Rmu_sc0001170.1_g000006

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001170.1
OG0013592
Physical Location & Seq
Forward (+)
15235 .. 16356
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1122 bp
atgctactattggctggccaagtccattctctacgccaacttggtgattacagaacacaacatctagcaaaacaacaaaattacatcccatatagattgagtgagagcgagttagcttacgggtcgggtccgaaacgagctaggaattccatgggctaccggatagtgcccaagcttttactgtacgtcgattccacacgtgccattttctcatacgtgctaaaggctgaaggggacccacttaaattttccctctttcaaatcctcgtcgctttccttccgccactatatatacctcgcccctccaaaccaaaattcaaatctcacagacgcggtaccctaaccaccatgttatcgtcggcgattgtgatccccgtcctcttcatcctagccgtcgttgctttgctcctctcctactacctccgccgcagccgccgcctccgatcacaacctctccgtcaaccagacgccgccgcgcctgtccagagctcacactctcgccgccggcaagtctctcctgctccttcctccgccggcctcctcctcccgcgcttcaaattctccgacctgaaacgccccggctcagtcaccgtcggcggagactgcgcgatttgcctggcggagttcgagccggaccacgtgctccgcctcctccctctctgcctccacgccttccacgaggattgcatcgacacctggctcgccggcgagtggttgatttgtccggtttgccgcgccgaggtcttccggtccgactcggacctcctccagacagcctcgtccggcggaagcttccggctggcgatcggcagcgtcagccgcggactgcggcaagacacggcggccaattcgagctcgcaaactcactcaattcgcggaggtgaattggagtatgttgtggatgaggagtgggagcttcaactcagagatgcggttcttcctgttcacgacgaaggcgaggataacgagatcgtcgccgtcgttaccgacagccaacctccgccgtctacggcgccaactttggagtatcagagctcctgcggcatgattttcacagggagcagccgtcgttggacggagttatccgagggactggaaatgattcaccggtggctatcctga

Protein Analysis

373

Amino Acids

41.63

Weight (kDa)

8.8

Isoelectric Point (pI)

