Rorug02G0530900

KH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0003559
Physical Location & Seq
Reverse (-)
66097799 .. 66098338
540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0530900.1

Sequence Viewer

Length: 405 bp
ATGGCACCTTCAAGACTCTCCCTTTCCCTCGCTCTGCTCCTCCTCGCCGTCTGGGGCTTCGGCCACGACCACTCCGCCTCCTCCCGCGGCGCCCCTGCTCCGGCGGTGGACTGCTCGTCGTTGATCCTGAACATGGCGGACTGCTTGTCGTTTGTCTCCAACGAAAGCAAGGCCGAGAAGCCGGAAGGGAGTTGCTGCACTGGACTCAAGACTATGCTCAAAGCTGACGCCGCTTGTCTCTGTGAGGCCTTCAAGAGCAGCGCCCAGTTGGGTGTCGTCTTGAACATCACTAAGGCCATGTCCCTCCCCGCCGCTTGCAAAGTCTCCGCCCCCTCCGCCACCAACTGTGGATTGTCTGTTTCTCCTGCTGGTGCTCCTGGTATGTTCTTCTTCTTAACTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KH_1

Protein Analysis

134

Amino Acids

13.6

Weight (kDa)

7.53

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LTP_2 PF14368 32 - 116 9.3e-18 Probable lipid transfer
Tryp_alpha_amyl PF00234 38 - 116 2e-13 Protease inhibitor/seed storage/LTP family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 4, 89
AccII CGCG 1 cut(s) 87
AclWI GGATC 1 cut(s) 118
AcoI YGGCCR 1 cut(s) 61
AcyI GRCGYC 2 cut(s) 90, 228
AfiI CCNNNNNNNGG 2 cut(s) 100, 133
AgsI TTSAA 3 cut(s) 12, 253, 283
AhdI GACNNNNNGTC 2 cut(s) 115, 145
AhlI ACTAGT 1 cut(s) 398
AjnI CCWGG 1 cut(s) 376
AluBI AGCT 1 cut(s) 224
AluI AGCT 1 cut(s) 224
Alw21I GWGCWC 1 cut(s) 376
Alw26I GTCTC 3 cut(s) 160, 242, 328
AlwI GGATC 1 cut(s) 118
AoxI GGCC 4 cut(s) 61, 171, 246, 294
ApeKI GCWGC 2 cut(s) 195, 258
AspLEI GCGC 2 cut(s) 92, 263
BanI GGYRCC 2 cut(s) 4, 89
Bbv12I GWGCWC 1 cut(s) 376
BbvI GCAGC 2 cut(s) 182, 270
BceAI ACGGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 378
BcoDI GTCTC 3 cut(s) 160, 242, 328
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 1 cut(s) 399
BfoI RGCGCY 2 cut(s) 93, 264
BisI GCNGC 5 cut(s) 88, 196, 231, 259, 312
BlsI GCNGC 5 cut(s) 89, 197, 232, 260, 313
Bme1390I CCNGG 1 cut(s) 378
BmeRI GACNNNNNGTC 2 cut(s) 115, 145
BmiI GGNNCC 2 cut(s) 6, 91
BmrFI CCNGG 1 cut(s) 378
BmrI ACTGGG 1 cut(s) 259
BmuI ACTGGG 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 191
BsaHI GRCGYC 2 cut(s) 90, 228
BsaJI CCNNGG 1 cut(s) 85
BsaXI ACNNNNNCTCC 4 cut(s) 56, 62, 86, 92
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 133
Bse1I ACTGG 2 cut(s) 205, 265
BseBI CCWGG 1 cut(s) 378
BseDI CCNNGG 1 cut(s) 85
BseLI CCNNNNNNNGG 2 cut(s) 100, 133
BseNI ACTGG 2 cut(s) 205, 265
BseRI GAGGAG 3 cut(s) 29, 32, 70
BseXI GCAGC 2 cut(s) 182, 270
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 87
BshFI GGCC 4 cut(s) 63, 173, 248, 296
BshNI GGYRCC 2 cut(s) 4, 89
BsiHKAI GWGCWC 1 cut(s) 376
BsiSI CCGG 2 cut(s) 101, 182
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 2 cut(s) 100, 133
BsmAI GTCTC 3 cut(s) 160, 242, 328
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 4 cut(s) 63, 173, 248, 296
Bsp1286I GDGCHC 1 cut(s) 376
Bsp143I GATC 1 cut(s) 123
BspANI GGCC 4 cut(s) 63, 173, 248, 296
BspFNI CGCG 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 6, 91
BspPI GGATC 1 cut(s) 118
BspT107I GGYRCC 2 cut(s) 4, 89
BsrI ACTGG 2 cut(s) 205, 265
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 1 cut(s) 123
BssNI GRCGYC 2 cut(s) 90, 228
Bst2UI CCWGG 1 cut(s) 378
Bst4CI ACNGT 1 cut(s) 347
BstACI GRCGYC 2 cut(s) 90, 228
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 1 cut(s) 291
BstDSI CCRYGG 1 cut(s) 85
BstFNI CGCG 1 cut(s) 87
BstH2I RGCGCY 2 cut(s) 93, 264
BstHHI GCGC 2 cut(s) 92, 263
BstKTI GATC 1 cut(s) 126
BstMAI GTCTC 3 cut(s) 160, 242, 328
BstMBI GATC 1 cut(s) 123
BstMWI GCNNNNNNNGC 2 cut(s) 230, 335
