Rmu_sc0002762.1_g000001

Transcription elongation factor that enhances transcription elongation by RNA polymerase II (RNAPII)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002762.1
OG0009494
Physical Location & Seq
Forward (+)
267 .. 8466
8200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 4920 bp
atgggcaaagctgtcatctccgacgacgaggatgaggtcgagctcgacgaggaggatagggaacccctcgacggggacacagtcgaccaggacggcggcggcggcgacgaagatgaggacgaagaagacgaagggccagatgaatatgagaaggatgattttatcgtagatgatgttgatgaagaagatgagcaggaggaagaagagcgggccgacagtgatgaggagaggcagaagaagaagaaaagaaagaaaaaagaggagtatgttcttgatgaagatgactacgagctcctggaggacaataatgtgattgccccgcgcagaaaggggaaatttaagcggctgaaaaaagctcagaggcatgctcagggggaccttggtggattatctgatgaggaggagtttcttggtactggtaaagggggaagaactgcagaggagaagcttaagttctcattatttggtggtgatgatgaaggccctccacttgaggacattgctgaagaggaggaaccagcagaagcggaagatgacggagaagatgaaatggcagactttatagtcgacgaagaatttgatgaggctggtgttcctgtcagacagaagaagcttaagaaaaagaaatccaggcaggcaccaggagtatcttcatctgctctgcaggaagcccatgacatatttggtgatgttgatgaattactgcagctgcgtaagcaaggtttggattctagtgaatggaaggaaaggaggcttgaagatgaatttgaaccaattgttctttcggaaaagtatatgacagagaaggatgaccagattcgggagattgatgtcccagaaagaattcaggtatatgaggaaagcactggttttcctccacttgatgaaaagagcatagatgatgagagtacctggatattcaatcaacttgcaagtggtacagttcctttttttggcaagacaggcctggggaattccataagcagggacgacatcattggattcttgaacttgcaccatgtgcagaaattggatgttccttttgttgcgatgtatcggaaagaggagtgccttagtatattgaaagatcctgaacacatggacatggatgatcaaaatgagaaagcatcaacactaaagtggcacaaggtactttggtccattcaggacttgcatagaaaatggttgctgcttcagaagaggaaaagtgctctgcaatcttattacgaaaagcggtttgatgaagaatctcgacgcatatatgatgagaccagactaactttaaatcagcaactttttgaatcaattatgaagtcactaaaggctgcagaatcagagagagaagttgatgatgtggacaccaagtttaacttgcatttccctgctggtgagataggtgtggatgagggccaatataagaggcctaagaggaagtccctttatagtatctgcagtaaggctggcttgtgggaggttgccagcaagtttggttacacctctgaacagtttggtttgcaattgtcactagagaaaatgagaatggatgagctggaggatgcgaaagagacgccagaggaattggcttcaaacttcacatgtgcaatgtttgagacacctcaggaagtgcttaaaggagccaggcatatggctgctgttgagataagctgtgagccatgtgtcaggaagtatgttaggagcaactacttggatatggttgagttgtccaccagcccaacaccagacggaaatactgcaattgaagcttttcatcagtttgcaggggtgaagtggttgcaaagaaagccattaaatagatttggggatgcacaatggctgcttatccagaaggctgaagaggagaagcttcttcaagttactattaagctaccggaagacagactaaacaagttgatgagcgactttaatgagtactatcttagtgatggtgttagtaaatctgcacaactgtggaatgagcagcggaagttgatattgcaggatgctcttttcaattttcttctaccctcaatggagaaagaagcaagatccttgttgactagtagagcaaaaaactggttattgatggaatacggaaaggttttgtggaataaagtctctgtgggaccatatcaacggaaagagaatgacattagttcagacgatgaagctgcacccagggtcatggcttgctgttggggccctgggaagccagcaaccacttttgttatgttagattcatcgggggaggtgctagatgtgctttacacgggatctctcaccttacgctcccagaacgttaacgatcagcaacggaaaaaaaatgaccaagaacgtgttttgaagttcatgactgatcaccaaccacatgtggctgttctaggagctgttaatttgtcttgtgttcgcctgaaagaagatatttacgagataatctttaagatggtggaggagaatcctagagatgttggacatgatatggatgggttaagcattgtctatggagatgaatctttggctcgcctgtatgaaaactctcgcatttcatctgatcagctacctgctcaatcaggcattgtgaagcgtgctgttgctctggggcgttaccttcaaaatccgttggcaatggttgccactctttgtgggccaggaagagaaatcttatcttggaaactcaatcctttggagaactttctcaccccagatgagaagtatgcaatgattgaacaagtcatggtagatgttacaaaccaagtaggcctcgacataaatttggctataagccacgaatggttatttgcacctttgcaattcatttctgggctcggacctagaaaagcagcatttttgcagcgatcgttggtaagatctggtgcaatatttactcggaaggactttttgacagcacatgggttaagtaaaaaggtgtttgttaatgcagttggtttcttgcgtgttcggcgtagtgggctggctgctagcagcagccaatttattgatctgttggatgatacaagaattcatccagaatcctatattcttgcacaagaattggccaaagatgtttttgaagttgatggtggcaatgatgatgaggatgctatggagatggctatagaacatgttagggatcggcctgcttatctaaaaaatcttgatgttgaagcgtatgctaaaagcaaagagcgcgagaataagatacaaacattttgtgacatcaggagagaattaatacagggttttcaggattggcgtaagaaatatgaagaactaagtcaagatgatgaattctatatgatttcaggtgaaactgaagatacccttgctgaaggaagaattgtgctggccacagttcgcagggtacaagctcaaagggcaatatgtgcacttgaatctggactgactggtatgcttatgaaagaagactattcagatgattcaagggatatttctgacttatctgagagactgaatgagggtgacattctcacgtgcaagatcaaatctattcaaaagaataggtatcaggtgttcctagtttgtagagagagtgaaatgagaaataatcgaaatcaaaatattcagcatctcgattcctattaccatgaagacaagagaagtctacagacagagcaagaaaaagctcgtaaggagaaagagcttgcgaaaaaacatttcaagccacggatgattgttcatcctcgcttccagaatattacggctgatgaagcaatgaagttcttatctgataaagatcctggggaaagtattattcgtcctagttcccgtgggccttcgtatttgaccttaactctgaaagtttacgatggagtttatgctcacaaagacgtagttgaaggtggaaaggaacacaaggatatcactagcttactccgtattggaaagactctgaaaattggagaggacacttttgaagatttagatgaggtaatggatcgttacgttgatcccctggtgtctcacttgaaggcaatgctaaattatcgcaaattccgacggggcacaaaagcagaggttgatgaactgttgaagattgaaaaattggagtttcctatgaggattgtttattgtttcgggatatcacatgaacatccagggactttcatactgacctatatacggagtacaaatccacaccatgagtatgttgggctgtatcctaaggggttcaagttcagaaaaaggatgtttgagagcattgatcggcttgtagcacattttcagaagtatattgatgagcctcaacatgagtccggaccatcaattagatcagttgctgctatggtgcccatgcgaagccctgcaactgggggttcttcgggtgcatccgcaggatggggtggttcaaacaatgagggtggttggagaggccagtcgtttgacagggatagatcttctacaccgagttcgcgaacaggtagaaatgattacagaaatggtggtggtcgagatggacatccaagtggcctacctagaccatatggggggcgcggtcgtggtagaggagcatataacgatagccgaggcaataatgagagacatgattcaggttttgatggtcctacatggggttcagataccaaggatggcaatgatgtcttgggcaactttccaggtgcaaaggttcaaaactcacctggcagggaagctttcccaggtggctggggtacaggtggaagtggaagtggtagtggaggaggcagcagctgggctggtggtggcggaagtggctggggtcaagaaagtggtggtggctcaggaggatggggtggggccgctgcaaatgatgctgctgctgataatgagccttcaggatggggttctgactcgaaaaagaagggttcgggtgatggtacttcaggatggggttctcagagtggaggtggcggtggtggttggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000018 