Rw2G003200

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0013780
Physical Location & Seq
Reverse (-)
3025362 .. 3026240
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G003200.1

Sequence Viewer

Length: 879 bp
ATGCCATACTTCAAGTCACTGTCTCTGCCTGGAAGGCGCCGAAGACTCAACCCGAGAGCGGCCAATGACTTCACACTGCCTCTCGTCATATGGCAATATATTCTTCAGTTTGTTGCCGCCGATGACCTATACACAATTCTAAAGTGTAGGAGTGTTTGTAAGACTTTTATGAGACTCATTGATGACTTCCATGTCTATTCATGCATAGACATGGCGGGCATATGTGGTCCTTGGGATATTGACATTTGTGAATCATCGAAAGTGACGCGACGTCGCCTGTTTGCTAAGCAATGCTTGGAGGCGAATAATCCGGAAGCACTGTTCCGTCAGGCATTCCGAACCATGAAGAAGTCCGGCAACATTCCCATGGCAACCCAATACTTACAAATGGCATGCAGGGGGGGCACAATCTTTAAAGGTTGGTATAAGTGGATTGACTACCACGACATTGCGTATTATGTGTTGTGCATGTTGCAGATCATCAGGGGAAACCAGGAGGTCCGAAATAAGACACTCAAAGACATGGTTGGTCGGTTGGATTGGAGGAAAAGTGGCTGGGAAGTACAATATTGTCGACATGAGGCTCACAGATGGTTGGATATTGGGCAGCCGACGCACTCCGATGTCTTGAAACACATCCCTACCGACTACTCATTCCTTCCGGAACGATGTCAGGGTTGCGGTGAACATTCGTTCCACCGTCCGTTCTTACATAACATGTGGTTAGTTAACTCTGATAGGAATATGGGTGAAACTTGTGATGTTTGCAAGTGCTACATCGAGGCCTCAAATTTCTACTACAAGCTACGGAATTCGGCATCCTCATTCCAACACCTGCTCGATTGGTATGCATTCATTGACGACATTCCCCGCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

292

Amino Acids

34.54

Weight (kDa)

8.87

Isoelectric Point (pI)

