Rmu_sc0002724.1_g000001

ABC transporter D family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002724.1
OG0006235
Physical Location & Seq
Reverse (-)
4688 .. 21904
17217 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4812 bp
atgccttcgcttcagctgttgcaattaactgagcatggtcggagtttcattgcatcaaggaggaaaaccttgcttcttgctactggtattgtagttgctggtggggctgctgcatatgttcaatcgagattaacccataaaaagcacaattcttttggtcaatataatgggctgaatgaaaataaagtgacagagaatgtcgtggcgaatgatcataagaaaaagaaacttccacaaaagagagggggattgaagtctcttcaagtccttgctgcaattcttttgtctgaaatgggccaaatgggtgcgagggacctcttatctttggtcggaatagtggtgttgcgaactgctttgagcaacagacttgcaaaagtccaagggtttttattccgtgcagcttttcttcgacgtgttccattatttgtccggctgatttcagagaatattctattatgtttccttgcctcaactatgcattcaacttcacagtacataacagggaccttaagtctgcggttcagaaaaatattgacaaaacgtatccattcccattattttgagaatattgcatactacaaaatgtcacatgtcgatggtcggataactaatccggaacagcgaattgcaagtgatgtaccaaggttctgttcagagttgagtgaaatcgtacaagatgatttaacagctgttactgatggtctcctttactcttggcggctgtgttcctacaccagtcctaaatatatattctggatattggcatatgtattgggagcaggggggatgataagaaagttctctcctccttttgggacattaatgtccaaagaacaacaattggaaggggagtaccgacagcttcattcacgattaagaacccatgcagaaagtgtggcattctatggtggagaaagtagggaggaatctcatattcagaagaagtttaacacactggttgggcatctgcgtgttgtccttcatgatcattggtggtttggcatgattcaagacttcctgttgaagtatcttggtgccacagttgctgttatattgattattgagcccttcttttcgggcagtctgaggcctgacacttcaactttaggccgcgcagagatgttaagcaacttaagatatcataccagtgtcataatttcacttttccagtctatgggaactctcgcttcaagttcgagaaaactcaatcgtctcagtggttacgctgatcgcattcatgaattaatggtcatatcaagagagctaagtgctgttgatgacaaatcttctgggagtaaaaattgctctagtgaggctgattacattgaatttgctggggtcaagaatattgcatactacaaaatgtcacatgtcgatggtcggataactaatccggaacagcgaattgcaagtgatgtaccaaggttctgttcagagttgagtgaaatcgtacaagatgatttaacagctgttactgatggtctcctttactcttggcggctgtgttcctacaccagtcctaaatatatattctggatattggcatatgtattgggagcaggggggatgataagaaagttctctcctccttttgggacattaatgtccaaagaacaacaattggaaggggagtaccgacagcttcattcacgattaagaacccatgcagaaagtgtggcattctatggtggagaaagtagggaggaatctcatattcagaagaagtttaacacactggttgggcatctgcgtgttgtccttcatgatcattggtggtttggcatgattcaagacttcctgttgaagtatcttggtgccacagttgctgttatattgattattgagcccttcttttcgggcagtctgaggcctgacacttcaactttaggccgcgcagagatgttaagcaacttaagatatcataccagtgtcataatttcacttttccagtctatgggaactctcgcttcaagttcgagaaaactcaatcgtctcagtggttacgctgatcgcattcatgaattaatggtcatatcaagagagctaagtgctgttgatgacaaatcttctgggagtaaaaattgctctagtgaggctgattacattgaatttgctggggtcaaggttgttacccccacggataatgttttggtggataatttgtcacttagggttgaaccaggatctaatcttttgattacaggtccaaatggcagtggaaagagctcgcttttccgagttttaggaggcctgtggcccttggtatctggccatattgtgaaacctggtgttggaactgatctcaacaaagaaattttctatgttccacaaagaccatacacagctgtcggaacacttcgagaccagttgatttatcctcttactgcagatcaagaggtcaaaccacttacacgtggagaaatggcagagctattgagaaatgttgatcttcagtatttattagaccgttatccacctgaggaagagataaattggggtgatgaattatctcttggtgagcaacaaagattggggatggctagacttttctatcataagcccaaatttgcaattcttgatgagtgcacaagtgctgtcacaactgacatggaggaacgattctgtgctaaagttcgagcaatgggaacatcatgcattactatctcgcatcgtccagcactagttgcatttcatgatgtagtgctgtccttggatggtgaaggcggttggcgtgttcatgataaaagggaggattctctagtacgtaatgaaggtgggagaagtatgttgaagctttctgaaacgaaccggcagagtgatgcattgacagttcagcgagcattcgcctcgaccaaaaaggattccgcaatctcaaactcaaagtcccagtcatacattgctgaggtgatagcagtttctccctctgccgagcataatgttacagttccgtctttcccacatctccaaagtgctccggcagcactgccattgagagcatctgccatgttcaaagtactgattcccactgttattgataaacaaggagcacaactgcttgcagttgcttttctcgtagtatcgaggacatggatttctgatcgtattgcttcattaaatggaaccactgtgaagtttgttttggaacaagataaggcatcatttattcatctaattggtgttagcgtacttcagagtgcggcatcttctttcattgcaccttctataaggcacttaaaagctaggctggctcttgggtggaggactcgtttgactcaacatttacttaaaaactacttgagaaataatgcgttctataaggtattcaacatgtcaagcaaaaacatagacgcagatcagagagttacacaggaccttgaaaagttaaccactgacttgtcgggattggttactggattggtaaagccatctgtggacattctttggttcacctggagaatgaagcttttgactggtcagagaggagtaactatattgtacgcgtatatgttactgggtcttggtttgctcagggctgctaccccagaatttggtgatttgacaagtcgtcaacaacaacttgaaggaacctttaggtttatgcatgaaagactgcgtgctcatgctgaatctgttgctttctttgggggtggttatagagagaaagctatggttgaatcaaagttcaaggagctactccatcactcctcgtctcttttgaagaagcaatggttattcggtattctagatgattttatcacgaaacaactcccacataatgtgacttggggattgagcttgctatatgccattgaacacaagggagaccgagcattaatatctactcaaggtgagctggcacatgcattgcgtttcttggcatctgtggtatctcaaagctttttagctttcggtgacattcttgaattgcatagaaaattcctggaactctctggcagcatcaacagggtttttgaacttgaagagcttttagaagctgcacaatctgtggttggctattctggggattctgaagctggtaactcgtcatcatctgaaagggaaggttttcaatttgaagatgccattaacttctctgaagttgatatcataaccccgtcacggaaattgttggctaggaaattaacatgtgatatagtacctgggaaaagcttgcttgttactggtccaaacgggagtggaaaaagttctgtgtttagagttcttagaggtctttggcccatcatgagtggaagaatcactagaccatctcaagatattaacggagttagtagagatattggatctggttgcggagttttttatgttcctcagcgaccttacacatgcttgggaacactcagggatcaaattatatatcctctctcttttgatgaggcggagaaaagggcattgaagttgtatcaagaaggtggaaaatttgctgacagcacaaacattctggacatgtgtttgggaactattctggagaatgtccgattaagttatcttttggaaagggaggaaggtggttgggatgctaatctcaattgggaggacactctctctctcggagaacagcagagattaggcatggcacgcttattttttcacaaaccaaaattcgccatccttgatgaatgcactaatgctacaagtgttgatgttgaagaacaactctatcggcttgcaaaagacatgggtatcacagttgttacctcctcacaacgtcctgctctaataccattccattccctggaattacggcttattgatggtgagggtaactgggagcttcgatcaataaagcaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006629 GO:0008150 GO:0008152 GO:0009056 GO:0016042 GO:0044238 GO:0071704 GO:1901575
