Rmu_sc0003576.1_g000010

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003576.1
OG0017455
Physical Location & Seq
Forward (+)
38889 .. 41604
2716 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1203 bp
atggatttgcttcaggctctcttttccccatcagaggttgaggtcattgggcggatccctcttagtgtgcgtgagtcggaggatagacttatttggcactatgataggaagggctgctacactgttcgtaatgggtaccatgtagcgcgtacagctgctgaacttgattctagggcatctagctctaatggctcatttggagtccatagatcatattggaagggtatttggcatgcttgcattccacctaaggtacgttcttttatttggcgcgctgtaaaaggtattttgcccactaaggtctctcttgctaggcgtgttgccctccccaatatgaaatgtgcgttttgtgctaatgcaatagaggatggtattcacttatttagagattgtgatgtcattggagacctttggctacttagctcagtgggtttgaatgtgaaacatgttcctggaagaagtttggaagaatgggtgctcaatgttttggatgttttgcatggtggtctacgtgaggctttcttcatgtacctttggctgcccccacctgggcgtctcaaggtgaatgtggatggtagttacaggccaaaatttggagatggtggaattggagttgttgtcagagacgagaggcttgttgagccggagctcttttggctattcaccaagattgggacactttcgatcttgagaattatacatattccccgtgaagcaaatggtgtagcccatagattggctcaccttgctagtcttaattatctggatgatatttggttagatgaggctcctgtgattatccaggatgttctctacgaggatttatgtactggtgctcgagataggcgtcggctgggcagcgtggtgcgtctgggcgaccggtatcaaggaagcagattgcaatggttcgatgagaggattgttgaccgcgtagcggagaggcaacaacatctgatgttgttgcactgctggaacggctggccaccagagttgtggtcgacggcagtgccgccggagggtatgacggcggcggaggtaaccaagcaacatgaggctcctgttctgctggcctgggattgggcctgggtctctggacctgggctcaatcttgggctgataggcctggttgattttgtttctgctaggcaagactggacttgggcttgggctcgggtcaaggcaattggggccctaatgtgttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

400

Amino Acids

45.36

Weight (kDa)

7.67

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 70 - 138 8.3e-14 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 135
AccB1I GGYRCC 1 cut(s) 135
AccB7I CCANNNNNTGG 2 cut(s) 593, 736
AccI GTMKAC 2 cut(s) 508, 998
AccII CGCG 3 cut(s) 148, 273, 930
AciI CCGC 6 cut(s) 52, 928, 935, 1010, 1028, 1031
AclWI GGATC 2 cut(s) 49, 62
AcoI YGGCCR 1 cut(s) 980
AcsI RAATTY 1 cut(s) 590
AcyI GRCGYC 2 cut(s) 553, 847
AfaI GTAC 5 cut(s) 137, 151, 255, 530, 829
AfiI CCNNNNNNNGG 9 cut(s) 34, 548, 549, 593, 672, 736, 934, 1016, 1077
AflIII ACRYGT 1 cut(s) 445
AgeI ACCGGT 1 cut(s) 879
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
AluBI AGCT 4 cut(s) 155, 183, 423, 649
AluI AGCT 4 cut(s) 155, 183, 423, 649
Alw21I GWGCWC 3 cut(s) 480, 651, 838
Alw26I GTCTC 5 cut(s) 307, 399, 560, 618, 1093
AlwI GGATC 2 cut(s) 49, 62
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 2 cut(s) 837, 1170
AoxI GGCC 6 cut(s) 584, 980, 1068, 1080, 1120, 1188
ApaI GGGCCC 1 cut(s) 1192
ApeKI GCWGC 4 cut(s) 114, 155, 538, 858
ApoI RAATTY 1 cut(s) 590
AsiGI ACCGGT 1 cut(s) 879
Asp718I GGTACC 1 cut(s) 135
AspLEI GCGC 3 cut(s) 148, 273, 275
AspS9I GGNCC 4 cut(s) 1080, 1094, 1188, 1189
AsuHPI GGTGA 3 cut(s) 574, 655, 734
