Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002454.1

Orthogroup ID

OG0006835

Physical Location & Seq

Genomic Coordinates Reverse (-)

8964 .. 25518

Gene Structure 16555 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5739 bp

atggcaccgtcgtccaaaaaacctcagggagggaataacaagaatcccagagacgctccaaagttcaacaaatcctccaaaaaacctttcaaaccaaacaaggatcacaacaacgccgccaggtccgaagccatggctttgcagctcgaagacgacgtccctgacttccccagaggcggagggagctcattgaatcgaaaagaacgcgacgaaattcgggcggaagttgatgccgaattcgaggcagaggagagggagatgaagaagaaagggaagaagaaaagggtgccgaaggacagccttgtaaacgaggacgagatgggctcgcttttcggcgatggcatcactggaaaactacctagatatgcaaacaagatcactttgaagaatatatcccctgggatgagggtttggggagttgttgccgaagtgaatgaaaaggaccttgtaatcagtcttccagggggcttgcgtggactagttcgagcgtctgatgcttttgatcctattctggatgatgaaactgaggccttagcagatagtgtcctccctagcatattccggatagggcagctggtatcttgcattgtgttgcaattggatgaggataagaaagagaaagggaaacggaagatctggctttctttgcgcctctctttgttgcataaaggcttcactttggattctgttcaagaaggcatggtccttacagcctatattaaaagcatcgaagaccatggttacattcttcattttggtttatcttcattcacagggtttttgccaaaaaatagccaagctgctcattctgttcttcttacagacagcaaggttcaagtgagcagtggtcaactgcttcaagctgctgttagaagagttgataaaacccgcaaggttgttcatatgagttctgacccagagatggtgtcaaagtgtgtgaccaaagatctcaagggaatttcaattgatctacttgttccggggatgatggtcaatgctcgtgtgttatccacccttgaaaatggggttatgttatcatttcttacttattttactggaactgttgatatatttcatctgcaaaatatttaccctactacaaactggaaggaagattacaatcaaaacaagaagctcaatgctcgagtattatttgtcgatccttcaactagagctgttggtttgacactaaatccacatcttgttcgcaacaaggctcctccttcacatgtcaaaattggggatatatgtgacgatgcaaaggtagtgagggttgatagaggattgggccttctgcttgaaataccatctactcaagtttcaactccggcatatgttagtatatctgatgtggctgaagaagttcggaagcttgaaaagaagttcaaggaaggtactcgtgttcgtgtcagaattcttgggtttaggcacttggaagggcttgccacagggattttgaaggcaagtgcatttgaagggtcagttttcactcactctgatgtcaaacctgggatggttgtgagaggtaaaataatagctgttgacagctttggggctatcgtgcaattccctggtggtgtgaaggctctttgtcctcttactcatatgtctgaatttgaaattgcaaagcccagaaaaaaatttaaggttggcgctgaacttgtgttccgtgtgcttggttgcaagtccaagagaataactgttacacacaagaaaacactcgtaaagtcaaagctagggattcttagttcatatgctgatgctgctgatggattaataacacatgggtggataagaaagattgaagaacgatctgacccaagttcaatgtatcatgttggacaagttatcaaatgtagagttctcggctctaatgctacatcgagacgaatcaaacttagcttgataatacggcctccaagggtttctgaggatgatatggtgaagctgggcagcctggtttcaggagttgttgaccgggtaacccccaatgctgtcatggtttatgttaatgcaaaaggttactcaatgggcacaatttttacagaccacttggcagatcaccatgggctggctgctttgatgaagtcagtattgaagcctggttatgaatttgatcaattattggttttagacactgaaggcaacaatttgatcctatctgcaaaacattctttgattaattcagctcccatgcttccttcagaagtcagtcaagttcatccaaatacggttgtacatggctacatttgtaacttaattgacactgggtgctttgttcgctttcttgggcgtgtgactggtttctccccaaggcacaaggcaatggatgaccataaaggggatctttcagaagcctattacattggacagtctgttcggagtactatactcgatgtcaatagtgaaacaggtaggataacactatcattgaagcagtcatcttgctcttcaacagatgcgtcctttatccgggaatactttattacagaggagaagattgcaaagctgcaagttttggattcaaaagaatccaaatcagattggtcagaaggattttccattggccgtgttgttgaagggaaggtccaggagtcaaaggacattggagttgttgtcagctttgagaaatataatgatgttttcggtttcatcacgcactatcaattagctggtaccactgtagaaacaggctccattgttcgagcagttgtacttgatgttgccaaggcagagcaccttgttgacttatcgctcaaaccagaatttattactaacttaaaacaagaaagctctaagagccaaactcatatgaagaaacgaagaagagaagtctcggatgatccagagatgcatgagaccgtaaatgctgtggttgagattgtgaaggagaattacctggttctttctatcccaaagtataactatgctgtaggatatgcatcaatttctgactacaacactcaaaagtttcctcagaaacagtttctgaatggacaaagtgtcaatactactgtcatggcccttccaagccctacaaccgttgggagattgcttttggtacttaattctttgagtgagtccgctgatacttccagttcaaagcgggcaaagaagaaatccagctacaaagtgggatctgtggtccaggcagagataactgaaataaagcctcttgaactaagattgaagtttggaatcggctttcatggacgggtccgtataacagaggtaaatgatgatcttttggaagatccatttaataacttcagaattgggcaaacggtcactgcaaggattgttgcaaagactaattattcagataacaacaaaaagagtttccagtgggatctgtcattgaaacctagtttgttaacaggttcctgtgaaatagaggaaaatattttgaaaggagatctcgatttctcaattggaaaacatgtcactggctatgtgtacaaggtggatgctgaatggatctggttaacaatatctcgaaatgttagggcccagcttttcattcttgacagcgcttgtgagcctagtgaacttcaagagtttcagaaacgctttcatgttggaaatgctgtatctggtcatgttttaagtgttagcaaggagaaaaagctattacggctggtttcatatccttttactcccatcactgataaagctgttgataatgaggtgtcaaagacagatgcaaatgttagcatgttgaatgcaactgctcatattcgcgagggatgtgttgtaggtgggagaattattaaaaaacttccaggtgttggtggattgactgtgcaaattggtccccatatgtatggcagggttcattatagtgagctttctgactcatggttgtccaacccattgtctggatatgaggaaggtcaatttgtcaaatgcaaggtcgttgaatgtagtcgctctggacagggaacttttcacattgacttgtcattacggtcttctttagttggcacactttgtcaagattccaatgaacctgacgatgacaagcatactcatactgaacggccggaaaagattgacgatctgaaacctaatatggtcatacagggttatgtgaaaaatgtttcatcaaaaggatgttttattttgctttcgcggaagcttgatgccaaaattctcgtctccaatttatccgatggatatgttgatgatcctgaaaaggaattcccagttggaaaacttgttaccggcagggtgtcatctgtggagcctttatcaaagagagttgaagttaccttgaagtcactgagtgcaagcagtttgccacaatctgcaaggaataatttggataccttgcatgtcggagatattatctctggcagggtaaaacgagttgagtcatatggattattcatcacaattgacaatacaaatgtggttggattatgccacgtgtcagagcttgcagaagataagaaggacaattttgaaactaaatacaaaacaggagagagggtgtcagcaaaagtattgaaggtggacaaggagaggcaccgagtttctctcggaatgaaggatttgtatattatggatagcgaccaaacacgtccaaaacaagatcttgatgaacctatcagacagactgatttggtggatgattctaagtcatttatgtgtcctgttggtagtttccttggggacctgaacatggatattgaacatgaaaatgcagagttccagtttctttcacaagcagaatctagagctttagttcctccacttgaagtcactcttgatgatgtagaccaaggcgatgatattgttagccaggatcaagagcttcctgatgcggaggacgctgtagatgacaagaaaaagaagctcacgaagaaaaaagccagggatgagagggagcatgagataagggctgctgaggaaagattattggagaaagatatacctaggactgatgaggaatttgagaaactagtgagaagttctccaaataacagttatgtttggataaaatatatggaatttgtgctctctatggctgatgttgagaaggcacgctctattgctaaaagggctttggagacgataaactttcgagaagagaacgagaagctcaatgtttgggtggcttattttaatttggaaagcaaatatggaagtcctccagaggaggctgtaatgaaagtcttccaaaaagcgttgcagtataatgatcccaaaaaggttcatctagcactcttgggagtctttgagaggtctgagcaacacaagttggctgatgaacttgctgataacatgattaagaggtttaagaagtcttgcaaggtttggctaaggcgggtacaaaggctcttggtgcaacagcaggatggggttcaggattttgtctctcgtgctgaaaagatccttcctaagcataagtatgtcaaattcttgtcacagacagccatcctggagttcaaatgtgggaatccagagagagggcgatccacgtttgaaaatattttgaggcaaaacccgaagagaacagacttgtggagtgtttatcttgatcaagaaattcgacttggagatactgatctgattcgtgcattgtttgagagggcaactagtttgagcctaccagctaaaaagatgaaattcttgttcaaaaaatatctggaatatgaggagcgtcacggtaacgaagagagggccaattacgtgaaacagaaggcaatgagttatgttgagaatacagtagcttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000460 GO:0000462 GO:0000466 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005886 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0007275 GO:0008134 GO:0008150 GO:0008152 GO:0009553 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0022613 GO:0030490 GO:0030684 GO:0031974 GO:0031981 GO:0032040 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042274 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0048229 GO:0048856 GO:0070013 GO:0071704 GO:0071840 GO:0071944 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