KEGG Pathways
Metabolic & Signaling
ko02010 ko04146 map02010 map04146
Pfam Domains
Protein Families
ABC_membrane_2 ABC_tran

Protein Analysis

1603

Amino Acids

179.46

Weight (kDa)

8.97

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane_2 PF06472 90 - 358 1.3e-87 ABC transporter transmembrane region 2
ABC_membrane_2 PF06472 448 - 618 3.1e-60 ABC transporter transmembrane region 2
ABC_tran PF00005 719 - 864 7.8e-20 ABC transporter
ABC_membrane_2 PF06472 1002 - 1268 3.2e-81 ABC transporter transmembrane region 2
ABC_tran PF00005 1380 - 1549 7.8e-15 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 823, 1603
AccB1I GGYRCC 2 cut(s) 1034, 1814
AccB7I CCANNNNNTGG 4 cut(s) 1174, 1954, 3548, 4753
AccII CGCG 3 cut(s) 1113, 1893, 3500
AccIII TCCGGA 2 cut(s) 613, 1393
AclWI GGATC 3 cut(s) 2191, 4309, 4371
AcoI YGGCCR 1 cut(s) 2269
AcsI RAATTY 8 cut(s) 1328, 2108, 2313, 2564, 3545, 3935, 4436, 4619
AcuI CTGAAG 4 cut(s) 2435, 3173, 4050, 4116
AcvI CACGTG 1 cut(s) 2414
AfiI CCNNNNNNNGG 8 cut(s) 812, 1174, 1592, 1954, 2291, 3548, 4119, 4753
AflII CTTAAG 3 cut(s) 508, 1132, 1912
AflIII ACRYGT 8 cut(s) 412, 589, 1369, 2411, 3327, 3498, 4145, 4464
AhdI GACNNNNNGTC 2 cut(s) 510, 3564
AhlI ACTAGT 1 cut(s) 2680
AjiI CACGTC 1 cut(s) 413
AjnI CCWGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
AjuI GAANNNNNNNTTGG 8 cut(s) 634, 666, 1414, 1446, 2496, 2528, 3122, 3154
Alw21I GWGCWC 5 cut(s) 2228, 2588, 2974, 3049, 3619
Alw26I GTCTC 8 cut(s) 261, 707, 1217, 1487, 1997, 2355, 3714, 3816
Alw44I GTGCAC 1 cut(s) 2584
AlwI GGATC 3 cut(s) 2191, 4309, 4371
AlwNI CAGNNNCTG 2 cut(s) 1046, 1826
Aor13HI TCCGGA 2 cut(s) 613, 1393
AoxI GGCC 9 cut(s) 295, 1088, 1108, 1868, 1888, 2248, 2255, 2269, 4235
ApaLI GTGCAC 1 cut(s) 2584
ApeKI GCWGC 8 cut(s) 107, 110, 272, 398, 2978, 3533, 3954, 3995
ApoI RAATTY 8 cut(s) 1328, 2108, 2313, 2564, 3545, 3935, 4436, 4619
ArsI GACNNNNNNTTYG 4 cut(s) 2867, 2873, 2899, 2905
AseI ATTAAT 5 cut(s) 821, 1244, 1601, 2024, 3833
Asp700I GAANNNNTTC 2 cut(s) 4065, 4204
AspLEI GCGC 2 cut(s) 1115, 1895
AspS9I GGNCC 8 cut(s) 295, 313, 504, 2204, 2256, 3370, 4184, 4236
AsuHPI GGTGA 9 cut(s) 2510, 2528, 2729, 2917, 3439, 3563, 3860, 3923, 4787
AvaII GGWCC 5 cut(s) 313, 504, 2204, 3370, 4184
AxyI CCTNAGG 1 cut(s) 2478
BaeGI GKGCMC 1 cut(s) 2588
BaeI ACNNNNGTAYC 2 cut(s) 4684, 4717
BalI TGGCCA 1 cut(s) 2271
BanI GGYRCC 2 cut(s) 1034, 1814
BanII GRGCYC 3 cut(s) 1068, 1848, 2228
BbrPI CACGTG 1 cut(s) 2414
