Rmu_sc0004514.1_g000027

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004514.1
OG0016663
Physical Location & Seq
Forward (+)
117023 .. 117862
840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004514.1_g000027.1.cds

Sequence Viewer

Length: 840 bp
atgggggcaacgctggtaatggtggtgagccttgccatagtcttgggcttaatcttggttctcctagctgagctatactgttcactcttgctccgccgcaggcgtcagcacaaaaccgacagaaacaactccatcgtcaacaccgacctgtccaccaccgccgcctcagccaccgaagcagccaccaccacctctgccgagccctcattacaaccccaaggccctcctctaggcagctttttaggcgttcttcgagctccgagaagcttcctcttcccatcatgcaaagaagagtacaatgcagaaccaaagaagcaacaacactgtcacctccaccacgttctcgacatcccagaccaagaaatatcaaacgaatcaccatctatttccttagcaaattcaccacagccggcgcaacgagacccagttcaagctggtactactccaaacactgccgctgctgccggagaagatcatttggtatacatttccaatccgatatacgacaacgaagcaaacagagctagcagcagcactactacaggagcaaacactccctttgaaactccggacacatcgccttcaagacttgaaatggggggctctacttctagctccggtgaagataacgatgaggttgcacaaccaactccatccggttctggaccttcaagcccaaccacacctcctctaactccaatgaagaagctacccaaagaggcatgctctgtttctctcagagacgcaaggtctctaggaacttcaggtagtgattccaacactaacaatggtaactcatcctcatcctcaggttctccttgtacttctccttcatggtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

29.21

Weight (kDa)

5.23

Isoelectric Point (pI)

