Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005928.1

Orthogroup ID

OG0006478

Physical Location & Seq

Genomic Coordinates Forward (+)

23107 .. 38684

Gene Structure 15578 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4149 bp

atggaatcatttttatgctcatatgttcaggtgttatggattctgcctctggcatttgctcagagttctataaagtccagtgttaccaggcattctgaagaatattgtgctatgcgtgacatttgtggtaaacgtactgatggaaaagtattgaactgtccgtacggttccccatctgtaaagccaagtgacttactttcatctaagatccaaagtttgtgtccaacaattactggaaatgtctgttgtacagcagcccaatttgacacattacggacatcggttcagcaagcaattccctttcttgtaggatgtccagcatgcttaagaaactttttgaacctcttctgtgagcttacctgctctccaaatcagagtttatttatcaatgtgacttctgtaaacaaggtaaataacaatatgaccgttggagggattgattattacataagtgatacttttggtgaggggttgtatgactcctgcaaggatgtcaagtttggtaccatgaattctcgagctatggaattcattggtgctggtgctcaaaattttaaagagtggtttacttttatcggtagacaagcagctcctaatctgccaggttcaccatatgccattagattccggtcaaatgctactgactcatccgagatgatacctatgaatgtatctacttattcttgtggtgataattcactgggctgttcctgtggtgattgcccgtcatccacagtttgttctaacacagctgttccagtttcccccaaaaaagggtcatgtacagtgaaaattggatctattaaggcaaaatgtattgacctttctgtcgccgttctatatatcgtactggtttctgtgttttttgggtggggtttgtttaatcggactaggaagcaaaccccggcttctaggaaaaaaccgttgtggaataatgtaatggatgatggcgaagttcttgaagatcctccccacatgagaagcagtgtccaactttcaattgtgcaaggatacatgtcaaaattttataggggatatgggaagtgggctgctagaaatccaatcacattgttgggttcatcgtctgctatagttcttctactttgtcttggtcttatccggttcaaggtggaaacaaggcctgagaagctatgggtagggccagggagtaaagcagccgaggagaagcaattttttgatagtcacctagcccctttttacagaattgagcagcttatattagcaacaataccagatgccaagcatggtaagtcacctagtattgtgacggaggacaacatcaagttactttttgagatacaaaaaaaggttgacggaattaaagcaaattattctgggtcaatgatatctcttgctgatatctgcatgaagccactagaccaagattgcgccagtcaaagtgtcatacagtatttcaaattggatcctgaaaattttgacaattatggtgggattgaacacctaacgtattgttttgagcactattcctctgctgacaaatgtatgagtgcatataaagctcctcttgatcccagcactgctttaggtggtttctctgggaaaaactattccgaggcatcagcatttataataacttatcctgtaaacaatgctattaataaagaggggaatgaaactgaaagagcagtggcatgggagaaggcctttattcagttggcaaaggctgagctgttgcaaatggtgcaatctaaaaacttaacactatctttctcatcggagagttccgttgaagaagaacttaagagagaaagcactgcagatgctattacaatattgataagctatcttgtgatgttcgcttatatttctcttactttgggtgactctcctcgtttgtcttccttttacatttcatcgaaggtattgctaggtctctctggagttgttcttgtcatgctctccgttcttggatcagtgggattgttcagtgccattggagttaaatctaccctaattattatggaagtcatcccatttcttgttctagctgttggggtagataatatgtgtatactggttcatgctgtcaaacggcaaccattgacgatgcctttagaagggcgaataagcaatgcacttgtggaagttggaccatccataactcttgctagtctttcagaagttttagcatttgcagttggcagctttattcctatgccagcttgtcgtgtgttctccatgtttgccgcactagctgttcttttggacttcctccttcaagttactgcttttgtagctctgattgttcttgattttcgccgaactgaggataatagggttgattgtttcccttgtatgaaagtttcatcatataccaactctgataaaggcattgaagtaagaaaaccaggtttgctggcacgctatatgaagggaatccatgctcccattctaagtgtttgggttgttaagatagttgtcattttcatttttgttgcgttttcactggctagcattgcgttgtgcaccagaattcagcctggtttggaacaaaaaatcgttcttccccaagattcctatcttcagggttatttcaataatatatcagcgtatctccgaattggacccccactatattttgttgtgaagaactacaactatagttcagaatcaagacatacaaaccaattgtgctccattagccaatgtgattcagattctcttttgaatgagattgcaagagcatccttaattccagaatcaagttacattgctaagcctgctgcttcatggcttgatgattttcttgtgtggatttccccagaagcttttgggtgctgtcggaagtttacaaatgcaacttattgtcctcctgatgatcagcctccttgctgttcttcctctgatggtacctgtagcctgggtggagtgtgcaaagattgtaccacgtgttttcgtcactcggatctgcataatggtcgtccgtcgaccacacaatttgaagaaaaacttccatggttcctcgatgctctgccctcttcagattgtgctaaaggtggccatggagcttacactagcagtgtggaattgaaaggctatgaaagtggcattattcaagcgtcatcatttcgtacatatcatacacctctcaacaaacagattgactatgtaaactcaatgagggctgctcgagagctctgttctagagtttctgattctttgaatatcgagatattcccatattcagtgttttacatgttctttgagcagtacctcgatatttggaaaacagcacttatgagcctttctatagctattggtgctgtatttatcgtttgcctagtcatcacatgcagcttgtggagttcagcaattatccttttggtgttggcaatgattgtcgtagatctcatgggagtgatggcaattcttcacattcaattaaatgcagtctctgttgttaaccttgttatggcagtgggcatttctgttgagttttgtgtgcatatgacacatgctttctcggttagcacaggagatagagatcaacgaataaaggaggctctgggtacaatgggtgcgtctgtcttcagtggaatcacacttacgaagttagttggggttattgtactttgtttctcaagaacagaagtctttgtggtgtattactttcaaatgtatctagcactagttcttctaggtttcttacatgggcttgtatttttgccggtggtgttgagcatttttgaatctgataggtttggcaagggagcggtctgtagagatcaccatggagtgtgtttggataagcttactacgccggggattggtttccctagtccactgacatgtgagaccatggcgcttgtgaatggactccatttctgtttgcaggctggattctcacactcggaagtggaaggggatgcacaaatggttttcaaggcattggcaactcttgatgaggatttgagtccagatggtgagttggtgaagaaagcaaagttttcactcggtctttttcaatcagttagttggggctatgtccctggaaacagtaacaaagcgacacattgtgtcgcaaattcagcactttctctacctcatcctacattttggtcgaggttgacgctggagtggctcagtcaaattatagtagatgagtaa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0005488 GO:0005496 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005886 GO:0005887 GO:0006082 GO:0006464 GO:0006486 GO:0006629 GO:0006807 GO:0006810 GO:0006869 GO:0007034 GO:0007041 GO:0008150 GO:0008152 GO:0008202 GO:0008206 GO:0008289 GO:0009058 GO:0009059 GO:0009100 GO:0009101 GO:0009987 GO:0010876 GO:0012505 GO:0015485 GO:0015850 GO:0015918 GO:0016020 GO:0016021 GO:0019538 GO:0019752 GO:0030301 GO:0031224 GO:0031226 GO:0031967 GO:0031975 GO:0032787 GO:0032934 GO:0033036 GO:0034645 GO:0036094 GO:0036211 GO:0042592 GO:0042632 GO:0043170 GO:0043178 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043413 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0048471 GO:0048878 GO:0051179 GO:0051234 GO:0055088 GO:0055092 GO:0065007 GO:0065008 GO:0070085 GO:0071702 GO:0071704 GO:0071944 GO:0097159 GO:1901135 GO:1901137 GO:1901360 GO:1901564 GO:1901566 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

