Rmu_sc0001929.1_g000002

NifU-like protein 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001929.1
OG0015718
Physical Location & Seq
Reverse (-)
4023 .. 6638
2616 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 723 bp
atggttccttcttatttcttctggttgcccaaaaccccaattcattcttctcaaatcccagtgaaatcccatcaatccccacaacttatgcttgtaaaacggtgctgtctaactcctcaaagaagaatctttctctgttccgccattagagcttcgaacgcaagcgttgcgtccgagtcttcttcttcttctccgggactctactcggccaagcaatttgagctcatagttcccaacattgacttggttctcgaggacgtcggcccctatctcatcaacgatggtggaaacatcgacgttgtctcaacaaccaccatgaaagtggggattgaacgagtgcttaacgaaaagtttggagatgcactcaaggatattcaacaagtctttgatgaagaaaatagagagaccacagttgagagcagtggagattttggggctaatgtgaagcgccttgaagtggaaactgaggaaaagatcaatcacctctcaacagagggctcaaggatatcaggtgatgttgtccagatgcttttaaagcatgtgacgacgacaattgtctcatctctttgctcaaccagcaaagcttccctcttatttacacacgtcaaattccctgactacaaatcaggcaaggcaacatctgaaactggttcacctgtggatgaaactgaatttgagaagttagaagagaaagcctttaatttgagaaaggtattcatttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NifU

Protein Analysis

240

Amino Acids

26.58

Weight (kDa)

