Rmu_sc0004628.1_g000014

Photosystem II reaction center PSB28 protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004628.1
OG0010122
Physical Location & Seq
Reverse (-)
36103 .. 38527
2425 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 729 bp
atgatgcgtggggtggtacagcaactccagatagttctgatgatggtggagagggtggaagcggaacaggtggtggaggtgaaagatatgaatatggacaatcttctgtggatgaaggattcaaatttacctgtgaaggtaattttgatcatgtcacccaagatgaagaccacagattgtgcaatggcaaccttgcagtcttcacttgcattctcctctccagcctcccactgtctccagcaatcacgctctcattctgggttgttatcttgggcggttcatagaagtgcagagtccacattcaatggccagtctttgcgcatttctcggccccaatgggcgccactaaggtggtctagtcaagcatctagatccatcaaaatgatggcaaaaccaaaaattcagtttatccaaggaactgatgagcagacaataccagatgtgaggctaaccaaatcaaaagatggtaccaatggcgtggctatattcaggtttgagcagccttctgttttcgactcatctggcgaatttggtgatatcactggcttttacatgattgatgaagaaggggtcatttcatctgttgatgttaacgcaaaatttgtcaatggaaagcctgcaggaattgaagcaaagtatataatgcggacaccaagtgaatgggacagattcatgagattcatggagcgctatgctaatgacaatggcttgagtttcattaaaaaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
Psb28

Protein Analysis

242

Amino Acids

27.35

Weight (kDa)

8.85

Isoelectric Point (pI)