S1 Suf

Protein Analysis

1912

Amino Acids

214.04

Weight (kDa)

8.7

Isoelectric Point (pI)

36.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
S1_RRP5	PF23459	132 - 205	1.9e-06	RRP5 S1 domain
S1_RRP5	PF23459	326 - 397	1e-06	RRP5 S1 domain
OB_RRP5_4th	PF24685	406 - 496	4.2e-40	RRP5 OB-fold domain
S1	PF00575	501 - 565	3.7e-08	S1 RNA binding domain
S1	PF00575	742 - 812	1.8e-07	S1 RNA binding domain
OB_RRP5	PF24682	937 - 1024	1.4e-37	RRP5 OB-fold domain
S1_RRP5	PF23459	1354 - 1424	5e-07	RRP5 S1 domain
S1	PF00575	1445 - 1517	2.5e-16	S1 RNA binding domain
HAT_Syf1_CNRKL1_C	PF23231	1660 - 1909	2.2e-11	Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
Suf	PF05843	1810 - 1908	1e-06	Suppressor of forked protein (Suf)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0006835)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11964 AT3G11964
fragaria_vesca	FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470 FvH4_6g21470
malus_domestica	MD12G1051100.v1.1
prunus_persica	Prupe.7G085700_v2.0.a1 Prupe.7G085700_v2.0.a1
pyrus_communis	pycom12g04550
rosa_chinensis	RchiOBHm_Chr3g0478661
rosa_laevigata	RLG00000023635
rosa_multiflora	Rmu_sc0002454.1_g000002 Rmu_sc0023704.1_g000001
rosa_roxburghii	Rroxscaffold_6G00403220
rosa_rugosa	Rorug03G0166800 Rorug03G0166800 Rorug03G0166900
rosa_samantha	Rh3AG219000 Rh3BG250700 Rh3CG247500 Rh3DG243800
rosa_wichuraiana	Rw3G019690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	159
Acc65I	GGTACC	1 cut(s)	2675
AccB1I	GGYRCC	4 cut(s)	4, 286, 2675, 4527
AccB7I	CCANNNNNTGG	3 cut(s)	2071, 3788, 3878
AccI	GTMKAC	1 cut(s)	4779
AccII	CGCG	3 cut(s)	207, 3741, 4132
AccIII	TCCGGA	1 cut(s)	561
AciI	CCGC	9 cut(s)	117, 177, 221, 889, 3084, 3106, 4132, 4826, 5329
AcoI	YGGCCR	2 cut(s)	2566, 4040
AcuI	CTGAAG	4 cut(s)	1377, 2160, 2187, 3253
AcvI	CACGTG	1 cut(s)	4429
AcyI	GRCGYC	1 cut(s)	156
AdeI	CACNNNGTG	2 cut(s)	2271, 4286
AfaI	GTAC	8 cut(s)	1396, 2238, 2386, 2677, 2715, 3063, 3458, 5334
AfeI	AGCGCT	1 cut(s)	3532
AflIII	ACRYGT	4 cut(s)	1228, 3439, 4428, 4580
AhdI	GACNNNNNGTC	1 cut(s)	3002
AhlI	ACTAGT	3 cut(s)	478, 4963, 5600
AjiI	CACGTC	1 cut(s)	4583
AjuI	GAANNNNNNNTTGG	6 cut(s)	71, 103, 4191, 4223, 5541, 5573
AleI	CACNNNNGTG	1 cut(s)	1786
AloI	GAACNNNNNNTCC	6 cut(s)	1650, 1682, 1732, 1764, 2361, 2393
Alw21I	GWGCWC	3 cut(s)	188, 2739, 5022
Alw26I	GTCTC	7 cut(s)	45, 1879, 2839, 2852, 4162, 5066, 5383
AlwNI	CAGNNNCTG	1 cut(s)	2989
Ama87I	CYCGRG	1 cut(s)	1143
Aor13HI	TCCGGA	1 cut(s)	561
Aor51HI	AGCGCT	1 cut(s)	3532
