Rroxscaffold_3G00232700

DnaJ homolog subfamily B member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0015637
Physical Location & Seq
Reverse (-)
18353740 .. 18354267
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232700.1

Sequence Viewer

Length: 528 bp
ATGAAGGTGACGAGAGCCGTGACGAATGCTTTTGGGAAGGAGACTATGGTGGAGCAGCCTTTGACAGTCGACATAAAACCTGGTTTCCGGCACGGCGCCAAGATAACTTACAAGGTGGAAGCTAATACGGCAGATTTAGGTTTTGTCATCAAAGAGAAACAACATTCCGTGTACGAGAGGCATGGGAATGATCTCAAAGTCCACCAGGAGATAACAATGCTGGAGGCGCTAACCGGCGGTAGGACTCTTGATCTGACCACCCTGGACGGAAGGAAGCTCATGGTCCCGTTACCCGATGATGTCATCAGAGCTCCTGGAGATGAAATGGTTGTACCAGGTGAGGGAATGCCAATCTCAAGCCAACCTGGGGTTAAAGGAAACTTGAGGATCAAGTTCAATGTCAAGTTTCCTTCGAGCAGCCTCACTGCAGAACAGAAATTGGATTTGAAAAGAGGCTTGGGAGGAGCTTCTTTTTACTACGCACATGCAATATTTGGTTACTTCTTTTTTATTTGCATTTGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ_C

Protein Analysis

175

Amino Acids

19.35

Weight (kDa)

8.83

Isoelectric Point (pI)

