bme1390I CCNGG 1 cut(s) 297
Bme18I GGWCC 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 293
BmrFI CCNGG 1 cut(s) 297
BmsI GCATC 2 cut(s) 265, 274
Bsa29I ATCGAT 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 305
BsaWI WCCGGW 1 cut(s) 344
Bse118I RCCGGY 1 cut(s) 344
Bse8I GATNNNNATC 1 cut(s) 413
BseBI CCWGG 1 cut(s) 297
BseCI ATCGAT 1 cut(s) 273
BseDI CCNNGG 1 cut(s) 305
BseJI GATNNNNATC 1 cut(s) 413
BseMII CTCAG 2 cut(s) 168, 179
BseYI CCCAGC 1 cut(s) 458
BshFI GGCC 2 cut(s) 467, 476
BshTI ACCGGT 1 cut(s) 344
BshVI ATCGAT 1 cut(s) 273
BsiSI CCGG 1 cut(s) 345
BsmAI GTCTC 2 cut(s) 107, 307
BsnI GGCC 2 cut(s) 467, 476
Bsp143I GATC 2 cut(s) 70, 75
BspACI CCGC 2 cut(s) 207, 473
BspANI GGCC 2 cut(s) 467, 476
BspCNI CTCAG 2 cut(s) 169, 180
BspDI ATCGAT 1 cut(s) 273
BspPI GGATC 1 cut(s) 78
BsrFI RCCGGY 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 1 cut(s) 305
BssMI GATC 2 cut(s) 70, 75
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 2 cut(s) 306, 332
Bst6I CTCTTC 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 4 cut(s) 201, 205, 340, 456
BstDEI CTNAG 3 cut(s) 131, 177, 188
BstDSI CCRYGG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 149
BstHHI GCGC 3 cut(s) 130, 148, 265
BstKTI GATC 2 cut(s) 73, 78
BstMAI GTCTC 2 cut(s) 107, 307
BstMBI GATC 2 cut(s) 70, 75
BstMWI GCNNNNNNNGC 2 cut(s) 464, 473
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 70
BstYI RGATCY 1 cut(s) 70
Bsu15I ATCGAT 1 cut(s) 273
BsuRI GGCC 2 cut(s) 467, 476
BsuTUI ATCGAT 1 cut(s) 273
BtgI CCRYGG 1 cut(s) 305
BtsIMutI CAGTG 1 cut(s) 130
Cac8I GCNNGC 4 cut(s) 201, 205, 340, 456
CaiI CAGNNNCTG 1 cut(s) 296
CfoI GCGC 3 cut(s) 130, 148, 265
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 273
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 252
CspAI ACCGGT 1 cut(s) 344
CviAII CATG 2 cut(s) 7, 339
CviJI RGCY 9 cut(s) 62, 203, 210, 324, 386, 454, 458, 467, 476
CviKI_1 RGCY 9 cut(s) 62, 203, 210, 324, 386, 454, 458, 467, 476
CviQI GTAC 1 cut(s) 252
DdeI CTNAG 3 cut(s) 131, 177, 188
DpnI GATC 2 cut(s) 72, 77
DpnII GATC 2 cut(s) 70, 75
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco147I AGGCCT 1 cut(s) 467
Eco47I GGWCC 1 cut(s) 293
Eco47III AGCGCT 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 340
FaeI CATG 2 cut(s) 10, 342
FaiI YATR 7 cut(s) 8, 14, 153, 336, 340, 406, 436
FatI CATG 2 cut(s) 6, 338
FbaI TGATCA 1 cut(s) 75
Fnu4HI GCNGC 2 cut(s) 208, 474
Fsp4HI GCNGC 2 cut(s) 208, 474
FspBI CTAG 1 cut(s) 57
FspI TGCGCA 1 cut(s) 264
GlaI GCGC 3 cut(s) 129, 147, 264
GluI GCNGC 2 cut(s) 208, 474
GsaI CCCAGC 1 cut(s) 462
HaeII RGCGCY 1 cut(s) 149
HaeIII GGCC 2 cut(s) 467, 476
HapII CCGG 1 cut(s) 345
HhaI GCGC 3 cut(s) 130, 148, 265
Hin1II CATG 2 cut(s) 10, 342
