Rmu_sc0002532.1_g000012
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. gTMT family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002532.1
OG0011326
Physical Location & Seq
Reverse (-)
56424 .. 58716
2293 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1044 bp
atggcatctacgaggctgaactaccagacgagcgggtggtcctcgccagcagcaaccacgcttcctcgtcgcacgtgtctgggtgtggctggcgtgaacggagcagcggtgagctcgaagaagaagaagaaggagagcgggtttgagttgagagcagtggcggcgacggcgatggaggcagtggagctgaagaaggacatagccgagttctacgacgagtcgtctgggttatgggaggacctctggggcgaccacatgcaccacggcttctacgaccccaaggccgccgacgtttcggtgtcggaccaccgcgcggcccagacccggatgatcgacgaggtgctccggttcgcagggatttctggcaagtgtccaattgggaatgtagtggatgttgggtgtgggattggagggagctccagatatctggctagcaaatacggggccaattgcaaagggattactctgagtcccgtacaggctctgagggccaatgctcttgctgctgctcaaggcctagccaataaggcatcctttcaagttgctgatgctctagcacaaccatttcctgatggccaatttgatctggtttggtccatggagagtggggaacacatgcctgataaagcaaagtttgttgatgagttggttcgagttgcggcaccaggagcaacgataatactagttacatggtgccacagggatcttggtgcgtctgaacaatcattgcagccagatgagaaaaagctcctggacaagatatgcaatgctttctatcttcctgcttggtgttctactgctgactacctcaagctactcgagtcttactcactcgaggatatcaaggctgaggactggtctccgtatgttgccccattttggccggcagtgattcgctcagcattgacatttaagggtttcttttctctgttgcgcacaggaatgaaaacaataagaggagcattggcaatgccattgatgatccaaggattcaagaaggatctaattaagtattccattattacatgccgaaaacctgaatag

Protein Analysis

347

Amino Acids

37.8

Weight (kDa)

8.03

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CMAS PF02353 65 - 228 6.2e-11 Mycolic acid cyclopropane synthetase
Methyltransf_23 PF13489 105 - 244 1.4e-12 Methyltransferase domain
Methyltransf_31 PF13847 128 - 239 2.5e-12 Methyltransferase domain
Methyltransf_25 PF13649 129 - 223 1.6e-17 Methyltransferase domain
Methyltransf_11 PF08241 130 - 227 1.8e-19 Methyltransferase domain
Methyltransf_12 PF08242 130 - 225 2.5e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 935
AccB1I GGYRCC 2 cut(s) 661, 693
AccBSI CCGCTC 2 cut(s) 33, 138
AccII CGCG 2 cut(s) 312, 314
AciI CCGC 8 cut(s) 33, 107, 138, 161, 285, 310, 314, 659
AclWI GGATC 3 cut(s) 711, 976, 1008
AcoI YGGCCR 2 cut(s) 574, 881
AcuI CTGAAG 1 cut(s) 209
AcvI CACGTG 1 cut(s) 75
AfaI GTAC 1 cut(s) 477
AfiI CCNNNNNNNGG 3 cut(s) 313, 324, 879
AflIII ACRYGT 1 cut(s) 74
AgsI TTSAA 2 cut(s) 539, 994
AhdI GACNNNNNGTC 1 cut(s) 220
AhlI ACTAGT 1 cut(s) 682
AjnI CCWGG 2 cut(s) 664, 750
AluBI AGCT 5 cut(s) 114, 187, 417, 748, 814
AluI AGCT 5 cut(s) 114, 187, 417, 748, 814
Alw21I GWGCWC 3 cut(s) 116, 345, 419
Alw26I GTCTC 1 cut(s) 864
AlwI GGATC 3 cut(s) 711, 976, 1008
AlwNI CAGNNNCTG 1 cut(s) 484
Ama87I CYCGRG 2 cut(s) 818, 833
AoxI GGCC 7 cut(s) 282, 315, 444, 489, 514, 574, 881
ApeKI GCWGC 5 cut(s) 50, 104, 503, 506, 730
AspLEI GCGC 2 cut(s) 314, 936
