Rw2G013920

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013592
Physical Location & Seq
Forward (+)
15147127 .. 15148098
972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013920.1

Sequence Viewer

Length: 972 bp
ATGGGCTACCGGATAGTGCCCAAGCTTTTACTGTACGTCGATTGCACACGTGCCATTTTCTCATACGTTCTAAAGGCTGAAGGGGACCCACTTAAATTTTCCCTCTTTCAAATCCTCGTCGCTTTCCTTCCGCCACTATATATACCTCGCCCCTCCAAACCAAAATTCAAATCTCACAGACGCGGTACCCTAACCACCATGTTATCGTCGGCGATTGTGATCCTCGTCCTCTTCATCCTAGCCGTCGTTGCTTTGTTCCTCTCCTACTACCTCCGCCGCAACCGCCGCCTCAGATCACAACCTCTCCGTCAACCAGACGCCGCCGCGCCTGTCCAGAGCTCACACTCTCGCCGCCGGCAAGTCTCTCCTGCGCCTTCCTCCTCCGGCCTCCTCCTCCCGCGCTTCAAATTCTCCGACCTGAAACGCCCCGGCTCCGTCACCGTCGGCGGAGACTGCGCGATTTGCCTGGCGGAGTTCGAGCCGGACCACGTGCTCCGCCTCCTCCCTCTCTGCCTCCACGCCTTCCACGAGGATTGCATCGACACCTGGCTCGCCGGCGAGTGGTTGATTTGTCCGGTTTGCCGCGCCGAGGTCTTCCGGTCCGACTCGGACCTCCTCCAGACAGCCTCGTCCGGCGGAAGCTTCCGGCTGGCGATCGGCAGCGTCAGCCGCGGACTGCGGCCAGACACGGCGGCCAATTCGAGCTCGCAAACTCACTCAATTCGCGGAGGTGAATTGGAGTATGTTGTGGATGAGGAGTGGGAGCTTCAACTCAGAGATGCGGTTCTTCCTGTTCACGACGAAGGCGAGGATAACGAGATCGTCGCCGTCGTTACCGACAGCCAACCTCCGCCGTCTACGGCGCCAACTTTGGAGTATCAGAGCTCCTGCGGCATGATTTTCACAGGGAGCAGCCGTCGTTGGACGGAGTTATCCGAGGGACTGGAAATGATTCACCGGTGGCTATCCTGA

Protein Analysis

323

Amino Acids

36.01

Weight (kDa)

7.62

Isoelectric Point (pI)

