Rmu_sc0005712.1_g000021

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005712.1
OG0013780
Physical Location & Seq
Forward (+)
115285 .. 116160
876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005712.1_g000021.1.cds

Sequence Viewer

Length: 876 bp
atgccgtactttaacccattttctgaacccggaagacgtcgactgcacgcaagggctgacgatgctgacctcggactaccgcgacacatatggcaatacattctaatgttcgtttccgcggatgacttgttcacaatacttgactgcaggattgtctgcaggacattctgcacgctcattgatgattacaatgtgtattcctgcatcaacatggctgatataggtggctggaacgacattcaattacctttggaagcggttcagggtcgccgcttatttgccctccaatgctttcgggcgaataatccggaaaccctgttccgccatgcagtccgaacaatgaggcgatcgggcgacattcgcttggccatgcagttcctagtaatggcatgcagggagggtgtaatcttcagaagtagcacttttaggtggatttcctaccatggaccagcgttttatgtcttgagcatgttgcagataatatgtggaaatccggacgtccaaagggccgccgtaggaaagttggtcagtcgattggactggaggcaaagcgggtggagtgcagagtcctgtcgagagttatgtgactgggaggtgtttgtgggaggaccggcgcaccctgatatcaggaaacacattccccctgacggtcccacccttccagacagatgtagagattgcagtcaagagaatttcggccgtccaatgggtgaaagaatgtggtatatcgatggcgacaccaacaaaggagagtgctgtgatagatgcaagtgcttggtcgacgcggtgttcttctcctgccaattatggaactgggcggcatcgagcgtggacagattgggacaacctcacagtgaggacgacgagtacccctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

291

Amino Acids

33.54

Weight (kDa)

5.99

Isoelectric Point (pI)

