Rmu_sc0004665.1_g000015

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004665.1
OG0013505
Physical Location & Seq
Reverse (-)
41392 .. 42462
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1071 bp
atgcaagtatctccatgtccaactccttcttcttccttctcatttccatcactaccccccaccaaaccccacaattacaccgccacacccttcaccttcaccttcaccacccacaaacccagactccgctccccccttatctcctccgcctccaaagatgtctggagaagaacaccaccccaccccaaaacgacgccgtcccatccctcccaaccctccccccgccaccgccgcaagctccaaggcacctccttcctggaccacagcgtcgacatggacgacctcaccgcctcaatccgcgccacccaaaacgagcacgacctcaactccctcctctctccctacaagtcccgcaacctctccatccgcttcatggtctccctcctctcccgcgagcccgactggcgccgctccctcgccatcctcgactggatcaacgacgtcgccctatactctccctccgtcttcgcctacaacgtcgtcatccgcaacgtcctccgcgccaagcagtggcaagtcgcccacggcctgttcgacgaaatgcgccacagagctctggcgccggaccgctacacttactccactctcatcaccgcgtttgggaagtccggcatgttcgacgccgcgctctcgtggctgcagaagatggaggaggaccgggtgtccggggacctggtgctgtatagcaacttgattgagctgtctaggaaactgtgtgattatagcaaagcaatttcgatattttccaggctgaagaggttggggataatgccggatttggtggcttataactcaatgatcaatgtgtttggtaaggccaagttgtttagggaggccagggttttgctgaaggagatgagggcggtgggggttttgccggatactgttagctactcgacgcttttgacaatgtacattgagaatgagaagtttgtggaggcgttggctgtgttctcggagatgaatgaggtcaagtgtccgcttgatctcacgacgtgtaatattatgattgatgtttatgggcagctgatgctgagcttttcggggaagccattcatctttttcgaatga

Protein Analysis

356

Amino Acids

40.63

Weight (kDa)

9.4

Isoelectric Point (pI)

