Rmu_sc0005514.1_g000021

Glucosidase 2 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005514.1
OG0008797
Physical Location & Seq
Forward (+)
90008 .. 93926
3919 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
atggagacgagtttcatatggtcagtctccgcctgtttctttctggtctcgtccttctcctttgttcgctcattctccaatccatctcttcttggagttcatccactcgatgaaaagtactttgctccggaggtgatcaagtgcaaggacgattccaagtcgttcaccagagaccgtctcaatgataatttctgtgactgtgttgatgggactgacgagcctgggacttctgcttgtcctgcaggcaaattttattgtcgtaatattggaagtacaccacagttcatattttcttctcgagttaatgatcgtatttgtgattgctgcgatggaagtgatgagaatgatggtagtctcaattgtccaaatacatgcatcatgggtgggaatgttgagtacaaaactgaccaccgcttctctagagttcaggatcgagggtatgttgacaaaaagcaaaccgaaaatagagtcagtctggatgacttcgttcagaagcttaaaggtttgaagataataataatcgtacaattagtgattgctagtgtttttgtggtttgccggatattccatcgtcatgccagatcaaaacgaagacgctatcaaaatggtatttgcagtattttttctggtcctataccaatatttggccgacgtgcttcctcaggatctgtgtatgggtttcaagaaaacgaagtgctggaggatttattgaatcgaattcctccgataactattccgatttcgactcctgtgagcagaacgatgaagattcggagtgtgattgatgtgagtgaggagtacgagagtattgtcaagagctttgttgtgggtatgggatcggcgggcggagttacggaggttgtggcgatgcacaagaaattgatttatggactgatcgcgctgtacttgaatattaatagcctgaaaaatgaaggagaacaacgacaccggcggcgacggcgggtatgtgaggacggaccaaatagatctgaagagcttggacgagcagcttcagaagcatctgagcagggcatggactatggagaagaacaagaacagagaccagcaggaacaacaaatctgaatattaggttgttcaaagaaatagtcataccatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
PRKCSH-like

Protein Analysis

372

Amino Acids

41.83

Weight (kDa)

7.88

Isoelectric Point (pI)

