Rmu_sc0004644.1_g000018

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004644.1
OG0009414
Physical Location & Seq
Forward (+)
113958 .. 127151
13194 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3420 bp
atgattgggggcggcaagagctacgtctcagccccaccggccttctccaacgacgccaagcgcctcctcgtctgcaccggcaccgccgtctccgtcttcagcacctccaccggcttacagatactgtcgttggagggccacacggcgttggtcaccgccgtcatagtggttcccggtaacaaagccctcagcttctgctggacggcctcgcttgacggcaccgttaggtattgggactttgccgccgccgagttgatgaagaccattgatatcaaaatgcctatttactccatggttattccggcattgttgttgggccaaacggattcgaaggtgatgccggccgatgcttttgcttatttgtctgttgaaaacaccacccaagacaatgcacccaaggcttcgcgtgggcagattcgcaagtgtaatttgactgagtctcgattggctggtgttattctcgcagagactagaaaaccagaagttatatctatcagtccctcaggaaaattctttggcatccggaacaagcggaagcttcatatctggagggcccctgctaaagattctaaactcccagtggccaagattacgctacaccatacaagaaattttacacttcttgcatttcatccaacaaagaggctagttgctgcaggtgatacatctgggagaattctaatctggagagggtttggtaaccgtaaattttcagatggtgccaaactagtaaatcggggatctatgaatgatgaggaaggaagacctgggatcaggggagatgatgatgctgactcttgcaccacttggcattggcatcctactggaattaatctcctgcgtttttcttctgatggagcgtatttatattcaggtggaaaggaaggagttcttgtggactggcagctagacacagggaaaaagaaatttttaccaagaattggatctccacttctatatttaactgattctctagatccttcactttctgctctatcttgtgcagataatcaaattcacttgctaaaaatgccctcaatggaaatcacaaagtctatttcagggatcaagctcccgttttcttctccggatgtatatgaaggcatgtgcagcaagtttgccttcgatgacactgatgggttagttgccttgcgtacagagaactatcgcatccagttttacagcttgtttaatgaccgtgaaatttctgaggttcaagtctgtgagagaaaccatcaaccgggcgatgaagtctctgtaatagtgactttggtgtctctgtctgaagatggctctatgatgagcacagtggaagttaatcttcctgaggatggaataggtggtcttgtgtgtcttaagttctgggcatcagattcgcagaacaaaaatttcaacttgtccaccattgtgtatcaacctcacagggatgctggagtttcttctcttgcttttcatcctacccgtcaaatggttgtcagttcatcatatggtggggatttcaagatttgggtttgcagtgacgagattcagccgaaacaagatacactacagcattctgggtggatgtgccatgctgtaggttcatacaagaaaaagccaatgacagctgctgcattttctgccgatggttctgttctggctgttgcagctgaaactgtaatcacactatgggaccctgtgaacaacatgcttgtggctgtgcttggagagatgcagacgccaattgtgaccctcttctttgctgggaattcggactatcttttgtctatctcccggggttcaaaaccacagttatctgtgtggagcatgtcaaagctgtctgagtcttggtcatacaagcttgacatagaagatgttacgtgtgcgaaagattcatcatcatttgcagttcttgcccttttgccaaaatcatctacttttatgggctctaatgaatcaatttttcaaggaaaagatggggtaatcttattattcaatgcaacagatcctgttccgttggctatgtggtccgtaaggaaggtcaagggagggggacttgctttccttaaagggaactcctttgaacaacaaagtatagcggatggaaaatcaccacaagctttgcttgcctatataaatgccgatcatgagtacatcctctttgatccatatgactacgatgcacgtgaacttagtatgaccagggggaaggatcgtgttgccgttgacgaaacagcgcaatatggatatgcatctatctatggggaactaccgcaatttgagatgaagagagaccagaccttgttagccccatatgctccatcacaaaggccttgggagaccatttttaaagagccaccaacaatggcaccagaaaaccttcaatgtcttaagaaatatatcggtccattattgtgtttgcatgtagttcttttgtcaagtttctgtgggattgctacctcccaaaccatagtcaccagtttacctggttttgaaggggacctgccattcacacttgaaactgggtatgtgggtgtgggtgactcaaactcctcccaattgttctactattttgttgagtcgacgagaagcccttccgaagaccctcttatgctttggttcactggaggccctggctgctctgttctctctgccttcttttatgaaagtggtccactatctttcacatatgtagattacaatggaagtctaccctcacttcatgtcaacccatatgcatggacacagggcctcaacataatatatgtagattcacctgttggcaccggattctcttactctacaacccaagaaggttactatatagatgatgaaggtgctgtggaccagagcgttgagtttctgcggaagtggctgttggctcatcctcaatatttgaacaatatactctacattggtggcgattcttattcaggaattcctcttcctataatagtacaaaaactttttgatggtaacaatgctggagtggagccctatctaaaccttcgaggttatatacttggaaacccgaagactgatgacttcattgatgtcaactcagtgaacccatttgcacacaggttgacactcatatcagatgaactttatgaggctaccaaagaaagctgcggcggtgattacgttaatgtagacgataacaacacagcatgtgtgatagatcttgcaactattgatcagctgcttcttcaaattaatattatgcatgtcttggaacccaattgtcaagtggccttaccaaacaacagagaagaagaaggaagaagatcaatccgagaactacctgagaattttctaacagaatctactcctgcattgtggtgccggtactacaactacgtgatcttgggtgtgtgggctaatgaccacagtgtccgcaaagctcttaatgtcagcgaggtatttataattattacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
WD40

Protein Analysis

1139

Amino Acids

125.55

Weight (kDa)

5.23

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR75_1st PF23869 15 - 236 5.5e-24 WD repeat-containing protein 75 first beta-propeller
WD40_WDHD1_1st PF24817 15 - 226 4.8e-09 WDHD1 first WD40 domain
Beta-prop_WDR75_1st PF23869 268 - 337 2.7e-12 WD repeat-containing protein 75 first beta-propeller
Beta-prop_WDR75_2nd PF23769 386 - 638 1.1e-34 WD repeat-containing protein 75 second beta-propeller
WD40_Prp19 PF24814 453 - 615 1.1e-10 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 475 - 610 4.9e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 477 - 615 3.9e-08 WDR5 beta-propeller domain
Peptidase_S10 PF00450 822 - 1132 5.7e-80 Serine carboxypeptidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3407
AarI CACCTGC 1 cut(s) 647
AasI GACNNNNNNGTC 2 cut(s) 1273, 3371
Acc36I ACCTGC 2 cut(s) 647, 2490
AccB1I GGYRCC 6 cut(s) 80, 218, 719, 2347, 2762, 3321
AccB7I CCANNNNNTGG 1 cut(s) 941
AccI GTMKAC 3 cut(s) 2561, 2689, 3132
AccII CGCG 1 cut(s) 406
AccIII TCCGGA 2 cut(s) 522, 1087
AclWI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
AcoI YGGCCR 2 cut(s) 342, 582
AcuI CTGAAG 2 cut(s) 82, 1305
AcvI CACGTG 1 cut(s) 2165
AcyI GRCGYC 2 cut(s) 54, 1718
AfaI GTAC 4 cut(s) 1156, 2132, 2937, 3329
AfiI CCNNNNNNNGG 4 cut(s) 941, 1240, 1331, 1468
AflII CTTAAG 2 cut(s) 1355, 2369
AflIII ACRYGT 1 cut(s) 1859
AhlI ACTAGT 1 cut(s) 727
AjnI CCWGG 4 cut(s) 766, 2180, 2464, 2611
AjuI GAANNNNNNNTTGG 2 cut(s) 2840, 2872
AleI CACNNNNGTG 1 cut(s) 1406
