Rmu_sc0002239.1_g000010

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002239.1
OG0016644
Physical Location & Seq
Reverse (-)
40121 .. 46746
6626 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4056 bp
atggtcggaaagggagagggaccggcgatcggaatcgatctcggaactacctacagttgcgtcggagtgtggcagcacgaccgcgtcgagatcatcgccaacgatcagggcaacagaacgacgccgtcttacgttgcgttcactgatactgagcggttgatcggcgatgcggccaagaaccaggtcgccatgaaccctgtcaacaccgttttcgatgcgaagcggttaattggtaggagatttggtgactcctctgttcaggcagacatgaagctatggcctttcaaggttgttgccggccctggtgacaagcccatgattattgttacttacaaaggcgaggagaaacaatttgctgcagaagaaatctcgtctatggttctcgtcaagatgcgagagattgcagaggcttatcttgggacaacagtgaagaatgctgttgttaccgtacctgcttacttcaatgactctcagaggcaggctacaaaggatgctggggtcattgctggcctgaatgtgctccgtattatcaatgagcctactgctgctgccattgcgtatggtctggacaagaaggcgaccagtgttggcgagaagaatgtcttgatttttgatcttggaggaggaacctttgatgtttctcttcttacaattgaagaaggtattttcgaggtgaaggctactgctggagatacccatttgggtggtgaggattttgataacaggatggtgaatcactttgttcaggagttcaagaggaagaacaaaaaagacatcagcggaaacccaagagctctaaggaggttgaggacagcttgtgagagagcaaagaggactctctcatccactgctcagaccaccatcgagattgactctctgtttgagggtatcgacttctattcaaccatcacccgtgccagattcgaagagctgaacatggatctgttcaggaagtgtatggagccagttgagaagtgtttgagagatgcaaagatggacaagagcagtgtacatgatgttgtccttgtgggtggatctactaggattcctaaagtgcagcaattgttgcaagacttcttcaatggaaaggaactgtgcaaaagcatcaacccagacgaagctgttgcttatggagcagctgtgcaggctgctattcttagtggtgagggtaatgagaaggtgcaggacctattgcttcttgatgtgactcctctgtccttgggtttggaaactgccggaggtgtgatgactgtgctcatccccaggaatacaacaattccaaccaagaaggagcaggttttctctacctactcggacaaccaacccggtgtcttgatccaggtctttgagggtgagagggcgagaacccgtgataacaatttgcttggcaagtttgagctttccggaattcctcctgcaccaaggggagtacctcagatcacggtctgctttgacatcgatgctaatggcattctcaatgtctctgctgaggacaagactacaggccagaagaacaagattacaatcactaatgacaagggaaggctgtccaaagaggagattgagaagatggttcaagaagctgagaagtataagtcagaagatgaggagctcaagaagaaggtggaatcaaagaatgccctggagaactatgcttataacatgaggaacacgttcgagcaacgagtctcaacccatttcatccatagcattgtggatgcaacccagtccaagttcgactccaatctactactaaagcggttatctgtttctggtcgaatcgatgatgcccgccaagtgttcgatgaaatgccgagcagagatgagttcacttggaatactatgatagctgcttatgccaattcaggaagactaaccgaagctaaacaagtctttgatgctaccccaagtaagactgccattacttggtcctcactcatatctgggtattgtcgaaatgggtgtcaaagcgaagcttttgaatcgttttggcaaatgcagcttgggggagaaaggccgagccagtacacattgggcagtgtactgaggttgtgtgctgcattgggtttgatccaaagaggtgaaatggtgcatggatatatggtgaaaacccagtttgacagtaatgttgatgtagttactggtttggttgacatgtatgcaaagtgcaagcgcgtttttgaagctgaatgtgtatttgagagtttgtgggataggagaaatcatgtcgtgtggactgtcatgcttaccgggtactctcagaatggggatggcttgaaggcattgaagtgttttcgggatatgcgagcacgaggggttgaatccaatcagttcacattccctagcgtattgactgctgctgcgtcggttttagcccttggttttggggctcaggtgcatggatgcattattcagactggttttgggcctaatgtgtttgttcaaagtgcattggttgacatgtacgtgaaatgtggagatcatatcagtgcaaagaaggcactaagaagcatggaggttgacgatgttgtttcttggaactcgatgattgttggatgtgtaaggcaggggtttatacgagaagctttatcgttgtttagagaaatgcgttcaagagaaatgaagattgatgattatacatatccatcagttctgaactcatttgctgcactgaaggacgttaaaaatgcaatggcggttcattgtctgatagttaagactggatttgaggtttatcaacttgtagccaatgctctggttgatatgtatgctaaacagggaaatatagaatctgcacttgagatgtttaggcatattccagacaaagatgttatctcttggacctctctggtaacaggctatgcacagaatggcactcatgaaaaagcacttaaattattctgcgagatgagggatgcagggatctgtcctgaccaatttgtcattgccagcattttgagtgcctgtgcagaactgacacttttggagtttggccaacaaattcatgcaaatttcatcaagtctggccttcaagcatcattatcggtagataattcctttgtaacgatgtatgcaaagtgtgggtgtctagaagaggctaatagagtttttgattctatgcaagttcaaaatgtgattacttggacagctttgatagttggttatgcccagaatggtagaggaaaggagtctctaaaattctacgatcaaatgcttgcaactggcacccaaccagacttcattacttttattggcttgttatttgcttgtagccatgctggtcttcaagaaaaaggtcagtactactttgaatcgatgaatagggtttatggaataaaaccaggtcctgagcactatgcatgtatgattgacctcttggggcgctcagggaggcttaatgaggctgaggcattagttaatcaaatggctgtagaacctgatgggactgtatggaaggcactacttgctgcatgtagagtacatgggaaaatagagctgggagagagggctgcagagaacctatttatattggagcctatgaatgctgtgccttatgttcagttatctaacatgtattctgcagctggtagatgggaagatgcggcaagaatcagaagactgatgaaatcaaagggaattcttaaggagcctggatgtagttggattgagatgaacggccaagttcatacctttatgtctgaagacagaagccattcatgcacaactgaaatatattcaaaaattgatgagatcatgatgcttacaaaggaagctggatacatgccagatatgaattttgcactacatgacatggataaagaggggaaggagcttggcctagcatatcacagcgagaagttggccattgcttttggccttctcaccacaccgccaggggcaccaattcgagtttttaagaatctccgggtttgtggagattgtcatactgcaatgaaatatatatcacgggtttatcttcgtcatattatattgagggattcaaattgttttcatcattttaaagaagggaagtgttcttgtgatgactactggtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR

Protein Analysis

1351

Amino Acids

150.26

Weight (kDa)

6.11

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 9 - 565 1.3e-253 Hsp70 protein
MreB_Mbl PF06723 141 - 380 1.5e-12 MreB/Mbl protein
PPR_2 PF13041 639 - 685 5.7e-06 PPR repeat family
PPR_2 PF13041 741 - 786 2.5e-09 PPR repeat family
PPR PF01535 743 - 773 1.3e-06 PPR repeat
PPR_2 PF13041 842 - 889 7.9e-12 PPR repeat family
PPR PF01535 844 - 872 2.9e-08 PPR repeat
PPR_2 PF13041 942 - 990 1e-09 PPR repeat family
PPR PF01535 945 - 975 1.5e-07 PPR repeat
PPR_2 PF13041 1044 - 1091 1.8e-08 PPR repeat family
E_motif PF20431 1161 - 1223 1e-19 E motif
DYW_deaminase PF14432 1259 - 1351 4e-40 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016644)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61170
fragaria_vesca FvH4_5g34470
malus_domestica MD07G1126900.v1.1
prunus_persica Prupe.2G157100_v2.0.a1
pyrus_communis pycom07g12240
rosa_chinensis RchiOBHm_Chr7g0235091
rosa_multiflora Rmu_sc0002239.1_g000010
rosa_roxburghii Rroxscaffold_3G00226310
rosa_rugosa Rorug07G0287300 Rorug07G0287400
rosa_samantha Rh7AG442400 Rh7BG415000 Rh7CG463300
rosa_wichuraiana Rw7G036780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1659
AasI GACNNNNNNGTC 2 cut(s) 1213, 2939
Acc36I ACCTGC 2 cut(s) 460, 1285
AccB1I GGYRCC 2 cut(s) 3224, 3898
AccB7I CCANNNNNTGG 1 cut(s) 1926
AccBSI CCGCTC 1 cut(s) 154
AccII CGCG 2 cut(s) 84, 2175
AccIII TCCGGA 1 cut(s) 1403
AclWI GGATC 5 cut(s) 948, 1042, 1330, 2065, 2930
AcoI YGGCCR 4 cut(s) 171, 2992, 3676, 3861
AcsI RAATTY 6 cut(s) 1407, 3000, 3010, 3197, 3636, 3793
AcuI CTGAAG 2 cut(s) 2693, 3720
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 9 cut(s) 450, 1011, 1431, 2027, 2043, 2255, 2465, 3302, 3480
AfiI CCNNNNNNNGG 2 cut(s) 29, 1926
AflII CTTAAG 1 cut(s) 3641
AflIII ACRYGT 4 cut(s) 1671, 2154, 2460, 3570
AjnI CCWGG 8 cut(s) 180, 301, 1262, 1338, 1641, 3340, 3649, 3892
Alw21I GWGCWC 6 cut(s) 522, 796, 1257, 1614, 2311, 3354
Alw26I GTCTC 3 cut(s) 1486, 1693, 3195
AlwI GGATC 5 cut(s) 948, 1042, 1330, 2065, 2930
AlwNI CAGNNNCTG 1 cut(s) 3347
Aor13HI TCCGGA 1 cut(s) 1403
ApoI RAATTY 6 cut(s) 1407, 3000, 3010, 3197, 3636, 3793
ArsI GACNNNNNNTTYG 4 cut(s) 863, 895, 1948, 1980
Asp700I GAANNNNTTC 2 cut(s) 1673, 3712
AspLEI GCGC 2 cut(s) 2175, 3384
AspS9I GGNCC 7 cut(s) 20, 299, 1186, 1929, 2426, 2841, 3344
AsuC2I CCSGG 3 cut(s) 1326, 2251, 3926
AsuII TTCGAA 1 cut(s) 924
AvaII GGWCC 5 cut(s) 20, 1186, 1929, 2841, 3344
BaeGI GKGCMC 1 cut(s) 3901
BalI TGGCCA 2 cut(s) 2994, 3863
BanI GGYRCC 2 cut(s) 3224, 3898
BanII GRGCYC 3 cut(s) 796, 1614, 2392
BauI CACGAG 1 cut(s) 2310
BbsI GAAGAC 4 cut(s) 1876, 3275, 3622, 3708
Bbv12I GWGCWC 6 cut(s) 522, 796, 1257, 1614, 2311, 3354
BbvCI CCTCAGC 2 cut(s) 1488, 3405
