Rmu_sc0000953.1_g000002

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000953.1
OG0012435
Physical Location & Seq
Reverse (-)
2624 .. 5118
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 828 bp
atgacgctgggaggagccacgacgacgacgacgcagcagcaagcctacggcgagccatggtactgggacaaccgctacgccaacgaatccggggcgttcgattggtaccagaagtaccaatctctagctcccattatcaatctgtacgtcccccgccaccgcatcctcgtcgtcggctgcggcaactccgcatttagcgaaggaatggcggacgatgggtacgaggaggtggtcagtatcgacatttcatctgtggtcatccaagctatgcaggagaagcactccaatcgtcaagaccttaaatatttggaaatggatgtgagagatatgaatgctttccaagccgcttcctttgatgctgttattgacaaaggaactctagactctcttctggtaagttatgctaatctatgtagtgcattactgtttgtattatttttagcaaggctgtttttaatatgcaaagtttttgctcagtgcggaagtaattcacaacaaaatgctgctcggatgctcagtgaagtttggagggtcctcaaggataaaggagtctatattctggtcacatatggagccccgttgtatcgtttacggttgctgaaagaatcaagctcgtgggttataaaactccatgtgatagataaacttgtttccgatgataaatcagaacatccaatatgggagctgacaaatcctgttcccttgaatgatgatgggagctcagcagaggaattgctaggaaacaaccctgatgttcattttatttacatttgtacgaaggataactctttgaagccaggccttaagcgtgaagtgtcagtcgactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

275

Amino Acids

30.95

Weight (kDa)

5.08

Isoelectric Point (pI)