52.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 40, 501
AccI GTMKAC 2 cut(s) 40, 780
AccII CGCG 3 cut(s) 82, 119, 785
AccIII TCCGGA 2 cut(s) 307, 493
AcoI YGGCCR 2 cut(s) 366, 697
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 37, 498
AfaI GTAC 2 cut(s) 8, 869
AfiI CCNNNNNNNGG 3 cut(s) 385, 647, 706
AgsI TTSAA 1 cut(s) 242
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 302, 334
Aor13HI TCCGGA 2 cut(s) 307, 493
AoxI GGCC 3 cut(s) 366, 507, 697
ApoI RAATTY 1 cut(s) 691
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 4 cut(s) 446, 507, 608, 650
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 722
AvaII GGWCC 3 cut(s) 446, 608, 650
BalI TGGCCA 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 40
BccI CCATC 1 cut(s) 725
BceAI ACGGC 2 cut(s) 497, 684
BcnI CCSGG 1 cut(s) 30
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 2 cut(s) 145, 157
BisI GCNGC 3 cut(s) 271, 510, 819
BlsI GCNGC 3 cut(s) 272, 511, 820
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 3 cut(s) 446, 608, 650
BmgT120I GGNCC 4 cut(s) 446, 507, 608, 650
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 1 cut(s) 30
BmrI ACTGGG 2 cut(s) 598, 823
BmsI GCATC 4 cut(s) 52, 213, 755, 830
BmuI ACTGGG 2 cut(s) 598, 823
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 30
Bsa29I ATCGAT 1 cut(s) 729
BsaHI GRCGYC 2 cut(s) 37, 498
BsaJI CCNNGG 3 cut(s) 70, 117, 442
BsaWI WCCGGW 2 cut(s) 307, 493
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 647, 706
Bse118I RCCGGY 1 cut(s) 610
Bse1I ACTGG 3 cut(s) 545, 593, 818
BseAI TCCGGA 2 cut(s) 307, 493
BseCI ATCGAT 1 cut(s) 729
BseDI CCNNGG 3 cut(s) 70, 117, 442
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 3 cut(s) 385, 647, 706
BseNI ACTGG 3 cut(s) 545, 593, 818
BseX3I CGGCCG 1 cut(s) 697
BsgI GTGCAG 3 cut(s) 29, 154, 582
Bsh1236I CGCG 3 cut(s) 82, 119, 785
Bsh1285I CGRYCG 2 cut(s) 350, 700
BshFI GGCC 3 cut(s) 368, 509, 699
BshVI ATCGAT 1 cut(s) 729
BsiEI CGRYCG 2 cut(s) 350, 700
BsiSI CCGG 4 cut(s) 30, 308, 494, 611
BslFI GGGAC 2 cut(s) 636, 855
BslI CCNNNNNNNGG 3 cut(s) 385, 647, 706
BsmFI GGGAC 2 cut(s) 636, 855
BsnI GGCC 3 cut(s) 368, 509, 699
Bsp13I TCCGGA 2 cut(s) 307, 493
Bsp143I GATC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 442
BspANI GGCC 3 cut(s) 368, 509, 699
BspDI ATCGAT 1 cut(s) 729
BspEI TCCGGA 2 cut(s) 307, 493
BspFNI CGCG 3 cut(s) 82, 119, 785
BspLI GGNNCC 1 cut(s) 652
BspMAI CTGCAG 2 cut(s) 149, 161
BsrFI RCCGGY 1 cut(s) 610
BsrI ACTGG 3 cut(s) 545, 593, 818
BssAI RCCGGY 1 cut(s) 610
BssECI CCNNGG 3 cut(s) 70, 117, 442
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 2 cut(s) 37, 498
BssT1I CCWWGG 1 cut(s) 442
Bst4CI ACNGT 2 cut(s) 650, 854
BstACI GRCGYC 2 cut(s) 37, 498
BstC8I GCNNGC 3 cut(s) 48, 173, 391
BstDSI CCRYGG 2 cut(s) 117, 442
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 3 cut(s) 82, 119, 785
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 1 cut(s) 350
BstMBI GATC 1 cut(s) 347
BstMCI CGRYCG 2 cut(s) 350, 700
BstMWI GCNNNNNNNGC 2 cut(s) 62, 360
BstNSI RCATGY 2 cut(s) 393, 472
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 145, 157
BstUI CGCG 3 cut(s) 82, 119, 785
BstV2I GAAGAC 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 697
Bsu15I ATCGAT 1 cut(s) 729
BsuRI GGCC 3 cut(s) 368, 509, 699
BsuTUI ATCGAT 1 cut(s) 729
BtgI CCRYGG 2 cut(s) 117, 442
BtsCI GGATG 1 cut(s) 127
BtsIMutI CAGTG 1 cut(s) 859
Cac8I GCNNGC 3 cut(s) 48, 173, 391
CfoI GCGC 1 cut(s) 616
Cfr10I RCCGGY 1 cut(s) 610
Cfr13I GGNCC 4 cut(s) 446, 507, 608, 650
Cfr42I CCGCGG 1 cut(s) 120
ClaI ATCGAT 1 cut(s) 729
CseI GACGC 1 cut(s) 791
Csp6I GTAC 2 cut(s) 7, 868
CspCI CAANNNNNGTGG 2 cut(s) 536, 571
CviAII CATG 6 cut(s) 211, 326, 370, 390, 443, 469
CviJI RGCY 6 cut(s) 56, 215, 228, 368, 509, 699
CviKI_1 RGCY 6 cut(s) 56, 215, 228, 368, 509, 699
CviQI GTAC 2 cut(s) 7, 868
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
EaeI YGGCCR 2 cut(s) 366, 697
EagI CGGCCG 1 cut(s) 697
EciI GGCGGA 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 442
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 3 cut(s) 446, 608, 650
Eco52I CGGCCG 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 394
