Rmu_sc0001235.1_g000030

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001235.1
OG0007159
Physical Location & Seq
Reverse (-)
148229 .. 155962
7734 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4035 bp
atgggacagcttccttcgcaggatatattggcgctgctcgcattcaagaagggtgtcaagcatgatcctactggttttgttgtcaattcttggaatgatgagtccattgacttcaatggttgtccttcttcttggaatggggttatatgtaatggtggaaatgttgctggagttgtccttgataatttgagtctctctgcggatgtggacttgagtgtgttttcgaatctaaccaagcttctaaaactttccatggccaacaattcgatatctgggaaaattcctgacaatattgcagacttcaacagcctccagtttcttgatctctccaacaatttatttgcttcatctttaccacctggaattggtaagttggggagcttaaggaacctctcattagctggcaataacttctcgggctccattccagattccatttcagggctttcctcaatccagtcgttggactttagtcgcaactccttttcagggccgctgccgtctgcattgacaaagctgtctagtctggtatctctaaatctttctttgaatggctttaccaagagcttacctaaggggtttgagctaatgtcaagtctagatttgctcgacttgcatggaaatatgcttgatggtcctctggataaggcatttctgatggaagcaactgccacgcatgttgattttagtggcaatatgtttacaagttccagttcacagcagcagatgtttctacctcgtctttctgagagtatcaagtacttgaatctttcccacaaccagcttactgggtcactggtaggtggcagtgagcttcagatatttgaggacttgaaggtgttggatctcagctacaaccagctgtcaggagaattgcctggatttaattttgtctatgacctccaggtcctcaaacttagcaacaacagatttactggagttgttcccaatggcctaataaaaggagattctctggttttaagtgaactagatctgagtggcaacaatctctcaggaccaataactatggtgacatcaacaactctgagaatccttaatctctcctcgaatgggcttactggtaagttgccattgctgactggaagttgtgctgtacttgatctatccaagaacaaattagagggaaatttaaccaggatggtgaaatgggggaacattgaataccttgatctgagtcaaaatcttttgacaggacccatccctgatgtcactccacagtttttgcgtttaaattatctcaacctctcccataattctcttagtagttccatagcaagtgttataacacagtatccaaagattagtgtccttgatctcagttctaaccagttagatggcacagttctagctgagttgctaagcatgcctactctgcaagagctccaccttgaaaataatttactcactgggtctatcaacatttcatctcctttgttcaaccaatccaatcttcaggttcttgatctttctcagaaccggctgagtggttattttcctgatcaatttggttcactgaatgggcttaaagtgctcaatattgggagaaataatctttctggctctctgccaacttccatatctgacatgagcacactaagctcattagacatatcgcagaatcatttcacaggtcctcttccaaacaacttgccaaacagtcttgtattctttaatgcttcatataatgatctttctggggatgtcccagaaaatctgagaaagtttccgagttcttccttctatccagggaatacgcgcttgcgctttccaagtggtggtcctcctggatcaaacaattccgaatctgaacattcaaagaggaaaccattcagcacgcttgtcaaagtcatcattatagtttcatgtgtggttgctgttttcattcttcttctgcttggtatcttcattcattacatctgcatgtcaaggagaaccccatcagagaatactgcaaacaaagacatccacaaacgtgctccaccaaacccctcaggtgctcgaggagcagagagtggtggtgctttggttgtttcagctggggatcttgtggcttcacggaaaggatcgtcttctgaaattatcagccctggtgaaaaactcgcagctgtaagtgacttctccccttcaaagaacagccatttctcttggtcaccagagtctggagattcctacactgccgaaaaccttgccagactggacgtgagatcaccggatagattggttggtgagcttcattttcttgatgaaacaattgcattaacacctgaggagctttcaagggctcctgctgaagttttggggagaagcagccatggcacttcttataaggcaacacttgataacgggctgttcttgacagttaagtggttgagagaaggggtggcaaagcagaaaaaggagtttgccaaggaggctaagaaatttgccaatatgaggcatccaaatgttgtgggtttgagagggtactattggggtcctacacagcatgagaagcttattctttcagattatatctcacctggaagtcttgcaagctttctttatgatcgaccaggaagaaaaggtccaccattgacctgggcccaaaggctcaaaattgcagttgatgttgcacgtggcctgaactatctccattttgatcgtgctgttccgcacggtaacctcaaagcaacaaatattctgttggatgggtctgatctgaatgcccgagttgctgattactgcctccaccgcctcatgacacaggctggcaccattgaacagattcttgatgctggtgtcttaggttaccgcgccccagagttggctgcttccaagaagccactgccctccttcaagtccgatgtttatgcctttggagtgatattgttggaacttttaactggaaggtgcgctggtgatgtgatctctggtgaaggaggcggggcagatttgacagaatgggtgcggttgagggtagcagaaggtcgtgggtcagattgttttgatgctgcattggtgactgagatgggaaatccagcagcagagaagggaatgaaggaagtccttggaatatcattacggtgtatacgttctgtttctgagaggccagggtttattggactgggaaatatgggatccagaatggcaaacaatctaataaaggcgggataccgagtagctgttcatgacataaactgcaatgccatgaaaaagttctctgatattggagccgcaacaaaagaaacacctactgaagttgcagaagctagtgatgttgtaatcacaatgctccctacatcatctcatgtatatgatgtttatactggaccaaatggtttactttatggagggaatttgcgaccacgtctattgatagattcatccactattgatccacaaacatcgagaaagctttctaccatcgtctctaactgtactctaaaggaacagaaagaagatcattggaagaagcctgccttgctggatgctcctgtatctggaggtgtccttgcagcggaagctgggactcttactttcatggttggtggatctgaggaagcttatatggcggctgaacccttgttcctttcgatggggaagaacacagtttattgtggtggggcaggaaatggttcagcagcaaagatttgcaataatctggcactggctataagtatgattggcgtctcagaatctcttgctcttggtcagtctctaggaatatctgccagtactctaacaaaagtgtttaactcttccagtgctcgctgttggagcagcgatagttacaatccagttccgggagtgatggaaggggttccctcttcgagaaactatgatggtgggtttgcatccaaactaatggctaaagacgtaaatattgctgcaacatcagccaaagaggttggtcttcgatgcccgttgacttcccaagcacaagagatatatacagagctctgtgaggatggccatgaaaccaaggacttctcctgcgtattccgccattattactctggcaagaatgagatttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase_Tyr

Protein Analysis

1344

Amino Acids

144.16

Weight (kDa)

6.14

Isoelectric Point (pI)

36.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 6 - 51 3.3e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 122 - 206 2.7e-07 Leucine-rich repeat region
LRR_8 PF13855 489 - 549 3.3e-11 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 771 - 968 6.8e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 771 - 970 1.8e-24 Protein kinase domain
2-Hacid_dh_C PF02826 1036 - 1103 8.1e-06 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
NAD_binding_2 PF03446 1038 - 1207 8.7e-40 NAD binding domain of 6-phosphogluconate dehydrogenase