AvaI CYCGRG 2 cut(s) 837, 1170
AvaII GGWCC 1 cut(s) 1094
AxyI CCTNAGG 1 cut(s) 249
BaeGI GKGCMC 1 cut(s) 1192
BalI TGGCCA 1 cut(s) 982
BamHI GGATCC 1 cut(s) 54
BanI GGYRCC 1 cut(s) 135
BanII GRGCYC 4 cut(s) 651, 1104, 1171, 1192
BarI GAAGNNNNNNTAC 2 cut(s) 101, 133
Bbv12I GWGCWC 3 cut(s) 480, 651, 838
BbvI GCAGC 4 cut(s) 101, 142, 525, 870
BccI CCATC 4 cut(s) 37, 362, 566, 593
BceAI ACGGC 3 cut(s) 991, 1017, 1041
BcgI CGANNNNNNTGC 2 cut(s) 988, 1022
BciT130I CCWGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
BcoDI GTCTC 5 cut(s) 307, 399, 560, 618, 1093
BfaI CTAG 5 cut(s) 171, 180, 312, 750, 1143
BisI GCNGC 6 cut(s) 115, 156, 539, 859, 1010, 1029
BlsI GCNGC 6 cut(s) 116, 157, 540, 860, 1011, 1030
Bme1390I CCNGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
Bme18I GGWCC 1 cut(s) 1094
BmeT110I CYCGRG 2 cut(s) 837, 1170
BmgT120I GGNCC 4 cut(s) 1080, 1094, 1188, 1189
BmiI GGNNCC 6 cut(s) 56, 137, 789, 1056, 1189, 1190
BmrFI CCNGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
BmsI GCATC 1 cut(s) 185
BpuEI CTTGAG 2 cut(s) 542, 709
BsaAI YACGTR 1 cut(s) 512
BsaHI GRCGYC 2 cut(s) 553, 847
BsaI GGTCTC 3 cut(s) 307, 399, 1093
BsaJI CCNNGG 4 cut(s) 548, 1071, 1083, 1097
BsaWI WCCGGW 1 cut(s) 879
BsaXI ACNNNNNCTCC 2 cut(s) 603, 633
Bsc4I CCNNNNNNNGG 9 cut(s) 34, 548, 549, 593, 672, 736, 934, 1016, 1077
Bse118I RCCGGY 1 cut(s) 879
Bse1I ACTGG 2 cut(s) 835, 1157
Bse21I CCTNAGG 1 cut(s) 249
Bse3DI GCAATG 1 cut(s) 908
BseBI CCWGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
BseDI CCNNGG 4 cut(s) 548, 1071, 1083, 1097
BseGI GGATG 5 cut(s) 373, 496, 577, 772, 811
BseLI CCNNNNNNNGG 9 cut(s) 34, 548, 549, 593, 672, 736, 934, 1016, 1077
BseMI GCAATG 1 cut(s) 908
BseMII CTCAG 1 cut(s) 438
BseNI ACTGG 2 cut(s) 835, 1157
BsePI GCGCGC 1 cut(s) 271
BseSI GKGCMC 1 cut(s) 1192
BseXI GCAGC 4 cut(s) 101, 142, 525, 870
BseYI CCCAGC 1 cut(s) 853
Bsh1236I CGCG 3 cut(s) 148, 273, 930
Bsh1285I CGRYCG 1 cut(s) 880
BshFI GGCC 6 cut(s) 586, 982, 1070, 1082, 1122, 1190
BshNI GGYRCC 1 cut(s) 135
BshTI ACCGGT 1 cut(s) 879
BsiEI CGRYCG 1 cut(s) 880
BsiHKAI GWGCWC 3 cut(s) 480, 651, 838
BsiHKCI CYCGRG 2 cut(s) 837, 1170
BsiSI CCGG 3 cut(s) 644, 880, 1013
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 9 cut(s) 34, 548, 549, 593, 672, 736, 934, 1016, 1077
BsmAI GTCTC 5 cut(s) 307, 399, 560, 618, 1093
BsmBI CGTCTC 2 cut(s) 560, 618
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 6 cut(s) 586, 982, 1070, 1082, 1122, 1190
Bso31I GGTCTC 3 cut(s) 307, 399, 1093
BsoBI CYCGRG 2 cut(s) 837, 1170
Bsp120I GGGCCC 1 cut(s) 1188
Bsp1286I GDGCHC 6 cut(s) 480, 651, 838, 1104, 1171, 1192
Bsp143I GATC 3 cut(s) 54, 209, 684
BspACI CCGC 6 cut(s) 52, 928, 935, 1010, 1028, 1031
