Rmu_sc0002356.1_g000012

Pentatricopeptide repeat-containing protein At1g77360

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002356.1
OG0009496
Physical Location & Seq
Reverse (-)
62351 .. 63277
927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
atgatgtcttgccccacactcatacttgatactgcccttgatcaaagtgggctcagaatttcacctgaaatagtagaggatgttctcgtgagattcaagaatgccggaatggttgcataccgcttctttgaatgggcagcgaagcagcgaaactacacgcatagtgttaaggcataccatgctatgattgattctttggcaaagataaggcagtaccagatcatgtgggagcttgtgaactcaatgaggacccaaaggatgcagaatatggagacatttggtatcattatgaggaagtatgccagggctcagaaggttgaggaagcgatttatacttttaatgttatggagaagtatgattgtgctccgaatttagcagcattcaatggcctgctgagtgctttctgcaaatccaagaatgtgaggaaggctcaggagatttttgataaaatgaaggatcgctttgagccagactcaaatacgtatagtatattaattgatggatggggaaaggatccgaatttgcccaaggcaagggaggtctttagagaaatggttgatgctggctgtaatcctgatatagtgacttatggtatcatggttgatgttctgtgcaaagcagggagggtggacgaagccattgagattgttcggggcatggatgctagtggttgtatgcctacatcttttatttacagtgttttggtgcatacatatggggtggaaaataggattgaagacgctgttgaagcattcttggagatggaaaggaatggaatcaaggctgatgtagccttctataatgccttgattggtgctttttgtaaagttaacaagttcaagaatgtgtggagggtcttaaatgatatgacttccaaaggtgttacaggcttacagccaattcaaggacctgcaatatcatcttga

Protein Analysis

308

Amino Acids

34.96

Weight (kDa)

7.53

Isoelectric Point (pI)