49.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRKCSH-like PF12999 26 - 131 5.8e-30 Glucosidase II beta subunit-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 644
AccII CGCG 1 cut(s) 899
AccIII TCCGGA 1 cut(s) 127
AciI CCGC 6 cut(s) 30, 412, 842, 846, 952, 961
AclWI GGATC 3 cut(s) 438, 673, 844
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 2 cut(s) 248, 717
AcuI CTGAAG 2 cut(s) 996, 1011
AfaI GTAC 6 cut(s) 119, 274, 398, 525, 800, 905
AfiI CCNNNNNNNGG 1 cut(s) 644
AgsI TTSAA 5 cut(s) 508, 683, 712, 910, 1099
AjiI CACGTC 1 cut(s) 653
AjnI CCWGG 1 cut(s) 220
AluBI AGCT 4 cut(s) 496, 819, 997, 1010
AluI AGCT 4 cut(s) 496, 819, 997, 1010
Alw26I GTCTC 6 cut(s) 31, 52, 165, 182, 359, 1054
AlwI GGATC 3 cut(s) 438, 673, 844
AlwNI CAGNNNCTG 1 cut(s) 668
Ama87I CYCGRG 1 cut(s) 297
Aor13HI TCCGGA 1 cut(s) 127
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 2 cut(s) 324, 1007
ApoI RAATTY 2 cut(s) 248, 717
ArsI GACNNNNNNTTYG 3 cut(s) 453, 485, 1092
AseI ATTAAT 1 cut(s) 915
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 2 cut(s) 629, 977
AsuHPI GGTGA 2 cut(s) 145, 157
AvaI CYCGRG 1 cut(s) 297
AvaII GGWCC 2 cut(s) 629, 977
AxyI CCTNAGG 1 cut(s) 661
BbsI GAAGAC 1 cut(s) 598
BbvI GCAGC 2 cut(s) 311, 1019
BccI CCATC 5 cut(s) 91, 200, 323, 341, 576
BceAI ACGGC 1 cut(s) 974
BciT130I CCWGG 1 cut(s) 222
BclI TGATCA 1 cut(s) 135
BcoDI GTCTC 6 cut(s) 31, 52, 165, 182, 359, 1054
BfaI CTAG 2 cut(s) 420, 540
BfmI CTRYAG 1 cut(s) 240
BglII AGATCT 1 cut(s) 986
BisI GCNGC 3 cut(s) 325, 953, 1008
BlsI GCNGC 3 cut(s) 326, 954, 1009
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 2 cut(s) 629, 977
BmeT110I CYCGRG 1 cut(s) 297
BmgBI CACGTC 1 cut(s) 653
BmgT120I GGNCC 2 cut(s) 629, 977
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 3 cut(s) 384, 858, 1028
BpiI GAAGAC 1 cut(s) 598
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpmI CTGGAG 1 cut(s) 719
BsaI GGTCTC 3 cut(s) 52, 165, 1054
BsaJI CCNNGG 1 cut(s) 221
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 1 cut(s) 644
Bse118I RCCGGY 1 cut(s) 948
Bse21I CCTNAGG 1 cut(s) 661
BseAI TCCGGA 1 cut(s) 127
BseBI CCWGG 1 cut(s) 222
BseDI CCNNGG 1 cut(s) 221
BseGI GGATG 2 cut(s) 100, 484
BseLI CCNNNNNNNGG 1 cut(s) 644
BseMII CTCAG 2 cut(s) 675, 1014
BseRI GAGGAG 1 cut(s) 809
BseXI GCAGC 2 cut(s) 311, 1019
Bsh1236I CGCG 1 cut(s) 899
BshFI GGCC 1 cut(s) 648
BsiHKCI CYCGRG 1 cut(s) 297
BsiSI CCGG 3 cut(s) 128, 559, 949
BslFI GGGAC 2 cut(s) 223, 238
BslI CCNNNNNNNGG 1 cut(s) 644
BsmAI GTCTC 6 cut(s) 31, 52, 165, 182, 359, 1054
BsmBI CGTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 223, 238
BsnI GGCC 1 cut(s) 648
Bso31I GGTCTC 3 cut(s) 52, 165, 1054
BsoBI CYCGRG 1 cut(s) 297
Bsp13I TCCGGA 1 cut(s) 127
Bsp143I GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
BspACI CCGC 6 cut(s) 30, 412, 842, 846, 952, 961
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 2 cut(s) 674, 1015
BspEI TCCGGA 1 cut(s) 127
BspFNI CGCG 1 cut(s) 899
BspMAI CTGCAG 1 cut(s) 244
BspPI GGATC 3 cut(s) 438, 673, 844
BspQI GCTCTTC 1 cut(s) 987
BspTNI GGTCTC 3 cut(s) 52, 165, 1054
BsrFI RCCGGY 1 cut(s) 948
BssAI RCCGGY 1 cut(s) 948
BssECI CCNNGG 1 cut(s) 221
BssMI GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 3 cut(s) 176, 200, 282
