Rmu_sc0000894.1_g000061

Small subunit processome component 20 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000894.1
OG0005459
Physical Location & Seq
Reverse (-)
291865 .. 307514
15650 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 7881 bp
atggcaacctcttcacaagctcaggccgtcaagtccctcaacaaatccgccggccgccgccgctttgtcttcaagagcgtctctgagagactagaagaagtcgaaatcgatgtcttccgaagcctcgataagctaaaggacgagccttccccaggctccacgtttttccgggactgcctcgtagaatggagggaattgaataccgcagaggatttcatctcattccacgagcagatgacgccgttggtccagacattgcctttgatattgttgcacaaggagaccattttcgaagcattggtttctagattgcaaatcagtgcaaggttatcgcttgagcctatactcaggttgattgcagctctgtcccgagatctcctggaggactttatcctcttcttgccaaggattgtcgactctctagtgtctcttttagatagcggtgctgatagggagcctgagatcgtggagcagatctttacatcctggtcgtgcattatgagggatttgcagaaatatcttgtgcacaagcttgtagatatgctagaggtgactgcaaagttaagatattatccaaagaattatgtccaagaattaatggcacaggcgatgggattcttgctgagaacgagaactgcaccttttgagcagcttgacaaaggtatcagaaagatcatgcttgatgttgtggaggaatcaacacctttcagaaaatatggagtcagtgctttactatattataccatgacgggaacctcaacaaggttccattctagagccaagcaagtgttacttttattgatggacaactcaatacttggttcagccactgttattgaagtcttaatttctgcgttacagagattgtgtgaggatttggactccaaggagttaaacttgatgttcgaatgcttatatcaggaaataaagggctgtgtgactaatggaggtgtactgcgtttaagccgactgttgtcactgcttgtgtctactgttcaggtgaagaatgggaaagcggtttctgattacaaacaaatgcttgaaattgtgggggtgcttgtgcggacatatatcataccctctggcattcagatgggagaggaacatttatctgaagtcgttgataagatttttcagttaatgctcagcattcttagcggactgcacacttataatgatttgtcaaccatctctggctgttcgttgcaatgggctcctgtatttgatttacagaagtcaagcttgttggggtttattcaacaattgctacaaaaggatatttgtatagtagataccttccgagttaacattttaagagtcatgaatgttgtaatagaaacttcagaagaagatgttatatatctgttagtcatcttctgtgaaaggctgcaaaggggagcgcaaagctttaagttactagatggatctcctgaactgtccaggatccaatgttttctgagtggcacggtcaataaatgggttggggttttaaagggtgttcggaatggggatttagcatctacttcaattcatgaagctgacttggccctgctgtggggaatcattaactgctttccgcaaatagtagactccaaagaaggtccatccttgttacttgatttgacagatgcaatcgatcaactcctgatgattgaagatgggaatattgcaggctttcctaaacacacttggcaaagcctaattggcacttccttaaattcctattacaaattgacttgtggtaaaaatttggagccagaaacaagcagatttttagctcttggaaaaagacacaaatcatgttcacatgtgttggttgctgttgctgattttttggactctttgtatgggtctgcgatggatggcgacacccagtttacaatatatcatccagaacttagtgcatatatggctatggatgctttaaagatgtttgctgacaatttgtgccactcagacagagggattcgtgcttcaactcttagaatattatgccactttgaaactttaagttgcgatgagccagttctgaaaaagatgaaaactgaactttctcctacttctcatgtcgatagcaaagactttaatgttcttcagcttcttctgtcaatcgagtcaactcctctttcaatttctaccagcagaaaagttactctattgatttctagaatacagatgggcctctctgctggaaaaatatctgaagcttatcttccccttgttgtaaatagcatgattggtatcttccataaccggtttagtcatctttggaatcctgtatcagagtgccttgcagttctgataagccgaagtaatggacttgtatgggaaaattttctcaactattttgagcaatgccagtccttatttcaagcatctattgatcatgttggccaaggtgatactatgctgtccaacaagtcaagtgatctggttgaacgttttaatttgtttgctaccccaacatctgatagtacaccaacggcaacagttttgtcttccttgctccaatctttacaaaaacttccaatcattgaatccaagtctcatcagattctgcctttatttctgaaatttttgggctataattgtgaaaattttgtaagtgtgggatctttcaattcaaatgtttgtaaagggaaagagtggaaacgtgtccttaaagaatggttgaacttgctgaaactgatgtacaaccttaagcactcatatcagaatcagtttcttaaagaggttttgcaaaatagactcttggatgaaaatgatgctgaagtacagatgaaggttctcgattgcctcttaatctggaaagatgattccctgctcccatatagtcagcaattgaaaaacttggtcagcttccaaaatttaagagaagaattaacaagatggagtttgtccagagagtctaatctaattgaagaacaacacagagcttaccttgtgcccattgttatccgtcttctgattcccagcgtccggaaattgaagaaacatgcttctcagaagcactcaagagtaaatcatcgcaaggcagtgctcggtttcatagctcaacttgatgttgaagagctacctcttttctttgcattgttgataaagcccttgcaaattatttcgataggaagtgaaggtgctgcaaactggttttggagctcatcaaatggttctgtggaagaatttcaaacattgaatttcttgaagtatttcacgtttagtaatattacagccttgtcttggaagaaaagaagtgcttttctacatgtgattgaagatgttcttggagtttttgatgcatcacgtgttggtcccttccttgatttcataatgggatgtgttgttcggatattaggaagcggtactttaggtcttgatgtggcaaaaggcaaaggatcttcttcccttactaattattctgatgctaatcttgcttcccttggatatgatggggcagtggataataatgttttgatatctacggctgtggggcagttgaaggatttgagatctttatccttgaaaattgtgtcctttgttctgaataagtacgaagatcacgactttagctgtgaattctgggatcttttcttcgggtcggtgaagcccttaattgatggtttcaagcaggagggattcagtggccagaagcctagttcactcttctcttgctttcttgctatgtctagaagtgaaaaattagtatcactgctgtgtagggagcaaaaccttgtccctgatatactctcaattctaaccgtcacgtcagcatctgaagctattgtggcttgtgttctgaactttgttgagaacttgttgattcttgatgatgaattgggtgttgaagacaatgctggaaaaagagttatacttctgtaccttgaagcactagttgataacttgcattgtctcttcaaaaataatgttgcagccaagaggaaattgcttaaacacccaggggagacagaagtgaggatttttcaaatattaccgaagtatataagtgatgcattgtcagcctggaaattcgtggatatattgctccctgttttagcaaatggagctcaagattcagaatttcgatttgaagctgtgcaagtcatttgtgatattgttccagtactagggagtgacgttacaaataatattttgaatgcagtatctcccttacttatttctaccgatctagataagcgtttagttatctgtaatcttctggacacacttgccagagcagactcttccattcaatttgtggcaaaacttgtccaggacttgaatgcaacctctatcacggaaatagatagcgttgattatgatagagtgctcaatgcttatggaaagatcacggttaatatgttcaataccattcgagaggatcatgcattggtcatattgtctcactgtgtctatgacatgtcatctgacgaatcaaccttgaggcaccgagcttatgattcattgctttcatttgttgaattttctgccctcgtacttggcaaagtattgaacaatgactctgaaatgcctgacgagatgttagcctctgaggatcattgctggacaaaagtttgcattcagcgcatagaatgggtcgagttacttagagaaatggtgttaaaacttccagaagtggcaaaccttggttcactgaaacctcttcaggatgaaaatcttgaaatagattttttcaagaatatccttcatatacagaaacataggagagcaagggcaatgaaacgctttaagaattttgtcacaaacagctatatgccagagggcatcacaaagaaactgtttgttcccttcttcttcacgatcttgatggaggaagaaaaaggagagcatataaaaaatatgtgcatagaggtgattgcttcaatttcaagcagggagtggagttcatcctacttgttgctgatgagatgcttcaatgagatttccaaaaatcctctgaaacagaagcttcttttgcggctaatttgctctatcttgcaccagttccacttttcagagacaattgatactgcctctgtaaatgaaacacaaacatgccttcataaaactgtgcttcctaagatacaaaagttactcagcgattctgaaaaggtcagtgttaacatcagtcttgctgcactgagagtattaaagttgctacctggagatgtaatggactcgcagcttcctagcattattcatcgcatttcaaactttttgaagaacagattagaaagcatccgtgatgaagctaggagtgctttggctgattgtttgaaggagcttgggttggaatacttgcattttatagtcaaagttttgcgaagcactttaaaacgtggatttgagttgcatgtcttgggatatactctaaactttatcttatcaaagtttctcgttactcctatcagcgggaaactggactactgtttggaagatctgctttctattgcacagaatgatattcttggagatgttgctgaggaaaaagaggttgagaagattgcttccaaaatgaaagaaacaaagaagcaaaagtcttttgaaactctaaaattgattgctcaaagcataacattcaaaagccatgccttgaagcttctatcacctgttataactcaatttgagaaacatcttacaccaaaaacaaagtcaaaattggaaagtatgttgaatcacatagcagctggtattgagtgtaatccaactgttgatcagactgacctttttatatttgtacatggcctgattgaagatggaataaaagaagaaaatggtaagggtgagacttcgttcattacggggggaaatggacatcgtcgaagggatgtgattggaaaagttgattcctcaggccgcattgcaggtgctaaatcagtgtgttcacatcttatttctgtgtttgctcttgggatgttgcagaaacgcataaagaatttgaaagtgagaaaaaatgatgtacaaatattgtcgatgttggatccttttgtcatactgttagggaagtgcttgaattccaagtatgaagatgtcttatctgcaacacttagatgcctaacttcgttggtcaggttgcatttacctgccattgaagatcaagcagatagtataaaagcagtgctctttgatattgctcatagttggttaaatactggcagttctctaatggagtcatgtctgaggctgttaactgtacttttgcggggcaacaaaattactctttcctccgatcatctacatctattgattcagcttccagtgttcgttgatattgaaagggacccctcatttgttgctctttcacttctgaaggcaattgttaaacgcaagctagttgttccggagatatatgatcttgttactagagtctcagaattgatggtaacaagtcaagtagaaccaattcgtcacaaatgtagtcagattctgtctcacttcttaaatgattatcctctttcagaaaaacgattgcaacaacatttggattttttgctgtctaatctgaggtatgagcactctagtggaagaaaaactgtgcttgaaatgctccatgcagttatcgttaaaaatccacgaaatgttgtagatgagcaatcacagacgttttttgtccatttggtggtttgcttggctaatgatcgggataatgaagttcgtttgatggctggtgcggctataaaacgtctcactggttgtgtaagcccacgctcacttctctccattcttgagtacagtctatcttggtatttgggtgagaagcagcaactgtggggtgcagcagcacaggttttggggttgctggttgaagttgaagtcatgaagaaacgattccacaagcatatcaacagtgtattaaaagtgactaaaagcatcttgcagtctgccatcgatgctgtcactcatgattctcctcatgaaactgccattcctttctggaaagaggcattttattcattggatatatgggaagcaatttgtgagttactcctgcatccacacatgtggttacgttgtatttcaagtcgtcttatagccttctactttgcttccgtaaaagagtcttgctcaggagatcatgaaaatccatttggcacttactaccttataaaacctaataaacttttcatgatagctgcatatatctgctgccaactgaagacacaactcattgatgatgcagccaacaaactgattacacaaaatcttgcattcactgtgtgtggtgtgcactctttgatgggacaaacagagtgtgcagattctcatcagttttggtttaaccttgaacagcatgagcaagaccgatttcttaaagcttttgagttgctcgaagctagaaaaggaaagaccacgtttttatctcttgcctctggcatgtgtgacacaaatgatgaaaatccctctaagaatattcgatgcttgcttgtctctaacttgctcaagaaaatgggaaagattgcccttcaaatggaggctgtccagatgaaaattgtctttgattgtttcgaaaaaattttgtctgaaatgagtcgggaggattgcctgctttatgcttatgaaatgttgcagcccttgtataaagtttgcgaaggattttctgggactgtgatccctgaaaatatgaagcaattagcagaagaagtttctggacgtctacgcagtaaactaggcagccaaaattacgtacaagtttacaatgacatcaggaaacaactcaaggcaaaaagggataagaggaaacaggatgaaaagcgcttggctgttgttaatcctatgcagaatgccaagaggaaactgaggattgcagagaagcatcgggccaacaagaaaaggaagatgatgacaatgaaaatggggagatgggcacatcattccaagtcaaagtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DRIM

Protein Analysis

2626

Amino Acids

297.2

Weight (kDa)

7.59

Isoelectric Point (pI)

41.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UTP20_N PF07539 883 - 1494 8.8e-98 U3 small nucleolar RNA-associated protein 20, N-terminal
UTP20 PF20416 1699 - 1915 1e-65 U3 small nucleolar RNA-associated protein 20 domain
RYDR_ITPR PF01365 2128 - 2205 3.5e-06 RIH domain
