Rmu_sc0002232.1_g000002

Microfibrillar-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002232.1
OG0017431
Physical Location & Seq
Reverse (-)
6415 .. 7596
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1182 bp
atgcgaggcaaaactagggaattgacaaaagttaaaagatactggcctggaaaagccccagagtgggcaaacgatcgtgatacctgctcctccgacgaagaagagattaccatcttgtacaaatccttcccaactcgtgatgatgatcctagacaacgtcgtttggccgagaggaggagtgtaaagaggcgcacccgccgtgccgagattgttgacacaactgaagaagaggaggaggaggaagaagaagaggacgcagagactttggaggaaagaagaagaaggattaaggagaggttgcttcgtcagatggagcaggaacaacaacaagaggagtttaagactgatgactactccacggaagacgaatccgatgaggactactcagaagaagaagaggatgagattgctggtgaagtagttacggtgaagcctgtatttttgtccaagtccacaagagacacgatagttgaacgtaagagacttgaggaggccgaagagcaggccctcgaagcgaggagaagggagctggaggagaggaagaaggagacaaggagaatggtgctcgaagagattcggaaagacgaagagattcataagggtttgatgaatcaggattcggcaaatgatgatgttgacactgacattaatgatcctgaggaggatactaaagcagcttggaaggcaagagagagggccaggctcaagaaggagagggcagcaagggaagtagtcattttgaaggagatagagaagatgaatgatcaagtgaggagggagtactgggagaggaagaatcctccctcatctcctgcgccacagccccgaaagaggaagcttaagttcatgcagagagatcaccacaggggagctttcttccattcggactctgatgatgaagatggcgttttaggaagagattattctaccccaaccggagaggataagatggataaaacaagactgcccaaggtgatgcaggtcaagaatttcgggcgtaggggaagggctaaatggactcatcttgtcaatgaggatactactgattggaacagcccgtggatgtggaatgatcgtcaaaggtcagggtataatgtgaaaatggcagccatgaatgcaccaatagcaagaccaaaaggaaacaagagattgaggttctctgtgaaggaagatgaactttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFAP1

Protein Analysis

393

Amino Acids

47.11

Weight (kDa)

5.39

Isoelectric Point (pI)

