Rmu_sc0001423.1_g000017

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001423.1
OG0015637
Physical Location & Seq
Reverse (-)
92777 .. 95588
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
atgaaggtgacaagagccgtgacagtcaacataaaacccagtttaggcaccaagataacttacaaggtggaagctaacactgcagatgtaggttttctcatcaaagagaaacaacatttcgtatacgagaggcaaaggaatgatctcatagtccaccaggtgataacaccgatggaggcgctcaccggcgataggactcttaatttgaccatcaatagcggatccagaatccgaatatgtgatcacctctacaaaatttcagccaaattggtgatcgttcagttacatacctatacttgggggtttttcctcttgattttggattgggctgaccaccctggacggaaggaagctcttggtcccattaccgatgatatcatgagagctcgtagggatgaaatggttgtaccaggtgagggaatgccaatctcaagccaacgtggggtgaaagaaaacttgaggatccagttcaatgtcaagtttcctacgagcagcctcactgttgaacagaaattggatttgaaaagaggcttgggaggagcttcattttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ_C

Protein Analysis

183

Amino Acids

20.7

Weight (kDa)

9.64

Isoelectric Point (pI)

16.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 6 - 79 2.9e-06 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccI GTMKAC 1 cut(s) 123
AciI CCGC 1 cut(s) 219
AclWI GGATC 4 cut(s) 216, 229, 457, 470
AcsI RAATTY 1 cut(s) 255
AdeI CACNNNGTG 1 cut(s) 160
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 5 cut(s) 44, 192, 297, 416, 442
AgsI TTSAA 3 cut(s) 472, 506, 523
AjnI CCWGG 3 cut(s) 156, 337, 409
AjuI GAANNNNNNNTTGG 2 cut(s) 515, 547
AluBI AGCT 4 cut(s) 74, 353, 386, 542
AluI AGCT 4 cut(s) 74, 353, 386, 542
Alw21I GWGCWC 1 cut(s) 388
AlwI GGATC 4 cut(s) 216, 229, 457, 470
ApeKI GCWGC 1 cut(s) 492
ApoI RAATTY 1 cut(s) 255
ArsI GACNNNNNNTTYG 2 cut(s) 187, 219
AspLEI GCGC 1 cut(s) 181
AspS9I GGNCC 1 cut(s) 359
AsuHPI GGTGA 7 cut(s) 19, 172, 175, 236, 283, 425, 457
AvaII GGWCC 1 cut(s) 359
BamHI GGATCC 2 cut(s) 221, 462
BanI GGYRCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 388
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 1 cut(s) 504
BccI CCATC 2 cut(s) 166, 218
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 3 cut(s) 158, 339, 411
BclI TGATCA 1 cut(s) 241
BfmI CTRYAG 1 cut(s) 81
BfoI RGCGCY 1 cut(s) 182
BisI GCNGC 1 cut(s) 493
BlsI GCNGC 1 cut(s) 494
Bme1390I CCNGG 3 cut(s) 158, 339, 411
Bme18I GGWCC 1 cut(s) 359
BmgT120I GGNCC 1 cut(s) 359
BmiI GGNNCC 4 cut(s) 49, 223, 361, 464
BmrFI CCNGG 3 cut(s) 158, 339, 411
BmrI ACTGGG 1 cut(s) 33
BmuI ACTGGG 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 415, 478
BsaJI CCNNGG 1 cut(s) 337
Bsc4I CCNNNNNNNGG 5 cut(s) 44, 192, 297, 416, 442
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 39, 466
BseBI CCWGG 3 cut(s) 158, 339, 411
BseDI CCNNGG 1 cut(s) 337
BseGI GGATG 1 cut(s) 400
BseLI CCNNNNNNNGG 5 cut(s) 44, 192, 297, 416, 442
BseNI ACTGG 2 cut(s) 39, 466
BseRI GAGGAG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 504
BshNI GGYRCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 388
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 345
BslI CCNNNNNNNGG 5 cut(s) 44, 192, 297, 416, 442
BsmFI GGGAC 1 cut(s) 345
BsmI GAATGC 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 388
Bsp143I GATC 5 cut(s) 142, 221, 241, 273, 462
BspACI CCGC 1 cut(s) 219
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 4 cut(s) 49, 223, 361, 464
BspMAI CTGCAG 1 cut(s) 85
BspPI GGATC 4 cut(s) 216, 229, 457, 470
BspT107I GGYRCC 1 cut(s) 47
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 39, 466
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 337
BssMI GATC 5 cut(s) 142, 221, 241, 273, 462
BssNAI GTATAC 1 cut(s) 124
Bst1107I GTATAC 1 cut(s) 124
Bst2UI CCWGG 3 cut(s) 158, 339, 411
Bst4CI ACNGT 2 cut(s) 25, 502
BstF5I GGATG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 182
BstHHI GCGC 1 cut(s) 181
BstKTI GATC 5 cut(s) 145, 224, 244, 276, 465
BstMBI GATC 5 cut(s) 142, 221, 241, 273, 462
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNI CCWGG 3 cut(s) 158, 339, 411
BstSCI CCNGG 3 cut(s) 156, 337, 409
BstSFI CTRYAG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 504
BstX2I RGATCY 2 cut(s) 221, 462
BstYI RGATCY 2 cut(s) 221, 462