AoxI	GGCC	8 cut(s)	528, 1288, 1913, 2566, 3021, 3507, 4040, 5685
ApaI	GGGCCC	1 cut(s)	3511
ApeKI	GCWGC	9 cut(s)	142, 571, 800, 863, 1763, 1953, 2075, 2509, 4904
ArsI	GACNNNNNNTTYG	8 cut(s)	141, 173, 2361, 2393, 3897, 3929, 4140, 4172
AseI	ATTAAT	2 cut(s)	1775, 2181
Asp700I	GAANNNNTTC	2 cut(s)	1362, 5177
Asp718I	GGTACC	1 cut(s)	2675
AspA2I	CCTAGG	1 cut(s)	4937
AspLEI	GCGC	3 cut(s)	651, 1655, 3533
AsuC2I	CCSGG	3 cut(s)	981, 1979, 2474
AsuHPI	GGTGA	2 cut(s)	1954, 2054
AvaI	CYCGRG	1 cut(s)	1143
AvaII	GGWCC	8 cut(s)	123, 442, 703, 2587, 3145, 3217, 3812, 4675
AvrII	CCTAGG	1 cut(s)	4937
AxyI	CCTNAGG	1 cut(s)	24
BaeGI	GKGCMC	2 cut(s)	2036, 3511
BaeI	ACNNNNGTAYC	4 cut(s)	1386, 1419, 2667, 2700
BanI	GGYRCC	4 cut(s)	4, 286, 2675, 4527
BanII	GRGCYC	3 cut(s)	188, 326, 3511
BarI	GAAGNNNNNNTAC	6 cut(s)	441, 473, 1100, 1132, 2879, 2911
BauI	CACGAG	3 cut(s)	999, 1398, 5382
BbrPI	CACGTG	1 cut(s)	4429
BbsI	GAAGAC	5 cut(s)	156, 449, 738, 3963, 5170
Bbv12I	GWGCWC	3 cut(s)	188, 2739, 5022
BbvCI	CCTCAGC	1 cut(s)	4908
BbvI	GCAGC	9 cut(s)	154, 583, 787, 850, 1750, 1965, 2062, 2496, 4891
BceAI	ACGGC	4 cut(s)	1928, 2553, 3650, 4055
BciVI	GTATCC	1 cut(s)	4318
BclI	TGATCA	2 cut(s)	2116, 5542
BcnI	CCSGG	3 cut(s)	981, 1979, 2474
BcoDI	GTCTC	7 cut(s)	45, 1879, 2839, 2852, 4162, 5066, 5383
BcuI	ACTAGT	3 cut(s)	478, 4963, 5600
BfmI	CTRYAG	3 cut(s)	2682, 2931, 4836
BfoI	RGCGCY	2 cut(s)	1656, 3534
BfuI	GTATCC	1 cut(s)	4318
BglII	AGATCT	4 cut(s)	633, 946, 3415, 4594
BlnI	CCTAGG	1 cut(s)	4937
BmcAI	AGTACT	1 cut(s)	2386
Bme18I	GGWCC	8 cut(s)	123, 442, 703, 2587, 3145, 3217, 3812, 4675
BmeRI	GACNNNNNGTC	1 cut(s)	3002
BmeT110I	CYCGRG	1 cut(s)	1143
BmgBI	CACGTC	1 cut(s)	4583
BmrI	ACTGGG	2 cut(s)	2277, 4199
BmuI	ACTGGG	2 cut(s)	2277, 4199
BpiI	GAAGAC	5 cut(s)	156, 449, 738, 3963, 5170
BplI	GAGNNNNNCTC	8 cut(s)	308, 340, 2791, 2823, 4524, 4556, 4960, 4992
BpmI	CTGGAG	2 cut(s)	5139, 5465
Bpu10I	CCTNAGC	4 cut(s)	532, 4908, 5324, 5403
BpuEI	CTTGAG	2 cut(s)	935, 1299
BpuMI	CCSGG	3 cut(s)	981, 1979, 2474
BsaAI	YACGTR	2 cut(s)	4429, 5695
BsaBI	GATNNNNATC	3 cut(s)	1119, 2941, 5568
BsaHI	GRCGYC	1 cut(s)	156
BsaI	GGTCTC	1 cut(s)	2852
BsaWI	WCCGGW	1 cut(s)	561
BsaXI	ACNNNNNCTCC	6 cut(s)	59, 89, 2601, 2631, 4476, 4506
Bse118I	RCCGGY	1 cut(s)	4223
Bse21I	CCTNAGG	1 cut(s)	24
Bse3DI	GCAATG	2 cut(s)	2331, 5715
Bse8I	GATNNNNATC	3 cut(s)	1119, 2941, 5568
BseAI	TCCGGA	1 cut(s)	561
BseJI	GATNNNNATC	3 cut(s)	1119, 2941, 5568
BseMI	GCAATG	2 cut(s)	2331, 5715
BseMII	CTCAG	7 cut(s)	38, 516, 1920, 2990, 4274, 4899, 5241
