Rmu_sc0002729.1_g000001

tRNA pseudouridine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002729.1
OG0006106
Physical Location & Seq
Forward (+)
1 .. 3681
3681 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 901 bp
tgttgttgagcaatctcttgggaaatttgttgatgacaagaaagcccagatgctgaaagacaaagggttgccattggaaggggctgctactgtcgccggacggaccgataaaggagtcacagcccttaatcaagtttgttctttctatacttggagaaaggatgtgcaatctcaagatattgcagaagctataagtagtgcagcaccaggaaaactcaggacagtatcagttactgaggtatcacgtgtattccatccaaatttttctgccaaatggaggcgttatttgtatatatttccatttaatgatgatgaggaggacagggaacaaagcaatgagactggagagaatactcaaaaatttaagattaatgataatcattatgagcatgaagttgtatctgaaaaatgcaatggggaaaatgttggaaacttaatcatcactgatgacgtagagtttgaaactgagaagaagccacggatgttcagcgtaagcagggtgaacaaacttttacagaaactagaaggaaagttattatcatataagctatttgcacgcgataccaaagcatccagaaatgagggtccaccaacagagtgttttgtcttccatgctcgagcaagagagtacagattaccgtgttcagagcatgaaaaagggagaagggtcatgtgtatcgaactggttgccaaccgattcctacgcaggatggttcgggttcttgtggcaacctcaattagggaagccactgcaggtgcagatgaggacgcattgctaaaactgatggatgcaacatgcagacgtgctactgcccccccagctccacccgatggtttatgccttgttgatgttggttatgaagaattcgacccgctagattgcctcatctcgaaggagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PseudoU_synth_1

Protein Analysis

299

Amino Acids

33.85

Weight (kDa)