Alw21I GWGCWC 1 cut(s) 1307
Alw26I GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
AlwI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
AlwNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
Ama87I CYCGRG 1 cut(s) 1773
Aor13HI TCCGGA 2 cut(s) 522, 1087
ApaI GGGCCC 1 cut(s) 556
ApeKI GCWGC 9 cut(s) 653, 904, 1110, 1607, 1610, 1646, 2616, 3108, 3181
AseI ATTAAT 2 cut(s) 831, 3195
Asp700I GAANNNNTTC 3 cut(s) 888, 2358, 2572
AspLEI GCGC 2 cut(s) 63, 2218
AsuC2I CCSGG 4 cut(s) 174, 1242, 1774, 1775
AsuHPI GGTGA 8 cut(s) 145, 346, 671, 2082, 2446, 2531, 2745, 3128
AsuII TTCGAA 1 cut(s) 329
AvaI CYCGRG 1 cut(s) 1773
AvaII GGWCC 6 cut(s) 1672, 2007, 2384, 2479, 2651, 2824
AxyI CCTNAGG 2 cut(s) 502, 1326
BaeGI GKGCMC 1 cut(s) 556
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BalI TGGCCA 1 cut(s) 584
BanI GGYRCC 6 cut(s) 80, 218, 719, 2347, 2762, 3321
BanII GRGCYC 3 cut(s) 556, 1928, 2976
BbrPI CACGTG 1 cut(s) 2165
BbsI GAAGAC 5 cut(s) 88, 266, 769, 2586, 3020
Bbv12I GWGCWC 1 cut(s) 1307
BbvCI CCTCAGC 1 cut(s) 188
BbvI GCAGC 9 cut(s) 640, 916, 1122, 1594, 1597, 1658, 2603, 3095, 3168
BceAI ACGGC 6 cut(s) 71, 143, 159, 219, 232, 2186
BcgI CGANNNNNNTGC 6 cut(s) 319, 335, 353, 369, 1356, 1390
BciT130I CCWGG 4 cut(s) 768, 2182, 2466, 2613
BclI TGATCA 1 cut(s) 3175
BcnI CCSGG 4 cut(s) 174, 1242, 1774, 1775
BcoDI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 5 cut(s) 471, 647, 728, 908, 974
BfmI CTRYAG 3 cut(s) 654, 1547, 1575
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 2 cut(s) 1355, 2369
BfuAI ACCTGC 2 cut(s) 647, 2490
BglI GCCNNNNNGGC 1 cut(s) 38
BglII AGATCT 1 cut(s) 3160
Bme1390I CCNGG 8 cut(s) 174, 768, 1242, 1774, 1775, 2182, 2466, 2613
Bme18I GGWCC 6 cut(s) 1672, 2007, 2384, 2479, 2651, 2824
BmeT110I CYCGRG 1 cut(s) 1773
BmrFI CCNGG 8 cut(s) 174, 768, 1242, 1774, 1775, 2182, 2466, 2613
BmrI ACTGGG 2 cut(s) 572, 2511
BmuI ACTGGG 2 cut(s) 572, 2511
BpiI GAAGAC 5 cut(s) 88, 266, 769, 2586, 3020
BpmI CTGGAG 5 cut(s) 568, 706, 1452, 2625, 2985
Bpu10I CCTNAGC 1 cut(s) 188
Bpu14I TTCGAA 1 cut(s) 329
BpuMI CCSGG 4 cut(s) 174, 1242, 1774, 1775
BsaAI YACGTR 3 cut(s) 1860, 2165, 3340
BsaBI GATNNNNATC 1 cut(s) 2230
BsaHI GRCGYC 2 cut(s) 54, 1718
BsaI GGTCTC 2 cut(s) 2265, 2312
BsaJI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2181, 2312, 2611
BsaWI WCCGGW 3 cut(s) 522, 1087, 2765
BsaXI ACNNNNNCTCC 4 cut(s) 879, 909, 2513, 2543
Bsc4I CCNNNNNNNGG 4 cut(s) 941, 1240, 1331, 1468
Bse118I RCCGGY 5 cut(s) 37, 77, 110, 340, 3324
Bse1I ACTGG 7 cut(s) 578, 829, 905, 1174, 2457, 2506, 2608
Bse21I CCTNAGG 2 cut(s) 502, 1326
Bse8I GATNNNNATC 1 cut(s) 2230