BseRI	GAGGAG	5 cut(s)	263, 1209, 2507, 5174, 5675
BseSI	GKGCMC	2 cut(s)	2036, 3511
BseX3I	CGGCCG	1 cut(s)	4040
BseXI	GCAGC	9 cut(s)	154, 583, 787, 850, 1750, 1965, 2062, 2496, 4891
BseYI	CCCAGC	2 cut(s)	1948, 3510
Bsh1236I	CGCG	3 cut(s)	207, 3741, 4132
Bsh1285I	CGRYCG	1 cut(s)	4043
BshFI	GGCC	8 cut(s)	530, 1290, 1915, 2568, 3023, 3509, 4042, 5687
BshNI	GGYRCC	4 cut(s)	4, 286, 2675, 4527
BsiEI	CGRYCG	1 cut(s)	4043
BsiHKAI	GWGCWC	3 cut(s)	188, 2739, 5022
BsiHKCI	CYCGRG	1 cut(s)	1143
BsiSI	CCGG	7 cut(s)	562, 980, 1328, 1978, 2473, 4043, 4224
BslFI	GGGAC	3 cut(s)	143, 3798, 4688
BsmAI	GTCTC	7 cut(s)	45, 1879, 2839, 2852, 4162, 5066, 5383
BsmBI	CGTCTC	4 cut(s)	45, 1879, 4162, 5066
BsmFI	GGGAC	3 cut(s)	143, 3798, 4688
BsmI	GAATGC	1 cut(s)	3727
BsnI	GGCC	8 cut(s)	530, 1290, 1915, 2568, 3023, 3509, 4042, 5687
Bso31I	GGTCTC	1 cut(s)	2852
BsoBI	CYCGRG	1 cut(s)	1143
Bsp120I	GGGCCC	1 cut(s)	3507
Bsp1286I	GDGCHC	6 cut(s)	188, 326, 2036, 2739, 3511, 5022
Bsp13I	TCCGGA	1 cut(s)	561
Bsp1407I	TGTACA	2 cut(s)	2236, 3456
Bsp19I	CCATGG	3 cut(s)	132, 736, 2065
Bsp68I	TCGCGA	1 cut(s)	3741
BspACI	CCGC	9 cut(s)	117, 177, 221, 889, 3084, 3106, 4132, 4826, 5329
BspANI	GGCC	8 cut(s)	530, 1290, 1915, 2568, 3023, 3509, 4042, 5687
BspCNI	CTCAG	7 cut(s)	37, 517, 1921, 2989, 4275, 4900, 5242
BspEI	TCCGGA	1 cut(s)	561
BspFNI	CGCG	3 cut(s)	207, 3741, 4132
BspQI	GCTCTTC	1 cut(s)	2455
BspT107I	GGYRCC	4 cut(s)	4, 286, 2675, 4527
BspTNI	GGTCTC	1 cut(s)	2852
BsrDI	GCAATG	2 cut(s)	2331, 5715
BsrFI	RCCGGY	1 cut(s)	4223
BsrGI	TGTACA	2 cut(s)	2236, 3456
BssAI	RCCGGY	1 cut(s)	4223
BssNI	GRCGYC	1 cut(s)	156
BssSI	CACGAG	3 cut(s)	999, 1398, 5382
BssT1I	CCWWGG	9 cut(s)	132, 736, 1919, 2065, 2312, 2727, 4669, 4783, 4937
Bst2BI	CACGAG	3 cut(s)	999, 1398, 5382
Bst6I	CTCTTC	6 cut(s)	868, 2455, 2821, 5085, 5507, 5673
BstACI	GRCGYC	1 cut(s)	156
BstAUI	TGTACA	2 cut(s)	2236, 3456
BstBAI	YACGTR	2 cut(s)	4429, 5695
BstC8I	GCNNGC	8 cut(s)	326, 470, 1443, 2073, 3108, 4291, 4440, 5047
BstDSI	CCRYGG	3 cut(s)	132, 736, 2065
BstEII	GGTNACC	1 cut(s)	1981
BstENI	CCTNNNNNAGG	1 cut(s)	27
BstFNI	CGCG	3 cut(s)	207, 3741, 4132
BstH2I	RGCGCY	2 cut(s)	1656, 3534
BstHHI	GCGC	3 cut(s)	651, 1655, 3533
BstMAI	GTCTC	7 cut(s)	45, 1879, 2839, 2852, 4162, 5066, 5383
BstMCI	CGRYCG	1 cut(s)	4043
BstNSI	RCATGY	4 cut(s)	1232, 3443, 3718, 4337
BstPI	GGTNACC	1 cut(s)	1981
BstSFI	CTRYAG	3 cut(s)	2682, 2931, 4836
BstSLI	GKGCMC	2 cut(s)	2036, 3511
BstUI	CGCG	3 cut(s)	207, 3741, 4132
BstV1I	GCAGC	9 cut(s)	154, 583, 787, 850, 1750, 1965, 2062, 2496, 4891
