Rh5DG239400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013780
Physical Location & Seq
Reverse (-)
27363350 .. 27363688
339 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG239400.1

Sequence Viewer

Length: 339 bp
ATGCCATACTTCAAGCCACTGTCTCTGCCTGGAAGGCGCCGAAGACTCAACCCGAGAGCGGCCAATGACTTCACACTGCCTCTCGTCATATGGCAATATATTCTTCAGTTTGTTGCCGCCGATGACCTATACACAATTCTAAAGTGTAGGAGTGTTTGTAAGACTTTTATGAGACTCATTGATGACTTCCATGTCTATTCATGCATAGACATGGCGGGCATATGTGGTCCTTGGGATATTGACATTTGTGAATCATCGAAAGTGACGCGACGTCGCCTGTTTGCTAAGCAATGCTTGGAGGCGAATAATCCGGAAGCACTGTTCCGTCAGGCATTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

13.1

Weight (kDa)

9.03

Isoelectric Point (pI)

56.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 26 - 66 3e-06 F-box domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 274
AccB1I GGYRCC 1 cut(s) 36
AccBSI CCGCTC 1 cut(s) 59
AccII CGCG 1 cut(s) 268
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 3 cut(s) 59, 117, 215
AcoI YGGCCR 1 cut(s) 60
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 37, 271
AfiI CCNNNNNNNGG 1 cut(s) 58
AgsI TTSAA 1 cut(s) 13
AhdI GACNNNNNGTC 1 cut(s) 270
AjnI CCWGG 1 cut(s) 28
AloI GAACNNNNNNTCC 2 cut(s) 305, 337
Alw26I GTCTC 2 cut(s) 27, 166
Ama87I CYCGRG 1 cut(s) 52
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 1 cut(s) 60
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 227
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 1 cut(s) 227
BanI GGYRCC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 30
BcoDI GTCTC 2 cut(s) 27, 166
BfoI RGCGCY 1 cut(s) 40
BglI GCCNNNNNGGC 1 cut(s) 34
BisI GCNGC 2 cut(s) 60, 117
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 2 cut(s) 61, 118
Bme1390I CCNGG 1 cut(s) 30
Bme18I GGWCC 1 cut(s) 227
BmeRI GACNNNNNGTC 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 1 cut(s) 227
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 1 cut(s) 30
BpiI GAAGAC 1 cut(s) 49
Bpu1102I GCTNAGC 1 cut(s) 285
BsaHI GRCGYC 2 cut(s) 37, 271
BsaJI CCNNGG 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 310
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 296
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 1 cut(s) 230
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 1 cut(s) 62
BshNI GGYRCC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 1 cut(s) 311
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 2 cut(s) 27, 166
BsmI GAATGC 1 cut(s) 332
BsnI GGCC 1 cut(s) 62
BsoBI CYCGRG 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 310
Bsp1720I GCTNAGC 1 cut(s) 285
BspACI CCGC 3 cut(s) 59, 117, 215
BspANI GGCC 1 cut(s) 62
BspEI TCCGGA 1 cut(s) 310
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 38
BspT107I GGYRCC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 1 cut(s) 296
BssECI CCNNGG 1 cut(s) 230
BssNI GRCGYC 2 cut(s) 37, 271
BssT1I CCWWGG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 21, 321
BstACI GRCGYC 2 cut(s) 37, 271
BstC8I GCNNGC 1 cut(s) 217
BstDEI CTNAG 1 cut(s) 285
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstMAI GTCTC 2 cut(s) 27, 166
BstMWI GCNNNNNNNGC 1 cut(s) 34
BstNI CCWGG 1 cut(s) 30
BstSCI CCNGG 1 cut(s) 28
BstUI CGCG 1 cut(s) 268
BstV2I GAAGAC 1 cut(s) 49
BsuRI GGCC 1 cut(s) 62
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 17, 74, 317
Cac8I GCNNGC 1 cut(s) 217
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 274