62.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 201 - 245 9.1e-11 Ring finger domain
zf-RING_11 PF17123 201 - 230 3.1e-09 RING-like zinc finger
zf-C3HC4 PF00097 202 - 244 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 202 - 244 9.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 778
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 2 cut(s) 335, 1012
AccI GTMKAC 1 cut(s) 1007
AccII CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
AclWI GGATC 1 cut(s) 364
AcoI YGGCCR 2 cut(s) 16, 843
AcsI RAATTY 4 cut(s) 145, 245, 314, 557
AcuI CTGAAG 1 cut(s) 249
AcvI CACGTG 2 cut(s) 200, 640
AcyI GRCGYC 2 cut(s) 468, 1013
AfaI GTAC 2 cut(s) 185, 337
AfiI CCNNNNNNNGG 2 cut(s) 711, 739
AflIII ACRYGT 1 cut(s) 197
AgeI ACCGGT 1 cut(s) 1107
AgsI TTSAA 4 cut(s) 260, 319, 556, 920
AjnI CCWGG 2 cut(s) 615, 695
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AluBI AGCT 8 cut(s) 116, 140, 175, 489, 792, 855, 916, 1035
AluI AGCT 8 cut(s) 116, 140, 175, 489, 792, 855, 916, 1035
Alw21I GWGCWC 4 cut(s) 491, 645, 857, 1037
Alw26I GTCTC 2 cut(s) 517, 594
AlwI GGATC 1 cut(s) 364
AoxI GGCC 3 cut(s) 16, 535, 843
ApeKI GCWGC 3 cut(s) 429, 810, 1062
ApoI RAATTY 4 cut(s) 145, 245, 314, 557
AsiGI ACCGGT 1 cut(s) 1107
Asp700I GAANNNNTTC 1 cut(s) 1101
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 5 cut(s) 478, 552, 608, 737, 1015
AspS9I GGNCC 5 cut(s) 128, 235, 634, 750, 760
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 4 cut(s) 56, 580, 893, 1097
AvaII GGWCC 5 cut(s) 128, 235, 634, 750, 760
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 18
BanI GGYRCC 2 cut(s) 335, 1012
BanII GRGCYC 3 cut(s) 491, 857, 1037
BauI CACGAG 1 cut(s) 677
BbrPI CACGTG 2 cut(s) 200, 640
BbsI GAAGAC 1 cut(s) 736
Bbv12I GWGCWC 4 cut(s) 491, 645, 857, 1037
BbvI GCAGC 3 cut(s) 441, 822, 1074
BceAI ACGGC 6 cut(s) 377, 855, 962, 988, 1026, 1050
BciT130I CCWGG 2 cut(s) 617, 697
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 2 cut(s) 517, 594
BfaI CTAG 3 cut(s) 65, 141, 389
BfoI RGCGCY 1 cut(s) 1016
Bme1390I CCNGG 3 cut(s) 579, 617, 697
Bme18I GGWCC 5 cut(s) 128, 235, 634, 750, 760
BmgT120I GGNCC 5 cut(s) 128, 235, 634, 750, 760
BmiI GGNNCC 5 cut(s) 129, 236, 237, 337, 1014
BmrFI CCNGG 3 cut(s) 579, 617, 697
BmsI GCATC 2 cut(s) 696, 919
BpiI GAAGAC 1 cut(s) 736
BpmI CTGGAG 1 cut(s) 752
BpuMI CCSGG 1 cut(s) 579
BsaAI YACGTR 3 cut(s) 200, 217, 640
BsaBI GATNNNNATC 1 cut(s) 368
BsaHI GRCGYC 2 cut(s) 468, 1013
BsaJI CCNNGG 5 cut(s) 150, 577, 738, 820, 1086
BsaWI WCCGGW 4 cut(s) 159, 724, 747, 1107
BsaXI ACNNNNNCTCC 4 cut(s) 438, 468, 1051, 1081
Bsc4I CCNNNNNNNGG 2 cut(s) 711, 739
Bse118I RCCGGY 4 cut(s) 504, 533, 704, 1107
Bse1I ACTGG 1 cut(s) 1098
Bse8I GATNNNNATC 1 cut(s) 368
BseBI CCWGG 2 cut(s) 617, 697
BseDI CCNNGG 5 cut(s) 150, 577, 738, 820, 1086
BseGI GGATG 3 cut(s) 84, 384, 907
BseJI GATNNNNATC 1 cut(s) 368
BseLI CCNNNNNNNGG 2 cut(s) 711, 739