Bst6I CTCTTC 2 cut(s) 93, 987
BstC8I GCNNGC 2 cut(s) 244, 844
BstDEI CTNAG 2 cut(s) 661, 1023
BstF5I GGATG 2 cut(s) 100, 484
BstFNI CGCG 1 cut(s) 899
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 8 cut(s) 138, 310, 433, 584, 668, 839, 897, 989
BstMAI GTCTC 6 cut(s) 31, 52, 165, 182, 359, 1054
BstMBI GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
BstMWI GCNNNNNNNGC 3 cut(s) 239, 958, 1016
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 1 cut(s) 899
BstV1I GCAGC 2 cut(s) 311, 1019
BstV2I GAAGAC 1 cut(s) 598
BstX2I RGATCY 2 cut(s) 665, 986
BstYI RGATCY 2 cut(s) 665, 986
Bsu36I CCTNAGG 1 cut(s) 661
BsuRI GGCC 1 cut(s) 648
BtgZI GCGATG 2 cut(s) 342, 881
BtrI CACGTC 1 cut(s) 653
BtsCI GGATG 2 cut(s) 100, 484
Cac8I GCNNGC 2 cut(s) 244, 844
CaiI CAGNNNCTG 1 cut(s) 668
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 1 cut(s) 948
Cfr13I GGNCC 2 cut(s) 629, 977
CseI GACGC 1 cut(s) 603
Csp6I GTAC 6 cut(s) 118, 273, 397, 524, 799, 904
CviAII CATG 5 cut(s) 372, 379, 575, 1033, 1116
CviJI RGCY 7 cut(s) 220, 496, 648, 819, 921, 997, 1010
CviKI_1 RGCY 7 cut(s) 220, 496, 648, 819, 921, 997, 1010
CviQI GTAC 6 cut(s) 118, 273, 397, 524, 799, 904
DdeI CTNAG 2 cut(s) 661, 1023
DpnI GATC 8 cut(s) 137, 309, 432, 583, 667, 838, 896, 988
DpnII GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
EaeI YGGCCR 1 cut(s) 646
Eam1104I CTCTTC 2 cut(s) 93, 987
EarI CTCTTC 2 cut(s) 93, 987
EciI GGCGGA 2 cut(s) 19, 861
Eco31I GGTCTC 3 cut(s) 52, 165, 1054
Eco47I GGWCC 2 cut(s) 629, 977
Eco57I CTGAAG 2 cut(s) 996, 1011
Eco81I CCTNAGG 1 cut(s) 661
Eco88I CYCGRG 1 cut(s) 297
EcoRI GAATTC 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 220
EcoT22I ATGCAT 1 cut(s) 377
Esp3I CGTCTC 1 cut(s) 182
FaeI CATG 5 cut(s) 375, 382, 578, 1036, 1119
FaqI GGGAC 2 cut(s) 223, 238
FatI CATG 5 cut(s) 371, 378, 574, 1032, 1115
FauI CCCGC 2 cut(s) 835, 954
FauNDI CATATG 1 cut(s) 17
FbaI TGATCA 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 325, 953, 1008
FokI GGATG 2 cut(s) 87, 491
Fsp4HI GCNGC 3 cut(s) 325, 953, 1008
FspBI CTAG 2 cut(s) 420, 540
GlaI GCGC 1 cut(s) 900
GluI GCNGC 3 cut(s) 325, 953, 1008
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 1 cut(s) 648
HapII CCGG 3 cut(s) 128, 559, 949
HgaI GACGC 1 cut(s) 603
HhaI GCGC 1 cut(s) 901
Hin1II CATG 5 cut(s) 375, 382, 578, 1036, 1119
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HincII GTYRAC 1 cut(s) 445
HindII GTYRAC 1 cut(s) 445
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 5 cut(s) 152, 468, 712, 745, 769
HpaII CCGG 3 cut(s) 128, 559, 949
HphI GGTGA 2 cut(s) 145, 157
Hpy166II GTNNAC 3 cut(s) 165, 275, 445
Hpy188I TCNGA 8 cut(s) 492, 726, 738, 774, 991, 1015, 1024, 1083
Hpy188III TCNNGA 8 cut(s) 128, 297, 420, 428, 476, 663, 683, 814
Hpy8I GTNNAC 3 cut(s) 165, 275, 445
Hpy99I CGWCG 2 cut(s) 654, 960
HpyAV CCTTC 2 cut(s) 64, 926
HpyCH4III ACNGT 3 cut(s) 176, 200, 282
HpyCH4IV ACGT 1 cut(s) 652
HpyCH4V TGCA 5 cut(s) 144, 242, 375, 615, 871
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 958, 1016
HpyF3I CTNAG 2 cut(s) 661, 1023
HpySE526I ACGT 1 cut(s) 652
Hsp92II CATG 5 cut(s) 375, 382, 578, 1036, 1119
HspAI GCGC 1 cut(s) 899
Kpn2I TCCGGA 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
LguI GCTCTTC 1 cut(s) 987