BstV2I	GAAGAC	5 cut(s)	156, 449, 738, 3963, 5170
BstXI	CCANNNNNNTGG	1 cut(s)	3824
BstZI	CGGCCG	1 cut(s)	4040
Bsu36I	CCTNAGG	1 cut(s)	24
BsuI	GTATCC	1 cut(s)	4318
BsuRI	GGCC	8 cut(s)	530, 1290, 1915, 2568, 3023, 3509, 4042, 5687
BtgI	CCRYGG	3 cut(s)	132, 736, 2065
BtgZI	GCGATG	2 cut(s)	351, 4803
BtrI	CACGTC	1 cut(s)	4583
BtsI	GCAGTG	2 cut(s)	850, 3288
BtuMI	TCGCGA	1 cut(s)	3741
Cac8I	GCNNGC	8 cut(s)	326, 470, 1443, 2073, 3108, 4291, 4440, 5047
CaiI	CAGNNNCTG	1 cut(s)	2989
CfoI	GCGC	3 cut(s)	651, 1655, 3533
Cfr10I	RCCGGY	1 cut(s)	4223
CseI	GACGC	5 cut(s)	62, 477, 2451, 4841, 5654
CsiI	ACCWGGT	1 cut(s)	2898
Csp6I	GTAC	8 cut(s)	1395, 2237, 2385, 2676, 2714, 3062, 3457, 5333
CviQI	GTAC	8 cut(s)	1395, 2237, 2385, 2676, 2714, 3062, 3457, 5333
DraIII	CACNNNGTG	2 cut(s)	2271, 4286
DriI	GACNNNNNGTC	1 cut(s)	3002
EaeI	YGGCCR	2 cut(s)	2566, 4040
EagI	CGGCCG	1 cut(s)	4040
Eam1104I	CTCTTC	6 cut(s)	868, 2455, 2821, 5085, 5507, 5673
Eam1105I	GACNNNNNGTC	1 cut(s)	3002
EarI	CTCTTC	6 cut(s)	868, 2455, 2821, 5085, 5507, 5673
EciI	GGCGGA	2 cut(s)	192, 236
Ecl136II	GAGCTC	1 cut(s)	186
EclXI	CGGCCG	1 cut(s)	4040
Eco130I	CCWWGG	9 cut(s)	132, 736, 1919, 2065, 2312, 2727, 4669, 4783, 4937
Eco147I	AGGCCT	1 cut(s)	530
Eco24I	GRGCYC	3 cut(s)	188, 326, 3511
Eco31I	GGTCTC	1 cut(s)	2852
Eco47I	GGWCC	8 cut(s)	123, 442, 703, 2587, 3145, 3217, 3812, 4675
Eco47III	AGCGCT	1 cut(s)	3532
Eco52I	CGGCCG	1 cut(s)	4040
Eco53kI	GAGCTC	1 cut(s)	186
Eco57I	CTGAAG	4 cut(s)	1377, 2160, 2187, 3253
Eco72I	CACGTG	1 cut(s)	4429
Eco81I	CCTNAGG	1 cut(s)	24
Eco88I	CYCGRG	1 cut(s)	1143
Eco91I	GGTNACC	1 cut(s)	1981
EcoICRI	GAGCTC	1 cut(s)	186
EcoNI	CCTNNNNNAGG	1 cut(s)	27
EcoO109I	RGGNCCY	3 cut(s)	442, 3507, 4675
EcoO65I	GGTNACC	1 cut(s)	1981
EcoRI	GAATTC	3 cut(s)	236, 1413, 4199
EcoT14I	CCWWGG	9 cut(s)	132, 736, 1919, 2065, 2312, 2727, 4669, 4783, 4937
EcoT22I	ATGCAT	2 cut(s)	2856, 2944
EcoT38I	GRGCYC	3 cut(s)	188, 326, 3511
ErhI	CCWWGG	9 cut(s)	132, 736, 1919, 2065, 2312, 2727, 4669, 4783, 4937
Esp3I	CGTCTC	4 cut(s)	45, 1879, 4162, 5066
FalI	AAGNNNNNCTT	4 cut(s)	2331, 2363, 4752, 4784
FaqI	GGGAC	3 cut(s)	143, 3798, 4688
FauI	CCCGC	3 cut(s)	896, 3099, 5322
FauNDI	CATATG	7 cut(s)	903, 1333, 1605, 1753, 2811, 3819, 4378
FbaI	TGATCA	2 cut(s)	2116, 5542
FblI	GTMKAC	1 cut(s)	4779
FriOI	GRGCYC	3 cut(s)	188, 326, 3511
GlaI	GCGC	3 cut(s)	650, 1654, 3532
GsaI	CCCAGC	2 cut(s)	1952, 3514
GsuI	CTGGAG	2 cut(s)	5139, 5465
HaeII	RGCGCY	2 cut(s)	1656, 3534