65.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 151 - 195 7.4e-11 Ring finger domain
zf-RING_11 PF17123 151 - 180 2.6e-09 RING-like zinc finger
zf-C3HC4 PF00097 152 - 194 2.1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 152 - 194 7.4e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 628
Acc65I GGTACC 1 cut(s) 185
AccB1I GGYRCC 2 cut(s) 185, 862
AccI GTMKAC 1 cut(s) 857
AccII CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
AclWI GGATC 1 cut(s) 214
AcoI YGGCCR 2 cut(s) 680, 693
AcsI RAATTY 3 cut(s) 95, 164, 407
AcuI CTGAAG 1 cut(s) 99
AcvI CACGTG 2 cut(s) 50, 490
AcyI GRCGYC 2 cut(s) 318, 863
AfaI GTAC 2 cut(s) 35, 187
AfiI CCNNNNNNNGG 2 cut(s) 561, 589
AflIII ACRYGT 1 cut(s) 47
AgeI ACCGGT 1 cut(s) 957
AgsI TTSAA 4 cut(s) 110, 169, 406, 770
AjnI CCWGG 2 cut(s) 465, 545
AjuI GAANNNNNNNTTGG 2 cut(s) 149, 181
AluBI AGCT 6 cut(s) 25, 339, 642, 705, 766, 885
AluI AGCT 6 cut(s) 25, 339, 642, 705, 766, 885
Alw21I GWGCWC 4 cut(s) 341, 495, 707, 887
Alw26I GTCTC 2 cut(s) 367, 444
AlwI GGATC 1 cut(s) 214
AoxI GGCC 3 cut(s) 385, 680, 693
ApeKI GCWGC 2 cut(s) 660, 912
ApoI RAATTY 3 cut(s) 95, 164, 407
AsiGI ACCGGT 1 cut(s) 957
Asp700I GAANNNNTTC 1 cut(s) 951
Asp718I GGTACC 1 cut(s) 185
AspLEI GCGC 6 cut(s) 328, 373, 402, 458, 587, 865
AspS9I GGNCC 4 cut(s) 85, 484, 600, 610
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 3 cut(s) 430, 743, 947
AvaII GGWCC 4 cut(s) 85, 484, 600, 610
BaeGI GKGCMC 1 cut(s) 21
BanI GGYRCC 2 cut(s) 185, 862
BanII GRGCYC 3 cut(s) 341, 707, 887
BauI CACGAG 1 cut(s) 527
BbrPI CACGTG 2 cut(s) 50, 490
BbsI GAAGAC 1 cut(s) 586
Bbv12I GWGCWC 4 cut(s) 341, 495, 707, 887
BbvI GCAGC 2 cut(s) 672, 924
BceAI ACGGC 6 cut(s) 227, 705, 812, 838, 876, 900
BciT130I CCWGG 2 cut(s) 467, 547
BcnI CCSGG 1 cut(s) 429
BcoDI GTCTC 2 cut(s) 367, 444
BfaI CTAG 1 cut(s) 239
BfoI RGCGCY 1 cut(s) 866
Bme1390I CCNGG 3 cut(s) 429, 467, 547
Bme18I GGWCC 4 cut(s) 85, 484, 600, 610
BmgT120I GGNCC 4 cut(s) 85, 484, 600, 610
BmiI GGNNCC 5 cut(s) 86, 87, 187, 433, 864
BmrFI CCNGG 3 cut(s) 429, 467, 547
BmsI GCATC 2 cut(s) 546, 769
BpiI GAAGAC 1 cut(s) 586
BpmI CTGGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 429
BsaAI YACGTR 2 cut(s) 50, 490
BsaBI GATNNNNATC 1 cut(s) 218
BsaHI GRCGYC 2 cut(s) 318, 863
BsaJI CCNNGG 4 cut(s) 427, 588, 670, 936
BsaWI WCCGGW 4 cut(s) 9, 574, 597, 957
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 901, 931
Bsc4I CCNNNNNNNGG 2 cut(s) 561, 589
Bse118I RCCGGY 3 cut(s) 354, 554, 957
Bse1I ACTGG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 218
BseBI CCWGG 2 cut(s) 467, 547
BseDI CCNNGG 4 cut(s) 427, 588, 670, 936
BseGI GGATG 2 cut(s) 234, 757
