Rmu_sc0005528.1_g000005

leucine-rich repeat extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005528.1
OG0013534
Physical Location & Seq
Forward (+)
33462 .. 34664
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005528.1_g000005.1.cds

Sequence Viewer

Length: 1203 bp
atggaagtgaatgaaaacaaagccatttcatttattctctttgttgtgtatgtctccagtttgttcaaccatctcaatctggcttctgttgctgcaaaacacagcagccgcaatagtgtacatgcttctcatcaccgtggccatcaccgtggccgccaccatagtaaccattctgattttgctgcagcatcagatgatcattccaatgcaagattggaccgagcttttcttgccctccaagcatggaaacgagtgatttactccgacccaaataactttaccaccaattggaaaggtccttcagtttgcacttacaagggtgtgtactgtgcacctgctcctgatgagcccaaaacccaagttgttgctggcattgaccttaataaaggtgacatagccggattcctcccagacgaattaggcctcttgtcggaccttgcacttatccatctgaacagcaaccgcttttgcggcatcctccctcagagtttggccaacctcacactccttaatgagcttgatctcagtaacaacagatttgtgggtccttttcctactgtagtcctctctcttcctagtctaaaatatcttgatattcgctacaatgagtttgaagggacaatacctccacaactcttcagcaacatagtacaactagatgcaatatttgtaaataacaaccgctttaccagtataaatattgcagcttctaatttatcatcatcagccagtgtggtggtgtttgctaacaataaattgggcggatgcctttctccgagcatagcaaactttgcagatacattggaggagcttttgctaatcaacacaagcttgtcggggtgtcttccacaacaagttgggtttctgtacaaattgagggtgttggatgtgagcaataacaacctggttggtcctataccttacagccttgcaggtctagctcatttggagcaactaaatttggccaacaatatgatgactgggattgtgccagctggagtttgtagtttgccaaatttggcaaattttacaatctcttataattatttctgcgaggaagacgggatttgtcagaatttgacaacatccaaaggaggcaagactgcatttgatgatcgccgtaactgtgtgcctgagaggccataccagaggtcccagaaggaatgtaatgctgctcttgagcaccctgtttactgcttttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8

Protein Analysis

400

Amino Acids

43.9

Weight (kDa)

6.38

Isoelectric Point (pI)