GO:0000785 GO:0000988 GO:0000990 GO:0000991 GO:0002637 GO:0002682 GO:0002694 GO:0002697 GO:0002700 GO:0002703 GO:0002706 GO:0002712 GO:0002819 GO:0002822 GO:0002889 GO:0003006 GO:0003674 GO:0003682 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006325 GO:0006338 GO:0006342 GO:0006351 GO:0006354 GO:0006355 GO:0006357 GO:0006366 GO:0006368 GO:0006725 GO:0006807 GO:0006996 GO:0007275 GO:0008023 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009299 GO:0009506 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0010639 GO:0010793 GO:0016043 GO:0016070 GO:0016071 GO:0016458 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0030054 GO:0031056 GO:0031057 GO:0031060 GO:0031061 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0031399 GO:0031400 GO:0031491 GO:0031974 GO:0031981 GO:0032239 GO:0032268 GO:0032269 GO:0032386 GO:0032501 GO:0032502 GO:0032774 GO:0032784 GO:0032786 GO:0032879 GO:0032880 GO:0032968 GO:0032991 GO:0033043 GO:0033044 GO:0033157 GO:0034243 GO:0034641 GO:0034645 GO:0034654 GO:0034728 GO:0035327 GO:0040029 GO:0042393 GO:0042789 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043933 GO:0043954 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045191 GO:0045595 GO:0045814 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0046822 GO:0046825 GO:0046831 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048608 GO:0048731 GO:0048856 GO:0050684 GO:0050776 GO:0050789 GO:0050793 GO:0050794 GO:0050864 GO:0050865 GO:0051049 GO:0051052 GO:0051128 GO:0051129 GO:0051147 GO:0051171 GO:0051172 GO:0051173 GO:0051223 GO:0051239 GO:0051246 GO:0051248 GO:0051249 GO:0051252 GO:0051253 GO:0051254 GO:0051276 GO:0055044 GO:0060255 GO:0060341 GO:0061085 GO:0061086 GO:0061458 GO:0065007 GO:0070013 GO:0070201 GO:0070827 GO:0071704 GO:0071824 GO:0071840 GO:0080090 GO:0090087 GO:0090304 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1902275 GO:1902679 GO:1902680 GO:1903311 GO:1903506 GO:1903507 GO:1903508 GO:1903827 GO:1905268 GO:2000026 GO:2000112 GO:2000113 GO:2000197 GO:2001141 GO:2001251
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DLD HHH_7 HTH_44 SH2_2 SPT6_acidic YqgF

Protein Analysis

1639

Amino Acids

184.85

Weight (kDa)

5.0

Isoelectric Point (pI)

44.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT6_acidic PF14632 36 - 121 3.6e-16 Acidic N-terminal SPT6
