Rorug02G0530800

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003559
Physical Location & Seq
Forward (+)
66079300 .. 66085063
5764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0530800.1

Sequence Viewer

Length: 2823 bp
ATGGAGGCCTTCTTGACCCATGTATGCAAGACAAGAACACTGCTTACATTGATCTTATGTATTTGGGTGCCCATGGAAGTGATGGCTCAGACTGGAAATGCCACCGTCTCATCTAAGAGGCCGAGTTCTCTGAATATTGGAGCTCTGTTTACTTTCAATTCAGCCATTGGAAGGTCTGTCAAACCAGCAATCCTAGCCGCAATTGAAGAAGTTAATTCTGATTCAAGCGTTCTCGAGGGGACTAAATTGAACATTATTTTCCATGATACAAATTGCAGTGGATTTCTTGGAACTGTGGAAGCTTTGCAGCTCATTGAAAATGATGTGGTTGCTGCAATTGGCCCACAGTCCTCAGGAATAGCTCATGTCATATCCCACGTTGTTAATGAGCTCCATGTACCACTTCTGTCATTTGGAGCAACAGACCCCTCCCTTTCTGCTCTACAATACCCGTATTTTGTACGCACCACGCAGAGTGACTCTTTTCAAATGTATGCAGTTGCTGATATGGTTGAGTATTTCGGATGGAGAGAAGTAATTGCCATATTTGTAGATGATGATTGTGGCAGAAATGGGATTTCAGTACTAGGTGATGCCTTAGCAAAGAAGCGTTCCAAGATTGCTTACAAGGCTGCCTTCTCTCCTGGAGCCCCATTAAGTGATATCAATGACTTGTTGGTGGGAGTAAACCTCATGGAATCTCGGGTGTATATTGTACATGTAAATCCTGACTCTGGTTTGTCAGTCTTTTCTGTTGCCAAGGATCTTGGAATGATGACAAGTGGCTATGTTTGGATTGCAACGGATTGGCTTCCTACTAATTTAGATTCATTAGTACCACCTGACCCTGACAAAATGAATCTTTTACAAGGGGTTGTTGCTCTTCGTCATCATTCCCCGGATACTGATCTCAAAAAGAGTTTTGCATCTAAATGGAGAAAACTAAAGCATGAAGGGAGTCCGGGCTTCAATTCTTATGCTCTCTATGCATATGACTCTATTTGGTTAGCAGCCCGTGCCCTTGATGTTTTTCTCAATGAAGGAGGGACTGTATCTTTCTCTAGTGACCCAAAGTTGAAAGATACAAATAGAAGCGCACTGCACTTGGCATCACTCCGAATATTTGATGGAGGCCAACATTACCTGCAGACACTTCTTAAGATGAACTTCACAGGTGTGAGTGGTCAGCTTCAGTTTGATCTGGATAAGAATTTAATTCATCCAGCTTATGATGTTCTGAACATTGGTGGAACTGGATTTCGTAAAATTGGTTATTGGTCAAATTCTACTGGTCTGTCGACCACTGAGCCAGAGACATTATTTGGGAAGTCGTTCAATACTTCTACCAGAACTCCACATCTTTATAGTGTTATATGGCCTGGTGAAACTATAGCTACACCTCGGGGATGGGTATTTCCTAACAATGGCAAGCCACTGCGTATTGCTGTGCCTGAGAGAGTAGGTTACAAAGAGTTTGTGTCTAAAGACAAGAGTCCTCCTGGGGTCAGAGGATATTGTATTGATGTATTTGAAGCTGCTATAAACTTGTTGCCTTATCCTGTGCCACGCACATATATGTTGTATGGAGGTGGCAAGAGGAATCCCGATTACAACAATCTTGTTGGTCAAGTTGCTCTAAATAACTTTGATGCAGCTGTTGGGGATGTTACAATAACTACAAATAGGACAAGAATGGTTGATTTTACACAGCCTTACATGGAATCAGGACTAGTTGTAGTTGTTCCTGTCAAACAGTTAAAATCAAACCCTTGGGCTTTCCTGAAGCCATTTACTATTGAGATGTGGTTAGTCACCGGTGCATTCTTCCTTTTTGTGGGAGTCGTTGTTTGGATTCTTGAGCACAGGATTAATCACGAGTTCCGTGGTCCACCAAGGGAACAGCTCATGACTATTTTTTGGTTTAGTTTCTCGACGATGTTCTTCTCACATAGAGAGAACACTGTGAGCACTCTAGGACGGCTGGTGCTGATTATATGGTTGTTTGTGGTGTTGATTATCAATTCGAGCTATACAGCTAGTTTGACATCGATCCTCACTGTACAACAGCTGACATCACGGATTGAGGGGATAGACAGCTTGATATCAAGTAATGATCCAATCGGAGTTCAAGATGGGTCATTTTCATGGAAATATTTGGTTAATGAGCTCAACATAGCGGAGTCGAGACTTGTTACCTTAAAAACCATGGAAGACTACATCAAAGCCCTTCAGGATGGACCAAAACGTGGTGGAGTAGCTGCCATTGTCGATGAGCTTCCTTACATTGAGCTGTTTATGTCTGCCACCAAGTGTGCATTCAGGACTGTGGGGCAGGAATTTACAAAAAGCGGATGGGGATTTGCATTCCAAAGGGATTCTCCTCTTGCTGTTGATTTGTCAACTGCCATTCTTCAACTCTCGGAGAATGGTGACCTCCAAAAGATCCGTAATAAATGGCTTAGGAGTAGCGAGTGCTCTATGCAACTCAATGAAATGGATGCAGACCGACTATCCCTGACGAGCTTTTGGGGCCTGTTCCTTATATGCGGTATTGCATGCTTCATTGCTCTTACTGTGTTCTTCTGTAGAATCTTATTGCAATACCGCAGATTTAGTCCAGAGACTCTGGAAGGGGATGTTGAGGAAATTGAACCTCCTCCCAGATCTAGTCGCCGCTCACTCCGGACAACTAGTTTCAAAAACTTGATGGATTTTGTAGATAAGAAAGAAGTAGAGATCAAGCATATGCTAAAGAGAAAGAGCAGCGATAACAAACAAGAAGCTAGTCCAAGCTCTGATGGCAAGTCCCTTTCACCCTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

940

Amino Acids

104.53

Weight (kDa)

6.52

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 52 - 342 5.8e-19 Periplasmic binding protein
ANF_receptor PF01094 60 - 417 5.8e-89 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 501 - 583 8.7e-11 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 536 - 821 2.9e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 599 - 853 1.1e-39 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1152
AccB1I GGYRCC 1 cut(s) 67
AccB7I CCANNNNNTGG 1 cut(s) 2246
AccBSI CCGCTC 1 cut(s) 2676
AccI GTMKAC 1 cut(s) 1298
AccIII TCCGGA 1 cut(s) 2682
AciI CCGC 6 cut(s) 198, 2177, 2349, 2547, 2605, 2674
AclWI GGATC 4 cut(s) 771, 2044, 2108, 2437
AcsI RAATTY 3 cut(s) 1210, 1282, 2336
AcuI CTGAAG 3 cut(s) 1175, 1802, 2213
AdeI CACNNNGTG 1 cut(s) 2310
AfaI GTAC 6 cut(s) 399, 462, 585, 717, 837, 2061
AfiI CCNNNNNNNGG 5 cut(s) 171, 734, 1424, 1834, 2246
AflII CTTAAG 1 cut(s) 1157
AflIII ACRYGT 1 cut(s) 718
AgeI ACCGGT 1 cut(s) 1814
AhlI ACTAGT 2 cut(s) 1729, 2690
AjnI CCWGG 3 cut(s) 643, 1378, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 2300, 2332
AleI CACNNNNGTG 1 cut(s) 1175
Alw21I GWGCWC 6 cut(s) 145, 393, 1863, 1970, 2169, 2477
Alw26I GTCTC 4 cut(s) 112, 1307, 2179, 2615
AlwI GGATC 4 cut(s) 771, 2044, 2108, 2437
AlwNI CAGNNNCTG 1 cut(s) 503
Ama87I CYCGRG 3 cut(s) 233, 702, 1401
Aor13HI TCCGGA 1 cut(s) 2682
AoxI GGCC 6 cut(s) 6, 119, 340, 1132, 1376, 2530
ApeKI GCWGC 8 cut(s) 307, 332, 632, 1010, 1535, 1652, 2258, 2763