Hin6I GCGC 3 cut(s) 128, 146, 263
HinP1I GCGC 3 cut(s) 128, 146, 263
HincII GTYRAC 1 cut(s) 238
HindII GTYRAC 1 cut(s) 238
HinfI GANTC 3 cut(s) 36, 359, 395
HpaII CCGG 1 cut(s) 345
HphI GGTGA 3 cut(s) 80, 311, 359
Hpy166II GTNNAC 4 cut(s) 172, 238, 302, 350
Hpy188I TCNGA 3 cut(s) 75, 178, 229
Hpy8I GTNNAC 4 cut(s) 172, 238, 302, 350
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 2 cut(s) 306, 332
HpyCH4IV ACGT 2 cut(s) 352, 376
HpyCH4V TGCA 4 cut(s) 30, 278, 338, 342
HpyF10VI GCNNNNNNNGC 2 cut(s) 464, 473
HpyF3I CTNAG 3 cut(s) 131, 177, 188
HpySE526I ACGT 2 cut(s) 352, 376
Hsp92II CATG 2 cut(s) 10, 342
HspAI GCGC 3 cut(s) 128, 146, 263
Ksp22I TGATCA 1 cut(s) 75
Kzo9I GATC 2 cut(s) 70, 75
LweI GCATC 2 cut(s) 265, 274
MabI ACCWGGT 1 cut(s) 295
MaeI CTAG 1 cut(s) 57
MaeII ACGT 2 cut(s) 352, 376
MalI GATC 2 cut(s) 72, 77
MboI GATC 2 cut(s) 70, 75
MboII GAAGA 2 cut(s) 242, 392
MflI RGATCY 1 cut(s) 70
MluCI AATT 1 cut(s) 25
MlyI GAGTC 2 cut(s) 368, 389
MnlI CCTC 3 cut(s) 60, 102, 172
Mph1103I ATGCAT 1 cut(s) 340
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 1 cut(s) 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 464, 473
NdeII GATC 2 cut(s) 70, 75
NlaIII CATG 2 cut(s) 10, 342
NsbI TGCGCA 1 cut(s) 264
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 342
PaeI GCATGC 1 cut(s) 342
PceI AGGCCT 1 cut(s) 467
PfeI GAWTC 1 cut(s) 36
PinAI ACCGGT 1 cut(s) 344
PkrI GCNGC 2 cut(s) 209, 475
PleI GAGTC 2 cut(s) 367, 389
PpsI GAGTC 2 cut(s) 367, 389
Ppu21I YACGTR 1 cut(s) 377
PpuMI RGGWCCY 1 cut(s) 293
Psp5II RGGWCCY 1 cut(s) 293
Psp6I CCWGG 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 458
PspGI CCWGG 1 cut(s) 295
PspPI GGNCC 1 cut(s) 293
PspPPI RGGWCCY 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 253
RsaNI GTAC 1 cut(s) 252
RseI CAYNNNNRTG 1 cut(s) 273
SatI GCNGC 2 cut(s) 208, 474
Sau3AI GATC 2 cut(s) 70, 75
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 2 cut(s) 368, 389
ScrFI CCNGG 1 cut(s) 297
SetI ASST 8 cut(s) 64, 166, 243, 257, 298, 355, 379, 456
SexAI ACCWGGT 1 cut(s) 295
SfaNI GCATC 2 cut(s) 265, 274
SfiI GGCCNNNNNGGCC 1 cut(s) 473
SgrAI CRCCGGYG 1 cut(s) 344
SinI GGWCC 1 cut(s) 293
SmiMI CAYNNNNRTG 1 cut(s) 273
SphI GCATGC 1 cut(s) 342
Sse9I AATT 1 cut(s) 25
SseBI AGGCCT 1 cut(s) 467
SsiI CCGC 2 cut(s) 207, 473
SspMI CTAG 1 cut(s) 57
StuI AGGCCT 1 cut(s) 467
StyD4I CCNGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 306, 332
TaiI ACGT 2 cut(s) 355, 379
TaqI TCGA 1 cut(s) 273
TasI AATT 1 cut(s) 25
TauI GCSGC 2 cut(s) 210, 476
TfiI GAWTC 1 cut(s) 36
TscAI CASTG 1 cut(s) 130
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 293
XceI RCATGY 1 cut(s) 342
XspI CTAG 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.