HTH_44 PF14641 294 - 411 6.2e-06 Helix-turn-helix DNA-binding domain of SPT6
Tex_central_region PF22706 520 - 689 1.1e-06 Tex central region-like
YqgF PF14639 724 - 880 4.2e-17 Holliday-junction resolvase-like of SPT6
HHH_7 PF14635 884 - 985 1.1e-20 Helix-hairpin-helix motif
Spt6_S1 PF21710 1096 - 1184 1.4e-08 Spt6, S1/OB domain
SH2_2 PF14633 1200 - 1415 6e-69 SH2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 2566
AccB1I GGYRCC 2 cut(s) 637, 4303
AccBSI CCGCTC 1 cut(s) 208
AccI GTMKAC 3 cut(s) 84, 567, 3619
AccII CGCG 4 cut(s) 322, 3186, 4429, 4509
AccIII TCCGGA 1 cut(s) 4271
AclI AACGTT 1 cut(s) 2295
AclWI GGATC 7 cut(s) 1082, 2040, 2278, 3135, 3746, 3959, 3960
AcoI YGGCCR 2 cut(s) 3051, 3342
AcsI RAATTY 9 cut(s) 335, 575, 764, 843, 973, 2767, 3015, 3284, 4007
AcuI CTGAAG 7 cut(s) 525, 1178, 1872, 3330, 3345, 4812, 4860
AcvI CACGTG 1 cut(s) 3489
AcyI GRCGYC 1 cut(s) 1568
AdeI CACNNNGTG 1 cut(s) 1021
AfaI GTAC 9 cut(s) 415, 910, 940, 1152, 1931, 3360, 4144, 4687, 4873
AflII CTTAAG 2 cut(s) 449, 614
AflIII ACRYGT 4 cut(s) 1595, 2332, 2365, 3118
AhlI ACTAGT 1 cut(s) 2057
AjuI GAANNNNNNNTTGG 2 cut(s) 766, 798
AleI CACNNNNGTG 2 cut(s) 1138, 1966
Alw21I GWGCWC 4 cut(s) 45, 294, 1213, 3385
Alw26I GTCTC 7 cut(s) 1262, 1559, 1604, 2119, 3457, 3981, 4549
Alw44I GTGCAC 1 cut(s) 3381
AlwI GGATC 7 cut(s) 1082, 2040, 2278, 3135, 3746, 3959, 3960
AlwNI CAGNNNCTG 2 cut(s) 4295, 4725
Aor13HI TCCGGA 1 cut(s) 4271
ApaI GGGCCC 1 cut(s) 2201
ApaLI GTGCAC 1 cut(s) 3381
ApoI RAATTY 9 cut(s) 335, 575, 764, 843, 973, 2767, 3015, 3284, 4007
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AseI ATTAAT 1 cut(s) 3227
Asp700I GAANNNNTTC 2 cut(s) 843, 1764
AspLEI GCGC 3 cut(s) 324, 3186, 4509
AsuNHI GCTAGC 1 cut(s) 2975
AvaII GGWCC 6 cut(s) 376, 1158, 2123, 2825, 4274, 4577
AxyI CCTNAGG 2 cut(s) 1617, 4179
BaeGI GKGCMC 4 cut(s) 2201, 3385, 4022, 4308
BaeI ACNNNNGTAYC 2 cut(s) 1142, 1175
BalI TGGCCA 2 cut(s) 3053, 3344
BanI GGYRCC 2 cut(s) 637, 4303
BanII GRGCYC 4 cut(s) 45, 294, 2201, 2823
BbrPI CACGTG 1 cut(s) 3489
BbsI GAAGAC 4 cut(s) 132, 1899, 3426, 3612
Bbv12I GWGCWC 4 cut(s) 45, 294, 1213, 3385
BceAI ACGGC 2 cut(s) 109, 3732
BcgI CGANNNNNNTGC 2 cut(s) 2150, 2184
BciVI GTATCC 1 cut(s) 4185
BclI TGATCA 3 cut(s) 1111, 2353, 2548
BcoDI GTCTC 7 cut(s) 1262, 1559, 1604, 2119, 3457, 3981, 4549
BcuI ACTAGT 1 cut(s) 2057
BfmI CTRYAG 7 cut(s) 435, 662, 704, 1326, 1450, 3111, 3620
BfrI CTTAAG 2 cut(s) 449, 614
BfuAI ACCTGC 1 cut(s) 2566
BfuI GTATCC 1 cut(s) 4185
BglII AGATCT 2 cut(s) 2864, 4409
BmcAI AGTACT 1 cut(s) 1931
