Rh2AG642200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013780
Physical Location & Seq
Reverse (-)
86491831 .. 86492394
564 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG642200.1

Sequence Viewer

Length: 564 bp
ATGTTGTACTTTAACCCATTTTCTGAACCCGGAAGACGTCGACTGCACGCAAGGGCTGACGATGCTGACCTCGGACTACCGCTACACATATGGCAATACATTCTAATGTTCGTTGCCGCGGATGACTTGTTCAGAATACTGGACTGCAGGACTGTCTACAGGACATTCTGCAAGCTCATTGATGATTACAATGTGTATTCATGCATCAACATGGCTGATATAGGTGGCTGGAACGACATTCAATTTCCTTTGGAAGCAGTTCAGGGTCGCAGCTTATTTGCCCTCCAATGCTTTCGAGCGAATAATCCGGAAGCCCTGTTCCGCCATGCAGTCCGAACAATGAGGCAATCGGGCAACATTCCATTGGCCATACAGTTCCTAGAAATGGCATGCAGGGAGGGTGTAATCTTCAGAAGTAGCACTTTTAGGTGGATTCCCTACCATGGACCGGCGTTTTATGTCTTGAGCATGTTGCAGATAATATGTGGAAATCCGGACGTCCAAAGGGCCACCATAGAAAAGATGGTCAGTCGCTGGACTGGAGGCAAAGCAGGTGGAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.48

Weight (kDa)

8.58

Isoelectric Point (pI)