UTP20_C PF23099 2517 - 2611 1.7e-11 Small subunit processome component 20 homolog, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1173, 5609, 7100
AarI CACCTGC 1 cut(s) 5849
AatII GACGTC 1 cut(s) 7648
Acc36I ACCTGC 2 cut(s) 5849, 6085
AccB1I GGYRCC 1 cut(s) 4440
AccB7I CCANNNNNTGG 1 cut(s) 6613
AccI GTMKAC 4 cut(s) 414, 989, 1587, 7648
AccIII TCCGGA 2 cut(s) 2979, 6334
AclI AACGTT 1 cut(s) 2440
AcoI YGGCCR 3 cut(s) 52, 2392, 3640
AcuI CTGAAG 9 cut(s) 1134, 1326, 2078, 2223, 2790, 3792, 4643, 6323, 7169
AcvI CACGTG 1 cut(s) 3302
AcyI GRCGYC 2 cut(s) 239, 7645
AdeI CACNNNGTG 1 cut(s) 7212
AfeI AGCGCT 1 cut(s) 7748
AflII CTTAAG 1 cut(s) 2699
AflIII ACRYGT 6 cut(s) 1807, 2653, 3262, 3301, 4413, 6993
AgeI ACCGGT 1 cut(s) 2253
AhlI ACTAGT 1 cut(s) 3886
AjiI CACGTC 1 cut(s) 3762
AjnI CCWGG 8 cut(s) 151, 378, 485, 1439, 3952, 4016, 4266, 5155
AjuI GAANNNNNNNTTGG 4 cut(s) 3264, 3296, 7065, 7097
AleI CACNNNNGTG 3 cut(s) 3709, 6513, 6994
AloI GAACNNNNNNTCC 2 cut(s) 3324, 3356
Alw21I GWGCWC 8 cut(s) 528, 3042, 3158, 4063, 4326, 6117, 6510, 7224
Alw44I GTGCAC 2 cut(s) 524, 7220
AlwNI CAGNNNCTG 5 cut(s) 830, 2557, 3770, 6421, 6760
Ama87I CYCGRG 1 cut(s) 369
Aor13HI TCCGGA 2 cut(s) 2979, 6334
Aor51HI AGCGCT 1 cut(s) 7748
AoxI GGCC 9 cut(s) 24, 52, 1545, 2179, 2392, 3640, 5737, 5848, 7812
ApaLI GTGCAC 2 cut(s) 524, 7220
ArsI GACNNNNNNTTYG 8 cut(s) 96, 128, 244, 276, 5630, 5662, 6266, 6298
AseI ATTAAT 1 cut(s) 596
AsiGI ACCGGT 1 cut(s) 2253
Asp700I GAANNNNTTC 6 cut(s) 2334, 3180, 3206, 3276, 6396, 6821
AspLEI GCGC 3 cut(s) 1402, 4581, 7749
AspS9I GGNCC 7 cut(s) 247, 1546, 1601, 2179, 3308, 6274, 7812
AsuC2I CCSGG 1 cut(s) 170
AsuHPI GGTGA 8 cut(s) 562, 1012, 2411, 3610, 4879, 5592, 5789, 6758
AsuII TTCGAA 3 cut(s) 291, 906, 7500
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 4 cut(s) 247, 1601, 3308, 6274
AxyI CCTNAGG 1 cut(s) 5845
BaeGI GKGCMC 4 cut(s) 528, 2949, 7224, 7861
BaeI ACNNNNGTAYC 6 cut(s) 646, 679, 3857, 3890, 5069, 5102
BalI TGGCCA 2 cut(s) 2394, 3642
BamHI GGATCC 2 cut(s) 1443, 5974
BanI GGYRCC 1 cut(s) 4440
BanII GRGCYC 3 cut(s) 1216, 3158, 4063
BauI CACGAG 1 cut(s) 227
BbrPI CACGTG 1 cut(s) 3302
BbsI GAAGAC 6 cut(s) 61, 106, 2490, 2954, 3849, 7157
Bbv12I GWGCWC 8 cut(s) 528, 3042, 3158, 4063, 4326, 6117, 6510, 7224
BbvCI CCTCAGC 1 cut(s) 5475
BceAI ACGGC 4 cut(s) 11, 226, 2499, 3495
BcgI CGANNNNNNTGC 2 cut(s) 312, 346
BciT130I CCWGG 8 cut(s) 153, 380, 487, 1441, 3954, 4018, 4268, 5157
BclI TGATCA 2 cut(s) 2383, 5707
BcnI CCSGG 1 cut(s) 170
BcuI ACTAGT 1 cut(s) 3886
BfoI RGCGCY 1 cut(s) 7750
BfrI CTTAAG 1 cut(s) 2699
BfuAI ACCTGC 2 cut(s) 5849, 6085
BglI GCCNNNNNGGC 1 cut(s) 1704
BglII AGATCT 4 cut(s) 373, 474, 3506, 5431
BlpI GCTNAGC 1 cut(s) 1145
BmcAI AGTACT 1 cut(s) 4119
Bme1390I CCNGG 9 cut(s) 153, 170, 380, 487, 1441, 3954, 4018, 4268, 5157
Bme18I GGWCC 4 cut(s) 247, 1601, 3308, 6274
