Rmu_sc0001970.1_g000014

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001970.1
OG0003559
Physical Location & Seq
Forward (+)
78078 .. 80442
2365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001970.1_g000014.1.cds

Sequence Viewer

Length: 615 bp
atgaggagactaactaaagctaatattcgcatactttcaaaagaaaatcttcccaaaattgcatctgaagatgatgaaatggtgcagatttctggagaccttgatattgccaaggaagctcttatacatgttgtgacgcgattgagggcaaaccttttcgacagggaaggtgcagtgtctgcattccttccaacagttctgccttatcttcctgtgggaggagatgcggcagatggtccaaattatgacagtagagatggtaaacgacatgggcgttatggtggttatggtggctcaagtgattttggagctaatgacagttatggaagttatggtggttcacaggttggtggtagcggcagtccctatggagcttatggagcttattcttctggacgtactggtagttctgggttatctagccagaaccctgtttctcggcggagaaactatggtaagtgttggggttgtcttgaatggatgcatgaaactttggatctgattccaggagatctgtttgggaacttgtggtgtttgttgaaacaatgtgatcccactggtaggatgatttatctattggcaggttctatgaacgttgttggacgctcaatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

204

Amino Acids

21.95

Weight (kDa)

7.65

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 572
AccII CGCG 1 cut(s) 139
AciI CCGC 3 cut(s) 227, 357, 442
AclI AACGTT 1 cut(s) 594
AclWI GGATC 2 cut(s) 504, 545
AcuI CTGAAG 1 cut(s) 87
AfaI GTAC 1 cut(s) 400
AfiI CCNNNNNNNGG 2 cut(s) 218, 561
AflIII ACRYGT 1 cut(s) 127
AgsI TTSAA 3 cut(s) 39, 476, 541
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 5 cut(s) 20, 119, 311, 374, 383
AluI AGCT 5 cut(s) 20, 119, 311, 374, 383
Alw26I GTCTC 1 cut(s) 90
AlwI GGATC 2 cut(s) 504, 545
AlwNI CAGNNNCTG 1 cut(s) 179
ArsI GACNNNNNNTTYG 1 cut(s) 594
Asp700I GAANNNNTTC 1 cut(s) 48
AspS9I GGNCC 1 cut(s) 236
AvaII GGWCC 1 cut(s) 236
BarI GAAGNNNNNNTAC 2 cut(s) 108, 140
BccI CCATC 2 cut(s) 227, 251
BciT130I CCWGG 1 cut(s) 507
BcoDI GTCTC 1 cut(s) 90
BfaI CTAG 1 cut(s) 420
BfuAI ACCTGC 1 cut(s) 572
BglII AGATCT 1 cut(s) 511
BisI GCNGC 2 cut(s) 228, 358
BlsI GCNGC 2 cut(s) 229, 359
Bme1390I CCNGG 1 cut(s) 507
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 1 cut(s) 236
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 3 cut(s) 71, 214, 471
BpmI CTGGAG 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 280
BsaI GGTCTC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 111
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 561
Bse1I ACTGG 2 cut(s) 406, 562
BseBI CCWGG 1 cut(s) 507
BseDI CCNNGG 1 cut(s) 111
BseGI GGATG 2 cut(s) 486, 570
BseLI CCNNNNNNNGG 2 cut(s) 218, 561
BseNI ACTGG 2 cut(s) 406, 562
BseRI GAGGAG 2 cut(s) 19, 234
BsgI GTGCAG 2 cut(s) 104, 192
Bsh1236I CGCG 1 cut(s) 139
BslFI GGGAC 1 cut(s) 348
BslI CCNNNNNNNGG 2 cut(s) 218, 561
BsmAI GTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 348
BsmI GAATGC 1 cut(s) 182
Bso31I GGTCTC 1 cut(s) 90
Bsp143I GATC 3 cut(s) 496, 511, 550
BspACI CCGC 3 cut(s) 227, 357, 442
BspFNI CGCG 1 cut(s) 139
BspMI ACCTGC 1 cut(s) 572
BspPI GGATC 2 cut(s) 504, 545
BspTNI GGTCTC 1 cut(s) 90
BsrI ACTGG 2 cut(s) 406, 562
BssECI CCNNGG 1 cut(s) 111
BssMI GATC 3 cut(s) 496, 511, 550
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 196, 251, 320
BstAPI GCANNNNNTGC 1 cut(s) 179
BstENI CCTNNNNNAGG 1 cut(s) 216
BstF5I GGATG 2 cut(s) 486, 570
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 3 cut(s) 499, 514, 553
BstMAI GTCTC 1 cut(s) 90
BstMBI GATC 3 cut(s) 496, 511, 550
BstMWI GCNNNNNNNGC 3 cut(s) 116, 179, 380
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 131
BstSCI CCNGG 1 cut(s) 505
BstUI CGCG 1 cut(s) 139
BstX2I RGATCY 2 cut(s) 496, 511
BstYI RGATCY 2 cut(s) 496, 511
BtsCI GGATG 2 cut(s) 486, 570
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 2 cut(s) 180, 555