Bme18I GGWCC 6 cut(s) 376, 1158, 2123, 2825, 4274, 4577
BmrI ACTGGG 1 cut(s) 4335
BmsI GCATC 8 cut(s) 1136, 1546, 1813, 1990, 3085, 3592, 4352, 4795
BmtI GCTAGC 1 cut(s) 2979
BmuI ACTGGG 1 cut(s) 4335
BpiI GAAGAC 4 cut(s) 132, 1899, 3426, 3612
BplI GAGNNNNNCTC 2 cut(s) 352, 384
BpmI CTGGAG 2 cut(s) 317, 1571
Bpu10I CCTNAGC 2 cut(s) 369, 4774
BpuEI CTTGAG 1 cut(s) 512
BsaAI YACGTR 1 cut(s) 3489
BsaBI GATNNNNATC 2 cut(s) 3750, 4473
BsaHI GRCGYC 1 cut(s) 1568
BsaI GGTCTC 1 cut(s) 1262
BsaWI WCCGGW 2 cut(s) 1888, 4271
Bse1I ACTGG 6 cut(s) 421, 871, 2078, 3406, 4330, 4390
Bse21I CCTNAGG 2 cut(s) 1617, 4179
Bse3DI GCAATG 8 cut(s) 498, 1608, 2628, 2721, 3088, 3735, 3996, 4615
Bse8I GATNNNNATC 2 cut(s) 3750, 4473
BseAI TCCGGA 1 cut(s) 4271
BseJI GATNNNNATC 2 cut(s) 3750, 4473
BseMI GCAATG 8 cut(s) 498, 1608, 2628, 2721, 3088, 3735, 3996, 4615
BseMII CTCAG 6 cut(s) 371, 383, 1631, 3450, 4788, 4904
BseNI ACTGG 6 cut(s) 421, 871, 2078, 3406, 4330, 4390
BseSI GKGCMC 4 cut(s) 2201, 3385, 4022, 4308
BseYI CCCAGC 3 cut(s) 4680, 4725, 4749
BsgI GTGCAG 3 cut(s) 1043, 1944, 2154
Bsh1236I CGCG 4 cut(s) 322, 3186, 4429, 4509
Bsh1285I CGRYCG 2 cut(s) 2855, 4513
BshNI GGYRCC 2 cut(s) 637, 4303
BsiEI CGRYCG 2 cut(s) 2855, 4513
BsiHKAI GWGCWC 4 cut(s) 45, 294, 1213, 3385
BsiSI CCGG 2 cut(s) 1889, 4272
BslFI GGGAC 7 cut(s) 89, 389, 818, 1001, 1420, 2136, 4129
BsmAI GTCTC 7 cut(s) 1262, 1559, 1604, 2119, 3457, 3981, 4549
BsmBI CGTCTC 1 cut(s) 1559
BsmFI GGGAC 7 cut(s) 89, 389, 818, 1001, 1420, 2136, 4129
Bso31I GGTCTC 1 cut(s) 1262
Bsp120I GGGCCC 1 cut(s) 2197
Bsp1286I GDGCHC 8 cut(s) 45, 294, 1213, 2201, 2823, 3385, 4022, 4308
Bsp13I TCCGGA 1 cut(s) 4271
Bsp68I TCGCGA 1 cut(s) 4429
BspCNI CTCAG 6 cut(s) 370, 382, 1630, 3451, 4787, 4903
BspEI TCCGGA 1 cut(s) 4271
BspFNI CGCG 4 cut(s) 322, 3186, 4429, 4509
BspHI TCATGA 1 cut(s) 2346
BspMAI CTGCAG 5 cut(s) 439, 666, 708, 1330, 1454
BspMI ACCTGC 1 cut(s) 2566
BspOI GCTAGC 1 cut(s) 2979
BspPI GGATC 7 cut(s) 1082, 2040, 2278, 3135, 3746, 3959, 3960
BspQI GCTCTTC 1 cut(s) 198
BspT107I GGYRCC 2 cut(s) 637, 4303
BspTI CTTAAG 2 cut(s) 449, 614
BspTNI GGTCTC 1 cut(s) 1262
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 8 cut(s) 498, 1608, 2628, 2721, 3088, 3735, 3996, 4615
BsrI ACTGG 6 cut(s) 421, 871, 2078, 3406, 4330, 4390
BssNI GRCGYC 1 cut(s) 1568
BssT1I CCWWGG 2 cut(s) 379, 4599
Bst4CI ACNGT 7 cut(s) 82, 218, 943, 1506, 1968, 3349, 4044
Bst6I CTCTTC 5 cut(s) 198, 501, 1193, 1848, 2644
BstACI GRCGYC 1 cut(s) 1568
BstAFI CTTAAG 2 cut(s) 449, 614
BstAPI GCANNNNNTGC 4 cut(s) 1021, 2627, 3380, 4805