BmeT110I CYCGRG 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 3762
BmgT120I GGNCC 7 cut(s) 247, 1546, 1601, 2179, 3308, 6274, 7812
BmrFI CCNGG 9 cut(s) 153, 170, 380, 487, 1441, 3954, 4018, 4268, 5157
BmrI ACTGGG 1 cut(s) 1867
BmuI ACTGGG 1 cut(s) 1867
BoxI GACNNNNGTC 1 cut(s) 973
BpiI GAAGAC 6 cut(s) 61, 106, 2490, 2954, 3849, 7157
BplI GAGNNNNNCTC 2 cut(s) 7043, 7075
BpmI CTGGAG 2 cut(s) 401, 5178
Bpu10I CCTNAGC 3 cut(s) 21, 5475, 7060
Bpu1102I GCTNAGC 1 cut(s) 1145
Bpu14I TTCGAA 3 cut(s) 291, 906, 7500
BpuEI CTTGAG 7 cut(s) 356, 2998, 4047, 4456, 6740, 7418, 7694
BpuMI CCSGG 1 cut(s) 170
Bsa29I ATCGAT 3 cut(s) 108, 1635, 6882
BsaAI YACGTR 2 cut(s) 3302, 7678
BsaBI GATNNNNATC 6 cut(s) 2240, 3423, 3511, 4668, 7257, 7389
BsaHI GRCGYC 2 cut(s) 239, 7645
BsaI GGTCTC 1 cut(s) 275
BsaJI CCNNGG 8 cut(s) 151, 404, 885, 2395, 3436, 3952, 3953, 4639
BsaWI WCCGGW 3 cut(s) 2253, 2979, 6334
BsaXI ACNNNNNCTCC 2 cut(s) 5267, 5297
Bse118I RCCGGY 2 cut(s) 50, 2253
Bse21I CCTNAGG 1 cut(s) 5845
Bse3DI GCAATG 7 cut(s) 254, 1214, 2360, 4457, 4552, 4737, 5853
Bse8I GATNNNNATC 6 cut(s) 2240, 3423, 3511, 4668, 7257, 7389
BseAI TCCGGA 2 cut(s) 2979, 6334
BseBI CCWGG 8 cut(s) 153, 380, 487, 1441, 3954, 4018, 4268, 5157
BseCI ATCGAT 3 cut(s) 108, 1635, 6882
BseDI CCNNGG 8 cut(s) 151, 404, 885, 2395, 3436, 3952, 3953, 4639
BseJI GATNNNNATC 6 cut(s) 2240, 3423, 3511, 4668, 7257, 7389
BseMI GCAATG 7 cut(s) 254, 1214, 2360, 4457, 4552, 4737, 5853
BseRI GAGGAG 2 cut(s) 2112, 6894
BseSI GKGCMC 4 cut(s) 528, 2949, 7224, 7861
BseX3I CGGCCG 1 cut(s) 52
BseYI CCCAGC 1 cut(s) 2972
BsgI GTGCAG 5 cut(s) 621, 1148, 5115, 6789, 7269
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 9 cut(s) 26, 54, 1547, 2181, 2394, 3642, 5739, 5850, 7814
BshNI GGYRCC 1 cut(s) 4440
BshTI ACCGGT 1 cut(s) 2253
BshVI ATCGAT 3 cut(s) 108, 1635, 6882
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 8 cut(s) 528, 3042, 3158, 4063, 4326, 6117, 6510, 7224
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 5 cut(s) 51, 169, 2254, 2980, 6335
BslFI GGGAC 8 cut(s) 19, 185, 352, 3294, 3716, 6287, 7248, 7609
BsmBI CGTCTC 2 cut(s) 85, 6683
BsmFI GGGAC 8 cut(s) 19, 185, 352, 3294, 3716, 6287, 7248, 7609
BsmI GAATGC 8 cut(s) 914, 1086, 1149, 4156, 4282, 4572, 7202, 7780
BsnI GGCC 9 cut(s) 26, 54, 1547, 2181, 2394, 3642, 5739, 5850, 7814
Bso31I GGTCTC 1 cut(s) 275
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 3 cut(s) 291, 906, 7500
Bsp13I TCCGGA 2 cut(s) 2979, 6334
Bsp1407I TGTACA 3 cut(s) 2691, 5731, 5953
Bsp1720I GCTNAGC 1 cut(s) 1145
BspANI GGCC 9 cut(s) 26, 54, 1547, 2181, 2394, 3642, 5739, 5850, 7814
BspDI ATCGAT 3 cut(s) 108, 1635, 6882
BspEI TCCGGA 2 cut(s) 2979, 6334
BspHI TCATGA 7 cut(s) 1320, 1531, 6810, 6895, 6907, 7069, 7119
BspMI ACCTGC 2 cut(s) 5849, 6085
BspQI GCTCTTC 1 cut(s) 3063
BspT104I TTCGAA 3 cut(s) 291, 906, 7500
BspT107I GGYRCC 1 cut(s) 4440
BspTI CTTAAG 1 cut(s) 2699