BspANI GGCC 6 cut(s) 586, 982, 1070, 1082, 1122, 1190
BspCNI CTCAG 1 cut(s) 437
BspFNI CGCG 3 cut(s) 148, 273, 930
BspLI GGNNCC 6 cut(s) 56, 137, 789, 1056, 1189, 1190
BspPI GGATC 2 cut(s) 49, 62
BspT107I GGYRCC 1 cut(s) 135
BspTNI GGTCTC 3 cut(s) 307, 399, 1093
BsrDI GCAATG 1 cut(s) 908
BsrFI RCCGGY 1 cut(s) 879
BsrI ACTGG 2 cut(s) 835, 1157
BssAI RCCGGY 1 cut(s) 879
BssECI CCNNGG 4 cut(s) 548, 1071, 1083, 1097
BssHII GCGCGC 1 cut(s) 271
BssMI GATC 3 cut(s) 54, 209, 684
BssNI GRCGYC 2 cut(s) 553, 847
Bst2UI CCWGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
Bst4CI ACNGT 1 cut(s) 124
BstACI GRCGYC 2 cut(s) 553, 847
BstBAI YACGTR 1 cut(s) 512
BstC8I GCNNGC 5 cut(s) 234, 238, 273, 980, 1068
BstDEI CTNAG 5 cut(s) 62, 249, 297, 419, 424
BstEII GGTNACC 1 cut(s) 1036
BstF5I GGATG 5 cut(s) 373, 496, 577, 772, 811
BstFNI CGCG 3 cut(s) 148, 273, 930
BstHHI GCGC 3 cut(s) 148, 273, 275
BstKTI GATC 3 cut(s) 57, 212, 687
BstMAI GTCTC 5 cut(s) 307, 399, 560, 618, 1093
BstMBI GATC 3 cut(s) 54, 209, 684
BstMCI CGRYCG 1 cut(s) 880
BstMWI GCNNNNNNNGC 8 cut(s) 152, 189, 350, 640, 655, 746, 975, 1187
BstNI CCWGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
BstNSI RCATGY 2 cut(s) 236, 449
BstPI GGTNACC 1 cut(s) 1036
BstSCI CCNGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
BstSLI GKGCMC 1 cut(s) 1192
BstUI CGCG 3 cut(s) 148, 273, 930
BstV1I GCAGC 4 cut(s) 101, 142, 525, 870
BstX2I RGATCY 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 1 cut(s) 54
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 6 cut(s) 586, 982, 1070, 1082, 1122, 1190
BtsCI GGATG 5 cut(s) 373, 496, 577, 772, 811
BtsI GCAGTG 2 cut(s) 964, 1011
BtsIMutI CAGTG 4 cut(s) 120, 432, 964, 1011
Cac8I GCNNGC 5 cut(s) 234, 238, 273, 980, 1068
CaiI CAGNNNCTG 1 cut(s) 158
CfoI GCGC 3 cut(s) 148, 273, 275
Cfr10I RCCGGY 1 cut(s) 879
Cfr13I GGNCC 4 cut(s) 1080, 1094, 1188, 1189
CseI GACGC 3 cut(s) 542, 836, 857
Csp6I GTAC 5 cut(s) 136, 150, 254, 529, 828
CspAI ACCGGT 1 cut(s) 879
CspCI CAANNNNNGTGG 2 cut(s) 972, 1007
CviAII CATG 6 cut(s) 140, 233, 446, 500, 526, 1049
CviQI GTAC 5 cut(s) 136, 150, 254, 529, 828
DdeI CTNAG 5 cut(s) 62, 249, 297, 419, 424
DpnI GATC 3 cut(s) 56, 211, 686
DpnII GATC 3 cut(s) 54, 209, 684
EaeI YGGCCR 1 cut(s) 980
EciI GGCGGA 2 cut(s) 67, 1046
Ecl136II GAGCTC 1 cut(s) 649
Eco147I AGGCCT 1 cut(s) 1122
Eco24I GRGCYC 4 cut(s) 651, 1104, 1171, 1192
Eco31I GGTCTC 3 cut(s) 307, 399, 1093
Eco47I GGWCC 1 cut(s) 1094
Eco53kI GAGCTC 1 cut(s) 649
Eco81I CCTNAGG 1 cut(s) 249
Eco88I CYCGRG 2 cut(s) 837, 1170
Eco91I GGTNACC 1 cut(s) 1036
EcoICRI GAGCTC 1 cut(s) 649
EcoO109I RGGNCCY 2 cut(s) 1188, 1189
EcoO65I GGTNACC 1 cut(s) 1036
EcoRII CCWGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
EcoT38I GRGCYC 4 cut(s) 651, 1104, 1171, 1192
Esp3I CGTCTC 2 cut(s) 560, 618
FaeI CATG 6 cut(s) 143, 236, 449, 503, 529, 1052
FaqI GGGAC 1 cut(s) 688
FatI CATG 6 cut(s) 139, 232, 445, 499, 525, 1048
FblI GTMKAC 2 cut(s) 508, 998
Fnu4HI GCNGC 6 cut(s) 115, 156, 539, 859, 1010, 1029
FokI GGATG 5 cut(s) 380, 503, 584, 779, 818
FriOI GRGCYC 4 cut(s) 651, 1104, 1171, 1192
Fsp4HI GCNGC 6 cut(s) 115, 156, 539, 859, 1010, 1029
FspBI CTAG 5 cut(s) 171, 180, 312, 750, 1143
GlaI GCGC 3 cut(s) 147, 272, 274
GluI GCNGC 6 cut(s) 115, 156, 539, 859, 1010, 1029
GsaI CCCAGC 1 cut(s) 857
HaeIII GGCC 6 cut(s) 586, 982, 1070, 1082, 1122, 1190
HapII CCGG 3 cut(s) 644, 880, 1013
HgaI GACGC 3 cut(s) 542, 836, 857
HhaI GCGC 3 cut(s) 148, 273, 275
Hin1I GRCGYC 2 cut(s) 553, 847
Hin1II CATG 6 cut(s) 143, 236, 449, 503, 529, 1052
Hin6I GCGC 3 cut(s) 146, 271, 273
HinP1I GCGC 3 cut(s) 146, 271, 273
HincII GTYRAC 2 cut(s) 925, 999
HindII GTYRAC 2 cut(s) 925, 999
HinfI GANTC 3 cut(s) 74, 167, 201
HpaII CCGG 3 cut(s) 644, 880, 1013
HphI GGTGA 3 cut(s) 574, 655, 734
Hpy166II GTNNAC 3 cut(s) 509, 925, 999
Hpy188I TCNGA 4 cut(s) 34, 79, 623, 954
Hpy188III TCNNGA 4 cut(s) 688, 764, 839, 1092
Hpy8I GTNNAC 3 cut(s) 509, 925, 999
Hpy99I CGWCG 2 cut(s) 852, 1003
HpyAV CCTTC 2 cut(s) 103, 214
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 2 cut(s) 256, 511
HpyCH4V TGCA 5 cut(s) 240, 359, 499, 901, 964
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 189, 350, 640, 655, 746, 975, 1187
HpyF3I CTNAG 5 cut(s) 62, 249, 297, 419, 424
HpySE526I ACGT 2 cut(s) 256, 511
Hsp92I GRCGYC 2 cut(s) 553, 847
Hsp92II CATG 6 cut(s) 143, 236, 449, 503, 529, 1052
HspAI GCGC 3 cut(s) 146, 271, 273
KpnI GGTACC 1 cut(s) 139
Kzo9I GATC 3 cut(s) 54, 209, 684
LmnI GCTCC 3 cut(s) 646, 793, 1060
Lsp1109I GCAGC 4 cut(s) 101, 142, 525, 870
LweI GCATC 1 cut(s) 185
MaeI CTAG 5 cut(s) 171, 180, 312, 750, 1143
MaeII ACGT 2 cut(s) 256, 511
MaeIII GTNAC 2 cut(s) 578, 1036
MalI GATC 3 cut(s) 56, 211, 686
MboI GATC 3 cut(s) 54, 209, 684
MboII GAAGA 3 cut(s) 468, 479, 514
MfeI CAATTG 1 cut(s) 1182
MflI RGATCY 1 cut(s) 54
MhlI GDGCHC 6 cut(s) 480, 651, 838, 1104, 1171, 1192
MlsI TGGCCA 1 cut(s) 982
MluCI AATT 5 cut(s) 590, 606, 693, 757, 1182
MluNI TGGCCA 1 cut(s) 982
MlyI GAGTC 2 cut(s) 83, 210
MmeI TCCRAC 1 cut(s) 57
Mox20I TGGCCA 1 cut(s) 982
MscI TGGCCA 1 cut(s) 982
MseI TTAA 1 cut(s) 756
Msp20I TGGCCA 1 cut(s) 982
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 3 cut(s) 644, 880, 1013
MspR9I CCNGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
MunI CAATTG 1 cut(s) 1182
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
MvnI CGCG 3 cut(s) 148, 273, 930
MwoI GCNNNNNNNGC 8 cut(s) 152, 189, 350, 640, 655, 746, 975, 1187