BccI CCATC 9 cut(s) 721, 869, 914, 988, 1564, 2264, 2653, 3434, 3585
BceAI ACGGC 2 cut(s) 109, 3691
BcgI CGANNNNNNTGC 4 cut(s) 1106, 1140, 3024, 3058
BciT130I CCWGG 8 cut(s) 182, 303, 1264, 1340, 1643, 3342, 3651, 3894
BciVI GTATCC 1 cut(s) 3770
BcnI CCSGG 3 cut(s) 1326, 2251, 3926
BcoDI GTCTC 3 cut(s) 1486, 1693, 3195
BfaI CTAG 4 cut(s) 1041, 2343, 3089, 3839
BfmI CTRYAG 6 cut(s) 52, 357, 1500, 3429, 3510, 3579
BfoI RGCGCY 1 cut(s) 3385
BfrI CTTAAG 1 cut(s) 3641
BfuAI ACCTGC 2 cut(s) 460, 1285
BfuI GTATCC 1 cut(s) 3770
BmcAI AGTACT 1 cut(s) 3302
Bme18I GGWCC 5 cut(s) 20, 1186, 1929, 2841, 3344
BmgT120I GGNCC 7 cut(s) 20, 299, 1186, 1929, 2426, 2841, 3344
BmiI GGNNCC 7 cut(s) 21, 628, 963, 3226, 3534, 3648, 3900
BmrI ACTGGG 2 cut(s) 1720, 2107
BmuI ACTGGG 2 cut(s) 1720, 2107
BpiI GAAGAC 4 cut(s) 1876, 3275, 3622, 3708
BplI GAGNNNNNCTC 2 cut(s) 857, 889
BpmI CTGGAG 2 cut(s) 708, 1664
Bpu10I CCTNAGC 5 cut(s) 1488, 2391, 3348, 3385, 3405
Bpu14I TTCGAA 1 cut(s) 924
BpuEI CTTGAG 2 cut(s) 1598, 2819
BpuMI CCSGG 3 cut(s) 1326, 2251, 3926
Bsa29I ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BsaAI YACGTR 1 cut(s) 2467
BsaBI GATNNNNATC 2 cut(s) 32, 1523
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 7 cut(s) 301, 1218, 1262, 1421, 1641, 2377, 3893
BsaWI WCCGGW 1 cut(s) 1403
BsaXI ACNNNNNCTCC 4 cut(s) 229, 259, 1125, 1155
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 1926
Bse118I RCCGGY 2 cut(s) 22, 296
Bse3DI GCAATG 6 cut(s) 501, 552, 2697, 2943, 3864, 3957
Bse8I GATNNNNATC 2 cut(s) 32, 1523
BseAI TCCGGA 1 cut(s) 1403
BseBI CCWGG 8 cut(s) 182, 303, 1264, 1340, 1643, 3342, 3651, 3894
BseCI ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BseDI CCNNGG 7 cut(s) 301, 1218, 1262, 1421, 1641, 2377, 3893
BseJI GATNNNNATC 2 cut(s) 32, 1523
BseLI CCNNNNNNNGG 2 cut(s) 29, 1926
BseMI GCAATG 6 cut(s) 501, 552, 2697, 2943, 3864, 3957
BseRI GAGGAG 6 cut(s) 241, 356, 636, 1200, 1571, 1622
BseSI GKGCMC 1 cut(s) 3901
BseYI CCCAGC 2 cut(s) 494, 3496
BsgI GTGCAG 7 cut(s) 1076, 1163, 1202, 1401, 2652, 2778, 2988
Bsh1236I CGCG 2 cut(s) 84, 2175
Bsh1285I CGRYCG 2 cut(s) 30, 82
BshNI GGYRCC 2 cut(s) 3224, 3898
BshVI ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BsiEI CGRYCG 2 cut(s) 30, 82
BsiHKAI GWGCWC 6 cut(s) 522, 796, 1257, 1614, 2311, 3354
BsiSI CCGG 7 cut(s) 23, 297, 1236, 1326, 1404, 2250, 3925
BslFI GGGAC 3 cut(s) 33, 433, 3457