56.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 88 - 134 1.5e-06 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 542
AatII GACGTC 2 cut(s) 40, 501
Acc36I ACCTGC 1 cut(s) 542
AccI GTMKAC 2 cut(s) 40, 156
AccII CGCG 1 cut(s) 119
AccIII TCCGGA 2 cut(s) 307, 493
AciI CCGC 4 cut(s) 80, 117, 119, 322
AcoI YGGCCR 1 cut(s) 366
AcuI CTGAAG 1 cut(s) 394
AcyI GRCGYC 2 cut(s) 37, 498
AfaI GTAC 1 cut(s) 8
AfiI CCNNNNNNNGG 3 cut(s) 385, 443, 448
AgsI TTSAA 1 cut(s) 242
AloI GAACNNNNNNTCC 4 cut(s) 113, 145, 302, 334
AluBI AGCT 2 cut(s) 175, 273
AluI AGCT 2 cut(s) 175, 273
AlwNI CAGNNNCTG 1 cut(s) 534
Aor13HI TCCGGA 2 cut(s) 307, 493
AoxI GGCC 2 cut(s) 366, 507
ApeKI GCWGC 1 cut(s) 270
Asp700I GAANNNNTTC 1 cut(s) 258
AspS9I GGNCC 2 cut(s) 446, 507
AsuC2I CCSGG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 40
BbvI GCAGC 1 cut(s) 282
BccI CCATC 1 cut(s) 517
BcnI CCSGG 1 cut(s) 30
BfaI CTAG 1 cut(s) 380
BfmI CTRYAG 2 cut(s) 145, 157
BfuAI ACCTGC 1 cut(s) 542
BisI GCNGC 2 cut(s) 117, 271
BlsI GCNGC 2 cut(s) 118, 272
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 446, 507
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 2 cut(s) 52, 213
BpiI GAAGAC 1 cut(s) 40
BpmI CTGGAG 1 cut(s) 561
BpuEI CTTGAG 1 cut(s) 484
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 2 cut(s) 37, 498
BsaJI CCNNGG 3 cut(s) 70, 117, 442
BsaWI WCCGGW 2 cut(s) 307, 493
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 443, 448
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 2 cut(s) 144, 544
BseAI TCCGGA 2 cut(s) 307, 493
BseDI CCNNGG 3 cut(s) 70, 117, 442
BseGI GGATG 1 cut(s) 127
BseLI CCNNNNNNNGG 3 cut(s) 385, 443, 448
BseNI ACTGG 2 cut(s) 144, 544
BseXI GCAGC 1 cut(s) 282
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 368, 509
BsiSI CCGG 4 cut(s) 30, 308, 449, 494
BslI CCNNNNNNNGG 3 cut(s) 385, 443, 448
BsnI GGCC 2 cut(s) 368, 509
Bsp13I TCCGGA 2 cut(s) 307, 493
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 4 cut(s) 80, 117, 119, 322
BspANI GGCC 2 cut(s) 368, 509
BspEI TCCGGA 2 cut(s) 307, 493
BspFNI CGCG 1 cut(s) 119
BspMAI CTGCAG 1 cut(s) 149
BspMI ACCTGC 1 cut(s) 542
BsrFI RCCGGY 1 cut(s) 448
BsrI ACTGG 2 cut(s) 144, 544
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 70, 117, 442
BssNI GRCGYC 2 cut(s) 37, 498
BssT1I CCWWGG 1 cut(s) 442
Bst4CI ACNGT 2 cut(s) 154, 375
BstACI GRCGYC 2 cut(s) 37, 498
BstC8I GCNNGC 3 cut(s) 48, 173, 391
BstDSI CCRYGG 2 cut(s) 117, 442
BstF5I GGATG 1 cut(s) 127
BstFNI CGCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNSI RCATGY 2 cut(s) 393, 472
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 145, 157
BstUI CGCG 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 40
BsuRI GGCC 2 cut(s) 368, 509
BtgI CCRYGG 2 cut(s) 117, 442
BtsCI GGATG 1 cut(s) 127
BveI ACCTGC 1 cut(s) 542
Cac8I GCNNGC 3 cut(s) 48, 173, 391
CaiI CAGNNNCTG 1 cut(s) 534
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 446, 507
Cfr42I CCGCGG 1 cut(s) 120
Csp6I GTAC 1 cut(s) 7
CspCI CAANNNNNGTGG 1 cut(s) 535
CviAII CATG 6 cut(s) 201, 211, 326, 390, 443, 469
CviJI RGCY 8 cut(s) 56, 175, 215, 228, 273, 314, 368, 509
CviKI_1 RGCY 8 cut(s) 56, 175, 215, 228, 273, 314, 368, 509
CviQI GTAC 1 cut(s) 7
EaeI YGGCCR 1 cut(s) 366
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 1 cut(s) 442
Eco47I GGWCC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 394