Bbv12I GWGCWC 5 cut(s) 2228, 2588, 2974, 3049, 3619
BbvCI CCTCAGC 2 cut(s) 2901, 4329
BbvI GCAGC 8 cut(s) 94, 97, 259, 410, 2990, 3520, 3966, 3982
BceAI ACGGC 1 cut(s) 4778
BcgI CGANNNNNNTGC 2 cut(s) 2829, 2863
BciT130I CCWGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
BciVI GTATCC 1 cut(s) 554
BclI TGATCA 3 cut(s) 211, 985, 1765
BcoDI GTCTC 8 cut(s) 261, 707, 1217, 1487, 1997, 2355, 3714, 3816
BcuI ACTAGT 1 cut(s) 2680
BfaI CTAG 9 cut(s) 1308, 2088, 2541, 2681, 2759, 3240, 3743, 4134, 4260
BfmI CTRYAG 1 cut(s) 2385
BfrI CTTAAG 3 cut(s) 508, 1132, 1912
BfuI GTATCC 1 cut(s) 554
BmcAI AGTACT 1 cut(s) 3015
Bme1390I CCNGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
Bme18I GGWCC 5 cut(s) 313, 504, 2204, 3370, 4184
BmeRI GACNNNNNGTC 2 cut(s) 510, 3564
BmgBI CACGTC 1 cut(s) 413
BmgT120I GGNCC 8 cut(s) 295, 313, 504, 2204, 2256, 3370, 4184, 4236
BmiI GGNNCC 6 cut(s) 314, 505, 1036, 1816, 3121, 3586
BmrFI CCNGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
BmrI ACTGGG 3 cut(s) 2881, 3521, 4795
BmuI ACTGGG 3 cut(s) 2881, 3521, 4795
BpmI CTGGAG 2 cut(s) 3472, 4505
Bpu10I CCTNAGC 3 cut(s) 2901, 3527, 4329
BpuEI CTTGAG 3 cut(s) 3316, 3828, 4254
BsaAI YACGTR 2 cut(s) 2414, 2765
BsaBI GATNNNNATC 1 cut(s) 4539
BsaI GGTCTC 4 cut(s) 707, 1487, 2355, 3816
BsaJI CCNNGG 8 cut(s) 379, 641, 1421, 2136, 2259, 2709, 4160, 4752
BsaWI WCCGGW 2 cut(s) 613, 1393
BsaXI ACNNNNNCTCC 2 cut(s) 3250, 3280
Bsc4I CCNNNNNNNGG 8 cut(s) 812, 1174, 1592, 1954, 2291, 3548, 4119, 4753
Bse118I RCCGGY 1 cut(s) 2808
Bse21I CCTNAGG 1 cut(s) 2478
Bse3DI GCAATG 6 cut(s) 48, 2646, 2895, 3210, 3731, 3863
Bse8I GATNNNNATC 1 cut(s) 4539
BseAI TCCGGA 2 cut(s) 613, 1393
BseBI CCWGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
BseDI CCNNGG 8 cut(s) 379, 641, 1421, 2136, 2259, 2709, 4160, 4752
BseGI GGATG 6 cut(s) 792, 1572, 2541, 2719, 4540, 4626
BseJI GATNNNNATC 1 cut(s) 4539
BseLI CCNNNNNNNGG 8 cut(s) 812, 1174, 1592, 1954, 2291, 3548, 4119, 4753
BseMI GCAATG 6 cut(s) 48, 2646, 2895, 3210, 3731, 3863
BseRI GAGGAG 5 cut(s) 795, 1575, 3495, 3694, 4708
BseSI GKGCMC 1 cut(s) 2588
BseXI GCAGC 8 cut(s) 94, 97, 259, 410, 2990, 3520, 3966, 3982
BseYI CCCAGC 2 cut(s) 1334, 2114
BsgI GTGCAG 2 cut(s) 417, 3981
Bsh1236I CGCG 3 cut(s) 1113, 1893, 3500
BshFI GGCC 9 cut(s) 297, 1090, 1110, 1870, 1890, 2250, 2257, 2271, 4237
BshNI GGYRCC 2 cut(s) 1034, 1814
BsiHKAI GWGCWC 5 cut(s) 2228, 2588, 2974, 3049, 3619
BsiSI CCGG 5 cut(s) 430, 614, 1394, 2809, 2975
BslFI GGGAC 5 cut(s) 326, 517, 829, 1609, 2869
