Rh5AG086600

Endoplasmic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0017752
Physical Location & Seq
Forward (+)
7644671 .. 7646192
1522 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG086600.1

Sequence Viewer

Length: 390 bp
ATGAATTTCTGGGTTGAAGATATTGGAATTTGGTTTTCAATTGAAGTCTCGAGCTTTTGGTCAACTGGTCTTGCTCTTGGACTCTTGGTTCGATTCAAAAATCTTCAAGCTTGGCCAAGCTGTGGTGTGACTGTCCCTTCTGGACTGAAGATGGGGTGTGTTGACCGCACTCTAGACAGTAGGGCTTTCAGAGGCTGGATTGAGACAGATAACCCTTGGACAAATGATGATGAGACTGATAATGATGAGATAACATATGTGAACCTTCTACTGAACCCTGAACGTTACACTGGCTATGCTGGTCCATCAGCAAGAAGGATATGGGATGCTGTTTATTCTGAAAATTGCCCAAAATGTGAGTCGAAACTTTTGGTAGATCTACTTGGTTGA

Protein Analysis

129

Amino Acids

14.63

Weight (kDa)

4.45

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERO1 PF04137 68 - 118 1.6e-17 Endoplasmic Reticulum Oxidoreductin 1 (ERO1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 122
AciI CCGC 1 cut(s) 166
AclI AACGTT 1 cut(s) 283
AcoI YGGCCR 1 cut(s) 113
AcsI RAATTY 2 cut(s) 4, 27
AcuI CTGAAG 1 cut(s) 167
AfiI CCNNNNNNNGG 1 cut(s) 122
AgsI TTSAA 5 cut(s) 17, 39, 44, 97, 107
AloI GAACNNNNNNTCC 2 cut(s) 72, 104
AluBI AGCT 3 cut(s) 54, 110, 120
AluI AGCT 3 cut(s) 54, 110, 120
Alw26I GTCTC 3 cut(s) 52, 197, 227
AlwNI CAGNNNCTG 1 cut(s) 195
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 1 cut(s) 113
ApoI RAATTY 2 cut(s) 4, 27
ArsI GACNNNNNNTTYG 4 cut(s) 72, 104, 344, 376
AspS9I GGNCC 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 302
BalI TGGCCA 1 cut(s) 115
BccI CCATC 2 cut(s) 145, 313
BcoDI GTCTC 3 cut(s) 52, 197, 227
BfaI CTAG 1 cut(s) 173
BglII AGATCT 1 cut(s) 376
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 1 cut(s) 302
BmsI GCATC 1 cut(s) 316
BsaJI CCNNGG 1 cut(s) 215
Bsc4I CCNNNNNNNGG 1 cut(s) 122
Bse1I ACTGG 2 cut(s) 70, 295
BseDI CCNNGG 1 cut(s) 215
BseGI GGATG 1 cut(s) 331
BseLI CCNNNNNNNGG 1 cut(s) 122
BseNI ACTGG 2 cut(s) 70, 295
BshFI GGCC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 1 cut(s) 122
BsmAI GTCTC 3 cut(s) 52, 197, 227
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 49
Bsp143I GATC 1 cut(s) 376
BspACI CCGC 1 cut(s) 166
BspANI GGCC 1 cut(s) 115
BsrI ACTGG 2 cut(s) 70, 295
BssECI CCNNGG 1 cut(s) 215
BssMI GATC 1 cut(s) 376
BssT1I CCWWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 133, 179
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 1 cut(s) 379
BstMAI GTCTC 3 cut(s) 52, 197, 227
BstMBI GATC 1 cut(s) 376
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 1 cut(s) 115
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 1 cut(s) 288