ApoI RAATTY 3 cut(s) 1210, 1282, 2336
AseI ATTAAT 1 cut(s) 1869
AsiGI ACCGGT 1 cut(s) 1814
Asp700I GAANNNNTTC 1 cut(s) 1331
AspLEI GCGC 1 cut(s) 1097
AspS9I GGNCC 4 cut(s) 341, 1886, 2237, 2530
AsuC2I CCSGG 2 cut(s) 899, 963
AsuHPI GGTGA 5 cut(s) 602, 1394, 1804, 2441, 2805
AvaI CYCGRG 3 cut(s) 233, 702, 1401
AvaII GGWCC 2 cut(s) 1886, 2237
AxyI CCTNAGG 1 cut(s) 352
BaeGI GKGCMC 2 cut(s) 72, 1021
BanI GGYRCC 1 cut(s) 67
BanII GRGCYC 4 cut(s) 145, 393, 652, 2169
BauI CACGAG 1 cut(s) 1874
BbsI GAAGAC 1 cut(s) 2217
Bbv12I GWGCWC 6 cut(s) 145, 393, 1863, 1970, 2169, 2477
BbvI GCAGC 8 cut(s) 319, 319, 619, 1022, 1522, 1664, 2245, 2775
BccI CCATC 9 cut(s) 76, 519, 1121, 1401, 2126, 2228, 2346, 2701, 2792
BceAI ACGGC 1 cut(s) 1994
BciT130I CCWGG 3 cut(s) 645, 1380, 1500
BciVI GTATCC 1 cut(s) 895
BcnI CCSGG 2 cut(s) 899, 963
BcoDI GTCTC 4 cut(s) 112, 1307, 2179, 2615
BcuI ACTAGT 2 cut(s) 1729, 2690
BfaI CTAG 9 cut(s) 194, 587, 1062, 1730, 1973, 2037, 2667, 2691, 2784
BfmI CTRYAG 3 cut(s) 1145, 1389, 2584
BfrI CTTAAG 1 cut(s) 1157
BfuAI ACCTGC 1 cut(s) 1152
BfuI GTATCC 1 cut(s) 895
BglII AGATCT 1 cut(s) 2663
BmcAI AGTACT 1 cut(s) 585
Bme1390I CCNGG 5 cut(s) 645, 899, 963, 1380, 1500
Bme18I GGWCC 2 cut(s) 1886, 2237
BmeT110I CYCGRG 3 cut(s) 233, 702, 1401
BmgT120I GGNCC 4 cut(s) 341, 1886, 2237, 2530
BmiI GGNNCC 3 cut(s) 69, 649, 2531
BmrFI CCNGG 5 cut(s) 645, 899, 963, 1380, 1500
BmsI GCATC 5 cut(s) 583, 935, 1118, 1639, 2488
BoxI GACNNNNGTC 1 cut(s) 1491
BpiI GAAGAC 1 cut(s) 2217
BplI GAGNNNNNCTC 2 cut(s) 675, 707
BpmI CTGGAG 1 cut(s) 666
Bpu10I CCTNAGC 2 cut(s) 598, 2459
BpuEI CTTGAG 1 cut(s) 1877
BpuMI CCSGG 2 cut(s) 899, 963
Bsa29I ATCGAT 1 cut(s) 2048
BsaBI GATNNNNATC 1 cut(s) 906
BsaJI CCNNGG 9 cut(s) 72, 759, 897, 1400, 1499, 1769, 1882, 1892, 2205
BsaWI WCCGGW 2 cut(s) 1814, 2682
BsaXI ACNNNNNCTCC 6 cut(s) 1035, 1065, 1336, 1366, 1830, 1860
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 734, 1424, 1834, 2246
Bse118I RCCGGY 1 cut(s) 1814
Bse1I ACTGG 3 cut(s) 97, 1258, 1294
Bse21I CCTNAGG 1 cut(s) 352
Bse3DI GCAATG 1 cut(s) 2562
Bse8I GATNNNNATC 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 2682
BseBI CCWGG 3 cut(s) 645, 1380, 1500
BseCI ATCGAT 1 cut(s) 2048
BseDI CCNNGG 9 cut(s) 72, 759, 897, 1400, 1499, 1769, 1882, 1892, 2205
BseGI GGATG 8 cut(s) 530, 1219, 1412, 1669, 2239, 2357, 2503, 2641
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 5 cut(s) 171, 734, 1424, 1834, 2246
BseMI GCAATG 1 cut(s) 2562
BseMII CTCAG 4 cut(s) 101, 366, 1296, 1443
BseNI ACTGG 3 cut(s) 97, 1258, 1294
BseRI GAGGAG 2 cut(s) 2370, 2646
BseSI GKGCMC 2 cut(s) 72, 1021
BseXI GCAGC 8 cut(s) 319, 319, 619, 1022, 1522, 1664, 2245, 2775
BsgI GTGCAG 1 cut(s) 1085
BshFI GGCC 6 cut(s) 8, 121, 342, 1134, 1378, 2532
BshNI GGYRCC 1 cut(s) 67
BshTI ACCGGT 1 cut(s) 1814
BshVI ATCGAT 1 cut(s) 2048
BsiHKAI GWGCWC 6 cut(s) 145, 393, 1863, 1970, 2169, 2477
BsiHKCI CYCGRG 3 cut(s) 233, 702, 1401
BsiSI CCGG 4 cut(s) 899, 962, 1815, 2683
BslFI GGGAC 3 cut(s) 253, 1060, 2791
BslI CCNNNNNNNGG 5 cut(s) 171, 734, 1424, 1834, 2246
BsmAI GTCTC 4 cut(s) 112, 1307, 2179, 2615
BsmBI CGTCTC 1 cut(s) 112
BsmFI GGGAC 3 cut(s) 253, 1060, 2791
BsmI GAATGC 3 cut(s) 1820, 2315, 2363
BsnI GGCC 6 cut(s) 8, 121, 342, 1134, 1378, 2532
BsoBI CYCGRG 3 cut(s) 233, 702, 1401
Bsp1286I GDGCHC 9 cut(s) 72, 145, 393, 652, 1021, 1863, 1970, 2169, 2477
Bsp13I TCCGGA 1 cut(s) 2682
Bsp1407I TGTACA 2 cut(s) 715, 2059
Bsp143I GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
Bsp19I CCATGG 2 cut(s) 72, 2205
BspACI CCGC 6 cut(s) 198, 2177, 2349, 2547, 2605, 2674
BspANI GGCC 6 cut(s) 8, 121, 342, 1134, 1378, 2532
BspCNI CTCAG 4 cut(s) 100, 365, 1297, 1444
BspDI ATCGAT 1 cut(s) 2048
BspEI TCCGGA 1 cut(s) 2682
BspHI TCATGA 1 cut(s) 1905
BspLI GGNNCC 3 cut(s) 69, 649, 2531
BspMAI CTGCAG 1 cut(s) 1149
BspMI ACCTGC 1 cut(s) 1152
BspPI GGATC 4 cut(s) 771, 2044, 2108, 2437
BspQI GCTCTTC 1 cut(s) 888
BspT107I GGYRCC 1 cut(s) 67
BspTI CTTAAG 1 cut(s) 1157
BsrBI CCGCTC 1 cut(s) 2676
BsrDI GCAATG 1 cut(s) 2562
BsrFI RCCGGY 1 cut(s) 1814
BsrGI TGTACA 2 cut(s) 715, 2059
BsrI ACTGG 3 cut(s) 97, 1258, 1294
BssAI RCCGGY 1 cut(s) 1814
BssECI CCNNGG 9 cut(s) 72, 759, 897, 1400, 1499, 1769, 1882, 1892, 2205
BssMI GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
BssSI CACGAG 1 cut(s) 1874
BssT1I CCWWGG 5 cut(s) 72, 759, 1769, 1892, 2205
Bst2BI CACGAG 1 cut(s) 1874
Bst2UI CCWGG 3 cut(s) 645, 1380, 1500
Bst4CI ACNGT 9 cut(s) 106, 295, 348, 1051, 1755, 1963, 2059, 2326, 2575
Bst6I CTCTTC 1 cut(s) 888
BstAFI CTTAAG 1 cut(s) 1157