BstBAI YACGTR 1 cut(s) 3489
BstDSI CCRYGG 2 cut(s) 3680, 3784
BstFNI CGCG 4 cut(s) 322, 3186, 4429, 4509
BstHHI GCGC 3 cut(s) 324, 3186, 4509
BstMAI GTCTC 7 cut(s) 1262, 1559, 1604, 2119, 3457, 3981, 4549
BstMCI CGRYCG 2 cut(s) 2855, 4513
BstNSI RCATGY 4 cut(s) 368, 1599, 2369, 3122
BstSFI CTRYAG 7 cut(s) 435, 662, 704, 1326, 1450, 3111, 3620
BstSLI GKGCMC 4 cut(s) 2201, 3385, 4022, 4308
BstUI CGCG 4 cut(s) 322, 3186, 4429, 4509
BstV2I GAAGAC 4 cut(s) 132, 1899, 3426, 3612
BstX2I RGATCY 6 cut(s) 1087, 2045, 2270, 2864, 3751, 4409
BstXI CCANNNNNNTGG 3 cut(s) 1645, 2182, 4680
BstYI RGATCY 6 cut(s) 1087, 2045, 2270, 2864, 3751, 4409
Bsu36I CCTNAGG 2 cut(s) 1617, 4179
BsuI GTATCC 1 cut(s) 4185
BtgI CCRYGG 2 cut(s) 3680, 3784
BtgZI GCGATG 1 cut(s) 1064
BtsIMutI CAGTG 2 cut(s) 223, 864
BtuMI TCGCGA 1 cut(s) 4429
BveI ACCTGC 1 cut(s) 2566
CaiI CAGNNNCTG 2 cut(s) 4295, 4725
CciI TCATGA 1 cut(s) 2346
CfoI GCGC 3 cut(s) 324, 3186, 4509
CseI GACGC 2 cut(s) 1263, 1576
Csp6I GTAC 9 cut(s) 414, 909, 939, 1151, 1930, 3359, 4143, 4686, 4872
CspCI CAANNNNNGTGG 2 cut(s) 4357, 4392
CviQI GTAC 9 cut(s) 414, 909, 939, 1151, 1930, 3359, 4143, 4686, 4872
DraI TTTAAA 1 cut(s) 1284
DraIII CACNNNGTG 1 cut(s) 1021
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
EaeI YGGCCR 2 cut(s) 3051, 3342
Eam1104I CTCTTC 5 cut(s) 198, 501, 1193, 1848, 2644
EarI CTCTTC 5 cut(s) 198, 501, 1193, 1848, 2644
EciI GGCGGA 1 cut(s) 4755
Ecl136II GAGCTC 2 cut(s) 43, 292
Eco130I CCWWGG 2 cut(s) 379, 4599
Eco147I AGGCCT 3 cut(s) 966, 1423, 2757
Eco24I GRGCYC 4 cut(s) 45, 294, 2201, 2823
Eco31I GGTCTC 1 cut(s) 1262
Eco32I GATATC 2 cut(s) 3877, 4098
Eco47I GGWCC 6 cut(s) 376, 1158, 2123, 2825, 4274, 4577
Eco53kI GAGCTC 2 cut(s) 43, 292
Eco57I CTGAAG 7 cut(s) 525, 1178, 1872, 3330, 3345, 4812, 4860
Eco72I CACGTG 1 cut(s) 3489
Eco81I CCTNAGG 2 cut(s) 1617, 4179
EcoICRI GAGCTC 2 cut(s) 43, 292
EcoO109I RGGNCCY 4 cut(s) 376, 482, 2197, 2198
EcoRI GAATTC 4 cut(s) 843, 973, 3015, 3284
EcoRV GATATC 2 cut(s) 3877, 4098
EcoT14I CCWWGG 2 cut(s) 379, 4599
EcoT38I GRGCYC 4 cut(s) 45, 294, 2201, 2823
ErhI CCWWGG 2 cut(s) 379, 4599
Esp3I CGTCTC 1 cut(s) 1559
FalI AAGNNNNNCTT 6 cut(s) 1355, 1387, 1448, 1480, 3303, 3335
FaqI GGGAC 7 cut(s) 89, 389, 818, 1001, 1420, 2136, 4129
FauI CCCGC 2 cut(s) 201, 327
FauNDI CATATG 2 cut(s) 1644, 4498
FbaI TGATCA 3 cut(s) 1111, 2353, 2548
FblI GTMKAC 3 cut(s) 84, 567, 3619
FriOI GRGCYC 4 cut(s) 45, 294, 2201, 2823
GlaI GCGC 3 cut(s) 323, 3185, 4508
GsaI CCCAGC 3 cut(s) 4684, 4729, 4753
GsuI CTGGAG 2 cut(s) 317, 1571
HapII CCGG 2 cut(s) 1889, 4272