47.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 153 - 202 1.2e-13 PPR repeat family
PPR PF01535 191 - 220 5.1e-07 PPR repeat
PPR_3 PF13812 213 - 272 2e-08 Pentatricopeptide repeat domain
PPR_long PF17177 243 - 326 2.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 255 - 287 2.3e-06 PPR repeat
PPR_2 PF13041 258 - 304 1.6e-11 PPR repeat family
PPR PF01535 261 - 290 1.2e-06 PPR repeat
PPR_3 PF13812 282 - 342 2.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 293 - 340 1.4e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 789
AasI GACNNNNNNGTC 1 cut(s) 266
AatII GACGTC 1 cut(s) 444
AccB1I GGYRCC 3 cut(s) 245, 405, 559
AccB7I CCANNNNNTGG 1 cut(s) 510
AccBSI CCGCTC 2 cut(s) 129, 411
AccI GTMKAC 1 cut(s) 270
AccII CGCG 5 cut(s) 300, 393, 501, 596, 626
AclWI GGATC 1 cut(s) 440
AcuI CTGAAG 2 cut(s) 773, 869
AcyI GRCGYC 5 cut(s) 194, 406, 441, 560, 621
AdeI CACNNNGTG 1 cut(s) 996
AfaI GTAC 1 cut(s) 914
AfiI CCNNNNNNNGG 3 cut(s) 373, 510, 600
AflIII ACRYGT 1 cut(s) 995
AhdI GACNNNNNGTC 1 cut(s) 661
AjiI CACGTC 1 cut(s) 996
AjnI CCWGG 4 cut(s) 255, 672, 746, 836
AjuI GAANNNNNNNTTGG 2 cut(s) 13, 45
AluBI AGCT 6 cut(s) 238, 554, 700, 891, 1027, 1038
AluI AGCT 6 cut(s) 238, 554, 700, 891, 1027, 1038
Alw21I GWGCWC 2 cut(s) 318, 556
Alw26I GTCTC 1 cut(s) 382
AlwI GGATC 1 cut(s) 440
AoxI GGCC 3 cut(s) 526, 816, 834
ApeKI GCWGC 2 cut(s) 637, 1024
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 6 cut(s) 302, 408, 503, 546, 562, 628
AspS9I GGNCC 4 cut(s) 259, 565, 655, 670
AsuC2I CCSGG 2 cut(s) 659, 667
AsuHPI GGTGA 5 cut(s) 85, 91, 97, 277, 583
AsuII TTCGAA 1 cut(s) 1065
AvaII GGWCC 4 cut(s) 259, 565, 655, 670
BanI GGYRCC 3 cut(s) 245, 405, 559
BanII GRGCYC 2 cut(s) 399, 556
BauI CACGAG 1 cut(s) 631
BbsI GAAGAC 1 cut(s) 457
Bbv12I GWGCWC 2 cut(s) 318, 556
BbvI GCAGC 2 cut(s) 624, 1036
BccI CCATC 5 cut(s) 55, 210, 371, 428, 640
BceAI ACGGC 2 cut(s) 181, 541
BciT130I CCWGG 4 cut(s) 257, 674, 748, 838
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 1 cut(s) 798
BcnI CCSGG 2 cut(s) 659, 667
BcoDI GTCTC 1 cut(s) 382
BfaI CTAG 1 cut(s) 705
BfmI CTRYAG 1 cut(s) 638
BfoI RGCGCY 2 cut(s) 409, 563
BfuI GTATCC 1 cut(s) 874
BglI GCCNNNNNGGC 1 cut(s) 403
BisI GCNGC 5 cut(s) 232, 409, 624, 638, 1025
BlpI GCTNAGC 1 cut(s) 1034
BlsI GCNGC 5 cut(s) 233, 410, 625, 639, 1026
Bme1390I CCNGG 6 cut(s) 257, 659, 667, 674, 748, 838
Bme18I GGWCC 4 cut(s) 259, 565, 655, 670
BmeRI GACNNNNNGTC 1 cut(s) 661
BmgBI CACGTC 1 cut(s) 996
BmgT120I GGNCC 4 cut(s) 259, 565, 655, 670
BmiI GGNNCC 4 cut(s) 247, 407, 561, 671
BmrFI CCNGG 6 cut(s) 257, 659, 667, 674, 748, 838
BmsI GCATC 1 cut(s) 1020
BpiI GAAGAC 1 cut(s) 457
BpmI CTGGAG 1 cut(s) 184
Bpu1102I GCTNAGC 1 cut(s) 1034
Bpu14I TTCGAA 1 cut(s) 1065
BpuMI CCSGG 2 cut(s) 659, 667
BsaHI GRCGYC 5 cut(s) 194, 406, 441, 560, 621
BsaI GGTCTC 1 cut(s) 382
BsaJI CCNNGG 4 cut(s) 241, 523, 666, 837