NAD_binding_11 PF14833 1210 - 1336 2.1e-26 NAD-binding of NADP-dependent 3-hydroxyisobutyrate dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1304, 2346
AasI GACNNNNNNGTC 2 cut(s) 517, 905
AccB1I GGYRCC 1 cut(s) 2771
AccB7I CCANNNNNTGG 3 cut(s) 463, 1796, 2180
AccI GTMKAC 1 cut(s) 3088
AccII CGCG 2 cut(s) 1777, 2814
AclWI GGATC 9 cut(s) 59, 852, 1816, 2070, 2092, 3132, 3145, 3389, 3559
AcoI YGGCCR 2 cut(s) 255, 3970
AcsI RAATTY 4 cut(s) 279, 1147, 2441, 3355
AcuI CTGAAG 4 cut(s) 800, 1457, 2331, 3276
AcvI CACGTG 1 cut(s) 2636
AcyI GRCGYC 1 cut(s) 3687
AfaI GTAC 5 cut(s) 761, 1116, 2486, 3439, 3736
AflII CTTAAG 1 cut(s) 382
AjiI CACGTC 2 cut(s) 2221, 3368
AjuI GAANNNNNNNTTGG 2 cut(s) 1455, 1487
AleI CACNNNNGTG 1 cut(s) 1992
AloI GAACNNNNNNTCC 2 cut(s) 3570, 3602
Alw21I GWGCWC 7 cut(s) 1404, 1554, 1613, 1999, 2020, 3769, 3960
Alw26I GTCTC 4 cut(s) 197, 3433, 3694, 3720
AlwI GGATC 9 cut(s) 59, 852, 1816, 2070, 2092, 3132, 3145, 3389, 3559
AlwNI CAGNNNCTG 1 cut(s) 2180
Ama87I CYCGRG 3 cut(s) 415, 2019, 2727
AoxI GGCC 7 cut(s) 255, 491, 952, 2601, 2638, 3107, 3970
ApaI GGGCCC 1 cut(s) 2605
ApoI RAATTY 4 cut(s) 279, 1147, 2441, 3355
Asp700I GAANNNNTTC 7 cut(s) 1643, 1847, 2784, 3215, 3415, 3634, 3819
AspLEI GCGC 5 cut(s) 34, 1779, 1785, 2816, 2915
AsuC2I CCSGG 1 cut(s) 3804
AsuII TTCGAA 1 cut(s) 224
AvaI CYCGRG 3 cut(s) 415, 2019, 2727
AvaII GGWCC 9 cut(s) 635, 907, 1016, 1214, 1652, 1799, 2495, 2585, 3329
AxyI CCTNAGG 3 cut(s) 573, 2011, 2286
BaeGI GKGCMC 1 cut(s) 2605
BalI TGGCCA 2 cut(s) 257, 3972
BamHI GGATCC 1 cut(s) 3137
BanI GGYRCC 1 cut(s) 2771
BanII GRGCYC 5 cut(s) 422, 1404, 2305, 2605, 3960
BbrPI CACGTG 1 cut(s) 2636
BbsI GAAGAC 2 cut(s) 2082, 3905
Bbv12I GWGCWC 7 cut(s) 1404, 1554, 1613, 1999, 2020, 3769, 3960
BceAI ACGGC 1 cut(s) 484
BcgI CGANNNNNNTGC 4 cut(s) 2189, 2223, 2852, 2886
BciVI GTATCC 2 cut(s) 1323, 3164
BclI TGATCA 1 cut(s) 1519
BcnI CCSGG 1 cut(s) 3804
BcoDI GTCTC 4 cut(s) 197, 3433, 3694, 3720
BfaI CTAG 6 cut(s) 522, 599, 989, 1367, 3270, 3719
BfoI RGCGCY 1 cut(s) 35
BfrI CTTAAG 1 cut(s) 382
BfuI GTATCC 2 cut(s) 1323, 3164
BglI GCCNNNNNGGC 1 cut(s) 2432
BglII AGATCT 1 cut(s) 991
BlpI GCTNAGC 1 cut(s) 1379
BmcAI AGTACT 2 cut(s) 761, 3736
Bme18I GGWCC 9 cut(s) 635, 907, 1016, 1214, 1652, 1799, 2495, 2585, 3329
BmeT110I CYCGRG 3 cut(s) 415, 2019, 2727
BmgBI CACGTC 2 cut(s) 2221, 3368
BmrI ACTGGG 3 cut(s) 798, 1437, 3134
BmsI GCATC 6 cut(s) 2467, 2782, 2998, 3478, 3863, 3908
BmuI ACTGGG 3 cut(s) 798, 1437, 3134
BoxI GACNNNNGTC 1 cut(s) 471