47.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_27 PF23310 90 - 197 1.8e-10 Plant tetratrico peptide repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 843
AasI GACNNNNNNGTC 1 cut(s) 191
AatII GACGTC 1 cut(s) 274
Acc36I ACCTGC 1 cut(s) 843
AccB1I GGYRCC 1 cut(s) 36
AccBSI CCGCTC 1 cut(s) 59
AccI GTMKAC 1 cut(s) 574
AccII CGCG 1 cut(s) 268
AccIII TCCGGA 2 cut(s) 310, 661
AciI CCGC 5 cut(s) 59, 117, 215, 681, 871
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 790, 811
AcuI CTGAAG 1 cut(s) 89
AcyI GRCGYC 2 cut(s) 37, 271
AfaI GTAC 1 cut(s) 564
AfiI CCNNNNNNNGG 1 cut(s) 58
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 2 cut(s) 13, 631
AhdI GACNNNNNGTC 1 cut(s) 270
AjnI CCWGG 2 cut(s) 28, 492
AloI GAACNNNNNNTCC 4 cut(s) 305, 337, 678, 710
AluBI AGCT 1 cut(s) 805
AluI AGCT 1 cut(s) 805
Alw26I GTCTC 2 cut(s) 27, 166
Ama87I CYCGRG 1 cut(s) 52
Aor13HI TCCGGA 2 cut(s) 310, 661
AoxI GGCC 2 cut(s) 60, 783
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 2 cut(s) 790, 811
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 2 cut(s) 227, 499
AsuHPI GGTGA 2 cut(s) 695, 761
AvaI CYCGRG 1 cut(s) 52
AvaII GGWCC 2 cut(s) 227, 499
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 619
BccI CCATC 1 cut(s) 585
BcgI CGANNNNNNTGC 2 cut(s) 830, 864
BciT130I CCWGG 2 cut(s) 30, 494
BcoDI GTCTC 2 cut(s) 27, 166
BfoI RGCGCY 1 cut(s) 40
BfuAI ACCTGC 1 cut(s) 843
BglI GCCNNNNNGGC 1 cut(s) 34
BisI GCNGC 3 cut(s) 60, 117, 608
BlpI GCTNAGC 1 cut(s) 285
BlsI GCNGC 3 cut(s) 61, 118, 609
Bme1390I CCNGG 2 cut(s) 30, 494
Bme18I GGWCC 2 cut(s) 227, 499
BmeRI GACNNNNNGTC 1 cut(s) 270
BmeT110I CYCGRG 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 227, 499
BmiI GGNNCC 1 cut(s) 38
BmrFI CCNGG 2 cut(s) 30, 494
BmsI GCATC 1 cut(s) 827
BpiI GAAGAC 1 cut(s) 49
Bpu1102I GCTNAGC 1 cut(s) 285
BsaHI GRCGYC 2 cut(s) 37, 271
BsaJI CCNNGG 2 cut(s) 230, 366
BsaWI WCCGGW 2 cut(s) 310, 661
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 535, 565
Bsc4I CCNNNNNNNGG 1 cut(s) 58
Bse3DI GCAATG 2 cut(s) 296, 447
BseAI TCCGGA 2 cut(s) 310, 661
BseBI CCWGG 2 cut(s) 30, 494
BseDI CCNNGG 2 cut(s) 230, 366
BseGI GGATG 2 cut(s) 636, 818
BseLI CCNNNNNNNGG 1 cut(s) 58
BseMI GCAATG 2 cut(s) 296, 447
BseSI GKGCMC 1 cut(s) 407
BseXI GCAGC 1 cut(s) 619
BseYI CCCAGC 1 cut(s) 555
Bsh1236I CGCG 1 cut(s) 268
BshFI GGCC 2 cut(s) 62, 785
BshNI GGYRCC 1 cut(s) 36
BsiHKCI CYCGRG 1 cut(s) 52
BsiSI CCGG 3 cut(s) 311, 354, 662
BslI CCNNNNNNNGG 1 cut(s) 58
BsmAI GTCTC 2 cut(s) 27, 166
BsmI GAATGC 2 cut(s) 332, 851
BsnI GGCC 2 cut(s) 62, 785
BsoBI CYCGRG 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 407
Bsp13I TCCGGA 2 cut(s) 310, 661
Bsp143I GATC 1 cut(s) 477
Bsp1720I GCTNAGC 1 cut(s) 285
Bsp19I CCATGG 1 cut(s) 366
BspACI CCGC 5 cut(s) 59, 117, 215, 681, 871
BspANI GGCC 2 cut(s) 62, 785
BspEI TCCGGA 2 cut(s) 310, 661
BspFNI CGCG 1 cut(s) 268
BspLI GGNNCC 1 cut(s) 38
BspMI ACCTGC 1 cut(s) 843
BspT107I GGYRCC 1 cut(s) 36
BsrBI CCGCTC 1 cut(s) 59
BsrDI GCAATG 2 cut(s) 296, 447