AspS9I GGNCC 7 cut(s) 39, 238, 304, 316, 444, 489, 594
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 1 cut(s) 121
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 2 cut(s) 818, 833
AvaII GGWCC 4 cut(s) 39, 238, 304, 594
BalI TGGCCA 1 cut(s) 576
BanI GGYRCC 2 cut(s) 661, 693
BanII GRGCYC 2 cut(s) 116, 419
BbrPI CACGTG 1 cut(s) 75
Bbv12I GWGCWC 3 cut(s) 116, 345, 419
BbvCI CCTCAGC 1 cut(s) 849
BbvI GCAGC 5 cut(s) 62, 116, 490, 493, 742
BccI CCATC 2 cut(s) 166, 566
BceAI ACGGC 2 cut(s) 183, 280
BciT130I CCWGG 2 cut(s) 666, 752
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 864
BcuI ACTAGT 1 cut(s) 682
BfaI CTAG 4 cut(s) 432, 518, 554, 683
BglI GCCNNNNNGGC 1 cut(s) 527
BisI GCNGC 9 cut(s) 51, 105, 162, 285, 315, 504, 507, 660, 731
BlpI GCTNAGC 1 cut(s) 898
BlsI GCNGC 9 cut(s) 52, 106, 163, 286, 316, 505, 508, 661, 732
Bme1390I CCNGG 3 cut(s) 325, 666, 752
Bme18I GGWCC 4 cut(s) 39, 238, 304, 594
BmeRI GACNNNNNGTC 1 cut(s) 220
BmeT110I CYCGRG 2 cut(s) 818, 833
BmgT120I GGNCC 7 cut(s) 39, 238, 304, 316, 444, 489, 594
BmiI GGNNCC 3 cut(s) 445, 663, 695
BmrFI CCNGG 3 cut(s) 325, 666, 752
BmsI GCATC 3 cut(s) 14, 538, 539
BmtI GCTAGC 1 cut(s) 435
BplI GAGNNNNNCTC 2 cut(s) 812, 844
BpmI CTGGAG 1 cut(s) 403
Bpu10I CCTNAGC 1 cut(s) 849
Bpu1102I GCTNAGC 1 cut(s) 898
BpuEI CTTGAG 2 cut(s) 495, 794
BpuMI CCSGG 1 cut(s) 325
BsaAI YACGTR 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 864
BsaJI CCNNGG 4 cut(s) 262, 279, 597, 985
BsaWI WCCGGW 1 cut(s) 345
BsaXI ACNNNNNCTCC 4 cut(s) 93, 123, 125, 155
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 324, 879
Bse118I RCCGGY 1 cut(s) 883
Bse1I ACTGG 1 cut(s) 860
Bse3DI GCAATG 3 cut(s) 725, 772, 975
BseBI CCWGG 2 cut(s) 666, 752
BseDI CCNNGG 4 cut(s) 262, 279, 597, 985
BseGI GGATG 3 cut(s) 333, 397, 530
BseLI CCNNNNNNNGG 3 cut(s) 313, 324, 879
BseMI GCAATG 3 cut(s) 725, 772, 975
BseMII CTCAG 4 cut(s) 458, 476, 840, 912
BseNI ACTGG 1 cut(s) 860
BseRI GAGGAG 1 cut(s) 972
BseXI GCAGC 5 cut(s) 62, 116, 490, 493, 742
Bsh1236I CGCG 2 cut(s) 312, 314
BshFI GGCC 7 cut(s) 284, 317, 446, 491, 516, 576, 883
BshNI GGYRCC 2 cut(s) 661, 693
BsiHKAI GWGCWC 3 cut(s) 116, 345, 419
BsiHKCI CYCGRG 2 cut(s) 818, 833
BsiSI CCGG 3 cut(s) 325, 346, 884
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 3 cut(s) 313, 324, 879
BsmAI GTCTC 1 cut(s) 864
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 7 cut(s) 284, 317, 446, 491, 516, 576, 883
Bso31I GGTCTC 1 cut(s) 864
BsoBI CYCGRG 2 cut(s) 818, 833
Bsp1286I GDGCHC 3 cut(s) 116, 345, 419
Bsp143I GATC 5 cut(s) 330, 583, 703, 981, 1000
Bsp1720I GCTNAGC 1 cut(s) 898
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 8 cut(s) 33, 107, 138, 161, 285, 310, 314, 659