BpiI GAAGAC 2 cut(s) 2082, 3905
BpmI CTGGAG 6 cut(s) 189, 296, 887, 957, 2202, 3522
Bpu1102I GCTNAGC 1 cut(s) 1379
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 232
BpuMI CCSGG 1 cut(s) 3804
BsaAI YACGTR 1 cut(s) 2636
BsaHI GRCGYC 1 cut(s) 3687
BsaJI CCNNGG 9 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067, 3110, 3981
BsaWI WCCGGW 1 cut(s) 2230
Bse118I RCCGGY 1 cut(s) 1497
Bse21I CCTNAGG 3 cut(s) 573, 2011, 2286
Bse3DI GCAATG 2 cut(s) 1091, 3208
BseDI CCNNGG 9 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067, 3110, 3981
BseMI GCAATG 2 cut(s) 1091, 3208
BseRI GAGGAG 3 cut(s) 1054, 2037, 2303
BseSI GKGCMC 1 cut(s) 2605
BseYI CCCAGC 2 cut(s) 2057, 3524
Bsh1236I CGCG 2 cut(s) 1777, 2814
BshFI GGCC 7 cut(s) 257, 493, 954, 2603, 2640, 3109, 3972
BshNI GGYRCC 1 cut(s) 2771
BsiHKAI GWGCWC 7 cut(s) 1404, 1554, 1613, 1999, 2020, 3769, 3960
BsiHKCI CYCGRG 3 cut(s) 415, 2019, 2727
BsiSI CCGG 3 cut(s) 1498, 2231, 3803
BslFI GGGAC 3 cut(s) 18, 1709, 3541
BsmAI GTCTC 4 cut(s) 197, 3433, 3694, 3720
BsmBI CGTCTC 2 cut(s) 3433, 3694
BsmFI GGGAC 3 cut(s) 18, 1709, 3541
BsmI GAATGC 2 cut(s) 41, 2728
BsnI GGCC 7 cut(s) 257, 493, 954, 2603, 2640, 3109, 3972
BsoBI CYCGRG 3 cut(s) 415, 2019, 2727
Bsp119I TTCGAA 1 cut(s) 224
Bsp120I GGGCCC 1 cut(s) 2601
Bsp1720I GCTNAGC 1 cut(s) 1379
Bsp19I CCATGG 2 cut(s) 252, 2332
BspANI GGCC 7 cut(s) 257, 493, 954, 2603, 2640, 3109, 3972
BspFNI CGCG 2 cut(s) 1777, 2814
BspHI TCATGA 2 cut(s) 2757, 3187
BspPI GGATC 9 cut(s) 59, 852, 1816, 2070, 2092, 3132, 3145, 3389, 3559
BspT104I TTCGAA 1 cut(s) 224
BspT107I GGYRCC 1 cut(s) 2771
BspTI CTTAAG 1 cut(s) 382
BsrDI GCAATG 2 cut(s) 1091, 3208
BsrFI RCCGGY 1 cut(s) 1497
BssAI RCCGGY 1 cut(s) 1497
BssECI CCNNGG 9 cut(s) 252, 1766, 2107, 2332, 2427, 2598, 3067, 3110, 3981
BssNAI GTATAC 1 cut(s) 3089
BssNI GRCGYC 1 cut(s) 3687
BssT1I CCWWGG 5 cut(s) 252, 2332, 2427, 3067, 3981
Bst1107I GTATAC 1 cut(s) 3089
Bst4CI ACNGT 9 cut(s) 1239, 1311, 1363, 1679, 2380, 2678, 3084, 3437, 3610
Bst6I CTCTTC 3 cut(s) 1662, 3763, 3832
BstACI GRCGYC 1 cut(s) 3687
BstAFI CTTAAG 1 cut(s) 382
BstBAI YACGTR 1 cut(s) 2636
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 9 cut(s) 39, 403, 1385, 1781, 1856, 2554, 2770, 3477, 3769
BstDSI CCRYGG 2 cut(s) 252, 2332
BstEII GGTNACC 3 cut(s) 2169, 2678, 2807
BstFNI CGCG 2 cut(s) 1777, 2814
BstH2I RGCGCY 1 cut(s) 35
BstHHI GCGC 5 cut(s) 34, 1779, 1785, 2816, 2915
BstMAI GTCTC 4 cut(s) 197, 3433, 3694, 3720
BstNSI RCATGY 3 cut(s) 680, 1387, 1945