28.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 74 - 153 3.1e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 91 - 137 2.5e-08 PPR repeat family
PPR_1 PF12854 120 - 152 4.4e-07 PPR repeat
PPR_2 PF13041 123 - 168 3.4e-12 PPR repeat family
PPR PF01535 127 - 154 9.5e-06 PPR repeat
PPR_1 PF12854 155 - 186 5.1e-06 PPR repeat
PPR_2 PF13041 157 - 206 8.4e-13 PPR repeat family
PPR_long PF17177 175 - 292 9.7e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 178 - 284 3.3e-08 Fungal tetratrico peptide repeats
PPR_3 PF13812 181 - 240 9.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 189 - 220 8.1e-13 PPR repeat
PPR PF01535 195 - 225 1.5e-06 PPR repeat
PPR_2 PF13041 263 - 295 8.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 919
AciI CCGC 1 cut(s) 121
AclWI GGATC 3 cut(s) 465, 509, 522
AcsI RAATTY 3 cut(s) 57, 370, 520
AfaI GTAC 1 cut(s) 215
AfiI CCNNNNNNNGG 2 cut(s) 534, 905
AgsI TTSAA 7 cut(s) 97, 131, 385, 737, 749, 841, 905
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 1 cut(s) 232
AluI AGCT 1 cut(s) 232
Alw21I GWGCWC 1 cut(s) 367
Alw26I GTCTC 1 cut(s) 266
AlwI GGATC 3 cut(s) 465, 509, 522
AoxI GGCC 1 cut(s) 388
ApeKI GCWGC 3 cut(s) 137, 145, 377
ApoI RAATTY 3 cut(s) 57, 370, 520
AseI ATTAAT 1 cut(s) 494
AspS9I GGNCC 2 cut(s) 249, 908
AsuHPI GGTGA 1 cut(s) 54
AvaII GGWCC 2 cut(s) 249, 908
BamHI GGATCC 1 cut(s) 514
BanII GRGCYC 2 cut(s) 54, 310
BauI CACGAG 1 cut(s) 86
BbsI GAAGAC 1 cut(s) 744
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 3 cut(s) 149, 157, 389
BccI CCATC 3 cut(s) 494, 498, 757
BciT130I CCWGG 1 cut(s) 304
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 266
BfaI CTAG 1 cut(s) 666
BfuAI ACCTGC 1 cut(s) 919
BisI GCNGC 3 cut(s) 138, 146, 378
BlsI GCNGC 3 cut(s) 139, 147, 379
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 2 cut(s) 249, 908
BmgT120I GGNCC 2 cut(s) 249, 908
BmiI GGNNCC 2 cut(s) 251, 516
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 3 cut(s) 249, 550, 652
BpiI GAAGAC 1 cut(s) 744
BplI GAGNNNNNCTC 4 cut(s) 415, 447, 458, 490
Bpu10I CCTNAGC 1 cut(s) 432
BsaAI YACGTR 1 cut(s) 483
BsaJI CCNNGG 2 cut(s) 303, 528
Bsc4I CCNNNNNNNGG 2 cut(s) 534, 905
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 303, 528
BseGI GGATG 4 cut(s) 85, 264, 509, 667
BseLI CCNNNNNNNGG 2 cut(s) 534, 905
BseMII CTCAG 4 cut(s) 67, 323, 386, 446
BseXI GCAGC 3 cut(s) 149, 157, 389
BshFI GGCC 1 cut(s) 390
BsiHKAI GWGCWC 1 cut(s) 367
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 2 cut(s) 534, 905
BsmAI GTCTC 1 cut(s) 266
BsmI GAATGC 3 cut(s) 106, 380, 752
BsnI GGCC 1 cut(s) 390
Bsp1286I GDGCHC 3 cut(s) 54, 310, 367
Bsp143I GATC 4 cut(s) 40, 219, 457, 514
BspACI CCGC 1 cut(s) 121
BspANI GGCC 1 cut(s) 390
BspCNI CTCAG 4 cut(s) 66, 322, 387, 445
BspLI GGNNCC 2 cut(s) 251, 516
BspMI ACCTGC 1 cut(s) 919
BspPI GGATC 3 cut(s) 465, 509, 522
BssECI CCNNGG 2 cut(s) 303, 528
BssMI GATC 4 cut(s) 40, 219, 457, 514
BssSI CACGAG 1 cut(s) 86
BssT1I CCWWGG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 1 cut(s) 698
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBAI YACGTR 1 cut(s) 483
BstC8I GCNNGC 2 cut(s) 392, 565
BstDEI CTNAG 4 cut(s) 53, 309, 395, 432
BstF5I GGATG 4 cut(s) 85, 264, 509, 667
BstKTI GATC 4 cut(s) 43, 222, 460, 517
BstMAI GTCTC 1 cut(s) 266
BstMBI GATC 4 cut(s) 40, 219, 457, 514
BstMWI GCNNNNNNNGC 3 cut(s) 179, 749, 791
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstSNI TACGTA 1 cut(s) 483
BstV1I GCAGC 3 cut(s) 149, 157, 389
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 1 cut(s) 514
BstYI RGATCY 1 cut(s) 514
BsuRI GGCC 1 cut(s) 390
BtsCI GGATG 4 cut(s) 85, 264, 509, 667
BtsIMutI CAGTG 1 cut(s) 703
BveI ACCTGC 1 cut(s) 919
Cac8I GCNNGC 2 cut(s) 392, 565
Cfr13I GGNCC 2 cut(s) 249, 908
CseI GACGC 1 cut(s) 749
Csp6I GTAC 1 cut(s) 214
CviAII CATG 4 cut(s) 179, 223, 598, 658
CviQI GTAC 1 cut(s) 214
DdeI CTNAG 4 cut(s) 53, 309, 395, 432
DpnI GATC 4 cut(s) 42, 221, 459, 516
DpnII GATC 4 cut(s) 40, 219, 457, 514
