Rroxscaffold_4G00294560

Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016915
Physical Location & Seq
Forward (+)
14616541 .. 14617239
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00294560.1

Sequence Viewer

Length: 669 bp
ATGGCCTTGCATACGCCCATAGAAGAGCTTTTGCGTGTTGCGAAGCGAATTAACTATCCACAAACTGATGGCGAACCCCCCCCCCCCCCACCTGATGGCGAGTTCGTCGCTTCTGATCAAGTCTCTAGGTCTTTCTCTAGGTGGTTCACTGAAACCAAAGGACGAAGGTTGCAACCATATTTGACCGAGCTGACAGATGCTCTCAAGGTGTATGACATTTCTTGCCAACTGACTCTGCAGCTTGACCAACACAGTGAGATGACATTCTCCACAAGCAACACAAACAGTGAAGCTAATGTTGCCAGGGCTAGGAAAGCTCTTGAATATTTGCAATTGATGCCATTTCTCTATACTCCTTACATCAATGGAAGCTTTGCTGAAGACATCCCGATTGGGCATCAGGAAGGTGGGCTTTGCTCAACATATAGAATCCAATCAGAGCAATCTCTTGAGCGGAAGATACTCCTCGCTGGTTCTTTACGGGACCTTGAACGACTGACGGGATGTGATATTTGGCTGGGTGCGAACCGCGTTATTATTATCGGTACACTTGAAGGATCAAGGCTAGCAAGGGAGGTTGTGGAAGACTGCATCGTTCGTAATATGCCTCCTGCGCCCAAAATCAGAGACCTCATGACGACTCTAGCATTCGAGACTTTGGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

222

Amino Acids

25.0

Weight (kDa)