BslI CCNNNNNNNGG 8 cut(s) 812, 1174, 1592, 1954, 2291, 3548, 4119, 4753
BsmAI GTCTC 8 cut(s) 261, 707, 1217, 1487, 1997, 2355, 3714, 3816
BsmBI CGTCTC 3 cut(s) 1217, 1997, 3714
BsmFI GGGAC 5 cut(s) 326, 517, 829, 1609, 2869
BsmI GAATGC 7 cut(s) 478, 899, 1233, 1679, 2013, 2840, 4643
BsnI GGCC 9 cut(s) 297, 1090, 1110, 1870, 1890, 2250, 2257, 2271, 4237
Bso31I GGTCTC 4 cut(s) 707, 1487, 2355, 3816
Bsp1286I GDGCHC 7 cut(s) 1068, 1848, 2228, 2588, 2974, 3049, 3619
Bsp13I TCCGGA 2 cut(s) 613, 1393
BspANI GGCC 9 cut(s) 297, 1090, 1110, 1870, 1890, 2250, 2257, 2271, 4237
BspEI TCCGGA 2 cut(s) 613, 1393
BspFNI CGCG 3 cut(s) 1113, 1893, 3500
BspHI TCATGA 7 cut(s) 982, 1237, 1762, 2017, 2692, 2737, 4242
BspLI GGNNCC 6 cut(s) 314, 505, 1036, 1816, 3121, 3586
BspMAI CTGCAG 1 cut(s) 2389
BspPI GGATC 3 cut(s) 2191, 4309, 4371
BspQI GCTCTTC 1 cut(s) 3975
BspT107I GGYRCC 2 cut(s) 1034, 1814
BspTI CTTAAG 3 cut(s) 508, 1132, 1912
BspTNI GGTCTC 4 cut(s) 707, 1487, 2355, 3816
BsrDI GCAATG 6 cut(s) 48, 2646, 2895, 3210, 3731, 3863
BsrFI RCCGGY 1 cut(s) 2808
BssAI RCCGGY 1 cut(s) 2808
BssECI CCNNGG 8 cut(s) 379, 641, 1421, 2136, 2259, 2709, 4160, 4752
BssT1I CCWWGG 5 cut(s) 379, 641, 1421, 2259, 2709
Bst2UI CCWGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
Bst4CI ACNGT 9 cut(s) 492, 1042, 1822, 2468, 2830, 2944, 3028, 3127, 4708
Bst6I CTCTTC 3 cut(s) 264, 2478, 3975
BstAFI CTTAAG 3 cut(s) 508, 1132, 1912
BstAPI GCANNNNNTGC 1 cut(s) 2684
BstBAI YACGTR 2 cut(s) 2414, 2765
BstDSI CCRYGG 1 cut(s) 2136
BstF5I GGATG 6 cut(s) 792, 1572, 2541, 2719, 4540, 4626
BstFNI CGCG 3 cut(s) 1113, 1893, 3500
BstHHI GCGC 2 cut(s) 1115, 1895
BstMAI GTCTC 8 cut(s) 261, 707, 1217, 1487, 1997, 2355, 3714, 3816
BstMWI GCNNNNNNNGC 7 cut(s) 104, 1043, 1823, 2684, 2978, 3245, 4596
BstNI CCWGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
BstNSI RCATGY 7 cut(s) 593, 1373, 3331, 3863, 4149, 4347, 4468
BstSCI CCNGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
BstSFI CTRYAG 1 cut(s) 2385
BstSLI GKGCMC 1 cut(s) 2588
BstSNI TACGTA 1 cut(s) 2765
BstUI CGCG 3 cut(s) 1113, 1893, 3500
BstV1I GCAGC 8 cut(s) 94, 97, 259, 410, 2990, 3520, 3966, 3982
BstX2I RGATCY 2 cut(s) 2183, 4301
BstYI RGATCY 2 cut(s) 2183, 4301
Bsu36I CCTNAGG 1 cut(s) 2478
BsuI GTATCC 1 cut(s) 554
BsuRI GGCC 9 cut(s) 297, 1090, 1110, 1870, 1890, 2250, 2257, 2271, 4237
BtgI CCRYGG 1 cut(s) 2136
BtrI CACGTC 1 cut(s) 413
BtsCI GGATG 6 cut(s) 792, 1572, 2541, 2719, 4540, 4626
BtsI GCAGTG 2 cut(s) 2221, 2981
CaiI CAGNNNCTG 2 cut(s) 1046, 1826
CciI TCATGA 7 cut(s) 982, 1237, 1762, 2017, 2692, 2737, 4242
CfoI GCGC 2 cut(s) 1115, 1895
Cfr10I RCCGGY 1 cut(s) 2808
Cfr13I GGNCC 8 cut(s) 295, 313, 504, 2204, 2256, 3370, 4184, 4236
CseI GACGC 1 cut(s) 3356
CsiI ACCWGGT 1 cut(s) 2284
CspCI CAANNNNNGTGG 2 cut(s) 3013, 3048
DrdI GACNNNNNNGTC 2 cut(s) 823, 1603
DriI GACNNNNNGTC 2 cut(s) 510, 3564
DseDI GACNNNNNNGTC 2 cut(s) 823, 1603
EaeI YGGCCR 1 cut(s) 2269
Eam1104I CTCTTC 3 cut(s) 264, 2478, 3975
Eam1105I GACNNNNNGTC 2 cut(s) 510, 3564
EarI CTCTTC 3 cut(s) 264, 2478, 3975
EciI GGCGGA 1 cut(s) 4412
Ecl136II GAGCTC 1 cut(s) 2226
Eco105I TACGTA 1 cut(s) 2765
Eco130I CCWWGG 5 cut(s) 379, 641, 1421, 2259, 2709
Eco147I AGGCCT 3 cut(s) 1090, 1870, 2250
Eco24I GRGCYC 3 cut(s) 1068, 1848, 2228
Eco31I GGTCTC 4 cut(s) 707, 1487, 2355, 3816
Eco32I GATATC 3 cut(s) 1139, 1919, 4105
Eco47I GGWCC 5 cut(s) 313, 504, 2204, 3370, 4184
Eco53kI GAGCTC 1 cut(s) 2226
Eco57I CTGAAG 4 cut(s) 2435, 3173, 4050, 4116
Eco72I CACGTG 1 cut(s) 2414
Eco81I CCTNAGG 1 cut(s) 2478
EcoICRI GAGCTC 1 cut(s) 2226
EcoO109I RGGNCCY 3 cut(s) 313, 504, 3370
EcoRII CCWGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
EcoRV GATATC 3 cut(s) 1139, 1919, 4105
EcoT14I CCWWGG 5 cut(s) 379, 641, 1421, 2259, 2709
EcoT22I ATGCAT 5 cut(s) 480, 2657, 2824, 3603, 3865
EcoT38I GRGCYC 3 cut(s) 1068, 1848, 2228
ErhI CCWWGG 5 cut(s) 379, 641, 1421, 2259, 2709
Esp3I CGTCTC 3 cut(s) 1217, 1997, 3714
FaqI GGGAC 5 cut(s) 326, 517, 829, 1609, 2869
FauNDI CATATG 3 cut(s) 115, 766, 1546
FbaI TGATCA 3 cut(s) 211, 985, 1765
FokI GGATG 6 cut(s) 799, 1579, 2548, 2726, 4547, 4613
FriOI GRGCYC 3 cut(s) 1068, 1848, 2228
FspBI CTAG 9 cut(s) 1308, 2088, 2541, 2681, 2759, 3240, 3743, 4134, 4260
GlaI GCGC 2 cut(s) 1114, 1894
GsaI CCCAGC 2 cut(s) 1338, 2118
GsuI CTGGAG 2 cut(s) 3472, 4505
HaeIII GGCC 9 cut(s) 297, 1090, 1110, 1870, 1890, 2250, 2257, 2271, 4237
HapII CCGG 5 cut(s) 430, 614, 1394, 2809, 2975
HgaI GACGC 1 cut(s) 3356
HhaI GCGC 2 cut(s) 1115, 1895
Hin6I GCGC 2 cut(s) 1113, 1893
HinP1I GCGC 2 cut(s) 1113, 1893
HincII GTYRAC 2 cut(s) 3384, 3569
HindII GTYRAC 2 cut(s) 3384, 3569
HindIII AAGCTT 4 cut(s) 2792, 3461, 3895, 4168
HpaI GTTAAC 1 cut(s) 3384
HpaII CCGG 5 cut(s) 430, 614, 1394, 2809, 2975
HphI GGTGA 9 cut(s) 2510, 2528, 2729, 2917, 3439, 3563, 3860, 3923, 4787
Hpy166II GTNNAC 5 cut(s) 2586, 3384, 3433, 3447, 3569
Hpy8I GTNNAC 5 cut(s) 2586, 3384, 3433, 3447, 3569
Hpy99I CGWCG 1 cut(s) 414
HpyCH4III ACNGT 9 cut(s) 492, 1042, 1822, 2468, 2830, 2944, 3028, 3127, 4708
HpyCH4IV ACGT 5 cut(s) 412, 541, 2413, 2764, 4726