NdeII GATC 3 cut(s) 54, 209, 684
NlaIII CATG 6 cut(s) 143, 236, 449, 503, 529, 1052
NlaIV GGNNCC 6 cut(s) 56, 137, 789, 1056, 1189, 1190
NspI RCATGY 2 cut(s) 236, 449
PaeI GCATGC 1 cut(s) 236
PaeR7I CTCGAG 1 cut(s) 837
PauI GCGCGC 1 cut(s) 271
PceI AGGCCT 1 cut(s) 1122
PciI ACATGT 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 844
PctI GAATGC 1 cut(s) 240
PfeI GAWTC 1 cut(s) 167
PflMI CCANNNNNTGG 2 cut(s) 593, 736
PfoI TCCNGGA 2 cut(s) 451, 801
PinAI ACCGGT 1 cut(s) 879
PkrI GCNGC 6 cut(s) 116, 157, 540, 860, 1011, 1030
PleI GAGTC 2 cut(s) 82, 209
PpsI GAGTC 2 cut(s) 82, 209
Ppu21I YACGTR 1 cut(s) 512
PscI ACATGT 1 cut(s) 445
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
PspEI GGTNACC 1 cut(s) 1036
PspFI CCCAGC 1 cut(s) 853
PspGI CCWGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
PspN4I GGNNCC 6 cut(s) 56, 137, 789, 1056, 1189, 1190
PspOMI GGGCCC 1 cut(s) 1188
PspPI GGNCC 4 cut(s) 1080, 1094, 1188, 1189
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 54
PteI GCGCGC 1 cut(s) 271
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 5 cut(s) 137, 151, 255, 530, 829
RsaNI GTAC 5 cut(s) 136, 150, 254, 529, 828
SacI GAGCTC 1 cut(s) 651
SalI GTCGAC 1 cut(s) 997
SaqAI TTAA 1 cut(s) 756
SatI GCNGC 6 cut(s) 115, 156, 539, 859, 1010, 1029
Sau3AI GATC 3 cut(s) 54, 209, 684
Sau96I GGNCC 4 cut(s) 1080, 1094, 1188, 1189
SchI GAGTC 2 cut(s) 83, 210
ScrFI CCNGG 7 cut(s) 453, 549, 803, 1072, 1084, 1098, 1124
SduI GDGCHC 6 cut(s) 480, 651, 838, 1104, 1171, 1192
SfaNI GCATC 1 cut(s) 185
Sfr274I CTCGAG 1 cut(s) 837
SinI GGWCC 1 cut(s) 1094
SlaI CTCGAG 1 cut(s) 837
SmlI CTYRAG 3 cut(s) 557, 688, 837
SmoI CTYRAG 3 cut(s) 557, 688, 837
SphI GCATGC 1 cut(s) 236
Sse9I AATT 5 cut(s) 590, 606, 693, 757, 1182
SseBI AGGCCT 1 cut(s) 1122
SsiI CCGC 6 cut(s) 52, 928, 935, 1010, 1028, 1031
SspMI CTAG 5 cut(s) 171, 180, 312, 750, 1143
SstI GAGCTC 1 cut(s) 651
StuI AGGCCT 1 cut(s) 1122
StyD4I CCNGG 7 cut(s) 451, 547, 801, 1070, 1082, 1096, 1122
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 2 cut(s) 259, 514
TaqI TCGA 4 cut(s) 683, 838, 909, 998
TasI AATT 5 cut(s) 590, 606, 693, 757, 1182
TatI WGTACW 1 cut(s) 827
TauI GCSGC 2 cut(s) 1012, 1031
TfiI GAWTC 1 cut(s) 167
Tru1I TTAA 1 cut(s) 756
Tru9I TTAA 1 cut(s) 756
TscAI CASTG 4 cut(s) 127, 432, 971, 1011
TseI GCWGC 4 cut(s) 114, 155, 538, 858
TspDTI ATGAA 2 cut(s) 350, 514
TspRI CASTG 4 cut(s) 127, 432, 971, 1011
Van91I CCANNNNNTGG 2 cut(s) 593, 736
VpaK11BI GGWCC 1 cut(s) 1094
XapI RAATTY 1 cut(s) 590
XceI RCATGY 2 cut(s) 236, 449
XcmI CCANNNNNNNNNTGG 1 cut(s) 990
XhoI CTCGAG 1 cut(s) 837
XmiI GTMKAC 2 cut(s) 508, 998
XspI CTAG 5 cut(s) 171, 180, 312, 750, 1143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.