BseAI TCCGGA 2 cut(s) 522, 1087
BseBI CCWGG 4 cut(s) 768, 2182, 2466, 2613
BseDI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2181, 2312, 2611
BseJI GATNNNNATC 1 cut(s) 2230
BseLI CCNNNNNNNGG 4 cut(s) 941, 1240, 1331, 1468
BseMII CTCAG 9 cut(s) 42, 202, 426, 516, 1200, 1317, 1812, 3054, 3276
BseNI ACTGG 7 cut(s) 578, 829, 905, 1174, 2457, 2506, 2608
BseRI GAGGAG 2 cut(s) 56, 2521
BseSI GKGCMC 1 cut(s) 556
BseX3I CGGCCG 1 cut(s) 342
BseXI GCAGC 9 cut(s) 640, 916, 1122, 1594, 1597, 1658, 2603, 3095, 3168
BseYI CCCAGC 1 cut(s) 1742
BsgI GTGCAG 3 cut(s) 58, 1023, 1129
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 345
BshNI GGYRCC 6 cut(s) 80, 218, 719, 2347, 2762, 3321
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 1307
BsiHKCI CYCGRG 1 cut(s) 1773
BslFI GGGAC 5 cut(s) 248, 483, 1685, 2046, 2492
BslI CCNNNNNNNGG 4 cut(s) 941, 1240, 1331, 1468
BsmAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BsmBI CGTCTC 2 cut(s) 31, 94
BsmFI GGGAC 5 cut(s) 248, 483, 1685, 2046, 2492
BsmI GAATGC 1 cut(s) 1552
Bso31I GGTCTC 2 cut(s) 2265, 2312
BsoBI CYCGRG 1 cut(s) 1773
Bsp119I TTCGAA 1 cut(s) 329
Bsp120I GGGCCC 1 cut(s) 552
Bsp1286I GDGCHC 4 cut(s) 556, 1307, 1928, 2976
Bsp13I TCCGGA 2 cut(s) 522, 1087
Bsp19I CCATGG 1 cut(s) 291
BspCNI CTCAG 9 cut(s) 41, 201, 427, 515, 1201, 1318, 1813, 3053, 3277
BspEI TCCGGA 2 cut(s) 522, 1087
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 2125
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 2 cut(s) 647, 2490
BspPI GGATC 8 cut(s) 748, 779, 952, 971, 1073, 1979, 2138, 2199
BspT104I TTCGAA 1 cut(s) 329
BspT107I GGYRCC 6 cut(s) 80, 218, 719, 2347, 2762, 3321
BspTI CTTAAG 2 cut(s) 1355, 2369
BspTNI GGTCTC 2 cut(s) 2265, 2312
BsrFI RCCGGY 5 cut(s) 37, 77, 110, 340, 3324
BsrI ACTGG 7 cut(s) 578, 829, 905, 1174, 2457, 2506, 2608
BssAI RCCGGY 5 cut(s) 37, 77, 110, 340, 3324
BssECI CCNNGG 8 cut(s) 291, 396, 767, 1773, 1774, 2181, 2312, 2611
BssNI GRCGYC 2 cut(s) 54, 1718
BssT1I CCWWGG 3 cut(s) 291, 396, 2312
Bst2UI CCWGG 4 cut(s) 768, 2182, 2466, 2613
Bst4CI ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 3371
Bst6I CTCTTC 3 cut(s) 1739, 2261, 2928
BstACI GRCGYC 2 cut(s) 54, 1718
BstAFI CTTAAG 2 cut(s) 1355, 2369
BstAPI GCANNNNNTGC 2 cut(s) 1619, 1892
BstBAI YACGTR 3 cut(s) 1860, 2165, 3340
BstBI TTCGAA 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 342, 2106
BstDSI CCRYGG 1 cut(s) 291
BstEII GGTNACC 2 cut(s) 151, 698
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 2 cut(s) 63, 2218
BstMAI GTCTC 8 cut(s) 31, 94, 444, 461, 1258, 1281, 2265, 2312
BstMCI CGRYCG 1 cut(s) 345
BstNI CCWGG 4 cut(s) 768, 2182, 2466, 2613
