Rmu_sc0001630.1_g000028

Bacterial trigger factor protein (TF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001630.1
OG0011553
Physical Location & Seq
Reverse (-)
215609 .. 218822
3214 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 630 bp
atggcgtcgttgacgacgatgataacagttccgtcgaattgcccctcccttggacttcccatgcacttttctgtgagaaaacactgcacttgtgtcgttgcttatcaaaatgccgccacaaaaatggggtcatatagctctcaacataatcttttcactcgtgtgcatgagattggtattgctcgcttatccaggccgatattcgcagccggttcaggtttagaagcatccattgcggaagaaaatgtgataaagttgaaaaccacaaagattgttgtcgagtcccaagatgagaatagtatacaagttagagtggacgtggctggggatgaaacgcaaaaagtttttgatcaggttttgacaaatttggcacgcacagctccaccagttccaaaaagctttctcttggatatacttggtaaagagcgtgtcacaaagtttgtcatacaagaattagtaagctctgctatggctgattatgtaaagcaggaaaatctgactgtgaaggaaaacaagatcaacaccacccaatcggcagaagaactccaatcgttgttcactccggggaatgaatttgcatttaatgctatagttgaacttgaaaagtcagaaattgaaaagccaacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Trigger_N

Protein Analysis

209

Amino Acids

22.99

Weight (kDa)