BsaXI ACNNNNNCTCC 8 cut(s) 108, 138, 311, 341, 443, 473, 563, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 373, 510, 600
Bse1I ACTGG 2 cut(s) 407, 434
BseBI CCWGG 4 cut(s) 257, 674, 748, 838
BseDI CCNNGG 4 cut(s) 241, 523, 666, 837
BseGI GGATG 4 cut(s) 202, 363, 420, 483
BseLI CCNNNNNNNGG 3 cut(s) 373, 510, 600
BseMII CTCAG 1 cut(s) 1025
BseNI ACTGG 2 cut(s) 407, 434
BseRI GAGGAG 4 cut(s) 133, 323, 374, 665
BseXI GCAGC 2 cut(s) 624, 1036
Bsh1236I CGCG 5 cut(s) 300, 393, 501, 596, 626
BshFI GGCC 3 cut(s) 528, 818, 836
BshNI GGYRCC 3 cut(s) 245, 405, 559
BsiHKAI GWGCWC 2 cut(s) 318, 556
BsiSI CCGG 6 cut(s) 563, 609, 658, 666, 773, 878
BslFI GGGAC 3 cut(s) 184, 334, 683
BslI CCNNNNNNNGG 3 cut(s) 373, 510, 600
BsmAI GTCTC 1 cut(s) 382
BsmFI GGGAC 3 cut(s) 184, 334, 683
BsnI GGCC 3 cut(s) 528, 818, 836
Bso31I GGTCTC 1 cut(s) 382
Bsp119I TTCGAA 1 cut(s) 1065
Bsp1286I GDGCHC 3 cut(s) 318, 399, 556
Bsp1407I TGTACA 1 cut(s) 912
Bsp143I GATC 3 cut(s) 432, 798, 985
Bsp1720I GCTNAGC 1 cut(s) 1034
BspANI GGCC 3 cut(s) 528, 818, 836
BspCNI CTCAG 1 cut(s) 1026
BspFNI CGCG 5 cut(s) 300, 393, 501, 596, 626
BspLI GGNNCC 4 cut(s) 247, 407, 561, 671
BspMAI CTGCAG 1 cut(s) 642
BspPI GGATC 1 cut(s) 440
BspT104I TTCGAA 1 cut(s) 1065
BspT107I GGYRCC 3 cut(s) 245, 405, 559
BspTNI GGTCTC 1 cut(s) 382
BsrBI CCGCTC 2 cut(s) 129, 411
BsrGI TGTACA 1 cut(s) 912
BsrI ACTGG 2 cut(s) 407, 434
BssECI CCNNGG 4 cut(s) 241, 523, 666, 837
BssMI GATC 3 cut(s) 432, 798, 985
BssNI GRCGYC 5 cut(s) 194, 406, 441, 560, 621
BssSI CACGAG 1 cut(s) 631
BssT1I CCWWGG 1 cut(s) 241
Bst2BI CACGAG 1 cut(s) 631
Bst2UI CCWGG 4 cut(s) 257, 674, 748, 838
Bst4CI ACNGT 2 cut(s) 714, 886
Bst6I CTCTTC 1 cut(s) 749
BstACI GRCGYC 5 cut(s) 194, 406, 441, 560, 621
BstAPI GCANNNNNTGC 1 cut(s) 1030
BstAUI TGTACA 1 cut(s) 912
BstBI TTCGAA 1 cut(s) 1065
BstC8I GCNNGC 2 cut(s) 236, 395
BstDEI CTNAG 1 cut(s) 1034
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 4 cut(s) 202, 363, 420, 483
BstFNI CGCG 5 cut(s) 300, 393, 501, 596, 626
BstH2I RGCGCY 2 cut(s) 409, 563
BstHHI GCGC 6 cut(s) 302, 408, 503, 546, 562, 628
BstKTI GATC 3 cut(s) 435, 801, 988
BstMAI GTCTC 1 cut(s) 382
BstMBI GATC 3 cut(s) 432, 798, 985
BstMWI GCNNNNNNNGC 4 cut(s) 231, 403, 634, 1030
BstNI CCWGG 4 cut(s) 257, 674, 748, 838
BstNSI RCATGY 1 cut(s) 616
BstSCI CCNGG 6 cut(s) 255, 657, 665, 672, 746, 836
BstSFI CTRYAG 1 cut(s) 638
BstUI CGCG 5 cut(s) 300, 393, 501, 596, 626
BstV1I GCAGC 2 cut(s) 624, 1036
BstV2I GAAGAC 1 cut(s) 457
BsuI GTATCC 1 cut(s) 874
BsuRI GGCC 3 cut(s) 528, 818, 836
BtgI CCRYGG 1 cut(s) 523
BtrI CACGTC 1 cut(s) 996
BtsCI GGATG 4 cut(s) 202, 363, 420, 483
BtsI GCAGTG 1 cut(s) 515
BtsIMutI CAGTG 1 cut(s) 515
Cac8I GCNNGC 2 cut(s) 236, 395
CfoI GCGC 6 cut(s) 302, 408, 503, 546, 562, 628
Cfr13I GGNCC 4 cut(s) 259, 565, 655, 670
CpoI CGGWCCG 1 cut(s) 565
CseI GACGC 4 cut(s) 202, 256, 629, 907
CsiI ACCWGGT 1 cut(s) 672