BstNSI RCATGY 6 cut(s) 1108, 1690, 1810, 2405, 3153, 3209
BstPI GGTNACC 2 cut(s) 151, 698
BstSCI CCNGG 8 cut(s) 172, 766, 1240, 1772, 1773, 2180, 2464, 2611
BstSFI CTRYAG 3 cut(s) 654, 1547, 1575
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 9 cut(s) 640, 916, 1122, 1594, 1597, 1658, 2603, 3095, 3168
BstV2I GAAGAC 5 cut(s) 88, 266, 769, 2586, 3020
BstX2I RGATCY 5 cut(s) 740, 944, 976, 1984, 3160
BstXI CCANNNNNNTGG 1 cut(s) 2718
BstYI RGATCY 5 cut(s) 740, 944, 976, 1984, 3160
BstZI CGGCCG 1 cut(s) 342
Bsu36I CCTNAGG 2 cut(s) 502, 1326
BtgI CCRYGG 1 cut(s) 291
BtgZI GCGATG 1 cut(s) 1260
BtsI GCAGTG 1 cut(s) 1522
BtsIMutI CAGTG 7 cut(s) 585, 1131, 1314, 1522, 2601, 3048, 3376
BveI ACCTGC 2 cut(s) 647, 2490
Cac8I GCNNGC 2 cut(s) 342, 2106
CaiI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
CciI TCATGA 1 cut(s) 2125
CfoI GCGC 2 cut(s) 63, 2218
Cfr10I RCCGGY 5 cut(s) 37, 77, 110, 340, 3324
Cfr9I CCCGGG 1 cut(s) 1773
CseI GACGC 2 cut(s) 62, 1726
CsiI ACCWGGT 1 cut(s) 2464
Csp6I GTAC 4 cut(s) 1155, 2131, 2936, 3328
CspCI CAANNNNNGTGG 2 cut(s) 2082, 2117
CviQI GTAC 4 cut(s) 1155, 2131, 2936, 3328
DraI TTTAAA 1 cut(s) 2329
DrdI GACNNNNNNGTC 2 cut(s) 1273, 3371
DseDI GACNNNNNNGTC 2 cut(s) 1273, 3371
EaeI YGGCCR 2 cut(s) 342, 582
EagI CGGCCG 1 cut(s) 342
Eam1104I CTCTTC 3 cut(s) 1739, 2261, 2928
EarI CTCTTC 3 cut(s) 1739, 2261, 2928
EclXI CGGCCG 1 cut(s) 342
Eco130I CCWWGG 3 cut(s) 291, 396, 2312
Eco147I AGGCCT 1 cut(s) 2311
Eco24I GRGCYC 3 cut(s) 556, 1928, 2976
Eco31I GGTCTC 2 cut(s) 2265, 2312
Eco32I GATATC 1 cut(s) 271
Eco47I GGWCC 6 cut(s) 1672, 2007, 2384, 2479, 2651, 2824
Eco52I CGGCCG 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 82, 1305
Eco72I CACGTG 1 cut(s) 2165
Eco81I CCTNAGG 2 cut(s) 502, 1326
Eco88I CYCGRG 1 cut(s) 1773
Eco91I GGTNACC 2 cut(s) 151, 698
EcoO109I RGGNCCY 6 cut(s) 552, 553, 1672, 2479, 2609, 2728
EcoO65I GGTNACC 2 cut(s) 151, 698
EcoRI GAATTC 3 cut(s) 675, 1747, 2916
EcoRII CCWGG 4 cut(s) 766, 2180, 2464, 2611
EcoRV GATATC 1 cut(s) 271
EcoT14I CCWWGG 3 cut(s) 291, 396, 2312
EcoT22I ATGCAT 3 cut(s) 2233, 2719, 3207
EcoT38I GRGCYC 3 cut(s) 556, 1928, 2976
ErhI CCWWGG 3 cut(s) 291, 396, 2312
Esp3I CGTCTC 2 cut(s) 31, 94
FaqI GGGAC 5 cut(s) 248, 483, 1685, 2046, 2492
FauNDI CATATG 5 cut(s) 1486, 2149, 2293, 2668, 2713
FbaI TGATCA 1 cut(s) 3175
FblI GTMKAC 3 cut(s) 2561, 2689, 3132
FriOI GRGCYC 3 cut(s) 556, 1928, 2976
FspBI CTAG 5 cut(s) 471, 647, 728, 908, 974
GlaI GCGC 2 cut(s) 62, 2217
GsaI CCCAGC 1 cut(s) 1746
GsuI CTGGAG 5 cut(s) 568, 706, 1452, 2625, 2985
HaeII RGCGCY 1 cut(s) 64
HgaI GACGC 2 cut(s) 62, 1726
