Rh4BG254700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0013780
Physical Location & Seq
Forward (+)
42550038 .. 42550877
840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG254700.1

Sequence Viewer

Length: 840 bp
ATGCCGTACTTTAACCCATTTTCTGAACCCGGAAGACGTCGACTGCACGTAAGGGCTGACGATGCTGACCTCGGACTACCGCTACACATATGGCAATACATTCTAATGTTCGTTGCCGCGGATGACTTGTTCAGAATACTGGACTGCAGGACTGTCTGCAGGACATTCCGCACGCTCATTGATGATTACAATGTGTATTCATGCATCAACATGGCTGATATAGGTGGCTGGAACGACATTCAATTTCCTTTGGAAGCAGTTCAGGGTCGCCTCTTATTTGCCATTCAATGCTTTCGGGCGAATAATCCGGAAGCCCTGTTCCGCCATGCAGTCCAAACAATGAGGCGATCGGGCGACATTCCATTGGCCATGCAGTTCCTAGAAATGGCATGCAGGGAGGGTGTAATCTTCAGAAGTAGCACTTTTAGGTGGATTCCCTACCATGGACCAGCGTTTTATGTCTTGAGCATGTTGCAGATAATATGTGGAAATCCGGATGTCCAAAGGGCCGCCGTAGAAAAGATGGTCAGTCGCCTAGACTGGAGGCAAAGCGGGTGGAGTGCAGAGTCCTGTCGAGAGTTATGTGACTGGGAGGTGTTTGTGGGAGGACCTGCGCACCCTGATATCAGGAATCACATACCCCCTGACGGTCCCACCCTTCCAGAAAGATGTAGCGATTGCGGTCAAGAGAATTTCGGCCGTCCAATGGGTGAAAGAATGTGGTACATCGACGGCGACACCAACAAAGGAGACTGTTGTGATAGATGCGGTGTTCTTCTCCTGGAAATTATGGACCTGGGCGGCATCGAGCGTGGAACGAATGGGACAACCACACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

279

Amino Acids

31.87

Weight (kDa)