24.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPMT PF05724 21 - 105 7.2e-06 Thiopurine S-methyltransferase (TPMT)
Methyltransf_31 PF13847 54 - 191 9.8e-13 Methyltransferase domain
Methyltransf_25 PF13649 55 - 125 1.3e-09 Methyltransferase domain
Methyltransf_11 PF08241 56 - 128 2.7e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012435)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G17365 AT3G17365
fragaria_vesca FvH4_4g21180 FvH4_4g21180
malus_domestica MD13G1144200.v1.1
prunus_persica Prupe.1G108600_v2.0.a1 Prupe.1G108600_v2.0.a1
pyrus_communis pycom16g12340
rosa_chinensis RchiOBHm_Chr4g0426751
rosa_laevigata RLG00000007280
rosa_multiflora Rmu_co8157368.1_g000001 Rmu_sc0000953.1_g000002
rosa_roxburghii Rroxscaffold_5G00368750
rosa_rugosa Rorug04G0214600
rosa_samantha Rh4AG269700 Rh4BG276000 Rh4CG290600 Rh4DG273000
rosa_wichuraiana Rw4G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 623
Acc65I GGTACC 1 cut(s) 105
AccB1I GGYRCC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 822
AciI CCGC 8 cut(s) 73, 154, 160, 180, 189, 209, 345, 480
AfaI GTAC 6 cut(s) 62, 107, 116, 146, 221, 775
AflII CTTAAG 1 cut(s) 803
AgsI TTSAA 2 cut(s) 706, 793
AjnI CCWGG 1 cut(s) 796
AluBI AGCT 5 cut(s) 128, 266, 612, 685, 720
AluI AGCT 5 cut(s) 128, 266, 612, 685, 720
Alw21I GWGCWC 1 cut(s) 722
AoxI GGCC 1 cut(s) 799
ApeKI GCWGC 4 cut(s) 34, 37, 177, 503
Asp700I GAANNNNTTC 2 cut(s) 335, 487
Asp718I GGTACC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 532
AsuC2I CCSGG 1 cut(s) 91
AvaII GGWCC 1 cut(s) 532
BanI GGYRCC 1 cut(s) 105
BanII GRGCYC 2 cut(s) 577, 722
BauI CACGAG 1 cut(s) 613
Bbv12I GWGCWC 1 cut(s) 722
BbvI GCAGC 4 cut(s) 46, 49, 164, 490
BccI CCATC 2 cut(s) 209, 707
BceAI ACGGC 1 cut(s) 64
BcgI CGANNNNNNTGC 4 cut(s) 151, 185, 269, 303
BciT130I CCWGG 1 cut(s) 798
BcnI CCSGG 1 cut(s) 91
BfaI CTAG 3 cut(s) 125, 380, 737
BfrI CTTAAG 1 cut(s) 803
BisI GCNGC 6 cut(s) 35, 38, 178, 181, 345, 504
BlpI GCTNAGC 1 cut(s) 721
BlsI GCNGC 6 cut(s) 36, 39, 179, 182, 346, 505
Bme1390I CCNGG 2 cut(s) 91, 798
Bme18I GGWCC 1 cut(s) 532
BmgT120I GGNCC 1 cut(s) 532
BmiI GGNNCC 4 cut(s) 16, 107, 533, 574
BmrFI CCNGG 2 cut(s) 91, 798
BmrI ACTGGG 1 cut(s) 73
BmsI GCATC 3 cut(s) 171, 346, 501
BmuI ACTGGG 1 cut(s) 73
BplI GAGNNNNNCTC 2 cut(s) 266, 298
Bpu1102I GCTNAGC 1 cut(s) 721
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 91
BsaJI CCNNGG 2 cut(s) 56, 90
Bse1I ACTGG 1 cut(s) 68
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 2 cut(s) 56, 90
BseGI GGATG 5 cut(s) 162, 258, 322, 516, 670
BseMII CTCAG 3 cut(s) 488, 529, 735
BseNI ACTGG 1 cut(s) 68
BseRI GAGGAG 2 cut(s) 27, 239
BseXI GCAGC 4 cut(s) 46, 49, 164, 490
BseYI CCCAGC 1 cut(s) 7
BshFI GGCC 1 cut(s) 801
BshNI GGYRCC 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 722
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 80, 134
BsmFI GGGAC 2 cut(s) 80, 134
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 1 cut(s) 801
Bsp1286I GDGCHC 2 cut(s) 577, 722
Bsp1720I GCTNAGC 1 cut(s) 721
Bsp19I CCATGG 1 cut(s) 56
BspACI CCGC 8 cut(s) 73, 154, 160, 180, 189, 209, 345, 480
BspANI GGCC 1 cut(s) 801
BspCNI CTCAG 3 cut(s) 487, 528, 734
BspLI GGNNCC 4 cut(s) 16, 107, 533, 574
BspT107I GGYRCC 1 cut(s) 105
BspTI CTTAAG 1 cut(s) 803
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 2 cut(s) 56, 90
BssSI CACGAG 1 cut(s) 613
BssT1I CCWWGG 1 cut(s) 56
Bst2BI CACGAG 1 cut(s) 613
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 2 cut(s) 426, 594
Bst6I CTCTTC 1 cut(s) 393
BstAFI CTTAAG 1 cut(s) 803
BstC8I GCNNGC 2 cut(s) 42, 53
BstDEI CTNAG 3 cut(s) 474, 515, 721
BstDSI CCRYGG 1 cut(s) 56
BstF5I GGATG 5 cut(s) 162, 258, 322, 516, 670
BstMWI GCNNNNNNNGC 2 cut(s) 277, 341
BstNI CCWGG 1 cut(s) 798
BstSCI CCNGG 2 cut(s) 89, 796
BstV1I GCAGC 4 cut(s) 46, 49, 164, 490
BstXI CCANNNNNNTGG 1 cut(s) 63
BsuRI GGCC 1 cut(s) 801
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 5 cut(s) 162, 258, 322, 516, 670
BtsIMutI CAGTG 2 cut(s) 482, 523
Cac8I GCNNGC 2 cut(s) 42, 53
Cfr13I GGNCC 1 cut(s) 532
CseI GACGC 2 cut(s) 13, 40
Csp6I GTAC 6 cut(s) 61, 106, 115, 145, 220, 774
CviAII CATG 2 cut(s) 57, 632
CviQI GTAC 6 cut(s) 61, 106, 115, 145, 220, 774
DdeI CTNAG 3 cut(s) 474, 515, 721
Eam1104I CTCTTC 1 cut(s) 393
EarI CTCTTC 1 cut(s) 393
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 1 cut(s) 720