BstNI CCWGG 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 376
BstUI CGCG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 182, 270
BsuRI GGCC 4 cut(s) 63, 173, 248, 296
BtgI CCRYGG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 1 cut(s) 316
CfoI GCGC 2 cut(s) 92, 263
Cfr42I CCGCGG 1 cut(s) 88
CseI GACGC 1 cut(s) 236
CviAII CATG 2 cut(s) 133, 298
CviJI RGCY 7 cut(s) 57, 63, 173, 181, 224, 248, 296
CviKI_1 RGCY 7 cut(s) 57, 63, 173, 181, 224, 248, 296
DdeI CTNAG 1 cut(s) 291
DinI GGCGCC 1 cut(s) 91
DpnI GATC 1 cut(s) 125
DpnII GATC 1 cut(s) 123
DriI GACNNNNNGTC 2 cut(s) 115, 145
EaeI YGGCCR 1 cut(s) 61
Eam1105I GACNNNNNGTC 2 cut(s) 115, 145
EciI GGCGGA 4 cut(s) 64, 152, 316, 325
Eco147I AGGCCT 1 cut(s) 248
EcoRII CCWGG 1 cut(s) 376
EgeI GGCGCC 1 cut(s) 91
EheI GGCGCC 1 cut(s) 91
FaeI CATG 2 cut(s) 136, 301
FaiI YATR 4 cut(s) 134, 215, 299, 383
FaqI GGGAC 1 cut(s) 286
FatI CATG 2 cut(s) 132, 297
FauI CCCGC 2 cut(s) 92, 316
Fnu4HI GCNGC 5 cut(s) 88, 196, 231, 259, 312
Fsp4HI GCNGC 5 cut(s) 88, 196, 231, 259, 312
FspBI CTAG 1 cut(s) 399
GlaI GCGC 2 cut(s) 91, 262
GluI GCNGC 5 cut(s) 88, 196, 231, 259, 312
HaeII RGCGCY 2 cut(s) 93, 264
HaeIII GGCC 4 cut(s) 63, 173, 248, 296
HapII CCGG 2 cut(s) 101, 182
HgaI GACGC 1 cut(s) 236
HhaI GCGC 2 cut(s) 92, 263
Hin1I GRCGYC 2 cut(s) 90, 228
Hin1II CATG 2 cut(s) 136, 301
Hin6I GCGC 2 cut(s) 90, 261
HinP1I GCGC 2 cut(s) 90, 261
HinfI GANTC 2 cut(s) 15, 204
HpaII CCGG 2 cut(s) 101, 182
Hpy166II GTNNAC 1 cut(s) 109
Hpy188III TCNNGA 5 cut(s) 12, 127, 208, 253, 280
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 121
HpyAV CCTTC 3 cut(s) 18, 179, 259
HpyCH4III ACNGT 1 cut(s) 347
HpyCH4V TGCA 2 cut(s) 198, 318
HpyF10VI GCNNNNNNNGC 2 cut(s) 230, 335
HpyF3I CTNAG 1 cut(s) 291
Hsp92I GRCGYC 2 cut(s) 90, 228
Hsp92II CATG 2 cut(s) 136, 301
HspAI GCGC 2 cut(s) 90, 261
KasI GGCGCC 1 cut(s) 89
KspI CCGCGG 1 cut(s) 88
Kzo9I GATC 1 cut(s) 123
LmnI GCTCC 3 cut(s) 42, 103, 379
Lsp1109I GCAGC 2 cut(s) 182, 270
MaeI CTAG 1 cut(s) 399
MalI GATC 1 cut(s) 125
MboI GATC 1 cut(s) 123
MboII GAAGA 2 cut(s) 379, 382
MhlI GDGCHC 1 cut(s) 376
Mly113I GGCGCC 1 cut(s) 90
MlyI GAGTC 2 cut(s) 9, 198
MmeI TCCRAC 1 cut(s) 183
MnlI CCTC 8 cut(s) 38, 50, 53, 88, 91, 238, 314, 343
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 87
MspI CCGG 2 cut(s) 101, 182
MspR9I CCNGG 1 cut(s) 378
MvaI CCWGG 1 cut(s) 378
MvnI CGCG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 230, 335
NarI GGCGCC 1 cut(s) 90
NdeII GATC 1 cut(s) 123
NlaIII CATG 2 cut(s) 136, 301
NlaIV GGNNCC 2 cut(s) 6, 91
NmeAIII GCCGAG 1 cut(s) 199
PceI AGGCCT 1 cut(s) 248
PkrI GCNGC 5 cut(s) 89, 197, 232, 260, 313
PleI GAGTC 2 cut(s) 9, 198
PluTI GGCGCC 1 cut(s) 93
PpsI GAGTC 2 cut(s) 9, 198
Psp6I CCWGG 1 cut(s) 376
PspGI CCWGG 1 cut(s) 376
PspN4I GGNNCC 2 cut(s) 6, 91
SacII CCGCGG 1 cut(s) 88
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 5 cut(s) 88, 196, 231, 259, 312
Sau3AI GATC 1 cut(s) 123
SchI GAGTC 2 cut(s) 9, 198
ScrFI CCNGG 1 cut(s) 378
SduI GDGCHC 1 cut(s) 376
SetI ASST 2 cut(s) 10, 226
SfoI GGCGCC 1 cut(s) 91
Sfr303I CCGCGG 1 cut(s) 88
SgrBI CCGCGG 1 cut(s) 88
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
SpeI ACTAGT 1 cut(s) 398
SseBI AGGCCT 1 cut(s) 248
SspDI GGCGCC 1 cut(s) 89
SspMI CTAG 1 cut(s) 399
StuI AGGCCT 1 cut(s) 248
StyD4I CCNGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 347
TauI GCSGC 3 cut(s) 90, 233, 314
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 1 cut(s) 205
TseI GCWGC 2 cut(s) 195, 258
TspRI CASTG 1 cut(s) 205
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.