BslI CCNNNNNNNGG 2 cut(s) 29, 1926
BsmAI GTCTC 3 cut(s) 1486, 1693, 3195
BsmFI GGGAC 3 cut(s) 33, 433, 3457
BsmI GAATGC 4 cut(s) 439, 1470, 1642, 3547
Bsp119I TTCGAA 1 cut(s) 924
Bsp1286I GDGCHC 8 cut(s) 522, 796, 1257, 1614, 2311, 2392, 3354, 3901
Bsp13I TCCGGA 1 cut(s) 1403
Bsp1407I TGTACA 1 cut(s) 1009
BspDI ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BspEI TCCGGA 1 cut(s) 1403
BspFNI CGCG 2 cut(s) 84, 2175
BspHI TCATGA 2 cut(s) 2878, 3753
BspLI GGNNCC 7 cut(s) 21, 628, 963, 3226, 3534, 3648, 3900
BspMAI CTGCAG 3 cut(s) 361, 3514, 3583
BspMI ACCTGC 2 cut(s) 460, 1285
BspPI GGATC 5 cut(s) 948, 1042, 1330, 2065, 2930
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 924
BspT107I GGYRCC 2 cut(s) 3224, 3898
BspTI CTTAAG 1 cut(s) 3641
BsrBI CCGCTC 1 cut(s) 154
BsrDI GCAATG 6 cut(s) 501, 552, 2697, 2943, 3864, 3957
BsrFI RCCGGY 2 cut(s) 22, 296
BsrGI TGTACA 1 cut(s) 1009
BssAI RCCGGY 2 cut(s) 22, 296
BssECI CCNNGG 7 cut(s) 301, 1218, 1262, 1421, 1641, 2377, 3893
BssNI GRCGYC 1 cut(s) 122
BssSI CACGAG 1 cut(s) 2310
BssT1I CCWWGG 3 cut(s) 1218, 1421, 2377
Bst2BI CACGAG 1 cut(s) 2310
Bst2UI CCWGG 8 cut(s) 182, 303, 1264, 1340, 1643, 3342, 3651, 3894
Bst6I CTCTTC 3 cut(s) 648, 921, 3087
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 3641
BstAPI GCANNNNNTGC 2 cut(s) 1066, 3464
BstAUI TGTACA 1 cut(s) 1009
BstBAI YACGTR 1 cut(s) 2467
BstBI TTCGAA 1 cut(s) 924
BstC8I GCNNGC 9 cut(s) 298, 480, 508, 1146, 1792, 2171, 2307, 2950, 3216
BstFNI CGCG 2 cut(s) 84, 2175
BstH2I RGCGCY 1 cut(s) 3385
BstHHI GCGC 2 cut(s) 2175, 3384
BstMAI GTCTC 3 cut(s) 1486, 1693, 3195
BstMCI CGRYCG 2 cut(s) 30, 82
BstMWI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1856, 1999, 2498, 3464, 3717
BstNI CCWGG 8 cut(s) 182, 303, 1264, 1340, 1643, 3342, 3651, 3894
BstNSI RCATGY 6 cut(s) 2158, 2464, 3363, 3474, 3574, 3784
BstSFI CTRYAG 6 cut(s) 52, 357, 1500, 3429, 3510, 3579
BstSLI GKGCMC 1 cut(s) 3901
BstUI CGCG 2 cut(s) 84, 2175
BstV2I GAAGAC 4 cut(s) 1876, 3275, 3622, 3708
BstX2I RGATCY 3 cut(s) 940, 1034, 2922
BstXI CCANNNNNNTGG 2 cut(s) 704, 2755
BstYI RGATCY 3 cut(s) 940, 1034, 2922
Bsu15I ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BsuI GTATCC 1 cut(s) 3770
BsuTUI ATCGAT 4 cut(s) 36, 1458, 1782, 3314
BtgZI GCGATG 2 cut(s) 79, 180
BtsI GCAGTG 3 cut(s) 846, 1012, 2044
BtsIMutI CAGTG 8 cut(s) 141, 432, 589, 846, 1012, 2044, 2494, 2669