BspTNI GGTCTC 1 cut(s) 275
BsrDI GCAATG 7 cut(s) 254, 1214, 2360, 4457, 4552, 4737, 5853
BsrFI RCCGGY 2 cut(s) 50, 2253
BsrGI TGTACA 3 cut(s) 2691, 5731, 5953
BssAI RCCGGY 2 cut(s) 50, 2253
BssECI CCNNGG 8 cut(s) 151, 404, 885, 2395, 3436, 3952, 3953, 4639
BssNI GRCGYC 2 cut(s) 239, 7645
BssSI CACGAG 1 cut(s) 227
BssT1I CCWWGG 5 cut(s) 404, 885, 2395, 3436, 4639
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 8 cut(s) 153, 380, 487, 1441, 3954, 4018, 4268, 5157
Bst6I CTCTTC 7 cut(s) 16, 401, 3063, 3665, 3914, 4243, 4662
BstACI GRCGYC 2 cut(s) 239, 7645
BstAFI CTTAAG 1 cut(s) 2699
BstAUI TGTACA 3 cut(s) 2691, 5731, 5953
BstBAI YACGTR 2 cut(s) 3302, 7678
BstBI TTCGAA 3 cut(s) 291, 906, 7500
BstC8I GCNNGC 5 cut(s) 52, 1672, 6323, 7415, 7538
BstENI CCTNNNNNAGG 2 cut(s) 150, 760
BstH2I RGCGCY 1 cut(s) 7750
BstHHI GCGC 3 cut(s) 1402, 4581, 7749
BstMCI CGRYCG 1 cut(s) 55
BstNI CCWGG 8 cut(s) 153, 380, 487, 1441, 3954, 4018, 4268, 5157
BstNSI RCATGY 8 cut(s) 1811, 2999, 3266, 4417, 5052, 5351, 6997, 7372
BstPAI GACNNNNGTC 1 cut(s) 973
BstSCI CCNGG 9 cut(s) 151, 168, 378, 485, 1439, 3952, 4016, 4266, 5155
BstSLI GKGCMC 4 cut(s) 528, 2949, 7224, 7861
BstSNI TACGTA 1 cut(s) 7678
BstV2I GAAGAC 6 cut(s) 61, 106, 2490, 2954, 3849, 7157
BstXI CCANNNNNNTGG 1 cut(s) 6996
BstZI CGGCCG 1 cut(s) 52
Bsu15I ATCGAT 3 cut(s) 108, 1635, 6882
Bsu36I CCTNAGG 1 cut(s) 5845
BsuRI GGCC 9 cut(s) 26, 54, 1547, 2181, 2394, 3642, 5739, 5850, 7814
BsuTUI ATCGAT 3 cut(s) 108, 1635, 6882
BtgZI GCGATG 5 cut(s) 623, 1871, 2031, 3011, 5180
BtrI CACGTC 1 cut(s) 3762
BtsI GCAGTG 5 cut(s) 977, 3042, 3459, 3704, 6117
BveI ACCTGC 2 cut(s) 5849, 6085
Cac8I GCNNGC 5 cut(s) 52, 1672, 6323, 7415, 7538
CaiI CAGNNNCTG 5 cut(s) 830, 2557, 3770, 6421, 6760
CciI TCATGA 7 cut(s) 1320, 1531, 6810, 6895, 6907, 7069, 7119
CfoI GCGC 3 cut(s) 1402, 4581, 7749
Cfr10I RCCGGY 2 cut(s) 50, 2253
Cfr13I GGNCC 7 cut(s) 247, 1546, 1601, 2179, 3308, 6274, 7812
ClaI ATCGAT 3 cut(s) 108, 1635, 6882
CseI GACGC 3 cut(s) 67, 247, 2965
CspAI ACCGGT 1 cut(s) 2253
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
DraI TTTAAA 3 cut(s) 1491, 1926, 5328
DraIII CACNNNGTG 1 cut(s) 7212
EaeI YGGCCR 3 cut(s) 52, 2392, 3640
EagI CGGCCG 1 cut(s) 52
Eam1104I CTCTTC 7 cut(s) 16, 401, 3063, 3665, 3914, 4243, 4662
EarI CTCTTC 7 cut(s) 16, 401, 3063, 3665, 3914, 4243, 4662
EciI GGCGGA 1 cut(s) 37
Ecl136II GAGCTC 2 cut(s) 3156, 4061
EclXI CGGCCG 1 cut(s) 52
Eco105I TACGTA 1 cut(s) 7678
Eco130I CCWWGG 5 cut(s) 404, 885, 2395, 3436, 4639
Eco24I GRGCYC 3 cut(s) 1216, 3158, 4063
Eco31I GGTCTC 1 cut(s) 275
Eco32I GATATC 1 cut(s) 3474
Eco47I GGWCC 4 cut(s) 247, 1601, 3308, 6274
Eco47III AGCGCT 1 cut(s) 7748
Eco52I CGGCCG 1 cut(s) 52
Eco53kI GAGCTC 2 cut(s) 3156, 4061
Eco57I CTGAAG 9 cut(s) 1134, 1326, 2078, 2223, 2790, 3792, 4643, 6323, 7169
Eco72I CACGTG 1 cut(s) 3302
Eco81I CCTNAGG 1 cut(s) 5845