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 442
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 6 cut(s) 214, 329, 373, 393, 446, 472
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FaqI GGGAC 2 cut(s) 636, 855
FatI CATG 6 cut(s) 210, 325, 369, 389, 442, 468
FauI CCCGC 1 cut(s) 545
FauNDI CATATG 1 cut(s) 89
FblI GTMKAC 2 cut(s) 40, 780
Fnu4HI GCNGC 3 cut(s) 271, 510, 819
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 3 cut(s) 271, 510, 819
FspBI CTAG 1 cut(s) 380
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 271, 510, 819
GsuI CTGGAG 1 cut(s) 562
HaeIII GGCC 3 cut(s) 368, 509, 699
HapII CCGG 4 cut(s) 30, 308, 494, 611
HgaI GACGC 1 cut(s) 791
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 2 cut(s) 37, 498
Hin1II CATG 6 cut(s) 214, 329, 373, 393, 446, 472
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 2 cut(s) 41, 781
HindII GTYRAC 2 cut(s) 41, 781
HinfI GANTC 1 cut(s) 566
HpaII CCGG 4 cut(s) 30, 308, 494, 611
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 4 cut(s) 41, 132, 781, 832
Hpy188I TCNGA 4 cut(s) 25, 74, 335, 413
Hpy188III TCNNGA 7 cut(s) 308, 463, 494, 575, 628, 662, 686
Hpy8I GTNNAC 4 cut(s) 41, 132, 781, 832
Hpy99I CGWCG 3 cut(s) 42, 785, 866
HpyAV CCTTC 1 cut(s) 668
HpyCH4III ACNGT 2 cut(s) 650, 854
HpyCH4IV ACGT 2 cut(s) 37, 498
HpyF10VI GCNNNNNNNGC 2 cut(s) 62, 360
HpySE526I ACGT 2 cut(s) 37, 498
Hsp92I GRCGYC 2 cut(s) 37, 498
Hsp92II CATG 6 cut(s) 214, 329, 373, 393, 446, 472
HspAI GCGC 1 cut(s) 614
Kpn2I TCCGGA 2 cut(s) 307, 493
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 1 cut(s) 347
LweI GCATC 4 cut(s) 52, 213, 755, 830
MaeI CTAG 1 cut(s) 380
MaeII ACGT 2 cut(s) 37, 498
MaeIII GTNAC 1 cut(s) 584
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 3 cut(s) 45, 400, 784
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 3 cut(s) 242, 691, 803
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 575
MnlI CCTC 9 cut(s) 80, 293, 336, 391, 537, 586, 599, 850, 858
Mox20I TGGCCA 1 cut(s) 368
MroI TCCGGA 2 cut(s) 307, 493
MroXI GAANNNNTTC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 2 cut(s) 104, 209
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 4 cut(s) 30, 308, 494, 611
MspR9I CCNGG 1 cut(s) 30
MvnI CGCG 3 cut(s) 82, 119, 785
MwoI GCNNNNNNNGC 2 cut(s) 62, 360
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 89
NdeII GATC 1 cut(s) 347
NlaIII CATG 6 cut(s) 214, 329, 373, 393, 446, 472
NlaIV GGNNCC 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 584
NspI RCATGY 2 cut(s) 393, 472
PaeI GCATGC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 258
PkrI GCNGC 3 cut(s) 272, 511, 820
Ple19I CGATCG 1 cut(s) 350
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 4 cut(s) 446, 507, 608, 650
PstI CTGCAG 2 cut(s) 149, 161
PvuI CGATCG 1 cut(s) 350
RsaI GTAC 2 cut(s) 8, 869
RsaNI GTAC 2 cut(s) 7, 868
RseI CAYNNNNRTG 2 cut(s) 104, 209
SacII CCGCGG 1 cut(s) 120
SalI GTCGAC 2 cut(s) 39, 779
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 3 cut(s) 271, 510, 819
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 4 cut(s) 446, 507, 608, 650
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 30
SetI ASST 8 cut(s) 40, 72, 226, 250, 431, 501, 597, 850
SfaNI GCATC 4 cut(s) 52, 213, 755, 830
SfcI CTRYAG 2 cut(s) 145, 157
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 3 cut(s) 446, 608, 650
SmiMI CAYNNNNRTG 2 cut(s) 104, 209
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SphI GCATGC 1 cut(s) 393
Sse9I AATT 3 cut(s) 242, 691, 803
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 2 cut(s) 650, 854
TaiI ACGT 2 cut(s) 40, 501
TaqI TCGA 6 cut(s) 40, 532, 574, 729, 780, 824
TasI AATT 3 cut(s) 242, 691, 803
TauI GCSGC 3 cut(s) 273, 512, 821
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 1 cut(s) 859
TseFI GTSAC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 584
TspRI CASTG 1 cut(s) 859
VpaK11BI GGWCC 3 cut(s) 446, 608, 650
XapI RAATTY 1 cut(s) 691
XceI RCATGY 2 cut(s) 393, 472
XmiI GTMKAC 2 cut(s) 40, 780
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 1 cut(s) 380
ZraI GACGTC 2 cut(s) 38, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.