72.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFAP1 PF06991 132 - 347 1.9e-50 Microfibril-associated/Pre-mRNA processing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017431)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr3g0479581
rosa_laevigata RLG00000023558 RLG00000029558 RLG00000029584
rosa_multiflora Rmu_sc0002232.1_g000002 Rmu_sc0007129.1_g000020
rosa_roxburghii Rroxscaffold_6G00402140
rosa_rugosa Rorug03G0176600
rosa_samantha Rh3BG260500 Rh3CG257300 Rh3CG257900
rosa_wichuraiana Rw3G020310 Rw3G020360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 92, 970
AciI CCGC 1 cut(s) 196
AclWI GGATC 2 cut(s) 140, 647
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 1 cut(s) 988
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 119, 782
AfiI CCNNNNNNNGG 4 cut(s) 63, 64, 174, 831
AflII CTTAAG 1 cut(s) 839
AgsI TTSAA 2 cut(s) 473, 742
AjnI CCWGG 2 cut(s) 46, 698
AluBI AGCT 4 cut(s) 529, 677, 838, 872
AluI AGCT 4 cut(s) 529, 677, 838, 872
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 4 cut(s) 254, 453, 475, 542
AlwI GGATC 2 cut(s) 140, 647
AoxI GGCC 5 cut(s) 44, 165, 492, 504, 696
ApeKI GCWGC 3 cut(s) 674, 719, 1106
ApoI RAATTY 1 cut(s) 988
AseI ATTAAT 1 cut(s) 648
Asp700I GAANNNNTTC 2 cut(s) 573, 591
AspLEI GCGC 2 cut(s) 192, 817
AspS9I GGNCC 2 cut(s) 505, 696
AsuHPI GGTGA 4 cut(s) 425, 439, 851, 985
AxyI CCTNAGG 1 cut(s) 657
BarI GAAGNNNNNNTAC 2 cut(s) 110, 142
BauI CACGAG 1 cut(s) 135
BbsI GAAGAC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 3 cut(s) 686, 731, 1118
BccI CCATC 4 cut(s) 119, 304, 896, 943
BceAI ACGGC 1 cut(s) 183
BciT130I CCWGG 2 cut(s) 48, 700
BciVI GTATCC 2 cut(s) 658, 1030
BclI TGATCA 1 cut(s) 763
BcoDI GTCTC 4 cut(s) 254, 453, 475, 542
BfaI CTAG 2 cut(s) 15, 150
BfrI CTTAAG 1 cut(s) 839
BfuAI ACCTGC 2 cut(s) 92, 970
BfuI GTATCC 2 cut(s) 658, 1030
BisI GCNGC 3 cut(s) 675, 720, 1107
BlsI GCNGC 3 cut(s) 676, 721, 1108
BmcAI AGTACT 1 cut(s) 782
Bme1390I CCNGG 2 cut(s) 48, 700
BmgT120I GGNCC 2 cut(s) 505, 696
BmrFI CCNGG 2 cut(s) 48, 700
BmrI ACTGGG 1 cut(s) 793
BmsI GCATC 1 cut(s) 966
BmuI ACTGGG 1 cut(s) 793
BpiI GAAGAC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 368, 400
BpmI CTGGAG 1 cut(s) 551
BpuEI CTTGAG 2 cut(s) 506, 689
BsaBI GATNNNNATC 2 cut(s) 110, 144
BsaJI CCNNGG 3 cut(s) 357, 969, 1058
BsaWI WCCGGW 1 cut(s) 935
Bsc4I CCNNNNNNNGG 4 cut(s) 63, 64, 174, 831
Bse1I ACTGG 2 cut(s) 47, 788
Bse21I CCTNAGG 1 cut(s) 657
Bse8I GATNNNNATC 2 cut(s) 110, 144
BseBI CCWGG 2 cut(s) 48, 700
BseDI CCNNGG 3 cut(s) 357, 969, 1058
BseGI GGATG 2 cut(s) 406, 1068
BseJI GATNNNNATC 2 cut(s) 110, 144
BseLI CCNNNNNNNGG 4 cut(s) 63, 64, 174, 831
BseMII CTCAG 2 cut(s) 399, 648
BseNI ACTGG 2 cut(s) 47, 788
BseXI GCAGC 3 cut(s) 686, 731, 1118
Bsh1285I CGRYCG 1 cut(s) 76
BshFI GGCC 5 cut(s) 46, 167, 494, 506, 698
BsiEI CGRYCG 1 cut(s) 76
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 936
BslI CCNNNNNNNGG 4 cut(s) 63, 64, 174, 831
BsmAI GTCTC 4 cut(s) 254, 453, 475, 542
BsmI GAATGC 1 cut(s) 1120
BsnI GGCC 5 cut(s) 46, 167, 494, 506, 698
Bsp1286I GDGCHC 1 cut(s) 567