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstAUI TGTACA 2 cut(s) 715, 2059
BstC8I GCNNGC 2 cut(s) 1430, 2557
BstDEI CTNAG 7 cut(s) 87, 114, 352, 598, 1305, 1452, 2459
BstDSI CCRYGG 3 cut(s) 72, 1882, 2205
BstEII GGTNACC 1 cut(s) 2429
BstF5I GGATG 8 cut(s) 530, 1219, 1412, 1669, 2239, 2357, 2503, 2641
BstHHI GCGC 1 cut(s) 1097
BstKTI GATC 9 cut(s) 54, 766, 910, 1201, 2052, 2116, 2445, 2666, 2739
BstMAI GTCTC 4 cut(s) 112, 1307, 2179, 2615
BstMBI GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
BstMWI GCNNNNNNNGC 6 cut(s) 194, 629, 986, 1016, 2529, 2799
BstNI CCWGG 3 cut(s) 645, 1380, 1500
BstNSI RCATGY 2 cut(s) 722, 2559
BstPAI GACNNNNGTC 1 cut(s) 1491
BstPI GGTNACC 1 cut(s) 2429
BstSCI CCNGG 5 cut(s) 643, 897, 961, 1378, 1498
BstSFI CTRYAG 3 cut(s) 1145, 1389, 2584
BstSLI GKGCMC 2 cut(s) 72, 1021
BstV1I GCAGC 8 cut(s) 319, 319, 619, 1022, 1522, 1664, 2245, 2775
BstV2I GAAGAC 1 cut(s) 2217
BstX2I RGATCY 3 cut(s) 763, 2442, 2663
BstYI RGATCY 3 cut(s) 763, 2442, 2663
Bsu15I ATCGAT 1 cut(s) 2048
Bsu36I CCTNAGG 1 cut(s) 352
BsuI GTATCC 1 cut(s) 895
BsuRI GGCC 6 cut(s) 8, 121, 342, 1134, 1378, 2532
BsuTUI ATCGAT 1 cut(s) 2048
BtgI CCRYGG 3 cut(s) 72, 1882, 2205
BtsCI GGATG 8 cut(s) 530, 1219, 1412, 1669, 2239, 2357, 2503, 2641
BtsI GCAGTG 4 cut(s) 38, 283, 1097, 1433
BtsIMutI CAGTG 7 cut(s) 38, 283, 1097, 1302, 1433, 1959, 2055
BveI ACCTGC 1 cut(s) 1152
Cac8I GCNNGC 2 cut(s) 1430, 2557
CaiI CAGNNNCTG 1 cut(s) 503
CciI TCATGA 1 cut(s) 1905
CfoI GCGC 1 cut(s) 1097
Cfr10I RCCGGY 1 cut(s) 1814
Cfr13I GGNCC 4 cut(s) 341, 1886, 2237, 2530
ClaI ATCGAT 1 cut(s) 2048
Csp6I GTAC 6 cut(s) 398, 461, 584, 716, 836, 2060
CspAI ACCGGT 1 cut(s) 1814
CviQI GTAC 6 cut(s) 398, 461, 584, 716, 836, 2060
DdeI CTNAG 7 cut(s) 87, 114, 352, 598, 1305, 1452, 2459
DpnI GATC 9 cut(s) 53, 765, 909, 1200, 2051, 2115, 2444, 2665, 2738
DpnII GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
DraIII CACNNNGTG 1 cut(s) 2310
Eam1104I CTCTTC 1 cut(s) 888
EarI CTCTTC 1 cut(s) 888
Ecl136II GAGCTC 3 cut(s) 143, 391, 2167
Eco130I CCWWGG 5 cut(s) 72, 759, 1769, 1892, 2205
Eco147I AGGCCT 1 cut(s) 8
Eco24I GRGCYC 4 cut(s) 145, 393, 652, 2169
Eco32I GATATC 2 cut(s) 664, 2103
Eco47I GGWCC 2 cut(s) 1886, 2237
Eco53kI GAGCTC 3 cut(s) 143, 391, 2167
Eco57I CTGAAG 3 cut(s) 1175, 1802, 2213