HgaI GACGC 2 cut(s) 1263, 1576
HhaI GCGC 3 cut(s) 324, 3186, 4509
Hin1I GRCGYC 1 cut(s) 1568
Hin6I GCGC 3 cut(s) 322, 3184, 4507
HinP1I GCGC 3 cut(s) 322, 3184, 4507
HincII GTYRAC 4 cut(s) 85, 568, 2055, 2299
HindII GTYRAC 4 cut(s) 85, 568, 2055, 2299
HindIII AAGCTT 5 cut(s) 446, 611, 1761, 1862, 4665
HpaI GTTAAC 1 cut(s) 2299
HpaII CCGG 2 cut(s) 1889, 4272
Hpy166II GTNNAC 9 cut(s) 85, 568, 1357, 1725, 2055, 2299, 3383, 3620, 3820
Hpy8I GTNNAC 9 cut(s) 85, 568, 1357, 1725, 2055, 2299, 3383, 3620, 3820
Hpy99I CGWCG 8 cut(s) 26, 29, 50, 74, 110, 572, 1257, 4017
HpyCH4III ACNGT 7 cut(s) 82, 218, 943, 1506, 1968, 3349, 4044
HpyCH4IV ACGT 5 cut(s) 2295, 2332, 3488, 3846, 3960
HpySE526I ACGT 5 cut(s) 2295, 2332, 3488, 3846, 3960
Hsp92I GRCGYC 1 cut(s) 1568
HspAI GCGC 3 cut(s) 322, 3184, 4507
Kpn2I TCCGGA 1 cut(s) 4271
Ksp22I TGATCA 3 cut(s) 1111, 2353, 2548
KspAI GTTAAC 1 cut(s) 2299
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 6 cut(s) 297, 1634, 1695, 2291, 2381, 4523
LweI GCATC 8 cut(s) 1136, 1546, 1813, 1990, 3085, 3592, 4352, 4795
MaeII ACGT 5 cut(s) 2295, 2332, 3488, 3846, 3960
MaeIII GTNAC 9 cut(s) 1314, 1490, 1521, 1873, 2600, 2740, 3209, 3476, 3956
MbiI CCGCTC 1 cut(s) 208
MfeI CAATTG 3 cut(s) 774, 1517, 1755
MflI RGATCY 6 cut(s) 1087, 2045, 2270, 2864, 3751, 4409
MhlI GDGCHC 8 cut(s) 45, 294, 1213, 2201, 2823, 3385, 4022, 4308
MlsI TGGCCA 2 cut(s) 3053, 3344
MluNI TGGCCA 2 cut(s) 3053, 3344
MlyI GAGTC 3 cut(s) 3898, 4277, 4838
MmeI TCCRAC 5 cut(s) 45, 2446, 2982, 4036, 4361
Mox20I TGGCCA 2 cut(s) 3053, 3344
MroI TCCGGA 1 cut(s) 4271
MroXI GAANNNNTTC 2 cut(s) 843, 1764
MscI TGGCCA 2 cut(s) 3053, 3344
MslI CAYNNNNRTG 5 cut(s) 1103, 1138, 1966, 4161, 4479
Msp20I TGGCCA 2 cut(s) 3053, 3344
MspA1I CMGCKG 4 cut(s) 709, 1981, 4725, 4796
MspCI CTTAAG 2 cut(s) 449, 614
MspI CCGG 2 cut(s) 1889, 4272
MunI CAATTG 3 cut(s) 774, 1517, 1755
MvnI CGCG 4 cut(s) 322, 3186, 4429, 4509
NdeI CATATG 2 cut(s) 1644, 4498
NheI GCTAGC 1 cut(s) 2975
NmeAIII GCCGAG 1 cut(s) 4565
NmuCI GTSAC 4 cut(s) 1314, 1521, 3209, 3476
NruI TCGCGA 1 cut(s) 4429
NspI RCATGY 4 cut(s) 368, 1599, 2369, 3122
OliI CACNNNNGTG 2 cut(s) 1138, 1966
PaeI GCATGC 1 cut(s) 368
PagI TCATGA 1 cut(s) 2346
PasI CCCWGGG 2 cut(s) 2175, 2201
PceI AGGCCT 3 cut(s) 966, 1423, 2757
PciI ACATGT 3 cut(s) 1595, 2365, 3118
PciSI GCTCTTC 1 cut(s) 198
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 126, 4009
PdmI GAANNNNTTC 2 cut(s) 843, 1764
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 294
Ple19I CGATCG 1 cut(s) 2855
PleI GAGTC 3 cut(s) 3898, 4276, 4838