Eco105I TACGTA 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 528
Eco24I GRGCYC 2 cut(s) 54, 310
Eco47I GGWCC 2 cut(s) 249, 908
EcoO109I RGGNCCY 2 cut(s) 249, 908
EcoRII CCWGG 1 cut(s) 302
EcoT14I CCWWGG 1 cut(s) 528
EcoT38I GRGCYC 2 cut(s) 54, 310
ErhI CCWWGG 1 cut(s) 528
FaeI CATG 4 cut(s) 182, 226, 601, 661
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FatI CATG 4 cut(s) 178, 222, 597, 657
FauNDI CATATG 1 cut(s) 715
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 138, 146, 378
FokI GGATG 4 cut(s) 92, 271, 516, 674
FriOI GRGCYC 2 cut(s) 54, 310
Fsp4HI GCNGC 3 cut(s) 138, 146, 378
FspBI CTAG 1 cut(s) 666
GluI GCNGC 3 cut(s) 138, 146, 378
HaeIII GGCC 1 cut(s) 390
HapII CCGG 1 cut(s) 105
HgaI GACGC 1 cut(s) 749
Hin1II CATG 4 cut(s) 182, 226, 601, 661
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 4 cut(s) 93, 191, 473, 777
HpaI GTTAAC 1 cut(s) 832
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 54
Hpy166II GTNNAC 3 cut(s) 238, 631, 832
Hpy188I TCNGA 4 cut(s) 56, 312, 369, 519
Hpy188III TCNNGA 6 cut(s) 88, 97, 434, 575, 841, 924
Hpy8I GTNNAC 3 cut(s) 238, 631, 832
HpyAV CCTTC 4 cut(s) 307, 421, 448, 805
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 6 cut(s) 116, 262, 408, 615, 709, 914
HpyF10VI GCNNNNNNNGC 3 cut(s) 179, 749, 791
HpyF3I CTNAG 4 cut(s) 53, 309, 395, 432
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 4 cut(s) 182, 226, 601, 661
Ksp22I TGATCA 1 cut(s) 40
KspAI GTTAAC 1 cut(s) 832
Kzo9I GATC 4 cut(s) 40, 219, 457, 514
LmnI GCTCC 2 cut(s) 229, 370
Lsp1109I GCAGC 3 cut(s) 149, 157, 389
LweI GCATC 3 cut(s) 249, 550, 652
MaeI CTAG 1 cut(s) 666
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 2 cut(s) 583, 883
MalI GATC 4 cut(s) 42, 221, 459, 516
MboI GATC 4 cut(s) 40, 219, 457, 514
MboII GAAGA 1 cut(s) 749
MflI RGATCY 1 cut(s) 514
MhlI GDGCHC 3 cut(s) 54, 310, 367
MluCI AATT 5 cut(s) 57, 370, 495, 520, 900
MlyI GAGTC 1 cut(s) 467
MnlI CCTC 8 cut(s) 70, 240, 285, 313, 417, 532, 618, 846
MseI TTAA 5 cut(s) 168, 339, 494, 831, 860
MslI CAYNNNNRTG 1 cut(s) 714
MspI CCGG 1 cut(s) 105
MspR9I CCNGG 1 cut(s) 304
Mva1269I GAATGC 3 cut(s) 106, 380, 752
MvaI CCWGG 1 cut(s) 304
MwoI GCNNNNNNNGC 3 cut(s) 179, 749, 791
NdeI CATATG 1 cut(s) 715
NdeII GATC 4 cut(s) 40, 219, 457, 514
NlaIII CATG 4 cut(s) 182, 226, 601, 661
NlaIV GGNNCC 2 cut(s) 251, 516
NmuCI GTSAC 1 cut(s) 583
PctI GAATGC 3 cut(s) 106, 380, 752
PfeI GAWTC 3 cut(s) 93, 191, 777
PkrI GCNGC 3 cut(s) 139, 147, 379
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 2 cut(s) 249, 908
PshBI ATTAAT 1 cut(s) 494
Psp5II RGGWCCY 2 cut(s) 249, 908
Psp6I CCWGG 1 cut(s) 302
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 2 cut(s) 251, 516
PspPI GGNCC 2 cut(s) 249, 908
PspPPI RGGWCCY 2 cut(s) 249, 908
PsrI GAACNNNNNNTAC 2 cut(s) 66, 98
PsuI RGATCY 1 cut(s) 514
RsaI GTAC 1 cut(s) 215
RsaNI GTAC 1 cut(s) 214
RseI CAYNNNNRTG 1 cut(s) 714
SaqAI TTAA 5 cut(s) 168, 339, 494, 831, 860
SatI GCNGC 3 cut(s) 138, 146, 378
Sau3AI GATC 4 cut(s) 40, 219, 457, 514
Sau96I GGNCC 2 cut(s) 249, 908
SchI GAGTC 1 cut(s) 467
ScrFI CCNGG 1 cut(s) 304
SduI GDGCHC 3 cut(s) 54, 310, 367
SetI ASST 7 cut(s) 67, 234, 318, 485, 543, 883, 913
SfaNI GCATC 3 cut(s) 249, 550, 652
SinI GGWCC 2 cut(s) 249, 908
SmiMI CAYNNNNRTG 1 cut(s) 714
SnaBI TACGTA 1 cut(s) 483
Sse9I AATT 5 cut(s) 57, 370, 495, 520, 900
SsiI CCGC 1 cut(s) 121
SspMI CTAG 1 cut(s) 666
StyD4I CCNGG 1 cut(s) 302
StyI CCWWGG 1 cut(s) 528
TaaI ACNGT 1 cut(s) 698
TaiI ACGT 1 cut(s) 485
TasI AATT 5 cut(s) 57, 370, 495, 520, 900
TfiI GAWTC 3 cut(s) 93, 191, 777
Tru1I TTAA 5 cut(s) 168, 339, 494, 831, 860
Tru9I TTAA 5 cut(s) 168, 339, 494, 831, 860
TscAI CASTG 1 cut(s) 703
TseFI GTSAC 1 cut(s) 583
TseI GCWGC 3 cut(s) 137, 145, 377
Tsp45I GTSAC 1 cut(s) 583
TspDTI ATGAA 1 cut(s) 467
TspRI CASTG 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 249, 908
VspI ATTAAT 1 cut(s) 494
XapI RAATTY 3 cut(s) 57, 370, 520
XspI CTAG 1 cut(s) 666
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.