BspANI GGCC 7 cut(s) 284, 317, 446, 491, 516, 576, 883
BspCNI CTCAG 4 cut(s) 459, 477, 841, 911
BspFNI CGCG 2 cut(s) 312, 314
BspLI GGNNCC 3 cut(s) 445, 663, 695
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 3 cut(s) 711, 976, 1008
BspT107I GGYRCC 2 cut(s) 661, 693
BspTNI GGTCTC 1 cut(s) 864
BsrBI CCGCTC 2 cut(s) 33, 138
BsrDI GCAATG 3 cut(s) 725, 772, 975
BsrFI RCCGGY 1 cut(s) 883
BsrI ACTGG 1 cut(s) 860
BssAI RCCGGY 1 cut(s) 883
BssECI CCNNGG 4 cut(s) 262, 279, 597, 985
BssMI GATC 5 cut(s) 330, 583, 703, 981, 1000
BssT1I CCWWGG 3 cut(s) 279, 597, 985
Bst2UI CCWGG 2 cut(s) 666, 752
BstBAI YACGTR 1 cut(s) 75
BstC8I GCNNGC 4 cut(s) 48, 91, 433, 885
BstDEI CTNAG 4 cut(s) 467, 485, 849, 898
BstDSI CCRYGG 2 cut(s) 262, 597
BstF5I GGATG 3 cut(s) 333, 397, 530
BstFNI CGCG 2 cut(s) 312, 314
BstHHI GCGC 2 cut(s) 314, 936
BstKTI GATC 5 cut(s) 333, 586, 706, 984, 1003
BstMAI GTCTC 1 cut(s) 864
BstMBI GATC 5 cut(s) 330, 583, 703, 981, 1000
BstMWI GCNNNNNNNGC 7 cut(s) 161, 167, 176, 488, 503, 527, 668
BstNI CCWGG 2 cut(s) 666, 752
BstNSI RCATGY 3 cut(s) 259, 619, 1029
BstSCI CCNGG 3 cut(s) 323, 664, 750
BstUI CGCG 2 cut(s) 312, 314
BstV1I GCAGC 5 cut(s) 62, 116, 490, 493, 742
BstX2I RGATCY 2 cut(s) 703, 1000
BstXI CCANNNNNNTGG 1 cut(s) 427
BstYI RGATCY 2 cut(s) 703, 1000
BsuRI GGCC 7 cut(s) 284, 317, 446, 491, 516, 576, 883
BtgI CCRYGG 2 cut(s) 262, 597
BtgZI GCGATG 1 cut(s) 185
BtsCI GGATG 3 cut(s) 333, 397, 530
BtsI GCAGTG 3 cut(s) 162, 186, 894
BtsIMutI CAGTG 3 cut(s) 162, 186, 894
Cac8I GCNNGC 4 cut(s) 48, 91, 433, 885
CaiI CAGNNNCTG 1 cut(s) 484
CfoI GCGC 2 cut(s) 314, 936
Cfr10I RCCGGY 1 cut(s) 883
Cfr13I GGNCC 7 cut(s) 39, 238, 304, 316, 444, 489, 594
CseI GACGC 1 cut(s) 702
Csp6I GTAC 1 cut(s) 476
CviAII CATG 5 cut(s) 256, 598, 616, 690, 1026
CviQI GTAC 1 cut(s) 476
DdeI CTNAG 4 cut(s) 467, 485, 849, 898
DpnI GATC 5 cut(s) 332, 585, 705, 983, 1002
DpnII GATC 5 cut(s) 330, 583, 703, 981, 1000
DriI GACNNNNNGTC 1 cut(s) 220
EaeI YGGCCR 2 cut(s) 574, 881
Eam1105I GACNNNNNGTC 1 cut(s) 220
Ecl136II GAGCTC 2 cut(s) 114, 417
Eco130I CCWWGG 3 cut(s) 279, 597, 985
Eco147I AGGCCT 1 cut(s) 516
Eco24I GRGCYC 2 cut(s) 116, 419
Eco31I GGTCTC 1 cut(s) 864
Eco32I GATATC 2 cut(s) 425, 841
Eco47I GGWCC 4 cut(s) 39, 238, 304, 594
Eco53kI GAGCTC 2 cut(s) 114, 417
Eco57I CTGAAG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 75
Eco88I CYCGRG 2 cut(s) 818, 833
EcoICRI GAGCTC 2 cut(s) 114, 417
EcoO109I RGGNCCY 1 cut(s) 238
EcoRII CCWGG 2 cut(s) 664, 750
EcoRV GATATC 2 cut(s) 425, 841
EcoT14I CCWWGG 3 cut(s) 279, 597, 985
EcoT38I GRGCYC 2 cut(s) 116, 419
ErhI CCWWGG 3 cut(s) 279, 597, 985
FaeI CATG 5 cut(s) 259, 601, 619, 693, 1029