Bsp1407I TGTACA 1 cut(s) 117
Bsp143I GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
BspACI CCGC 1 cut(s) 196
BspANI GGCC 5 cut(s) 46, 167, 494, 506, 698
BspCNI CTCAG 2 cut(s) 398, 649
BspMI ACCTGC 2 cut(s) 92, 970
BspPI GGATC 2 cut(s) 140, 647
BspQI GCTCTTC 1 cut(s) 492
BspTI CTTAAG 1 cut(s) 839
BsrGI TGTACA 1 cut(s) 117
BsrI ACTGG 2 cut(s) 47, 788
BssECI CCNNGG 3 cut(s) 357, 969, 1058
BssMI GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
BssSI CACGAG 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 969
Bst2BI CACGAG 1 cut(s) 135
Bst2UI CCWGG 2 cut(s) 48, 700
Bst4CI ACNGT 1 cut(s) 427
Bst6I CTCTTC 8 cut(s) 96, 222, 243, 390, 492, 564, 582, 910
BstAFI CTTAAG 1 cut(s) 839
BstAUI TGTACA 1 cut(s) 117
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 2 cut(s) 385, 657
BstDSI CCRYGG 2 cut(s) 357, 1058
BstF5I GGATG 2 cut(s) 406, 1068
BstHHI GCGC 2 cut(s) 192, 817
BstKTI GATC 6 cut(s) 76, 148, 655, 766, 859, 1075
BstMAI GTCTC 4 cut(s) 254, 453, 475, 542
BstMBI GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
BstMCI CGRYCG 1 cut(s) 76
BstMWI GCNNNNNNNGC 4 cut(s) 512, 683, 1115, 1124
BstNI CCWGG 2 cut(s) 48, 700
BstSCI CCNGG 2 cut(s) 46, 698
BstV1I GCAGC 3 cut(s) 686, 731, 1118
BstV2I GAAGAC 1 cut(s) 369
Bsu36I CCTNAGG 1 cut(s) 657
BsuI GTATCC 2 cut(s) 658, 1030
BsuRI GGCC 5 cut(s) 46, 167, 494, 506, 698
BtgI CCRYGG 2 cut(s) 357, 1058
BtsCI GGATG 2 cut(s) 406, 1068
BtsIMutI CAGTG 1 cut(s) 639
BveI ACCTGC 2 cut(s) 92, 970
Cac8I GCNNGC 1 cut(s) 504
CfoI GCGC 2 cut(s) 192, 817
Cfr13I GGNCC 2 cut(s) 505, 696
CseI GACGC 1 cut(s) 263
Csp6I GTAC 2 cut(s) 118, 781
CviAII CATG 2 cut(s) 847, 1111
CviQI GTAC 2 cut(s) 118, 781
DdeI CTNAG 2 cut(s) 385, 657
DpnI GATC 6 cut(s) 75, 147, 654, 765, 858, 1074
DpnII GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
EaeI YGGCCR 1 cut(s) 165
Eam1104I CTCTTC 8 cut(s) 96, 222, 243, 390, 492, 564, 582, 910
EarI CTCTTC 8 cut(s) 96, 222, 243, 390, 492, 564, 582, 910
Eco130I CCWWGG 1 cut(s) 969
Eco57I CTGAAG 1 cut(s) 243
Eco81I CCTNAGG 1 cut(s) 657
EcoO109I RGGNCCY 1 cut(s) 505
EcoRII CCWGG 2 cut(s) 46, 698
EcoT14I CCWWGG 1 cut(s) 969
ErhI CCWWGG 1 cut(s) 969
FaeI CATG 2 cut(s) 850, 1114
FaiI YATR 4 cut(s) 597, 848, 1092, 1112
FalI AAGNNNNNCTT 1 cut(s) 1161
FatI CATG 2 cut(s) 846, 1110
FauI CCCGC 1 cut(s) 203
FbaI TGATCA 1 cut(s) 763
Fnu4HI GCNGC 3 cut(s) 675, 720, 1107
FokI GGATG 2 cut(s) 413, 1075
Fsp4HI GCNGC 3 cut(s) 675, 720, 1107
FspBI CTAG 2 cut(s) 15, 150
GlaI GCGC 2 cut(s) 191, 816
GluI GCNGC 3 cut(s) 675, 720, 1107
GsuI CTGGAG 1 cut(s) 551
HaeIII GGCC 5 cut(s) 46, 167, 494, 506, 698
HapII CCGG 1 cut(s) 936
HgaI GACGC 1 cut(s) 263
HhaI GCGC 2 cut(s) 192, 817
Hin1II CATG 2 cut(s) 850, 1114
Hin6I GCGC 2 cut(s) 190, 815
HinP1I GCGC 2 cut(s) 190, 815
HincII GTYRAC 2 cut(s) 214, 637
HindII GTYRAC 2 cut(s) 214, 637
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 8 cut(s) 368, 574, 592, 610, 617, 796, 887, 1018
HpaII CCGG 1 cut(s) 936
HphI GGTGA 4 cut(s) 425, 439, 851, 985
Hpy166II GTNNAC 3 cut(s) 214, 453, 637
Hpy188I TCNGA 7 cut(s) 94, 309, 373, 388, 579, 886, 892