Eco81I CCTNAGG 1 cut(s) 352
Eco88I CYCGRG 3 cut(s) 233, 702, 1401
Eco91I GGTNACC 1 cut(s) 2429
EcoICRI GAGCTC 3 cut(s) 143, 391, 2167
EcoO109I RGGNCCY 1 cut(s) 2530
EcoO65I GGTNACC 1 cut(s) 2429
EcoRII CCWGG 3 cut(s) 643, 1378, 1498
EcoRV GATATC 2 cut(s) 664, 2103
EcoT14I CCWWGG 5 cut(s) 72, 759, 1769, 1892, 2205
EcoT22I ATGCAT 1 cut(s) 991
EcoT38I GRGCYC 4 cut(s) 145, 393, 652, 2169
ErhI CCWWGG 5 cut(s) 72, 759, 1769, 1892, 2205
Esp3I CGTCTC 1 cut(s) 112
FalI AAGNNNNNCTT 4 cut(s) 620, 652, 1151, 1183
FaqI GGGAC 3 cut(s) 253, 1060, 2791
FauNDI CATATG 2 cut(s) 991, 2745
FblI GTMKAC 1 cut(s) 1298
FokI GGATG 8 cut(s) 537, 1206, 1419, 1676, 2246, 2364, 2510, 2648
FriOI GRGCYC 4 cut(s) 145, 393, 652, 2169
FspBI CTAG 9 cut(s) 194, 587, 1062, 1730, 1973, 2037, 2667, 2691, 2784
GlaI GCGC 1 cut(s) 1096
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 6 cut(s) 8, 121, 342, 1134, 1378, 2532
HapII CCGG 4 cut(s) 899, 962, 1815, 2683
HhaI GCGC 1 cut(s) 1097
Hin6I GCGC 1 cut(s) 1095
HinP1I GCGC 1 cut(s) 1095
HincII GTYRAC 2 cut(s) 1299, 2400
HindII GTYRAC 2 cut(s) 1299, 2400
HindIII AAGCTT 1 cut(s) 300
HpaII CCGG 4 cut(s) 899, 962, 1815, 2683
HphI GGTGA 5 cut(s) 602, 1394, 1804, 2441, 2805
Hpy166II GTNNAC 5 cut(s) 150, 688, 1299, 1889, 2400
Hpy8I GTNNAC 5 cut(s) 150, 688, 1299, 1889, 2400
Hpy99I CGWCG 1 cut(s) 1936
HpyAV CCTTC 7 cut(s) 19, 165, 646, 947, 1034, 2237, 2624
HpyCH4III ACNGT 9 cut(s) 106, 295, 348, 1051, 1755, 1963, 2059, 2326, 2575
HpyCH4IV ACGT 2 cut(s) 378, 2245
HpyF10VI GCNNNNNNNGC 6 cut(s) 194, 629, 986, 1016, 2529, 2799
HpyF3I CTNAG 7 cut(s) 87, 114, 352, 598, 1305, 1452, 2459
HpySE526I ACGT 2 cut(s) 378, 2245
HspAI GCGC 1 cut(s) 1095
Kpn2I TCCGGA 1 cut(s) 2682
Kzo9I GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
LguI GCTCTTC 1 cut(s) 888
LmnI GCTCC 4 cut(s) 140, 396, 416, 647
Lsp1109I GCAGC 8 cut(s) 319, 319, 619, 1022, 1522, 1664, 2245, 2775
LweI GCATC 5 cut(s) 583, 935, 1118, 1639, 2488
MaeI CTAG 9 cut(s) 194, 587, 1062, 1730, 1973, 2037, 2667, 2691, 2784
MaeII ACGT 2 cut(s) 378, 2245
MaeIII GTNAC 7 cut(s) 476, 1064, 1463, 1666, 1810, 2191, 2429
MalI GATC 9 cut(s) 53, 765, 909, 1200, 2051, 2115, 2444, 2665, 2738
MbiI CCGCTC 1 cut(s) 2676
MboI GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