ko04142 ko04979 map04142 map04979

Pfam Domains

Protein Families

NPC1_N Patched

Protein Analysis

1382

Amino Acids

151.53

Weight (kDa)

6.32

Isoelectric Point (pI)

40.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NPC1_N	PF16414	34 - 263	1.8e-66	Niemann-Pick C1 N terminus
NPC1_MLD	PF22314	396 - 587	2.4e-38	NPC1, middle luminal domain
Patched	PF02460	610 - 763	1.5e-31	Patched SSD
Sterol-sensing	PF12349	631 - 781	5e-49	Sterol-sensing domain of SREBP cleavage-activation
RVT_3	PF13456	1249 - 1358	2e-15	Reverse transcriptase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006478)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G42470
fragaria_vesca	FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290 FvH4_5g25290
malus_domestica	MD06G1009400.v1.1
prunus_persica	Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1 Prupe.5G011100_v2.0.a1
pyrus_communis	pycom06g00780 pycom16g25890
rosa_chinensis	RchiOBHm_Chr7g0215501
rosa_laevigata	RLG00000002657
rosa_multiflora	Rmu_sc0005928.1_g000005
rosa_roxburghii	Rroxscaffold_3G00244190
rosa_rugosa	Rorug07G0154100 Rorug07G0154200
rosa_samantha	Rh7AG290300 Rh7BG282300 Rh7CG310300 Rh7DG294100
rosa_wichuraiana	Rw7G024790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1610
AasI	GACNNNNNNGTC	1 cut(s)	827
Acc36I	ACCTGC	1 cut(s)	368
Acc65I	GGTACC	2 cut(s)	503, 2891
AccB1I	GGYRCC	2 cut(s)	503, 2891
AccBSI	CCGCTC	1 cut(s)	3755
AccI	GTMKAC	3 cut(s)	580, 2053, 2969
AciI	CCGC	2 cut(s)	2229, 3755
AclWI	GGATC	8 cut(s)	202, 805, 959, 1439, 1452, 1544, 1960, 2955
AcoI	YGGCCR	1 cut(s)	3040
AcsI	RAATTY	7 cut(s)	511, 527, 550, 1022, 1453, 2514, 4066
AcuI	CTGAAG	4 cut(s)	117, 2549, 3006, 3556
AcvI	CACGTG	1 cut(s)	2931
AdeI	CACNNNGTG	1 cut(s)	4058
AfiI	CCNNNNNNNGG	8 cut(s)	432, 773, 774, 1126, 2099, 2308, 3454, 3809
AflII	CTTAAG	2 cut(s)	325, 1781
AflIII	ACRYGT	4 cut(s)	1014, 2930, 3237, 3830
AhlI	ACTAGT	1 cut(s)	3670
AjnI	CCWGG	7 cut(s)	86, 601, 1162, 2389, 2521, 2901, 4030
Alw21I	GWGCWC	5 cut(s)	547, 1503, 2510, 2678, 3180
Alw26I	GTCTC	3 cut(s)	1919, 3439, 3830
Alw44I	GTGCAC	1 cut(s)	2506
AlwI	GGATC	8 cut(s)	202, 805, 959, 1439, 1452, 1544, 1960, 2955
AlwNI	CAGNNNCTG	1 cut(s)	3437
Ama87I	CYCGRG	2 cut(s)	516, 3171
AoxI	GGCC	4 cut(s)	1139, 1160, 1683, 3040
ApaLI	GTGCAC	1 cut(s)	2506
ApeKI	GCWGC	9 cut(s)	254, 587, 1049, 1175, 1231, 2184, 2765, 3167, 3336
ApoI	RAATTY	7 cut(s)	511, 527, 550, 1022, 1453, 2514, 4066
ArsI	GACNNNNNNTTYG	2 cut(s)	205, 237