62.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016663)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G29300 AT3G29300
fragaria_vesca FvH4_1g04931
malus_domestica MD02G1047900.v1.1
prunus_persica Prupe.7G230300_v2.0.a1
pyrus_communis pycom15g16710
rosa_chinensis RchiOBHm_Chr2g0090531
rosa_laevigata RLG00000016120
rosa_multiflora Rmu_sc0004514.1_g000027
rosa_roxburghii Rroxscaffold_2G00151060
rosa_rugosa Rorug02G0008900
rosa_samantha Rh2AG054100 Rh2DG054300
rosa_wichuraiana Rw2G004770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 5 cut(s) 94, 97, 159, 162, 456
AcsI RAATTY 1 cut(s) 397
AcuI CTGAAG 1 cut(s) 747
AcyI GRCGYC 1 cut(s) 103
AfaI GTAC 3 cut(s) 296, 439, 823
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 5 cut(s) 431, 563, 585, 593, 672
AhdI GACNNNNNGTC 1 cut(s) 748
AluBI AGCT 9 cut(s) 68, 73, 237, 257, 267, 434, 524, 615, 709
AluI AGCT 9 cut(s) 68, 73, 237, 257, 267, 434, 524, 615, 709
Alw21I GWGCWC 1 cut(s) 259
Alw26I GTCTC 3 cut(s) 414, 735, 756
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 6 cut(s) 179, 234, 458, 461, 528, 531
ApoI RAATTY 1 cut(s) 397
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 221, 665
AsuHPI GGTGA 5 cut(s) 37, 320, 369, 393, 632
AsuNHI GCTAGC 1 cut(s) 524
AvaII GGWCC 1 cut(s) 665
AxyI CCTNAGG 1 cut(s) 808
BanII GRGCYC 3 cut(s) 204, 259, 605
BarI GAAGNNNNNNTAC 1 cut(s) 814
Bbv12I GWGCWC 1 cut(s) 259
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 6 cut(s) 191, 246, 445, 448, 540, 543
BccI CCATC 4 cut(s) 140, 286, 388, 661
BcgI CGANNNNNNTGC 2 cut(s) 620, 654
BcoDI GTCTC 3 cut(s) 414, 735, 756
BfaI CTAG 5 cut(s) 65, 230, 525, 612, 755
BfmI CTRYAG 1 cut(s) 540
BisI GCNGC 9 cut(s) 97, 162, 180, 235, 456, 459, 462, 529, 532
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 9 cut(s) 98, 163, 181, 236, 457, 460, 463, 530, 533
Bme18I GGWCC 1 cut(s) 665
BmeRI GACNNNNNGTC 1 cut(s) 748
BmgT120I GGNCC 2 cut(s) 221, 665
BmrI ACTGGG 1 cut(s) 419
BmtI GCTAGC 1 cut(s) 528
BmuI ACTGGG 1 cut(s) 419
BplI GAGNNNNNCTC 2 cut(s) 710, 742
Bpu10I CCTNAGC 2 cut(s) 166, 391
Bpu1102I GCTNAGC 1 cut(s) 69
BsaHI GRCGYC 1 cut(s) 103
BsaI GGTCTC 2 cut(s) 414, 756
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 3 cut(s) 568, 617, 656
BsaXI ACNNNNNCTCC 4 cut(s) 75, 105, 670, 700
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 1 cut(s) 425
Bse21I CCTNAGG 1 cut(s) 808
BseAI TCCGGA 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 4 cut(s) 348, 653, 797, 803
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 4 cut(s) 60, 180, 751, 822
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 2 cut(s) 216, 678
BseXI GCAGC 6 cut(s) 191, 246, 445, 448, 540, 543
BshFI GGCC 1 cut(s) 222
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 5 cut(s) 410, 465, 569, 618, 657
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 3 cut(s) 414, 735, 756
BsmBI CGTCTC 1 cut(s) 735
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 2 cut(s) 414, 756
Bsp1286I GDGCHC 3 cut(s) 204, 259, 605
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 1 cut(s) 472
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 5 cut(s) 94, 97, 159, 162, 456
BspANI GGCC 1 cut(s) 222
BspCNI CTCAG 4 cut(s) 61, 179, 750, 821
BspEI TCCGGA 1 cut(s) 568
BspOI GCTAGC 1 cut(s) 528
BspTNI GGTCTC 2 cut(s) 414, 756
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 409
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 1 cut(s) 472
BssNAI GTATAC 1 cut(s) 484
BssNI GRCGYC 1 cut(s) 103
BssT1I CCWWGG 1 cut(s) 217
Bst1107I GTATAC 1 cut(s) 484
Bst4CI ACNGT 2 cut(s) 80, 326
Bst6I CTCTTC 2 cut(s) 278, 285
BstACI GRCGYC 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 101, 411, 526, 724
BstDEI CTNAG 5 cut(s) 69, 166, 391, 737, 808
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 4 cut(s) 348, 653, 797, 803
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 1 cut(s) 475
BstMAI GTCTC 3 cut(s) 414, 735, 756
BstMBI GATC 1 cut(s) 472
BstMWI GCNNNNNNNGC 5 cut(s) 167, 176, 243, 461, 521
BstNSI RCATGY 1 cut(s) 726
BstSFI CTRYAG 1 cut(s) 540
BstV1I GCAGC 6 cut(s) 191, 246, 445, 448, 540, 543
BstXI CCANNNNNNTGG 1 cut(s) 43
BstZ17I GTATAC 1 cut(s) 484
Bsu36I CCTNAGG 1 cut(s) 808
BsuRI GGCC 1 cut(s) 222
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 4 cut(s) 348, 653, 797, 803
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 2 cut(s) 322, 450
Cac8I GCNNGC 4 cut(s) 101, 411, 526, 724
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 409
Cfr13I GGNCC 2 cut(s) 221, 665
CseI GACGC 2 cut(s) 92, 752
Csp6I GTAC 3 cut(s) 295, 438, 822