BseJI GATNNNNATC 1 cut(s) 218
BseLI CCNNNNNNNGG 2 cut(s) 561, 589
BseMII CTCAG 2 cut(s) 304, 787
BseNI ACTGG 1 cut(s) 948
BseRI GAGGAG 6 cut(s) 370, 380, 383, 491, 605, 770
BseSI GKGCMC 1 cut(s) 21
BseXI GCAGC 2 cut(s) 672, 924
Bsh1236I CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
Bsh1285I CGRYCG 1 cut(s) 657
BshFI GGCC 3 cut(s) 387, 682, 695
BshNI GGYRCC 2 cut(s) 185, 862
BshTI ACCGGT 1 cut(s) 957
BsiEI CGRYCG 1 cut(s) 657
BsiHKAI GWGCWC 4 cut(s) 341, 495, 707, 887
BslFI GGGAC 2 cut(s) 98, 954
BslI CCNNNNNNNGG 2 cut(s) 561, 589
BsmAI GTCTC 2 cut(s) 367, 444
BsmFI GGGAC 2 cut(s) 98, 954
BsnI GGCC 3 cut(s) 387, 682, 695
Bsp1286I GDGCHC 5 cut(s) 21, 341, 495, 707, 887
Bsp143I GATC 4 cut(s) 219, 293, 654, 819
BspANI GGCC 3 cut(s) 387, 682, 695
BspCNI CTCAG 2 cut(s) 303, 786
BspFNI CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
BspLI GGNNCC 5 cut(s) 86, 87, 187, 433, 864
BspPI GGATC 1 cut(s) 214
BspT107I GGYRCC 2 cut(s) 185, 862
BsrFI RCCGGY 3 cut(s) 354, 554, 957
BsrI ACTGG 1 cut(s) 948
BssAI RCCGGY 3 cut(s) 354, 554, 957
BssECI CCNNGG 4 cut(s) 427, 588, 670, 936
BssMI GATC 4 cut(s) 219, 293, 654, 819
BssNI GRCGYC 2 cut(s) 318, 863
BssSI CACGAG 1 cut(s) 527
Bst2BI CACGAG 1 cut(s) 527
Bst2UI CCWGG 2 cut(s) 467, 547
Bst4CI ACNGT 2 cut(s) 33, 442
Bst6I CTCTTC 1 cut(s) 236
BstACI GRCGYC 2 cut(s) 318, 863
BstBAI YACGTR 2 cut(s) 50, 490
BstC8I GCNNGC 5 cut(s) 356, 552, 556, 651, 707
BstDEI CTNAG 2 cut(s) 290, 773
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 2 cut(s) 234, 757
BstFNI CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 6 cut(s) 328, 373, 402, 458, 587, 865
BstKTI GATC 4 cut(s) 222, 296, 657, 822
BstMAI GTCTC 2 cut(s) 367, 444
BstMBI GATC 4 cut(s) 219, 293, 654, 819
BstMCI CGRYCG 1 cut(s) 657
BstMWI GCNNNNNNNGC 8 cut(s) 248, 282, 285, 453, 462, 666, 669, 891
BstNI CCWGG 2 cut(s) 467, 547
BstSCI CCNGG 3 cut(s) 427, 465, 545
BstSLI GKGCMC 1 cut(s) 21
BstUI CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
BstV1I GCAGC 2 cut(s) 672, 924
BstV2I GAAGAC 1 cut(s) 586
BsuRI GGCC 3 cut(s) 387, 682, 695
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 2 cut(s) 234, 757
Cac8I GCNNGC 5 cut(s) 356, 552, 556, 651, 707
CfoI GCGC 6 cut(s) 328, 373, 402, 458, 587, 865
Cfr10I RCCGGY 3 cut(s) 354, 554, 957
Cfr13I GGNCC 4 cut(s) 85, 484, 600, 610
Cfr42I CCGCGG 1 cut(s) 673
CpoI CGGWCCG 1 cut(s) 600
CseI GACGC 3 cut(s) 189, 326, 652
Csp6I GTAC 2 cut(s) 34, 186
CspAI ACCGGT 1 cut(s) 957
CspCI CAANNNNNGTGG 2 cut(s) 515, 550
CspI CGGWCCG 1 cut(s) 600
CviAII CATG 2 cut(s) 199, 895
CviQI GTAC 2 cut(s) 34, 186
DdeI CTNAG 2 cut(s) 290, 773
DinI GGCGCC 1 cut(s) 864
DpnI GATC 4 cut(s) 221, 295, 656, 821