AseI	ATTAAT	1 cut(s)	1638
Asp700I	GAANNNNTTC	2 cut(s)	344, 2991
Asp718I	GGTACC	2 cut(s)	503, 2891
AspLEI	GCGC	2 cut(s)	1412, 3847
AspS9I	GGNCC	3 cut(s)	1160, 2132, 2606
AsuC2I	CCSGG	2 cut(s)	905, 3804
AsuNHI	GCTAGC	1 cut(s)	2492
AvaI	CYCGRG	2 cut(s)	516, 3171
AvaII	GGWCC	2 cut(s)	2132, 2606
BaeGI	GKGCMC	1 cut(s)	2510
BalI	TGGCCA	1 cut(s)	3042
BamHI	GGATCC	1 cut(s)	1444
BanI	GGYRCC	2 cut(s)	503, 2891
BanII	GRGCYC	1 cut(s)	3180
BbrPI	CACGTG	1 cut(s)	2931
BbsI	GAAGAC	2 cut(s)	1872, 3562
Bbv12I	GWGCWC	5 cut(s)	547, 1503, 2510, 2678, 3180
BbvI	GCAGC	9 cut(s)	266, 599, 1036, 1187, 1243, 2196, 2752, 3154, 3348
BccI	CCATC	7 cut(s)	134, 181, 941, 2143, 2882, 3397, 3956
BceAI	ACGGC	2 cut(s)	818, 2090
BcgI	CGANNNNNNTGC	4 cut(s)	1888, 1922, 2942, 2976
BciT130I	CCWGG	7 cut(s)	88, 603, 1164, 2391, 2523, 2903, 4032
BciVI	GTATCC	1 cut(s)	1004
BclI	TGATCA	1 cut(s)	2860
BcnI	CCSGG	2 cut(s)	905, 3804
BcoDI	GTCTC	3 cut(s)	1919, 3439, 3830
BcuI	ACTAGT	1 cut(s)	3670
BfmI	CTRYAG	6 cut(s)	1089, 1797, 2641, 2896, 3291, 3760
BfoI	RGCGCY	1 cut(s)	3848
BfrI	CTTAAG	2 cut(s)	325, 1781
BfuAI	ACCTGC	1 cut(s)	368
BfuI	GTATCC	1 cut(s)	1004
BglII	AGATCT	1 cut(s)	3388
BlpI	GCTNAGC	2 cut(s)	1707, 2757
Bme1390I	CCNGG	9 cut(s)	88, 603, 905, 1164, 2391, 2523, 2903, 3804, 4032
Bme18I	GGWCC	2 cut(s)	2132, 2606
BmeT110I	CYCGRG	2 cut(s)	516, 3171
BmgT120I	GGNCC	3 cut(s)	1160, 2132, 2606
BmiI	GGNNCC	6 cut(s)	169, 505, 1446, 2608, 2893, 3002
BmrFI	CCNGG	9 cut(s)	88, 603, 905, 1164, 2391, 2523, 2903, 3804, 4032
BmrI	ACTGGG	1 cut(s)	710
BmsI	GCATC	7 cut(s)	1246, 1607, 1792, 2079, 2735, 2998, 3898
BmtI	GCTAGC	1 cut(s)	2496
BmuI	ACTGGG	1 cut(s)	710
BpiI	GAAGAC	2 cut(s)	1872, 3562
BplI	GAGNNNNNCTC	2 cut(s)	3154, 3186
BpmI	CTGGAG	2 cut(s)	1941, 4136
Bpu1102I	GCTNAGC	2 cut(s)	1707, 2757
BpuEI	CTTGAG	1 cut(s)	3607
BpuMI	CCSGG	2 cut(s)	905, 3804
BsaAI	YACGTR	1 cut(s)	2931
BsaBI	GATNNNNATC	2 cut(s)	2559, 3525
BsaI	GGTCTC	2 cut(s)	1919, 3830
BsaWI	WCCGGW	2 cut(s)	627, 1119
BsaXI	ACNNNNNCTCC	2 cut(s)	349, 379
Bsc4I	CCNNNNNNNGG	8 cut(s)	432, 773, 774, 1126, 2099, 2308, 3454, 3809
Bse118I	RCCGGY	1 cut(s)	3709
Bse1I	ACTGG	8 cut(s)	78, 238, 705, 758, 855, 1413, 2061, 2493
Bse3DI	GCAATG	4 cut(s)	2119, 2496, 2751, 3381
Bse8I	GATNNNNATC	2 cut(s)	2559, 3525
BseBI	CCWGG	7 cut(s)	88, 603, 1164, 2391, 2523, 2903, 4032
BseJI	GATNNNNATC	2 cut(s)	2559, 3525
BseLI	CCNNNNNNNGG	8 cut(s)	432, 773, 774, 1126, 2099, 2308, 3454, 3809
BseMI	GCAATG	4 cut(s)	2119, 2496, 2751, 3381