5.28

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 91 - 196 4.7e-07 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 40
Acc16I TGCGCA 1 cut(s) 615
Acc36I ACCTGC 1 cut(s) 619
AccI GTMKAC 1 cut(s) 40
AccII CGCG 1 cut(s) 119
AccIII TCCGGA 2 cut(s) 307, 493
AcoI YGGCCR 2 cut(s) 366, 697
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 37
AdeI CACNNNGTG 1 cut(s) 837
AfaI GTAC 2 cut(s) 8, 725
AfiI CCNNNNNNNGG 4 cut(s) 385, 443, 647, 706
AgsI TTSAA 2 cut(s) 242, 287
AjnI CCWGG 2 cut(s) 780, 795
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 302, 334
Alw26I GTCTC 1 cut(s) 744
Aor13HI TCCGGA 2 cut(s) 307, 493
AoxI GGCC 3 cut(s) 366, 507, 697
ApoI RAATTY 1 cut(s) 691
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 616
AspS9I GGNCC 5 cut(s) 446, 507, 608, 650, 793
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 722
AvaII GGWCC 4 cut(s) 446, 608, 650, 793
BalI TGGCCA 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 40
BccI CCATC 1 cut(s) 517
BceAI ACGGC 3 cut(s) 497, 684, 748
BciT130I CCWGG 2 cut(s) 782, 797
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 744
BfaI CTAG 2 cut(s) 380, 536
BfmI CTRYAG 2 cut(s) 145, 157
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 3 cut(s) 117, 510, 802
BlsI GCNGC 3 cut(s) 118, 511, 803
Bme1390I CCNGG 3 cut(s) 30, 782, 797
Bme18I GGWCC 4 cut(s) 446, 608, 650, 793
BmgT120I GGNCC 5 cut(s) 446, 507, 608, 650, 793
BmiI GGNNCC 1 cut(s) 652
BmrFI CCNGG 3 cut(s) 30, 782, 797
BmrI ACTGGG 1 cut(s) 598
BmsI GCATC 4 cut(s) 52, 213, 755, 813
BmuI ACTGGG 1 cut(s) 598
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 562
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 49
BsaHI GRCGYC 1 cut(s) 37
BsaJI CCNNGG 4 cut(s) 70, 117, 442, 796
BsaWI WCCGGW 2 cut(s) 307, 493
Bsc4I CCNNNNNNNGG 4 cut(s) 385, 443, 647, 706
Bse1I ACTGG 3 cut(s) 144, 545, 593
BseAI TCCGGA 2 cut(s) 307, 493
BseBI CCWGG 2 cut(s) 782, 797
BseDI CCNNGG 4 cut(s) 70, 117, 442, 796
BseGI GGATG 2 cut(s) 127, 502
BseLI CCNNNNNNNGG 4 cut(s) 385, 443, 647, 706
BseNI ACTGG 3 cut(s) 144, 545, 593
BseX3I CGGCCG 1 cut(s) 697
BsgI GTGCAG 2 cut(s) 29, 582
Bsh1236I CGCG 1 cut(s) 119
Bsh1285I CGRYCG 2 cut(s) 350, 700
BshFI GGCC 3 cut(s) 368, 509, 699
BsiEI CGRYCG 2 cut(s) 350, 700
BsiSI CCGG 3 cut(s) 30, 308, 494
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 4 cut(s) 385, 443, 647, 706
BsmAI GTCTC 1 cut(s) 744
BsmFI GGGAC 1 cut(s) 636
BsnI GGCC 3 cut(s) 368, 509, 699
Bsp13I TCCGGA 2 cut(s) 307, 493
Bsp143I GATC 1 cut(s) 347
Bsp19I CCATGG 1 cut(s) 442
BspANI GGCC 3 cut(s) 368, 509, 699
BspEI TCCGGA 2 cut(s) 307, 493
BspFNI CGCG 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 652
BspMAI CTGCAG 2 cut(s) 149, 161
BspMI ACCTGC 1 cut(s) 619
BsrI ACTGG 3 cut(s) 144, 545, 593
BssECI CCNNGG 4 cut(s) 70, 117, 442, 796
BssMI GATC 1 cut(s) 347
BssNI GRCGYC 1 cut(s) 37
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 782, 797
Bst4CI ACNGT 4 cut(s) 154, 650, 755, 837
BstACI GRCGYC 1 cut(s) 37
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 173, 391
BstDSI CCRYGG 2 cut(s) 117, 442
BstF5I GGATG 2 cut(s) 127, 502
BstFNI CGCG 1 cut(s) 119
BstHHI GCGC 1 cut(s) 616
BstKTI GATC 1 cut(s) 350
BstMAI GTCTC 1 cut(s) 744
BstMBI GATC 1 cut(s) 347
BstMCI CGRYCG 2 cut(s) 350, 700
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 2 cut(s) 782, 797
BstNSI RCATGY 2 cut(s) 393, 472
BstSCI CCNGG 3 cut(s) 28, 780, 795
BstSFI CTRYAG 2 cut(s) 145, 157
BstUI CGCG 1 cut(s) 119
BstV2I GAAGAC 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 697
BsuRI GGCC 3 cut(s) 368, 509, 699
BtgI CCRYGG 2 cut(s) 117, 442
BtsCI GGATG 2 cut(s) 127, 502
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 2 cut(s) 173, 391
CfoI GCGC 1 cut(s) 616
Cfr13I GGNCC 5 cut(s) 446, 507, 608, 650, 793
Cfr42I CCGCGG 1 cut(s) 120
Csp6I GTAC 2 cut(s) 7, 724
CspCI CAANNNNNGTGG 2 cut(s) 536, 571
CviAII CATG 7 cut(s) 201, 211, 326, 370, 390, 443, 469
CviJI RGCY 7 cut(s) 56, 215, 228, 314, 368, 509, 699
CviKI_1 RGCY 7 cut(s) 56, 215, 228, 314, 368, 509, 699
CviQI GTAC 2 cut(s) 7, 724
DpnI GATC 1 cut(s) 349
DpnII GATC 1 cut(s) 347
DraIII CACNNNGTG 1 cut(s) 837
EaeI YGGCCR 2 cut(s) 366, 697
EagI CGGCCG 1 cut(s) 697
EciI GGCGGA 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 697
Eco130I CCWWGG 1 cut(s) 442
Eco32I GATATC 1 cut(s) 625
Eco47I GGWCC 4 cut(s) 446, 608, 650, 793
Eco52I CGGCCG 1 cut(s) 697
Eco57I CTGAAG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 2 cut(s) 780, 795