BssECI CCNNGG 2 cut(s) 230, 366
BssMI GATC 1 cut(s) 477
BssNI GRCGYC 2 cut(s) 37, 271
BssT1I CCWWGG 2 cut(s) 230, 366
Bst2UI CCWGG 2 cut(s) 30, 494
Bst4CI ACNGT 3 cut(s) 21, 321, 701
BstACI GRCGYC 2 cut(s) 37, 271
BstC8I GCNNGC 2 cut(s) 217, 394
BstDEI CTNAG 1 cut(s) 285
BstDSI CCRYGG 1 cut(s) 366
BstF5I GGATG 2 cut(s) 636, 818
BstFNI CGCG 1 cut(s) 268
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 1 cut(s) 480
BstMAI GTCTC 2 cut(s) 27, 166
BstMBI GATC 1 cut(s) 477
BstMWI GCNNNNNNNGC 3 cut(s) 34, 402, 613
BstNI CCWGG 2 cut(s) 30, 494
BstNSI RCATGY 3 cut(s) 396, 472, 721
BstSCI CCNGG 2 cut(s) 28, 492
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 1 cut(s) 268
BstV1I GCAGC 1 cut(s) 619
BstV2I GAAGAC 1 cut(s) 49
BsuRI GGCC 2 cut(s) 62, 785
BtgI CCRYGG 1 cut(s) 366
BtsCI GGATG 2 cut(s) 636, 818
BtsI GCAGTG 1 cut(s) 74
BtsIMutI CAGTG 3 cut(s) 17, 74, 317
BveI ACCTGC 1 cut(s) 843
Cac8I GCNNGC 2 cut(s) 217, 394
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 2 cut(s) 227, 499
CseI GACGC 2 cut(s) 274, 622
Csp6I GTAC 1 cut(s) 563
CviJI RGCY 6 cut(s) 62, 555, 584, 610, 785, 805
CviKI_1 RGCY 6 cut(s) 62, 555, 584, 610, 785, 805
CviQI GTAC 1 cut(s) 563
DdeI CTNAG 1 cut(s) 285
DinI GGCGCC 1 cut(s) 38
DpnI GATC 1 cut(s) 479
DpnII GATC 1 cut(s) 477
DraI TTTAAA 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 191
DriI GACNNNNNGTC 1 cut(s) 270
DseDI GACNNNNNNGTC 1 cut(s) 191
EaeI YGGCCR 1 cut(s) 60
Eam1105I GACNNNNNGTC 1 cut(s) 270
Eco130I CCWWGG 2 cut(s) 230, 366
Eco147I AGGCCT 1 cut(s) 785
Eco47I GGWCC 2 cut(s) 227, 499
Eco57I CTGAAG 1 cut(s) 89
Eco88I CYCGRG 1 cut(s) 52
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 2 cut(s) 28, 492
EcoT14I CCWWGG 2 cut(s) 230, 366
EcoT22I ATGCAT 2 cut(s) 206, 853
EgeI GGCGCC 1 cut(s) 38
EheI GGCGCC 1 cut(s) 38
ErhI CCWWGG 2 cut(s) 230, 366
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 2 cut(s) 89, 221
FblI GTMKAC 1 cut(s) 574
Fnu4HI GCNGC 3 cut(s) 60, 117, 608
FokI GGATG 2 cut(s) 623, 805
Fsp4HI GCNGC 3 cut(s) 60, 117, 608
GlaI GCGC 1 cut(s) 38
GluI GCNGC 3 cut(s) 60, 117, 608
GsaI CCCAGC 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 62, 785
HapII CCGG 3 cut(s) 311, 354, 662
HgaI GACGC 2 cut(s) 274, 622
HhaI GCGC 1 cut(s) 39
Hin1I GRCGYC 2 cut(s) 37, 271
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 2 cut(s) 575, 730
HindII GTYRAC 2 cut(s) 575, 730
HinfI GANTC 3 cut(s) 45, 174, 251
HpaI GTTAAC 1 cut(s) 730
HpaII CCGG 3 cut(s) 311, 354, 662
HphI GGTGA 2 cut(s) 695, 761
Hpy166II GTNNAC 3 cut(s) 575, 686, 730
Hpy188I TCNGA 5 cut(s) 338, 503, 622, 736, 878
Hpy188III TCNNGA 3 cut(s) 311, 628, 662
Hpy8I GTNNAC 3 cut(s) 575, 686, 730
Hpy99I CGWCG 3 cut(s) 273, 276, 616
HpyAV CCTTC 2 cut(s) 27, 668
HpyCH4III ACNGT 3 cut(s) 21, 321, 701
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 6 cut(s) 204, 396, 468, 475, 768, 851
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 402, 613
HpyF3I CTNAG 1 cut(s) 285
HpySE526I ACGT 1 cut(s) 271
Hsp92I GRCGYC 2 cut(s) 37, 271