HpyF10VI GCNNNNNNNGC 7 cut(s) 104, 1043, 1823, 2684, 2978, 3245, 4596
HpySE526I ACGT 5 cut(s) 412, 541, 2413, 2764, 4726
HspAI GCGC 2 cut(s) 1113, 1893
Kpn2I TCCGGA 2 cut(s) 613, 1393
Ksp22I TGATCA 3 cut(s) 211, 985, 1765
KspAI GTTAAC 1 cut(s) 3384
LguI GCTCTTC 1 cut(s) 3975
LmnI GCTCC 6 cut(s) 776, 1556, 2977, 3044, 3688, 4789
Lsp1109I GCAGC 8 cut(s) 94, 97, 259, 410, 2990, 3520, 3966, 3982
MabI ACCWGGT 1 cut(s) 2284
MaeI CTAG 9 cut(s) 1308, 2088, 2541, 2681, 2759, 3240, 3743, 4134, 4260
MaeII ACGT 5 cut(s) 412, 541, 2413, 2764, 4726
MfeI CAATTG 3 cut(s) 839, 1619, 4546
MflI RGATCY 2 cut(s) 2183, 4301
MhlI GDGCHC 7 cut(s) 1068, 1848, 2228, 2588, 2974, 3049, 3619
MlsI TGGCCA 1 cut(s) 2271
MluI ACGCGT 1 cut(s) 3498
MluNI TGGCCA 1 cut(s) 2271
MlyI GAGTC 2 cut(s) 3256, 3265
MmeI TCCRAC 6 cut(s) 20, 310, 581, 1361, 2272, 2329
Mox20I TGGCCA 1 cut(s) 2271
Mph1103I ATGCAT 5 cut(s) 480, 2657, 2824, 3603, 3865
MroI TCCGGA 2 cut(s) 613, 1393
MroXI GAANNNNTTC 2 cut(s) 4065, 4204
MscI TGGCCA 1 cut(s) 2271
MslI CAYNNNNRTG 6 cut(s) 594, 969, 1146, 1374, 1749, 1926
Msp20I TGGCCA 1 cut(s) 2271
MspA1I CMGCKG 4 cut(s) 16, 689, 1469, 2345
MspCI CTTAAG 3 cut(s) 508, 1132, 1912
MspI CCGG 5 cut(s) 430, 614, 1394, 2809, 2975
MspR9I CCNGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
MunI CAATTG 3 cut(s) 839, 1619, 4546
Mva1269I GAATGC 7 cut(s) 478, 899, 1233, 1679, 2013, 2840, 4643
MvaI CCWGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
MvnI CGCG 3 cut(s) 1113, 1893, 3500
MwoI GCNNNNNNNGC 7 cut(s) 104, 1043, 1823, 2684, 2978, 3245, 4596
NdeI CATATG 3 cut(s) 115, 766, 1546
NlaIV GGNNCC 6 cut(s) 314, 505, 1036, 1816, 3121, 3586
NmeAIII GCCGAG 1 cut(s) 2953
NmuCI GTSAC 8 cut(s) 187, 585, 1365, 2163, 2596, 3778, 3911, 4116
NsiI ATGCAT 5 cut(s) 480, 2657, 2824, 3603, 3865
NspI RCATGY 7 cut(s) 593, 1373, 3331, 3863, 4149, 4347, 4468
PagI TCATGA 7 cut(s) 982, 1237, 1762, 2017, 2692, 2737, 4242
PceI AGGCCT 3 cut(s) 1090, 1870, 2250
PciI ACATGT 5 cut(s) 589, 1369, 3327, 4145, 4464
PciSI GCTCTTC 1 cut(s) 3975
PctI GAATGC 7 cut(s) 478, 899, 1233, 1679, 2013, 2840, 4643
PdmI GAANNNNTTC 2 cut(s) 4065, 4204
PflMI CCANNNNNTGG 4 cut(s) 1174, 1954, 3548, 4753
PfoI TCCNGGA 1 cut(s) 3939
PleI GAGTC 2 cut(s) 3256, 3265
PmaCI CACGTG 1 cut(s) 2414
PmlI CACGTG 1 cut(s) 2414
PpsI GAGTC 2 cut(s) 3256, 3265
Ppu21I YACGTR 2 cut(s) 2414, 2765
PpuMI RGGWCCY 3 cut(s) 313, 504, 3370
PscI ACATGT 5 cut(s) 589, 1369, 3327, 4145, 4464
PshBI ATTAAT 5 cut(s) 821, 1244, 1601, 2024, 3833
Psp124BI GAGCTC 1 cut(s) 2228
Psp5II RGGWCCY 3 cut(s) 313, 504, 3370