BseMII CTCAG 2 cut(s) 597, 937
BseNI ACTGG 1 cut(s) 1098
BseRI GAGGAG 6 cut(s) 398, 530, 533, 641, 755, 920
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 3 cut(s) 441, 822, 1074
Bsh1236I CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
Bsh1285I CGRYCG 1 cut(s) 807
BshFI GGCC 3 cut(s) 18, 537, 845
BshNI GGYRCC 2 cut(s) 335, 1012
BshTI ACCGGT 1 cut(s) 1107
BsiEI CGRYCG 1 cut(s) 807
BsiHKAI GWGCWC 4 cut(s) 491, 645, 857, 1037
BslFI GGGAC 2 cut(s) 248, 1104
BslI CCNNNNNNNGG 2 cut(s) 711, 739
BsmAI GTCTC 2 cut(s) 517, 594
BsmFI GGGAC 2 cut(s) 248, 1104
BsnI GGCC 3 cut(s) 18, 537, 845
Bsp1286I GDGCHC 5 cut(s) 171, 491, 645, 857, 1037
Bsp143I GATC 4 cut(s) 369, 443, 804, 969
Bsp19I CCATGG 1 cut(s) 150
BspANI GGCC 3 cut(s) 18, 537, 845
BspCNI CTCAG 2 cut(s) 596, 936
BspFNI CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
BspLI GGNNCC 5 cut(s) 129, 236, 237, 337, 1014
BspPI GGATC 1 cut(s) 364
BspT107I GGYRCC 2 cut(s) 335, 1012
BsrFI RCCGGY 4 cut(s) 504, 533, 704, 1107
BsrI ACTGG 1 cut(s) 1098
BssAI RCCGGY 4 cut(s) 504, 533, 704, 1107
BssECI CCNNGG 5 cut(s) 150, 577, 738, 820, 1086
BssMI GATC 4 cut(s) 369, 443, 804, 969
BssNI GRCGYC 2 cut(s) 468, 1013
BssSI CACGAG 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 150
Bst2BI CACGAG 1 cut(s) 677
Bst2UI CCWGG 2 cut(s) 617, 697
Bst4CI ACNGT 2 cut(s) 183, 592
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 2 cut(s) 468, 1013
BstBAI YACGTR 3 cut(s) 200, 217, 640
BstC8I GCNNGC 7 cut(s) 16, 506, 535, 702, 706, 801, 857
BstDEI CTNAG 2 cut(s) 583, 923
BstDSI CCRYGG 2 cut(s) 150, 820
BstF5I GGATG 3 cut(s) 84, 384, 907
BstFNI CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
BstH2I RGCGCY 1 cut(s) 1016
BstHHI GCGC 5 cut(s) 478, 552, 608, 737, 1015
BstKTI GATC 4 cut(s) 372, 446, 807, 972
BstMAI GTCTC 2 cut(s) 517, 594
BstMBI GATC 4 cut(s) 369, 443, 804, 969
BstMCI CGRYCG 1 cut(s) 807
BstMWI GCNNNNNNNGC 8 cut(s) 398, 432, 435, 603, 612, 816, 819, 1041
BstNI CCWGG 2 cut(s) 617, 697
BstSCI CCNGG 3 cut(s) 577, 615, 695
BstSLI GKGCMC 1 cut(s) 171
BstUI CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
BstV1I GCAGC 3 cut(s) 441, 822, 1074
BstV2I GAAGAC 1 cut(s) 736
BsuRI GGCC 3 cut(s) 18, 537, 845
BtgI CCRYGG 2 cut(s) 150, 820
BtsCI GGATG 3 cut(s) 84, 384, 907
Cac8I GCNNGC 7 cut(s) 16, 506, 535, 702, 706, 801, 857
CfoI GCGC 5 cut(s) 478, 552, 608, 737, 1015
Cfr10I RCCGGY 4 cut(s) 504, 533, 704, 1107
Cfr13I GGNCC 5 cut(s) 128, 235, 634, 750, 760
Cfr42I CCGCGG 1 cut(s) 823
CpoI CGGWCCG 1 cut(s) 750
CseI GACGC 3 cut(s) 339, 476, 802
Csp6I GTAC 2 cut(s) 184, 336
CspAI ACCGGT 1 cut(s) 1107
CspCI CAANNNNNGTGG 2 cut(s) 665, 700
CspI CGGWCCG 1 cut(s) 750
CviAII CATG 3 cut(s) 151, 349, 1045
CviQI GTAC 2 cut(s) 184, 336
DdeI CTNAG 2 cut(s) 583, 923
DinI GGCGCC 1 cut(s) 1014
DpnI GATC 4 cut(s) 371, 445, 806, 971
DpnII GATC 4 cut(s) 369, 443, 804, 969
DrdI GACNNNNNNGTC 1 cut(s) 778
DseDI GACNNNNNNGTC 1 cut(s) 778