LmnI GCTCC 1 cut(s) 130
Lsp1109I GCAGC 2 cut(s) 311, 1019
LweI GCATC 3 cut(s) 384, 858, 1028
MaeI CTAG 2 cut(s) 420, 540
MaeII ACGT 1 cut(s) 652
MaeIII GTNAC 2 cut(s) 194, 850
MalI GATC 8 cut(s) 137, 309, 432, 583, 667, 838, 896, 988
MboI GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
MboII GAAGA 7 cut(s) 80, 285, 520, 603, 778, 1004, 1058
MfeI CAATTG 1 cut(s) 358
MflI RGATCY 2 cut(s) 665, 986
MluCI AATT 6 cut(s) 187, 248, 358, 527, 717, 878
MlyI GAGTC 2 cut(s) 477, 739
MnlI CCTC 8 cut(s) 124, 428, 670, 694, 732, 787, 850, 964
Mph1103I ATGCAT 1 cut(s) 377
MroI TCCGGA 1 cut(s) 127
MseI TTAA 3 cut(s) 303, 498, 915
MslI CAYNNNNRTG 1 cut(s) 573
MspI CCGG 3 cut(s) 128, 559, 949
MspR9I CCNGG 1 cut(s) 222
MunI CAATTG 1 cut(s) 358
MvaI CCWGG 1 cut(s) 222
MvnI CGCG 1 cut(s) 899
MwoI GCNNNNNNNGC 3 cut(s) 239, 958, 1016
NdeI CATATG 1 cut(s) 17
NdeII GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
NlaIII CATG 5 cut(s) 375, 382, 578, 1036, 1119
NmuCI GTSAC 1 cut(s) 194
NsiI ATGCAT 1 cut(s) 377
NspI RCATGY 1 cut(s) 375
PaeR7I CTCGAG 1 cut(s) 297
PciSI GCTCTTC 1 cut(s) 987
PfeI GAWTC 3 cut(s) 152, 712, 769
PflMI CCANNNNNTGG 1 cut(s) 644
PkrI GCNGC 3 cut(s) 326, 954, 1009
PleI GAGTC 2 cut(s) 476, 739
PpsI GAGTC 2 cut(s) 476, 739
PshBI ATTAAT 1 cut(s) 915
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspPI GGNCC 2 cut(s) 629, 977
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 1 cut(s) 668
PsuI RGATCY 2 cut(s) 665, 986
RsaI GTAC 6 cut(s) 119, 274, 398, 525, 800, 905
RsaNI GTAC 6 cut(s) 118, 273, 397, 524, 799, 904
RseI CAYNNNNRTG 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 987
SaqAI TTAA 3 cut(s) 303, 498, 915
SatI GCNGC 3 cut(s) 325, 953, 1008
Sau3AI GATC 8 cut(s) 135, 307, 430, 581, 665, 836, 894, 986
Sau96I GGNCC 2 cut(s) 629, 977
SbfI CCTGCAGG 1 cut(s) 244
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 2 cut(s) 477, 739
ScrFI CCNGG 1 cut(s) 222
SdaI CCTGCAGG 1 cut(s) 244
SetI ASST 9 cut(s) 135, 498, 505, 655, 821, 861, 999, 1012, 1094
SfaNI GCATC 3 cut(s) 384, 858, 1028
SfcI CTRYAG 1 cut(s) 240
Sfr274I CTCGAG 1 cut(s) 297
SgrAI CRCCGGYG 1 cut(s) 948
SinI GGWCC 2 cut(s) 629, 977
SlaI CTCGAG 1 cut(s) 297
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 1 cut(s) 297
SmoI CTYRAG 1 cut(s) 297
Sse8387I CCTGCAGG 1 cut(s) 244
Sse9I AATT 6 cut(s) 187, 248, 358, 527, 717, 878
SsiI CCGC 6 cut(s) 30, 412, 842, 846, 952, 961
SspI AATATT 4 cut(s) 265, 642, 913, 1087
SspMI CTAG 2 cut(s) 420, 540
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 3 cut(s) 176, 200, 282
TaiI ACGT 1 cut(s) 655
TaqI TCGA 5 cut(s) 108, 298, 433, 715, 743
TasI AATT 6 cut(s) 187, 248, 358, 527, 717, 878
TatI WGTACW 4 cut(s) 117, 272, 396, 903
TauI GCSGC 1 cut(s) 955
TfiI GAWTC 3 cut(s) 152, 712, 769
Tru1I TTAA 3 cut(s) 303, 498, 915
Tru9I TTAA 3 cut(s) 303, 498, 915
TseFI GTSAC 1 cut(s) 194
TseI GCWGC 2 cut(s) 324, 1007
Tsp45I GTSAC 1 cut(s) 194
TspDTI ATGAA 6 cut(s) 4, 89, 126, 274, 779, 945
TspGWI ACGGA 2 cut(s) 869, 990
Van91I CCANNNNNTGG 1 cut(s) 644
VpaK11BI GGWCC 2 cut(s) 629, 977
VspI ATTAAT 1 cut(s) 915
XapI RAATTY 2 cut(s) 248, 717
XbaI TCTAGA 1 cut(s) 419
XceI RCATGY 1 cut(s) 375
XhoI CTCGAG 1 cut(s) 297
XspI CTAG 2 cut(s) 420, 540
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.