33.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013534)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28380
fragaria_vesca FvH4_6g26510
malus_domestica MD17G1236200.v1.1
prunus_persica Prupe.3G139500_v2.0.a1
pyrus_communis pycom17g23940
rosa_chinensis RchiOBHm_Chr2g0127651
rosa_laevigata RLG00000018945
rosa_multiflora Rmu_co8443749.1_g000001 Rmu_sc0005528.1_g000004 Rmu_sc0005528.1_g000005 Rmu_ssc0000200.1_g000005
rosa_roxburghii Rroxscaffold_2G00116700
rosa_rugosa Rorug02G0272100
rosa_samantha Rh2AG324100 Rh2BG333200 Rh2CG311600 Rh2DG351500
rosa_wichuraiana Rw2G026310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1041
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 2 cut(s) 343, 923
AccB7I CCANNNNNTGG 1 cut(s) 288
AciI CCGC 6 cut(s) 109, 154, 463, 471, 682, 762
AcoI YGGCCR 4 cut(s) 139, 151, 492, 963
AcsI RAATTY 4 cut(s) 958, 1015, 1024, 1075
AcuI CTGAAG 2 cut(s) 285, 622
AfaI GTAC 4 cut(s) 120, 326, 651, 869
AfiI CCNNNNNNNGG 2 cut(s) 288, 430
AgsI TTSAA 2 cut(s) 67, 614
AjnI CCWGG 1 cut(s) 903
AluBI AGCT 7 cut(s) 224, 517, 707, 811, 831, 941, 995
AluI AGCT 7 cut(s) 224, 517, 707, 811, 831, 941, 995
Alw21I GWGCWC 2 cut(s) 334, 1185
Alw26I GTCTC 1 cut(s) 58
Alw44I GTGCAC 1 cut(s) 330
AoxI GGCC 6 cut(s) 139, 151, 421, 492, 963, 1139
ApaLI GTGCAC 1 cut(s) 330
ApeKI GCWGC 6 cut(s) 92, 105, 182, 185, 704, 1172
ApoI RAATTY 4 cut(s) 958, 1015, 1024, 1075
AspS9I GGNCC 6 cut(s) 217, 296, 433, 545, 911, 1152
AsuHPI GGTGA 3 cut(s) 125, 137, 401
AvaII GGWCC 6 cut(s) 217, 296, 433, 545, 911, 1152
BaeGI GKGCMC 1 cut(s) 334
BalI TGGCCA 3 cut(s) 141, 494, 965
BanII GRGCYC 1 cut(s) 351
BarI GAAGNNNNNNTAC 2 cut(s) 606, 638
BbsI GAAGAC 2 cut(s) 836, 1065
Bbv12I GWGCWC 2 cut(s) 334, 1185
BbvI GCAGC 6 cut(s) 79, 117, 169, 197, 716, 1159
BccI CCATC 3 cut(s) 78, 150, 456
BceAI ACGGC 1 cut(s) 1104
BciT130I CCWGG 1 cut(s) 905
BclI TGATCA 1 cut(s) 196
BcoDI GTCTC 1 cut(s) 58
BfaI CTAG 3 cut(s) 576, 656, 938
BfmI CTRYAG 2 cut(s) 183, 558
BfuAI ACCTGC 2 cut(s) 343, 923
BglI GCCNNNNNGGC 1 cut(s) 1138
BisI GCNGC 9 cut(s) 93, 106, 109, 154, 183, 186, 472, 705, 1173
BlsI GCNGC 9 cut(s) 94, 107, 110, 155, 184, 187, 473, 706, 1174
Bme1390I CCNGG 1 cut(s) 905
Bme18I GGWCC 6 cut(s) 217, 296, 433, 545, 911, 1152
BmgT120I GGNCC 6 cut(s) 217, 296, 433, 545, 911, 1152
BmiI GGNNCC 2 cut(s) 546, 1154
BmrFI CCNGG 1 cut(s) 905
BmrI ACTGGG 1 cut(s) 990
BmsI GCATC 4 cut(s) 197, 483, 649, 755
BmuI ACTGGG 1 cut(s) 990
BpiI GAAGAC 2 cut(s) 836, 1065
BpmI CTGGAG 2 cut(s) 40, 1017
BpuEI CTTGAG 1 cut(s) 1199
BsaJI CCNNGG 2 cut(s) 136, 148
BsaXI ACNNNNNCTCC 2 cut(s) 610, 640
Bsc4I CCNNNNNNNGG 2 cut(s) 288, 430
Bse1I ACTGG 4 cut(s) 57, 690, 729, 985
BseBI CCWGG 1 cut(s) 905
BseDI CCNNGG 2 cut(s) 136, 148
BseGI GGATG 4 cut(s) 474, 770, 892, 1085
BseLI CCNNNNNNNGG 2 cut(s) 288, 430
BseMII CTCAG 3 cut(s) 497, 538, 1125
BseNI ACTGG 4 cut(s) 57, 690, 729, 985
BseRI GAGGAG 1 cut(s) 821
BseSI GKGCMC 1 cut(s) 334
BseXI GCAGC 6 cut(s) 79, 117, 169, 197, 716, 1159
BshFI GGCC 6 cut(s) 141, 153, 423, 494, 965, 1141
BsiHKAI GWGCWC 2 cut(s) 334, 1185
BsiSI CCGG 1 cut(s) 399
BslFI GGGAC 2 cut(s) 631, 1138
BslI CCNNNNNNNGG 2 cut(s) 288, 430
BsmAI GTCTC 1 cut(s) 58
BsmFI GGGAC 2 cut(s) 631, 1138