HaeIII	GGCC	8 cut(s)	530, 1290, 1915, 2568, 3023, 3509, 4042, 5687
HapII	CCGG	7 cut(s)	562, 980, 1328, 1978, 2473, 4043, 4224
HgaI	GACGC	5 cut(s)	62, 477, 2451, 4841, 5654
HhaI	GCGC	3 cut(s)	651, 1655, 3533
Hin1I	GRCGYC	1 cut(s)	156
Hin6I	GCGC	3 cut(s)	649, 1653, 3531
HinP1I	GCGC	3 cut(s)	649, 1653, 3531
HincII	GTYRAC	6 cut(s)	851, 1543, 1975, 2746, 3375, 3486
HindII	GTYRAC	6 cut(s)	851, 1543, 1975, 2746, 3375, 3486
HindIII	AAGCTT	2 cut(s)	1370, 4136
HpaI	GTTAAC	2 cut(s)	3375, 3486
HpaII	CCGG	7 cut(s)	562, 980, 1328, 1978, 2473, 4043, 4224
HphI	GGTGA	2 cut(s)	1954, 2054
Hpy99I	CGWCG	3 cut(s)	13, 158, 212
HpyCH4IV	ACGT	5 cut(s)	156, 4428, 4582, 5483, 5694
HpySE526I	ACGT	5 cut(s)	156, 4428, 4582, 5483, 5694
Hsp92I	GRCGYC	1 cut(s)	156
HspAI	GCGC	3 cut(s)	649, 1653, 3531
Kpn2I	TCCGGA	1 cut(s)	561
KpnI	GGTACC	1 cut(s)	2679
Ksp22I	TGATCA	2 cut(s)	2116, 5542
KspAI	GTTAAC	2 cut(s)	3375, 3486
LguI	GCTCTTC	1 cut(s)	2455
LmnI	GCTCC	8 cut(s)	61, 183, 1222, 2194, 2699, 4243, 4888, 5662
Lsp1109I	GCAGC	9 cut(s)	154, 583, 787, 850, 1750, 1965, 2062, 2496, 4891
MabI	ACCWGGT	1 cut(s)	2898
MaeII	ACGT	5 cut(s)	156, 4428, 4582, 5483, 5694
MfeI	CAATTG	4 cut(s)	596, 963, 3429, 4395
MhlI	GDGCHC	6 cut(s)	188, 326, 2036, 2739, 3511, 5022
MlyI	GAGTC	5 cut(s)	2603, 3089, 3848, 4382, 5244
MmeI	TCCRAC	6 cut(s)	1819, 3559, 3891, 4189, 4318, 4396
Mph1103I	ATGCAT	2 cut(s)	2856, 2944
MroI	TCCGGA	1 cut(s)	561
MroXI	GAANNNNTTC	2 cut(s)	1362, 5177
MslI	CAYNNNNRTG	7 cut(s)	1497, 1786, 3822, 3840, 4648, 4701, 5412
MspA1I	CMGCKG	2 cut(s)	574, 3086
MspI	CCGG	7 cut(s)	562, 980, 1328, 1978, 2473, 4043, 4224
MunI	CAATTG	4 cut(s)	596, 963, 3429, 4395
Mva1269I	GAATGC	1 cut(s)	3727
MvnI	CGCG	3 cut(s)	207, 3741, 4132
NciI	CCSGG	3 cut(s)	981, 1979, 2474
NcoI	CCATGG	3 cut(s)	132, 736, 2065
NdeI	CATATG	7 cut(s)	903, 1333, 1605, 1753, 2811, 3819, 4378
NmeAIII	GCCGAG	1 cut(s)	1845
NmuCI	GTSAC	9 cut(s)	937, 1250, 2296, 3286, 3442, 4278, 4762, 5427, 5666
NruI	TCGCGA	1 cut(s)	3741
NsiI	ATGCAT	2 cut(s)	2856, 2944
NspI	RCATGY	4 cut(s)	1232, 3443, 3718, 4337
OliI	CACNNNNGTG	1 cut(s)	1786
PaeR7I	CTCGAG	1 cut(s)	1143
PasI	CCCWGGG	1 cut(s)	398
PceI	AGGCCT	1 cut(s)	530
PciI	ACATGT	2 cut(s)	1228, 3439
PciSI	GCTCTTC	1 cut(s)	2455
PcsI	WCGNNNNNNNCGW	1 cut(s)	153
PctI	GAATGC	1 cut(s)	3727
PdmI	GAANNNNTTC	2 cut(s)	1362, 5177
PflFI	GACNNNGTC	1 cut(s)	155
PflMI	CCANNNNNTGG	3 cut(s)	2071, 3788, 3878
PfoI	TCCNGGA	3 cut(s)	2472, 2589, 5442
PleI	GAGTC	5 cut(s)	2602, 3088, 3848, 4381, 5243
PmaCI	CACGTG	1 cut(s)	4429
PmlI	CACGTG	1 cut(s)	4429