Csp6I GTAC 1 cut(s) 913
CspI CGGWCCG 1 cut(s) 565
CviAII CATG 4 cut(s) 15, 274, 373, 613
CviQI GTAC 1 cut(s) 913
DdeI CTNAG 1 cut(s) 1034
DinI GGCGCC 2 cut(s) 407, 561
DpnI GATC 3 cut(s) 434, 800, 987
DpnII GATC 3 cut(s) 432, 798, 985
DraIII CACNNNGTG 1 cut(s) 996
DrdI GACNNNNNNGTC 1 cut(s) 266
DriI GACNNNNNGTC 1 cut(s) 661
DseDI GACNNNNNNGTC 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 749
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 749
EciI GGCGGA 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 554
Eco130I CCWWGG 1 cut(s) 241
Eco24I GRGCYC 2 cut(s) 399, 556
Eco31I GGTCTC 1 cut(s) 382
Eco47I GGWCC 4 cut(s) 259, 565, 655, 670
Eco53kI GAGCTC 1 cut(s) 554
Eco57I CTGAAG 2 cut(s) 773, 869
EcoICRI GAGCTC 1 cut(s) 554
EcoO109I RGGNCCY 1 cut(s) 670
EcoRII CCWGG 4 cut(s) 255, 672, 746, 836
EcoT14I CCWWGG 1 cut(s) 241
EcoT38I GRGCYC 2 cut(s) 399, 556
EgeI GGCGCC 2 cut(s) 407, 561
EheI GGCGCC 2 cut(s) 407, 561
ErhI CCWWGG 1 cut(s) 241
FaeI CATG 4 cut(s) 18, 277, 376, 616
FaqI GGGAC 3 cut(s) 184, 334, 683
FatI CATG 4 cut(s) 14, 273, 372, 612
FauI CCCGC 3 cut(s) 230, 359, 398
FbaI TGATCA 1 cut(s) 798
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 232, 409, 624, 638, 1025
FokI GGATG 4 cut(s) 189, 350, 407, 470
FriOI GRGCYC 2 cut(s) 399, 556
Fsp4HI GCNGC 5 cut(s) 232, 409, 624, 638, 1025
FspBI CTAG 1 cut(s) 705
GlaI GCGC 6 cut(s) 301, 407, 502, 545, 561, 627
GluI GCNGC 5 cut(s) 232, 409, 624, 638, 1025
GsuI CTGGAG 1 cut(s) 184
HaeII RGCGCY 2 cut(s) 409, 563
HaeIII GGCC 3 cut(s) 528, 818, 836
HapII CCGG 6 cut(s) 563, 609, 658, 666, 773, 878
HgaI GACGC 4 cut(s) 202, 256, 629, 907
HhaI GCGC 6 cut(s) 302, 408, 503, 546, 562, 628
Hin1I GRCGYC 5 cut(s) 194, 406, 441, 560, 621
Hin1II CATG 4 cut(s) 18, 277, 376, 616
Hin6I GCGC 6 cut(s) 300, 406, 501, 544, 560, 626
HinP1I GCGC 6 cut(s) 300, 406, 501, 544, 560, 626
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 1 cut(s) 123
HpaII CCGG 6 cut(s) 563, 609, 658, 666, 773, 878
HphI GGTGA 5 cut(s) 85, 91, 97, 277, 583
Hpy166II GTNNAC 1 cut(s) 271
Hpy188I TCNGA 1 cut(s) 958
Hpy188III TCNNGA 2 cut(s) 163, 991
Hpy8I GTNNAC 1 cut(s) 271
Hpy99I CGWCG 9 cut(s) 196, 272, 443, 446, 482, 539, 623, 901, 997
HpyAV CCTTC 7 cut(s) 36, 46, 100, 106, 112, 262, 844
HpyCH4III ACNGT 2 cut(s) 714, 886
HpyCH4IV ACGT 4 cut(s) 441, 477, 492, 995
HpyCH4V TGCA 2 cut(s) 4, 640
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 403, 634, 1030
HpyF3I CTNAG 1 cut(s) 1034
HpySE526I ACGT 4 cut(s) 441, 477, 492, 995
Hsp92I GRCGYC 5 cut(s) 194, 406, 441, 560, 621
Hsp92II CATG 4 cut(s) 18, 277, 376, 616
HspAI GCGC 6 cut(s) 300, 406, 501, 544, 560, 626
KasI GGCGCC 2 cut(s) 405, 559
Ksp22I TGATCA 1 cut(s) 798
Kzo9I GATC 3 cut(s) 432, 798, 985
LmnI GCTCC 3 cut(s) 134, 243, 416
Lsp1109I GCAGC 2 cut(s) 624, 1036
LweI GCATC 1 cut(s) 1020
MabI ACCWGGT 1 cut(s) 672
MaeI CTAG 1 cut(s) 705
MaeII ACGT 4 cut(s) 441, 477, 492, 995