BstZ17I GTATAC 1 cut(s) 124
BtsCI GGATG 1 cut(s) 400
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 2 cut(s) 78, 498
CciI TCATGA 1 cut(s) 378
CfoI GCGC 1 cut(s) 181
Cfr10I RCCGGY 1 cut(s) 185
Cfr13I GGNCC 1 cut(s) 359
CsiI ACCWGGT 2 cut(s) 156, 409
Csp6I GTAC 1 cut(s) 407
CviAII CATG 1 cut(s) 379
CviQI GTAC 1 cut(s) 407
DpnI GATC 5 cut(s) 144, 223, 243, 275, 464
DpnII GATC 5 cut(s) 142, 221, 241, 273, 462
DraIII CACNNNGTG 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 386
Eco24I GRGCYC 1 cut(s) 388
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 359
Eco53kI GAGCTC 1 cut(s) 386
EcoICRI GAGCTC 1 cut(s) 386
EcoRII CCWGG 3 cut(s) 156, 337, 409
EcoRV GATATC 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 388
FaeI CATG 1 cut(s) 382
FaiI YATR 7 cut(s) 32, 124, 149, 238, 288, 294, 380
FaqI GGGAC 1 cut(s) 345
FatI CATG 1 cut(s) 378
FbaI TGATCA 1 cut(s) 241
FblI GTMKAC 1 cut(s) 123
Fnu4HI GCNGC 1 cut(s) 493
FokI GGATG 1 cut(s) 407
FriOI GRGCYC 1 cut(s) 388
Fsp4HI GCNGC 1 cut(s) 493
GlaI GCGC 1 cut(s) 180
GluI GCNGC 1 cut(s) 493
HaeII RGCGCY 1 cut(s) 182
HapII CCGG 1 cut(s) 186
HhaI GCGC 1 cut(s) 181
Hin1II CATG 1 cut(s) 382
Hin6I GCGC 1 cut(s) 179
HinP1I GCGC 1 cut(s) 179
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 2 cut(s) 196, 228
HpaII CCGG 1 cut(s) 186
HphI GGTGA 7 cut(s) 19, 172, 175, 236, 283, 425, 457
Hpy166II GTNNAC 3 cut(s) 28, 124, 154
Hpy188I TCNGA 1 cut(s) 233
Hpy188III TCNNGA 3 cut(s) 225, 313, 379
Hpy8I GTNNAC 3 cut(s) 28, 124, 154
HpyAV CCTTC 1 cut(s) 340
HpyCH4III ACNGT 2 cut(s) 25, 502
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 1 cut(s) 83
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpySE526I ACGT 1 cut(s) 439
Hsp92II CATG 1 cut(s) 382
HspAI GCGC 1 cut(s) 179
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 5 cut(s) 142, 221, 241, 273, 462
LmnI GCTCC 1 cut(s) 539
Lsp1109I GCAGC 1 cut(s) 504
MabI ACCWGGT 2 cut(s) 156, 409
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 3 cut(s) 7, 19, 282
MalI GATC 5 cut(s) 144, 223, 243, 275, 464
MboI GATC 5 cut(s) 142, 221, 241, 273, 462
MflI RGATCY 2 cut(s) 221, 462
MhlI GDGCHC 1 cut(s) 388
MluCI AATT 4 cut(s) 202, 255, 266, 512
MlyI GAGTC 1 cut(s) 190
MnlI CCTC 9 cut(s) 123, 169, 257, 320, 409, 453, 506, 521, 530
MseI TTAA 1 cut(s) 201
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 3 cut(s) 158, 339, 411
Mva1269I GAATGC 1 cut(s) 426
MvaI CCWGG 3 cut(s) 158, 339, 411
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 5 cut(s) 142, 221, 241, 273, 462
NlaIII CATG 1 cut(s) 382
NlaIV GGNNCC 4 cut(s) 49, 223, 361, 464
NmuCI GTSAC 2 cut(s) 7, 19
PagI TCATGA 1 cut(s) 378
PctI GAATGC 1 cut(s) 426
PfeI GAWTC 1 cut(s) 228
PkrI GCNGC 1 cut(s) 494
PleI GAGTC 1 cut(s) 190
PpsI GAGTC 1 cut(s) 190
Psp124BI GAGCTC 1 cut(s) 388
Psp6I CCWGG 3 cut(s) 156, 337, 409
PspGI CCWGG 3 cut(s) 156, 337, 409
PspN4I GGNNCC 4 cut(s) 49, 223, 361, 464
PspPI GGNCC 1 cut(s) 359
PstI CTGCAG 1 cut(s) 85
PsuI RGATCY 2 cut(s) 221, 462
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 1 cut(s) 493
Sau3AI GATC 5 cut(s) 142, 221, 241, 273, 462
Sau96I GGNCC 1 cut(s) 359
SchI GAGTC 1 cut(s) 190
ScrFI CCNGG 3 cut(s) 158, 339, 411
SduI GDGCHC 1 cut(s) 388
SexAI ACCWGGT 2 cut(s) 156, 409
SfcI CTRYAG 1 cut(s) 81
SgrAI CRCCGGYG 1 cut(s) 185
SinI GGWCC 1 cut(s) 359
SmlI CTYRAG 2 cut(s) 430, 457
SmoI CTYRAG 2 cut(s) 430, 457
Sse9I AATT 4 cut(s) 202, 255, 266, 512
SsiI CCGC 1 cut(s) 219
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 3 cut(s) 156, 337, 409
TaaI ACNGT 2 cut(s) 25, 502
TaiI ACGT 1 cut(s) 442
TasI AATT 4 cut(s) 202, 255, 266, 512
TfiI GAWTC 1 cut(s) 228
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 2 cut(s) 85, 505
TseFI GTSAC 2 cut(s) 7, 19
TseI GCWGC 1 cut(s) 492
Tsp45I GTSAC 2 cut(s) 7, 19
TspDTI ATGAA 3 cut(s) 17, 411, 534
TspGWI ACGGA 1 cut(s) 358
TspRI CASTG 2 cut(s) 85, 505
VpaK11BI GGWCC 1 cut(s) 359
XapI RAATTY 1 cut(s) 255
XmiI GTMKAC 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.