BseMII	CTCAG	5 cut(s)	74, 1134, 1698, 2298, 4138
BseNI	ACTGG	8 cut(s)	78, 238, 705, 758, 855, 1413, 2061, 2493
BseRI	GAGGAG	3 cut(s)	1196, 1533, 1860
BseSI	GKGCMC	1 cut(s)	2510
BseXI	GCAGC	9 cut(s)	266, 599, 1036, 1187, 1243, 2196, 2752, 3154, 3348
BseYI	CCCAGC	1 cut(s)	1553
BshFI	GGCC	4 cut(s)	1141, 1162, 1685, 3042
BshNI	GGYRCC	2 cut(s)	503, 2891
BsiHKAI	GWGCWC	5 cut(s)	547, 1503, 2510, 2678, 3180
BsiHKCI	CYCGRG	2 cut(s)	516, 3171
BsiSI	CCGG	5 cut(s)	628, 905, 1120, 3710, 3803
BsiWI	CGTACG	1 cut(s)	162
BslFI	GGGAC	1 cut(s)	4013
BslI	CCNNNNNNNGG	8 cut(s)	432, 773, 774, 1126, 2099, 2308, 3454, 3809
BsmAI	GTCTC	3 cut(s)	1919, 3439, 3830
BsmFI	GGGAC	1 cut(s)	4013
BsmI	GAATGC	1 cut(s)	91
BsnI	GGCC	4 cut(s)	1141, 1162, 1685, 3042
Bso31I	GGTCTC	2 cut(s)	1919, 3830
BsoBI	CYCGRG	2 cut(s)	516, 3171
Bsp1286I	GDGCHC	5 cut(s)	547, 1503, 2510, 2678, 3180
Bsp1407I	TGTACA	2 cut(s)	248, 782
Bsp1720I	GCTNAGC	2 cut(s)	1707, 2757
Bsp19I	CCATGG	4 cut(s)	2996, 3043, 3772, 3840
BspACI	CCGC	2 cut(s)	2229, 3755
BspANI	GGCC	4 cut(s)	1141, 1162, 1685, 3042
BspCNI	CTCAG	5 cut(s)	73, 1135, 1699, 2299, 4137
BspLI	GGNNCC	6 cut(s)	169, 505, 1446, 2608, 2893, 3002
BspMAI	CTGCAG	1 cut(s)	1801
BspMI	ACCTGC	1 cut(s)	368
BspOI	GCTAGC	1 cut(s)	2496
BspPI	GGATC	8 cut(s)	202, 805, 959, 1439, 1452, 1544, 1960, 2955
BspT107I	GGYRCC	2 cut(s)	503, 2891
BspTI	CTTAAG	2 cut(s)	325, 1781
BspTNI	GGTCTC	2 cut(s)	1919, 3830
BsrBI	CCGCTC	1 cut(s)	3755
BsrDI	GCAATG	4 cut(s)	2119, 2496, 2751, 3381
BsrFI	RCCGGY	1 cut(s)	3709
BsrGI	TGTACA	2 cut(s)	248, 782
BsrI	ACTGG	8 cut(s)	78, 238, 705, 758, 855, 1413, 2061, 2493
BssAI	RCCGGY	1 cut(s)	3709
BssNAI	GTATAC	1 cut(s)	2054
BssT1I	CCWWGG	4 cut(s)	2996, 3043, 3772, 3840
Bst1107I	GTATAC	1 cut(s)	2054
Bst2UI	CCWGG	7 cut(s)	88, 603, 1164, 2391, 2523, 2903, 4032
Bst4CI	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1431, 4040
Bst6I	CTCTTC	2 cut(s)	350, 3025
BstAFI	CTTAAG	2 cut(s)	325, 1781
BstAPI	GCANNNNNTGC	1 cut(s)	1723
BstAUI	TGTACA	2 cut(s)	248, 782
BstBAI	YACGTR	1 cut(s)	2931
BstC8I	GCNNGC	8 cut(s)	291, 322, 2202, 2400, 2404, 2494, 2763, 3876
BstDEI	CTNAG	8 cut(s)	60, 204, 1143, 1707, 2307, 2435, 2757, 4124
BstDSI	CCRYGG	4 cut(s)	2996, 3043, 3772, 3840
BstENI	CCTNNNNNAGG	1 cut(s)	2097
BstH2I	RGCGCY	1 cut(s)	3848
BstHHI	GCGC	2 cut(s)	1412, 3847
BstMAI	GTCTC	3 cut(s)	1919, 3439, 3830
BstNI	CCWGG	7 cut(s)	88, 603, 1164, 2391, 2523, 2903, 4032
BstNSI	RCATGY	6 cut(s)	324, 1018, 3241, 3336, 3500, 3834