FaiI YATR 9 cut(s) 200, 232, 257, 599, 617, 691, 763, 867, 1027
FalI AAGNNNNNCTT 4 cut(s) 518, 550, 905, 937
FaqI GGGAC 1 cut(s) 456
FatI CATG 5 cut(s) 255, 597, 615, 689, 1025
FauI CCCGC 2 cut(s) 26, 131
Fnu4HI GCNGC 9 cut(s) 51, 105, 162, 285, 315, 504, 507, 660, 731
FokI GGATG 3 cut(s) 340, 404, 517
FriOI GRGCYC 2 cut(s) 116, 419
Fsp4HI GCNGC 9 cut(s) 51, 105, 162, 285, 315, 504, 507, 660, 731
FspBI CTAG 4 cut(s) 432, 518, 554, 683
FspI TGCGCA 1 cut(s) 935
GlaI GCGC 2 cut(s) 313, 935
GluI GCNGC 9 cut(s) 51, 105, 162, 285, 315, 504, 507, 660, 731
GsuI CTGGAG 1 cut(s) 403
HaeIII GGCC 7 cut(s) 284, 317, 446, 491, 516, 576, 883
HapII CCGG 3 cut(s) 325, 346, 884
HgaI GACGC 1 cut(s) 702
HhaI GCGC 2 cut(s) 314, 936
Hin1II CATG 5 cut(s) 259, 601, 619, 693, 1029
Hin6I GCGC 2 cut(s) 312, 934
HinP1I GCGC 2 cut(s) 312, 934
HinfI GANTC 5 cut(s) 218, 469, 821, 892, 990
HpaII CCGG 3 cut(s) 325, 346, 884
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 4 cut(s) 304, 468, 486, 718
Hpy188III TCNNGA 3 cut(s) 420, 569, 994
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 5 cut(s) 72, 169, 218, 293, 338
HpyAV CCTTC 3 cut(s) 124, 187, 991
HpyCH4IV ACGT 2 cut(s) 74, 291
HpyCH4V TGCA 4 cut(s) 259, 453, 730, 765
HpyF10VI GCNNNNNNNGC 7 cut(s) 161, 167, 176, 488, 503, 527, 668
HpyF3I CTNAG 4 cut(s) 467, 485, 849, 898
HpySE526I ACGT 2 cut(s) 74, 291
Hsp92II CATG 5 cut(s) 259, 601, 619, 693, 1029
HspAI GCGC 2 cut(s) 312, 934
KroI GCCGGC 1 cut(s) 883
KroNI GCCGGC 1 cut(s) 885
Kzo9I GATC 5 cut(s) 330, 583, 703, 981, 1000
LmnI GCTCC 8 cut(s) 101, 184, 348, 414, 422, 668, 753, 959
Lsp1109I GCAGC 5 cut(s) 62, 116, 490, 493, 742
LweI GCATC 3 cut(s) 14, 538, 539
MaeI CTAG 4 cut(s) 432, 518, 554, 683
MaeII ACGT 2 cut(s) 74, 291
MaeIII GTNAC 1 cut(s) 685
MalI GATC 5 cut(s) 332, 585, 705, 983, 1002
MbiI CCGCTC 2 cut(s) 33, 138
MboI GATC 5 cut(s) 330, 583, 703, 981, 1000
MboII GAAGA 6 cut(s) 130, 133, 136, 139, 202, 770
MfeI CAATTG 2 cut(s) 375, 448
MflI RGATCY 2 cut(s) 703, 1000
MhlI GDGCHC 3 cut(s) 116, 345, 419
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 4 cut(s) 375, 448, 578, 1005
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 3 cut(s) 227, 478, 830
MmeI TCCRAC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 576
MroNI GCCGGC 1 cut(s) 883
MscI TGGCCA 1 cut(s) 576
MseI TTAA 2 cut(s) 912, 1008
MslI CAYNNNNRTG 1 cut(s) 941
Msp20I TGGCCA 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 3 cut(s) 325, 346, 884
MspR9I CCNGG 3 cut(s) 325, 666, 752
MunI CAATTG 2 cut(s) 375, 448
MvaI CCWGG 2 cut(s) 666, 752
MvnI CGCG 2 cut(s) 312, 314
MwoI GCNNNNNNNGC 7 cut(s) 161, 167, 176, 488, 503, 527, 668
NaeI GCCGGC 1 cut(s) 885
NciI CCSGG 1 cut(s) 325
NcoI CCATGG 1 cut(s) 597