BstPAI GACNNNNGTC 1 cut(s) 471
BstPI GGTNACC 3 cut(s) 2169, 2678, 2807
BstSLI GKGCMC 1 cut(s) 2605
BstUI CGCG 2 cut(s) 1777, 2814
BstV2I GAAGAC 2 cut(s) 2082, 3905
BstX2I RGATCY 5 cut(s) 844, 991, 2062, 3137, 3551
BstXI CCANNNNNNTGG 4 cut(s) 788, 1355, 2598, 3865
BstYI RGATCY 5 cut(s) 844, 991, 2062, 3137, 3551
BstZ17I GTATAC 1 cut(s) 3089
Bsu36I CCTNAGG 3 cut(s) 573, 2011, 2286
BsuI GTATCC 2 cut(s) 1323, 3164
BsuRI GGCC 7 cut(s) 257, 493, 954, 2603, 2640, 3109, 3972
BtgI CCRYGG 2 cut(s) 252, 2332
BtrI CACGTC 2 cut(s) 2221, 3368
BtsI GCAGTG 3 cut(s) 814, 2193, 2843
BtsIMutI CAGTG 8 cut(s) 794, 814, 1425, 1532, 2193, 2843, 3665, 3769
Cac8I GCNNGC 9 cut(s) 39, 403, 1385, 1781, 1856, 2554, 2770, 3477, 3769
CaiI CAGNNNCTG 1 cut(s) 2180
CciI TCATGA 2 cut(s) 2757, 3187
CfoI GCGC 5 cut(s) 34, 1779, 1785, 2816, 2915
Cfr10I RCCGGY 1 cut(s) 1497
CseI GACGC 1 cut(s) 3676
Csp6I GTAC 5 cut(s) 760, 1115, 2485, 3438, 3735
CspCI CAANNNNNGTGG 8 cut(s) 661, 696, 1224, 1259, 2451, 2486, 3354, 3389
CviQI GTAC 5 cut(s) 760, 1115, 2485, 3438, 3735
DraI TTTAAA 1 cut(s) 1251
DrdI GACNNNNNNGTC 2 cut(s) 517, 905
DseDI GACNNNNNNGTC 2 cut(s) 517, 905
EaeI YGGCCR 2 cut(s) 255, 3970
Eam1104I CTCTTC 3 cut(s) 1662, 3763, 3832
EarI CTCTTC 3 cut(s) 1662, 3763, 3832
EciI GGCGGA 1 cut(s) 3992
Ecl136II GAGCTC 2 cut(s) 1402, 3958
Eco130I CCWWGG 5 cut(s) 252, 2332, 2427, 3067, 3981
Eco24I GRGCYC 5 cut(s) 422, 1404, 2305, 2605, 3960
Eco32I GATATC 1 cut(s) 270
Eco47I GGWCC 9 cut(s) 635, 907, 1016, 1214, 1652, 1799, 2495, 2585, 3329
Eco53kI GAGCTC 2 cut(s) 1402, 3958
Eco57I CTGAAG 4 cut(s) 800, 1457, 2331, 3276
Eco72I CACGTG 1 cut(s) 2636
Eco81I CCTNAGG 3 cut(s) 573, 2011, 2286
Eco88I CYCGRG 3 cut(s) 415, 2019, 2727
Eco91I GGTNACC 3 cut(s) 2169, 2678, 2807
EcoICRI GAGCTC 2 cut(s) 1402, 3958
EcoO109I RGGNCCY 4 cut(s) 907, 1214, 1652, 2495
EcoO65I GGTNACC 3 cut(s) 2169, 2678, 2807
EcoRV GATATC 1 cut(s) 270
EcoT14I CCWWGG 5 cut(s) 252, 2332, 2427, 3067, 3981
EcoT38I GRGCYC 5 cut(s) 422, 1404, 2305, 2605, 3960
ErhI CCWWGG 5 cut(s) 252, 2332, 2427, 3067, 3981
Esp3I CGTCTC 2 cut(s) 3433, 3694
FalI AAGNNNNNCTT 2 cut(s) 3464, 3496
FaqI GGGAC 3 cut(s) 18, 1709, 3541
FauI CCCGC 2 cut(s) 2936, 3160
FbaI TGATCA 1 cut(s) 1519
FblI GTMKAC 1 cut(s) 3088
FriOI GRGCYC 5 cut(s) 422, 1404, 2305, 2605, 3960
FspBI CTAG 6 cut(s) 522, 599, 989, 1367, 3270, 3719
GlaI GCGC 5 cut(s) 33, 1778, 1784, 2815, 2914
GsaI CCCAGC 2 cut(s) 2061, 3528
GsuI CTGGAG 6 cut(s) 189, 296, 887, 957, 2202, 3522