BveI ACCTGC 2 cut(s) 460, 1285
Cac8I GCNNGC 9 cut(s) 298, 480, 508, 1146, 1792, 2171, 2307, 2950, 3216
CaiI CAGNNNCTG 1 cut(s) 3347
CciI TCATGA 2 cut(s) 2878, 3753
CfoI GCGC 2 cut(s) 2175, 3384
Cfr10I RCCGGY 2 cut(s) 22, 296
Cfr13I GGNCC 7 cut(s) 20, 299, 1186, 1929, 2426, 2841, 3344
ClaI ATCGAT 4 cut(s) 36, 1458, 1782, 3314
CseI GACGC 4 cut(s) 49, 73, 130, 2352
CsiI ACCWGGT 2 cut(s) 180, 3340
Csp6I GTAC 9 cut(s) 449, 1010, 1430, 2026, 2042, 2254, 2464, 3301, 3479
CviQI GTAC 9 cut(s) 449, 1010, 1430, 2026, 2042, 2254, 2464, 3301, 3479
DraI TTTAAA 1 cut(s) 4021
DrdI GACNNNNNNGTC 2 cut(s) 1213, 2939
DseDI GACNNNNNNGTC 2 cut(s) 1213, 2939
EaeI YGGCCR 4 cut(s) 171, 2992, 3676, 3861
Eam1104I CTCTTC 3 cut(s) 648, 921, 3087
EarI CTCTTC 3 cut(s) 648, 921, 3087
Ecl136II GAGCTC 2 cut(s) 794, 1612
Eco130I CCWWGG 3 cut(s) 1218, 1421, 2377
Eco24I GRGCYC 3 cut(s) 796, 1614, 2392
Eco47I GGWCC 5 cut(s) 20, 1186, 1929, 2841, 3344
Eco53kI GAGCTC 2 cut(s) 794, 1612
Eco57I CTGAAG 2 cut(s) 2693, 3720
EcoICRI GAGCTC 2 cut(s) 794, 1612
EcoO109I RGGNCCY 2 cut(s) 1186, 3344
EcoRI GAATTC 2 cut(s) 1407, 3636
EcoRII CCWGG 8 cut(s) 180, 301, 1262, 1338, 1641, 3340, 3649, 3892
EcoT14I CCWWGG 3 cut(s) 1218, 1421, 2377
EcoT22I ATGCAT 2 cut(s) 2408, 3361
EcoT38I GRGCYC 3 cut(s) 796, 1614, 2392
ErhI CCWWGG 3 cut(s) 1218, 1421, 2377
FalI AAGNNNNNCTT 4 cut(s) 587, 619, 1960, 1992
FaqI GGGAC 3 cut(s) 33, 433, 3457
FauI CCCGC 1 cut(s) 1799
FriOI GRGCYC 3 cut(s) 796, 1614, 2392
FspBI CTAG 4 cut(s) 1041, 2343, 3089, 3839
GlaI GCGC 2 cut(s) 2174, 3383
GsaI CCCAGC 2 cut(s) 498, 3500
GsuI CTGGAG 2 cut(s) 708, 1664
HaeII RGCGCY 1 cut(s) 3385
HapII CCGG 7 cut(s) 23, 297, 1236, 1326, 1404, 2250, 3925
HgaI GACGC 4 cut(s) 49, 73, 130, 2352
HhaI GCGC 2 cut(s) 2175, 3384
Hin1I GRCGYC 1 cut(s) 122
Hin6I GCGC 2 cut(s) 2173, 3382
HinP1I GCGC 2 cut(s) 2173, 3382
HincII GTYRAC 4 cut(s) 202, 2152, 2458, 2521
HindII GTYRAC 4 cut(s) 202, 2152, 2458, 2521
HindIII AAGCTT 2 cut(s) 1974, 2583
HpaII CCGG 7 cut(s) 23, 297, 1236, 1326, 1404, 2250, 3925
Hpy99I CGWCG 4 cut(s) 65, 89, 124, 2368
HpyCH4IV ACGT 4 cut(s) 132, 1673, 2466, 2679
HpyF10VI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1856, 1999, 2498, 3464, 3717
HpySE526I ACGT 4 cut(s) 132, 1673, 2466, 2679
Hsp92I GRCGYC 1 cut(s) 122
HspAI GCGC 2 cut(s) 2173, 3382
Kpn2I TCCGGA 1 cut(s) 1403