EaeI YGGCCR 2 cut(s) 16, 843
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
EciI GGCGGA 8 cut(s) 270, 413, 520, 612, 635, 635, 801, 990
Ecl136II GAGCTC 3 cut(s) 489, 855, 1035
Eco130I CCWWGG 1 cut(s) 150
Eco24I GRGCYC 3 cut(s) 491, 857, 1037
Eco47I GGWCC 5 cut(s) 128, 235, 634, 750, 760
Eco53kI GAGCTC 3 cut(s) 489, 855, 1035
Eco57I CTGAAG 1 cut(s) 249
Eco72I CACGTG 2 cut(s) 200, 640
EcoICRI GAGCTC 3 cut(s) 489, 855, 1035
EcoO109I RGGNCCY 1 cut(s) 235
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 2 cut(s) 615, 695
EcoT14I CCWWGG 1 cut(s) 150
EcoT38I GRGCYC 3 cut(s) 491, 857, 1037
EgeI GGCGCC 1 cut(s) 1014
EheI GGCGCC 1 cut(s) 1014
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 154, 352, 1048
FaqI GGGAC 2 cut(s) 248, 1104
FatI CATG 3 cut(s) 150, 348, 1044
FauI CCCGC 1 cut(s) 555
FblI GTMKAC 1 cut(s) 1007
FokI GGATG 3 cut(s) 71, 371, 914
FriOI GRGCYC 3 cut(s) 491, 857, 1037
FspBI CTAG 3 cut(s) 65, 141, 389
GlaI GCGC 5 cut(s) 477, 551, 607, 736, 1014
GsuI CTGGAG 1 cut(s) 752
HaeII RGCGCY 1 cut(s) 1016
HaeIII GGCC 3 cut(s) 18, 537, 845
HgaI GACGC 3 cut(s) 339, 476, 802
HhaI GCGC 5 cut(s) 478, 552, 608, 737, 1015
Hin1I GRCGYC 2 cut(s) 468, 1013
Hin1II CATG 3 cut(s) 154, 352, 1048
Hin6I GCGC 5 cut(s) 476, 550, 606, 735, 1013
HinP1I GCGC 5 cut(s) 476, 550, 606, 735, 1013
HincII GTYRAC 1 cut(s) 461
HindII GTYRAC 1 cut(s) 461
HindIII AAGCTT 2 cut(s) 173, 790
HinfI GANTC 3 cut(s) 191, 755, 1102
HphI GGTGA 4 cut(s) 56, 580, 893, 1097
Hpy166II GTNNAC 3 cut(s) 461, 946, 1008
Hpy188I TCNGA 8 cut(s) 132, 443, 565, 754, 760, 926, 1032, 1087
Hpy188III TCNNGA 4 cut(s) 484, 769, 947, 1119
Hpy8I GTNNAC 3 cut(s) 461, 946, 1008
Hpy99I CGWCG 9 cut(s) 191, 272, 361, 398, 596, 953, 977, 983, 1071
HpyAV CCTTC 5 cut(s) 224, 287, 534, 682, 947
HpyCH4III ACNGT 2 cut(s) 183, 592
HpyCH4IV ACGT 4 cut(s) 186, 199, 216, 639
HpyCH4V TGCA 1 cut(s) 687
HpyF10VI GCNNNNNNNGC 8 cut(s) 398, 432, 435, 603, 612, 816, 819, 1041
HpyF3I CTNAG 2 cut(s) 583, 923
HpySE526I ACGT 4 cut(s) 186, 199, 216, 639
Hsp92I GRCGYC 2 cut(s) 468, 1013
Hsp92II CATG 3 cut(s) 154, 352, 1048
HspAI GCGC 5 cut(s) 476, 550, 606, 735, 1013
KasI GGCGCC 1 cut(s) 1012
KflI GGGWCCC 1 cut(s) 235
KpnI GGTACC 1 cut(s) 339
KroI GCCGGC 3 cut(s) 504, 533, 704
KroNI GCCGGC 3 cut(s) 506, 535, 706
KspI CCGCGG 1 cut(s) 823
Kzo9I GATC 4 cut(s) 369, 443, 804, 969
LmnI GCTCC 6 cut(s) 411, 526, 648, 913, 1040, 1059
Lsp1109I GCAGC 3 cut(s) 441, 822, 1074
LweI GCATC 2 cut(s) 696, 919
MaeI CTAG 3 cut(s) 65, 141, 389
MaeII ACGT 4 cut(s) 186, 199, 216, 639
MaeIII GTNAC 2 cut(s) 586, 982
MalI GATC 4 cut(s) 371, 445, 806, 971
MboI GATC 4 cut(s) 369, 443, 804, 969
MboII GAAGA 3 cut(s) 373, 736, 929
MhlI GDGCHC 5 cut(s) 171, 491, 645, 857, 1037
MlsI TGGCCA 1 cut(s) 18
MluCI AATT 8 cut(s) 79, 145, 245, 314, 557, 847, 870, 884
MluNI TGGCCA 1 cut(s) 18
Mly113I GGCGCC 1 cut(s) 1013
MlyI GAGTC 1 cut(s) 749
MmeI TCCRAC 3 cut(s) 588, 777, 1052