BstSCI	CCNGG	9 cut(s)	86, 601, 903, 1162, 2389, 2521, 2901, 3802, 4030
BstSFI	CTRYAG	6 cut(s)	1089, 1797, 2641, 2896, 3291, 3760
BstSLI	GKGCMC	1 cut(s)	2510
BstV1I	GCAGC	9 cut(s)	266, 599, 1036, 1187, 1243, 2196, 2752, 3154, 3348
BstV2I	GAAGAC	2 cut(s)	1872, 3562
BstX2I	RGATCY	6 cut(s)	207, 797, 964, 1444, 2947, 3388
BstYI	RGATCY	6 cut(s)	207, 797, 964, 1444, 2947, 3388
BstZ17I	GTATAC	1 cut(s)	2054
BsuI	GTATCC	1 cut(s)	1004
BsuRI	GGCC	4 cut(s)	1141, 1162, 1685, 3042
BtgI	CCRYGG	4 cut(s)	2996, 3043, 3772, 3840
BtsI	GCAGTG	6 cut(s)	991, 1557, 1674, 1794, 3067, 3465
BveI	ACCTGC	1 cut(s)	368
Cac8I	GCNNGC	8 cut(s)	291, 322, 2202, 2400, 2404, 2494, 2763, 3876
CaiI	CAGNNNCTG	1 cut(s)	3437
CfoI	GCGC	2 cut(s)	1412, 3847
Cfr10I	RCCGGY	1 cut(s)	3709
Cfr13I	GGNCC	3 cut(s)	1160, 2132, 2606
CseI	GACGC	3 cut(s)	3090, 3552, 4120
CsiI	ACCWGGT	1 cut(s)	2389
CspCI	CAANNNNNGTGG	4 cut(s)	1450, 1485, 2962, 2997
DdeI	CTNAG	8 cut(s)	60, 204, 1143, 1707, 2307, 2435, 2757, 4124
DraI	TTTAAA	1 cut(s)	556
DraIII	CACNNNGTG	1 cut(s)	4058
DrdI	GACNNNNNNGTC	1 cut(s)	827
DseDI	GACNNNNNNGTC	1 cut(s)	827
EaeI	YGGCCR	1 cut(s)	3040
Eam1104I	CTCTTC	2 cut(s)	350, 3025
EarI	CTCTTC	2 cut(s)	350, 3025
Ecl136II	GAGCTC	1 cut(s)	3178
Eco130I	CCWWGG	4 cut(s)	2996, 3043, 3772, 3840
Eco147I	AGGCCT	2 cut(s)	1141, 1685
Eco24I	GRGCYC	1 cut(s)	3180
Eco31I	GGTCTC	2 cut(s)	1919, 3830
Eco32I	GATATC	2 cut(s)	1368, 1381
Eco47I	GGWCC	2 cut(s)	2132, 2606
Eco53kI	GAGCTC	1 cut(s)	3178
Eco57I	CTGAAG	4 cut(s)	117, 2549, 3006, 3556
Eco72I	CACGTG	1 cut(s)	2931
Eco88I	CYCGRG	2 cut(s)	516, 3171
EcoICRI	GAGCTC	1 cut(s)	3178
EcoNI	CCTNNNNNAGG	1 cut(s)	2097
EcoRI	GAATTC	3 cut(s)	511, 527, 2514
EcoRII	CCWGG	7 cut(s)	86, 601, 1162, 2389, 2521, 2901, 4030
EcoRV	GATATC	2 cut(s)	1368, 1381
EcoT14I	CCWWGG	4 cut(s)	2996, 3043, 3772, 3840
EcoT38I	GRGCYC	1 cut(s)	3180
ErhI	CCWWGG	4 cut(s)	2996, 3043, 3772, 3840
FalI	AAGNNNNNCTT	8 cut(s)	442, 474, 1530, 1562, 1764, 1796, 2976, 3008
FaqI	GGGAC	1 cut(s)	4013
FauNDI	CATATG	3 cut(s)	22, 613, 3489
FbaI	TGATCA	1 cut(s)	2860
FblI	GTMKAC	3 cut(s)	580, 2053, 2969
FriOI	GRGCYC	1 cut(s)	3180
GlaI	GCGC	2 cut(s)	1411, 3846
GsaI	CCCAGC	1 cut(s)	1557
GsuI	CTGGAG	2 cut(s)	1941, 4136
HaeII	RGCGCY	1 cut(s)	3848
HaeIII	GGCC	4 cut(s)	1141, 1162, 1685, 3042
HapII	CCGG	5 cut(s)	628, 905, 1120, 3710, 3803
HgaI	GACGC	3 cut(s)	3090, 3552, 4120
HhaI	GCGC	2 cut(s)	1412, 3847
Hin6I	GCGC	2 cut(s)	1410, 3845
HinP1I	GCGC	2 cut(s)	1410, 3845