PmaCI CACGTG 1 cut(s) 3489
PmlI CACGTG 1 cut(s) 3489
PpsI GAGTC 3 cut(s) 3898, 4276, 4838
Ppu21I YACGTR 1 cut(s) 3489
PpuMI RGGWCCY 1 cut(s) 376
PscI ACATGT 3 cut(s) 1595, 2365, 3118
PshBI ATTAAT 1 cut(s) 3227
Psp124BI GAGCTC 2 cut(s) 45, 294
Psp1406I AACGTT 1 cut(s) 2295
Psp5II RGGWCCY 1 cut(s) 376
PspCI CACGTG 1 cut(s) 3489
PspFI CCCAGC 3 cut(s) 4680, 4725, 4749
PspOMI GGGCCC 1 cut(s) 2197
PspPPI RGGWCCY 1 cut(s) 376
PsrI GAACNNNNNNTAC 2 cut(s) 3512, 3544
PstI CTGCAG 5 cut(s) 439, 666, 708, 1330, 1454
PstNI CAGNNNCTG 2 cut(s) 4295, 4725
PsuI RGATCY 6 cut(s) 1087, 2045, 2270, 2864, 3751, 4409
PsyI GACNNNGTC 1 cut(s) 80
PvuI CGATCG 1 cut(s) 2855
PvuII CAGCTG 2 cut(s) 709, 4725
RruI TCGCGA 1 cut(s) 4429
RsaI GTAC 9 cut(s) 415, 910, 940, 1152, 1931, 3360, 4144, 4687, 4873
RsaNI GTAC 9 cut(s) 414, 909, 939, 1151, 1930, 3359, 4143, 4686, 4872
RseI CAYNNNNRTG 5 cut(s) 1103, 1138, 1966, 4161, 4479
SacI GAGCTC 2 cut(s) 45, 294
SalI GTCGAC 2 cut(s) 83, 566
SapI GCTCTTC 1 cut(s) 198
ScaI AGTACT 1 cut(s) 1931
SchI GAGTC 3 cut(s) 3898, 4277, 4838
SduI GDGCHC 8 cut(s) 45, 294, 1213, 2201, 2823, 3385, 4022, 4308
SfaNI GCATC 8 cut(s) 1136, 1546, 1813, 1990, 3085, 3592, 4352, 4795
SfcI CTRYAG 7 cut(s) 435, 662, 704, 1326, 1450, 3111, 3620
SinI GGWCC 6 cut(s) 376, 1158, 2123, 2825, 4274, 4577
SmiMI CAYNNNNRTG 5 cut(s) 1103, 1138, 1966, 4161, 4479
SmlI CTYRAG 3 cut(s) 449, 491, 614
SmoI CTYRAG 3 cut(s) 449, 491, 614
SpeI ACTAGT 1 cut(s) 2057
SphI GCATGC 1 cut(s) 368
SseBI AGGCCT 3 cut(s) 966, 1423, 2757
SspI AATATT 3 cut(s) 2877, 3577, 3712
SstI GAGCTC 2 cut(s) 45, 294
StuI AGGCCT 3 cut(s) 966, 1423, 2757
StyI CCWWGG 2 cut(s) 379, 4599
TaaI ACNGT 7 cut(s) 82, 218, 943, 1506, 1968, 3349, 4044
TaiI ACGT 5 cut(s) 2298, 2335, 3491, 3849, 3963
TatI WGTACW 2 cut(s) 1929, 4142
TauI GCSGC 5 cut(s) 99, 102, 105, 346, 4796
TscAI CASTG 2 cut(s) 223, 871
TseFI GTSAC 4 cut(s) 1314, 1521, 3209, 3476
Tsp45I GTSAC 4 cut(s) 1314, 1521, 3209, 3476
TspGWI ACGGA 9 cut(s) 552, 1758, 2106, 2149, 2326, 2604, 3697, 3881, 4153
TspRI CASTG 2 cut(s) 223, 871
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 2 cut(s) 449, 614
VneI GTGCAC 1 cut(s) 3381
VpaK11BI GGWCC 6 cut(s) 376, 1158, 2123, 2825, 4274, 4577
VspI ATTAAT 1 cut(s) 3227
XapI RAATTY 9 cut(s) 335, 575, 764, 843, 973, 2767, 3015, 3284, 4007
XceI RCATGY 4 cut(s) 368, 1599, 2369, 3122
XcmI CCANNNNNNNNNTGG 2 cut(s) 680, 4163
XmiI GTMKAC 3 cut(s) 84, 567, 3619
XmnI GAANNNNTTC 2 cut(s) 843, 1764
ZrmI AGTACT 1 cut(s) 1931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.