Mox20I TGGCCA 1 cut(s) 18
MreI CGCCGGCG 1 cut(s) 704
MroNI GCCGGC 3 cut(s) 504, 533, 704
MroXI GAANNNNTTC 1 cut(s) 1101
MscI TGGCCA 1 cut(s) 18
MseI TTAA 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 822
MspR9I CCNGG 3 cut(s) 579, 617, 697
MvaI CCWGG 2 cut(s) 617, 697
MvnI CGCG 7 cut(s) 333, 476, 550, 608, 735, 822, 876
MwoI GCNNNNNNNGC 8 cut(s) 398, 432, 435, 603, 612, 816, 819, 1041
NaeI GCCGGC 3 cut(s) 506, 535, 706
NarI GGCGCC 1 cut(s) 1013
NciI CCSGG 1 cut(s) 579
NcoI CCATGG 1 cut(s) 150
NdeII GATC 4 cut(s) 369, 443, 804, 969
NgoMIV GCCGGC 3 cut(s) 504, 533, 704
NlaIII CATG 3 cut(s) 154, 352, 1048
NlaIV GGNNCC 5 cut(s) 129, 236, 237, 337, 1014
NmeAIII GCCGAG 1 cut(s) 763
NmuCI GTSAC 1 cut(s) 586
PcsI WCGNNNNNNNCGW 3 cut(s) 675, 954, 978
PdiI GCCGGC 3 cut(s) 506, 535, 706
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 2 cut(s) 191, 1102
PinAI ACCGGT 1 cut(s) 1107
Ple19I CGATCG 1 cut(s) 807
PleI GAGTC 1 cut(s) 749
PluTI GGCGCC 1 cut(s) 1016
PmaCI CACGTG 2 cut(s) 200, 640
PmlI CACGTG 2 cut(s) 200, 640
PpsI GAGTC 1 cut(s) 749
Ppu21I YACGTR 3 cut(s) 200, 217, 640
PpuMI RGGWCCY 1 cut(s) 235
Psp124BI GAGCTC 3 cut(s) 491, 857, 1037
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 2 cut(s) 615, 695
PspCI CACGTG 2 cut(s) 200, 640
PspGI CCWGG 2 cut(s) 615, 695
PspN4I GGNNCC 5 cut(s) 129, 236, 237, 337, 1014
PspPI GGNCC 5 cut(s) 128, 235, 634, 750, 760
PspPPI RGGWCCY 1 cut(s) 235
PvuI CGATCG 1 cut(s) 807
RsaI GTAC 2 cut(s) 185, 337
RsaNI GTAC 2 cut(s) 184, 336
Rsr2I CGGWCCG 1 cut(s) 750
RsrII CGGWCCG 1 cut(s) 750
SacI GAGCTC 3 cut(s) 491, 857, 1037
SacII CCGCGG 1 cut(s) 823
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 4 cut(s) 369, 443, 804, 969
Sau96I GGNCC 5 cut(s) 128, 235, 634, 750, 760
SchI GAGTC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 579, 617, 697
SduI GDGCHC 5 cut(s) 171, 491, 645, 857, 1037
SfaNI GCATC 2 cut(s) 696, 919
SfoI GGCGCC 1 cut(s) 1014
Sfr303I CCGCGG 1 cut(s) 823
SgrAI CRCCGGYG 2 cut(s) 704, 1107
SgrBI CCGCGG 1 cut(s) 823
SinI GGWCC 5 cut(s) 128, 235, 634, 750, 760
Sse9I AATT 8 cut(s) 79, 145, 245, 314, 557, 847, 870, 884
SspDI GGCGCC 1 cut(s) 1012
SspMI CTAG 3 cut(s) 65, 141, 389
SstI GAGCTC 3 cut(s) 491, 857, 1037
StyD4I CCNGG 3 cut(s) 577, 615, 695
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 183, 592
TaiI ACGT 4 cut(s) 189, 202, 219, 642
TaqI TCGA 4 cut(s) 189, 627, 690, 851
TasI AATT 8 cut(s) 79, 145, 245, 314, 557, 847, 870, 884
TfiI GAWTC 2 cut(s) 191, 1102
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 3 cut(s) 429, 810, 1062
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 1 cut(s) 373
TspGWI ACGGA 2 cut(s) 446, 1091
VpaK11BI GGWCC 5 cut(s) 128, 235, 634, 750, 760
XapI RAATTY 4 cut(s) 145, 245, 314, 557
XmiI GTMKAC 1 cut(s) 1007
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 3 cut(s) 65, 141, 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.