58.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Psb28 PF03912 134 - 239 1e-42 Psb28 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 320
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 2 cut(s) 340, 467
AciI CCGC 3 cut(s) 62, 275, 646
AclWI GGATC 1 cut(s) 366
AcoI YGGCCR 1 cut(s) 307
AcsI RAATTY 4 cut(s) 124, 399, 527, 599
AcyI GRCGYC 1 cut(s) 341
AdeI CACNNNGTG 1 cut(s) 656
AfaI GTAC 2 cut(s) 18, 469
AfeI AGCGCT 1 cut(s) 689
AgsI TTSAA 3 cut(s) 123, 304, 629
AleI CACNNNNGTG 1 cut(s) 349
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 366
Aor51HI AGCGCT 1 cut(s) 689
AoxI GGCC 2 cut(s) 307, 329
ApeKI GCWGC 1 cut(s) 499
ApoI RAATTY 4 cut(s) 124, 399, 527, 599
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 3 cut(s) 321, 343, 690
AspS9I GGNCC 1 cut(s) 330
AsuHPI GGTGA 3 cut(s) 91, 147, 545
BalI TGGCCA 1 cut(s) 309
BanI GGYRCC 2 cut(s) 340, 467
BbsI GAAGAC 2 cut(s) 173, 192
BbvI GCAGC 1 cut(s) 511
BccI CCATC 4 cut(s) 37, 379, 383, 458
BclI TGATCA 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 239
BfaI CTAG 2 cut(s) 357, 369
BfmI CTRYAG 1 cut(s) 618
BfoI RGCGCY 2 cut(s) 344, 691
BisI GCNGC 1 cut(s) 500
BlsI GCNGC 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 330
BmiI GGNNCC 3 cut(s) 332, 342, 469
BmsI GCATC 1 cut(s) 374
BpiI GAAGAC 2 cut(s) 173, 192
BpmI CTGGAG 3 cut(s) 11, 204, 221
BsaHI GRCGYC 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 412
BsaXI ACNNNNNCTCC 2 cut(s) 9, 39
Bse1I ACTGG 2 cut(s) 310, 547
Bse3DI GCAATG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 412
BseGI GGATG 1 cut(s) 117
BseMI GCAATG 1 cut(s) 189
BseNI ACTGG 2 cut(s) 310, 547
BseRI GAGGAG 1 cut(s) 205
BseXI GCAGC 1 cut(s) 511
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 2 cut(s) 309, 331
BshNI GGYRCC 2 cut(s) 340, 467
BslFI GGGAC 1 cut(s) 677
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 209
BsnI GGCC 2 cut(s) 309, 331
Bsp143I GATC 2 cut(s) 147, 371
BspACI CCGC 3 cut(s) 62, 275, 646
BspANI GGCC 2 cut(s) 309, 331
BspHI TCATGA 1 cut(s) 672
BspLI GGNNCC 3 cut(s) 332, 342, 469
BspMAI CTGCAG 1 cut(s) 622
BspPI GGATC 1 cut(s) 366
BspT107I GGYRCC 2 cut(s) 340, 467
BsrDI GCAATG 1 cut(s) 189
BsrI ACTGG 2 cut(s) 310, 547
BssECI CCNNGG 1 cut(s) 412
BssMI GATC 2 cut(s) 147, 371
BssNI GRCGYC 1 cut(s) 341
BssT1I CCWWGG 1 cut(s) 412
Bst4CI ACNGT 1 cut(s) 233
BstACI GRCGYC 1 cut(s) 341
BstC8I GCNNGC 1 cut(s) 618
BstDEI CTNAG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 117
BstH2I RGCGCY 2 cut(s) 344, 691
BstHHI GCGC 3 cut(s) 321, 343, 690
BstKTI GATC 2 cut(s) 150, 374
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 147, 371
BstSFI CTRYAG 1 cut(s) 618
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 2 cut(s) 173, 192
BstX2I RGATCY 1 cut(s) 371
BstXI CCANNNNNNTGG 3 cut(s) 351, 478, 660
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 2 cut(s) 309, 331
BtsCI GGATG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 229, 540
Cac8I GCNNGC 1 cut(s) 618
CciI TCATGA 1 cut(s) 672
CfoI GCGC 3 cut(s) 321, 343, 690
Cfr13I GGNCC 1 cut(s) 330
Csp6I GTAC 2 cut(s) 17, 468
CviAII CATG 4 cut(s) 151, 553, 673, 682
CviJI RGCY 9 cut(s) 224, 309, 331, 448, 482, 502, 546, 616, 708
CviKI_1 RGCY 9 cut(s) 224, 309, 331, 448, 482, 502, 546, 616, 708
CviQI GTAC 2 cut(s) 17, 468
DdeI CTNAG 1 cut(s) 347
DinI GGCGCC 1 cut(s) 342
DpnI GATC 2 cut(s) 149, 373
DpnII GATC 2 cut(s) 147, 371
DraIII CACNNNGTG 1 cut(s) 656
EaeI YGGCCR 1 cut(s) 307
Eco130I CCWWGG 1 cut(s) 412
Eco32I GATATC 1 cut(s) 538
Eco47III AGCGCT 1 cut(s) 689
EcoRV GATATC 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 412
EgeI GGCGCC 1 cut(s) 342
EheI GGCGCC 1 cut(s) 342
ErhI CCWWGG 1 cut(s) 412
FaeI CATG 4 cut(s) 154, 556, 676, 685
FaqI GGGAC 1 cut(s) 677