HaeII RGCGCY 1 cut(s) 35
HaeIII GGCC 7 cut(s) 257, 493, 954, 2603, 2640, 3109, 3972
HapII CCGG 3 cut(s) 1498, 2231, 3803
HgaI GACGC 1 cut(s) 3676
HhaI GCGC 5 cut(s) 34, 1779, 1785, 2816, 2915
Hin1I GRCGYC 1 cut(s) 3687
Hin6I GCGC 5 cut(s) 32, 1777, 1783, 2814, 2913
HinP1I GCGC 5 cut(s) 32, 1777, 1783, 2814, 2913
HincII GTYRAC 1 cut(s) 3927
HindII GTYRAC 1 cut(s) 3927
HindIII AAGCTT 5 cut(s) 236, 2513, 2554, 3413, 3561
HpaII CCGG 3 cut(s) 1498, 2231, 3803
Hpy166II GTNNAC 9 cut(s) 208, 702, 716, 986, 1532, 2588, 3089, 3341, 3927
Hpy8I GTNNAC 9 cut(s) 208, 702, 716, 986, 1532, 2588, 3089, 3341, 3927
HpyCH4III ACNGT 9 cut(s) 1239, 1311, 1363, 1679, 2380, 2678, 3084, 3437, 3610
HpyCH4IV ACGT 6 cut(s) 1993, 2220, 2635, 3091, 3367, 3876
HpySE526I ACGT 6 cut(s) 1993, 2220, 2635, 3091, 3367, 3876
Hsp92I GRCGYC 1 cut(s) 3687
HspAI GCGC 5 cut(s) 32, 1777, 1783, 2814, 2913
Ksp22I TGATCA 1 cut(s) 1519
LweI GCATC 6 cut(s) 2467, 2782, 2998, 3478, 3863, 3908
MaeI CTAG 6 cut(s) 522, 599, 989, 1367, 3270, 3719
MaeII ACGT 6 cut(s) 1993, 2220, 2635, 3091, 3367, 3876
MaeIII GTNAC 9 cut(s) 792, 1030, 1228, 2132, 2169, 2678, 2807, 3019, 3788
MfeI CAATTG 1 cut(s) 2271
MflI RGATCY 5 cut(s) 844, 991, 2062, 3137, 3551
MlsI TGGCCA 2 cut(s) 257, 3972
MluNI TGGCCA 2 cut(s) 257, 3972
MlyI GAGTC 5 cut(s) 110, 199, 1204, 2186, 3523
MmeI TCCRAC 6 cut(s) 354, 444, 822, 2685, 2871, 3755
Mox20I TGGCCA 2 cut(s) 257, 3972
MroXI GAANNNNTTC 7 cut(s) 1643, 1847, 2784, 3215, 3415, 3634, 3819
MscI TGGCCA 2 cut(s) 257, 3972
MslI CAYNNNNRTG 5 cut(s) 1940, 1992, 2463, 3082, 3312
Msp20I TGGCCA 2 cut(s) 257, 3972
MspA1I CMGCKG 5 cut(s) 496, 862, 2057, 2126, 3518
MspCI CTTAAG 1 cut(s) 382
MspI CCGG 3 cut(s) 1498, 2231, 3803
MunI CAATTG 1 cut(s) 2271
Mva1269I GAATGC 2 cut(s) 41, 2728
MvnI CGCG 2 cut(s) 1777, 2814
NciI CCSGG 1 cut(s) 3804
NcoI CCATGG 2 cut(s) 252, 2332
NmuCI GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
NspI RCATGY 3 cut(s) 680, 1387, 1945
NspV TTCGAA 1 cut(s) 224
OliI CACNNNNGTG 1 cut(s) 1992
PaeI GCATGC 1 cut(s) 1387
PaeR7I CTCGAG 1 cut(s) 2019
PagI TCATGA 2 cut(s) 2757, 3187
PcsI WCGNNNNNNNCGW 1 cut(s) 3088
PctI GAATGC 2 cut(s) 41, 2728
PdmI GAANNNNTTC 7 cut(s) 1643, 1847, 2784, 3215, 3415, 3634, 3819
PflFI GACNNNGTC 1 cut(s) 3366
PflMI CCANNNNNTGG 3 cut(s) 463, 1796, 2180
PfoI TCCNGGA 2 cut(s) 1804, 3802
PleI GAGTC 5 cut(s) 109, 198, 1203, 2185, 3523
PmaCI CACGTG 1 cut(s) 2636
PmlI CACGTG 1 cut(s) 2636
PpsI GAGTC 5 cut(s) 109, 198, 1203, 2185, 3523