5.85

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Trigger_N PF05697 90 - 200 2.9e-06 Bacterial trigger factor protein (TF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 301
AciI CCGC 2 cut(s) 114, 236
AcsI RAATTY 2 cut(s) 364, 572
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 4 cut(s) 259, 596, 602, 617
AjiI CACGTC 1 cut(s) 319
AjnI CCWGG 1 cut(s) 191
AleI CACNNNNGTG 1 cut(s) 161
AluBI AGCT 4 cut(s) 138, 380, 399, 462
AluI AGCT 4 cut(s) 138, 380, 399, 462
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 206
ApoI RAATTY 2 cut(s) 364, 572
ArsI GACNNNNNNTTYG 2 cut(s) 113, 145
AsuC2I CCSGG 1 cut(s) 564
BauI CACGAG 1 cut(s) 159
BbvI GCAGC 1 cut(s) 218
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 193
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 1 cut(s) 564
BfmI CTRYAG 1 cut(s) 588
BisI GCNGC 2 cut(s) 114, 207
BlsI GCNGC 2 cut(s) 115, 208
Bme1390I CCNGG 2 cut(s) 193, 564
BmgBI CACGTC 1 cut(s) 319
BmrFI CCNGG 2 cut(s) 193, 564
BmsI GCATC 1 cut(s) 236
BpuMI CCSGG 1 cut(s) 564
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 2 cut(s) 49, 563
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 1 cut(s) 386
Bse3DI GCAATG 1 cut(s) 231
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 49, 563
BseGI GGATG 2 cut(s) 227, 334
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMI GCAATG 1 cut(s) 231
BseNI ACTGG 1 cut(s) 386
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 70
BshFI GGCC 1 cut(s) 196
BsiSI CCGG 2 cut(s) 210, 563
BslFI GGGAC 1 cut(s) 268
BslI CCNNNNNNNGG 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 268
BsnI GGCC 1 cut(s) 196
Bsp143I GATC 2 cut(s) 349, 516
BspACI CCGC 2 cut(s) 114, 236
BspANI GGCC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 231
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 1 cut(s) 386
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 2 cut(s) 49, 563
BssMI GATC 2 cut(s) 349, 516
BssNAI GTATAC 1 cut(s) 302
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 49
Bst1107I GTATAC 1 cut(s) 302
Bst2BI CACGAG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 2 cut(s) 28, 502
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 2 cut(s) 233, 584
BstC8I GCNNGC 2 cut(s) 184, 373
BstF5I GGATG 2 cut(s) 227, 334
BstKTI GATC 2 cut(s) 352, 519
BstMBI GATC 2 cut(s) 349, 516
BstMWI GCNNNNNNNGC 3 cut(s) 233, 377, 584
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 191, 562
BstSFI CTRYAG 1 cut(s) 588
BstV1I GCAGC 1 cut(s) 218
BstXI CCANNNNNNTGG 1 cut(s) 124
BstZ17I GTATAC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 196
BtrI CACGTC 1 cut(s) 319
BtsCI GGATG 2 cut(s) 227, 334
BtsI GCAGTG 1 cut(s) 82
BtsIMutI CAGTG 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 184, 373
Cfr10I RCCGGY 1 cut(s) 209
CspCI CAANNNNNGTGG 2 cut(s) 253, 288
CviAII CATG 2 cut(s) 61, 167
CviJI RGCY 9 cut(s) 138, 196, 209, 323, 380, 399, 462, 473, 622
CviKI_1 RGCY 9 cut(s) 138, 196, 209, 323, 380, 399, 462, 473, 622
DpnI GATC 2 cut(s) 351, 518
DpnII GATC 2 cut(s) 349, 516
Eco130I CCWWGG 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 2 cut(s) 64, 170
FaqI GGGAC 1 cut(s) 268
FatI CATG 2 cut(s) 60, 166
FbaI TGATCA 1 cut(s) 349
FblI GTMKAC 1 cut(s) 301
Fnu4HI GCNGC 2 cut(s) 114, 207
FokI GGATG 2 cut(s) 214, 341
Fsp4HI GCNGC 2 cut(s) 114, 207
GluI GCNGC 2 cut(s) 114, 207
GsaI CCCAGC 1 cut(s) 327
HaeIII GGCC 1 cut(s) 196
HapII CCGG 2 cut(s) 210, 563
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 64, 170
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 397
HinfI GANTC 1 cut(s) 281
HpaII CCGG 2 cut(s) 210, 563
Hpy166II GTNNAC 4 cut(s) 12, 302, 316, 558
Hpy188I TCNGA 2 cut(s) 498, 610
Hpy8I GTNNAC 4 cut(s) 12, 302, 316, 558
Hpy99I CGWCG 3 cut(s) 10, 19, 37
HpyAV CCTTC 1 cut(s) 499
HpyCH4III ACNGT 2 cut(s) 28, 502
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 4 cut(s) 64, 87, 166, 578
HpyF10VI GCNNNNNNNGC 3 cut(s) 233, 377, 584
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 64, 170
Ksp22I TGATCA 1 cut(s) 349
Kzo9I GATC 2 cut(s) 349, 516
LmnI GCTCC 1 cut(s) 385
LpnPI CCDG 9 cut(s) 178, 201, 205, 223, 309, 338, 399, 473, 576
Lsp1109I GCAGC 1 cut(s) 218
LweI GCATC 1 cut(s) 236
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 430
MalI GATC 2 cut(s) 351, 518
MboI GATC 2 cut(s) 349, 516
MboII GAAGA 2 cut(s) 251, 551
MluCI AATT 5 cut(s) 37, 364, 452, 572, 612
MlyI GAGTC 1 cut(s) 290
MnlI CCTC 1 cut(s) 55
MseI TTAA 1 cut(s) 582
MslI CAYNNNNRTG 2 cut(s) 122, 161
MspI CCGG 2 cut(s) 210, 563
MspR9I CCNGG 2 cut(s) 193, 564
MvaI CCWGG 1 cut(s) 193
MwoI GCNNNNNNNGC 3 cut(s) 233, 377, 584
NciI CCSGG 1 cut(s) 564
NdeII GATC 2 cut(s) 349, 516
NlaIII CATG 2 cut(s) 64, 170
NmuCI GTSAC 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PkrI GCNGC 2 cut(s) 115, 208
PleI GAGTC 1 cut(s) 289
PpsI GAGTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 1 cut(s) 191
RseI CAYNNNNRTG 2 cut(s) 122, 161
SaqAI TTAA 1 cut(s) 582
SatI GCNGC 2 cut(s) 114, 207
Sau3AI GATC 2 cut(s) 349, 516
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 2 cut(s) 193, 564
SetI ASST 8 cut(s) 140, 220, 321, 357, 382, 401, 464, 629
SfaNI GCATC 1 cut(s) 236
SfcI CTRYAG 1 cut(s) 588
SmiMI CAYNNNNRTG 2 cut(s) 122, 161
Sse9I AATT 5 cut(s) 37, 364, 452, 572, 612
SsiI CCGC 2 cut(s) 114, 236
StyD4I CCNGG 2 cut(s) 191, 562
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 2 cut(s) 28, 502
TaiI ACGT 1 cut(s) 321
TaqI TCGA 2 cut(s) 35, 279
TasI AATT 5 cut(s) 37, 364, 452, 572, 612
TauI GCSGC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 582
Tru9I TTAA 1 cut(s) 582
TscAI CASTG 1 cut(s) 89
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 206
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 345, 585
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 89
XapI RAATTY 2 cut(s) 364, 572
XmiI GTMKAC 1 cut(s) 301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.