Eco88I CYCGRG 1 cut(s) 369
EcoICRI GAGCTC 2 cut(s) 3156, 4061
EcoNI CCTNNNNNAGG 2 cut(s) 150, 760
EcoO109I RGGNCCY 1 cut(s) 6274
EcoRI GAATTC 2 cut(s) 3572, 6007
EcoRII CCWGG 8 cut(s) 151, 378, 485, 1439, 3952, 4016, 4266, 5155
EcoRV GATATC 1 cut(s) 3474
EcoT14I CCWWGG 5 cut(s) 404, 885, 2395, 3436, 4639
EcoT22I ATGCAT 3 cut(s) 3298, 4009, 4384
EcoT38I GRGCYC 3 cut(s) 1216, 3158, 4063
ErhI CCWWGG 5 cut(s) 404, 885, 2395, 3436, 4639
Esp3I CGTCTC 2 cut(s) 85, 6683
FaqI GGGAC 8 cut(s) 19, 185, 352, 3294, 3716, 6287, 7248, 7609
FauI CCCGC 2 cut(s) 5399, 6189
FbaI TGATCA 2 cut(s) 2383, 5707
FblI GTMKAC 4 cut(s) 414, 989, 1587, 7648
FriOI GRGCYC 3 cut(s) 1216, 3158, 4063
GlaI GCGC 3 cut(s) 1401, 4580, 7748
GsaI CCCAGC 1 cut(s) 2976
GsuI CTGGAG 2 cut(s) 401, 5178
HaeII RGCGCY 1 cut(s) 7750
HaeIII GGCC 9 cut(s) 26, 54, 1547, 2181, 2394, 3642, 5739, 5850, 7814
HapII CCGG 5 cut(s) 51, 169, 2254, 2980, 6335
HgaI GACGC 3 cut(s) 67, 247, 2965
HhaI GCGC 3 cut(s) 1402, 4581, 7749
Hin1I GRCGYC 2 cut(s) 239, 7645
Hin6I GCGC 3 cut(s) 1400, 4579, 7747
HinP1I GCGC 3 cut(s) 1400, 4579, 7747
HincII GTYRAC 6 cut(s) 415, 1185, 1306, 2118, 5116, 6183
HindII GTYRAC 6 cut(s) 415, 1185, 1306, 2118, 5116, 6183
HindIII AAGCTT 7 cut(s) 530, 1240, 1405, 2205, 4961, 5591, 7308
HpaI GTTAAC 3 cut(s) 1306, 5116, 6183
HpaII CCGG 5 cut(s) 51, 169, 2254, 2980, 6335
HphI GGTGA 8 cut(s) 562, 1012, 2411, 3610, 4879, 5592, 5789, 6758
Hpy99I CGWCG 1 cut(s) 5817
Hsp92I GRCGYC 2 cut(s) 239, 7645
HspAI GCGC 3 cut(s) 1400, 4579, 7747
KflI GGGWCCC 1 cut(s) 6274
Kpn2I TCCGGA 2 cut(s) 2979, 6334
KroI GCCGGC 1 cut(s) 50
KroNI GCCGGC 1 cut(s) 52
Ksp22I TGATCA 2 cut(s) 2383, 5707
KspAI GTTAAC 3 cut(s) 1306, 5116, 6183
LguI GCTCTTC 1 cut(s) 3063
MfeI CAATTG 4 cut(s) 1262, 2840, 5016, 6309
MlsI TGGCCA 2 cut(s) 2394, 3642
MluNI TGGCCA 2 cut(s) 2394, 3642
MmeI TCCRAC 4 cut(s) 2439, 5265, 5723, 5952
Mox20I TGGCCA 2 cut(s) 2394, 3642
Mph1103I ATGCAT 3 cut(s) 3298, 4009, 4384
MroI TCCGGA 2 cut(s) 2979, 6334
MroNI GCCGGC 1 cut(s) 50
MroXI GAANNNNTTC 6 cut(s) 2334, 3180, 3206, 3276, 6396, 6821
MscI TGGCCA 2 cut(s) 2394, 3642
MslI CAYNNNNRTG 7 cut(s) 1091, 3709, 4865, 5047, 6043, 6513, 6994
Msp20I TGGCCA 2 cut(s) 2394, 3642
MspA1I CMGCKG 2 cut(s) 5406, 5681
MspCI CTTAAG 1 cut(s) 2699
MspI CCGG 5 cut(s) 51, 169, 2254, 2980, 6335
MspR9I CCNGG 9 cut(s) 153, 170, 380, 487, 1441, 3954, 4018, 4268, 5157
MunI CAATTG 4 cut(s) 1262, 2840, 5016, 6309
Mva1269I GAATGC 8 cut(s) 914, 1086, 1149, 4156, 4282, 4572, 7202, 7780
MvaI CCWGG 8 cut(s) 153, 380, 487, 1441, 3954, 4018, 4268, 5157
NaeI GCCGGC 1 cut(s) 52
NciI CCSGG 1 cut(s) 170
NgoMIV GCCGGC 1 cut(s) 50
NsiI ATGCAT 3 cut(s) 3298, 4009, 4384
NspI RCATGY 8 cut(s) 1811, 2999, 3266, 4417, 5052, 5351, 6997, 7372
NspV TTCGAA 3 cut(s) 291, 906, 7500
OliI CACNNNNGTG 3 cut(s) 3709, 6513, 6994
PagI TCATGA 7 cut(s) 1320, 1531, 6810, 6895, 6907, 7069, 7119