6.09

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PseudoU_synth_1 PF01416 221 - 287 2.4e-08 tRNA pseudouridine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 744
Acc36I ACCTGC 1 cut(s) 744
AccB7I CCANNNNNTGG 1 cut(s) 831
AccII CGCG 1 cut(s) 559
AciI CCGC 1 cut(s) 873
AcsI RAATTY 4 cut(s) 24, 260, 360, 864
AcvI CACGTG 1 cut(s) 246
AfaI GTAC 1 cut(s) 630
AfiI CCNNNNNNNGG 4 cut(s) 78, 277, 739, 831
AflIII ACRYGT 1 cut(s) 245
AgsI TTSAA 1 cut(s) 462
AjiI CACGTC 1 cut(s) 804
AjnI CCWGG 1 cut(s) 206
AluBI AGCT 3 cut(s) 189, 548, 822
AluI AGCT 3 cut(s) 189, 548, 822
Alw26I GTCTC 1 cut(s) 333
AlwNI CAGNNNCTG 2 cut(s) 53, 234
Ama87I CYCGRG 1 cut(s) 616
ApeKI GCWGC 2 cut(s) 84, 201
ApoI RAATTY 4 cut(s) 24, 260, 360, 864
ArsI GACNNNNNNTTYG 2 cut(s) 441, 473
AseI ATTAAT 1 cut(s) 370
AspS9I GGNCC 2 cut(s) 103, 585
AsuHPI GGTGA 1 cut(s) 512
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 2 cut(s) 103, 585
BaeI ACNNNNGTAYC 2 cut(s) 223, 256
BbrPI CACGTG 1 cut(s) 246
BbsI GAAGAC 1 cut(s) 599
BbvI GCAGC 2 cut(s) 71, 213
BccI CCATC 4 cut(s) 262, 704, 779, 825
BcgI CGANNNNNNTGC 2 cut(s) 669, 703
BciT130I CCWGG 1 cut(s) 208
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 2 cut(s) 522, 876
BfmI CTRYAG 1 cut(s) 751
BfuAI ACCTGC 1 cut(s) 744
BisI GCNGC 2 cut(s) 85, 202
BlsI GCNGC 2 cut(s) 86, 203
Bme1390I CCNGG 1 cut(s) 208
Bme18I GGWCC 2 cut(s) 103, 585
BmeT110I CYCGRG 1 cut(s) 616
BmgBI CACGTC 1 cut(s) 804
BmgT120I GGNCC 2 cut(s) 103, 585
BmiI GGNNCC 1 cut(s) 586
BmrFI CCNGG 1 cut(s) 208
BmsI GCATC 3 cut(s) 40, 579, 779
BpiI GAAGAC 1 cut(s) 599
BpmI CTGGAG 1 cut(s) 364
BpuEI CTTGAG 1 cut(s) 157
BsaAI YACGTR 1 cut(s) 246
BsaJI CCNNGG 1 cut(s) 477
Bsc4I CCNNNNNNNGG 4 cut(s) 78, 277, 739, 831
Bse1I ACTGG 2 cut(s) 347, 688
Bse3DI GCAATG 3 cut(s) 341, 419, 771
BseBI CCWGG 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 6 cut(s) 167, 254, 487, 570, 715, 794
BseLI CCNNNNNNNGG 4 cut(s) 78, 277, 739, 831
BseMI GCAATG 3 cut(s) 341, 419, 771
BseMII CTCAG 3 cut(s) 226, 230, 457
BseNI ACTGG 2 cut(s) 347, 688
BseRI GAGGAG 1 cut(s) 330
BseXI GCAGC 2 cut(s) 71, 213
BseYI CCCAGC 1 cut(s) 818
BsgI GTGCAG 2 cut(s) 220, 778
Bsh1236I CGCG 1 cut(s) 559
BsiHKCI CYCGRG 1 cut(s) 616
BsiSI CCGG 1 cut(s) 97
BslI CCNNNNNNNGG 4 cut(s) 78, 277, 739, 831
BsmAI GTCTC 1 cut(s) 333
BsoBI CYCGRG 1 cut(s) 616
BspACI CCGC 1 cut(s) 873
BspCNI CTCAG 3 cut(s) 227, 229, 458
BspFNI CGCG 1 cut(s) 559
BspLI GGNNCC 1 cut(s) 586
BspMAI CTGCAG 1 cut(s) 755
BspMI ACCTGC 1 cut(s) 744
BsrDI GCAATG 3 cut(s) 341, 419, 771
BsrI ACTGG 2 cut(s) 347, 688
BssECI CCNNGG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 208
Bst4CI ACNGT 3 cut(s) 92, 224, 640
BstBAI YACGTR 1 cut(s) 246
BstC8I GCNNGC 1 cut(s) 557
BstDEI CTNAG 3 cut(s) 216, 235, 466
BstDSI CCRYGG 1 cut(s) 477
BstENI CCTNNNNNAGG 1 cut(s) 737
BstF5I GGATG 6 cut(s) 167, 254, 487, 570, 715, 794
BstFNI CGCG 1 cut(s) 559
BstMAI GTCTC 1 cut(s) 333
BstMWI GCNNNNNNNGC 2 cut(s) 93, 819
BstNI CCWGG 1 cut(s) 208
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 1 cut(s) 206
BstSFI CTRYAG 1 cut(s) 751
BstUI CGCG 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 71, 213
BstV2I GAAGAC 1 cut(s) 599
BtgI CCRYGG 1 cut(s) 477
BtrI CACGTC 1 cut(s) 804
BtsCI GGATG 6 cut(s) 167, 254, 487, 570, 715, 794
BtsI GCAGTG 1 cut(s) 748
BtsIMutI CAGTG 2 cut(s) 442, 748
BveI ACCTGC 1 cut(s) 744
Cac8I GCNNGC 1 cut(s) 557
CaiI CAGNNNCTG 2 cut(s) 53, 234
Cfr13I GGNCC 2 cut(s) 103, 585
CpoI CGGWCCG 1 cut(s) 103
CseI GACGC 1 cut(s) 777
Csp6I GTAC 1 cut(s) 629
CspI CGGWCCG 1 cut(s) 103
CviAII CATG 5 cut(s) 390, 612, 651, 671, 796
CviJI RGCY 8 cut(s) 45, 84, 123, 189, 476, 548, 747, 822
CviKI_1 RGCY 8 cut(s) 45, 84, 123, 189, 476, 548, 747, 822
CviQI GTAC 1 cut(s) 629
DdeI CTNAG 3 cut(s) 216, 235, 466
Eco47I GGWCC 2 cut(s) 103, 585
Eco72I CACGTG 1 cut(s) 246