Ppu21I YACGTR 1 cut(s) 2636
PpuMI RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PshAI GACNNNNGTC 1 cut(s) 471
PsiI TTATAA 2 cut(s) 1304, 2346
Psp124BI GAGCTC 2 cut(s) 1404, 3960
Psp5II RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PspCI CACGTG 1 cut(s) 2636
PspEI GGTNACC 3 cut(s) 2169, 2678, 2807
PspFI CCCAGC 2 cut(s) 2057, 3524
PspOMI GGGCCC 1 cut(s) 2601
PspPPI RGGWCCY 4 cut(s) 907, 1214, 1652, 2495
PspXI VCTCGAGB 1 cut(s) 2019
PsrI GAACNNNNNNTAC 2 cut(s) 1166, 1198
PstNI CAGNNNCTG 1 cut(s) 2180
PsuI RGATCY 5 cut(s) 844, 991, 2062, 3137, 3551
PsyI GACNNNGTC 1 cut(s) 3366
PvuII CAGCTG 3 cut(s) 862, 2057, 2126
RsaI GTAC 5 cut(s) 761, 1116, 2486, 3439, 3736
RsaNI GTAC 5 cut(s) 760, 1115, 2485, 3438, 3735
RseI CAYNNNNRTG 5 cut(s) 1940, 1992, 2463, 3082, 3312
SacI GAGCTC 2 cut(s) 1404, 3960
ScaI AGTACT 2 cut(s) 761, 3736
SchI GAGTC 5 cut(s) 110, 199, 1204, 2186, 3523
SfaNI GCATC 6 cut(s) 2467, 2782, 2998, 3478, 3863, 3908
Sfr274I CTCGAG 1 cut(s) 2019
SfuI TTCGAA 1 cut(s) 224
SinI GGWCC 9 cut(s) 635, 907, 1016, 1214, 1652, 1799, 2495, 2585, 3329
SlaI CTCGAG 1 cut(s) 2019
SmiMI CAYNNNNRTG 5 cut(s) 1940, 1992, 2463, 3082, 3312
SmlI CTYRAG 3 cut(s) 211, 382, 2019
SmoI CTYRAG 3 cut(s) 211, 382, 2019
SphI GCATGC 1 cut(s) 1387
SspI AATATT 4 cut(s) 292, 1558, 2698, 3883
SspMI CTAG 6 cut(s) 522, 599, 989, 1367, 3270, 3719
SstI GAGCTC 2 cut(s) 1404, 3960
StyI CCWWGG 5 cut(s) 252, 2332, 2427, 3067, 3981
TaaI ACNGT 9 cut(s) 1239, 1311, 1363, 1679, 2380, 2678, 3084, 3437, 3610
TaiI ACGT 6 cut(s) 1996, 2223, 2638, 3094, 3370, 3879
TatI WGTACW 4 cut(s) 759, 1114, 3437, 3734
TauI GCSGC 3 cut(s) 496, 3236, 3575
TscAI CASTG 8 cut(s) 801, 814, 1432, 1539, 2200, 2850, 3672, 3769
TseFI GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
Tsp45I GTSAC 6 cut(s) 792, 1030, 1228, 2132, 2169, 3019
TspGWI ACGGA 1 cut(s) 2092
TspRI CASTG 8 cut(s) 801, 814, 1432, 1539, 2200, 2850, 3672, 3769
Tth111I GACNNNGTC 1 cut(s) 3366
Van91I CCANNNNNTGG 3 cut(s) 463, 1796, 2180
Vha464I CTTAAG 1 cut(s) 382
VpaK11BI GGWCC 9 cut(s) 635, 907, 1016, 1214, 1652, 1799, 2495, 2585, 3329
XapI RAATTY 4 cut(s) 279, 1147, 2441, 3355
XbaI TCTAGA 1 cut(s) 598
XceI RCATGY 3 cut(s) 680, 1387, 1945
XcmI CCANNNNNNNNNTGG 2 cut(s) 2595, 4013
XhoI CTCGAG 1 cut(s) 2019
XmiI GTMKAC 1 cut(s) 3088
XmnI GAANNNNTTC 7 cut(s) 1643, 1847, 2784, 3215, 3415, 3634, 3819
XspI CTAG 6 cut(s) 522, 599, 989, 1367, 3270, 3719
ZrmI AGTACT 2 cut(s) 761, 3736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.