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 6 cut(s) 204, 214, 329, 393, 446, 472
FalI AAGNNNNNCTT 2 cut(s) 406, 438
FatI CATG 6 cut(s) 200, 210, 325, 389, 442, 468
FauNDI CATATG 1 cut(s) 89
FblI GTMKAC 2 cut(s) 40, 156
Fnu4HI GCNGC 2 cut(s) 117, 271
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 2 cut(s) 117, 271
FspBI CTAG 1 cut(s) 380
GluI GCNGC 2 cut(s) 117, 271
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 2 cut(s) 368, 509
HapII CCGG 4 cut(s) 30, 308, 449, 494
Hin1I GRCGYC 2 cut(s) 37, 498
Hin1II CATG 6 cut(s) 204, 214, 329, 393, 446, 472
HincII GTYRAC 1 cut(s) 41
HindII GTYRAC 1 cut(s) 41
HinfI GANTC 1 cut(s) 433
HpaII CCGG 4 cut(s) 30, 308, 449, 494
Hpy166II GTNNAC 2 cut(s) 41, 157
Hpy188I TCNGA 5 cut(s) 25, 74, 134, 335, 413
Hpy188III TCNNGA 3 cut(s) 308, 463, 494
Hpy8I GTNNAC 2 cut(s) 41, 157
Hpy99I CGWCG 1 cut(s) 42
HpyCH4III ACNGT 2 cut(s) 154, 375
HpyCH4IV ACGT 2 cut(s) 37, 498
HpyCH4V TGCA 7 cut(s) 46, 147, 171, 204, 329, 393, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 2 cut(s) 37, 498
Hsp92I GRCGYC 2 cut(s) 37, 498
Hsp92II CATG 6 cut(s) 204, 214, 329, 393, 446, 472
Kpn2I TCCGGA 2 cut(s) 307, 493
KspI CCGCGG 1 cut(s) 120
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 2 cut(s) 52, 213
MaeI CTAG 1 cut(s) 380
MaeII ACGT 2 cut(s) 37, 498
MboII GAAGA 2 cut(s) 45, 400
MlsI TGGCCA 1 cut(s) 368
MluCI AATT 1 cut(s) 242
MluNI TGGCCA 1 cut(s) 368
MnlI CCTC 5 cut(s) 80, 293, 336, 391, 536
Mox20I TGGCCA 1 cut(s) 368
Mph1103I ATGCAT 1 cut(s) 206
MroI TCCGGA 2 cut(s) 307, 493
MroXI GAANNNNTTC 1 cut(s) 258
MscI TGGCCA 1 cut(s) 368
MseI TTAA 1 cut(s) 12
MslI CAYNNNNRTG 2 cut(s) 104, 209
Msp20I TGGCCA 1 cut(s) 368
MspA1I CMGCKG 1 cut(s) 119
MspI CCGG 4 cut(s) 30, 308, 449, 494
MspR9I CCNGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 119
MwoI GCNNNNNNNGC 1 cut(s) 62
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 89
NlaIII CATG 6 cut(s) 204, 214, 329, 393, 446, 472
NsiI ATGCAT 1 cut(s) 206
NspI RCATGY 2 cut(s) 393, 472
PaeI GCATGC 1 cut(s) 393
PaqCI CACCTGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 433
PkrI GCNGC 2 cut(s) 118, 272
PspPI GGNCC 2 cut(s) 446, 507
PstI CTGCAG 1 cut(s) 149
PstNI CAGNNNCTG 1 cut(s) 534
RsaI GTAC 1 cut(s) 8
RsaNI GTAC 1 cut(s) 7
RseI CAYNNNNRTG 2 cut(s) 104, 209
SacII CCGCGG 1 cut(s) 120
SalI GTCGAC 1 cut(s) 39
SaqAI TTAA 1 cut(s) 12
SatI GCNGC 2 cut(s) 117, 271
Sau96I GGNCC 2 cut(s) 446, 507
ScrFI CCNGG 1 cut(s) 30
SetI ASST 8 cut(s) 40, 72, 177, 226, 275, 431, 501, 556
SfaNI GCATC 2 cut(s) 52, 213
SfcI CTRYAG 2 cut(s) 145, 157
Sfr303I CCGCGG 1 cut(s) 120
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 2 cut(s) 104, 209
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SphI GCATGC 1 cut(s) 393
Sse9I AATT 1 cut(s) 242
SsiI CCGC 4 cut(s) 80, 117, 119, 322
SspMI CTAG 1 cut(s) 380
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 2 cut(s) 154, 375
TaiI ACGT 2 cut(s) 40, 501
TaqI TCGA 2 cut(s) 40, 295
TasI AATT 1 cut(s) 242
TatI WGTACW 1 cut(s) 6
TauI GCSGC 1 cut(s) 119
TfiI GAWTC 1 cut(s) 433
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TseI GCWGC 1 cut(s) 270
TspDTI ATGAA 1 cut(s) 189
VpaK11BI GGWCC 1 cut(s) 446
XceI RCATGY 2 cut(s) 393, 472
XcmI CCANNNNNNNNNTGG 1 cut(s) 520
XmiI GTMKAC 2 cut(s) 40, 156
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 1 cut(s) 380
ZraI GACGTC 2 cut(s) 38, 499
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.