MalI GATC 3 cut(s) 434, 800, 987
MbiI CCGCTC 2 cut(s) 129, 411
MboI GATC 3 cut(s) 432, 798, 985
MboII GAAGA 6 cut(s) 21, 24, 180, 457, 655, 766
MhlI GDGCHC 3 cut(s) 318, 399, 556
MluCI AATT 2 cut(s) 73, 732
Mly113I GGCGCC 2 cut(s) 406, 560
MlyI GAGTC 1 cut(s) 117
MmeI TCCRAC 1 cut(s) 44
MroXI GAANNNNTTC 1 cut(s) 1052
MspA1I CMGCKG 1 cut(s) 1027
MspI CCGG 6 cut(s) 563, 609, 658, 666, 773, 878
MspR9I CCNGG 6 cut(s) 257, 659, 667, 674, 748, 838
MvaI CCWGG 4 cut(s) 257, 674, 748, 838
MvnI CGCG 5 cut(s) 300, 393, 501, 596, 626
MwoI GCNNNNNNNGC 4 cut(s) 231, 403, 634, 1030
NarI GGCGCC 2 cut(s) 406, 560
NciI CCSGG 2 cut(s) 659, 667
NdeII GATC 3 cut(s) 432, 798, 985
NlaIII CATG 4 cut(s) 18, 277, 376, 616
NlaIV GGNNCC 4 cut(s) 247, 407, 561, 671
NspI RCATGY 1 cut(s) 616
NspV TTCGAA 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 423, 474, 531
PdmI GAANNNNTTC 1 cut(s) 1052
PflFI GACNNNGTC 1 cut(s) 196
PflMI CCANNNNNTGG 1 cut(s) 510
PfoI TCCNGGA 1 cut(s) 255
PkrI GCNGC 5 cut(s) 233, 410, 625, 639, 1026
PleI GAGTC 1 cut(s) 117
PluTI GGCGCC 2 cut(s) 409, 563
PpsI GAGTC 1 cut(s) 117
PpuMI RGGWCCY 1 cut(s) 670
PsiI TTATAA 1 cut(s) 789
Psp124BI GAGCTC 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 670
Psp6I CCWGG 4 cut(s) 255, 672, 746, 836
PspGI CCWGG 4 cut(s) 255, 672, 746, 836
PspN4I GGNNCC 4 cut(s) 247, 407, 561, 671
PspPI GGNCC 4 cut(s) 259, 565, 655, 670
PspPPI RGGWCCY 1 cut(s) 670
PstI CTGCAG 1 cut(s) 642
PsyI GACNNNGTC 1 cut(s) 196
PvuII CAGCTG 1 cut(s) 1027
RsaI GTAC 1 cut(s) 914
RsaNI GTAC 1 cut(s) 913
Rsr2I CGGWCCG 1 cut(s) 565
RsrII CGGWCCG 1 cut(s) 565
SacI GAGCTC 1 cut(s) 556
SalI GTCGAC 1 cut(s) 269
SatI GCNGC 5 cut(s) 232, 409, 624, 638, 1025
Sau3AI GATC 3 cut(s) 432, 798, 985
Sau96I GGNCC 4 cut(s) 259, 565, 655, 670
SchI GAGTC 1 cut(s) 117
ScrFI CCNGG 6 cut(s) 257, 659, 667, 674, 748, 838
SduI GDGCHC 3 cut(s) 318, 399, 556
SexAI ACCWGGT 1 cut(s) 672
SfaNI GCATC 1 cut(s) 1020
SfcI CTRYAG 1 cut(s) 638
SfoI GGCGCC 2 cut(s) 407, 561
SfuI TTCGAA 1 cut(s) 1065
SinI GGWCC 4 cut(s) 259, 565, 655, 670
Sse9I AATT 2 cut(s) 73, 732
SspDI GGCGCC 2 cut(s) 405, 559
SspI AATATT 1 cut(s) 1003
SspMI CTAG 1 cut(s) 705
SstI GAGCTC 1 cut(s) 556
StyD4I CCNGG 6 cut(s) 255, 657, 665, 672, 746, 836
StyI CCWWGG 1 cut(s) 241
TaaI ACNGT 2 cut(s) 714, 886
TaiI ACGT 4 cut(s) 444, 480, 495, 998
TaqI TCGA 7 cut(s) 270, 426, 534, 618, 737, 896, 1065
TasI AATT 2 cut(s) 73, 732
TatI WGTACW 1 cut(s) 912
TauI GCSGC 3 cut(s) 234, 411, 626
TscAI CASTG 1 cut(s) 515
TseI GCWGC 2 cut(s) 637, 1024
TspDTI ATGAA 3 cut(s) 361, 977, 1045
TspGWI ACGGA 1 cut(s) 451
TspRI CASTG 1 cut(s) 515
Tth111I GACNNNGTC 1 cut(s) 196
Van91I CCANNNNNTGG 1 cut(s) 510
VpaK11BI GGWCC 4 cut(s) 259, 565, 655, 670
XceI RCATGY 1 cut(s) 616
XcmI CCANNNNNNNNNTGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 1 cut(s) 705
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.