NdeII GATC 5 cut(s) 330, 583, 703, 981, 1000
NgoMIV GCCGGC 1 cut(s) 883
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 5 cut(s) 259, 601, 619, 693, 1029
NlaIV GGNNCC 3 cut(s) 445, 663, 695
NmeAIII GCCGAG 1 cut(s) 229
NsbI TGCGCA 1 cut(s) 935
NspI RCATGY 3 cut(s) 259, 619, 1029
PaeR7I CTCGAG 2 cut(s) 818, 833
PceI AGGCCT 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PdiI GCCGGC 1 cut(s) 885
PfeI GAWTC 2 cut(s) 892, 990
PfoI TCCNGGA 1 cut(s) 750
PkrI GCNGC 9 cut(s) 52, 106, 163, 286, 316, 505, 508, 661, 732
PleI GAGTC 3 cut(s) 226, 477, 829
PmaCI CACGTG 1 cut(s) 75
PmlI CACGTG 1 cut(s) 75
PpsI GAGTC 3 cut(s) 226, 477, 829
Ppu21I YACGTR 1 cut(s) 75
PpuMI RGGWCCY 1 cut(s) 238
Psp124BI GAGCTC 2 cut(s) 116, 419
Psp5II RGGWCCY 1 cut(s) 238
Psp6I CCWGG 2 cut(s) 664, 750
PspCI CACGTG 1 cut(s) 75
PspGI CCWGG 2 cut(s) 664, 750
PspN4I GGNNCC 3 cut(s) 445, 663, 695
PspPI GGNCC 7 cut(s) 39, 238, 304, 316, 444, 489, 594
PspPPI RGGWCCY 1 cut(s) 238
PspXI VCTCGAGB 2 cut(s) 818, 833
PstNI CAGNNNCTG 1 cut(s) 484
PsuI RGATCY 2 cut(s) 703, 1000
RsaI GTAC 1 cut(s) 477
RsaNI GTAC 1 cut(s) 476
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 2 cut(s) 116, 419
SaqAI TTAA 2 cut(s) 912, 1008
SatI GCNGC 9 cut(s) 51, 105, 162, 285, 315, 504, 507, 660, 731
Sau3AI GATC 5 cut(s) 330, 583, 703, 981, 1000
Sau96I GGNCC 7 cut(s) 39, 238, 304, 316, 444, 489, 594
SchI GAGTC 3 cut(s) 227, 478, 830
ScrFI CCNGG 3 cut(s) 325, 666, 752
SduI GDGCHC 3 cut(s) 116, 345, 419
SfaNI GCATC 3 cut(s) 14, 538, 539
Sfr274I CTCGAG 2 cut(s) 818, 833
SinI GGWCC 4 cut(s) 39, 238, 304, 594
SlaI CTCGAG 2 cut(s) 818, 833
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 4 cut(s) 510, 809, 818, 833
SmoI CTYRAG 4 cut(s) 510, 809, 818, 833
SpeI ACTAGT 1 cut(s) 682
Sse9I AATT 4 cut(s) 375, 448, 578, 1005
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 8 cut(s) 33, 107, 138, 161, 285, 310, 314, 659
SspMI CTAG 4 cut(s) 432, 518, 554, 683
SstI GAGCTC 2 cut(s) 116, 419
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 323, 664, 750
StyI CCWWGG 3 cut(s) 279, 597, 985
TaiI ACGT 2 cut(s) 77, 294
TaqI TCGA 5 cut(s) 116, 333, 652, 819, 834
TasI AATT 4 cut(s) 375, 448, 578, 1005
TauI GCSGC 4 cut(s) 164, 287, 317, 662
TfiI GAWTC 2 cut(s) 892, 990
Tru1I TTAA 2 cut(s) 912, 1008
Tru9I TTAA 2 cut(s) 912, 1008
TscAI CASTG 3 cut(s) 162, 186, 894
TseI GCWGC 5 cut(s) 50, 104, 503, 506, 730
TspDTI ATGAA 1 cut(s) 959
TspGWI ACGGA 2 cut(s) 114, 852
TspRI CASTG 3 cut(s) 162, 186, 894
VpaK11BI GGWCC 4 cut(s) 39, 238, 304, 594
XceI RCATGY 3 cut(s) 259, 619, 1029
XcmI CCANNNNNNNNNTGG 1 cut(s) 704
XhoI CTCGAG 2 cut(s) 818, 833
XspI CTAG 4 cut(s) 432, 518, 554, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.