Eco130I CCWWGG 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 801
Eco24I GRGCYC 2 cut(s) 577, 722
Eco47I GGWCC 1 cut(s) 532
Eco53kI GAGCTC 1 cut(s) 720
EcoICRI GAGCTC 1 cut(s) 720
EcoO109I RGGNCCY 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 56
EcoT38I GRGCYC 2 cut(s) 577, 722
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 2 cut(s) 60, 635
FaqI GGGAC 2 cut(s) 80, 134
FatI CATG 2 cut(s) 56, 631
FauI CCCGC 1 cut(s) 161
FauNDI CATATG 1 cut(s) 568
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 6 cut(s) 35, 38, 178, 181, 345, 504
FokI GGATG 5 cut(s) 149, 245, 329, 523, 657
FriOI GRGCYC 2 cut(s) 577, 722
Fsp4HI GCNGC 6 cut(s) 35, 38, 178, 181, 345, 504
FspBI CTAG 3 cut(s) 125, 380, 737
GluI GCNGC 6 cut(s) 35, 38, 178, 181, 345, 504
GsaI CCCAGC 1 cut(s) 11
HaeIII GGCC 1 cut(s) 801
HapII CCGG 1 cut(s) 90
HgaI GACGC 2 cut(s) 13, 40
Hin1II CATG 2 cut(s) 60, 635
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 4 cut(s) 86, 383, 549, 605
HpaII CCGG 1 cut(s) 90
Hpy166II GTNNAC 2 cut(s) 590, 823
Hpy188I TCNGA 3 cut(s) 510, 655, 667
Hpy188III TCNNGA 2 cut(s) 293, 380
Hpy8I GTNNAC 2 cut(s) 590, 823
Hpy99I CGWCG 6 cut(s) 25, 28, 31, 34, 173, 176
HpyAV CCTTC 2 cut(s) 194, 772
HpyCH4III ACNGT 2 cut(s) 426, 594
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 3 cut(s) 271, 419, 462
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 341
HpyF3I CTNAG 3 cut(s) 474, 515, 721
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 2 cut(s) 60, 635
KpnI GGTACC 1 cut(s) 109
LmnI GCTCC 5 cut(s) 14, 133, 572, 682, 717
Lsp1109I GCAGC 4 cut(s) 46, 49, 164, 490
LweI GCATC 3 cut(s) 171, 346, 501
MaeI CTAG 3 cut(s) 125, 380, 737
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 562
MboII GAAGA 1 cut(s) 380
MhlI GDGCHC 2 cut(s) 577, 722
MluCI AATT 2 cut(s) 487, 731
MlyI GAGTC 2 cut(s) 377, 558
MnlI CCTC 7 cut(s) 5, 176, 217, 220, 522, 545, 721
MroXI GAANNNNTTC 2 cut(s) 335, 487
MseI TTAA 3 cut(s) 300, 455, 804
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 2 cut(s) 91, 798
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 798
MwoI GCNNNNNNNGC 2 cut(s) 277, 341
NciI CCSGG 1 cut(s) 91
NcoI CCATGG 1 cut(s) 56
NdeI CATATG 1 cut(s) 568
NlaIII CATG 2 cut(s) 60, 635
NlaIV GGNNCC 4 cut(s) 16, 107, 533, 574
NmuCI GTSAC 1 cut(s) 562
PceI AGGCCT 1 cut(s) 801
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 219
PctI GAATGC 1 cut(s) 337
PdmI GAANNNNTTC 2 cut(s) 335, 487
PfeI GAWTC 2 cut(s) 86, 605
PkrI GCNGC 6 cut(s) 36, 39, 179, 182, 346, 505
PleI GAGTC 2 cut(s) 377, 557
PpsI GAGTC 2 cut(s) 377, 557
PpuMI RGGWCCY 1 cut(s) 532
PsiI TTATAA 1 cut(s) 623
Psp124BI GAGCTC 1 cut(s) 722
Psp5II RGGWCCY 1 cut(s) 532
Psp6I CCWGG 1 cut(s) 796
PspFI CCCAGC 1 cut(s) 7
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 4 cut(s) 16, 107, 533, 574
PspPI GGNCC 1 cut(s) 532
PspPPI RGGWCCY 1 cut(s) 532
RsaI GTAC 6 cut(s) 62, 107, 116, 146, 221, 775
RsaNI GTAC 6 cut(s) 61, 106, 115, 145, 220, 774
SacI GAGCTC 1 cut(s) 722
SalI GTCGAC 1 cut(s) 821
SaqAI TTAA 3 cut(s) 300, 455, 804
SatI GCNGC 6 cut(s) 35, 38, 178, 181, 345, 504
Sau96I GGNCC 1 cut(s) 532
SchI GAGTC 2 cut(s) 377, 558
ScrFI CCNGG 2 cut(s) 91, 798
SduI GDGCHC 2 cut(s) 577, 722
SetI ASST 8 cut(s) 130, 150, 231, 268, 300, 614, 687, 722
SfaNI GCATC 3 cut(s) 171, 346, 501
SinI GGWCC 1 cut(s) 532
SmlI CTYRAG 2 cut(s) 536, 803
SmoI CTYRAG 2 cut(s) 536, 803
Sse9I AATT 2 cut(s) 487, 731
SseBI AGGCCT 1 cut(s) 801
SsiI CCGC 8 cut(s) 73, 154, 160, 180, 189, 209, 345, 480
SspI AATATT 1 cut(s) 305
SspMI CTAG 3 cut(s) 125, 380, 737
SstI GAGCTC 1 cut(s) 722
StuI AGGCCT 1 cut(s) 801
StyD4I CCNGG 2 cut(s) 89, 796
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 426, 594
TaiI ACGT 1 cut(s) 150
TaqI TCGA 3 cut(s) 99, 240, 822
TasI AATT 2 cut(s) 487, 731
TauI GCSGC 2 cut(s) 183, 347
TfiI GAWTC 2 cut(s) 86, 605
Tru1I TTAA 3 cut(s) 300, 455, 804
Tru9I TTAA 3 cut(s) 300, 455, 804
TscAI CASTG 2 cut(s) 482, 523
TseFI GTSAC 1 cut(s) 562
TseI GCWGC 4 cut(s) 34, 37, 177, 503
Tsp45I GTSAC 1 cut(s) 562
TspDTI ATGAA 3 cut(s) 237, 344, 746
TspRI CASTG 2 cut(s) 482, 523
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 532
XbaI TCTAGA 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 822
XmnI GAANNNNTTC 2 cut(s) 335, 487
XspI CTAG 3 cut(s) 125, 380, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.