5.2

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KH_KRR1_2nd PF21800 159 - 213 1e-07 KRR1 small subunit processome component, second KH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 95
AccBSI CCGCTC 1 cut(s) 454
AccII CGCG 1 cut(s) 531
AciI CCGC 2 cut(s) 454, 529
AclWI GGATC 1 cut(s) 565
AcuI CTGAAG 1 cut(s) 399
AfaI GTAC 1 cut(s) 547
AfiI CCNNNNNNNGG 2 cut(s) 95, 309
AgsI TTSAA 3 cut(s) 323, 491, 554
AjnI CCWGG 1 cut(s) 302
AluBI AGCT 6 cut(s) 28, 190, 241, 293, 317, 372
AluI AGCT 6 cut(s) 28, 190, 241, 293, 317, 372
Alw26I GTCTC 3 cut(s) 127, 621, 647
AlwI GGATC 1 cut(s) 565
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 1 cut(s) 484
AsuNHI GCTAGC 1 cut(s) 565
AvaII GGWCC 1 cut(s) 484
BbsI GAAGAC 2 cut(s) 387, 591
BbvI GCAGC 1 cut(s) 250
BccI CCATC 2 cut(s) 62, 89
BciT130I CCWGG 1 cut(s) 304
BclI TGATCA 1 cut(s) 115
BcoDI GTCTC 3 cut(s) 127, 621, 647
BfaI CTAG 5 cut(s) 126, 138, 309, 566, 644
BfmI CTRYAG 1 cut(s) 236
BisI GCNGC 1 cut(s) 239
BlsI GCNGC 1 cut(s) 240
Bme1390I CCNGG 1 cut(s) 304
Bme18I GGWCC 1 cut(s) 484
BmgT120I GGNCC 1 cut(s) 484
BmiI GGNNCC 1 cut(s) 485
BmrFI CCNGG 1 cut(s) 304
BmsI GCATC 4 cut(s) 187, 327, 406, 600
BmtI GCTAGC 1 cut(s) 569
BpiI GAAGAC 2 cut(s) 387, 591
BpuEI CTTGAG 2 cut(s) 188, 470
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 1 cut(s) 303
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 309
BseBI CCWGG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 303
BseGI GGATG 2 cut(s) 384, 509
BseLI CCNNNNNNNGG 2 cut(s) 95, 309
BseRI GAGGAG 1 cut(s) 455
BseXI GCAGC 1 cut(s) 250
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 1 cut(s) 531
BshFI GGCC 1 cut(s) 5
BslFI GGGAC 1 cut(s) 497
BslI CCNNNNNNNGG 2 cut(s) 95, 309
BsmAI GTCTC 3 cut(s) 127, 621, 647
BsmFI GGGAC 1 cut(s) 497
BsmI GAATGC 1 cut(s) 647
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 621
Bsp143I GATC 2 cut(s) 115, 557
BspACI CCGC 2 cut(s) 454, 529
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 531
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 485
BspMAI CTGCAG 1 cut(s) 240
BspOI GCTAGC 1 cut(s) 569
BspPI GGATC 1 cut(s) 565
BspQI GCTCTTC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 621
BsrBI CCGCTC 1 cut(s) 454
BssECI CCNNGG 1 cut(s) 303
BssMI GATC 2 cut(s) 115, 557
Bst2UI CCWGG 1 cut(s) 304
Bst4CI ACNGT 2 cut(s) 254, 287
Bst6I CTCTTC 1 cut(s) 18
BstAPI GCANNNNNTGC 1 cut(s) 337
BstC8I GCNNGC 1 cut(s) 567
BstF5I GGATG 2 cut(s) 384, 509
BstFNI CGCG 1 cut(s) 531
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 2 cut(s) 118, 560
BstMAI GTCTC 3 cut(s) 127, 621, 647
BstMBI GATC 2 cut(s) 115, 557
BstMWI GCNNNNNNNGC 4 cut(s) 299, 314, 337, 613
BstNI CCWGG 1 cut(s) 304
BstSCI CCNGG 1 cut(s) 302
BstSFI CTRYAG 1 cut(s) 236
BstUI CGCG 1 cut(s) 531
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 2 cut(s) 387, 591
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 2 cut(s) 384, 509
BtsIMutI CAGTG 3 cut(s) 147, 259, 292
Cac8I GCNNGC 1 cut(s) 567
CciI TCATGA 1 cut(s) 633
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 1 cut(s) 484
Csp6I GTAC 1 cut(s) 546
CviAII CATG 1 cut(s) 634
CviQI GTAC 1 cut(s) 546
DpnI GATC 2 cut(s) 117, 559
DpnII GATC 2 cut(s) 115, 557
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco31I GGTCTC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 484
Eco57I CTGAAG 1 cut(s) 399
EcoO109I RGGNCCY 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 302
FaeI CATG 1 cut(s) 637
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 1 cut(s) 497
FatI CATG 1 cut(s) 633
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 1 cut(s) 239
FokI GGATG 2 cut(s) 371, 516
Fsp4HI GCNGC 1 cut(s) 239
FspBI CTAG 5 cut(s) 126, 138, 309, 566, 644
GlaI GCGC 1 cut(s) 615
GluI GCNGC 1 cut(s) 239
GsaI CCCAGC 1 cut(s) 521
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 616
Hin1II CATG 1 cut(s) 637
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HindIII AAGCTT 1 cut(s) 370
HinfI GANTC 3 cut(s) 232, 429, 640
Hpy166II GTNNAC 2 cut(s) 147, 548
Hpy188I TCNGA 3 cut(s) 115, 439, 626
Hpy188III TCNNGA 6 cut(s) 320, 388, 401, 449, 634, 652
Hpy8I GTNNAC 2 cut(s) 147, 548
Hpy99I CGWCG 1 cut(s) 110
HpyAV CCTTC 3 cut(s) 159, 398, 548
HpyCH4III ACNGT 2 cut(s) 254, 287
HpyCH4V TGCA 5 cut(s) 10, 172, 238, 331, 591
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 314, 337, 613
Hsp92II CATG 1 cut(s) 637
HspAI GCGC 1 cut(s) 614
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 2 cut(s) 115, 557
LguI GCTCTTC 1 cut(s) 18
LpnPI CCDG 7 cut(s) 105, 289, 316, 386, 456, 503, 624
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 4 cut(s) 187, 327, 406, 600
MaeI CTAG 5 cut(s) 126, 138, 309, 566, 644
MalI GATC 2 cut(s) 117, 559
MbiI CCGCTC 1 cut(s) 454
MboI GATC 2 cut(s) 115, 557
MboII GAAGA 4 cut(s) 35, 392, 469, 596
MfeI CAATTG 1 cut(s) 332
MluCI AATT 2 cut(s) 48, 332
MlyI GAGTC 2 cut(s) 226, 634
MnlI CCTC 4 cut(s) 476, 568, 618, 641
MseI TTAA 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 304
MunI CAATTG 1 cut(s) 332
Mva1269I GAATGC 1 cut(s) 647
MvaI CCWGG 1 cut(s) 304
MvnI CGCG 1 cut(s) 531
MwoI GCNNNNNNNGC 4 cut(s) 299, 314, 337, 613
NdeII GATC 2 cut(s) 115, 557
NheI GCTAGC 1 cut(s) 565
NlaIII CATG 1 cut(s) 637
NlaIV GGNNCC 1 cut(s) 485
PagI TCATGA 1 cut(s) 633
PciSI GCTCTTC 1 cut(s) 18
PctI GAATGC 1 cut(s) 647
PfeI GAWTC 1 cut(s) 429
PflMI CCANNNNNTGG 1 cut(s) 95
PkrI GCNGC 1 cut(s) 240
PleI GAGTC 2 cut(s) 226, 634
PpsI GAGTC 2 cut(s) 226, 634
PpuMI RGGWCCY 1 cut(s) 484
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 1 cut(s) 302
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 1 cut(s) 302
PspN4I GGNNCC 1 cut(s) 485
PspPI GGNCC 1 cut(s) 484
PspPPI RGGWCCY 1 cut(s) 484
PstI CTGCAG 1 cut(s) 240
RsaI GTAC 1 cut(s) 547
RsaNI GTAC 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 1 cut(s) 239
Sau3AI GATC 2 cut(s) 115, 557
Sau96I GGNCC 1 cut(s) 484
SchI GAGTC 2 cut(s) 226, 634
ScrFI CCNGG 1 cut(s) 304
SfaNI GCATC 4 cut(s) 187, 327, 406, 600
SfcI CTRYAG 1 cut(s) 236
SinI GGWCC 1 cut(s) 484
SmlI CTYRAG 2 cut(s) 203, 449
SmoI CTYRAG 2 cut(s) 203, 449
Sse9I AATT 2 cut(s) 48, 332
SsiI CCGC 2 cut(s) 454, 529
SspI AATATT 1 cut(s) 326
SspMI CTAG 5 cut(s) 126, 138, 309, 566, 644
StyD4I CCNGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 254, 287
TaqI TCGA 1 cut(s) 651
TaqII GACCGA 1 cut(s) 200
TasI AATT 2 cut(s) 48, 332
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 3 cut(s) 154, 259, 292
TseI GCWGC 1 cut(s) 238
TspRI CASTG 3 cut(s) 154, 259, 292
Van91I CCANNNNNTGG 1 cut(s) 95
VpaK11BI GGWCC 1 cut(s) 484
XspI CTAG 5 cut(s) 126, 138, 309, 566, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.