Rmu_sc0004202.1_g000001

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004202.1
OG0016915
Physical Location & Seq
Reverse (-)
2131 .. 4083
1953 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004202.1_g000001.1.cds

Sequence Viewer

Length: 591 bp
atggcctcgcatacgcccatagaagagcttttgcgtgttgcgaaacgagttaactatccacaaattaactatccacaaactgatgccggtataataagaaacccccccccacctgatggcgagttcgtcgcttctgatcaagtctctaggtccttctctaggtggttcactgaaaccaaaggacgaaggttgcaaccatatttgaccgagctgacagatgctctcaaggtgtatgacatttcttgccaactgactgtgcaggtgataaagtacatcaatggaagttttgctgaagacatcctgattgggcatcaggaaggtgggctttgctcaacatatagaatcgaatcagagcaatctcttgagcggaagatactcctctctggttctttacgggaccttgaacgactgacggggtgtgatatttggctgggtgcgaaccgcgttattattatcggtacatttgaaggatcaaggctagcaaaggaggttgtggaagactgcatcgttcgtaatatgcctcctgcgcccaaaatcagagacctcatgacgagtctagcatcgcaggaattcgagactctgggtttatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

196

Amino Acids

22.06

Weight (kDa)

5.33

Isoelectric Point (pI)