EcoRV GATATC 1 cut(s) 625
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 7 cut(s) 204, 214, 329, 373, 393, 446, 472
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FaqI GGGAC 1 cut(s) 636
FatI CATG 7 cut(s) 200, 210, 325, 369, 389, 442, 468
FauI CCCGC 1 cut(s) 545
FauNDI CATATG 1 cut(s) 89
FblI GTMKAC 1 cut(s) 40
Fnu4HI GCNGC 3 cut(s) 117, 510, 802
FokI GGATG 2 cut(s) 134, 509
Fsp4HI GCNGC 3 cut(s) 117, 510, 802
FspBI CTAG 2 cut(s) 380, 536
FspI TGCGCA 1 cut(s) 615
GlaI GCGC 1 cut(s) 615
GluI GCNGC 3 cut(s) 117, 510, 802
GsuI CTGGAG 1 cut(s) 562
HaeIII GGCC 3 cut(s) 368, 509, 699
HapII CCGG 3 cut(s) 30, 308, 494
HhaI GCGC 1 cut(s) 616
Hin1I GRCGYC 1 cut(s) 37
Hin1II CATG 7 cut(s) 204, 214, 329, 373, 393, 446, 472
Hin6I GCGC 1 cut(s) 614
HinP1I GCGC 1 cut(s) 614
HincII GTYRAC 1 cut(s) 41
HindII GTYRAC 1 cut(s) 41
HinfI GANTC 3 cut(s) 433, 566, 631
HpaII CCGG 3 cut(s) 30, 308, 494
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 1 cut(s) 41
Hpy188I TCNGA 4 cut(s) 25, 74, 134, 413
Hpy188III TCNNGA 7 cut(s) 308, 463, 494, 575, 628, 662, 686
Hpy8I GTNNAC 1 cut(s) 41
Hpy99I CGWCG 2 cut(s) 42, 734
HpyAV CCTTC 1 cut(s) 668
HpyCH4III ACNGT 4 cut(s) 154, 650, 755, 837
HpyCH4IV ACGT 2 cut(s) 37, 48
HpyCH4V TGCA 9 cut(s) 46, 147, 159, 204, 329, 373, 393, 475, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 2 cut(s) 37, 48
Hsp92I GRCGYC 1 cut(s) 37
Hsp92II CATG 7 cut(s) 204, 214, 329, 373, 393, 446, 472
HspAI GCGC 1 cut(s) 614
Kpn2I TCCGGA 2 cut(s) 307, 493
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 1 cut(s) 347
LweI GCATC 4 cut(s) 52, 213, 755, 813
MaeI CTAG 2 cut(s) 380, 536
MaeII ACGT 2 cut(s) 37, 48
MaeIII GTNAC 1 cut(s) 584
MalI GATC 1 cut(s) 349
MboI GATC 1 cut(s) 347
MboII GAAGA 3 cut(s) 45, 400, 767
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 3 cut(s) 242, 691, 786
MluNI TGGCCA 1 cut(s) 368
MlyI GAGTC 1 cut(s) 575
MnlI CCTC 7 cut(s) 80, 281, 336, 391, 537, 586, 599
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 206
MroI TCCGGA 2 cut(s) 307, 493
MroXI GAANNNNTTC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 2 cut(s) 104, 209
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 3 cut(s) 30, 308, 494
MspR9I CCNGG 3 cut(s) 30, 782, 797
MvaI CCWGG 2 cut(s) 782, 797
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 89
NdeII GATC 1 cut(s) 347
NlaIII CATG 7 cut(s) 204, 214, 329, 373, 393, 446, 472
NlaIV GGNNCC 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 584
NsbI TGCGCA 1 cut(s) 615
NsiI ATGCAT 1 cut(s) 206
NspI RCATGY 2 cut(s) 393, 472
PaeI GCATGC 1 cut(s) 393
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 433, 631
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 3 cut(s) 118, 511, 803
Ple19I CGATCG 1 cut(s) 350
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
Ppu21I YACGTR 1 cut(s) 49
PpuMI RGGWCCY 1 cut(s) 608
Psp5II RGGWCCY 1 cut(s) 608
Psp6I CCWGG 2 cut(s) 780, 795
PspGI CCWGG 2 cut(s) 780, 795
PspN4I GGNNCC 1 cut(s) 652
PspPI GGNCC 5 cut(s) 446, 507, 608, 650, 793
PspPPI RGGWCCY 1 cut(s) 608
PstI CTGCAG 2 cut(s) 149, 161
PvuI CGATCG 1 cut(s) 350
RsaI GTAC 2 cut(s) 8, 725
RsaNI GTAC 2 cut(s) 7, 724
RseI CAYNNNNRTG 2 cut(s) 104, 209
SacII CCGCGG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 3 cut(s) 117, 510, 802
Sau3AI GATC 1 cut(s) 347
Sau96I GGNCC 5 cut(s) 446, 507, 608, 650, 793
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 3 cut(s) 30, 782, 797
SetI ASST 8 cut(s) 40, 51, 72, 226, 431, 597, 613, 798
SfaNI GCATC 4 cut(s) 52, 213, 755, 813
SfcI CTRYAG 2 cut(s) 145, 157
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 4 cut(s) 446, 608, 650, 793
SmiMI CAYNNNNRTG 2 cut(s) 104, 209
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SphI GCATGC 1 cut(s) 393
Sse9I AATT 3 cut(s) 242, 691, 786
SspMI CTAG 2 cut(s) 380, 536
StyD4I CCNGG 3 cut(s) 28, 780, 795
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 4 cut(s) 154, 650, 755, 837
TaiI ACGT 2 cut(s) 40, 51
TaqI TCGA 4 cut(s) 40, 574, 729, 807
TasI AATT 3 cut(s) 242, 691, 786
TauI GCSGC 3 cut(s) 119, 512, 804
TfiI GAWTC 2 cut(s) 433, 631
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseFI GTSAC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 584
TspDTI ATGAA 1 cut(s) 189
VpaK11BI GGWCC 4 cut(s) 446, 608, 650, 793
XapI RAATTY 1 cut(s) 691
XceI RCATGY 2 cut(s) 393, 472
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 2 cut(s) 380, 536
ZraI GACGTC 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.