DpnII GATC 4 cut(s) 219, 293, 654, 819
DrdI GACNNNNNNGTC 1 cut(s) 628
DseDI GACNNNNNNGTC 1 cut(s) 628
EaeI YGGCCR 2 cut(s) 680, 693
Eam1104I CTCTTC 1 cut(s) 236
EarI CTCTTC 1 cut(s) 236
EciI GGCGGA 7 cut(s) 120, 263, 462, 485, 485, 651, 840
Ecl136II GAGCTC 3 cut(s) 339, 705, 885
Eco24I GRGCYC 3 cut(s) 341, 707, 887
Eco47I GGWCC 4 cut(s) 85, 484, 600, 610
Eco53kI GAGCTC 3 cut(s) 339, 705, 885
Eco57I CTGAAG 1 cut(s) 99
Eco72I CACGTG 2 cut(s) 50, 490
EcoICRI GAGCTC 3 cut(s) 339, 705, 885
EcoO109I RGGNCCY 1 cut(s) 85
EcoRII CCWGG 2 cut(s) 465, 545
EcoT38I GRGCYC 3 cut(s) 341, 707, 887
EgeI GGCGCC 1 cut(s) 864
EheI GGCGCC 1 cut(s) 864
FaeI CATG 2 cut(s) 202, 898
FaiI YATR 7 cut(s) 64, 139, 141, 143, 200, 744, 896
FaqI GGGAC 2 cut(s) 98, 954
FatI CATG 2 cut(s) 198, 894
FauI CCCGC 1 cut(s) 405
FblI GTMKAC 1 cut(s) 857
FokI GGATG 2 cut(s) 221, 764
FriOI GRGCYC 3 cut(s) 341, 707, 887
FspBI CTAG 1 cut(s) 239
GlaI GCGC 6 cut(s) 327, 372, 401, 457, 586, 864
GsuI CTGGAG 1 cut(s) 602
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 3 cut(s) 387, 682, 695
HgaI GACGC 3 cut(s) 189, 326, 652
HhaI GCGC 6 cut(s) 328, 373, 402, 458, 587, 865
Hin1I GRCGYC 2 cut(s) 318, 863
Hin1II CATG 2 cut(s) 202, 898
Hin6I GCGC 6 cut(s) 326, 371, 400, 456, 585, 863
HinP1I GCGC 6 cut(s) 326, 371, 400, 456, 585, 863
HincII GTYRAC 1 cut(s) 311
HindII GTYRAC 1 cut(s) 311
HindIII AAGCTT 2 cut(s) 23, 640
HinfI GANTC 2 cut(s) 605, 952
HphI GGTGA 3 cut(s) 430, 743, 947
Hpy166II GTNNAC 3 cut(s) 311, 796, 858
Hpy188I TCNGA 7 cut(s) 293, 415, 604, 610, 776, 882, 937
Hpy188III TCNNGA 4 cut(s) 334, 619, 797, 969
Hpy8I GTNNAC 3 cut(s) 311, 796, 858
Hpy99I CGWCG 9 cut(s) 41, 122, 211, 248, 446, 803, 827, 833, 921
HpyAV CCTTC 5 cut(s) 74, 137, 384, 532, 797
HpyCH4III ACNGT 2 cut(s) 33, 442
HpyCH4IV ACGT 4 cut(s) 36, 49, 66, 489
HpyCH4V TGCA 2 cut(s) 45, 537
HpyF10VI GCNNNNNNNGC 8 cut(s) 248, 282, 285, 453, 462, 666, 669, 891
HpyF3I CTNAG 2 cut(s) 290, 773
HpySE526I ACGT 4 cut(s) 36, 49, 66, 489
Hsp92I GRCGYC 2 cut(s) 318, 863
Hsp92II CATG 2 cut(s) 202, 898
HspAI GCGC 6 cut(s) 326, 371, 400, 456, 585, 863
KasI GGCGCC 1 cut(s) 862
KflI GGGWCCC 1 cut(s) 85
KpnI GGTACC 1 cut(s) 189
KroI GCCGGC 2 cut(s) 354, 554
KroNI GCCGGC 2 cut(s) 356, 556
KspI CCGCGG 1 cut(s) 673
Kzo9I GATC 4 cut(s) 219, 293, 654, 819
LmnI GCTCC 5 cut(s) 437, 498, 763, 890, 909
Lsp1109I GCAGC 2 cut(s) 672, 924
LweI GCATC 2 cut(s) 546, 769
MaeI CTAG 1 cut(s) 239
MaeII ACGT 4 cut(s) 36, 49, 66, 489
MaeIII GTNAC 2 cut(s) 436, 832
MalI GATC 4 cut(s) 221, 295, 656, 821
MboI GATC 4 cut(s) 219, 293, 654, 819
MboII GAAGA 3 cut(s) 223, 586, 779
MhlI GDGCHC 5 cut(s) 21, 341, 495, 707, 887
MluCI AATT 6 cut(s) 95, 164, 407, 697, 720, 734