5.62

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V-ATPase_G PF03179 129 - 183 7.6e-06 Vacuolar (H+)-ATPase G subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AciI CCGC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 207
AcsI RAATTY 2 cut(s) 608, 671
AcyI GRCGYC 1 cut(s) 258
AfiI CCNNNNNNNGG 1 cut(s) 457
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 3 cut(s) 332, 377, 455
AjiI CACGTC 1 cut(s) 604
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 3 cut(s) 152, 223, 584
AluI AGCT 3 cut(s) 152, 223, 584
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 3 cut(s) 307, 398, 562
Ama87I CYCGRG 1 cut(s) 251
AoxI GGCC 2 cut(s) 207, 262
ApoI RAATTY 2 cut(s) 608, 671
AspLEI GCGC 1 cut(s) 450
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 473, 524, 645
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 251
BanII GRGCYC 2 cut(s) 225, 500
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 1 cut(s) 225
BccI CCATC 2 cut(s) 78, 275
BcnI CCSGG 1 cut(s) 195
BcoDI GTCTC 3 cut(s) 307, 398, 562
BfoI RGCGCY 1 cut(s) 451
Bme1390I CCNGG 1 cut(s) 195
BmeT110I CYCGRG 1 cut(s) 251
BmgBI CACGTC 1 cut(s) 604
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 2 cut(s) 6, 265
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 1 cut(s) 53
BmsI GCATC 2 cut(s) 349, 516
BmuI ACTGGG 1 cut(s) 53
BoxI GACNNNNGTC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 348, 380
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 2 cut(s) 350, 484
BpuMI CCSGG 1 cut(s) 195
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 2 cut(s) 59, 652
BseGI GGATG 1 cut(s) 667
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 2 cut(s) 59, 652
BseRI GAGGAG 1 cut(s) 105
BshFI GGCC 2 cut(s) 209, 264
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 1 cut(s) 194
BslFI GGGAC 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 3 cut(s) 307, 398, 562
BsmFI GGGAC 1 cut(s) 210
BsnI GGCC 2 cut(s) 209, 264
Bso31I GGTCTC 1 cut(s) 398
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 225, 500
Bsp143I GATC 1 cut(s) 474
BspACI CCGC 1 cut(s) 141
BspANI GGCC 2 cut(s) 209, 264
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 2 cut(s) 6, 265
BspT104I TTCGAA 1 cut(s) 155
BspTNI GGTCTC 1 cut(s) 398
BsrI ACTGG 2 cut(s) 59, 652
BssMI GATC 1 cut(s) 474
BssNI GRCGYC 1 cut(s) 258
Bst4CI ACNGT 2 cut(s) 102, 412
Bst6I CTCTTC 1 cut(s) 681
BstACI GRCGYC 1 cut(s) 258
BstAPI GCANNNNNTGC 1 cut(s) 167
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 1 cut(s) 667
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 1 cut(s) 450
BstKTI GATC 1 cut(s) 477
BstMAI GTCTC 3 cut(s) 307, 398, 562
BstMBI GATC 1 cut(s) 474
BstMWI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 535, 576
BstNSI RCATGY 1 cut(s) 542
BstPAI GACNNNNGTC 1 cut(s) 554
BstSCI CCNGG 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 171
BstXI CCANNNNNNTGG 1 cut(s) 322
BsuRI GGCC 2 cut(s) 209, 264
BtrI CACGTC 1 cut(s) 604
BtsCI GGATG 1 cut(s) 667
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 2 cut(s) 66, 427
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 263
CseI GACGC 1 cut(s) 159
CspCI CAANNNNNGTGG 2 cut(s) 265, 300
CviAII CATG 2 cut(s) 316, 539
CviJI RGCY 8 cut(s) 152, 209, 223, 264, 437, 498, 584, 695
CviKI_1 RGCY 8 cut(s) 152, 209, 223, 264, 437, 498, 584, 695
DdeI CTNAG 1 cut(s) 465
DpnI GATC 1 cut(s) 476
DpnII GATC 1 cut(s) 474
DraI TTTAAA 1 cut(s) 534
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 681
EarI CTCTTC 1 cut(s) 681
EciI GGCGGA 1 cut(s) 130
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 2 cut(s) 225, 500
Eco31I GGTCTC 1 cut(s) 398
Eco32I GATATC 1 cut(s) 507
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 223
EcoRV GATATC 1 cut(s) 507
EcoT38I GRGCYC 2 cut(s) 225, 500
FaeI CATG 2 cut(s) 319, 542
FaiI YATR 4 cut(s) 89, 227, 317, 540
FaqI GGGAC 1 cut(s) 210
FatI CATG 2 cut(s) 315, 538
FokI GGATG 1 cut(s) 674
FriOI GRGCYC 2 cut(s) 225, 500
GlaI GCGC 1 cut(s) 449
HaeII RGCGCY 1 cut(s) 451
HaeIII GGCC 2 cut(s) 209, 264
HapII CCGG 1 cut(s) 194
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 450
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 319, 542
Hin6I GCGC 1 cut(s) 448
HinP1I GCGC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 3 cut(s) 126, 176, 198
HpaII CCGG 1 cut(s) 194
HphI GGTGA 3 cut(s) 473, 524, 645
Hpy166II GTNNAC 1 cut(s) 653
Hpy188I TCNGA 2 cut(s) 175, 643
Hpy188III TCNNGA 2 cut(s) 251, 523
Hpy8I GTNNAC 1 cut(s) 653
Hpy99I CGWCG 3 cut(s) 263, 299, 550
HpyAV CCTTC 1 cut(s) 18
HpyCH4III ACNGT 2 cut(s) 102, 412
HpyCH4IV ACGT 3 cut(s) 258, 297, 603
HpyCH4V TGCA 1 cut(s) 362
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 535, 576
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 3 cut(s) 258, 297, 603
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 319, 542
HspAI GCGC 1 cut(s) 448
Kzo9I GATC 1 cut(s) 474
LweI GCATC 2 cut(s) 349, 516
MaeII ACGT 3 cut(s) 258, 297, 603
MaeIII GTNAC 1 cut(s) 541
MalI GATC 1 cut(s) 476
MboI GATC 1 cut(s) 474
MboII GAAGA 9 cut(s) 10, 39, 135, 171, 174, 177, 180, 404, 698
MfeI CAATTG 1 cut(s) 552
MhlI GDGCHC 2 cut(s) 225, 500
MluCI AATT 6 cut(s) 39, 215, 552, 608, 671, 700
MlyI GAGTC 2 cut(s) 185, 192
MnlI CCTC 6 cut(s) 126, 247, 460, 487, 494, 599
MseI TTAA 4 cut(s) 342, 533, 699, 721
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 552
MwoI GCNNNNNNNGC 6 cut(s) 149, 158, 167, 220, 535, 576
NciI CCSGG 1 cut(s) 195
NdeII GATC 1 cut(s) 474
NlaIII CATG 2 cut(s) 319, 542
NlaIV GGNNCC 2 cut(s) 6, 265
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 1 cut(s) 541
NspI RCATGY 1 cut(s) 542
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 251
PfeI GAWTC 1 cut(s) 126
PflFI GACNNNGTC 1 cut(s) 299
PfoI TCCNGGA 1 cut(s) 193
PleI GAGTC 2 cut(s) 184, 192
PpsI GAGTC 2 cut(s) 184, 192
PshAI GACNNNNGTC 1 cut(s) 554
Psp124BI GAGCTC 1 cut(s) 225
PspN4I GGNNCC 2 cut(s) 6, 265
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 299
RseI CAYNNNNRTG 1 cut(s) 320
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 4 cut(s) 342, 533, 699, 721
Sau3AI GATC 1 cut(s) 474
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 185, 192
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 2 cut(s) 225, 500
SfaNI GCATC 2 cut(s) 349, 516
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 155
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 1 cut(s) 320
SmlI CTYRAG 3 cut(s) 251, 365, 499
SmoI CTYRAG 3 cut(s) 251, 365, 499
Sse9I AATT 6 cut(s) 39, 215, 552, 608, 671, 700
SsiI CCGC 1 cut(s) 141
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 2 cut(s) 102, 412
TaiI ACGT 3 cut(s) 261, 300, 606
TaqI TCGA 3 cut(s) 155, 252, 294
TasI AATT 6 cut(s) 39, 215, 552, 608, 671, 700
TfiI GAWTC 1 cut(s) 126
Tru1I TTAA 4 cut(s) 342, 533, 699, 721
Tru9I TTAA 4 cut(s) 342, 533, 699, 721
TscAI CASTG 2 cut(s) 66, 427
TseFI GTSAC 1 cut(s) 541
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 5 cut(s) 32, 332, 405, 678, 706
TspRI CASTG 2 cut(s) 66, 427
Tth111I GACNNNGTC 1 cut(s) 299
XapI RAATTY 2 cut(s) 608, 671
XceI RCATGY 1 cut(s) 542
XcmI CCANNNNNNNNNTGG 2 cut(s) 241, 319
XhoI CTCGAG 1 cut(s) 251
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.