25.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ_C PF01556 16 - 137 1.3e-23 DnaJ C terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 95
AccI GTMKAC 1 cut(s) 69
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 395
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 2 cut(s) 173, 333
AfiI CCNNNNNNNGG 3 cut(s) 240, 341, 367
AgsI TTSAA 2 cut(s) 397, 448
AjnI CCWGG 6 cut(s) 79, 204, 261, 313, 334, 364
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 4 cut(s) 122, 277, 311, 467
AluI AGCT 4 cut(s) 122, 277, 311, 467
Alw21I GWGCWC 1 cut(s) 313
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 1 cut(s) 395
ApeKI GCWGC 2 cut(s) 55, 417
AspLEI GCGC 2 cut(s) 98, 229
AspS9I GGNCC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 19, 350
AvaII GGWCC 1 cut(s) 283
BanI GGYRCC 1 cut(s) 95
BanII GRGCYC 1 cut(s) 313
Bbv12I GWGCWC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 67, 429
BceAI ACGGC 3 cut(s) 2, 109, 144
BciT130I CCWGG 6 cut(s) 81, 206, 263, 315, 336, 366
BcoDI GTCTC 1 cut(s) 35
BfmI CTRYAG 1 cut(s) 426
BfoI RGCGCY 2 cut(s) 99, 230
BisI GCNGC 2 cut(s) 56, 418
BlsI GCNGC 2 cut(s) 57, 419
Bme1390I CCNGG 6 cut(s) 81, 206, 263, 315, 336, 366
Bme18I GGWCC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 283
BmiI GGNNCC 2 cut(s) 97, 285
BmrFI CCNGG 6 cut(s) 81, 206, 263, 315, 336, 366
BpmI CTGGAG 2 cut(s) 242, 336
BpuEI CTTGAG 2 cut(s) 340, 403
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 2 cut(s) 261, 365
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 341, 367
Bse118I RCCGGY 1 cut(s) 233
BseBI CCWGG 6 cut(s) 81, 206, 263, 315, 336, 366
BseDI CCNNGG 2 cut(s) 261, 365
BseLI CCNNNNNNNGG 3 cut(s) 240, 341, 367
BseRI GAGGAG 1 cut(s) 477
BseXI GCAGC 2 cut(s) 67, 429
BshNI GGYRCC 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 313
BsiSI CCGG 2 cut(s) 88, 234
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 3 cut(s) 240, 341, 367
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 269
BsmI GAATGC 2 cut(s) 31, 351
Bsp1286I GDGCHC 1 cut(s) 313
Bsp143I GATC 3 cut(s) 190, 250, 387
BspACI CCGC 1 cut(s) 237
BspLI GGNNCC 2 cut(s) 97, 285
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 95
BsrFI RCCGGY 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 233
BssECI CCNNGG 2 cut(s) 261, 365
BssMI GATC 3 cut(s) 190, 250, 387
BssNI GRCGYC 1 cut(s) 96
Bst2UI CCWGG 6 cut(s) 81, 206, 263, 315, 336, 366
Bst4CI ACNGT 1 cut(s) 67
BstACI GRCGYC 1 cut(s) 96
BstH2I RGCGCY 2 cut(s) 99, 230
BstHHI GCGC 2 cut(s) 98, 229
BstKTI GATC 3 cut(s) 193, 253, 390
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 3 cut(s) 190, 250, 387
BstMWI GCNNNNNNNGC 2 cut(s) 128, 226
BstNI CCWGG 6 cut(s) 81, 206, 263, 315, 336, 366
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 6 cut(s) 79, 204, 261, 313, 334, 364
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 2 cut(s) 67, 429
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 423
CfoI GCGC 2 cut(s) 98, 229
Cfr10I RCCGGY 1 cut(s) 233
Cfr13I GGNCC 1 cut(s) 283
CsiI ACCWGGT 2 cut(s) 79, 334
Csp6I GTAC 2 cut(s) 172, 332
CviAII CATG 3 cut(s) 182, 280, 485
CviJI RGCY 9 cut(s) 17, 58, 122, 277, 311, 360, 420, 456, 467
CviKI_1 RGCY 9 cut(s) 17, 58, 122, 277, 311, 360, 420, 456, 467
CviQI GTAC 2 cut(s) 172, 332
DinI GGCGCC 1 cut(s) 97
DpnI GATC 3 cut(s) 192, 252, 389
DpnII GATC 3 cut(s) 190, 250, 387
Ecl136II GAGCTC 1 cut(s) 311
Eco24I GRGCYC 1 cut(s) 313
Eco47I GGWCC 1 cut(s) 283
Eco53kI GAGCTC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 311
EcoRII CCWGG 6 cut(s) 79, 204, 261, 313, 334, 364
EcoT38I GRGCYC 1 cut(s) 313