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 8 cut(s) 525, 961, 1133, 1291, 1609, 3532, 3646, 3829
MabI ACCWGGT 2 cut(s) 180, 3340
MaeI CTAG 4 cut(s) 1041, 2343, 3089, 3839
MaeII ACGT 4 cut(s) 132, 1673, 2466, 2679
MaeIII GTNAC 8 cut(s) 245, 305, 325, 442, 1204, 2137, 2851, 3061
MbiI CCGCTC 1 cut(s) 154
MfeI CAATTG 2 cut(s) 651, 1061
MflI RGATCY 3 cut(s) 940, 1034, 2922
MhlI GDGCHC 8 cut(s) 522, 796, 1257, 1614, 2311, 2392, 3354, 3901
MlsI TGGCCA 2 cut(s) 2994, 3863
MluNI TGGCCA 2 cut(s) 2994, 3863
MlyI GAGTC 8 cut(s) 242, 461, 829, 866, 1201, 1695, 1733, 3197
MmeI TCCRAC 4 cut(s) 43, 1304, 2533, 3641
Mox20I TGGCCA 2 cut(s) 2994, 3863
Mph1103I ATGCAT 2 cut(s) 2408, 3361
MroI TCCGGA 1 cut(s) 1403
MroNI GCCGGC 1 cut(s) 296
MroXI GAANNNNTTC 2 cut(s) 1673, 3712
MscI TGGCCA 2 cut(s) 2994, 3863
MseI TTAA 9 cut(s) 227, 2682, 2715, 2892, 3396, 3417, 3642, 3915, 4020
MslI CAYNNNNRTG 4 cut(s) 702, 2287, 2465, 2487
Msp20I TGGCCA 2 cut(s) 2994, 3863
MspA1I CMGCKG 3 cut(s) 780, 1139, 3584
MspCI CTTAAG 1 cut(s) 3641
MspI CCGG 7 cut(s) 23, 297, 1236, 1326, 1404, 2250, 3925
MunI CAATTG 2 cut(s) 651, 1061
Mva1269I GAATGC 4 cut(s) 439, 1470, 1642, 3547
MvaI CCWGG 8 cut(s) 182, 303, 1264, 1340, 1643, 3342, 3651, 3894
MvnI CGCG 2 cut(s) 84, 2175
MwoI GCNNNNNNNGC 9 cut(s) 554, 1066, 1133, 1145, 1856, 1999, 2498, 3464, 3717
NaeI GCCGGC 1 cut(s) 298
NciI CCSGG 3 cut(s) 1326, 2251, 3926
NgoMIV GCCGGC 1 cut(s) 296
NlaIV GGNNCC 7 cut(s) 21, 628, 963, 3226, 3534, 3648, 3900
NmeAIII GCCGAG 2 cut(s) 1839, 2043
NmuCI GTSAC 3 cut(s) 245, 305, 1204
NsiI ATGCAT 2 cut(s) 2408, 3361
NspI RCATGY 6 cut(s) 2158, 2464, 3363, 3474, 3574, 3784
NspV TTCGAA 1 cut(s) 924
PagI TCATGA 2 cut(s) 2878, 3753
PciI ACATGT 3 cut(s) 2154, 2460, 3570
PciSI GCTCTTC 1 cut(s) 921
PcsI WCGNNNNNNNCGW 2 cut(s) 84, 2302
PctI GAATGC 4 cut(s) 439, 1470, 1642, 3547
PdiI GCCGGC 1 cut(s) 298
PdmI GAANNNNTTC 2 cut(s) 1673, 3712
PflFI GACNNNGTC 2 cut(s) 83, 124
PflMI CCANNNNNTGG 1 cut(s) 1926
Ple19I CGATCG 1 cut(s) 30
PleI GAGTC 8 cut(s) 242, 461, 829, 866, 1201, 1694, 1733, 3196
PpsI GAGTC 8 cut(s) 242, 461, 829, 866, 1201, 1694, 1733, 3196
Ppu21I YACGTR 1 cut(s) 2467
PpuMI RGGWCCY 2 cut(s) 1186, 3344
PscI ACATGT 3 cut(s) 2154, 2460, 3570
PsiI TTATAA 1 cut(s) 1659
Psp124BI GAGCTC 2 cut(s) 796, 1614
Psp5II RGGWCCY 2 cut(s) 1186, 3344
Psp6I CCWGG 8 cut(s) 180, 301, 1262, 1338, 1641, 3340, 3649, 3892