HincII	GTYRAC	4 cut(s)	1333, 2970, 3445, 4110
HindII	GTYRAC	4 cut(s)	1333, 2970, 3445, 4110
HindIII	AAGCTT	2 cut(s)	2808, 3791
HpaI	GTTAAC	1 cut(s)	3445
HpaII	CCGG	5 cut(s)	628, 905, 1120, 3710, 3803
Hpy99I	CGWCG	1 cut(s)	2971
HpyAV	CCTTC	6 cut(s)	1675, 1894, 2093, 2267, 2407, 3896
HpyCH4III	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1431, 4040
HpyCH4IV	ACGT	3 cut(s)	133, 1487, 2930
HpyF3I	CTNAG	8 cut(s)	60, 204, 1143, 1707, 2307, 2435, 2757, 4124
HpySE526I	ACGT	3 cut(s)	133, 1487, 2930
HspAI	GCGC	2 cut(s)	1410, 3845
KpnI	GGTACC	2 cut(s)	507, 2895
Ksp22I	TGATCA	1 cut(s)	2860
KspAI	GTTAAC	1 cut(s)	3445
LmnI	GCTCC	6 cut(s)	595, 1546, 2431, 2681, 3047, 3752
Lsp1109I	GCAGC	9 cut(s)	266, 599, 1036, 1187, 1243, 2196, 2752, 3154, 3348
LweI	GCATC	7 cut(s)	1246, 1607, 1792, 2079, 2735, 2998, 3898
MabI	ACCWGGT	1 cut(s)	2389
MaeII	ACGT	3 cut(s)	133, 1487, 2930
MbiI	CCGCTC	1 cut(s)	3755
MfeI	CAATTG	2 cut(s)	999, 2669
MflI	RGATCY	6 cut(s)	207, 797, 964, 1444, 2947, 3388
MhlI	GDGCHC	5 cut(s)	547, 1503, 2510, 2678, 3180
MlsI	TGGCCA	1 cut(s)	3042
MluNI	TGGCCA	1 cut(s)	3042
MlyI	GAGTC	5 cut(s)	473, 638, 1859, 3852, 3964
MmeI	TCCRAC	5 cut(s)	248, 409, 1015, 2110, 2804
Mox20I	TGGCCA	1 cut(s)	3042
MroXI	GAANNNNTTC	2 cut(s)	344, 2991
MscI	TGGCCA	1 cut(s)	3042
MslI	CAYNNNNRTG	3 cut(s)	13, 3398, 3829
Msp20I	TGGCCA	1 cut(s)	3042
MspA1I	CMGCKG	1 cut(s)	752
MspCI	CTTAAG	2 cut(s)	325, 1781
MspI	CCGG	5 cut(s)	628, 905, 1120, 3710, 3803
MspR9I	CCNGG	9 cut(s)	88, 603, 905, 1164, 2391, 2523, 2903, 3804, 4032
MunI	CAATTG	2 cut(s)	999, 2669
Mva1269I	GAATGC	1 cut(s)	91
MvaI	CCWGG	7 cut(s)	88, 603, 1164, 2391, 2523, 2903, 4032
NciI	CCSGG	2 cut(s)	905, 3804
NcoI	CCATGG	4 cut(s)	2996, 3043, 3772, 3840
NdeI	CATATG	3 cut(s)	22, 613, 3489
NheI	GCTAGC	1 cut(s)	2492
NlaIV	GGNNCC	6 cut(s)	169, 505, 1446, 2608, 2893, 3002
NmeAIII	GCCGAG	1 cut(s)	1204
NmuCI	GTSAC	8 cut(s)	116, 188, 391, 1202, 1272, 1285, 1862, 2939
NspI	RCATGY	6 cut(s)	324, 1018, 3241, 3336, 3500, 3834
PaeI	GCATGC	1 cut(s)	324
PaeR7I	CTCGAG	2 cut(s)	516, 3171
PceI	AGGCCT	2 cut(s)	1141, 1685
PciI	ACATGT	3 cut(s)	1014, 3237, 3830
PctI	GAATGC	1 cut(s)	91
PdmI	GAANNNNTTC	2 cut(s)	344, 2991
Pfl23II	CGTACG	1 cut(s)	162
PleI	GAGTC	5 cut(s)	473, 638, 1859, 3852, 3963
PmaCI	CACGTG	1 cut(s)	2931
PmlI	CACGTG	1 cut(s)	2931
PpsI	GAGTC	5 cut(s)	473, 638, 1859, 3852, 3963
Ppu21I	YACGTR	1 cut(s)	2931
PscI	ACATGT	3 cut(s)	1014, 3237, 3830
PshBI	ATTAAT	1 cut(s)	1638
PsiI	TTATAA	1 cut(s)	1610