HhaI GCGC 2 cut(s) 63, 2218
Hin1I GRCGYC 2 cut(s) 54, 1718
Hin6I GCGC 2 cut(s) 61, 2216
HinP1I GCGC 2 cut(s) 61, 2216
HincII GTYRAC 5 cut(s) 2206, 2562, 2707, 3037, 3066
HindII GTYRAC 5 cut(s) 2206, 2562, 2707, 3037, 3066
HindIII AAGCTT 3 cut(s) 536, 1838, 2097
HphI GGTGA 8 cut(s) 145, 346, 671, 2082, 2446, 2531, 2745, 3128
Hpy99I CGWCG 2 cut(s) 56, 2566
HpyCH4III ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 3371
HpyCH4IV ACGT 5 cut(s) 24, 1859, 2164, 3123, 3339
HpySE526I ACGT 5 cut(s) 24, 1859, 2164, 3123, 3339
Hsp92I GRCGYC 2 cut(s) 54, 1718
HspAI GCGC 2 cut(s) 61, 2216
KflI GGGWCCC 1 cut(s) 1672
Kpn2I TCCGGA 2 cut(s) 522, 1087
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 3175
LmnI GCTCC 5 cut(s) 857, 1077, 1803, 2302, 2971
Lsp1109I GCAGC 9 cut(s) 640, 916, 1122, 1594, 1597, 1658, 2603, 3095, 3168
MabI ACCWGGT 1 cut(s) 2464
MaeI CTAG 5 cut(s) 471, 647, 728, 908, 974
MaeII ACGT 5 cut(s) 24, 1859, 2164, 3123, 3339
MfeI CAATTG 3 cut(s) 1722, 2537, 3220
MflI RGATCY 5 cut(s) 740, 944, 976, 1984, 3160
MhlI GDGCHC 4 cut(s) 556, 1307, 1928, 2976
MlsI TGGCCA 1 cut(s) 584
MluNI TGGCCA 1 cut(s) 584
MlyI GAGTC 5 cut(s) 446, 788, 1832, 2516, 2567
MmeI TCCRAC 3 cut(s) 72, 111, 659
Mox20I TGGCCA 1 cut(s) 584
Mph1103I ATGCAT 3 cut(s) 2233, 2719, 3207
MroI TCCGGA 2 cut(s) 522, 1087
MroNI GCCGGC 1 cut(s) 340
MroXI GAANNNNTTC 3 cut(s) 888, 2358, 2572
MscI TGGCCA 1 cut(s) 584
MslI CAYNNNNRTG 6 cut(s) 1406, 1425, 1691, 2392, 2716, 3319
Msp20I TGGCCA 1 cut(s) 584
MspA1I CMGCKG 3 cut(s) 1607, 1649, 3181
MspCI CTTAAG 2 cut(s) 1355, 2369
MspR9I CCNGG 8 cut(s) 174, 768, 1242, 1774, 1775, 2182, 2466, 2613
MunI CAATTG 3 cut(s) 1722, 2537, 3220
Mva1269I GAATGC 1 cut(s) 1552
MvaI CCWGG 4 cut(s) 768, 2182, 2466, 2613
MvnI CGCG 1 cut(s) 406
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 4 cut(s) 174, 1242, 1774, 1775
NcoI CCATGG 1 cut(s) 291
NdeI CATATG 5 cut(s) 1486, 2149, 2293, 2668, 2713
NgoMIV GCCGGC 1 cut(s) 340
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 6 cut(s) 151, 1264, 1517, 1726, 2452, 2519
NsiI ATGCAT 3 cut(s) 2233, 2719, 3207
NspI RCATGY 6 cut(s) 1108, 1690, 1810, 2405, 3153, 3209
NspV TTCGAA 1 cut(s) 329
OliI CACNNNNGTG 1 cut(s) 1406
PagI TCATGA 1 cut(s) 2125
PaqCI CACCTGC 1 cut(s) 647
PceI AGGCCT 1 cut(s) 2311
PctI GAATGC 1 cut(s) 1552
PdiI GCCGGC 1 cut(s) 342
PdmI GAANNNNTTC 3 cut(s) 888, 2358, 2572
PflFI GACNNNGTC 1 cut(s) 436
PflMI CCANNNNNTGG 1 cut(s) 941
PleI GAGTC 5 cut(s) 445, 788, 1831, 2516, 2566
PmaCI CACGTG 1 cut(s) 2165
PmlI CACGTG 1 cut(s) 2165
PpsI GAGTC 5 cut(s) 445, 788, 1831, 2516, 2566
Ppu21I YACGTR 3 cut(s) 1860, 2165, 3340