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
FaeI CATG 3 cut(s) 185, 283, 488
FaiI YATR 6 cut(s) 47, 74, 183, 281, 486, 526
FaqI GGGAC 1 cut(s) 269
FatI CATG 3 cut(s) 181, 279, 484
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 2 cut(s) 56, 418
FriOI GRGCYC 1 cut(s) 313
Fsp4HI GCNGC 2 cut(s) 56, 418
GlaI GCGC 2 cut(s) 97, 228
GluI GCNGC 2 cut(s) 56, 418
GsuI CTGGAG 2 cut(s) 242, 336
HaeII RGCGCY 2 cut(s) 99, 230
HapII CCGG 2 cut(s) 88, 234
HhaI GCGC 2 cut(s) 98, 229
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 3 cut(s) 185, 283, 488
Hin6I GCGC 2 cut(s) 96, 227
HinP1I GCGC 2 cut(s) 96, 227
HincII GTYRAC 1 cut(s) 70
HindII GTYRAC 1 cut(s) 70
HinfI GANTC 1 cut(s) 244
HpaII CCGG 2 cut(s) 88, 234
HphI GGTGA 2 cut(s) 19, 350
Hpy166II GTNNAC 3 cut(s) 70, 172, 202
Hpy188I TCNGA 2 cut(s) 255, 308
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 3 cut(s) 70, 172, 202
HpyAV CCTTC 3 cut(s) 31, 264, 420
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4V TGCA 3 cut(s) 428, 488, 516
HpyF10VI GCNNNNNNNGC 2 cut(s) 128, 226
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 3 cut(s) 185, 283, 488
HspAI GCGC 2 cut(s) 96, 227
KasI GGCGCC 1 cut(s) 95
Kzo9I GATC 3 cut(s) 190, 250, 387
LmnI GCTCC 3 cut(s) 52, 316, 464
Lsp1109I GCAGC 2 cut(s) 67, 429
MabI ACCWGGT 2 cut(s) 79, 334
MaeIII GTNAC 4 cut(s) 7, 19, 288, 497
MalI GATC 3 cut(s) 192, 252, 389
MboI GATC 3 cut(s) 190, 250, 387
MhlI GDGCHC 1 cut(s) 313
MluCI AATT 1 cut(s) 437
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 1 cut(s) 238
MnlI CCTC 7 cut(s) 171, 217, 334, 378, 431, 446, 455
MseI TTAA 1 cut(s) 372
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 2 cut(s) 88, 234
MspR9I CCNGG 6 cut(s) 81, 206, 263, 315, 336, 366
Mva1269I GAATGC 2 cut(s) 31, 351
MvaI CCWGG 6 cut(s) 81, 206, 263, 315, 336, 366
MwoI GCNNNNNNNGC 2 cut(s) 128, 226
NarI GGCGCC 1 cut(s) 96
NdeII GATC 3 cut(s) 190, 250, 387
NlaIII CATG 3 cut(s) 185, 283, 488
NlaIV GGNNCC 2 cut(s) 97, 285
NmuCI GTSAC 2 cut(s) 7, 19
NspI RCATGY 1 cut(s) 488
PctI GAATGC 2 cut(s) 31, 351
PfoI TCCNGGA 1 cut(s) 313
PkrI GCNGC 2 cut(s) 57, 419
PleI GAGTC 1 cut(s) 238
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 1 cut(s) 238
Psp124BI GAGCTC 1 cut(s) 313
Psp6I CCWGG 6 cut(s) 79, 204, 261, 313, 334, 364
PspGI CCWGG 6 cut(s) 79, 204, 261, 313, 334, 364
PspN4I GGNNCC 2 cut(s) 97, 285
PspPI GGNCC 1 cut(s) 283
PstI CTGCAG 1 cut(s) 430
RsaI GTAC 2 cut(s) 173, 333
RsaNI GTAC 2 cut(s) 172, 332
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 313
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 1 cut(s) 372
SatI GCNGC 2 cut(s) 56, 418
Sau3AI GATC 3 cut(s) 190, 250, 387
Sau96I GGNCC 1 cut(s) 283
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 6 cut(s) 81, 206, 263, 315, 336, 366
SduI GDGCHC 1 cut(s) 313
SexAI ACCWGGT 2 cut(s) 79, 334
SfcI CTRYAG 1 cut(s) 426
SfoI GGCGCC 1 cut(s) 97
SinI GGWCC 1 cut(s) 283
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 355, 382
SmoI CTYRAG 2 cut(s) 355, 382
Sse9I AATT 1 cut(s) 437
SsiI CCGC 1 cut(s) 237
SspDI GGCGCC 1 cut(s) 95
SspI AATATT 1 cut(s) 492
SstI GAGCTC 1 cut(s) 313
StyD4I CCNGG 6 cut(s) 79, 204, 261, 313, 334, 364
TaaI ACNGT 1 cut(s) 67
TaqI TCGA 2 cut(s) 69, 413
TasI AATT 1 cut(s) 437
Tru1I TTAA 1 cut(s) 372
Tru9I TTAA 1 cut(s) 372
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 2 cut(s) 7, 19
TseI GCWGC 2 cut(s) 55, 417
Tsp45I GTSAC 2 cut(s) 7, 19
TspDTI ATGAA 2 cut(s) 17, 336
TspGWI ACGGA 2 cut(s) 157, 282
TspRI CASTG 1 cut(s) 430
VpaK11BI GGWCC 1 cut(s) 283
XceI RCATGY 1 cut(s) 488
XmiI GTMKAC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.