CaiI CAGNNNCTG 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 302
CviJI RGCY 7 cut(s) 54, 110, 115, 120, 185, 195, 294
CviKI_1 RGCY 7 cut(s) 54, 110, 115, 120, 185, 195, 294
DpnI GATC 1 cut(s) 378
DpnII GATC 1 cut(s) 376
EaeI YGGCCR 1 cut(s) 113
Eco130I CCWWGG 1 cut(s) 215
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 49
EcoT14I CCWWGG 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 215
FaiI YATR 4 cut(s) 256, 258, 297, 322
FaqI GGGAC 1 cut(s) 119
FauNDI CATATG 1 cut(s) 256
FokI GGATG 1 cut(s) 338
FspBI CTAG 1 cut(s) 173
HaeIII GGCC 1 cut(s) 115
HincII GTYRAC 2 cut(s) 63, 163
HindII GTYRAC 2 cut(s) 63, 163
HindIII AAGCTT 1 cut(s) 108
HinfI GANTC 3 cut(s) 81, 93, 359
Hpy166II GTNNAC 3 cut(s) 63, 163, 262
Hpy188I TCNGA 2 cut(s) 191, 340
Hpy188III TCNNGA 3 cut(s) 49, 141, 173
Hpy8I GTNNAC 3 cut(s) 63, 163, 262
HpyAV CCTTC 3 cut(s) 147, 275, 309
HpyCH4III ACNGT 2 cut(s) 133, 179
HpyCH4IV ACGT 1 cut(s) 283
HpySE526I ACGT 1 cut(s) 283
Kzo9I GATC 1 cut(s) 376
LpnPI CCDG 6 cut(s) 51, 126, 181, 276, 285, 291
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 173
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 2 cut(s) 127, 284
MalI GATC 1 cut(s) 378
MboI GATC 1 cut(s) 376
MboII GAAGA 3 cut(s) 29, 95, 160
MfeI CAATTG 1 cut(s) 39
MflI RGATCY 1 cut(s) 376
MlsI TGGCCA 1 cut(s) 115
MluCI AATT 4 cut(s) 4, 27, 39, 343
MluNI TGGCCA 1 cut(s) 115
MlyI GAGTC 2 cut(s) 75, 368
MnlI CCTC 1 cut(s) 185
Mox20I TGGCCA 1 cut(s) 115
MscI TGGCCA 1 cut(s) 115
Msp20I TGGCCA 1 cut(s) 115
MunI CAATTG 1 cut(s) 39
NdeI CATATG 1 cut(s) 256
NdeII GATC 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 127
PaeR7I CTCGAG 1 cut(s) 49
PfeI GAWTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 122
PleI GAGTC 2 cut(s) 75, 367
PpsI GAGTC 2 cut(s) 75, 367
Psp1406I AACGTT 1 cut(s) 283
PspPI GGNCC 1 cut(s) 302
PstNI CAGNNNCTG 1 cut(s) 195
PsuI RGATCY 1 cut(s) 376
Sau3AI GATC 1 cut(s) 376
Sau96I GGNCC 1 cut(s) 302
SchI GAGTC 2 cut(s) 75, 368
SetI ASST 5 cut(s) 56, 112, 122, 267, 286
SfaNI GCATC 1 cut(s) 316
Sfr274I CTCGAG 1 cut(s) 49
SinI GGWCC 1 cut(s) 302
SlaI CTCGAG 1 cut(s) 49
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
Sse9I AATT 4 cut(s) 4, 27, 39, 343
SsiI CCGC 1 cut(s) 166
SspMI CTAG 1 cut(s) 173
StyI CCWWGG 1 cut(s) 215
TaaI ACNGT 2 cut(s) 133, 179
TaiI ACGT 1 cut(s) 286
TaqI TCGA 3 cut(s) 50, 91, 362
TasI AATT 4 cut(s) 4, 27, 39, 343
TfiI GAWTC 1 cut(s) 93
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 2 cut(s) 4, 27
XbaI TCTAGA 1 cut(s) 172
XhoI CTCGAG 1 cut(s) 49
XspI CTAG 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.