CviAII CATG 3 cut(s) 282, 723, 834
CviQI GTAC 3 cut(s) 295, 438, 822
DdeI CTNAG 5 cut(s) 69, 166, 391, 737, 808
DpnI GATC 1 cut(s) 474
DpnII GATC 1 cut(s) 472
DriI GACNNNNNGTC 1 cut(s) 748
Eam1104I CTCTTC 2 cut(s) 278, 285
Eam1105I GACNNNNNGTC 1 cut(s) 748
EarI CTCTTC 2 cut(s) 278, 285
EciI GGCGGA 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 3 cut(s) 204, 259, 605
Eco31I GGTCTC 2 cut(s) 414, 756
Eco47I GGWCC 1 cut(s) 665
Eco53kI GAGCTC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 747
Eco81I CCTNAGG 1 cut(s) 808
EcoICRI GAGCTC 1 cut(s) 257
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 3 cut(s) 204, 259, 605
ErhI CCWWGG 1 cut(s) 217
Esp3I CGTCTC 1 cut(s) 735
FaeI CATG 3 cut(s) 285, 726, 837
FaiI YATR 7 cut(s) 38, 76, 283, 484, 502, 724, 835
FatI CATG 3 cut(s) 281, 722, 833
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 9 cut(s) 97, 162, 180, 235, 456, 459, 462, 529, 532
FokI GGATG 4 cut(s) 335, 640, 784, 790
FriOI GRGCYC 3 cut(s) 204, 259, 605
Fsp4HI GCNGC 9 cut(s) 97, 162, 180, 235, 456, 459, 462, 529, 532
FspBI CTAG 5 cut(s) 65, 230, 525, 612, 755
GlaI GCGC 1 cut(s) 414
GluI GCNGC 9 cut(s) 97, 162, 180, 235, 456, 459, 462, 529, 532
HaeIII GGCC 1 cut(s) 222
HapII CCGG 5 cut(s) 410, 465, 569, 618, 657
HgaI GACGC 2 cut(s) 92, 752
HhaI GCGC 1 cut(s) 415
Hin1I GRCGYC 1 cut(s) 103
Hin1II CATG 3 cut(s) 285, 726, 837
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HincII GTYRAC 1 cut(s) 139
HindII GTYRAC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 2 cut(s) 374, 773
HpaII CCGG 5 cut(s) 410, 465, 569, 618, 657
HphI GGTGA 5 cut(s) 37, 320, 369, 393, 632
Hpy166II GTNNAC 4 cut(s) 83, 139, 153, 484
Hpy188I TCNGA 3 cut(s) 261, 498, 740
Hpy188III TCNNGA 4 cut(s) 344, 569, 585, 663
Hpy8I GTNNAC 4 cut(s) 83, 139, 153, 484
HpyAV CCTTC 3 cut(s) 591, 678, 840
HpyCH4III ACNGT 2 cut(s) 80, 326
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 3 cut(s) 285, 302, 641
HpyF10VI GCNNNNNNNGC 5 cut(s) 167, 176, 243, 461, 521
HpyF3I CTNAG 5 cut(s) 69, 166, 391, 737, 808
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 103
Hsp92II CATG 3 cut(s) 285, 726, 837
HspAI GCGC 1 cut(s) 413
Kpn2I TCCGGA 1 cut(s) 568
KroI GCCGGC 1 cut(s) 409
KroNI GCCGGC 1 cut(s) 411
Kzo9I GATC 1 cut(s) 472
LmnI GCTCC 4 cut(s) 96, 262, 545, 620
Lsp1109I GCAGC 6 cut(s) 191, 246, 445, 448, 540, 543
MaeI CTAG 5 cut(s) 65, 230, 525, 612, 755
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 2 cut(s) 326, 791
MalI GATC 1 cut(s) 474
MboI GATC 1 cut(s) 472
MboII GAAGA 6 cut(s) 242, 265, 302, 482, 635, 715
MhlI GDGCHC 3 cut(s) 204, 259, 605
MluCI AATT 1 cut(s) 397
MmeI TCCRAC 1 cut(s) 801
MroI TCCGGA 1 cut(s) 568
MroNI GCCGGC 1 cut(s) 409
MseI TTAA 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 458
MspI CCGG 5 cut(s) 410, 465, 569, 618, 657
MwoI GCNNNNNNNGC 5 cut(s) 167, 176, 243, 461, 521
NaeI GCCGGC 1 cut(s) 411
NdeII GATC 1 cut(s) 472
NgoMIV GCCGGC 1 cut(s) 409
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 3 cut(s) 285, 726, 837
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 326
NspI RCATGY 1 cut(s) 726
PaeI GCATGC 1 cut(s) 726
PcsI WCGNNNNNNNCGW 1 cut(s) 141
PdiI GCCGGC 1 cut(s) 411
PfeI GAWTC 2 cut(s) 374, 773
PkrI GCNGC 9 cut(s) 98, 163, 181, 236, 457, 460, 463, 530, 533
Psp124BI GAGCTC 1 cut(s) 259
PspPI GGNCC 2 cut(s) 221, 665
RsaI GTAC 3 cut(s) 296, 439, 823
RsaNI GTAC 3 cut(s) 295, 438, 822
SacI GAGCTC 1 cut(s) 259
SaqAI TTAA 1 cut(s) 50
SatI GCNGC 9 cut(s) 97, 162, 180, 235, 456, 459, 462, 529, 532
Sau3AI GATC 1 cut(s) 472
Sau96I GGNCC 2 cut(s) 221, 665
SduI GDGCHC 3 cut(s) 204, 259, 605
SfcI CTRYAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 665
SphI GCATGC 1 cut(s) 726
Sse9I AATT 1 cut(s) 397
SsiI CCGC 5 cut(s) 94, 97, 159, 162, 456
SspMI CTAG 5 cut(s) 65, 230, 525, 612, 755
SstI GAGCTC 1 cut(s) 259
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 2 cut(s) 80, 326
TaiI ACGT 1 cut(s) 342
TaqI TCGA 2 cut(s) 253, 345
TasI AATT 1 cut(s) 397
TatI WGTACW 2 cut(s) 294, 821
TauI GCSGC 3 cut(s) 99, 164, 458
TfiI GAWTC 2 cut(s) 374, 773
Tru1I TTAA 1 cut(s) 50
Tru9I TTAA 1 cut(s) 50
TscAI CASTG 2 cut(s) 329, 457
TseFI GTSAC 1 cut(s) 326
TseI GCWGC 6 cut(s) 179, 234, 458, 461, 528, 531
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 2 cut(s) 716, 822
TspRI CASTG 2 cut(s) 329, 457
VpaK11BI GGWCC 1 cut(s) 665
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 1 cut(s) 397
XceI RCATGY 1 cut(s) 726
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 5 cut(s) 65, 230, 525, 612, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.