PspFI CCCAGC 2 cut(s) 494, 3496
PspGI CCWGG 8 cut(s) 180, 301, 1262, 1338, 1641, 3340, 3649, 3892
PspN4I GGNNCC 7 cut(s) 21, 628, 963, 3226, 3534, 3648, 3900
PspPI GGNCC 7 cut(s) 20, 299, 1186, 1929, 2426, 2841, 3344
PspPPI RGGWCCY 2 cut(s) 1186, 3344
PstI CTGCAG 3 cut(s) 361, 3514, 3583
PstNI CAGNNNCTG 1 cut(s) 3347
PsuI RGATCY 3 cut(s) 940, 1034, 2922
PsyI GACNNNGTC 2 cut(s) 83, 124
PvuI CGATCG 1 cut(s) 30
PvuII CAGCTG 2 cut(s) 1139, 3584
RsaI GTAC 9 cut(s) 450, 1011, 1431, 2027, 2043, 2255, 2465, 3302, 3480
RsaNI GTAC 9 cut(s) 449, 1010, 1430, 2026, 2042, 2254, 2464, 3301, 3479
RseI CAYNNNNRTG 4 cut(s) 702, 2287, 2465, 2487
SacI GAGCTC 2 cut(s) 796, 1614
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 9 cut(s) 227, 2682, 2715, 2892, 3396, 3417, 3642, 3915, 4020
Sau96I GGNCC 7 cut(s) 20, 299, 1186, 1929, 2426, 2841, 3344
ScaI AGTACT 1 cut(s) 3302
SchI GAGTC 8 cut(s) 242, 461, 829, 866, 1201, 1695, 1733, 3197
SduI GDGCHC 8 cut(s) 522, 796, 1257, 1614, 2311, 2392, 3354, 3901
SexAI ACCWGGT 2 cut(s) 180, 3340
SfcI CTRYAG 6 cut(s) 52, 357, 1500, 3429, 3510, 3579
SfuI TTCGAA 1 cut(s) 924
SinI GGWCC 5 cut(s) 20, 1186, 1929, 2841, 3344
SmiMI CAYNNNNRTG 4 cut(s) 702, 2287, 2465, 2487
SmlI CTYRAG 3 cut(s) 1613, 2798, 3641
SmoI CTYRAG 3 cut(s) 1613, 2798, 3641
SspMI CTAG 4 cut(s) 1041, 2343, 3089, 3839
SstI GAGCTC 2 cut(s) 796, 1614
StyI CCWWGG 3 cut(s) 1218, 1421, 2377
TaiI ACGT 4 cut(s) 135, 1676, 2469, 2682
TatI WGTACW 5 cut(s) 1009, 2025, 2041, 3300, 3478
TauI GCSGC 2 cut(s) 173, 3605
Tru1I TTAA 9 cut(s) 227, 2682, 2715, 2892, 3396, 3417, 3642, 3915, 4020
Tru9I TTAA 9 cut(s) 227, 2682, 2715, 2892, 3396, 3417, 3642, 3915, 4020
TscAI CASTG 8 cut(s) 148, 432, 589, 853, 1012, 2044, 2494, 2676
TseFI GTSAC 3 cut(s) 245, 305, 1204
Tsp45I GTSAC 3 cut(s) 245, 305, 1204
TspGWI ACGGA 1 cut(s) 512
TspRI CASTG 8 cut(s) 148, 432, 589, 853, 1012, 2044, 2494, 2676
Tth111I GACNNNGTC 2 cut(s) 83, 124
Van91I CCANNNNNTGG 1 cut(s) 1926
Vha464I CTTAAG 1 cut(s) 3641
VpaK11BI GGWCC 5 cut(s) 20, 1186, 1929, 2841, 3344
XapI RAATTY 6 cut(s) 1407, 3000, 3010, 3197, 3636, 3793
XbaI TCTAGA 1 cut(s) 3088
XceI RCATGY 6 cut(s) 2158, 2464, 3363, 3474, 3574, 3784
XmnI GAANNNNTTC 2 cut(s) 1673, 3712
XspI CTAG 4 cut(s) 1041, 2343, 3089, 3839
ZrmI AGTACT 1 cut(s) 3302
Zsp2I ATGCAT 2 cut(s) 2408, 3361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.