Mly113I GGCGCC 1 cut(s) 863
MlyI GAGTC 1 cut(s) 599
MmeI TCCRAC 3 cut(s) 438, 627, 902
MreI CGCCGGCG 1 cut(s) 554
MroNI GCCGGC 2 cut(s) 354, 554
MroXI GAANNNNTTC 1 cut(s) 951
MseI TTAA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 672
MspR9I CCNGG 3 cut(s) 429, 467, 547
MvaI CCWGG 2 cut(s) 467, 547
MvnI CGCG 7 cut(s) 183, 326, 400, 458, 585, 672, 726
MwoI GCNNNNNNNGC 8 cut(s) 248, 282, 285, 453, 462, 666, 669, 891
NaeI GCCGGC 2 cut(s) 356, 556
NarI GGCGCC 1 cut(s) 863
NciI CCSGG 1 cut(s) 429
NdeII GATC 4 cut(s) 219, 293, 654, 819
NgoMIV GCCGGC 2 cut(s) 354, 554
NlaIII CATG 2 cut(s) 202, 898
NlaIV GGNNCC 5 cut(s) 86, 87, 187, 433, 864
NmeAIII GCCGAG 1 cut(s) 613
NmuCI GTSAC 1 cut(s) 436
PcsI WCGNNNNNNNCGW 3 cut(s) 525, 804, 828
PdiI GCCGGC 2 cut(s) 356, 556
PdmI GAANNNNTTC 1 cut(s) 951
PfeI GAWTC 1 cut(s) 952
PinAI ACCGGT 1 cut(s) 957
Ple19I CGATCG 1 cut(s) 657
PleI GAGTC 1 cut(s) 599
PluTI GGCGCC 1 cut(s) 866
PmaCI CACGTG 2 cut(s) 50, 490
PmlI CACGTG 2 cut(s) 50, 490
PpsI GAGTC 1 cut(s) 599
Ppu21I YACGTR 2 cut(s) 50, 490
PpuMI RGGWCCY 1 cut(s) 85
Psp124BI GAGCTC 3 cut(s) 341, 707, 887
Psp5II RGGWCCY 1 cut(s) 85
Psp6I CCWGG 2 cut(s) 465, 545
PspCI CACGTG 2 cut(s) 50, 490
PspGI CCWGG 2 cut(s) 465, 545
PspN4I GGNNCC 5 cut(s) 86, 87, 187, 433, 864
PspPI GGNCC 4 cut(s) 85, 484, 600, 610
PspPPI RGGWCCY 1 cut(s) 85
PvuI CGATCG 1 cut(s) 657
RsaI GTAC 2 cut(s) 35, 187
RsaNI GTAC 2 cut(s) 34, 186
Rsr2I CGGWCCG 1 cut(s) 600
RsrII CGGWCCG 1 cut(s) 600
SacI GAGCTC 3 cut(s) 341, 707, 887
SacII CCGCGG 1 cut(s) 673
SaqAI TTAA 1 cut(s) 93
Sau3AI GATC 4 cut(s) 219, 293, 654, 819
Sau96I GGNCC 4 cut(s) 85, 484, 600, 610
SchI GAGTC 1 cut(s) 599
ScrFI CCNGG 3 cut(s) 429, 467, 547
SduI GDGCHC 5 cut(s) 21, 341, 495, 707, 887
SfaNI GCATC 2 cut(s) 546, 769
SfoI GGCGCC 1 cut(s) 864
Sfr303I CCGCGG 1 cut(s) 673
SgrAI CRCCGGYG 2 cut(s) 554, 957
SgrBI CCGCGG 1 cut(s) 673
SinI GGWCC 4 cut(s) 85, 484, 600, 610
Sse9I AATT 6 cut(s) 95, 164, 407, 697, 720, 734
SspDI GGCGCC 1 cut(s) 862
SspMI CTAG 1 cut(s) 239
SstI GAGCTC 3 cut(s) 341, 707, 887
StyD4I CCNGG 3 cut(s) 427, 465, 545
TaaI ACNGT 2 cut(s) 33, 442
TaiI ACGT 4 cut(s) 39, 52, 69, 492
TaqI TCGA 4 cut(s) 39, 477, 540, 701
TasI AATT 6 cut(s) 95, 164, 407, 697, 720, 734
TfiI GAWTC 1 cut(s) 952
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseFI GTSAC 1 cut(s) 436
TseI GCWGC 2 cut(s) 660, 912
Tsp45I GTSAC 1 cut(s) 436
TspDTI ATGAA 1 cut(s) 223
TspGWI ACGGA 3 cut(s) 296, 424, 941
VpaK11BI GGWCC 4 cut(s) 85, 484, 600, 610
XapI RAATTY 3 cut(s) 95, 164, 407
XmiI GTMKAC 1 cut(s) 857
XmnI GAANNNNTTC 1 cut(s) 951
XspI CTAG 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.