MboII GAAGA 7 cut(s) 218, 875, 1816, 1933, 2222, 2402, 2572
MfeI CAATTG 2 cut(s) 201, 336
MflI RGATCY 3 cut(s) 763, 2442, 2663
MhlI GDGCHC 9 cut(s) 72, 145, 393, 652, 1021, 1863, 1970, 2169, 2477
MlyI GAGTC 8 cut(s) 473, 725, 967, 989, 1501, 1848, 2189, 2617
Mph1103I ATGCAT 1 cut(s) 991
MroI TCCGGA 1 cut(s) 2682
MroXI GAANNNNTTC 1 cut(s) 1331
MslI CAYNNNNRTG 5 cut(s) 77, 931, 1175, 1574, 2143
MspA1I CMGCKG 2 cut(s) 1655, 2068
MspCI CTTAAG 1 cut(s) 1157
MspI CCGG 4 cut(s) 899, 962, 1815, 2683
MspR9I CCNGG 5 cut(s) 645, 899, 963, 1380, 1500
MunI CAATTG 2 cut(s) 201, 336
Mva1269I GAATGC 3 cut(s) 1820, 2315, 2363
MvaI CCWGG 3 cut(s) 645, 1380, 1500
MwoI GCNNNNNNNGC 6 cut(s) 194, 629, 986, 1016, 2529, 2799
NciI CCSGG 2 cut(s) 899, 963
NcoI CCATGG 2 cut(s) 72, 2205
NdeI CATATG 2 cut(s) 991, 2745
NdeII GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
NlaIV GGNNCC 3 cut(s) 69, 649, 2531
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 4 cut(s) 476, 1064, 1810, 2429
NsiI ATGCAT 1 cut(s) 991
NspI RCATGY 2 cut(s) 722, 2559
OliI CACNNNNGTG 1 cut(s) 1175
PaeI GCATGC 1 cut(s) 2559
PaeR7I CTCGAG 1 cut(s) 233
PagI TCATGA 1 cut(s) 1905
PceI AGGCCT 1 cut(s) 8
PciI ACATGT 1 cut(s) 718
PciSI GCTCTTC 1 cut(s) 888
PctI GAATGC 3 cut(s) 1820, 2315, 2363
PdmI GAANNNNTTC 1 cut(s) 1331
PfeI GAWTC 9 cut(s) 221, 698, 827, 859, 1600, 1721, 1852, 2375, 2589
PflMI CCANNNNNTGG 1 cut(s) 2246
PfoI TCCNGGA 1 cut(s) 643
PinAI ACCGGT 1 cut(s) 1814
PleI GAGTC 8 cut(s) 473, 725, 966, 989, 1500, 1847, 2188, 2617
PpsI GAGTC 8 cut(s) 473, 725, 966, 989, 1500, 1847, 2188, 2617
PscI ACATGT 1 cut(s) 718
PshAI GACNNNNGTC 1 cut(s) 1491
PshBI ATTAAT 1 cut(s) 1869
Psp124BI GAGCTC 3 cut(s) 145, 393, 2169
Psp6I CCWGG 3 cut(s) 643, 1378, 1498
PspEI GGTNACC 1 cut(s) 2429
PspGI CCWGG 3 cut(s) 643, 1378, 1498
PspN4I GGNNCC 3 cut(s) 69, 649, 2531
PspPI GGNCC 4 cut(s) 341, 1886, 2237, 2530
PsrI GAACNNNNNNTAC 2 cut(s) 28, 60
PstI CTGCAG 1 cut(s) 1149
PstNI CAGNNNCTG 1 cut(s) 503
PsuI RGATCY 3 cut(s) 763, 2442, 2663
PvuII CAGCTG 2 cut(s) 1655, 2068
RsaI GTAC 6 cut(s) 399, 462, 585, 717, 837, 2061
RsaNI GTAC 6 cut(s) 398, 461, 584, 716, 836, 2060
RseI CAYNNNNRTG 5 cut(s) 77, 931, 1175, 1574, 2143
SacI GAGCTC 3 cut(s) 145, 393, 2169
SalI GTCGAC 1 cut(s) 1297
SapI GCTCTTC 1 cut(s) 888