PpsI	GAGTC	5 cut(s)	2602, 3088, 3848, 4381, 5243
Ppu21I	YACGTR	2 cut(s)	4429, 5695
PpuMI	RGGWCCY	2 cut(s)	442, 4675
PscI	ACATGT	2 cut(s)	1228, 3439
PshBI	ATTAAT	2 cut(s)	1775, 2181
Psp124BI	GAGCTC	1 cut(s)	188
Psp5II	RGGWCCY	2 cut(s)	442, 4675
PspCI	CACGTG	1 cut(s)	4429
PspEI	GGTNACC	1 cut(s)	1981
PspFI	CCCAGC	2 cut(s)	1948, 3510
PspOMI	GGGCCC	1 cut(s)	3507
PspPPI	RGGWCCY	2 cut(s)	442, 4675
PspXI	VCTCGAGB	1 cut(s)	1143
PstNI	CAGNNNCTG	1 cut(s)	2989
PsyI	GACNNNGTC	1 cut(s)	155
PvuII	CAGCTG	1 cut(s)	574
RruI	TCGCGA	1 cut(s)	3741
RsaI	GTAC	8 cut(s)	1396, 2238, 2386, 2677, 2715, 3063, 3458, 5334
RsaNI	GTAC	8 cut(s)	1395, 2237, 2385, 2676, 2714, 3062, 3457, 5333
RseI	CAYNNNNRTG	7 cut(s)	1497, 1786, 3822, 3840, 4648, 4701, 5412
SacI	GAGCTC	1 cut(s)	188
SapI	GCTCTTC	1 cut(s)	2455
ScaI	AGTACT	1 cut(s)	2386
SchI	GAGTC	5 cut(s)	2603, 3089, 3848, 4382, 5244
SduI	GDGCHC	6 cut(s)	188, 326, 2036, 2739, 3511, 5022
SexAI	ACCWGGT	1 cut(s)	2898
SfcI	CTRYAG	3 cut(s)	2682, 2931, 4836
Sfr274I	CTCGAG	1 cut(s)	1143
SinI	GGWCC	8 cut(s)	123, 442, 703, 2587, 3145, 3217, 3812, 4675
SlaI	CTCGAG	1 cut(s)	1143
SmiMI	CAYNNNNRTG	7 cut(s)	1497, 1786, 3822, 3840, 4648, 4701, 5412
SmlI	CTYRAG	3 cut(s)	950, 1143, 1314
SmoI	CTYRAG	3 cut(s)	950, 1143, 1314
SpeI	ACTAGT	3 cut(s)	478, 4963, 5600
SseBI	AGGCCT	1 cut(s)	530
SsiI	CCGC	9 cut(s)	117, 177, 221, 889, 3084, 3106, 4132, 4826, 5329
SspI	AATATT	3 cut(s)	1087, 3403, 5494
SstI	GAGCTC	1 cut(s)	188
StuI	AGGCCT	1 cut(s)	530
StyI	CCWWGG	9 cut(s)	132, 736, 1919, 2065, 2312, 2727, 4669, 4783, 4937
TaiI	ACGT	5 cut(s)	159, 4431, 4585, 5486, 5697
TatI	WGTACW	4 cut(s)	2236, 2384, 2713, 3456
TauI	GCSGC	1 cut(s)	119
TseFI	GTSAC	9 cut(s)	937, 1250, 2296, 3286, 3442, 4278, 4762, 5427, 5666
TseI	GCWGC	9 cut(s)	142, 571, 800, 863, 1763, 1953, 2075, 2509, 4904
Tsp45I	GTSAC	9 cut(s)	937, 1250, 2296, 3286, 3442, 4278, 4762, 5427, 5666
TspGWI	ACGGA	3 cut(s)	643, 1658, 3209
Tth111I	GACNNNGTC	1 cut(s)	155
Van91I	CCANNNNNTGG	3 cut(s)	2071, 3788, 3878
VpaK11BI	GGWCC	8 cut(s)	123, 442, 703, 2587, 3145, 3217, 3812, 4675
VspI	ATTAAT	2 cut(s)	1775, 2181
XagI	CCTNNNNNAGG	1 cut(s)	27
XbaI	TCTAGA	1 cut(s)	4736
XceI	RCATGY	4 cut(s)	1232, 3443, 3718, 4337
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1996, 3130
XhoI	CTCGAG	1 cut(s)	1143
XmaJI	CCTAGG	1 cut(s)	4937
XmiI	GTMKAC	1 cut(s)	4779
XmnI	GAANNNNTTC	2 cut(s)	1362, 5177
ZraI	GACGTC	1 cut(s)	157
ZrmI	AGTACT	1 cut(s)	2386
Zsp2I	ATGCAT	2 cut(s)	2856, 2944

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.