57.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 250
AccB7I CCANNNNNTGG 1 cut(s) 116
AccBSI CCGCTC 1 cut(s) 367
AccII CGCG 1 cut(s) 444
AciI CCGC 2 cut(s) 367, 442
AclWI GGATC 1 cut(s) 478
AcsI RAATTY 1 cut(s) 569
AcuI CTGAAG 1 cut(s) 312
AfaI GTAC 2 cut(s) 272, 460
AfiI CCNNNNNNNGG 2 cut(s) 116, 159
AgsI TTSAA 2 cut(s) 404, 467
AluBI AGCT 2 cut(s) 28, 211
AluI AGCT 2 cut(s) 28, 211
Alw26I GTCTC 3 cut(s) 148, 534, 569
AlwI GGATC 1 cut(s) 478
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 569
AspLEI GCGC 1 cut(s) 529
AspS9I GGNCC 2 cut(s) 150, 397
AsuHPI GGTGA 1 cut(s) 274
AsuNHI GCTAGC 1 cut(s) 478
AvaII GGWCC 2 cut(s) 150, 397
BbsI GAAGAC 2 cut(s) 300, 504
BccI CCATC 1 cut(s) 110
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 3 cut(s) 148, 534, 569
BfaI CTAG 4 cut(s) 147, 159, 479, 557
BfuAI ACCTGC 1 cut(s) 250
Bme18I GGWCC 2 cut(s) 150, 397
BmgT120I GGNCC 2 cut(s) 150, 397
BmiI GGNNCC 1 cut(s) 398
BmsI GCATC 5 cut(s) 73, 208, 319, 513, 569
BmtI GCTAGC 1 cut(s) 482
BpiI GAAGAC 2 cut(s) 300, 504
BpuEI CTTGAG 2 cut(s) 209, 383
BsaI GGTCTC 1 cut(s) 534
Bsc4I CCNNNNNNNGG 2 cut(s) 116, 159
Bse118I RCCGGY 1 cut(s) 86
BseGI GGATG 1 cut(s) 297
BseLI CCNNNNNNNGG 2 cut(s) 116, 159
BseRI GAGGAG 1 cut(s) 368
BseYI CCCAGC 1 cut(s) 430
BsgI GTGCAG 1 cut(s) 278
Bsh1236I CGCG 1 cut(s) 444
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 2 cut(s) 116, 159
BsmAI GTCTC 3 cut(s) 148, 534, 569
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 534
Bsp143I GATC 2 cut(s) 136, 470
BspACI CCGC 2 cut(s) 367, 442
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 444
BspHI TCATGA 1 cut(s) 546
BspLI GGNNCC 1 cut(s) 398
BspMI ACCTGC 1 cut(s) 250
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 1 cut(s) 478
BspQI GCTCTTC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 534
BsrBI CCGCTC 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 86
BssAI RCCGGY 1 cut(s) 86
BssMI GATC 2 cut(s) 136, 470
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 480
BstENI CCTNNNNNAGG 1 cut(s) 157
BstF5I GGATG 1 cut(s) 297
BstFNI CGCG 1 cut(s) 444
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 2 cut(s) 139, 473
BstMAI GTCTC 3 cut(s) 148, 534, 569
BstMBI GATC 2 cut(s) 136, 470
BstMWI GCNNNNNNNGC 1 cut(s) 526
BstUI CGCG 1 cut(s) 444
BstV2I GAAGAC 2 cut(s) 300, 504
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 546
BtsCI GGATG 1 cut(s) 297
BtsIMutI CAGTG 1 cut(s) 168
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 480
CciI TCATGA 1 cut(s) 546
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 1 cut(s) 86
Cfr13I GGNCC 2 cut(s) 150, 397
Csp6I GTAC 2 cut(s) 271, 459
CviAII CATG 1 cut(s) 547
CviJI RGCY 6 cut(s) 5, 28, 211, 325, 430, 478
CviKI_1 RGCY 6 cut(s) 5, 28, 211, 325, 430, 478
CviQI GTAC 2 cut(s) 271, 459
DpnI GATC 2 cut(s) 138, 472
DpnII GATC 2 cut(s) 136, 470
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 534
Eco47I GGWCC 2 cut(s) 150, 397
Eco57I CTGAAG 1 cut(s) 312
EcoNI CCTNNNNNAGG 1 cut(s) 157
EcoO109I RGGNCCY 2 cut(s) 150, 397
EcoRI GAATTC 1 cut(s) 569
FaeI CATG 1 cut(s) 550
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 546
FbaI TGATCA 1 cut(s) 136
FokI GGATG 1 cut(s) 284
FspBI CTAG 4 cut(s) 147, 159, 479, 557
GlaI GCGC 1 cut(s) 528
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 87
HhaI GCGC 1 cut(s) 529
Hin1II CATG 1 cut(s) 550
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 4 cut(s) 342, 347, 553, 577
HpaI GTTAAC 1 cut(s) 52
HpaII CCGG 1 cut(s) 87
HphI GGTGA 1 cut(s) 274
Hpy166II GTNNAC 2 cut(s) 52, 168
Hpy188I TCNGA 3 cut(s) 136, 352, 539
Hpy188III TCNNGA 5 cut(s) 301, 314, 362, 547, 574
Hpy8I GTNNAC 2 cut(s) 52, 168
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 4 cut(s) 163, 180, 311, 461
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4V TGCA 3 cut(s) 193, 259, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 526
Hsp92II CATG 1 cut(s) 550
HspAI GCGC 1 cut(s) 527
Ksp22I TGATCA 1 cut(s) 136
KspAI GTTAAC 1 cut(s) 52
Kzo9I GATC 2 cut(s) 136, 470
LguI GCTCTTC 1 cut(s) 18
LweI GCATC 5 cut(s) 73, 208, 319, 513, 569
MaeI CTAG 4 cut(s) 147, 159, 479, 557
MalI GATC 2 cut(s) 138, 472
MbiI CCGCTC 1 cut(s) 367
MboI GATC 2 cut(s) 136, 470
MboII GAAGA 4 cut(s) 35, 305, 382, 509
MluCI AATT 2 cut(s) 63, 569
MlyI GAGTC 2 cut(s) 562, 571
MnlI CCTC 5 cut(s) 16, 389, 481, 531, 554
MseI TTAA 2 cut(s) 51, 66
MspI CCGG 1 cut(s) 87
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 526
NdeII GATC 2 cut(s) 136, 470
NheI GCTAGC 1 cut(s) 478
NlaIII CATG 1 cut(s) 550
NlaIV GGNNCC 1 cut(s) 398
PagI TCATGA 1 cut(s) 546
PaqCI CACCTGC 1 cut(s) 250
PciSI GCTCTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 342, 347
PflMI CCANNNNNTGG 1 cut(s) 116
PleI GAGTC 2 cut(s) 561, 571
PpsI GAGTC 2 cut(s) 561, 571
PpuMI RGGWCCY 2 cut(s) 150, 397
Psp5II RGGWCCY 2 cut(s) 150, 397
PspFI CCCAGC 1 cut(s) 430
PspN4I GGNNCC 1 cut(s) 398
PspPI GGNCC 2 cut(s) 150, 397
PspPPI RGGWCCY 2 cut(s) 150, 397
RsaI GTAC 2 cut(s) 272, 460
RsaNI GTAC 2 cut(s) 271, 459
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 51, 66
Sau3AI GATC 2 cut(s) 136, 470
Sau96I GGNCC 2 cut(s) 150, 397
SchI GAGTC 2 cut(s) 562, 571
SfaNI GCATC 5 cut(s) 73, 208, 319, 513, 569
SinI GGWCC 2 cut(s) 150, 397
SmlI CTYRAG 2 cut(s) 224, 362
SmoI CTYRAG 2 cut(s) 224, 362
Sse9I AATT 2 cut(s) 63, 569
SsiI CCGC 2 cut(s) 367, 442
SspMI CTAG 4 cut(s) 147, 159, 479, 557
TaaI ACNGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 345, 573
TaqII GACCGA 1 cut(s) 221
TasI AATT 2 cut(s) 63, 569
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 2 cut(s) 342, 347
Tru1I TTAA 2 cut(s) 51, 66
Tru9I TTAA 2 cut(s) 51, 66
TscAI CASTG 1 cut(s) 175
TspRI CASTG 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 116
VpaK11BI GGWCC 2 cut(s) 150, 397
XagI CCTNNNNNAGG 1 cut(s) 157
XapI RAATTY 1 cut(s) 569
XspI CTAG 4 cut(s) 147, 159, 479, 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.