FatI CATG 4 cut(s) 150, 552, 672, 681
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 1 cut(s) 500
FokI GGATG 1 cut(s) 124
Fsp4HI GCNGC 1 cut(s) 500
FspBI CTAG 2 cut(s) 357, 369
FspI TGCGCA 1 cut(s) 320
GlaI GCGC 3 cut(s) 320, 342, 689
GluI GCNGC 1 cut(s) 500
GsuI CTGGAG 3 cut(s) 11, 204, 221
HaeII RGCGCY 2 cut(s) 344, 691
HaeIII GGCC 2 cut(s) 309, 331
HhaI GCGC 3 cut(s) 321, 343, 690
Hin1I GRCGYC 1 cut(s) 341
Hin1II CATG 4 cut(s) 154, 556, 676, 685
Hin6I GCGC 3 cut(s) 319, 341, 688
HinP1I GCGC 3 cut(s) 319, 341, 688
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 5 cut(s) 119, 293, 515, 669, 678
HpaI GTTAAC 1 cut(s) 592
HphI GGTGA 3 cut(s) 91, 147, 545
Hpy166II GTNNAC 2 cut(s) 297, 592
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 3 cut(s) 28, 369, 673
Hpy8I GTNNAC 2 cut(s) 297, 592
HpyAV CCTTC 4 cut(s) 109, 130, 513, 560
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4V TGCA 5 cut(s) 182, 196, 209, 290, 620
HpyF3I CTNAG 1 cut(s) 347
Hsp92I GRCGYC 1 cut(s) 341
Hsp92II CATG 4 cut(s) 154, 556, 676, 685
HspAI GCGC 3 cut(s) 319, 341, 688
KasI GGCGCC 1 cut(s) 340
KpnI GGTACC 1 cut(s) 471
Ksp22I TGATCA 1 cut(s) 147
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 2 cut(s) 147, 371
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 1 cut(s) 374
MaeI CTAG 2 cut(s) 357, 369
MaeIII GTNAC 1 cut(s) 153
MalI GATC 2 cut(s) 149, 373
MboI GATC 2 cut(s) 147, 371
MboII GAAGA 4 cut(s) 95, 178, 192, 575
MflI RGATCY 1 cut(s) 371
MlsI TGGCCA 1 cut(s) 309
MluCI AATT 6 cut(s) 124, 141, 399, 527, 599, 624
MluNI TGGCCA 1 cut(s) 309
Mly113I GGCGCC 1 cut(s) 341
MlyI GAGTC 2 cut(s) 302, 509
MnlI CCTC 5 cut(s) 45, 70, 226, 235, 438
Mox20I TGGCCA 1 cut(s) 309
MscI TGGCCA 1 cut(s) 309
MseI TTAA 2 cut(s) 591, 720
MslI CAYNNNNRTG 3 cut(s) 285, 349, 380
Msp20I TGGCCA 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 209
NarI GGCGCC 1 cut(s) 341
NdeII GATC 2 cut(s) 147, 371
NlaIII CATG 4 cut(s) 154, 556, 676, 685
NlaIV GGNNCC 3 cut(s) 332, 342, 469
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 153
NsbI TGCGCA 1 cut(s) 320
OliI CACNNNNGTG 1 cut(s) 349
PagI TCATGA 1 cut(s) 672
PctI GAATGC 1 cut(s) 209
PfeI GAWTC 3 cut(s) 119, 669, 678
PkrI GCNGC 1 cut(s) 501
PleI GAGTC 2 cut(s) 301, 509
PluTI GGCGCC 1 cut(s) 344
PpsI GAGTC 2 cut(s) 301, 509
PspN4I GGNNCC 3 cut(s) 332, 342, 469
PspPI GGNCC 1 cut(s) 330
PstI CTGCAG 1 cut(s) 622
PsuI RGATCY 1 cut(s) 371
RsaI GTAC 2 cut(s) 18, 469
RsaNI GTAC 2 cut(s) 17, 468
RseI CAYNNNNRTG 3 cut(s) 285, 349, 380
SaqAI TTAA 2 cut(s) 591, 720
SatI GCNGC 1 cut(s) 500
Sau3AI GATC 2 cut(s) 147, 371
Sau96I GGNCC 1 cut(s) 330
SbfI CCTGCAGG 1 cut(s) 622
SchI GAGTC 2 cut(s) 302, 509
SdaI CCTGCAGG 1 cut(s) 622
SetI ASST 7 cut(s) 72, 81, 133, 141, 194, 353, 494
SfaNI GCATC 1 cut(s) 374
SfcI CTRYAG 1 cut(s) 618
SfoI GGCGCC 1 cut(s) 342
SmiMI CAYNNNNRTG 3 cut(s) 285, 349, 380
SmlI CTYRAG 1 cut(s) 709
SmoI CTYRAG 1 cut(s) 709
Sse8387I CCTGCAGG 1 cut(s) 622
Sse9I AATT 6 cut(s) 124, 141, 399, 527, 599, 624
SsiI CCGC 3 cut(s) 62, 275, 646
SspDI GGCGCC 1 cut(s) 340
SspMI CTAG 2 cut(s) 357, 369
StyI CCWWGG 1 cut(s) 412
TaaI ACNGT 1 cut(s) 233
TaqI TCGA 1 cut(s) 513
TasI AATT 6 cut(s) 124, 141, 399, 527, 599, 624
TfiI GAWTC 3 cut(s) 119, 669, 678
Tru1I TTAA 2 cut(s) 591, 720
Tru9I TTAA 2 cut(s) 591, 720
TscAI CASTG 2 cut(s) 236, 547
TseFI GTSAC 1 cut(s) 153
TseI GCWGC 1 cut(s) 499
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 9 cut(s) 104, 128, 179, 269, 567, 576, 661, 670, 706
TspRI CASTG 2 cut(s) 236, 547
XapI RAATTY 4 cut(s) 124, 399, 527, 599
XbaI TCTAGA 1 cut(s) 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 382
XspI CTAG 2 cut(s) 357, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.