Psp6I CCWGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
PspCI CACGTG 1 cut(s) 2414
PspFI CCCAGC 2 cut(s) 1334, 2114
PspGI CCWGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
PspN4I GGNNCC 6 cut(s) 314, 505, 1036, 1816, 3121, 3586
PspPI GGNCC 8 cut(s) 295, 313, 504, 2204, 2256, 3370, 4184, 4236
PspPPI RGGWCCY 3 cut(s) 313, 504, 3370
PstI CTGCAG 1 cut(s) 2389
PstNI CAGNNNCTG 2 cut(s) 1046, 1826
PsuI RGATCY 2 cut(s) 2183, 4301
PvuII CAGCTG 4 cut(s) 16, 689, 1469, 2345
RseI CAYNNNNRTG 6 cut(s) 594, 969, 1146, 1374, 1749, 1926
SacI GAGCTC 1 cut(s) 2228
SapI GCTCTTC 1 cut(s) 3975
Sau96I GGNCC 8 cut(s) 295, 313, 504, 2204, 2256, 3370, 4184, 4236
ScaI AGTACT 1 cut(s) 3015
SchI GAGTC 2 cut(s) 3256, 3265
ScrFI CCNGG 6 cut(s) 2181, 2286, 3451, 3941, 4161, 4754
SduI GDGCHC 7 cut(s) 1068, 1848, 2228, 2588, 2974, 3049, 3619
SexAI ACCWGGT 1 cut(s) 2284
SfcI CTRYAG 1 cut(s) 2385
SinI GGWCC 5 cut(s) 313, 504, 2204, 3370, 4184
SmiMI CAYNNNNRTG 6 cut(s) 594, 969, 1146, 1374, 1749, 1926
SmlI CTYRAG 6 cut(s) 508, 1132, 1912, 3295, 3843, 4269
SmoI CTYRAG 6 cut(s) 508, 1132, 1912, 3295, 3843, 4269
SnaBI TACGTA 1 cut(s) 2765
SpeI ACTAGT 1 cut(s) 2680
SseBI AGGCCT 3 cut(s) 1090, 1870, 2250
SspI AATATT 4 cut(s) 448, 531, 568, 1348
SspMI CTAG 9 cut(s) 1308, 2088, 2541, 2681, 2759, 3240, 3743, 4134, 4260
SstI GAGCTC 1 cut(s) 2228
StuI AGGCCT 3 cut(s) 1090, 1870, 2250
StyD4I CCNGG 6 cut(s) 2179, 2284, 3449, 3939, 4159, 4752
StyI CCWWGG 5 cut(s) 379, 641, 1421, 2259, 2709
TaaI ACNGT 9 cut(s) 492, 1042, 1822, 2468, 2830, 2944, 3028, 3127, 4708
TaiI ACGT 5 cut(s) 415, 544, 2416, 2767, 4729
TaqII GACCGA 1 cut(s) 3840
TatI WGTACW 2 cut(s) 492, 3013
TauI GCSGC 5 cut(s) 721, 1113, 1501, 1893, 3200
TseFI GTSAC 8 cut(s) 187, 585, 1365, 2163, 2596, 3778, 3911, 4116
TseI GCWGC 8 cut(s) 107, 110, 272, 398, 2978, 3533, 3954, 3995
Tsp45I GTSAC 8 cut(s) 187, 585, 1365, 2163, 2596, 3778, 3911, 4116
TspGWI ACGGA 5 cut(s) 383, 2153, 2937, 4135, 4296
Van91I CCANNNNNTGG 4 cut(s) 1174, 1954, 3548, 4753
Vha464I CTTAAG 3 cut(s) 508, 1132, 1912
VneI GTGCAC 1 cut(s) 2584
VpaK11BI GGWCC 5 cut(s) 313, 504, 2204, 3370, 4184
VspI ATTAAT 5 cut(s) 821, 1244, 1601, 2024, 3833
XapI RAATTY 8 cut(s) 1328, 2108, 2313, 2564, 3545, 3935, 4436, 4619
XbaI TCTAGA 1 cut(s) 3742
XceI RCATGY 7 cut(s) 593, 1373, 3331, 3863, 4149, 4347, 4468
XmnI GAANNNNTTC 2 cut(s) 4065, 4204
XspI CTAG 9 cut(s) 1308, 2088, 2541, 2681, 2759, 3240, 3743, 4134, 4260
ZrmI AGTACT 1 cut(s) 3015
Zsp2I ATGCAT 5 cut(s) 480, 2657, 2824, 3603, 3865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.