Hpy188III TCNNGA 6 cut(s) 77, 137, 614, 656, 706, 985
Hpy8I GTNNAC 3 cut(s) 214, 453, 637
Hpy99I CGWCG 2 cut(s) 98, 162
HpyAV CCTTC 9 cut(s) 136, 276, 516, 538, 676, 703, 736, 999, 1159
HpyCH4III ACNGT 1 cut(s) 427
HpyCH4IV ACGT 2 cut(s) 157, 475
HpyCH4V TGCA 3 cut(s) 850, 979, 1118
HpyF10VI GCNNNNNNNGC 4 cut(s) 512, 683, 1115, 1124
HpyF3I CTNAG 2 cut(s) 385, 657
HpySE526I ACGT 2 cut(s) 157, 475
Hsp92II CATG 2 cut(s) 850, 1114
HspAI GCGC 2 cut(s) 190, 815
Ksp22I TGATCA 1 cut(s) 763
Kzo9I GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 4 cut(s) 92, 313, 526, 869
Lsp1109I GCAGC 3 cut(s) 686, 731, 1118
LweI GCATC 1 cut(s) 966
MaeI CTAG 2 cut(s) 15, 150
MaeII ACGT 2 cut(s) 157, 475
MaeIII GTNAC 1 cut(s) 421
MalI GATC 6 cut(s) 75, 147, 654, 765, 858, 1074
MboI GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 2 cut(s) 20, 988
MlyI GAGTC 2 cut(s) 881, 1012
MmeI TCCRAC 1 cut(s) 117
MroXI GAANNNNTTC 2 cut(s) 573, 591
MseI TTAA 6 cut(s) 33, 288, 339, 648, 840, 1180
MspCI CTTAAG 1 cut(s) 839
MspI CCGG 1 cut(s) 936
MspR9I CCNGG 2 cut(s) 48, 700
Mva1269I GAATGC 1 cut(s) 1120
MvaI CCWGG 2 cut(s) 48, 700
MwoI GCNNNNNNNGC 4 cut(s) 512, 683, 1115, 1124
NdeII GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
NlaIII CATG 2 cut(s) 850, 1114
NmeAIII GCCGAG 2 cut(s) 193, 229
PciSI GCTCTTC 1 cut(s) 492
PctI GAATGC 1 cut(s) 1120
PdmI GAANNNNTTC 2 cut(s) 573, 591
PfeI GAWTC 6 cut(s) 368, 574, 592, 610, 617, 796
PflFI GACNNNGTC 1 cut(s) 156
PkrI GCNGC 3 cut(s) 676, 721, 1108
Ple19I CGATCG 1 cut(s) 76
PleI GAGTC 2 cut(s) 881, 1012
PpsI GAGTC 2 cut(s) 881, 1012
PshBI ATTAAT 1 cut(s) 648
Psp6I CCWGG 2 cut(s) 46, 698
PspGI CCWGG 2 cut(s) 46, 698
PspPI GGNCC 2 cut(s) 505, 696
PsyI GACNNNGTC 1 cut(s) 156
PvuI CGATCG 1 cut(s) 76
RsaI GTAC 2 cut(s) 119, 782
RsaNI GTAC 2 cut(s) 118, 781
SapI GCTCTTC 1 cut(s) 492
SaqAI TTAA 6 cut(s) 33, 288, 339, 648, 840, 1180
SatI GCNGC 3 cut(s) 675, 720, 1107
Sau3AI GATC 6 cut(s) 73, 145, 652, 763, 856, 1072
Sau96I GGNCC 2 cut(s) 505, 696
ScaI AGTACT 1 cut(s) 782
SchI GAGTC 2 cut(s) 881, 1012
ScrFI CCNGG 2 cut(s) 48, 700
SduI GDGCHC 1 cut(s) 567
SfaNI GCATC 1 cut(s) 966
SmlI CTYRAG 3 cut(s) 485, 704, 839
SmoI CTYRAG 3 cut(s) 485, 704, 839
Sse9I AATT 2 cut(s) 20, 988
SsiI CCGC 1 cut(s) 196
SspMI CTAG 2 cut(s) 15, 150
StyD4I CCNGG 2 cut(s) 46, 698
StyI CCWWGG 1 cut(s) 969
TaaI ACNGT 1 cut(s) 427
TaiI ACGT 2 cut(s) 160, 478
TaqI TCGA 2 cut(s) 510, 567
TasI AATT 2 cut(s) 20, 988
TatI WGTACW 2 cut(s) 117, 780
TfiI GAWTC 6 cut(s) 368, 574, 592, 610, 617, 796
Tru1I TTAA 6 cut(s) 33, 288, 339, 648, 840, 1180
Tru9I TTAA 6 cut(s) 33, 288, 339, 648, 840, 1180
TscAI CASTG 1 cut(s) 646
TseI GCWGC 3 cut(s) 674, 719, 1106
TspDTI ATGAA 6 cut(s) 584, 623, 773, 835, 912, 1127
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 1 cut(s) 646
Tth111I GACNNNGTC 1 cut(s) 156
Vha464I CTTAAG 1 cut(s) 839
VspI ATTAAT 1 cut(s) 648
XapI RAATTY 1 cut(s) 988
XmnI GAANNNNTTC 2 cut(s) 573, 591
XspI CTAG 2 cut(s) 15, 150
ZrmI AGTACT 1 cut(s) 782
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.