BsnI GGCC 6 cut(s) 141, 153, 423, 494, 965, 1141
Bsp1286I GDGCHC 3 cut(s) 334, 351, 1185
Bsp1407I TGTACA 2 cut(s) 118, 867
Bsp143I GATC 3 cut(s) 196, 520, 1114
BspACI CCGC 6 cut(s) 109, 154, 463, 471, 682, 762
BspANI GGCC 6 cut(s) 141, 153, 423, 494, 965, 1141
BspCNI CTCAG 3 cut(s) 496, 537, 1126
BspLI GGNNCC 2 cut(s) 546, 1154
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 2 cut(s) 343, 923
BsrGI TGTACA 2 cut(s) 118, 867
BsrI ACTGG 4 cut(s) 57, 690, 729, 985
BssECI CCNNGG 2 cut(s) 136, 148
BssMI GATC 3 cut(s) 196, 520, 1114
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 5 cut(s) 137, 149, 329, 559, 1127
Bst6I CTCTTC 2 cut(s) 576, 641
BstAPI GCANNNNNTGC 1 cut(s) 791
BstAUI TGTACA 2 cut(s) 118, 867
BstC8I GCNNGC 2 cut(s) 370, 993
BstDEI CTNAG 3 cut(s) 483, 524, 1134
BstDSI CCRYGG 2 cut(s) 136, 148
BstF5I GGATG 4 cut(s) 474, 770, 892, 1085
BstKTI GATC 3 cut(s) 199, 523, 1117
BstMAI GTCTC 1 cut(s) 58
BstMBI GATC 3 cut(s) 196, 520, 1114
BstMWI GCNNNNNNNGC 7 cut(s) 89, 230, 239, 471, 791, 938, 1138
BstNI CCWGG 1 cut(s) 905
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 903
BstSFI CTRYAG 2 cut(s) 183, 558
BstSLI GKGCMC 1 cut(s) 334
BstV1I GCAGC 6 cut(s) 79, 117, 169, 197, 716, 1159
BstV2I GAAGAC 2 cut(s) 836, 1065
BstXI CCANNNNNNTGG 2 cut(s) 149, 736
BsuRI GGCC 6 cut(s) 141, 153, 423, 494, 965, 1141
BtgI CCRYGG 2 cut(s) 136, 148
BtsCI GGATG 4 cut(s) 474, 770, 892, 1085
BtsIMutI CAGTG 1 cut(s) 736
BveI ACCTGC 2 cut(s) 343, 923
Cac8I GCNNGC 2 cut(s) 370, 993
Cfr13I GGNCC 6 cut(s) 217, 296, 433, 545, 911, 1152
CsiI ACCWGGT 1 cut(s) 903
Csp6I GTAC 4 cut(s) 119, 325, 650, 868
CviAII CATG 2 cut(s) 122, 243
CviQI GTAC 4 cut(s) 119, 325, 650, 868
DdeI CTNAG 3 cut(s) 483, 524, 1134
DpnI GATC 3 cut(s) 198, 522, 1116
DpnII GATC 3 cut(s) 196, 520, 1114
EaeI YGGCCR 4 cut(s) 139, 151, 492, 963
Eam1104I CTCTTC 2 cut(s) 576, 641
EarI CTCTTC 2 cut(s) 576, 641
EciI GGCGGA 1 cut(s) 777
Eco147I AGGCCT 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 351
Eco47I GGWCC 6 cut(s) 217, 296, 433, 545, 911, 1152
Eco57I CTGAAG 2 cut(s) 285, 622
EcoO109I RGGNCCY 3 cut(s) 296, 545, 1152
EcoRII CCWGG 1 cut(s) 903
EcoT38I GRGCYC 1 cut(s) 351
FaeI CATG 2 cut(s) 125, 246
FaqI GGGAC 2 cut(s) 631, 1138
FatI CATG 2 cut(s) 121, 242
FbaI TGATCA 1 cut(s) 196
Fnu4HI GCNGC 9 cut(s) 93, 106, 109, 154, 183, 186, 472, 705, 1173
FokI GGATG 4 cut(s) 461, 777, 899, 1072
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 9 cut(s) 93, 106, 109, 154, 183, 186, 472, 705, 1173
FspBI CTAG 3 cut(s) 576, 656, 938
GluI GCNGC 9 cut(s) 93, 106, 109, 154, 183, 186, 472, 705, 1173
GsuI CTGGAG 2 cut(s) 40, 1017
HaeIII GGCC 6 cut(s) 141, 153, 423, 494, 965, 1141
HapII CCGG 1 cut(s) 399
Hin1II CATG 2 cut(s) 125, 246
HindIII AAGCTT 1 cut(s) 829
HinfI GANTC 1 cut(s) 402
HpaII CCGG 1 cut(s) 399
HphI GGTGA 3 cut(s) 125, 137, 401
Hpy166II GTNNAC 4 cut(s) 119, 325, 332, 1192
Hpy188I TCNGA 8 cut(s) 175, 193, 265, 433, 453, 486, 777, 1074
Hpy188III TCNNGA 3 cut(s) 341, 590, 1178
Hpy8I GTNNAC 4 cut(s) 119, 325, 332, 1192
HpyAV CCTTC 3 cut(s) 309, 608, 1153
HpyCH4III ACNGT 5 cut(s) 137, 149, 329, 559, 1127
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 230, 239, 471, 791, 938, 1138