PaqCI CACCTGC 1 cut(s) 5849
PasI CCCWGGG 1 cut(s) 3953
PciI ACATGT 4 cut(s) 1807, 3262, 4413, 6993
PciSI GCTCTTC 1 cut(s) 3063
PcsI WCGNNNNNNNCGW 1 cut(s) 3555
PctI GAATGC 8 cut(s) 914, 1086, 1149, 4156, 4282, 4572, 7202, 7780
PdiI GCCGGC 1 cut(s) 52
PdmI GAANNNNTTC 6 cut(s) 2334, 3180, 3206, 3276, 6396, 6821
PflFI GACNNNGTC 1 cut(s) 5811
PflMI CCANNNNNTGG 1 cut(s) 6613
PfoI TCCNGGA 4 cut(s) 168, 378, 1439, 4266
PinAI ACCGGT 1 cut(s) 2253
PmaCI CACGTG 1 cut(s) 3302
PmlI CACGTG 1 cut(s) 3302
Ppu21I YACGTR 2 cut(s) 3302, 7678
PpuMI RGGWCCY 1 cut(s) 6274
PscI ACATGT 4 cut(s) 1807, 3262, 4413, 6993
PshAI GACNNNNGTC 1 cut(s) 973
PshBI ATTAAT 1 cut(s) 596
PsiI TTATAA 3 cut(s) 1173, 5609, 7100
Psp124BI GAGCTC 2 cut(s) 3158, 4063
Psp1406I AACGTT 1 cut(s) 2440
Psp5II RGGWCCY 1 cut(s) 6274
Psp6I CCWGG 8 cut(s) 151, 378, 485, 1439, 3952, 4016, 4266, 5155
PspCI CACGTG 1 cut(s) 3302
PspFI CCCAGC 1 cut(s) 2972
PspGI CCWGG 8 cut(s) 151, 378, 485, 1439, 3952, 4016, 4266, 5155
PspPI GGNCC 7 cut(s) 247, 1546, 1601, 2179, 3308, 6274, 7812
PspPPI RGGWCCY 1 cut(s) 6274
PstNI CAGNNNCTG 5 cut(s) 830, 2557, 3770, 6421, 6760
PsyI GACNNNGTC 1 cut(s) 5811
PvuII CAGCTG 1 cut(s) 5681
RseI CAYNNNNRTG 7 cut(s) 1091, 3709, 4865, 5047, 6043, 6513, 6994
SacI GAGCTC 2 cut(s) 3158, 4063
SalI GTCGAC 1 cut(s) 413
SapI GCTCTTC 1 cut(s) 3063
Sau96I GGNCC 7 cut(s) 247, 1546, 1601, 2179, 3308, 6274, 7812
ScaI AGTACT 1 cut(s) 4119
ScrFI CCNGG 9 cut(s) 153, 170, 380, 487, 1441, 3954, 4018, 4268, 5157
SfuI TTCGAA 3 cut(s) 291, 906, 7500
SinI GGWCC 4 cut(s) 247, 1601, 3308, 6274
SmiMI CAYNNNNRTG 7 cut(s) 1091, 3709, 4865, 5047, 6043, 6513, 6994
SmlI CTYRAG 8 cut(s) 335, 2699, 3013, 4062, 4435, 6719, 7433, 7709
SmoI CTYRAG 8 cut(s) 335, 2699, 3013, 4062, 4435, 6719, 7433, 7709
SnaBI TACGTA 1 cut(s) 7678
SpeI ACTAGT 1 cut(s) 3886
SspI AATATT 7 cut(s) 1666, 1989, 3223, 3984, 4144, 5961, 7405
SstI GAGCTC 2 cut(s) 3158, 4063
StyD4I CCNGG 9 cut(s) 151, 168, 378, 485, 1439, 3952, 4016, 4266, 5155
StyI CCWWGG 5 cut(s) 404, 885, 2395, 3436, 4639
TaqII GACCGA 1 cut(s) 7311
TatI WGTACW 9 cut(s) 952, 2474, 2691, 2773, 4117, 5731, 5953, 6187, 6723
TauI GCSGC 6 cut(s) 57, 60, 63, 4975, 5853, 6668
TspGWI ACGGA 4 cut(s) 2948, 4307, 5225, 7033
Tth111I GACNNNGTC 1 cut(s) 5811
Van91I CCANNNNNTGG 1 cut(s) 6613
Vha464I CTTAAG 1 cut(s) 2699
VneI GTGCAC 2 cut(s) 524, 7220
VpaK11BI GGWCC 4 cut(s) 247, 1601, 3308, 6274
VspI ATTAAT 1 cut(s) 596
XagI CCTNNNNNAGG 2 cut(s) 150, 760
XbaI TCTAGA 5 cut(s) 305, 773, 2165, 3683, 4183
XceI RCATGY 8 cut(s) 1811, 2999, 3266, 4417, 5052, 5351, 6997, 7372
XcmI CCANNNNNNNNNTGG 1 cut(s) 4249
XmiI GTMKAC 4 cut(s) 414, 989, 1587, 7648
XmnI GAANNNNTTC 6 cut(s) 2334, 3180, 3206, 3276, 6396, 6821
ZraI GACGTC 1 cut(s) 7646
ZrmI AGTACT 1 cut(s) 4119
Zsp2I ATGCAT 3 cut(s) 3298, 4009, 4384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.