PpuMI RGGWCCY 2 cut(s) 1672, 2479
PshBI ATTAAT 2 cut(s) 831, 3195
PsiI TTATAA 1 cut(s) 3407
Psp5II RGGWCCY 2 cut(s) 1672, 2479
Psp6I CCWGG 4 cut(s) 766, 2180, 2464, 2611
PspCI CACGTG 1 cut(s) 2165
PspEI GGTNACC 2 cut(s) 151, 698
PspFI CCCAGC 1 cut(s) 1742
PspGI CCWGG 4 cut(s) 766, 2180, 2464, 2611
PspOMI GGGCCC 1 cut(s) 552
PspPPI RGGWCCY 2 cut(s) 1672, 2479
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 4 cut(s) 124, 195, 1610, 1988
PsuI RGATCY 5 cut(s) 740, 944, 976, 1984, 3160
PsyI GACNNNGTC 1 cut(s) 436
PvuII CAGCTG 3 cut(s) 1607, 1649, 3181
RsaI GTAC 4 cut(s) 1156, 2132, 2937, 3329
RsaNI GTAC 4 cut(s) 1155, 2131, 2936, 3328
RseI CAYNNNNRTG 6 cut(s) 1406, 1425, 1691, 2392, 2716, 3319
SalI GTCGAC 1 cut(s) 2560
SchI GAGTC 5 cut(s) 446, 788, 1832, 2516, 2567
ScrFI CCNGG 8 cut(s) 174, 768, 1242, 1774, 1775, 2182, 2466, 2613
SduI GDGCHC 4 cut(s) 556, 1307, 1928, 2976
SexAI ACCWGGT 1 cut(s) 2464
SfcI CTRYAG 3 cut(s) 654, 1547, 1575
SfuI TTCGAA 1 cut(s) 329
SinI GGWCC 6 cut(s) 1672, 2007, 2384, 2479, 2651, 2824
SmaI CCCGGG 1 cut(s) 1775
SmiMI CAYNNNNRTG 6 cut(s) 1406, 1425, 1691, 2392, 2716, 3319
SmlI CTYRAG 2 cut(s) 1355, 2369
SmoI CTYRAG 2 cut(s) 1355, 2369
SpeI ACTAGT 1 cut(s) 727
SseBI AGGCCT 1 cut(s) 2311
SspI AATATT 2 cut(s) 2873, 3199
SspMI CTAG 5 cut(s) 471, 647, 728, 908, 974
StuI AGGCCT 1 cut(s) 2311
StyD4I CCNGG 8 cut(s) 172, 766, 1240, 1772, 1773, 2180, 2464, 2611
StyI CCWWGG 3 cut(s) 291, 396, 2312
TaaI ACNGT 8 cut(s) 126, 223, 704, 1199, 1309, 1657, 1791, 3371
TaiI ACGT 5 cut(s) 27, 1862, 2167, 3126, 3342
TaqI TCGA 5 cut(s) 329, 442, 1125, 2561, 2989
TaqII GACCGA 1 cut(s) 2372
TatI WGTACW 2 cut(s) 2130, 2935
TauI GCSGC 4 cut(s) 15, 245, 248, 3114
TscAI CASTG 7 cut(s) 585, 1138, 1314, 1522, 2608, 3048, 3376
TseFI GTSAC 6 cut(s) 151, 1264, 1517, 1726, 2452, 2519
TseI GCWGC 9 cut(s) 653, 904, 1110, 1607, 1610, 1646, 2616, 3108, 3181
Tsp45I GTSAC 6 cut(s) 151, 1264, 1517, 1726, 2452, 2519
TspGWI ACGGA 4 cut(s) 82, 338, 1983, 1999
TspMI CCCGGG 1 cut(s) 1773
TspRI CASTG 7 cut(s) 585, 1138, 1314, 1522, 2608, 3048, 3376
Tth111I GACNNNGTC 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 941
Vha464I CTTAAG 2 cut(s) 1355, 2369
VpaK11BI GGWCC 6 cut(s) 1672, 2007, 2384, 2479, 2651, 2824
VspI ATTAAT 2 cut(s) 831, 3195
XbaI TCTAGA 1 cut(s) 973
XceI RCATGY 6 cut(s) 1108, 1690, 1810, 2405, 3153, 3209
XcmI CCANNNNNNNNNTGG 1 cut(s) 3226
XmaI CCCGGG 1 cut(s) 1773
XmiI GTMKAC 3 cut(s) 2561, 2689, 3132
XmnI GAANNNNTTC 3 cut(s) 888, 2358, 2572
XspI CTAG 5 cut(s) 471, 647, 728, 908, 974
Zsp2I ATGCAT 3 cut(s) 2233, 2719, 3207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.