CviAII CATG 3 cut(s) 191, 201, 211
CviJI RGCY 2 cut(s) 16, 62
CviKI_1 RGCY 2 cut(s) 16, 62
DdeI CTNAG 1 cut(s) 285
DinI GGCGCC 1 cut(s) 38
DrdI GACNNNNNNGTC 1 cut(s) 191
DriI GACNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 60
Eam1105I GACNNNNNGTC 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 230
Eco47I GGWCC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 230
EcoT22I ATGCAT 1 cut(s) 206
EgeI GGCGCC 1 cut(s) 38
EheI GGCGCC 1 cut(s) 38
ErhI CCWWGG 1 cut(s) 230
FaeI CATG 3 cut(s) 194, 204, 214
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FatI CATG 3 cut(s) 190, 200, 210
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 2 cut(s) 89, 221
Fnu4HI GCNGC 2 cut(s) 60, 117
Fsp4HI GCNGC 2 cut(s) 60, 117
GlaI GCGC 1 cut(s) 38
GluI GCNGC 2 cut(s) 60, 117
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 1 cut(s) 62
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 274
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 37, 271
Hin1II CATG 3 cut(s) 194, 204, 214
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HinfI GANTC 3 cut(s) 45, 174, 251
HpaII CCGG 1 cut(s) 311
Hpy188I TCNGA 1 cut(s) 338
Hpy188III TCNNGA 1 cut(s) 311
Hpy99I CGWCG 2 cut(s) 273, 276
HpyAV CCTTC 1 cut(s) 27
HpyCH4III ACNGT 2 cut(s) 21, 321
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 1 cut(s) 204
HpyF10VI GCNNNNNNNGC 1 cut(s) 34
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 271
Hsp92I GRCGYC 2 cut(s) 37, 271
Hsp92II CATG 3 cut(s) 194, 204, 214
HspAI GCGC 1 cut(s) 37
KasI GGCGCC 1 cut(s) 36
Kpn2I TCCGGA 1 cut(s) 310
LpnPI CCDG 5 cut(s) 15, 42, 290, 314, 324
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 1 cut(s) 262
MbiI CCGCTC 1 cut(s) 59
MboII GAAGA 2 cut(s) 54, 95
MluCI AATT 1 cut(s) 135
Mly113I GGCGCC 1 cut(s) 37
MlyI GAGTC 2 cut(s) 39, 168
MnlI CCTC 2 cut(s) 90, 292
Mph1103I ATGCAT 1 cut(s) 206
MroI TCCGGA 1 cut(s) 310
MslI CAYNNNNRTG 1 cut(s) 209
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 1 cut(s) 332
MvaI CCWGG 1 cut(s) 30
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 1 cut(s) 34
NarI GGCGCC 1 cut(s) 37
NdeI CATATG 2 cut(s) 89, 221
NlaIII CATG 3 cut(s) 194, 204, 214
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 1 cut(s) 262
NsiI ATGCAT 1 cut(s) 206
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 2 cut(s) 61, 118
PleI GAGTC 2 cut(s) 39, 168
PluTI GGCGCC 1 cut(s) 40
PpsI GAGTC 2 cut(s) 39, 168
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 1 cut(s) 227
RseI CAYNNNNRTG 1 cut(s) 209
SatI GCNGC 2 cut(s) 60, 117
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 2 cut(s) 39, 168
ScrFI CCNGG 1 cut(s) 30
SetI ASST 2 cut(s) 129, 274
SfoI GGCGCC 1 cut(s) 38
SinI GGWCC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 209
Sse9I AATT 1 cut(s) 135
SsiI CCGC 3 cut(s) 59, 117, 215
SspDI GGCGCC 1 cut(s) 36
StyD4I CCNGG 1 cut(s) 28
StyI CCWWGG 1 cut(s) 230
TaaI ACNGT 2 cut(s) 21, 321
TaiI ACGT 1 cut(s) 274
TaqI TCGA 1 cut(s) 257
TasI AATT 1 cut(s) 135
TauI GCSGC 2 cut(s) 62, 119
TfiI GAWTC 1 cut(s) 251
TscAI CASTG 3 cut(s) 24, 81, 324
TseFI GTSAC 1 cut(s) 262
Tsp45I GTSAC 1 cut(s) 262
TspDTI ATGAA 1 cut(s) 189
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 3 cut(s) 24, 81, 324
VpaK11BI GGWCC 1 cut(s) 227
ZraI GACGTC 1 cut(s) 272
Zsp2I ATGCAT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.