HspAI GCGC 1 cut(s) 37
KasI GGCGCC 1 cut(s) 36
Kpn2I TCCGGA 2 cut(s) 310, 661
KspAI GTTAAC 1 cut(s) 730
Kzo9I GATC 1 cut(s) 477
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 1 cut(s) 827
MaeII ACGT 1 cut(s) 271
MaeIII GTNAC 2 cut(s) 15, 262
MalI GATC 1 cut(s) 479
MbiI CCGCTC 1 cut(s) 59
MboI GATC 1 cut(s) 477
MboII GAAGA 3 cut(s) 54, 95, 358
MhlI GDGCHC 1 cut(s) 407
MluCI AATT 3 cut(s) 135, 790, 811
Mly113I GGCGCC 1 cut(s) 37
MlyI GAGTC 2 cut(s) 39, 168
MmeI TCCRAC 3 cut(s) 516, 576, 853
MnlI CCTC 8 cut(s) 90, 292, 490, 537, 574, 775, 796, 832
Mph1103I ATGCAT 2 cut(s) 206, 853
MroI TCCGGA 2 cut(s) 310, 661
MseI TTAA 2 cut(s) 414, 729
MslI CAYNNNNRTG 3 cut(s) 209, 365, 621
MspI CCGG 3 cut(s) 311, 354, 662
MspR9I CCNGG 2 cut(s) 30, 494
Mva1269I GAATGC 2 cut(s) 332, 851
MvaI CCWGG 2 cut(s) 30, 494
MvnI CGCG 1 cut(s) 268
MwoI GCNNNNNNNGC 3 cut(s) 34, 402, 613
NarI GGCGCC 1 cut(s) 37
NcoI CCATGG 1 cut(s) 366
NdeI CATATG 2 cut(s) 89, 221
NdeII GATC 1 cut(s) 477
NlaIV GGNNCC 1 cut(s) 38
NmuCI GTSAC 2 cut(s) 15, 262
NsiI ATGCAT 2 cut(s) 206, 853
NspI RCATGY 3 cut(s) 396, 472, 721
PaeI GCATGC 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 843
PceI AGGCCT 1 cut(s) 785
PciI ACATGT 1 cut(s) 717
PctI GAATGC 2 cut(s) 332, 851
PfeI GAWTC 1 cut(s) 251
PkrI GCNGC 3 cut(s) 61, 118, 609
PleI GAGTC 2 cut(s) 39, 168
PluTI GGCGCC 1 cut(s) 40
PpsI GAGTC 2 cut(s) 39, 168
PscI ACATGT 1 cut(s) 717
Psp6I CCWGG 2 cut(s) 28, 492
PspFI CCCAGC 1 cut(s) 555
PspGI CCWGG 2 cut(s) 28, 492
PspN4I GGNNCC 1 cut(s) 38
PspPI GGNCC 2 cut(s) 227, 499
RsaI GTAC 1 cut(s) 564
RsaNI GTAC 1 cut(s) 563
RseI CAYNNNNRTG 3 cut(s) 209, 365, 621
SalI GTCGAC 1 cut(s) 573
SaqAI TTAA 2 cut(s) 414, 729
SatI GCNGC 3 cut(s) 60, 117, 608
Sau3AI GATC 1 cut(s) 477
Sau96I GGNCC 2 cut(s) 227, 499
SchI GAGTC 2 cut(s) 39, 168
ScrFI CCNGG 2 cut(s) 30, 494
SduI GDGCHC 1 cut(s) 407
SetI ASST 6 cut(s) 129, 274, 421, 501, 807, 837
SfaNI GCATC 1 cut(s) 827
SfoI GGCGCC 1 cut(s) 38
SinI GGWCC 2 cut(s) 227, 499
SmiMI CAYNNNNRTG 3 cut(s) 209, 365, 621
SphI GCATGC 1 cut(s) 396
Sse9I AATT 3 cut(s) 135, 790, 811
SseBI AGGCCT 1 cut(s) 785
SsiI CCGC 5 cut(s) 59, 117, 215, 681, 871
SspDI GGCGCC 1 cut(s) 36
SspI AATATT 1 cut(s) 569
StuI AGGCCT 1 cut(s) 785
StyD4I CCNGG 2 cut(s) 28, 492
StyI CCWWGG 2 cut(s) 230, 366
TaaI ACNGT 3 cut(s) 21, 321, 701
TaiI ACGT 1 cut(s) 274
TaqI TCGA 4 cut(s) 257, 574, 780, 840
TasI AATT 3 cut(s) 135, 790, 811
TatI WGTACW 1 cut(s) 562
TauI GCSGC 2 cut(s) 62, 119
TfiI GAWTC 1 cut(s) 251
Tru1I TTAA 2 cut(s) 414, 729
Tru9I TTAA 2 cut(s) 414, 729
TscAI CASTG 3 cut(s) 24, 81, 324
TseFI GTSAC 2 cut(s) 15, 262
TseI GCWGC 1 cut(s) 607
Tsp45I GTSAC 2 cut(s) 15, 262
TspDTI ATGAA 3 cut(s) 189, 359, 844
TspGWI ACGGA 3 cut(s) 314, 693, 823
TspRI CASTG 3 cut(s) 24, 81, 324
VpaK11BI GGWCC 2 cut(s) 227, 499
XapI RAATTY 2 cut(s) 790, 811
XceI RCATGY 3 cut(s) 396, 472, 721
XmiI GTMKAC 1 cut(s) 574
ZraI GACGTC 1 cut(s) 272
Zsp2I ATGCAT 2 cut(s) 206, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.