Eco88I CYCGRG 1 cut(s) 616
EcoNI CCTNNNNNAGG 1 cut(s) 737
EcoRI GAATTC 1 cut(s) 864
EcoRII CCWGG 1 cut(s) 206
FaeI CATG 5 cut(s) 393, 615, 654, 674, 799
FatI CATG 5 cut(s) 389, 611, 650, 670, 795
FauI CCCGC 1 cut(s) 880
Fnu4HI GCNGC 2 cut(s) 85, 202
FokI GGATG 6 cut(s) 174, 241, 494, 557, 722, 801
Fsp4HI GCNGC 2 cut(s) 85, 202
FspBI CTAG 2 cut(s) 522, 876
GluI GCNGC 2 cut(s) 85, 202
GsaI CCCAGC 1 cut(s) 822
GsuI CTGGAG 1 cut(s) 364
HapII CCGG 1 cut(s) 97
HgaI GACGC 1 cut(s) 777
Hin1II CATG 5 cut(s) 393, 615, 654, 674, 799
HinfI GANTC 2 cut(s) 115, 697
HpaII CCGG 1 cut(s) 97
HphI GGTGA 1 cut(s) 512
Hpy166II GTNNAC 2 cut(s) 503, 588
Hpy188I TCNGA 2 cut(s) 404, 647
Hpy188III TCNNGA 4 cut(s) 174, 218, 574, 890
Hpy8I GTNNAC 2 cut(s) 503, 588
HpyAV CCTTC 4 cut(s) 72, 519, 658, 887
HpyCH4III ACNGT 3 cut(s) 92, 224, 640
HpyCH4IV ACGT 3 cut(s) 245, 451, 803
HpyCH4V TGCA 9 cut(s) 167, 183, 201, 412, 555, 753, 759, 792, 799
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 819
HpyF3I CTNAG 3 cut(s) 216, 235, 466
HpySE526I ACGT 3 cut(s) 245, 451, 803
Hsp92II CATG 5 cut(s) 393, 615, 654, 674, 799
LmnI GCTCC 1 cut(s) 827
Lsp1109I GCAGC 2 cut(s) 71, 213
LweI GCATC 3 cut(s) 40, 579, 779
MaeI CTAG 2 cut(s) 522, 876
MaeII ACGT 3 cut(s) 245, 451, 803
MaeIII GTNAC 2 cut(s) 116, 230
MboII GAAGA 3 cut(s) 482, 599, 873
MluCI AATT 5 cut(s) 24, 260, 360, 736, 864
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 407
MnlI CCTC 8 cut(s) 230, 271, 308, 311, 575, 743, 758, 894
MseI TTAA 5 cut(s) 127, 304, 364, 370, 435
MspI CCGG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 208
MvaI CCWGG 1 cut(s) 208
MvnI CGCG 1 cut(s) 559
MwoI GCNNNNNNNGC 2 cut(s) 93, 819
NlaIII CATG 5 cut(s) 393, 615, 654, 674, 799
NlaIV GGNNCC 1 cut(s) 586
NmuCI GTSAC 1 cut(s) 116
NspI RCATGY 1 cut(s) 799
PaeR7I CTCGAG 1 cut(s) 616
PaqCI CACCTGC 1 cut(s) 744
PfeI GAWTC 1 cut(s) 697
PflMI CCANNNNNTGG 1 cut(s) 831
PkrI GCNGC 2 cut(s) 86, 203
PleI GAGTC 1 cut(s) 123
PmaCI CACGTG 1 cut(s) 246
PmlI CACGTG 1 cut(s) 246
PpsI GAGTC 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 246
PshBI ATTAAT 1 cut(s) 370
Psp6I CCWGG 1 cut(s) 206
PspCI CACGTG 1 cut(s) 246
PspFI CCCAGC 1 cut(s) 818
PspGI CCWGG 1 cut(s) 206
PspN4I GGNNCC 1 cut(s) 586
PspPI GGNCC 2 cut(s) 103, 585
PspXI VCTCGAGB 1 cut(s) 616
PstI CTGCAG 1 cut(s) 755
PstNI CAGNNNCTG 2 cut(s) 53, 234
RsaI GTAC 1 cut(s) 630
RsaNI GTAC 1 cut(s) 629
Rsr2I CGGWCCG 1 cut(s) 103
RsrII CGGWCCG 1 cut(s) 103
SaqAI TTAA 5 cut(s) 127, 304, 364, 370, 435
SatI GCNGC 2 cut(s) 85, 202
Sau96I GGNCC 2 cut(s) 103, 585
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 208
SetI ASST 9 cut(s) 191, 241, 248, 454, 550, 735, 758, 806, 824
SfaNI GCATC 3 cut(s) 40, 579, 779
SfcI CTRYAG 1 cut(s) 751
Sfr274I CTCGAG 1 cut(s) 616
SinI GGWCC 2 cut(s) 103, 585
SlaI CTCGAG 1 cut(s) 616
SmlI CTYRAG 2 cut(s) 172, 616
SmoI CTYRAG 2 cut(s) 172, 616
Sse9I AATT 5 cut(s) 24, 260, 360, 736, 864
SsiI CCGC 1 cut(s) 873
SspMI CTAG 2 cut(s) 522, 876
StyD4I CCNGG 1 cut(s) 206
TaaI ACNGT 3 cut(s) 92, 224, 640
TaiI ACGT 3 cut(s) 248, 454, 806
TaqI TCGA 4 cut(s) 617, 679, 868, 891
TaqII GACCGA 1 cut(s) 120
TasI AATT 5 cut(s) 24, 260, 360, 736, 864
TatI WGTACW 1 cut(s) 628
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 5 cut(s) 127, 304, 364, 370, 435
Tru9I TTAA 5 cut(s) 127, 304, 364, 370, 435
TscAI CASTG 2 cut(s) 449, 755
TseFI GTSAC 1 cut(s) 116
TseI GCWGC 2 cut(s) 84, 201
Tsp45I GTSAC 1 cut(s) 116
TspDTI ATGAA 3 cut(s) 406, 667, 874
TspGWI ACGGA 2 cut(s) 116, 494
TspRI CASTG 2 cut(s) 449, 755
Van91I CCANNNNNTGG 1 cut(s) 831
VpaK11BI GGWCC 2 cut(s) 103, 585
VspI ATTAAT 1 cut(s) 370
XagI CCTNNNNNAGG 1 cut(s) 737
XapI RAATTY 4 cut(s) 24, 260, 360, 864
XceI RCATGY 1 cut(s) 799
XhoI CTCGAG 1 cut(s) 616
XspI CTAG 2 cut(s) 522, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.