Psp124BI	GAGCTC	1 cut(s)	3180
Psp6I	CCWGG	7 cut(s)	86, 601, 1162, 2389, 2521, 2901, 4030
PspCI	CACGTG	1 cut(s)	2931
PspFI	CCCAGC	1 cut(s)	1553
PspGI	CCWGG	7 cut(s)	86, 601, 1162, 2389, 2521, 2901, 4030
PspLI	CGTACG	1 cut(s)	162
PspN4I	GGNNCC	6 cut(s)	169, 505, 1446, 2608, 2893, 3002
PspPI	GGNCC	3 cut(s)	1160, 2132, 2606
PsrI	GAACNNNNNNTAC	2 cut(s)	146, 178
PstI	CTGCAG	1 cut(s)	1801
PstNI	CAGNNNCTG	1 cut(s)	3437
PsuI	RGATCY	6 cut(s)	207, 797, 964, 1444, 2947, 3388
PvuII	CAGCTG	1 cut(s)	752
RseI	CAYNNNNRTG	3 cut(s)	13, 3398, 3829
SacI	GAGCTC	1 cut(s)	3180
SalI	GTCGAC	1 cut(s)	2968
Sau96I	GGNCC	3 cut(s)	1160, 2132, 2606
SchI	GAGTC	5 cut(s)	473, 638, 1859, 3852, 3964
ScrFI	CCNGG	9 cut(s)	88, 603, 905, 1164, 2391, 2523, 2903, 3804, 4032
SduI	GDGCHC	5 cut(s)	547, 1503, 2510, 2678, 3180
SexAI	ACCWGGT	1 cut(s)	2389
SfaNI	GCATC	7 cut(s)	1246, 1607, 1792, 2079, 2735, 2998, 3898
SfcI	CTRYAG	6 cut(s)	1089, 1797, 2641, 2896, 3291, 3760
Sfr274I	CTCGAG	2 cut(s)	516, 3171
SinI	GGWCC	2 cut(s)	2132, 2606
SlaI	CTCGAG	2 cut(s)	516, 3171
SmiMI	CAYNNNNRTG	3 cut(s)	13, 3398, 3829
SmlI	CTYRAG	5 cut(s)	325, 516, 1781, 3171, 3622
SmoI	CTYRAG	5 cut(s)	325, 516, 1781, 3171, 3622
SpeI	ACTAGT	1 cut(s)	3670
SphI	GCATGC	1 cut(s)	324
SseBI	AGGCCT	2 cut(s)	1141, 1685
SsiI	CCGC	2 cut(s)	2229, 3755
SspI	AATATT	2 cut(s)	104, 1815
SstI	GAGCTC	1 cut(s)	3180
StuI	AGGCCT	2 cut(s)	1141, 1685
StyD4I	CCNGG	9 cut(s)	86, 601, 903, 1162, 2389, 2521, 2901, 3802, 4030
StyI	CCWWGG	4 cut(s)	2996, 3043, 3772, 3840
TaaI	ACNGT	8 cut(s)	158, 167, 427, 736, 787, 924, 1431, 4040
TaiI	ACGT	3 cut(s)	136, 1490, 2933
TaqI	TCGA	8 cut(s)	517, 1898, 2969, 3006, 3172, 3210, 3258, 4103
TaqII	GACCGA	1 cut(s)	3986
TatI	WGTACW	3 cut(s)	248, 782, 3610
TauI	GCSGC	1 cut(s)	2231
TseFI	GTSAC	8 cut(s)	116, 188, 391, 1202, 1272, 1285, 1862, 2939
TseI	GCWGC	9 cut(s)	254, 587, 1049, 1175, 1231, 2184, 2765, 3167, 3336
Tsp45I	GTSAC	8 cut(s)	116, 188, 391, 1202, 1272, 1285, 1862, 2939
TspGWI	ACGGA	7 cut(s)	150, 289, 1304, 1350, 1756, 1933, 2955
Vha464I	CTTAAG	2 cut(s)	325, 1781
VneI	GTGCAC	1 cut(s)	2506
VpaK11BI	GGWCC	2 cut(s)	2132, 2606
VspI	ATTAAT	1 cut(s)	1638
XagI	CCTNNNNNAGG	1 cut(s)	2097
XapI	RAATTY	7 cut(s)	511, 527, 550, 1022, 1453, 2514, 4066
XbaI	TCTAGA	1 cut(s)	3185
XceI	RCATGY	6 cut(s)	324, 1018, 3241, 3336, 3500, 3834
XhoI	CTCGAG	2 cut(s)	516, 3171
XmiI	GTMKAC	3 cut(s)	580, 2053, 2969
XmnI	GAANNNNTTC	2 cut(s)	344, 2991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.