HpyF3I CTNAG 3 cut(s) 483, 524, 1134
Hsp92II CATG 2 cut(s) 125, 246
Ksp22I TGATCA 1 cut(s) 196
Kzo9I GATC 3 cut(s) 196, 520, 1114
LmnI GCTCC 3 cut(s) 343, 808, 949
Lsp1109I GCAGC 6 cut(s) 79, 117, 169, 197, 716, 1159
LweI GCATC 4 cut(s) 197, 483, 649, 755
MabI ACCWGGT 1 cut(s) 903
MaeI CTAG 3 cut(s) 576, 656, 938
MaeIII GTNAC 4 cut(s) 164, 389, 527, 1121
MalI GATC 3 cut(s) 198, 522, 1116
MboI GATC 3 cut(s) 196, 520, 1114
MboII GAAGA 4 cut(s) 563, 628, 836, 1070
MfeI CAATTG 1 cut(s) 286
MhlI GDGCHC 3 cut(s) 334, 351, 1185
MlsI TGGCCA 3 cut(s) 141, 494, 965
MluNI TGGCCA 3 cut(s) 141, 494, 965
MmeI TCCRAC 3 cut(s) 288, 411, 864
Mox20I TGGCCA 3 cut(s) 141, 494, 965
MscI TGGCCA 3 cut(s) 141, 494, 965
MseI TTAA 3 cut(s) 381, 510, 1201
MslI CAYNNNNRTG 3 cut(s) 135, 147, 204
Msp20I TGGCCA 3 cut(s) 141, 494, 965
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 1 cut(s) 399
MspR9I CCNGG 1 cut(s) 905
MunI CAATTG 1 cut(s) 286
MvaI CCWGG 1 cut(s) 905
MwoI GCNNNNNNNGC 7 cut(s) 89, 230, 239, 471, 791, 938, 1138
NdeII GATC 3 cut(s) 196, 520, 1114
NlaIII CATG 2 cut(s) 125, 246
NlaIV GGNNCC 2 cut(s) 546, 1154
NmuCI GTSAC 1 cut(s) 389
NspI RCATGY 1 cut(s) 125
PaqCI CACCTGC 1 cut(s) 343
PceI AGGCCT 1 cut(s) 423
PfeI GAWTC 1 cut(s) 402
PflMI CCANNNNNTGG 1 cut(s) 288
PkrI GCNGC 9 cut(s) 94, 107, 110, 155, 184, 187, 473, 706, 1174
PpuMI RGGWCCY 3 cut(s) 296, 545, 1152
PsiI TTATAA 1 cut(s) 1041
Psp5II RGGWCCY 3 cut(s) 296, 545, 1152
Psp6I CCWGG 1 cut(s) 903
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 2 cut(s) 546, 1154
PspPI GGNCC 6 cut(s) 217, 296, 433, 545, 911, 1152
PspPPI RGGWCCY 3 cut(s) 296, 545, 1152
PstI CTGCAG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 4 cut(s) 120, 326, 651, 869
RsaNI GTAC 4 cut(s) 119, 325, 650, 868
RseI CAYNNNNRTG 3 cut(s) 135, 147, 204
SaqAI TTAA 3 cut(s) 381, 510, 1201
SatI GCNGC 9 cut(s) 93, 106, 109, 154, 183, 186, 472, 705, 1173
Sau3AI GATC 3 cut(s) 196, 520, 1114
Sau96I GGNCC 6 cut(s) 217, 296, 433, 545, 911, 1152
ScrFI CCNGG 1 cut(s) 905
SduI GDGCHC 3 cut(s) 334, 351, 1185
SexAI ACCWGGT 1 cut(s) 903
SfaNI GCATC 4 cut(s) 197, 483, 649, 755
SfcI CTRYAG 2 cut(s) 183, 558
SinI GGWCC 6 cut(s) 217, 296, 433, 545, 911, 1152
SmiMI CAYNNNNRTG 3 cut(s) 135, 147, 204
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
SseBI AGGCCT 1 cut(s) 423
SsiI CCGC 6 cut(s) 109, 154, 463, 471, 682, 762
SspI AATATT 2 cut(s) 666, 700
SspMI CTAG 3 cut(s) 576, 656, 938
StuI AGGCCT 1 cut(s) 423
StyD4I CCNGG 1 cut(s) 903
TaaI ACNGT 5 cut(s) 137, 149, 329, 559, 1127
TaqII GACCGA 1 cut(s) 234
TatI WGTACW 4 cut(s) 118, 324, 649, 867
TauI GCSGC 3 cut(s) 111, 156, 474
TfiI GAWTC 1 cut(s) 402
Tru1I TTAA 3 cut(s) 381, 510, 1201
Tru9I TTAA 3 cut(s) 381, 510, 1201
TscAI CASTG 1 cut(s) 736
TseFI GTSAC 1 cut(s) 389
TseI GCWGC 6 cut(s) 92, 105, 182, 185, 704, 1172
Tsp45I GTSAC 1 cut(s) 389
TspDTI ATGAA 2 cut(s) 18, 27
TspRI CASTG 1 cut(s) 736
Van91I CCANNNNNTGG 1 cut(s) 288
VneI GTGCAC 1 cut(s) 330
VpaK11BI GGWCC 6 cut(s) 217, 296, 433, 545, 911, 1152
XapI RAATTY 4 cut(s) 958, 1015, 1024, 1075
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 2 cut(s) 211, 365
XspI CTAG 3 cut(s) 576, 656, 938
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.