BveI ACCTGC 1 cut(s) 572
CaiI CAGNNNCTG 1 cut(s) 179
Cfr13I GGNCC 1 cut(s) 236
CseI GACGC 1 cut(s) 145
Csp6I GTAC 1 cut(s) 399
CviAII CATG 3 cut(s) 128, 269, 485
CviJI RGCY 7 cut(s) 20, 119, 294, 311, 374, 383, 423
CviKI_1 RGCY 7 cut(s) 20, 119, 294, 311, 374, 383, 423
CviQI GTAC 1 cut(s) 399
DpnI GATC 3 cut(s) 498, 513, 552
DpnII GATC 3 cut(s) 496, 511, 550
EciI GGCGGA 1 cut(s) 457
Eco130I CCWWGG 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 90
Eco47I GGWCC 1 cut(s) 236
Eco57I CTGAAG 1 cut(s) 87
EcoNI CCTNNNNNAGG 1 cut(s) 216
EcoRII CCWGG 1 cut(s) 505
EcoT14I CCWWGG 1 cut(s) 111
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 3 cut(s) 131, 272, 488
FalI AAGNNNNNCTT 2 cut(s) 33, 65
FaqI GGGAC 1 cut(s) 348
FatI CATG 3 cut(s) 127, 268, 484
Fnu4HI GCNGC 2 cut(s) 228, 358
FokI GGATG 2 cut(s) 493, 577
Fsp4HI GCNGC 2 cut(s) 228, 358
FspBI CTAG 1 cut(s) 420
GluI GCNGC 2 cut(s) 228, 358
GsuI CTGGAG 1 cut(s) 114
HgaI GACGC 1 cut(s) 145
Hin1II CATG 3 cut(s) 131, 272, 488
HinfI GANTC 1 cut(s) 502
Hpy166II GTNNAC 2 cut(s) 263, 341
Hpy188I TCNGA 2 cut(s) 67, 501
Hpy188III TCNNGA 3 cut(s) 93, 393, 473
Hpy8I GTNNAC 2 cut(s) 263, 341
HpyAV CCTTC 2 cut(s) 161, 197
HpyCH4III ACNGT 3 cut(s) 196, 251, 320
HpyCH4IV ACGT 2 cut(s) 397, 594
HpyCH4V TGCA 5 cut(s) 62, 85, 173, 182, 484
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 179, 380
HpySE526I ACGT 2 cut(s) 397, 594
Hsp92II CATG 3 cut(s) 131, 272, 488
Kzo9I GATC 3 cut(s) 496, 511, 550
LmnI GCTCC 3 cut(s) 308, 371, 380
LweI GCATC 3 cut(s) 71, 214, 471
MaeI CTAG 1 cut(s) 420
MaeII ACGT 2 cut(s) 397, 594
MaeIII GTNAC 1 cut(s) 133
MalI GATC 3 cut(s) 498, 513, 552
MboI GATC 3 cut(s) 496, 511, 550
MboII GAAGA 4 cut(s) 41, 80, 200, 381
MflI RGATCY 2 cut(s) 496, 511
MluCI AATT 3 cut(s) 57, 241, 609
MmeI TCCRAC 2 cut(s) 215, 580
MnlI CCTC 2 cut(s) 138, 212
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 48
MspR9I CCNGG 1 cut(s) 507
Mva1269I GAATGC 1 cut(s) 182
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 3 cut(s) 116, 179, 380
NdeII GATC 3 cut(s) 496, 511, 550
NlaIII CATG 3 cut(s) 131, 272, 488
NmeAIII GCCGAG 1 cut(s) 418
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 1 cut(s) 486
NspI RCATGY 1 cut(s) 131
PciI ACATGT 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 1 cut(s) 182
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 1 cut(s) 502
PfoI TCCNGGA 1 cut(s) 505
PkrI GCNGC 2 cut(s) 229, 359
PscI ACATGT 1 cut(s) 127
Psp1406I AACGTT 1 cut(s) 594
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PspPI GGNCC 1 cut(s) 236
PsrI GAACNNNNNNTAC 2 cut(s) 391, 423
PstNI CAGNNNCTG 1 cut(s) 179
PsuI RGATCY 2 cut(s) 496, 511
RsaI GTAC 1 cut(s) 400
RsaNI GTAC 1 cut(s) 399
SatI GCNGC 2 cut(s) 228, 358
Sau3AI GATC 3 cut(s) 496, 511, 550
Sau96I GGNCC 1 cut(s) 236
ScrFI CCNGG 1 cut(s) 507
SfaNI GCATC 3 cut(s) 71, 214, 471
SinI GGWCC 1 cut(s) 236
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
Sse9I AATT 3 cut(s) 57, 241, 609
SsiI CCGC 3 cut(s) 227, 357, 442
SspI AATATT 1 cut(s) 25
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 196, 251, 320
TaiI ACGT 2 cut(s) 400, 597
TaqI TCGA 1 cut(s) 159
TasI AATT 3 cut(s) 57, 241, 609
TauI GCSGC 2 cut(s) 230, 360
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 2 cut(s) 180, 562
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 3 cut(s) 90, 501, 605
TspRI CASTG 2 cut(s) 180, 562
VpaK11BI GGWCC 1 cut(s) 236
XagI CCTNNNNNAGG 1 cut(s) 216
XceI RCATGY 1 cut(s) 131
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 1 cut(s) 420
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.