Sau3AI GATC 9 cut(s) 51, 763, 907, 1198, 2049, 2113, 2442, 2663, 2736
Sau96I GGNCC 4 cut(s) 341, 1886, 2237, 2530
ScaI AGTACT 1 cut(s) 585
SchI GAGTC 8 cut(s) 473, 725, 967, 989, 1501, 1848, 2189, 2617
ScrFI CCNGG 5 cut(s) 645, 899, 963, 1380, 1500
SduI GDGCHC 9 cut(s) 72, 145, 393, 652, 1021, 1863, 1970, 2169, 2477
SfaNI GCATC 5 cut(s) 583, 935, 1118, 1639, 2488
SfcI CTRYAG 3 cut(s) 1145, 1389, 2584
Sfr274I CTCGAG 1 cut(s) 233
SgrAI CRCCGGYG 1 cut(s) 1814
SinI GGWCC 2 cut(s) 1886, 2237
SlaI CTCGAG 1 cut(s) 233
SmiMI CAYNNNNRTG 5 cut(s) 77, 931, 1175, 1574, 2143
SmlI CTYRAG 3 cut(s) 233, 1157, 1856
SmoI CTYRAG 3 cut(s) 233, 1157, 1856
SpeI ACTAGT 2 cut(s) 1729, 2690
SphI GCATGC 1 cut(s) 2559
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 6 cut(s) 198, 2177, 2349, 2547, 2605, 2674
SspI AATATT 3 cut(s) 136, 1122, 2153
SspMI CTAG 9 cut(s) 194, 587, 1062, 1730, 1973, 2037, 2667, 2691, 2784
SstI GAGCTC 3 cut(s) 145, 393, 2169
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 5 cut(s) 643, 897, 961, 1378, 1498
StyI CCWWGG 5 cut(s) 72, 759, 1769, 1892, 2205
TaaI ACNGT 9 cut(s) 106, 295, 348, 1051, 1755, 1963, 2059, 2326, 2575
TaiI ACGT 2 cut(s) 381, 2248
TaqI TCGA 7 cut(s) 234, 1298, 1931, 2024, 2048, 2183, 2268
TaqII GACCGA 1 cut(s) 2520
TatI WGTACW 3 cut(s) 583, 715, 2059
TauI GCSGC 2 cut(s) 200, 2676
TfiI GAWTC 9 cut(s) 221, 698, 827, 859, 1600, 1721, 1852, 2375, 2589
TscAI CASTG 7 cut(s) 45, 283, 1104, 1309, 1440, 1966, 2062
TseFI GTSAC 4 cut(s) 476, 1064, 1810, 2429
TseI GCWGC 8 cut(s) 307, 332, 632, 1010, 1535, 1652, 2258, 2763
Tsp45I GTSAC 4 cut(s) 476, 1064, 1810, 2429
TspDTI ATGAA 9 cut(s) 819, 872, 966, 1053, 1178, 1208, 2133, 2505, 2551
TspGWI ACGGA 4 cut(s) 818, 1871, 2092, 2435
TspRI CASTG 7 cut(s) 45, 283, 1104, 1309, 1440, 1966, 2062
Van91I CCANNNNNTGG 1 cut(s) 2246
Vha464I CTTAAG 1 cut(s) 1157
VpaK11BI GGWCC 2 cut(s) 1886, 2237
VspI ATTAAT 1 cut(s) 1869
XapI RAATTY 3 cut(s) 1210, 1282, 2336
XceI RCATGY 2 cut(s) 722, 2559
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XhoI CTCGAG 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 1298
XmnI GAANNNNTTC 1 cut(s) 1331
XspI CTAG 9 cut(s) 194, 587, 1062, 1730, 1973, 2037, 2667, 2691, 2784
ZrmI AGTACT 1 cut(s) 585
Zsp2I ATGCAT 1 cut(s) 991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.